", UN -' 0 ADMINISTRATIVE OFFICERS James E. Martin, President Paul F. Parks, Vice President for Research Lowell T. Frobish, Director David H. Teem, Associate Director Dale L. Huffman, Interim Associate Director Charles W. Bruce, Assistant Director COVER STORY: Tienrserirhiotry isone of A labarna's little k noon 'big businesses Th roughoiut the douad of the I -80sthe in- dustry~ showed slow. but steady growth. Research at theAlahamaAgriiultuua/Experi- mnint Station has playeod a ke'isro/e in the developmnen t and growthi of the nuisrsery in- dus tiy in the State. Mucnhi of the Experi ment Station nu rscery research is cdidscted at the Ornaniental Horticui/ture Subustation in Mobhi/e, site of an annuual N is rserc Field Dai. The corer photo shows Aidiurni researc hers at the /989 Fieldi Daisexp/ainiug tests design- ed to determn e the ty~pe and amniot of darnage done to nursery crops by various insects. 0 0 Foreword Introduction Food Animals Plant Production and Protection Forestry Food Technology and Nutrition Environmental Concerns Rural Life Alternative Crops and Resource Use Director's Research Awards Financial Report 91 The information contaie hel mrein is a-ui l o a lprsn regardless of race, color, sex, or national origin. This report was produced by the Department of Research Information R.E. Stevenson, Editor and Head J.R. Roberson, Associate Editor C.L. Smith, Assistant Editor T.E. Rodriguez, Art Designer 3 LA 9 Lowell T. Frobish, Director AAES World agricultural and forestry problems are linked so closely that an upset in one locality may be felt on the other side of the globe. The hazards of drought, flood, cold, in- sects, diseases, and other elements of nature impact global production of food and fiber. Global concerns in turn impact the agricultural and forest economy of Alabama. In the past, farmers and farm families may have been culturally distinctive, but improvements in communication and transportation have broken down many of our cultural barriers. Distinctions be- tween the farm and non-farm segments of the community are becoming nonexistent. Food and fiber production are influenced by a multiplicity of factors that were not present a few years past. This has caused a decrease in "full-time" farmers and resulted in more part- time farmers. Irrespective, one factor that has remained constant is the need for profitability. Today, producers of food and fiber are confronted with rules and regulations from all levels of government and with a society that demands an inexpensive, yet safe, food supply. Some public action groups already advocate the use of agricultural chemicals, synthetic fertilizers, growth promotants, to name few, not be reduced, but cease. The push for a return to the use of draft horses, organic ferti- lizers, and natural pest control is gaining momentum. Most people have heard such terms as LISA (Low Input Sustainable Agriculture), AlternativeAgriculture, Regenetive Agriculture, and other programs mentioned as substitutes for in- tensive production practices that require the use of pesticides and other valuable farm chemicals. While few would argue that reduced use of chemicals would be beneficial to man and the environ- ment, there must be a balance. Without farm chemicals, we could not produce the plentiful, safe food supply that Americans demand - not with the current distribution of farm and non-farm labor. Finding practical uses of biological, or naturally occurring compounds to fight pests and combat drought, freezes, and myriad production restraints that farmers face every- day is an ongoing challenge for Experiment Station research. The Alabama Agricultural Ex- periment Station is concerned about the environment and the conserva- tion and preservation of the State's natural resources. Our scientists are environmentalists. Their research emphasizes finding alternatives to many of today's production prac- tices. A balance between natural and chemical control of production pests is of major concern. This report summarizes our research ef- fort that is of benefit to all Ala- bamians. Greater emphasis is given to defining alternative production systems. Your input and interest in our programs are appreciated. You, the consumer, are an important part of our team. Our research programs are not designed for either large or small producers, but are size neutral. They benefit us all! ; L I NTRDUCiO Much of the research conducted by the Alabama Agricultural Ex- periment Station is highly visible and provides immediate techno- logical benefit to producers. Other work, known in the scientific com- munity as basic research, is more behind the scenes. While this work may not be immediately beneficial to producers, it may provide the stepping stone for the next genera- tion of new technology and benefit future generations of Alabamians. Biotechnology and Genetics are "hot" areas of research at universities nationwide. Last year the Experi- ment Station made giant strides in developing biotechnology pro- grams. Experiment Station research with transgenic fish, which contain genes from other fish, brought jour- nalists from as far away as Germany and Finland to report on this new tech- nology. Develop- ment of this techno- logy opens the way for inserting genes to improve resistance to disease and reduce susceptibility to a multitude of stress- related production problems. In related work, poultry researchers recently found that a specific gene func- tions differently in different genetic backgrounds. Large differential effects of genes designated B1 and B2 on Marek's disease (MD) in Leghorns were absent in broilers. B1 genes were resistant to MD and B2 genes were susceptible to it. In Leghorn-broiler crosses, the Bi pro- geny had one-half of the mortality from the 7 disease and twice the reproductive efficiency of their B2 siblings, indi- cating the effects ofagene are highly dependent upon the other genes pre- sent. In the Auburn test, the regulation of the ex- pression of an individual gene was as important as its individual structure. A Genetics research by d plant scientists is under- p way to identify and replicate DNA clones from soybean mitochon- dria. Results of these studies will provide a basic understanding of DNA replication pro- cesses in plant mitochon- dria, and may I ielp research- ers provide a basis for de- veloping don- ing vectors capable of in- I roducing foreign traits nto plants. In a multi-disciplinary study, researchers have completed the first step in genetically improving a o mycorrhizal fungus that benefits root systems of trees. These mychor- Ihizal fungi help plants .bsorb water and * utrients and to resist damage from root diseases, pollution, and other stress factors. Researchers intend to construct strains of the fungus with an improved ability to relieve plant stress. Improvements in the efficiency of these fungi to help produce healthier seedlings could provide an economic nimal research is geared to pro- ucing a safer food supply for onsumers and greater profit for roducers w ": .'p a . 'ildlife researchers are helping evelop both the recreational and usiness aspects of hunting and shing in the State. boost to the $63 billion a year forest industry in the Southeast. Much of the genetics research con- ducted by the Experi- ment Station will benefit from the opening of the Auburn University Cell Science Center. It is comprised of laboratory units for research on nonoclonal an- ibodies, fermenta- tion, and plant tissue culture. Specifically, research underway on hormone-induced gene expression and protein secretion in animal cells may have broad application in animal husbandry and human health and nutrition. Another project underway at the Center deals with molecular biology of nitrogen fixa- tion, which has direct application to plant biology and agricultural crops. The new tissue culture laboratories at the Center support work to develop a cold-hardy eucalyptus tree for use by the Southern pulp and paper industry. While some of these high tech results will be of immediate benefit to producers, others are likely to be more fully utilized in the future. However, the bulk of Experiment Station research is geared to help to- day and tomorrow. With such a diverse research program, it is im- possible to report on each endeavor. The following pages are dedicated to the highlights of some of the research results by the Alabama Agricultural Experiment Station in 1989. ., ' nei catfish with growth hormon genes from coldwater species int a specially protected pond near the Auburn campus I ~' Biotechnlology and genetics research have dramatic promise for future generations of Alabamians. de b fi eel A IM L labama is fortunate to have important beef cat- tle, dairy, swine, poultry, and catfish pro- duction centers scattered throughout the State. However, this diversity of food animal production requires a diverse research program to provide the technological base for continued growth. Not only must Experiment Station researchers work with a diversity of animals, they must likewise work in a number of production areas. Much of the animal research in- volves reproduction, nutrition, and physiology that provides the technology for producers to advance to the next level of pro- duction. For example, researchers recently developed an assay method that, when combined with chemical separation technology already available, will allow them to more closely determine the biochemical nature of reproductive tissue of heifers. Data from the tests indicate that factors produced by the uterus and placenta may influence the length of time of postpartum anestrous in beef cattle. Researchers are currently study- ing the role of 3-ribosyluric acid in protecting beef cattle from nitrite poisoning. Indications are that oxidation occurs in two phases and the addition of 3-ribosyluric acid in- creases oxidation time from about 10 minutes to about 3 hours. By understanding the mechanisms by which 3-ribosyluric acid works, researchers are hoping to develop more potent anti-oxidants. Catfish production continues to grow in Alabama, but disease remains a production obstacle. Edwardsiella ictaluri (ESC) is a serious disease problem of catfish that producers overcome by adding antibiotics to fish feed. Auburn researchers are currently working on getting Sarafloxacin, a new quinoline compound, approved for use on farmed fish. It has several advantages over other antibiotics for fish. It can be incorporated into Beet cattle and sheep usually don t mix, but recent research indicates the "woollies" may become a good cash crop for Alabama livestock producers. floating pellets because of its heat stability, it presents no palatability problems, it is highly effective against ESC, and it has a low ten- dency to cause antibiotic resistance in bacteria. Poultry remains a dominant food animal industry in the State, with new production pushing Alabama into second place, behind Arkansas, in broiler production. The con- tinued growth and technological advance in the industry provide many research challenges. Similar challenges are being met by dairy and swine researchers in the Experi- ment Station. Licorice-like Spray Improves Dairy Production Cows placed in new manage- ment groups based on nutritional considerations usually go through a period of 1-2 days of increased aggression during which a new social order is established. Masking individual odors by spraying anise oil, an olfactory nerve stimulant that smells like licorice, on all cows in a group resulted in decreased fighting in cows placed in new management