2008 National Cotton Fusarium Wilt Report November 2008 Agronomy And SoilS depArtment SerieS no. 296 AlAbAmA AgriculturAl experiment StAtion richArd guthrie, director Auburn univerSity Auburn, Al 36849 This reporT is a joinT conTribuTion beTween usDa-ars, crop science research LaboraTory, Mississippi sTaTe universiTy, Mississippi, anD The aLabaMa agricuLTuraL experiMenT sTaTion, auburn universiTy, aLabaMa Information contained herein is available to all persons regardless of race, color, sex, or national origin 2008 NATIONAL COTTON FUSARIUM WILT REPORT 3 2008 NATIONAL COTTON FUSARIUM WILT REPORT Kathryn M. Glass1, William S. Gazaway2, Katheryn Lawrence4, and Edzard van Santen 3 Advisor III, Natl Res. Programs and Professor, respectively, Dept. of Agronomy and Soils, Auburn University, AL 36849 2,4 Professor Emeritus and Extension Pathologist/Nematologist and Associate Professor, respectively, Dept. of Entomology and Plant Pathology, Auburn University, 36849 1,3 Cotton cultivars and elite breeding lines submitted by 24 cooperators were evaluated for Fusarium wilt resistance under field conditions at the E. V. Smith Research Center, Plant Breeding Unit, Tallassee, Alabama. These entries were grown on an Wickham loamy fine sand highly infested with the Fusarium wilt fungus (Fusarium oxysporum) Schlect. f. vasinfectum [Atk.] (Snyd. & Hans.) and southern root-knot nematodes (Meloidogyne incognita.). In 2008, a soil analysis for nematodes revealed that southern root-knot (Meloidogyne incognita) was the predominant nematode species in the test plots. The North Fusarium wilt field plot contains a population of M. incognita that ranges from 155 to 1546 J2 per 150 cc of soil with an mean of 711 J2. The populations in the South Fusarium wilt field are lower with a range from 77 to 1004 J2 per 150 cc of soil and a mean population of 378. Other nematode genera present are stubby root (Trichodorus sp.) and stunt (Tylenchorhynchus sp.). Root-knot nematodes, however, appear to be causing the major damage to cotton in the Fusarium Wilt Test as indicated by the high galling indices found on the roots of all cotton lines. The root-knot nematode population throughout the entire test area, i.e., even the areas with the lowest root-knot nematode populations, is more than sufficient to cause a high incidence of Fusarium wilt. Entries were planted in single 20-foot rows on 36-inch centers, separated by 5-foot alleys. Four replications of the test entries and checks were evaluated in a randomized complete block design with a split plot