2011 National Cotton Fusarium Wilt Report January 2012 Agronomy And SoilS depArtment SerieS no. 319 AlAbAmA AgriculturAl experiment StAtion WilliAm bAtchelor, director Auburn univerSity Auburn, Al 36849 thiS report iS A joint contribution betWeen uSdA-ArS, crop Science reSeArch lAborAtory, miSSiSSippi StAte univerSity, miSSiSSippi, And the AlAbAmA AgriculturAl experiment StAtion, Auburn univerSity, AlAbAmA Information contained herein is available to all persons regardless of race, color, sex, or national origin 2011 NATIONAL COTTON FUSARIUM WILT REPORT 3 National Cotton Fusarium Wilt Report ......................................................................... 4 National Fusarium Wilt Trial Figure 1: Field plot layout ....................................................................................................... 6 Table 1: Least squares estimates of the average percent wilted plants ................................... 7 Table of Contents Commercial Cotton Wilt Trial Table 2: Least squares estimates of the average percent wilted plants .................................. 14 Table 3: Least squares estimate of root knot number ............................................................. 15 Table 4: Least squares estimate of root knot egg number ..................................................... 16 Table 5: Least squares estimates of seed cotton yield ............................................................ 17 2011 NATIONAL COTTON FUSARIUM WILT REPORT Kathryn M. Glass1, Katheryn Lawrence2, and Edzard van Santen3, Advisor III, Natl Res. Programs and Professor, respectively, Dept. of Agronomy and Soils, Auburn University, AL 36849 2, Associate Professor, Dept. of Entomology and Plant Pathology, Auburn University, 36849 1,3 Cotton cultivars and elite breeding lines submitted by 20 cooperators were evaluated for Fusarium wilt resistance under field conditions at the E. V. Smith Research Center, Plant Breeding Unit, Tallassee, Alabama. These entries were grown on an Independence loamy fine sand highly infested with the Fusarium wilt fungus (Fusarium oxysporum) Schlect. f. vasinfectum [Atk.] (Snyd. & Hans.) and southern root-knot nematodes (Meloidogyne incognita.). In 2008, a soil analysis for nematodes revealed that southern root-knot (Meloidogyne incognita) was the predominant nematode species in the test plots. The North Fusarium wilt field plot contains a population of M. incognita that ranges from 155 to 1546 J2 per 150 cc of soil with a mean of 711 J2. The populations in the South Fusarium wilt field are lower with a range from 77 to 1004 J2 per 150 cc of soil and a mean population of 378. Other nematode genera present are stubby root (Trichodorus sp.) and stunt (Tylenchorhynchus sp.). Root-knot nematodes, however, appear to be causing the major damage to cotton in the Fusarium Wilt Test as indicated by the high galling indices found on the roots of all cotton lines. The root-knot nematode population throughout the entire test area, i.e., even the areas with the lowest root-knot nematode populations, is more than sufficient to cause a high incidence of Fusarium wilt. Cotton lines submitted to the Commercial Fusarium