groups. Masking odors with anise oil also eliminated the drop in milk production often observed in cows placed in new groups. New Drug More Effective on Coccidiosis-Caused Scours Coccidiosis is one cause of scours in newborn baby pigs. It has been previously reported by Experiment Station researchers that the drug Amprolium is beneficial in con- trolling scours caused by this organism. However, a new drug, Toltrazuril, was more effective than Amprolin in tests last year. Treatment of coccidiosis-infected pigs with toltrazuril at the dosage of 5 or 10 mg per kg of body weight resulted in these pigs gain- ing weight comparable to or better than the noninfected controls. Toltrazuril-treated pigs excreted few or no oocysts in the feces, reduc- ing the possibility of reinfection. If approved by the Federal Drug Administration for use in swine, the drug should provide a better control for one cause of scours in baby pigs. Reduced Light Improves Rooster Reproductivity Light plays an important role in regulation of reproduction in birds, including chickens. Most commer- cial light schedules are designed to stimulate egg production at an appropriate age. In natural mating systems, roosters are considered a necessary evil required for fertility of the egg and are exposed to long photoschedules used for hens. Flock fertility generally declines after roosters reach 48 weeks of age, and this reduction has been associated with excessive body weight or fat, leg problems, de- creased shell strength, diminished mating activity, or poor semen quality. Experiment Station research has shown that long light schedules may reduce semen pro- duction, spermatozoa quality, and possibly contribute to reduced fertility. Age at sexual maturity (ejacula- tion of first spermatozoa) was earliest for males given 8 hours of light. Body weight of the 8 hour group was lower than the other treatments, suggesting increased physical activity in this group. Testes weights, percent males pro- ducing semen, semen weight per ejaculate, and percent live sperma- tozoa were greater from roosters maintained on 8 or fewer hours of light compared to the groups on 16 or 24 hours of light. Phosphorus Source Affects Poultry Processing Problems Leg problems continue to be a concern for poultry producers in Alabama and across the country. Chickens that are fed nutritionally balanced diets and raised under op- timum management conditions, and which appear normal, often end up with broken legs and wings in the processing plants. The end result is condemnation or down- grading of poultry meat, which costs poultrymen and ultimately consumers. Experiment Station research in- dicates that the source of phosphorus in the diet of chickens may be the cause of some leg prob- lems. In poultry diets, calcium and phosphorus can be supplied from either organic or inorganic sources. Bone meal is an organic source, and its phosphorus has been considered to be 100 percent available to chickens. However, there are reports of variations ranging from 5 percent to 30 percent in, phosphorus availa- bility from bone meal. Because of these varia- tions, errors may be made when bone meal is included in diets for meat type chickens. In the Auburn research, source and particle size of bone meal were evaluated a, factors influencing phosphorus availabili- ty. Data were com- pared with those obtained using dicalcium phosphate, an inorganic phosphorus supplement of excellent phosphorus availability that is com- monly used in poultry diets, as the standard. Results indicated that there was no difference in phosphorus availability among bone meal sources. However, phosphorus from the different bone meal sources was 6-12 percent less available than that from dicalcium phosphate. This means that chickens fed organic phosphorus supplements might be receiving marginal levels of phosphorus, if availability values of 100 percent are used. The degree of deficiency would be dependent upon the percent of total dietary phosphorus supplied by the or- ganic sources. This research indicates that phosphorus availability values of 90-95 percent, instead of 100 percent, may be used in diets containing organic phosphorus sources to reduce otherwise unex- plained leg problems in chickens. Ip studies continue to l the continued growth poultry industry in th Portable X-Ray Valuable in Poultry Disease Diagnosis Tibial dyschondroplasia (TD) is a skeletal abnormality in poultry that costs the industry millions of dollars annually. While the exact source of TD is unknown, Experi- ment Station researchers have developed a non-lethal technique for diagnosis. A major obstacle in the past has been diagnostics, because the diagnosis either required killing suspect birds or employing im- mobile X-ray equipment. The new t . . developed at Auburn S- University employs a hand-held gun (Lix- iscope), that can easily and accurately diag- nose the condition, thereby allowing more precise genetic, nutri- tion, and management studies as they relate to TD. This technique has been used to success- , fully diagnose the oc- ay ii role lf TD of the currence of TD in e State. several thousand 3- to r 7-week-old broiler-type chicks in experiments at Auburn. New Vitamin C Source for Fish Feeds While most animals don't require vitamin C in their diet, fish are extremely sensitive to a deficiency. Without vitamin C, fish show reduced growth rate, physical deformities (crooked backs, etc.), slow wound healing, and reduced resistance to infections, environ- mental contaminants, and other stresses. L-ascorbic acid is the vitamin C source used in commercial fish feeds. It is sensitive to oxidation, and heat and moisture associated with feed processing destroy 40 to 60 percent of the amount put into the feed. Also, its half-life (time required for 50 percent to be lost) in fish feeds during storage is less than 90 days. Because of the large losses of ascorbic acid during processing and storage, new sources of vitamin C are needed for use in aquaculture feeds. Phosphate and sulfate deriva- tives of ascorbic acid, which are relatively stable against oxidative deterioration, were examined in Experiment Station research as potential sources of vitamin C for fish feeds. Ascorbic acid phosphate (L-ascorbyl-phosphate Mg), and ascorbic acid sulfate (L-ascorbyl- 2- sulfate) were compared with L- ascorbic acid for vitamin C activity for channel catfish. Each source of vitamin C was fed at four levels in purified diets under con- trolled laboratory con.d Fish fed no ascorbic acid (control) grew poorly. Those fed ascorbic acid sulfate also grew poorly and , although growth im- proved as the dietary level increased, it never reached the growth rate ' of the fish fed L- ascorbic acid. Growth rate of fish fed ascorbic acid phosphate was P equal to that of the fish n ill fed L-ascorbic acid. eeds of catfi This was true even at the lowest level fed, which is near the lower limit of the channel catfish's vitamin C requirements. There were no deformities in the fish fed any of the dietary levels of L-ascorbic acid or ascorbic acid phosphate. However, there was over 50 percent deformed fish, mainly crooked backs, among fish fed the control diet. Only the highest dose of ascorbic acid sulfate (31 mg per kg) prevented deformities in the fish. The results show that ascorbic acid phosphate is equal to L- ascorbic acid in meeting the vitamin C requirements of channel catfish, but that ascorbic acid sulfate has less vitamin C activity. During extrusion processing of catfish feed, only 10 to 20 percent of ascorbic acid phosphate was lost, while 50 to 60 percent of L-ascorbic acid was lost. Because of increased stability during processing and high vitamin C activity, ascorbic acid phosphate will likely be a major source of vitamin C in aquaculture feeds in the future. This will allow more precise sup- plementation of fish feeds with vitamin C, providing a cost savings and increased assurance that the feed is sufficient in vitamin C. Infected Fescue Reduces Heifer Pregnancy Rate Previous Experiment Station research conducted at the Black Belt and Tennessee Valley substations showed that pregnancy rates in heifers grazing fungus-infected tall educed60percent com- pared to heifers grazing S noninfected fescue. T Fhe differences were even more dramatic when the heifers were rebred following calv- ing. Only33 percent of 'he heifers grazing Snfected fescue became pregnant again, com- pared to 93 percent of those grazing non- nei n, ' infected fescue. Current research at the E.V. Smith Re- search Center is being conducted with an overall objective to learn why heifers did not become preg- nant. A study in which changes in blood hormone patterns were measured showed that heifers grazing infected fescue had normal reproductive cycles. An embryo transfer study was conducted in which embryos flush- ed from cows not grazing fescue were transferred to heifers grazing either infected or noninfected fescue. Only 31 percent of the re- cipient heifers grazing infected fescue had successful pregnancies compared to 73 percent of those grazing noninfected fescue. These results indicate that toxins in the fescue are altering the uterine environment so a successful pregnancy cannot be maintained. Catfish Models Provide Marketing Support Two new computer models were developed by Auburn researchers to benefit the State's growing cat- fish industry. A bioeconomic model was developed to help producers predict fish growth. The model uses water tempera- ture, body weight of the fish, feeding rates, and diet comp- osition as parameters for fish growth. Output from the fish growth model was used to generate a production function for five de- cision variables: stocking date, stocking weight, dietary protein percent, feeding rate, and crop length. Economic tradeoffs are demonstrated by the model. An econometric model for cat- fish was developed that takes into account several unique features of this successful, rapidly growing industry. These include: an im- perfectly competitive processing sector, rapid industry growth, a marketing problem known as off-flavor, and industry funded marketing efforts. The model con- tains three structural equations: a farm-level supply equation, a processor price-markup equation, and a wholesale-level demand equation and a farm level equation. rintinc s~ ir.,r LAN PRDCTO AN PROTETIN _ ,. .. .