restriction on randomization. The set of eight test cultivars submitted by a cooperator was always evaluated as a group together with two control plots within each replicate. Both susceptible (Rowden) and resistant (M-315) cultivars were included as check subplots in the two center rows of each main plot (Fig. 1). The test was planted on May 19. Initial plant counts were made on June 12. Wilted plants were counted and removed on June 25, July 10, July 24, August 8, and August 22. The remaining live plants were counted and recorded on September 26. Total percent wilted plants were then determined and mean wilting for a given entry calculated. Wilt development was quite uniform in all blocks with rep averages ranging from 11 to 26% averaged over all entries. The average % wilted plants for the susceptible check Rowden was 61%, with a range from 6 to 100% on an individual plot basis (Fig. 1). The resistant check M-315 had an average of 4% wilted plants, with a range of 0 to 28%. Critical evaluations of breeding lines should be made relative to the Rowden check listed at the bottom of each group. 4 ALABAMA AGRICULTURAL EXPERIMENT STATION Fig. 1. Field plot layout for the North Wilt Field and % wilt for control plot of Rowden (suceptible) and M-315 (resistant). Distances (ft) from the SE corner of the trial are given in the left hand column and the bottom row. EW 575 550 525 500 475 450 425 400 375 350 325 0 M-315 4205 20 Rowden 4165 44 Rowden 4125 7 M-315 4085 62 Rowden 4045 1 M-315 4005 2 M-315 3205 1 M-315 3165 2 M-315 3125 43 Rowden 3085 0 M-315 3045 87 Rowden 3005 97 Rowden 2205 99 Rowden 2165 4 M-315 2125 13 M-315 2085 95 Rowden 2045 6 M-315 2005 98 Rowden 1205 96 Rowden 1165 3 M-315 1125 0 M-315 1085 0 M-315 1045 0 M-315 1005 24 12 Rowden 4206 6 M-315 4166 0 M-315 4126 71 Rowden 4086 0 M-315 4046 39 Rowden 4006 49 Rowden 3206 23 Rowden 3166 24 Rowden 3126 0 M-315 3086 71 Rowden 3046 28 M-315 3006 14 M-315 2206 6 M-315 2166 96 Rowden 2126 99 Rowden 2086 9 M-315 2046 100 Rowden 2006 4 M-315 1206 9 M-315 1166 94 Rowden 1126 85 Rowden 1086 67 Rowden 1046 75 Rowden 1006 4 M-315 4215 2 M-315 4175 71 Rowden 4135 6 M-315 4095 2 M-315 4055 23 Rowden 4015 22 Rowden 3215 0 M-315 3175 5 M-315 3135 0 M-315 3095 0 M-315 3055 65 Rowden 3015 84 Rowden 2215 3 M-315 2175 9 M-315 2135 14 M-315 2095 69 Rowden 2055 80 Rowden 2015 88 Rowden 1215 6 M-315 1175 1 M-315 1135 6 M-315 1095 1 M-315 1055 1 M-315 1015 54 26 Rowden 4216 54 Rowden 4176 3 M-315 4136 60 Rowden 4096 61 Rowden 4056 1 M-315 4016 2 M-315 3216 11 Rowden 3176 30 Rowden 3136 27 