Wilt Trial were examined to determine their response to both pathogens the root-knot nematode (Meloidogyne incognita race 3) and Fusarium oxysporium f. sp vasinfectum . The field has a long history of root-knot nematode infestation. Plots consisted of 1 row, 20 ft long, with 36 in row spacing and were planted in a randomized complete block design with four replications. All plots were maintained throughout the season using standard herbicide, insecticide, and fertility production practices as recommended by the Alabama Cooperative Extension System. Three plants per plot were removed on July 27. The fresh root weights were recorded and nematodes were extracted from the root system by shaking in 0.6% NaOCl counted under the inverted microscope. Fusarium was aseptically isolated on acidified potato dextrose media from systematic plants removed July. Data was statistically analyzed using Generalized Linear Mixed Models procedures as implemented in SAS® PROC GLIMMIX with a negative binomial distribution function for count variables. Percentage data converted to decimal fractions were treated as a pseudo-binomial, whereas seed cotton yield data in the commercial test were analyzed using a normal distribution function. Dunnett’s P-values were calculated to compare entries to check cultivars. Monthly average maximum temperatures from planting in April through harvest in September were 80.7, 84.9, 95.8, 92.9, 95.6, and 85.2°F with average minimum temperatures of 52.5, 57.4, 69.3, 72.4, 70.2, and 62.7°F, respectively. Rainfall accumulation for each month was 1.91, 2.22, 2.24, 8.02, 0.64, and 4.9 in with a total of 19.93 in over the entire season. 2011 NATIONAL COTTON FUSARIUM WILT REPORT 5 The 2011 season, environmentally, experienced prolonged periods of drought which reduced the incidence and severity of the root-knot nematode and Fusarium wilt pathogens. The numbers of root-knot nematodes increase in all the cotton samples submitted at 45 days after planting (DAP) and at harvest. The standard susceptible cotton, Rowden, averaged 241 root-knot juveniles and eggs per gram of root while the M-315 resistant cotton supported 66 root-knot juveniles and eggs per gram of root at 45 DAP. Nematode juveniles and eggs extracted from the root systems for all the submission ranged from a high of 1315 in FM 1740 B2F to a low of 18 in PHY 367 WRF. The reproductive potential of the nematode varied widely from highly susceptible (FM 1740 B2F, STV 5458 B2RF, and PHY 375 WRF) to low susceptibilities (PHY 367 WRF, STV 5288 B2F, and CG 3787 B2RF) depending on the cotton submission. Re-isolation of the Fusarium wilt fungus Fusarium oxysporum f. sp. vasinfectum was conducted to confirm the presence of the disease pathogen. The fungal pathogen was not found in the resistant M -315 cotton but was readily isolated from Rowden. Over all the cotton submissions planted in 2011, 68.42% were colonized by Fusarium oxysporum f. sp. vasinfectum. Entries were planted in single 20-foot rows on 36-inch centers, separated by 6-foot alleys. Four replications of the test entries and checks were evaluated in a randomized complete block design with a split plot restriction