- ' , . - -' .M . t ar acterial ilsel-o ,ir a major vield-robbing problem for many crops grown in Alabama. Not only does this group of diseases reduce productivity, but growers spend millions of dollars annually to protect their crop from them. Last year Experiment Station research made a major breakthrough in understanding bacterial diseases of plants. Researchers used a sophisticated cloning procedure to produce bioluminescent bacteria that can be observed using light sen- sitive cameras. This allows scientists to record kinetics and patterns of plant colonization by bacteria. Biotechnological efforts are also well underway to provide alter- native methods to control insects. One such study involves feeding high levels of copper, cobalt, manganese, lead, magnesium, and cacium to a species of insectsknown to teed on vegetable crops. Some vegetables are known to be hyperaccumulators of heavy metals, and by adding the metals at levels toxic to insects, researchers sought to kill the insects without harming the plants. Though re- sults are preliminary, cobalt appears to have the highest toxicity and calcium, magnesium, and manganese the lowest toxicity. Spiroplasms are spiral-shaped, wall-less bacteria that cause corn stunt, citrus stubborn disease, and spiroplasmosis of honeybees. Researchers have developed a cell culture system for one of the primary host plants of the bac- teria, allowing them to maintain cultures, whole cell suspensions, and protoplasts of the virus. Using spiroplasma strains representing in- sect and plant pathogens and I'Pdutin of upiumnic crops, like peauuts, will bofit in the future from bioteciloogy being de-,eboped currently by A uburn reser chers saprophtes, researchers are cur- rently studying growth and physiological responses of plant cells during spiroplasma infections and similar responses of spiroplasmas interacting with plant cells, Rhodobacter sphaeroides, a bacterium essential to photosyn- thesis, and atrazine, a popular her- bicide used on a wide range of crops, do not mix, according to AAES studies. Though it has been known for many years that atrazine kills weeds by inhibiting photosynthesis, the exact mode of action has only recently been determined. Auburn researchers recently found that certain polvpeptides exist in strains of R. sphaerloides resistant to atra- zine. Further exploration of these relationships could unlock the secret of resistance to pesticides by various plants and make pos- sible a whole new era of biological control. Contamination of food and feeds by toxin-producing molds leads to substantial crop losses, especially in corn and peanuts. Auburn resear- chers recently developed an assay using ultraviolet light to measure fluorescence of the aflatoxin pro- duced by the fungus grown on coconut-based medium. While the assay is not practical for field detec- tion of aflatoxin, it has potential as an assay in the study of the genetic basis for aflatoxin synthesis and in the screening of enzymes that selectively degrade the toxin. Such studies may lead to biocontrol in the field for the toxin. Utilizing waste products is an ongoing research objective in several Experiment Station research projects. Researchers recently pro- duced small amounts of ethanol, which can be used for fuel alcohol, from glucosamine, a product of chitin found in waste shell fish. The microorganisms Pachysolen tan- nophilus, a yeast, and Zyrnoomonas mobilis, a bacterium, were grown on the chitin-containing shellfish to produce the alcohol. Using microorganisms to process or- ganic waste into protein and fuel alcohol could be of direct eco- nomic value while also contribut- ing materially to environmental quality via recycling organic waste into valuable products. While laboratory work promises great things for the future, a vigorous field research program at the Experiment Station is geared to more immediately benefit growers and producers. New Viral Disease Found in Alabama Wheat Soil-borne wheat mosaic virus (WSBMV) was found in Autauga County in the spring of 1989, representing the first known record of this disease in the State. This disease is common in the Midwest and is controlled by growing resist- ant soft red winter wheat varieties. Yield of susceptible varieties can be reduced by up to 30 percent. Identi- fication of this disease suggests that selection of WSBMV resistant varie- ties may be important in Alabama. The disease symptoms appear as green to prominent-yellow mosaic leaf symptoms on stunted plants. Diseased wheat plants are typical- ly in lower or poorly drained areas of production. Soil-borne wheat mosaic virus is an interesting disease in that the causal virus is one of onlyafew that are spread in nature by a soil- inhabiting fungus that has a water mobile zoospore. Future research will focus on determining the distribution and importance of soil- borne wheat mosaic virus in the wheat crop in Alabama. New Peanut Fungicides Improve Quality, Profit The effect of fungicides applied to foliage for peanut leafspot and white mold control on fungi in- fecting seeds and on aflatoxin content has been investigated for the past two crop seasons. A single application of the sterol biosynthesis inhibiting fungicides, diniconazole, terbutrazole, and flutolanil, resulted in reduced fungal seed infection, reduced aflatoxin content, and increased quality. Quality increases real- ized through a reduction in seed infection by fungi have resulted in a calculated 5700 per acre net increase. Aflatoxin levels were reduced 11-33 percent. Accent Herbicide Effective For Corn Weed Controti Rhizome johnson- grass is a competitive and persistent weed species for corn produ- cers. It also serves as a host for maize chlorotic dwarf and maize dwart mosaic viruses that cause substantial yield losses annually in Ala- bama. Effective means of controlling this weed pest with herbicides i pr, n'; would improve the applic i e flexibility and effi- zironatelt as '. lti, II t and ben weLll. ciency of corn production in Alabama. Experiments were initiated at four sites in 1988 and 1989 to evaluate rhizome johnsongrass control in corn with Accent her- bicide. Control of broadleaf signalgrass and Texas panicum was also evaluated. Summary of data shows good to excellent control of rhizome johnsongrass, broadleaf signal- grass, and Texas panicum with single and double postemergence applications of Accent herbicide. Controlling a heavy infestation of rhizome johnsongrass and broad- leaf signalgrass population in north- west Alabama in 1989 increased corn yield 60 bushels per acre over the untreated control. Accent also suppressed growth of pigweed and morningglory species. In an experiment at the Plant Breeding Unit at Tallassee, where pigweed, annual morning- glory, large crabgrass, and goose- grass predominated, a double application of Accent combined with a single cultivation increased corn yield 61 bushels per acre over the untreated control. Good to excellent control of Texas panicum was achieved at the Wiregrass Substation in Headland in 1989 with a single application of Accent applied when the grass had four to six leaves. Corn yield was improved 36 bushels per acre over the untreated control. Tomato Spotted Wilt Virus A Threat to Alabama Crops Tomato spotted wilt virus V) is vectored by several f s speciesofthripsandhas Scaused losses of over50 percent in peanut and tomato production in other states. Since its first identification in Alabama in 1986, an- S nual surveys have found the disease to be widespread, but minor in severity, in peanut , producing counties. mg axd The incidence and rowuers he efit the' n- severitybyTSWVwas higher in 1989 than any previous year and yield losses in in- dividual plantings were 10 percent or more, but yield losses generally have been less than 1 percent in individual fields. Control of the thrips vector with recommended insecticide regimes has not resulted in a significant reduction in TSWV spread or inci- dence in peanuts. In 1989 the incidence of TSWV in the varieties Southern Runner and GK-7 was lower than in Florunner, the most commonly grown variety in Alabama. Soil Solarization Effective in Controlling Weeds Soil solarization is the process of using clear polyethylene plastic film during summer fallow periods to capture solar energy and produce soil temperatures high enough to control many annual weeds and kill some disease-causing organ- isms. Research at the Experiment Station indicates that soil solariza- tion in combination with chicken manure effectively controlled morningglory and sicklepod. The solarized chicken manure- soil mix provided 99 percent control of both weed species after a 4-week solarization period. These results were comparable to those where weeds were grown in the absence of chicken manure, but under the same solarization treatments. Un- solarized chicken manure-soil mix provided 41 percent and 49 per- cent control of morningglory and sicklepod, respectively. Temperatures recorded at 6- inch soil depths in solarized and unsolarized plots with chicken manure averaged 25 F and 10 F higher, respectively, than in plots with no chicken manure or solar- ization exposure. Researchers reported that high temperatures are probably responsible for weed control. This method should not be regarded as a universal method of weed control but rather an addi- tional method that, used correctly, has many advantages. It is safe, non-chemical, inexpensive, leaves no phytotoxic (plant injuring) residues, and is simple to apply. Frost-Protecting Sprays Increase Peach Yield Damage or complete loss of Alabama's peach crop due to late spring frost in recent years has been a major problem for growers. Research using growth regulators to delay bloom past the danger of spring frost and enhance cold bud hardiness has produced promising results. The research was initiated in the fall to determine the effect of fall application of gibberellic acid (GA) on delaying bloom of peach trees in the spring as a means of frost protection. In the spring of 1987, trees were in full bloom by March 31 and a frost with temperatures below 25 degrees F occurred on April 1 and April 4 with considerable flower and young fruit damage evident after each frost. No frost injury occurred in the spring of 1989. In the spring of 1987 and 1989, GA treatments of 50 and 100 p.p.m. delayed bloom, whereas the 10 p.p.m. GA treatment bloomed at the same time as the nontreated trees. GA treatments resulted in a greaterpercent of the flowers setting fruit and required more hand thinning. Yield was increased about 30 percent in 1987 and about 40 percent in 1989 by the 10 p.p.m. GA treatment. Yield was increased each year by the 50 and 100 p.p.m. treatment, but not asmuch aswith the 10 p.p.m. treatment. Fruit from the non- treated and the 10 p.p.m. treated trees ripened at the same time, however fruit ripening was delayed by the 50-100 p.p.m. GA treatments. Fruit size was not affected by the different GA treatments. Irrigation Studies Improve Efficiency of Water Use Researchers evaluated several design aspects of small acreage hard hose traveler (SAT's) irrigation systems. One study related to the method of testing travelers for water application uniformity. Two water supply methods, (constant inlet pressureand constant inlet flowrate) had been proposed as part of an ASAE standard. The field study and analysis identified differences that would result from the two test methods. Flowrate, pressure, and resulting depth of application were shown to be different, however, these differences had little effect on measures of application uniformity. In another study, variations in energy requirements were deter- mined for a traveler as its hose was coiled onto the reel. Hosecoiling had the effect of increasing pressure lossesinthehoseand, thus, increas- ing energy requirements for the machine. The major result of this study was the determination of a bend factor which could be used to predict the additional energy losses. During 1989, instrumented run- off plants were established to study the effects of soil compaction and ,eath' r is, an ongolig goal of F periment Station research. reservoir tillage on water utilization. Both natural rainfall and ir- rigation were evalu- ated in replicated plots ot cotton. Irrigation consisted of a LEPA system (Low Energy, IPrecision Application) mounted on a boom traveler. The reservoir tillage concept includes ripping in the furrow (1 foot) and then adding below-surface reser- voirs into the ripped zone. Preliminary re- sults have shown a significant reduction in surface runoff with the utilization of reser- voirs and reduced soil compaction. Wise use of water resources will be even more essential for farners of the future. 