Rowden 3096 33 Rowden 3056 4 M-315 3016 3 M-315 2216 92 Rowden 2176 88 Rowden 2136 80 Rowden 2096 4 M-315 2056 1 M-315 2016 1 M-315 1216 58 Rowden 1176 61 Rowden 1136 34 Rowden 1096 44 Rowden 1056 48 Rowden 1016 51 Rowden 4225 6 M-315 4185 2 M-315 4145 8 M-315 4105 23 Rowden 4065 8 Rowden 4025 6 Rowden 3225 6 M-315 3185 15 Rowden 3145 10 M-315 3105 9 Rowden 3065 14 Rowden 3025 4 M-315 2225 7 M-315 2185 2 M-315 2145 85 Rowden 2105 5 M-315 2065 4 M-315 2025 3 M-315 1225 4 M-315 1185 57 Rowden 1145 36 Rowden 1105 43 Rowden 1065 71 Rowden 1025 0 M-315 4226 36 Rowden 4186 68 Rowden 4146 78 Rowden 4106 2 M-315 4066 1 M-315 4026 6 M-315 3226 42 Rowden 3186 4 M-315 3146 26 Rowden 3106 0 M-315 3066 4 M-315 3026 52 Rowden 2226 74 Rowden 2186 65 Rowden 2146 3 M-315 2106 65 Rowden 2066 75 Rowden 2026 62 Rowden 1226 38 Rowden 1186 5 M-315 1146 3 M-315 1106 4 M-315 1066 5 M-315 1026 5 M-315 4235 63 Rowden 4195 1 M-315 4155 1 M-315 4115 54 Rowden 4075 0 M-315 4035 3 M-315 3235 44 Rowden 3195 41 Rowden 3155 79 Rowden 3115 2 M-315 3075 82 Rowden 3035 91 Rowden 2235 8 M-315 2195 95 Rowden 2155 98 Rowden 2115 94 Rowden 2075 95 Rowden 2035 4 M-315 1235 6 M-315 1195 2 M-315 1155 6 M-315 1115 0 M-315 1075 6 M-315 1035 114 11 Rowden 4236 4 M-315 4196 76 Rowden 4156 59 Rowden 4116 1 M-315 4076 70 Rowden 4036 49 Rowden 3236 1 M-315 3196 2 M-315 3156 3 M-315 3116 73 Rowden 3076 8 M-315 3036 3 M-315 2236 96 Rowden 2196 8 M-315 2156 15 M-315 2116 7 M-315 2076 3 M-315 2036 81 Rowden 1236 99 Rowden 1196 94 Rowden 1156 99 Rowden 1116 99 Rowden 1076 96 Rowden 1036 8 rows 8 rows 8 rows 8 rows 300 275 250 225 200 175 150 125 100 75 50 25 0 SN N 84 8 rows 2008 NATIONAL COTTON FUSARIUM WILT REPORT 5 Table 1. Percent wilted plants for entries and check in each replicate, least squares estimate of the average, P-value based on Dunnett’s versus the resistant check M-315, and initial average number of plants per plot. Entry Cultivar/Line Rep1 Percent wilted plants Rep 2 Rep 3 Rep 4 Avg. no. Avg. P-value of plants 79521 5 0.007 4 0.012 5 0.009 4 0.010 16 <0.0001 2 0.127 15 <0.0001 4 0.025 57 <0.0001 1 27893 5 0.254 59 <0.0001 36 <0.0001 7 0.087 12 0.001 38 <0.0001 18 <0.0001 7 0.088 85 <0.0001 4 88 77 81 86 86 83 82 87 87 83 81 84 88 85 83 88 80 85 78 81 76 74 75 70 68 73 78 70 79 77 Gary L. Rea, Delta and Pine Land Co., 247 US HWY 380 W, Haskell, TX 101 GLR-1 0 10 5 7 102 GLR-2 4 5 6 7 103 GLR-3 10 3 4 4 104 GLR-4 5 13 3 0 105 GLR-5 12 41 6 10 106 GLR-6 0 3 2 5 107 GLR-7 5 35 26 1 108 GLR-8 2 2 10 2 Rowden 36 95 43 51 M-315 3 3 0 0 Laura