on randomization. The set of eight test cultivars submitted by a cooperator was always evaluated as a group together with two control plots within each replicate. Both susceptible (Rowden) and resistant (M-315) cultivars were included as check subplots in the two center rows of each main plot (Fig. 1). An industry-sponsored cotton test was planted adjacent to the National Fusarium Wilt Trial (NFWT) to compare 17 commercially available cotton cultivars to Rowden and M-315 for wilt response, root-knot nematode reaction, and yield. Trial maintenance was similiar to the NFWT. Results are presented in Tables 2-5, starting on page 14 of this report. Initial plant counts were made on June 21. Wilted plants were counted and removed on July 7, July 28, and August 18. The remaining live plants were counted and recorded on August 30. Total percent wilted plants were then determined and mean wilting for a given entry calculated. The average % wilted plants for the susceptible check Rowden was 11%, with a range from 0 to 83% on an individual plot basis (Fig. 1). Wilt development was low but quite uniform in all blocks with rep averages ranging from 3.5 to 3.8%. The resistant check M-315 had an average of 2% wilted plants, with a range of 0 to 9%. Critical evaluations of breeding lines should be made relative to the Rowden check listed at the bottom of each group. 6 ALABAMA AGRICULTURAL EXPERIMENT STATION Fig. 1. Field plot layout and % wilt for control plot of Rowden (susceptible) and M-315 (resistant). Distances (ft) from the NE corner of the trial are given in the left hand column and the bottom row. NS 338 83 1 1 1 4 17 3 3 8 2 Rowden M-315 19 1 Rowden M-315 5 1 M-315 Rowden 2 8 M-315 Rowden 2 3 Rowden M-315 1 1 312 Rowden M-315 58 2 Rowden M-315 2 1 M-315 Rowden 1 1 M-315 Rowden 4 2 M-315 Rowden 7 2 286 Rowden M-315 2 9 Rowden M-315 5 14 Rowden M-315 1 5 M-315 Rowden 6 3 Rowden M-315 2 1 260 M-315 Rowden 8 1 M-315 Rowden 0 10 M-315 Rowden 1 3 Rowden M-315 2 21 Rowden M-315 14 1 234 Rowden M-315 1 4 Rowden M-315 3 1 M-315 Rowden 2 6 M-315 Rowden 3 8 Rowden M-315 21 1 208 M-315 Rowden 26 8 rows 3 8 rows Rowden M-315 13 2 8 rows Rowden M-315 2 28 8 rows Rowden M-315 3 7 8 rows Rowden M-315 1 2 8 rows 182 Rowden M-315 1 2 Rowden M-315 1 14 M-315 Rowden 1 2 Rowden M-315 1 1 Rowden M-315 1 4 156 M-315 Rowden 29 1 M-315 Rowden 7 1 Rowden M-315 3 9 M-315 Rowden 1 5 M-315 Rowden 21 8 130 Rowden M-315 14 1 Rowden M-315 4 1 Rowden M-315 1 3 M-315 Rowden 7 2 Rowden M-315 1 10 104 Rowden M-315 1 16 Rowden M-315 10 2 M-315 Rowden 2 1 Rowden M-315 7 1 M-315 Rowden 1 9 78 M-315 Rowden 36 1 Rowden M-315 1 11 Rowden M-315 1 23 Rowden M-315 1 15 M-315 Rowden 3 16 52 Rowden M-315 16 1 M-315 Rowden 1 8 M-315 Rowden 1 3 M-315 Rowden 1 5 M-315 Rowden 1 12 26 Rowden M-315 7 16 M-315 Rowden 1 16 M-315 Rowden 16 6 M-315 Rowden 1 3 M-315 Rowden 1 29 0 EW M-315 Rowden 24 M-315 Rowden 54 Rowden M-315 84 M-315 Rowden 114 M-315 Rowden 144 2011 NATIONAL COTTON FUSARIUM WILT REPORT 7 Table 1. Percent wilted plants for entries and check in each replicate, least squares estimate of the average, P-value based on Dunnett’s versus the resistant check M-315, and initial average number of plants per plot. Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. no. Avg.P-value of plants 31 39 34 42 46 37 39 38 45 18 53 78 69 57 44 43 63 69 49 26 48 40 60 53 56 59 61 52 26 29 O. Lloyd May, Delta and Pine Land Co., 381 William Gibbs Rd, Tifton, GA 31794 101 LM-1 7 5 3 1 3 0.473 102 LM-2 7 2 1 2 3 0.507 103 LM-3 1 1 1 1 1 0.900 104 LM-4 2 2 3 1 2 0.770 105 LM-5 0 14 1 0 3 0.625 106 LM-6 1 3 15 9 5 0.257 107 LM-7 26 6 3 1 8 0.087 108 LM-8 0 6 1 3 2 0.703 Rowden 16 9 3 3 8 0.097 M-315 7 1 8 2 3 Dawn Fraser, Monsanto Company, 741 Coker Farm Road, Hartsville, SC 29550 201 DF-1 0 1 0 1 0 0.700 202 DF-2 0 2 0 3 1 0.353 203 DF-3 1 0 0 0 1 0.637 204 DF-4 2 0 2 0 1 0.453 205 DF-5 0 5 1 1 1 0.350 206 DF-6 2 1 12 2 4 0.106 207 DF-7 0 2 0 0 1 0.607 208 DF-8 11 0 0 0 3 0.116 Rowden 14 3 14 1 8 0.022 M-315 1 1 1 1 1 Darren Jones, Monsanto Company, 3410 N. Elm Avenue, Lubbock, TX 79403 301 DJ-1 0 5 1 1 2 302 DJ-2 0 2 5 5 2 303 DJ-3 2 0 0 1 1 304 DJ-4 0 12 1 0 3 305 DJ-5 0 9 2 2 3 306 DJ-6 0 3 2 1 1 307 DJ-7 0 3 0 1 1 308 DJ-8 0 1 5 5 2 Rowden 1 15 2 8 6 M-315 2 1 6 2 2 † 0.569 0.398 0.869 0.358 0.367 0.718 0.773 0.397 0.066 The number listed in the average column is the estimate of the average wilt percentage based on a generalized linear mixed model with the binomial distribution for fixed effects. This estimate will generally be close, but may or may not be identical to the arithmetic average obtained by averaging the numbers in the columns representing the 4 reps. 8 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 1. continued. Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. no. Avg.P-value of plants 66 55 51 57 57 53 63 52 51 26 56 59 59 59 36 52 61 48 40 16 Dave Albers, Monsanto Company, 800 N Lindbergh Blvd., St. Louis, MO 63167 401 DA-1 1 4 0 0 1 0.887 402 DA-2 0 0 0 0 0 0.957 403 DA-3 0 0 1 1 0 0.954 404 DA-4 0 0 1 0 0 0.962 405 DA-5 0 1 0 5 2 0.833 406 DA-6 0 2 1 0 1 0.933 407 DA-7 0 2 0 0 1 0.950 408 DA-8 5 5 2 3 4 0.406 Rowden 16 3 1 2 6 0.156 M-315 6 9 1 1 3 Richard Sheetz, Monsanto Company, 3410 N. Elm Avenue, Lubbock, TX 79403 501 RS-1 0 2 0 1 0 0.658 502 RS-2 1 0 2 0 1 0.612 503 RS-3 19 2 0 0 3 0.070 504 RS-4 2 0 3 0 1 0.451 505 RS-5 75 3 1 3 15 0.002 506 RS-6 3 2 2 3 1 0.312 507 RS-7 3 1 0 0 1 0.554 508 RS-8 7 2 1 2 2 0.213 Rowden 16 7 3 2 5 0.039 M-315 1 2 1 4 1 Daryl Bowman, North Carolina State University, 3709 Hillsborough Street, Raleigh, NC 27607 601NC1101 1 1 11 1 2 0.576 27 Thomas Brooks, Americot Inc., 5017 122nd Street, Lubbock, TX 602 NG-1 0 0 1 602 NG-1 0 0 1 604 NG-3 0 0 4 605 NG-4 0 0 0 606 NG-5 2 0 0 607 NG-6 0 0 0 608 NG-7 0 0 0 Rowden 5 2 1 M-315 1 1 2 79424 1 1 0 0 3 0 0 14 5 1 1 1 0 1 0 0 5 2 0.814 0.814 0.705 0.910 0.658 0.904 0.922 0.104 78 78 77 70 64 68 78 38 23 2011 NATIONAL COTTON FUSARIUM WILT REPORT 9 Table 1. continued. Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. no. Avg.P-value of plants Mustafa McPherson, PhytoGen Seed Co., LLC, 118 Kennedy Flat Road, Leland, MS 38756 701 PHY- MM1 0 3 0 0 1 0.898 64 702 PHY- MM2 0 11 0 2 3 0.500 60 703 PHY- MM3 ‡ 704 PHY- MM4 0 1 1 1 0 0.928 47 705 PHY- MM5 1 0 1 1 1 0.936 59 706 PHY- MM6 0 2 0 1 1 0.933 57 707 PHY- MM7 ‡ 708 PHY- MM8 2 1 2 1 1 0.860 49 Rowden 5 36 13 4 14 0.006 45 M-315 1 1 2 17 3 19 Fred Bourland, University of Arkansas, P.O. Box 48, Keiser, AR 801 FB-1 0 1 3 802 FB-2 0 1 2 803 FB-3 1 1 6 804 FB-4 2 1 2 805 FB-5 1 1 3 806 FB-6 5 75 100 807 FB-7 1 1 6 808 FB-8 2 1 2 Rowden 11 10 2 M-315 1 2 1 72351 2 2 2 14 14 25 1 2 19 1 1 1 1 3 1 30 1 1 12 1 0.558 0.551 0.552 0.170 0.561 0.001 0.669 0.453 0.014 28 28 20 32 24 22 29 32 37 21 64 77 55 67 76 65 19 35 44 24 Brent Styles, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 27893 901 MR-1 0 0 1 0 0 902 MR-2 0 0 0 0 0 903 MR-3 0 0 4 1 1 904 MR-4 0 0 2 0 1 905 MR-5 2 3 3 0 2 906 MR-6 0 0 0 1 0 907 FS-1 1 2 3 1 2 908 FS-2 0 10 2 3 5 Rowden 5 11 8 3 7 M-315 1 1 1 3 1 ‡ 0.739 0.783 0.414 0.618 0.209 0.752 0.371 0.075 0.032 These entries could not be evaluated because fewer than 7 seedlings emerged. 10 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 1. continued. Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. no. Avg.P-value of plants 0.971 0.980 0.988 0.984 0.988 0.288 0.591 0.917 0.424 50 64 84 71 80 33 46 45 52 20 56 33 32 38 43 39 45 40 37 27 21 33 28 45 33 32 19 14 32 16 Brent Styles, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 27893 1001 BS-1 1 1 0 0 0 1002 BS-2 0 0 0 1 0 1003 BS-3 0 0 0 0 0 1004 BS-4 0 0 0 0 0 1005 BS-5 0 0 0 0 0 1006 BS-6 0 1 10 32 6 1007 FS-3 10 1 0 1 4 1008 FS-4 1 1 5 1 1 Rowden 6 5 0 9 5 M-315 4 1 10 2 4 Brent Styles, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 27893 1101 LB-1 0 0 1 0 0 1102 LB-2 1 1 2 1 1 1103 LB-3 9 15 3 3 9 1104 LB-4 1 0 1 1 1 1105 LB-5 0 6 1 1 3 1106 LB-6 0 1 2 1 1 1107 FS-5 0 2 1 1 1 1108 AM-1 0 0 1 1 1 Rowden 8 16 14 8 11 M-315 1 3 1 2 2 Brent Styles, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 27893 1201 MS-1 0 2 8 6 1 1202 MS-2 1 1 0 3 1 1203 MS-3 2 1 2 2 2 1204 MS-4 0 0 6 1 1 1205 MS-5 1 1 2 3 1 1206 MS-6 2 3 8 6 3 0.836 0.717 0.020 0.775 0.281 0.750 0.687 0.774 0.008 0.620 0.642 0.461 0.570 0.523 0.300 David Weaver, Auburn University, 201 Funchess Hall, Auburn, AL 36849-5415 1207 AU 3095 6 1 2 3 4 0.290 1208 AU 3202 20 1 5 2 5 0.244 Rowden 16 23 26 7 19 0.126 M-315 1 1 3 2 1 2011 NATIONAL COTTON FUSARIUM WILT REPORT 11 Table 1. continued. Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. no. Avg.P-value of plants 2 3 2 2 1 2 0 2 24 0 4 2 3 3 1 3 1 1 23 1 0.100 0.060 0.113 0.104 0.387 0.083 0.651 0.142 0.000 52 19 43 29 64 54 59 40 48 32 45 47 52 44 58 53 46 29 46 21 49 52 63 44 48 46 47 40 44 25 Charlie Cook, All-Tex Seed, 356 Hosek Road, Victoria, TX 77905 1301 CC-01 0 1 4 6 1302 CC-02 3 1 50 10 1303 CC-03 0 1 10 1 1304 CC-04 1 10 1 1 1305 CC-05 1 0 0 2 1306 CC-06 2 2 5 2 1307 CC-07 0 0 0 0 1308 CC-08 2 1 2 3 Rowden 18 4 4 83 M-315 1 1 1 1 Cody Poage, All-Tex Seed, P.O. Box 1057, Levelland, TX 79336 1401 CP-01 0 12 2 3 1402 CP-02 0 2 2 4 1403 CP-03 2 10 0 1 1404 CP-04 8 0 3 1 1405 CP-05 0 2 0 1 1406 CP-06 0 8 1 3 1407 CP-07 0 0 2 5 1408 CP-08 2 1 1 1 Rowden 29 16 28 6 M-315 1 1 2 3 0.230 0.320 0.291 0.265 0.571 0.292 0.432 0.417 0.107 Tim Dabbert, Monsanto Company, 749 W. Ash Avenue, Casa Grande, AZ 85193 1501 TD-1 2 0 7 2 2 0.252 1502 TD-2 1 1 3 2 2 0.356 1503 TD-3 1 0 0 2 1 0.530 1504 TD-4 3 3 1 2 2 0.226 1505 TD-5 3 3 7 0 3 0.188 1506 TD-6 2 3 1 2 2 0.231 1507 TD-7 2 3 3 1 2 0.340 1508 TD-8 0 6 6 0 2 0.247 Rowden 29 7 21 5 15 0.004 M-315 1 1 2 1 1 12 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 1. continued. Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. no. Avg.P-value of plants 46 62 52 46 57 58 71 66 52 24 39 67 44 41 22 29 30 25 45 20 31 23 28 25 16 24 24 26 29 20 Joe Johnson, PhytoGen Seed Co., LLC, 118 Kennedy Flat Road, Leland, MS 38756 1601 PHY-JJ1 5 1 0 1 2 0.293 1602 PHY-JJ2 0 0 1 0 1 0.622 1603 PHY-JJ3 3 1 0 1 1 0.431 1604 PHY-JJ4 2 1 3 1 1 0.418 1605 PHY-JJ5 0 0 1 0 0 0.730 1606 PHY-JJ6 0 0 5 2 2 0.296 1607 PHY-JJ7 0 0 1 0 1 0.663 1608 PHY-JJ8 0 0 0 1 1 0.639 Rowden 16 7 4 2 7 0.033 M-315 1 1 1 1 1 Bryan Shook, All-Tex Seed, 356 Hosek Road, Victoria, TX 77905 1701 BS-01 3 1 1 1 1702 BS-02 0 1 0 0 1703 BS-03 1 5 1 0 1704 BS-04 3 5 7 14 1705 BS-05 6 1 3 10 1706 BS-06 3 1 1 1 1707 BS-07 6 5 5 1 1708 BS-08 3 1 1 1 Rowden 29 29 3 58 M-315 1 1 7 2 2 1 1 6 5 1 3 2 30 2 0.591 0.879 0.687 0.105 0.183 0.649 0.351 0.592 0.000 Nilesh Dighe, Monsanto Company, 3410 N. Elm Avenue, Lubbock, TX 79403 1801 ND-1 4 3 3 4 2 1802 ND-2 2 2 1 33 1 1803 ND-3 2 2 2 6 1 1804 ND-4 13 6 1 2 4 1805 ND-5 5 8 2 100 3 1806 ND-6 13 5 1 2 5 1807 ND-7 3 8 1 3 2 1808 ND-8 15 4 2 1 5 Rowden 12 10 1 1 5 M-315 1 1 2 1 1 0.236 0.484 0.413 0.087 0.165 0.078 0.217 0.068 0.068 2011 NATIONAL COTTON FUSARIUM WILT REPORT 13 Table 1. continued. Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 79424 0 0 0 0 1 0 0 0 5 1 79424 1 0 1 0 0 Avg. no. Avg.P-value of plants 1 1 1 0 0 0 0 1 12 2 1 0 1 0 2 0.768 0.873 0.869 0.917 0.912 0.911 0.908 0.782 0.013 62 69 68 69 66 68 68 67 32 23 62 72 60 73 59 12 21 56 43 25 Thomas Brooks, Americot Inc., 5017 122nd Street, Lubbock, TX 1901 NG-8 1 0 2 1902 NG-9 0 0 1 1903 NG-10 0 1 0 1904 NG-11 0 0 0 1905 NG-12 0 1 0 1906 NG-13 0 0 0 1907 NG-14 0 1 0 1908 NG-15 0 3 0 Rowden 3 21 21 M-315 1 8 1 Thomas Brooks, Americot Inc., 5017 122nd Street, Lubbock, TX 2001 NG-16 0 1 0 2002 NG-17 0 0 0 2003 NG-18 1 0 0 2004 NG-19 0 0 0 2005 NG-20 1 0 6 0.615 0.785 0.619 0.785 0.298 David Weaver, Auburn University, 201 Funchess Hall, Auburn, AL 36849-5415 2006 AU 3223 1 3 2 6 2 0.387 2007 AU 3111 2 27 1 1 11 0.015 Kathryn M Glass, Auburn University, 201 Funchess Hall, Auburn, AL 36849-5415 2008 PHY 499WRF 3 0 1 2 2 0.364 Rowden 3 14 3 1 4 0.112 M-315 1 1 1 1 1 14 Commercial Cotton Wilt Trial 95% Confidence Limit Cultivar AM 1550 B2RF CG 3787 B2RF DG 2570 B2RF DP 0912 B2RF DP 0949 B2RF DP 1028 B2RF DP 1050 B2RF DP 10R052B2R2 DP 1137 B2RF FM 1740 B2F PHY 367 WRF PHY 375 WRF PHY 485 WRF PHY 565 WRF STV 4288 B2F STV 5288 B2F STV 5458 B2RF M-315 Rowden Avg 0 1 1 1 1 3 1 3 2 1 1 1 0 2 1 0 0 2 2 Lower 0 0 0 0 0 1 0 1 0 0 0 0 0 1 0 0 0 1 1 Upper 5 5 3 3 8 11 5 8 11 3 5 4 7 8 4 4 4 8 6 Rowden 0.988 1.000 0.993 0.995 1.000 1.000 1.000 1.000 1.000 0.998 1.000 0.984 0.992 1.000 0.997 0.975 0.932 1.000 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 2. Least squares estimates of the average percent wilted plants for entries and checks, confidence intervals, and P-values based on Dunnett’s versus the susceptible check Rowden and the resistant check Dunnett’s P vs. M315 0.957 0.995 0.960 0.969 0.998 1.000 0.997 1.000 1.000 0.981 0.999 0.943 0.972 1.000 0.977 0.927 0.852 1.000 2011 NATIONAL COTTON FUSARIUM WILT REPORT 15 Table 3. Least squares estimate of root knot number (counts per 150 cc) for entries and checks, confidence intervals, and P-values based on Dunnett’s versus the susceptible check Rowden and the resistant check M-315. Samples were collected at harvest. 