7_ RET- I. rhe Southeast has a long ."r " '' tradition of leadership in 14 theforest industry, butre f- ~;cent trends indicate the * 'region will play an even more ' significant role in the next century. Some forecasters are predicting over 60 percent of the nation's forest products will come from /Q < ,, the Southeast by the year 2000. To help meet this demand, forestry y t iresearchers are conducting tests that run the gamut from seed pro- duction to finished products. Forestry research, which includes x / 1 many multi-disciplinary projects, is a classic example of the high tech r new working well with the more down to earth "old.' Computer models are bringing computers c) ithe planning of timber harvest d forest product manufacturing. a " n the other hand, herbicide search to improve seedling . )duction and studies of site '1luation for improving the IL 4 Its of planting provide land- .' ~ners and commercial foresters ~ ! r :-!!! t vital production technology. Herbicide Application Improves r + iSeedling Survival and Growth Kecentstudies indicate erly pine ,edling growth is markedly in- 'L ased when competing grasses ~ a- J herbaceous vegetation are 4 led. Consequently, many forest I indowners are applying herbicides t fr herbaceous weed control soon after planting pine seedlings. Though it is generally assumed that soil moisture is the mechanism which relates reduced herbaceous E 1competition to increased pine :e edling growth, only limited information is available to doc- a ii s iment relationships among 0.".1 " +;f ? heicide applications, nonpine ntagu of the cowiru s timpbeLr ninthe .:t n t rlt century. and researchUICI is gearedi to " ~ ilei~lihelping landowners meeiet tis iresed 12 vegetation, soil moisture, and pine seedling growth. In an Experiment Station test, Oust herbicide was applied to an old abandoned pasture at varied rates in order to obtain a range of her- baceous/grass cover in established study plots. Loblolly pine seedlings were planted and soil moisture levels were monitored for two growing seasons. Lower levels of competing herba- ceous vegetation achieved by herbicide application resulted in increased 2-year pine seedling survival, height growth, and diameter growth. The relationship between nonpine live cover and pine growth was inverse and linear within the range of herbaceous cover achieved. This relationship was clearest and strongest for seed- ling diameter. Broadcast applications of Oust at 4 ounces per acre achieved optimum results from competition cover reduction and pine seedling survival and growth on this site. An 8-ounce rate did not produce improvement over the 4-ounce level. However, the remaining live cover on plots treated with 4 ounces averaged about 35 percent. This suggests that a more effective herbicide applica- tion might have improved pine survival and growth. Soil moisture levels were clearly different between high and low cover levels for the growing season immediately after cover was reduced. The inverse quantitative relationship between the amount of live cover and measured soil moisture was weak. Significant correlations were found between soil moisture and 2-year seedling survival and 1-year seedling diameter growth. It ap- peared that soil moisture utilization by the pine seedlings themselves weakened the measured relation- ships. I oung Pines Can Produce High Quality Timber It has been documented that within a few years, 50 percent of the softwood forests in the South will consist of plantation trees, and by the year 2000, pine plantations will supply more than half of the soft- wood timber. Southern pine plan- tations, particularly loblolly and slash, of ages between 20 and 30 years contain from 30 percent to 60 percent juvenile wood. It is weaker and less stiff, with greater longitudinal shrinkage than mature wood. Plantation trees of these ages also have numerous branches that become knots when logs are sawn, producing lumber of lower grades. An earlier study compared the lumber yield recovery from 20- and 50- year-old slash pine plantations with approximately the same diameter breast height (dbh). Initial spacing of the 20-year-old planta- tion was 12 feet x 12 feet and that of the 50-year-old was 6 feet x 6 feet. The site index for both stands was 105 feet at 50 years. The results indicated that the total lumber volume recovery of the 20-year-old trees was 39 percent of the lumber recovered from the 50-year-old trees. The same study found that the strength properties of lumber from the 20- year-old plantation was only 54 percent the strength of lumber from the 50-year-old trees. All tested lumber from the 50-year-old trees met the required structure standards for the grade. A significant percent- age of the lumber from the 20-year- old trees did not meet the required standards for the grade. A similar Experiment Station study concerned lumber yield recovery and lumber grade recovery from a 27-year-old plan- tation of slash pine in southwest Alabama. The plantation was established on a 6 foot x 6 foot spacing in a location with a site index 90 feet at 50 years. Lumber from the 27-year-old slash plantation was of high quality with exceptional high lumber grade distribution, higher than that of the 50-year-old trees. The grades of all lumber from the 27-year-old stand were 43 percent Number 1, 49 percent Number 2, and 8 percent Number 3. All ob- tained lumber met the required standards for the grade. Results of the Auburn study indicate that relatively young (27-year-old) slash pine plantations with original spacing 6 feet by 6 feet in a good site can produce excep- tionally high quality and valuable lumber. Additional studies are underway by the AAES to investi- gate lumber yield and properties of loblolly plantations of different in- itial spacings, thinnings, site indexes, and ages. New Fungicides, Site Keys to Seedling Disease Control Pine seedling mortality is a major problem on many sites being converted from agronomic crops to forest plantations under the Conser- vation Reserve Program. Agricultural Experiment Station research has demonstrated that mortality on these sites often is associated with decortication of roots and stems by insects, followed by invasion by fungi. Researchers found mortality in such cases is more severe on sites where a plowpan has been developed, and current recommendations call for the plowpan to be broken by a ripper blade prior to planting tree seedlings. One of the disease problems of nursery bed pine seedlings is caused by a binucleate Rhizoctonia sp. Experiment Station research has shown that SN 84364, an experi- mental fungicide, controls the disease. Studies currently are being conducted to define most effective rates and spray schedules. An important observation made during the course of these studies is that seedlings in fall-sown nursery beds are less likely to become diseased than those in spring-sown beds. Nitrogen Nutrition Level Important to Seedling Growth Improving the growth potential of artificially regenerated lands in Alabama is the goal of nursery culture and stand establishment research. Analysis of tree growth and development demonstrates that small increases in seedling size at the time of planting can have a signifi- cant impact on plantation yield. Responses from a number of experiments show that average survival can be increased by 2 to 10 percent and average yields by 1.5 to 5 cords per acre for each millimeter increase in seedling diameter at the time of planting. Current research has focused upon the mechanisms regulating these responses. Experiments at Auburn have demonstrated how the total quantity of needles per seedling, the photosynthetic effi- ciency as affected by nitrogen nutrition, and the development of branches can account for the observed responses. The amount of new root growth, which is essential for initial survival, was shown to be closely related to current photo- synthetic capacity of the seedlings, particularly the amount of foliage, and not the quantity of carbo- hydrate reserves in the tissue as previously thought. Studies also show that the ob- served increases in growth associated with larger diameter seedlings are closely associated with differences in initial foliage biomass and subsequent branch formation, which permits greater light interception. Experiments focusing upon the effects of ni- trogen nutrition on plantation development indicate that im- provements in growth and photo- synthetic efficiency occur even at nutritional levels which are supra-optimal for growth in the nursery. This effect may arise from increased amino acid and protein synthesis and from increased retranslocation of nitrogen from older needles to current growth. Forest Growth Studied From NASA Satellite Auburn researchers working in cooperation with scientists from NASA's Stennis Space Center in Bay St. Louis, Mississippi, used a visible and infrared scanning radiometer to quantify changes in light reflected from the foliage of plants. Both the quantity and quality of light reflected by foliage is thought to be strongly con- trolled by foliar anatomy and the chemical constituents of the foli- age. In the past, scientists have suggested that since various stresses may cause unique changes to the anatomy and chemistry of foliage, reflectance measurements (if precise enough to reveal suffi- cient detail) may be used to monitor the health of our forests from satellites, or to identify specific stresses that are harming the forest. Researchers studied the influence of competition stress on the spectral reflectance of loblolly pine, and found that the degree of competition and the age of the needle strongly influenced the spectral properties. They found strong relationships between several chemical consti- tuents and specific spectral responses for trees grown in the field, but were unable to conclude whether the spectral changes observed were similar to those caused by other stresses, such as air pollutants. Wood Composite I-Beams Affected by Humidity As the cost effectiveness of engineered building products improves, wood composite I-beams are becoming more frequently used in medium- and light- frame wood structural systems. For use as a structural member, such products must be strong enough to take service loads and stiff enough to satisfy the serviceability require- ments during its lifetime. A substantial amount of research has been reported concerning the short-term engineering perform- ance of wood composite I-beams under ambient or dry environ- ments. However, for a better understanding of the engineering performances of wood composite beams under service environments and to improve the efficiency of structural design, information it regard totime-depend- ; i' entflexuralbehavioras , affected by the change I of relative humidity moisture content i needed. To collect thi- information a multi phasewood composit beam research pr gram was launched at the Experiment Station Resultsindicatethat , ,, a significant amount til-0 o,1, total orest (up to 30 percent) of the total creep deflection was attributable to shear deformation in the webs. RFB I- beams performed the best consider- ing shear, while the change in RH af- fectedOSB I-beam performance the greatest. Slip occurred in the flange web joint over a long period of time and was adversely affected by the increase in RH. However, the mag- nitude of the slip was quite small and can probably be neglected. The greatest slip occurred in the OSB I- beams, and the least slip was observ- ed in PLY groups. This information will be useful for the improvement of structural design and methods used to manufacture wood com- posite