Barham, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 201 LB-1 7 10 1 5 202 LB-2 35 78 52 67 203 LB-3 23 64 40 22 204 LB-4 2 8 12 6 205 LB-5 31 5 7 9 206 LB-6 66 66 18 2 207 LB-7 32 35 9 1 208 LB-8 7 9 5 8 Rowden 99 80 79 78 M-315 6 1 3 8 O. Lloyd May, Delta and Pine Land Co., 381 William Gibbs Rd, Tifton, GA 31794 301 LM-1 87 14 30 14 34 <0.0001 302 LM-2 75 42 26 1 33 <0.0001 303 LM-3 31 9 2 9 10 <0.0001 304 LM-4 15 4 13 3 7 0.013 305 LM-5 92 10 46 12 39 <0.0001 306 LM-6 1 6 3 3 2 0.702 307 LM-7 11 6 7 11 6 0.014 308 LM-8 3 6 10 6 5 0.095 Rowden 96 85 73 61 82 <0.0001 M-315 9 3 2 2 3 † The number listed in the average column is the estimate of the average wilt percentage based on a generalized linear mixed model with the binomial distribution for fixed effects. This estimate will generally be close, but may or may not be identical, to the arithmetic average obtained by averaging the numbers in the columns representing the 4 reps. 6 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 1. continued Entry Cultivar/Line Rep 1 Percent wilted plants Rep 2 Rep 3 Rep 4 Avg. no. Avg. P-value of plants 69 84 73 76 71 77 77 73 83 80 80 77 74 76 77 67 69 80 78 79 79 58 56 68 63 63 67 57 87 77 Darren Jones, Monsanto Company, 4600 Old Leland Rd, Leland, MS 38756 401 DJ-1 12 16 15 0 8 0.048 402 DJ-2 17 15 15 1 9 0.016 403 DJ-3 9 22 39 0 13 <0.0001 404 DJ-4 0 21 4 5 6 0.323 405 DJ-5 11 51 11 4 15 <0.0001 406 DJ-6 12 9 16 1 7 0.102 407 DJ-7 24 15 4 5 10 0.010 Kathryn M Glass, Auburn University, 201 Funchess Hall, Auburn, AL 36849 408 ST 4498 B2RF 3 11 10 3 5 0.594 Rowden 34 96 82 11 55 <0.0001 M-315 6 8 8 5 5 Laval Verhalen, Oklahoma State University, 368 Agricultural Hall, Stillwater, OK 501 OKLA-1 21 9 3 0 5 0.108 502 OKLA-2 20 18 13 5 9 0.001 503 OKLA-3 11 11 3 2 4 0.286 504 OKLA-4 51 23 8 11 16 <0.0001 505 OKLA-5 67 75 10 6 34 <0.0001 506 OKLA-6 13 13 1 2 4 0.236 507 OKLA-7 64 26 6 0 16 <0.0001 508 OKLA-8 11 13 0 2 4 0.327 Rowden 94 95 26 12 59 <0.0001 M-315 3 8 10 0 3 Charlie Cook, All-Tex Seeds, Inc., 356 Hosek Road, Victoria, TX 77905-5636 601 CC-1 3 7 5 4 3 0.254 602 CC-2 20 1 2 9 7 0.008 603 CC-3 3 5 27 7 9 0.001 604 CC-4 3 17 0 14 7 0.006 605 CC-5 2 5 29 3 7 0.005 606 CC-6 0 30 23 2 11 <0.0001 607 CC-7 2 8 3 1 3 0.509 608 CC-8 11 93 73 6 43 <0.0001 Rowden 44 99 71 62 71 <0.0001 M-315 1 13 0 0 3 2008 NATIONAL COTTON FUSARIUM WILT REPORT 7 Table 1. continued Entry Cultivar/Line Rep 1 Percent wilted plants Rep 2 Rep 3 Rep 4 Avg. no. Avg. P-value of plants 79041 2 0.932 5 0.355 16 <0.0001 8 0.034 3 0.739 5 0.257 7 0.058 33 <0.0001 63 <0.0001 4 79 86 78 83 91 84 77 73 81 73 71 76 74 58 73 74 80 76 80 77 87 88 84 80 85 86 83 88 82 72 Richard Sheetz, Delta and Pine Land Co., RR 2, Box 60, Hale Center, TX 701 RHS-1 2 0 3 3 702 RHS-2 4 12 1 5 703 RHS-3 27 38 1 4 704 RHS-4 4 18 12 1 705 RHS-5 5 4 3 3 706 RHS-6 1 17 3 3 707 RHS-7 13 11 4 6 708 RHS-8 17 41 30 44 Rowden 62 88 27 71 M-315 3 9 0 7 Curtis Williams, Delta and Pine Land Co., 381 William Gibbs Rd, Tifton, GA 31794 801 CW-1 36 41 1 0 15 <0.0001 802 CW-2 4 10 4 0 3 0.790 803 CW-3 100 27 34 53 52 <0.0001 804 CW-4 76 75 7 19 42 <0.0001 805 CW-5 7 55 8 6 15 <0.0001 806 CW-6 35 41 5 14 20 <0.0001 807 CW-7 9 14 1 5 5 0.309 808 CW-8 36 10 2 13 11 0.001 Rowden 98 97 49 68 83 <0.0001 M-315 4 14 3 2 4 Steve Calhoun, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 901 SC-1 12 30 13 22 902 SC-2 25 24 7 52 903 SC-3 10 100 19 16 904 SC-4 2 8 5 8 905 SC-5 42 94 22 19 906 SC-6 11 23 0 5 907 SC-7 11 13 2 26 908 SC-8 1 14 3 2 Rowden 43 96 23 23 M-315 4 4 1 2 27893 17 <0.0001 24 <0.0001 33 <0.0001 5 0.035 43 <0.0001 8 0.001 10 <0.0001 4 0.092 46 <0.0001 2 8 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 1. continued Entry Cultivar/Line Rep 1 Percent wilted plants Rep 2 Rep 3 Rep 4 Avg. no. Avg. P-value of plants 66 58 59 53 60 54 75 75 84 72 75 71 67 66 79 73 68 78 84 73 80 92 91 83 83 77 69 59 78 78 Jack E. Jones, Jajo Genetics, 246 Maxine Dr., Baton Rouge, LA 70808-6831 1001 JJ-1 89 11 12 5 24 <0.0001 1002 JJ-2 15 51 2 3 12 <0.0001 1003 JJ-3 50 20 43 7 25 <0.0001 1004 JJ-4 44 33 0 7 17 <0.0001 1005 JJ-5 25 16 6 0 9 <0.0001 1006 JJ-6 16 33 28 11 19 <0.0001 1007 JJ-7 11 8 1 5 5 0.022 1008 JJ-8 4 14 3 0 4 0.063 Rowden 85 92 9 60 64 <0.0001 M-315 0 3 0 6 2 Dawn Fraser, Delta and Pine Land Co., P.O. Box 1529, Hartsville, SC 29550 1101 DF-1 39 65 23 4 28 <0.0001 1102 DF-2 19 12 0 7 7 0.283 1103 DF-3 2 5 2 2 2 0.993 1104 DF-4 20 30 2 4 10 0.031 1105 DF-5 4 4 1 0 2 0.997 1106 DF-6 69 16 4 6 19 <0.0001 1107 DF-7 3 17 3 4 5 0.700 1108 DF-8 11 9 5 0 4 0.813 Rowden 96 80 42 20 61 <0.0001 M-315 6 14 6 6 6 Frank Bordelon, PhytoGen Cottonseed, P.O. Box 27, Leland, MS 38756 1201 PHY-FB1 4 11 16 3 7 0.033 1202 PHY-FB2 3 4 1 9 4 0.516 1203 PHY-FB3 4 9 4 5 5 0.232 1204 PHY-FB4 6 15 3 3 6 0.113 1205 PHY-FB5 2 4 8 2 4 0.545 1206 PHY-FB6 25 41 4 37 26 <0.0001 1207 PHY-FB7 9 6 4 6 6 0.161 1208 PHY-FB8 7 2 2 0 2 0.841 Rowden 57 65 6 8 34 <0.0001 M-315 5 5 6 1 4 2008 NATIONAL COTTON FUSARIUM WILT REPORT 9 Table 1. continued Entry Cultivar/Line Rep 1 Percent wilted plants Rep 2 Rep 3 Rep 4 Avg. no. Avg. P-value of plants 71 72 