95% Confidence Limit Cultivar AM 1550 B2RF CG 3787 B2RF DG 2570 B2RF DP 0912 B2RF DP 0949 B2RF DP 1028 B2RF DP 1050 B2RF DP 10R052B2R2 DP 1137 B2RF FM 1740 B2F PHY 367 WRF PHY 375 WRF PHY 485 WRF PHY 565 WRF STV 4288 B2F STV 5288 B2F STV 5458 B2RF M-315 Rowden Avg 194 528 205 316 281 544 36 19 185 297 147 431 227 427 26 24 344 14 270 Lower 18 50 26 39 27 32 3 2 11 37 14 54 13 40 2 2 33 2 34 Upper 2047 5583 1648 2536 2969 9278 379 205 3161 2383 1553 3466 3869 4517 278 259 3642 111 2168 . Dunnett’s P vs. Rowden 1.000 1.000 1.000 1.000 1.000 1.000 0.843 0.539 1.000 1.000 1.000 1.000 1.000 1.000 0.696 0.659 1.000 0.278. 0.278 M315 0.537 0.158 0.397 0.222 0.362 0.282 1.000 1.000 0.728 0.244 0.681 0.137 0.637 0.213 1.000 1.000 0.283 16 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 4. Least squares estimate of root knot egg number (counts per g of root fresh weight) for entries and checks, confidence intervals, and P-values based on Dunnett’s versus the susceptible check Rowden and the resistant check M-315. Samples collected at the 6-8 leaves stage. 95% Confidence Limit Cultivar AM 1550 B2RF CG 3787 B2RF DG 2570 B2RF DP 0912 B2RF DP 0949 B2RF DP 1028 B2RF DP 1050 B2RF DP 10R052B2R2 DP 1137 B2RF FM 1740 B2F PHY 367 WRF PHY 375 WRF PHY 485 WRF PHY 565 WRF STV 4288 B2F STV 5288 B2F STV 5458 B2RF M-315 Rowden Avg 75 57 161 295 273 125 89 85 119 1315 18 1019 81 383 161 37 54 66 241 Lower 8 6 23 42 28 8 9 9 7 187 2 145 5 40 17 4 6 9 34 Upper 726 548 1135 2072 2636 2051 860 818 1951 9249 178 7166 1323 3703 1553 354 524 463 1693 Dunnett’s P vs. Rowden 0.999 0.989 1.000 1.000 1.000 1.000 1.000 1.000 1.000 0.922 0.609 0.977 1.000 1.000 1.000 0.912 0.985 0.991 0.991 M315 1.000 1.000 1.000 0.969 0.990 1.000 1.000 1.000 1.000 0.300 0.997 0.412 1.000 0.944 1.000 1.000 1.000 2011 NATIONAL COTTON FUSARIUM WILT REPORT 17 Table 5. Least squares estimate of seed cotton yield (lbs per acre) for entries and checks, confidence intervals, and P-values based on Dunnett’s versus the susceptible check Rowden and the resistant check M-315. Dunnett’s P vs. Cultivar AM 1550 B2RF CG 3787 B2RF DG 2570 B2RF DP 0912 B2RF DP 0949 B2RF DP 1028 B2RF DP 1050 B2RF DP 10R052B2R2 DP 1137 B2RF FM 1740 B2F PHY 367 WRF PHY 375 WRF PHY 485 WRF PHY 565 WRF STV 4288 B2F STV 5288 B2F STV 5458 B2RF M-315 Rowden Yield [lbs/acre] 3714 4225 3249 3586 2964 4100 3240 3058 3563 3242 2915 3579 4050 2954 4672 3765 4203 1742 2443 SE 377 377 324 324 377 465 377 377 465 324 377 324 465 377 377 377 377 324 324 Rowden 0.146 0.012 0.585 0.165 0.978 0.065 0.705 0.925 0.435 0.597 0.991 0.170 0.080 0.982 0.001 0.117 0.013 0.756 0.756 M315 0.004 0.000 0.025 0.003 0.180 0.002 0.051 0.120 0.032 0.026 0.219 0.003 0.003 0.187 0.000 0.003 0.000