beams. Surveys Show Research Valuable to Forest Industry A recent Experiment Station study demonstrated the importance of forestry research to the com- petitive economic position of the U.S. industry. The study utilized separate surveys of (1) research institutions (reporting their costs and research success rates), (2) land managers (reporting historical and planned herbicide application rates as well as their stand management strategiesfor the next 40years), and (3) herbicide applicators (reporting what they normally charge for herbicide treatments). Data from these surveys were combined with data describing the impact on pine growth, which can be attributed to herbaceous weed control and forecasts of future stumpage val- ues. These were incorporated into a research evaluation model to fore- cast the future benefits of cur- rent and proposed research and the S rbaceousweedcontrol. Research results in- Sdicated that past and SAS fut ure herbaceous w.ed control research will produce substan- h, t i ai economic benefits. wo different eco- !omic models were u,,d to estimate the alueoftheimpactson pinegrowth. Both were , based on standard for- resourc, est economics method- ologies but differed in theirassump- tions regarding post-treatment management (one assumed stand rotations would be shortened, the other assumed they would remain the same). These methods estimated benefits from research to be between 5111 million and $143 million, depending upon the discount rate assumed. Internal rates of return also were calculated for the ag- gregate research program and were estimated at 14.4 percent and 12.3 percent, respectively, for the two evaluation methods. Lumber Model, SELECT, To Help Furniture Makers Lumber costs make up about 50 percent of the total cost of produc- ing hardwood furniture, and incorrect lumber allocation is a major reason for the relatively high cost. Forestry researchers at Au- burn recently developed SELECT, a computer program that evalu- ates the efficiency of sorting lumber according to grades and sizes. Widespread use of the pro- gram, which can be used on per- sonal computers, could save hard- wood furniture manufacturers 25-30 percent in production cost. Ozone, Acid Rain Effects Begin to Show on Pines An ongoing concern of the forest industry is a much-disputed reduc- tion in growth rate of pine trees in the Southeast. As part of a region- wide test, Auburn researchers are testing the effect of ozone and acid precipitation - two proposed reasons for reduced growth-on loblolly pines. One year results (1988) indicate that low levels of neither ozone nor acid rain had a detrimental effect on tree growth. Trees fumigated at the higher concentrations, however, exhibited a significant loss of needles (early senescence) and had visible symptoms of ozone injury. Symp- toms varied by family, indicating response was under some genetic control. Trees exposed to elevated con- centrations of ozone in 1988 appear to be more sensitive to winter injury in 1989, than those exposed to am- bient or filtered air. These findings are under further investigation. Results from the second growing season (1989) do indicate that trees exposed to the most severe ozone treatment are beginning to exhibit declines in growth, but the analyses are not completed at this time. Trees will be exposed for one more grow- ing season (1990) and then all trees will be harvested. Weed-Free Plots Produce Fastest Growing Trees Controlling weeds early may get trees to harvestable size early, ac- cording to a recent study. These studies, one of 5 years and one 7 years, demonstrate that herbaceous weed control following planting of pine seedlings enhances tree growth more than any other silvicultural treatment. The growth trends from these studies indicate that the forest stands will be of harvestable size 2-3 years before stands without herbaceous weed control. At 5 years of age, two her- baceous weed control growth response studies indicate a posi- tive response in tree height, diameter, and basal area per acre in 5 .... year-old-pines. The Auburn location in- volvedcomparisonsof " , band versus spot . treatments, and the pine response was proportional to the amount of area treated. Basal area increased , over the check by 58 percent and 101 percent for the spot and band treatments, respective- n,tang ly. Complete herba- ceous weed control for 2 years in another loca- tion increased basal area over the check by 144 percent. Density (trees per acre) was im- proved by weed con- trol only at the Auburn location. Rust infection was higherfor theweed control treatments on- Atmlospheri ly at the second loca- threaten for tion, with most of the =tudied by increase due to branch rather than stem infections. After trees reached 7 years old, re sults of three herbaceous weed con- trol studies indicate a positive re- sponse in pine tree height, diameter, basal area per acre, and volume per acre from weed control. The Chesa- peakelocation compared 2 years of broadcast or banded treatments to an untreated check. Loblolly pine volume per acre at age 7 differed be- tween the two weed control treat- ments and was 305 cubic feet per acre (87 percent) and 198 cubic feet per acre (56 percent) greater than the check for the broadcast and band- ed treatments, respectively. A Packaging Corporation loca- tion compared banded and broad- cast weed control applied in the first year, and banded and broad- cast weed control applied in both the first and second years, to an untreated check in slash pines. Vol- ume per acre did not differ by meth- od (band versus broadcast), but in- creased with duration (1 year versus 2 years). First-year weed control in- creased volume per acre at age seven by 130 cubic feet per =:. acre (38 percent), whereas first-year and second-year weed con- trol increased volume .. by 208 cubic feet per acre (62 percent). Two Syears of broadcast S . vegetation control at f t he Champion location . resulted in a slash pine .c -;volume responseof 283 ,: ubicfeet per acre (127 percent) at age seven. S. . Rust incidence in- creased with volume fsponseat the Packag- i Corporation and hampion sites. Stem ust for loblolly pine 4, inged from 3 percent r the check to 11 per- ent forthe2-yearweed ~ntrol treatments. item rust for slash pine ranged from 11 percent for the check to 19 per- cent for the 2-year c piolIutants that est growth are being vegetation control Aubumr researchers treatment. OOD TEHOLG AN NUTRTIO The relationship between the human body and the food we eat has never been so closely scrutin- ized as today. Not only is the safety of our food and fiber supply being closely watched, but so are the pos- sible negative impacts on nutrition. Though not new to the research pro- gram of the Alabama Agricultural Experiment Station, research into the many aspects of this food-body relationship is certain to reach new heights as we push into the 21st century. One of the most pressing food- related problems of the current generation has been the so called "microwave diseases"- the most prominent one being salmonella poisoning. The increase in incidence of these diseases, or reporting of these diseases, has created much concern, especially in the poultry studied by scveral inter-disciplinary industry. Experiment Station research is well along in developing a technique, using a naturally oc- curring, safe compound to combat these disease-causing microbes. Though in initial stages, the research offers high potential for reducing, if not eliminating, surface con- tamination of poultry products by salmonella. The Experiment Station has a broad program of research in the area of human health and nutrition. Still, the most visible and certainly most closely scrutinized program is a wide range of projects, each with one common denominator food. Fish Oils May Reduce Respiratory Diseases Fish oils contain omega-3 fatty acids which have been linked to reduced incidence and severity of heart disease. Consumption of this type of fat may be helpful because it results in a decreased production in the body of compounds called eicosanoids. Certain eicosanoids are known to be increased in diseases such as cardiovascular disease, as well as in asthma, allergies, and cer- tain inflammatory responses. The role of omega-3 fatty acids in respiratory diseases has not been as well-studied as it has in the cardio- vascular system. Scientists in the Agricultural Experiment Station are thus investigating the effects of dietary fish oils on the pulmonary system in the hope that omega-3 fatty acids will be beneficial in treating or preventing diseases of the lung in much the same way that this type of fatty acid reduces cardiovascular disease. Lungs of newborns are par- ticularly vulnerable to disease, thus the researchers used neonatal piglets as a model for the human infant to show that eicosanoids produced by lung tissue are lower in animals fed fish oil than in animals fed either corn oil or coconut oil. Results from these studies have implications for the type that is best for infant formula fed to normal infants as well as those who are undergoing a stress to their lungs. In addition, this research may provide insight into more effective means of treat- ing lung disease, such as asthma, in both children and adults. Fungi Produce Beneficial Omega 3 Fatty Acids World-wide interest in health aspects of fat nutrition has been stimulated in part by the apparent human health benefits of dietary long-chain polyunsaturated fatty acids (PUFA) of the omega-3 type, such as eicosapentaenoic (EPA) and docosahexanenoic (DHA) acids found in the oil of many marine fish species. Research at Auburn has shown that certain primitive fungi produce relatively high amounts of EPA, as well as another PUFA called arachidonic acid (AA), which also has biological activity in mammals. The fungus being used produces about 30 percent fat which contains up to 10 per- cent AA and 12 percent EPA, which is comparable to production by other fungi being developed for such purposes in Japan. The fungus is amenable to scale up bio- mass production using fermenta- tion techniques, and therefore represents a potential commercial source of these fatty acids. Fried Chicken a Good Source of Vitamin B6 Why eat fast food? One Auburn researcher found at least one good reason. Fast food fried chicken is an excellent source of vitamin B and the vitamin is not destroyed during the cooking process. Vitamin Bo is one of the B complex vitamins which is stated in many research reports as being low or limited in the diets of certain populations in the United States. The fact is that many of the foods con- sumed by American consumers have not been evaluated for their content of vitamin B . This lack of information has prompted Auburn researchers to evaluate foods for their content of this vitamin. Meats tend to be the best source of vitamin B which is used in manx different ways in the body. The first step was tio determine a method that would have the sensitivity to detect even the smallest levels of the different forms of vitamin B. The me- od developed used high performance li- quid chromatography (HPLC), which could detect for the six dif- ferent vitamers of B. This method was used to evaluate chicken samples. Fast food fried chicken samples were first evaluated for their Bo content, knowing that if a fried sample of food could be analyzed, the problems associated with other less complex foods would follow. Samples were collected from fast food fried chicken establishments. The chicken pieces were obtained halved, with half remaining