61 71 59 63 57 61 79 78 87 84 88 82 81 86 87 90 81 80 83 85 89 88 86 82 83 77 79 77 Peggy Thaxton, Mississippi State University, P.O. Box 1301 DREC-1 93 69 1302 DREC-2 38 7 1303 DREC-3 9 19 1304 DREC-4 24 17 1305 DREC-5 18 3 1306 DREC-6 0 2 1307 DREC-7 0 13 1308 DREC-8 13 10 Rowden 99 94 M-315 6 7 197, Stoneville, MS 38776 17 33 54 <0.0001 3 15 12 <0.0001 3 30 12 <0.0001 3 3 8 0.001 3 11 7 0.014 4 3 1 0.918 0 0 3 0.604 0 1 4 0.164 44 71 81 <0.0001 1 3 3 David Miller, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 27893 1401 DM-1 8 21 4 8 9 0.001 1402 DM-2 29 8 5 2 8 0.002 1403 DM-3 8 5 3 2 4 0.234 1404 DM-4 8 4 1 9 5 0.093 1405 DM-5 4 6 3 9 5 0.131 1406 DM-6 5 2 2 1 2 0.776 1407 DM-7 9 23 4 2 8 0.005 1408 DM-8 8 1 1 4 3 0.501 Rowden 81 75 15 26 49 <0.0001 M-315 4 4 4 4 3 Michael Swindle, Bayer Crop Science, 117 Kennedy Flat Rd., Leland, MS 38756 1501 BCSI-MS-1 5 14 25 31 18 <0.0001 1502 BCSI-MS-2 2 4 3 5 3 0.113 1503 BCSI-MS-3 18 75 16 35 35 <0.0001 1504 BCSI-MS-4 1 8 6 1 4 0.062 1505 BCSI-MS-5 2 6 1 2 3 0.159 1506 BCSI-MS-6 2 1 0 0 1 0.750 1507 BCSI-MS-7 26 5 12 7 12 <0.0001 1508 BCSI-MS-8 5 0 4 1 2 0.209 Rowden 48 84 33 54 54 <0.0001 M-315 1 3 0 2 2 10 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 1. continued Entry Cultivar/Line Rep 1 Percent wilted plants Rep 2 Rep 3 Rep 4 Avg. no. Avg. P-value of plants <0.0001 <0.0001 0.001 0.001 <0.0001 0.001 0.098 0.018 <0.0001 81 78 74 80 55 85 84 83 79 83 78 76 72 67 85 82 84 54 79 85 90 88 85 78 85 86 88 88 83 79 Joe Johnson, Bayer Crop Science, 117 Kennedy Flat Rd., Leland, MS 38756 1601 BCSI-JJ-1 83 58 16 15 40 1602 BCSI-JJ-2 68 80 27 18 48 1603 BCSI-JJ-3 17 6 4 5 5 1604 BCSI-JJ-4 5 16 4 5 5 1605 BCSI-JJ-5 7 20 7 5 6 1606 BCSI-JJ-6 20 6 1 2 5 1607 BCSI-JJ-7 1 7 1 1 2 1608 BCSI-JJ-8 1 12 5 0 3 Rowden 99 69 11 23 49 M-315 0 4 0 1 1 Mustafa McPherson, PhytoGen Cottonseed, P.O. Box 27, Leland, MS 38756 1701 PHY- MM1 14 5 4 2 5 0.007 1702 PHY- MM2 5 6 0 7 4 0.035 1703 PHY- MM3 6 1 3 3 2 0.182 1704 PHY- MM4 8 4 2 3 3 0.070 1705 PHY- MM5 21 8 0 6 7 0.001 1706 PHY- MM6 12 41 7 8 14 <0.0001 1707 PHY- MM7 7 2 0 1 2 0.269 1708 PHY- MM8 47 77 9 3 28 <0.0001 Rowden 94 65 49 39 64 <0.0001 M-315 2 2 2 1 2 Mike 1801 1802 1803 1804 1805 1806 1808 1808 MR-1 MR-2 MR-3 MR-4 MR-5 MR-6 MR-8 MR-8 Rowden M-315 24 34 50 19 11 89 5 5 67 0 5 5 9 4 6 55 3 3 74 7 3 12 6 1 7 17 5 5 14 4 4 7 2 8 1 35 2 2 70 0 7 11 13 6 5 49 3 3 57 2 0.001 <0.0001 <0.0001 0.005 0.022 <0.0001 0.265 0.265 <0.0001 2008 NATIONAL COTTON FUSARIUM WILT REPORT 11 Table 1. continued Entry Cultivar/Line Rep 1 Percent