raw and the other half prepared according to the individual restaurant standards. The analysis of the matched samples revealed that little of the vitamin was lost during the frying process. This led researchers to conclude that vitamin B is stable at high temperatures for periods up to 20 minutes in a fat media. The breading and batter on the fried chicken may assist in the retention of the vitamin, trapping the juices in the meat and resulting in small losses of the vitamin in processing. Technology Produces New New Beef Products A primary goal of the meat industry, as with any industry, is the production of products with maxi- mal returns. This economic con- cern motivates processors to seek new and innovative technologies for use in the development of new products of higher quality and value, at the lowest cost. Tra- ditionally, beef chucks (27 percent of the beef carcass) have been - rchandised as low-priced roasts and steaks due to the imount of connective issue and the widely .arying palatabil- ty among muscles. Chucks are inexpensive and are attractive as a raw material source for 'he production of value-added'products. Recently, many technological advances have been used in an attempt to develop restructured products which more closely re- d b . t . t be'ef produc ts arecntinoul de-ueloped aid ecauated 13Y Auburn re'searchers. semble intact cuts of meat. One drawback in manufacturing re- structured products with a texture similar to intact muscle is that larger pieces of meat must be used. With the use of larger meat pieces from lower value cuts, there is greater potential for problems with detec- tion of connective tissue. An Experiment Station study was conducted to determine the ef- fectiveness of the trimming of connective tissue from chuck muscles and the reincorporation of the connective tissue after particle size reduction to restructured roast beef. In this study, the trimming of connective tissue from the chuck muscles resulted in a loss of approx- imately 20 percent. Results suggest that the particle size of this connec- tive tissue can be reduced and the connective tissue reincorporated into restructured roast beef pro- ducts without detrimental effects to product sensory quality, thus producing quality products at a reduced cost. Lean Ground Beef Lowers Fat, Calories Over 3 billion pounds of ground beef, which is 44 percent of total fresh beef cuts available, is con- sumed annually in the United States. This ground beef generally con- tains 20-30 percent fat. A large segment of today's consumer population is health conscious and is concerned about dietary fat. These consumers avoid meat products, such as ground beef, with high fat and caloric content. As today's consumers continue to become more health conscious, their demand for lower fat, ground beef will rapidly expand. It is important that low-fat, ground beef is developed and tailored to meet the needs of today's diet conscious consumers. Simple reduction of the fat to 5-10 percent would be the most efficient meth- od of developing low-fat ground beet. However, ground beef with a fat content in this range is gen- erally considered less palatable than ground beef with 20-30 per- cent fat. Therefore, an Experiment Station project was initiated to develop lean ground beef that will be as acceptable to the consumer as current ground beef. Through changes in manufactur- ing processes and addition of food additives, lower fat (10 percent) ground beef products have been developed that possess sensory characteristics that do not differ from the control (20 percent fat). These products contain approxi- mately 40 percent less fat than the control on an "as eaten" basis. Caloric savings obtained from the consumption of these products compared to the control product are between 55 and 60 kcal per 3.5-ounce serving (22-23 percent), with a decrease of 70 calories from fat and an increase of 10 calories from protein. Use of Plant Fat Lowers Cholesterol in Ground Beef Popularity of red meat has decreased over the past several years due to negative publicity about the content of dietary cholesterol, calories, and saturated fats in red meat products. Simple reduction of the fat level in ground beef pro- ducts will not eliminate the problem, since even when the fat level is reduced in ground beef, there is no change in the choles- terol level of the cooked product. Generally, animal fats have been associated with high amounts of cholesterol and saturated fatty acids, whereas plant fats are associated with unsaturated fatty acids. Plant fats are also a good source of saturated fatty acids, however they do not cont v lesterol. Therefore, a study was initiated to evaluate quality attri- butes and overall con- sumer acceptability of ground beef (10 per- cent) produced with beef fat or various sources of hydrogen- ated vegetable fat. Sensory panelists _ overall acceptability ratings forgroundbeef , containing hydrogen- e,'clopr en atedcornfatorpalmfat pa r o did not differ from Alabama-rai t of na. f these prc nwlre conel sed bieef. ground beef containing beef fat. These results indicate that the re- placement of beef fat with hydro- genated plant fat in the manufacture of ground beef is feasible and thus may be an effective method of low- ering the cholesterol content of ground beef. Packaging Problems Remain For Pre-Cooked Roast Beef Currently, the safety of ourfood supply is being questioned. As the market for ready-to-eat food items continues to grow, it is essential that the safety of each of these products is evaluated. One such product is pre- cooked roast beef. The process of slicing and packaging pre-cooked roast beef provides an opportunity for contamination of the product with spoilage and pathogenic organisms. The presence of pathogens in these ready-to-eat items is of great concern. Staphylococcus aureus has been identified as one of the primary causative agents in food- borne disease outbreaks. Experi- ments were designed by food scien- tists to investigate the antimicrobial effects of potassium sorbate, secon- dary heat treatment, and reduced pH of vacuum packaged roast beef slices on the persistence of S. aureus in products stored under refrigerated conditions. Results indicate that secondary heat treatment of vacuum pack- aged, sliced, precooked roast beef lowered S. aureus counts in the roast beef. However, this treat- ment was detrimental to product -nearance The treatment of the -,ast beef with potas- um sorbate or the ,wering of the pH of he roast beef with an icidic solution did not tfectively lower S. Silreus counts. The re- ruction of S. aureus in h nese products is not ifficient, so other post-processing techni- ues must be devel- ,ped to eliminate the p'-athogenfrom thepro- oducts will pIete use of duct while maintaining product quality. resources is an ongoing research emphasis in the Ex- periment Station. Depletion of these resources or depredation from soil, air, or water pollution is a threat to both agricultural and nonagricul- tural areas of the State. Alabama, forexample, ranks se- cond nationally in broiler chicken production, but this production , n , :; ,-archers must also bie eni,ronmetalists, thus the impact of production practices on our water, soil, and air are constantly monitored. level also produces about 2 million tons of chicken litter annually. The poultry industry has played a leadership role in developing alter- native uses of this waste byproduct. Experiment Station research has explored uses of litter ranging from fertilizer for vegetable crops, to pot- ting soil for ornamentals, to feed for livestock. Arecent Experiment Sta- tion survey indicated that litter of average composition has a value of $29 per ton based on its use as a fer- tilizer and $105 per ton if the crude protein, fiber, and mineral content is considered for use as a feed ingre- dient for ruminant animals. Similar studies demonstrated that 35 to 40 percent of the carbohydrates in cer- tain crop residues can be converted to ethanol, lactic acid, and citric acid. Remaining residues are protein enriched by the spent fermentation microorganisms, and these residues have economic value as a source of high quality protein animal feed. Wastewater generated by agri- business is a potential source of environmental pollutant that requires treatment prior to disposal. Auburn researchers are exploring the use of microorganisms to treat wastewater and to convert the nutrients in wastewater to microbial protein which can be recovered and used as feed for animals. Equally innovative work is being done in- volving soil, water, and air factors effecting plant production, plus the effects of the environment on man. Hydrilla Control Possible with Sterile Grass Carp A new exotic plant, called hydrilla, has become a threat to fishing and recreation on Lake Guntersville -Alabama's largest lake. Experiment Station resear- chers recently found that sterile grass carp can help reduce hydrilla, but precautions must be taken. The method of producing sterile grass carp, or white amur, was developed in 1983, making it possi- ble to test these voracious feeders in open waterwithout fear of ongoing environmental damage. As a result, Auburn researchers, working in cooperation with the Tennessee Valley Authority, released 25 juvenile and 10 adult grass carp in- to Guntersville Reservoir. NVIRONMENTAL CONC'E'RNS The juvenile grass carp moved an average of 1.4 miles during summer and reduced movement when water temperatures declined in the fall. These fish tended to move upstream and typically remained in hydrilla- dominated areas. Surprisingly, the adult grass carp moved an average of 20 miles soon after stocking and did not reduce movement when water temperatures began to cool. There is clear evidence that grass carp can adversely affect waterfowl populations by destroying the hydrilla that is preferred nesting and feeding habitat for ducks. By using high stocking rates of juveniles, researchers expect to control the ex- pansion of hydrilla, without significantly destroying areas of the grass used by waterfowl. Cattle Egrets Provide Environmental Insights Cattle egrets provide more than a scenic touch to the pastures they visit. Experiment Station research has shown that they can help con- trol insect populations and also are valuable indicators of environmen- tal pressures. Egrets are colonial waterbirds, birds which nest in or near water in large colonies. By studying egrets and their kin (herons, ibises, gulls, and other birds), scientists have learned more about their lifecycles and have gained insight into the delicate ecosystems of the wetland and shore areas they inhabit. Recent studies of egrets during drought years have shown that the birds are more likely to endure dry weather if abundant pastures are available for feeding. Human har- rassment of egrets severely im- pacted nesting habits and eventu- ally dispersed colonies. These data indicate that egrets are highly susceptible to environmen- tal pressures and suggest that Alabama can keep an attractive, helpful member of its wildlife com- munity by protecting their environ- ment and their privacy. Alabamians Aware of Dangers of Hazardous Materials An Experiment Station study, based on results from three separate mail surveys conducted during the summer and fall of 1988, clearly shows that Alabamians see hazard- ous waste management as a major national problem which presents serious threats to their own communities. A total of 1,200 questionnaires were sent to a random sample of Alabama residents, of which 399 were returned. A further 200 were sent (and 69 returned) to residents of Sumter County, location of the nation's largest hazardous waste landfill. The third group surveyed (200 questionnaires, 127 returned) was the membership of the Ala- bama chapter of the Sierra Club. The surveys show that Alaba- mians are unwilling to trade environmental quality for economic development. All three groups surveyed expressed a strong desire to create more jobs. Most Alabamians said they were willing to pay more for products if this was necessary to promote recycling and waste reduction processes. The surveys also show that Alabamians have considerable faith and confidence in the Environ- mental Protection Agency and the Alabama Department of Environ- mental Management as key agencies in managing hazardous wastes. Alabamians do not believe the private sector should be in- volved in making decisions on where to locate hazardous waste management facilities, although under the present regu- latory structure the ini- tiative in siting new facilities generally is with private firms. Ala- bamians strongly feel that only scientific ex- perts in the field should make decisions regard- ing hazardous waste . management. New Termite a Threat in Parts of Alabama The Formosan sub- terranean termite is one of the most destructive tound only ne termite species world- city of Mobile wide. This termite is an ounu and rec important pest in trop- to homeowner ar Ala , haye ;earch s.d p rS. ical and subtropical areas such as Guam, Taiwan, the Philippines, and Hawaii. In Hawaii, it is estimated that over $60 million is spent annually to control this ter- mite. In the continental United States, the Formosan subterranean termite has been reported primarily in coastal cities from Houston to central Florida, which have ports that service shipping from the Far East and Hawaii. There are no estimates of Formosan termite damage in Alabama, although the termite has been reported in Mobile and Baldwin counties and as far in- land as Auburn. All reports of Formosan sub- terranean termite infestations in the United States, except the one in Auburn, have been near ports or inland waterways. This termite was probably introduced into the Southeast with infested marine cargo in ships returning from the Pacific theater at the end of World War II and the Korean conflict. Subsequent infestations have been caused by transportation of in- fested wood. Because of the economic import- ance of the Formosan subterranean termite, research was undertaken by the Experiment Station to deter- mine the distribution of this pest in Alabama. In addition, physio- logical factors that may reflect the behavior and distribution of this pest were contrasted with those of native eastern subterranean termites. Because the For- mosan subterranean termite is distributed in warm, usually humid regions and frequently : near water, aspects of 'heir temperature rela- I ions were investigated rl and compared to the native eastern subter- ranean termite. Ter- 'nites were heated or )oled at a constant ,te(3.4 Fperminute) determine the bamas port temperatures at which been found they lost the ability to ers is Lee ose a threat move (knockdown). Formosan subterra- mean termites were knocked down at about 3.4 ? hotter than native eastern subterranean termites. This result indicates that Formosan ter- mites can withstand higher temperatures than native termites. Eastern subterranean termites tolerated slightly lower tempera- tures than Formosan termites. Millet Removes Excess N From Poultry Litter Fertilizer One of the biggest problems with using poultry litter as a N source is applying a sufficient quantity of lit- ter to supply the recommended adequate amount of N without applying excessive N that can end up as an environmental pollutant. It is easy to determine the total amount of N in litter, but it is extremely difficult to determine how much of the N will release during the growing season, and how much will be released to the environment. Tests were conducted in the spring of 1989 to determine the amount of N used by millet and wheat. As a result of wet conditions, the wheat was harvested later than normal and the following millet crop was planted approximately 3 weeks later than normal. Despite the late planting date, millet growth was excellent. Millet dry weights on the first cutting (August 30) averaged 2,600, 6,000, and 7,600pounds per acre when inorganic N fertilizer, 4 tons per acre litter, and 8 tons per acre litter, respectively, were applied to the previous wheat crop (the millet was not fertilized). The I aoo ro N remo(ved in the whpat grain plus millet tissue averaged 44 electric constant, nonflammability, and 38 percent of the N applied in and low corrosiveness are also im- the 4- and 8-ton per acre litter portant characteristics for wasp treatments, respectively. The 44 sprays used in and around electrical percent N recovery from the low equipment. rate of litter is as good as can In the Auburn test, 1-minute be expected from inorganic N knockdown ranged from 27 to 100 fertilizer. percent and could be categorized Theresultsof the firstyearof this into three groups: more than 98 test strongly indicate that when percent, 89 to 98 percent, and less poultry litter is the sole N source for than 89 percent. Except for Wasp wheat, total N in the wheat will Killer II, all formulations that had have to exceed three times the N 100 percent knockdown also fertilizer requirements for wheat, damaged some type of plastic. All and that 80 percent of this N formulations having one minute will be left in the environmental knockdown between 98 and 81 per- system after wheatgrain harvest, cent also damaged at least one type However, if a crop such as millet, of plastic. The two formulations that uses large amounts of N, with the lowest knockdown (Black- follows the wheat, a reasonable flag and SCS) did not damage percentage of the excess N can plastics. All formulations gave be removed from the environ- 100 percent kill 5 minutes after mental system. spraying. In further studies, there was no Environmentally Safe Sprays correlation between 1-minute Effective for Stinging Insects knockdown and any of the active Wasps, hornets, and other ingredients in the wasp and hornet stinging insects are a constant pest sprays. There was, however, a and potential health hazard to significant correlation between highly allergic individuals. Though knockdown and the chilling effects most sprays are used by home- of the sprays. The body temperature owners, many service industries, of honey bees used in the test was especially utilities, also must use continuously recorded and sprays sprays to protect their workers, applied. Formulations giving the Finding sprays that are effective, but best knockdown lowered body not damaging to materials in the temperature the most. These environment was the focus of a re- formulations contained Freons, cent Experiment Station study. methelene chloride, and other Electronic and telecommunica- volatile solvents. tions industries must use wasp spray Rapid knockdown of stinging in- formulations that do not damage sects is the most important char- plastic, which is used to form tele- acteristic of wasp and hornet sprays phones, splice cases, and most im- used in electrical and telecommuni- nortantil insulate wires. Hich di- cation industries. Technicians are often faced with wasps infesting suspended aerial equipment. Con- trol also must be immediate since wasp stings might severely affect worker safety, especially when workers are 20-30 feet above the ground. Wasp sprays also must not damage equipment. These studies indicate that rapid knockdown is Mdue to body temperature depression rather than the active ingredients per se and can be accomplished without - .damaging plastics. Auburn researchers monitor land, soil, and air for pollution from a variety of sources. hough Alabamas met ro politan areas are increas- ing in population, a large segment of the State's people stil live in rural areas. Typical of rural families are husbands and wives with both on and off-farm jobs. Many opportunities exist for rural residents. A recent survey, for example, in- dicated that greater flexibility in work schedules among family members is essential for most rural families with both on-farm and off- farm employment. Education was also listed as essential in both ob- taining and enjoying off-farm employment by rural residents. Surveys ot farm families indi- cate that farm crime and financial recovery remain problems for Alabama's farmers. For those seek- ing to develop rural industries, one study indicates that finding a suitable labor force is a problem. Recreation Use Model Developed for State Forests A model of the recreational use of Bankhead National Forest in Alabama was completed. It ac- curately estimates the number of visitors to the forest in a given year. The model also estimates the alloca- metropolitan iaf l , i <,g studied by Aubum rescarchers. tion of visitors among various areas of the forest based on the Recreation Opportunity Spectrum (ROS). In addition, ROS classes of the forest were modified following a newly developed classification system which shows promise for all Eastern national forests. Implications of adoption of the new system and the estimation model are significant. First, the new ROS system will mean more diver- sity of forest use in the East in accordance with the multiple use saw directives of the federal govern- ment. Second, the ability to accurately estimate visitor numbers and their choice of recreational activities will mean better manage- ment of forest resources for timber production as well as recreational use. Money is Main Source of Farm Family Stress The severe financial crisis facing many rural farm families in Alabama has served to focus atten- tion on how families successfully ad- just to both expected and un- expected changes in their lives. Researchers at Auburn have begun to identify sources of stress and types of coping strategies common to these families by conducting research with couples who live on a farm or in a rural area. The life stressors most frequent- ly reported by these couples were those associated with increased strain on the family's money supply and strains arising from a family- owned business or investment. These changes were followed in frequency by strains within the family, including reports of in- creased tension and conflict between family members and increased difficulties associated with being a parent. The third most likely type of stressor experienced by these couples was change asso- ciated with work, including changing jobs, being fired, re- tirement, and job promotions. Couples were least likely to report stressors due to changes in the marriage, death of a family mem- ber or friend, family members moving in or out of the home, illness, or legal problems. With regard to coping strategies, the descriptive data revealed that couples used a variety of coping strategies to deal with difficulties in their lives. Both husbands and wives reported that they typically coped with change by seeking spiritual support and by facing problems head-on with confidence in their ability to handle prob- lems. They reported somewhat less frequently that they relied on relatives, friends, neighbors, and extended family in times of need. They were least likely to seek professional counseling, assistance from community agencies, and information and advice from the family doctor when faced with problems. Alabama Farmers Concerned Over Rural Crime Increase A statewide survey