wilted plants Rep 2 Rep 3 Rep 4 Avg. no. Avg. P-value of plants 4 15 18 8 14 4 14 5 59 3 6 15 10 4 4 3 0.325 <0.0001 <0.0001 0.005 <0.0001 0.161 <0.0001 0.070 <0.0001 79 68 65 69 69 66 76 65 86 81 70 70 75 77 70 76 Albert Santos, Delta and Pine Land Co., P.O. Box 157, Scott, MS 38772 1901 SM-1 3 8 0 5 1902 SM-2 21 29 14 3 1903 SM-3 3 15 11 40 1904 SM-4 3 21 8 4 1905 SM-5 26 16 4 11 1906 SM-6 1 6 5 7 1907 SM-7 11 23 14 11 1908 SM-8 3 17 0 5 Rowden 71 91 30 44 M-315 5 3 5 0 Rex Green, Delta and Pine Land Co., P.O. Box 157, Scott, MS 38772 2001 LMS-1 11 11 3 3 2002 LMS-2 23 24 6 15 2003 LMS-3 13 16 8 7 2004 LMS-4 6 4 4 6 2005 LMS-5 1 0 4 11 2006 LMS-6 2 3 4 7 2007 2008 Rowden 58 98 22 36 M-315 6 15 2 6 Fred Bourland, University of Arkansas, P.O. Box 48, Keiser, AR 72351 2101 FB-1 20 6 15 2 2102 FB-2 5 25 6 3 2103 FB-3 21 33 14 5 2104 FB-4 10 16 3 5 2105 FB-5 6 42 5 0 2106 FB-6 13 1 0 6 2107 FB-7 9 22 8 4 2108 FB-8 15 12 3 0 Rowden 88 99 24 59 M-315 1 6 2 1 0.578 0.001 0.060 0.873 0.918 0.960 53 <0.0001 6 9 7 16 6 9 4 9 6 69 2 <0.0001 0.001 <0.0001 0.004 <0.0001 0.051 0.001 0.010 <0.0001 84 78 66 69 62 68 58 74 60 69 75 79 12 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 1. continued Entry Cultivar/Line Rep 1 Percent wilted plants Rep 2 Rep 3 Rep 4 Avg. no. Avg. P-value of plants 0.201 <0.0001 <0.0001 <0.0001 0.388 0.531 <0.0001 <0.0001 <0.0001 62 71 47 61 73 60 68 68 77 71 83 82 85 86 64 59 61 55 79 80 81 88 80 81 77 74 79 70 75 72 Shirley Howard, Delta and Pine Land Co., P.O. Box 157, Scott, MS 38772 2201 SH-1 4 25 17 3 9 2202 SH-2 21 80 3 7 21 2203 SH-3 26 68 18 13 27 2204 SH-4 13 75 86 33 50 2205 SH-5 26 7 8 4 8 2206 SH-6 4 28 7 2 7 2207 SH-7 17 55 82 9 38 2208 SH-8 4 6 63 10 18 Rowden 61 100 87 54 79 M-315 1 6 28 1 8 Ammeta Mobley, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 2301 AM-1 4 4 5 3 2302 AM-2 1 3 4 13 2303 AM-3 8 1 1 1 2304 AM-4 2 2 0 5 Cody Poage, All-Tex Seeds, Inc., P.O. Box 1057, Levelland, TX 79336 2305 CP-1 10 5 1 19 2306 CP-2 2 6 3 4 2307 CP-3 6 0 12 6 2308 CP-4 15 4 6 8 Rowden 38 52 41 76 M-315 4 4 2 1 27893 4 0.228 5 0.086 2 0.561 2 0.632 8 0.003 4 0.278 6 0.037 8 0.004 51 <0.0001 3 <0.0001 0.005 <0.0001 0.267 <0.0001 <0.0001 0.001 <0.0001 <0.0001 Jeff Klingenberg, Bayer Crop Science, 1602 Paradise Dr., Sellers, SC 25992 2401 BCSI-JK-1 38 34 29 2 24 2402 BCSI-JK-2 12 10 10 7 8 2403 BCSI-JK-3 26 61 4 7 21 2404 BCSI-JK-4 3 10 6 4 4 2405 BCSI-JK-5 1 47 11 9 13 2406 BCSI-JK-6 35 33 6 1 15 2407 BCSI-JK-7 10 33 5 4 10 2408 BCSI-JK-8 46 40 12 2 22 Rowden 75 95 65 63 77 M-315 0 9 4 4 3