of Alabama farm operators sought to determine opinions about and experiences with crime and vandalism to farms. Questionnaires were completely by 428 farmers who provided informa- tion neverbefore obtained about the extent and impact of farm crime in the agricultural sector. For the first time, data are available on a wide variety of crimes against farm property, including crimes both reported and unreported to law enforcement authorities. Also, uni- que information now exists about perceptions of changes in the number of farm crimes and the seriousness of various types of farm crime in local rural communities. A key finding is the extent to which Alabama farm operators view current trends in farm crime as increasing. Two separate ques- tions asked independently pro- vided highly similar results. About 35 percent believed farm crime had increased in their local community within the past 2 years. Similarly, 40 percent believed that the theft of farm pro- perty in their local com- munity had increased in this same time period. The consisten- cy of these two responses indicates that crimes committed against farm property is a growing concern to Alabama farmers. Additional ques- tions focused on specific aspects of farm crime. About 65 per- cent were worried about the safety of farm property such as machinery, livestock, crops, and supplies. Also, 27percent reported being fear- ful about going to certain areas of their farm after dark. The offenses most frequently considered serious problems were: (1) poaching of fish and wildlife - 42 percent; (2) trespassing on farmland-40 percent; (3) van- dalism by dumping trash on farmland - 36 percent; (4) outsiders planting marijuana on farmland - 30 percent; (5) arson of woods, fields or buildings -29 percent; and (6) burglary of farm buildings - 28 per- cent. Five other types of crime or vandalism offenses were noted as serious by 17 percent or more of these farmers. Lake Weiss a Big Part of County Economy Lake Weiss is known to fisher- men throughout the Southeast as "The Crappie Capital of the World." A recent Experiment Sta- tion survey indicates the lake in northeast Alabama plays a key role in the economy of Cherokee County. A survey was conducted in Cherokee County, Alabama, of the Lake Weiss business community. From a total of nearly 500 businesses, a subsample of 150 businesses was randomly chosen which represented six strata fishery-related isinesses. Of these usinesses, a 50 Stuhze, oub, ate that fishries- related businesses on Lake Weise have a major impact on the economies of the county and area. percent sample of business owners was interviewed. The mean percen- tage of gross business income contributed by tishery activities for the entire community was 21 percent, or S1.3 million dollars. i ;usinesses that earned .,t least 15 percent of their income from t i hery-related activity considered that portion ,t income to be vital to their success. Ninety percent of business owners felt that the fishery would expand in the future. 1$-I~ /p .u -mRLE''TV RP N EOREUS C otton, peanuts, poultry, cattle, and timber remain tradi- tional crops for Alabama producers. However, many new crops have been worked into tra- ditional agriculture in recent years and many others are being tested by Experiment Station researchers for future use. Farm-raised catfish is the most obvious new crop success story, having started asan industry in west Alabama in the mid-1960's. In more recent years blueberries have become significant income pro- ducers in Clay and Escambia counties and production is widespread throughout the State. Experiment Station researchers are constantly evaluating crops and services to find the next one to im- Strawberries, blueberries, and kiwi are pact on the State's economy. among several alternative crops being tested for possible commercial produc- tion in Alabama. Lack of Quality Labor Restricts Apparel Industry Apparel manufacturing is an important component of the rural revitalization potential of Alabama. Alabama's apparel industry with 56,000 workers accounts for approximately 15 percent of the manufacturing employment in the State. The industry's 1988 payroll was over $500 million. Alabama and California are the only states in the United States to show an increase in apparel industry employment between 1978 and 1988. In fact, Alabama had a 24 percent increase in the number of textile and apparel plants. Experiment Station research efforts have concentrated on iden- tifying the factors that can make companies more competitive and obstacles that reduce their competitiveness. The increased emphasis on quick response as a means to meet consumer demands is the primary competitive edge available to American manu- facturers as they strive to compete with imports from low wage level countries. However, quick response requires a flexible work environ- ment in terms of workers, equip- ment, and manufacturing approaches. The primary concern identified in interviews with industry per- sonnel around the State was the unavailability of qualified labor. The need exists for people with apparel-related college degrees to move into mid-level management positions. They are needed to help meet the challenges of change in technology, distribution channels, sources of raw materials, and con- sumer demand. In addition, entry- level operators are needed who have a good work ethic and a willingness to learn and adapt. A number of plant managers indicated that they had enough potential orders to expand pro- duction by as much as 100 percent, but they could not get the workers. Such expansions would not only create jobs for Alabamians, but the wages earned and the products made would generate tax revenues which would benefit all of Alabama's citizens. Tourism Potential Explored in North Alabama Counties A recent Experiment Station study has determined that there are reasonable gains to be made by the residents of both Lawrence and Winston counties in Alabama through the development of a tourism industry. Abundant natural resources in both counties provide a significant base upon which to begin building a tourism program. However, several infra- structural problems in both public and private sectors of each county, as well as a general lack of tourism support facilities, create barriers which must be addressed if any tourism program is to attain its full economic potential. Estimates of tourist-related spending in Lawrence County cur- rently approach approximately $288,500 annually. However, this figure could likely be increased one and a half to two times with the proper development and adquate food and lodging facilities. Likewise, Winston County could realise up to $1,000,000 annually in tourist-related spending when a full- tourism industry with support facilities is in place. Potential economic gains from the development of a coordinated county-wide tourism industry exist in both Lawrence and Winston counties. Whether these gains war- rant the use of implementation and development will depend heavily on the support of the entire county populace, as well as the strength and commitment of the local government. New Plant to Boost Interior Foliage Business Nurserymen, tissue culture labs, and retailers will benefit from increased production of Harbour Dwarf nandina, an easy to grow and maintain foliage plant. Auburn research indicates Harbour Dwarf may lead the way in revitalizing Alabama's interior foliage plant industry. The market for interior foliage plants has declined in terms of the number of producers, production area, and wholesale value since the foliage plant boom of the 1970's. When market demand levels, pro- fits decline dramatically and the market is considered to be mature. In order to rejuvenate this market, new crops must be introduced to stimulate demand. Harbour Dwarf is traditionally used as an exterior landscape plant, however, its delicate looking foliage spreads in a unique horizontal manner, and the plant is quite attractive when grown in a 5-inch pot. Preliminary research indicates that the leaves remain intact and retain their rich green color even after plants have beer in a low light interior environment for sex eral weeks. The attrac tiveness of Harbour Dwarf nandinas, their economical production costs, and their good postproduction lift indicate excellent potentialasaninterior potted plant. The market poten- constantly se tial of Harbour Dwarf crop product nandinas was evalu- fruit shown and profit to ated in four Birming- operations. res eking to s, such as here, to a Alabama ham supermarkets. Consumers purchased the plants at a price and a rate similar to foliage plants of the same size, indicating that Harbour Dwarf nandinas are as acceptable as many other tradi- tional foliage plants for use in interior environments. State's First Kiwi Harvest Offers a Few Surprises The first kiwi research work con- ducted in Alabama was begun in 1986. The research was initiated after considerable interest in kiwi production by Alabama growers was evident and many commercial plantings had been or were being established. The kiwi (Actinidia chinenses Planch) has the potential of a high value crop for Alabama and other Southeastern states. The weather and other environmental consideration in the Southeast are favorable for kiwi production, however, certain cultural and pro- duction practices that are currently utilized must be modified for op- timum plant performance. Current data collected indicate Hayward female variety (the leading female variety in world fruit production) has problems with bud break and initiation and develop- ment of female flowers under the environmental conditions of the Southeast. It has not produced the plant vigor, shoot growth, or fruit yield of Fitzgerald or another seed- ling under evaluation. The male variety Matura bloom period adequately overlapped the first half of the female bloom period as de- sired. However, the 1 Tomuri male variety bloomed after the fe- male varieties and did , not overlap at the last y half of the female bloom period as de- sired and reported in the literature. Fruit of the Fitzgerald has the same quality, earchers are develop new butisnotaslargeasthe the kiwi Hayward. However, it dd flexibility should be of acceptable farm commercial size. -V. R- - A RD F NANCIAL REPORT Alabama Agricultural Experiment Station Report of Revenue For The Fiscal Year Ending September 30, 1989 APPROPRIATED FUNDS $15,694,805 51.0% S 1,691,151 5.5% S 3,993,691 13.0% State State Paid Employee Benefits Federal CONTRACTS AND GRANTS Federal State Private lot :-b~ 4~r en Hajek (top), professor of agronomy and soils, was recently presented the annual Director's Senior Research Award. The 510,000 award is sponsored by the Experiment Station and can be used by the researcher to enhance his/her research efforts. Ray Henry (above), associate professor of zoology and wildlife sciences, was presented the Direc- 'I tor's Research Award for Associate and Assistant Professor rank. it is also a $10,000 award. The Director's Research Award has become one of the most coveted and competitive awards for Experi- ment Station scientists. Initiated in 1981 to recognize special lifetime achievements, researchers are nominated for the award by their peers and winners are selected by a committee of their peers. AUXILIARY REVENUES Sale of Research Products Diagnostic Services & Fees Interest and Royalties Indirect and Other Cost Recovery S 1,856,035 645,781 2,409,561 $ 4,911,377 16.0% S 3,471,971 251,583 288,837 465,861 $ 4,478,252 14.6% TOTAL REVENUES $30,769,276 100.0% a v