1999 NATIONAL FUSARIUM WILT COTTON REPORT 1 2 ALABAMA AGRICULTURAL EXPERIMENT STATION THIS REPORT IS A JOINT CONTRIBUTION BETWEEN USDA-ARS, C ROP SCIENCE RESEARCH LABORATORY , MISSISSIPPI STATE UNIVERSITY, MISSISSIPPI , AND THE A LABAMA A GRICULTURAL E XPERIMENT S TATION , A UBURN U NIVERSITY , ALABAMA . Information contained herein is available to all persons regardless of race, color, sex, or national origin. 1999 NATIONAL FUSARIUM WILT COTTON REPORT 3 1999 National Fusarium Wilt Cotton Report K. M. Glass and W. S. Gazaway1 Cotton cultivars and elite breeding lines submitted by 28 cooperators were evaluated for fusarium wilt resistance under field conditions at the E. V. Smith Research Center, Plant Breeding Unit, Tallassee, Alabama. These entries were grown on an Independence loamy fine sand highly infested with both the fusarium wilt fungus (Fusarium oxysporum) Schlect. f. vasinfectum [Atk.] (Snyd. & Hans.) and root-knot nematodes (Meloidogyne incognita). Plots were 40-inch-wide rows, 20 feet in length, separated by 5-foot alleys. Four replications of the test entries and checks, arranged in a block design, were evaluated. Both susceptible (Rowden) and resistant (M-315) cultivars were included as checks. Rowden was planted in row five and every tenth row thereafter (15, 25,...,265), and M-315 in row 10 and every tenth row thereafter (20, 30,...,270) throughout the test. Plots were planted May 18. Initial plant counts were made on June 23. Wilted plants were counted and removed on July 8, July 29, and August 19. The remaining live plants were counted and recorded on September 10. Percent of wilted plants was then determined and mean wilting for a given entry calculated. Average wilting of the susceptible Rowden was 31, 33, 21, and 21% for the four replications (27% average). Corresponding wilt percentages for the resistant check, M-315, were 0.7, 1, 2, and 1% (1% average). Critical evaluation of a given entry should be made relative to the checks closest to the entry within each replication. Evaluation of breeding progress or evaluation of entries over years should be made only between the relative value of this entry and that of the closest susceptible check rows for each year. In 1994, a soil analysis for nematodes revealed that southern root-knot (Meloidogyne incognita) and lance (Hoplolaimus galeatus) are two predominant nematode species in the test plots. High populations of both species are found throughout the test area. Other nematode genera present are stubby root (Trichodorus sp.) and stunt (Tylenchorhynchus sp.). Root-knot nematodes, however, appear to be causing the major damage to cotton in the Fusarium Wilt Test as indicated by the high galling indices found on the roots of all cotton lines. Fusarum wilt was light on all varieties including the susceptible cultivar Rowden. Early dry conditions caused plants to drop leaves early and made rating wilt difficult. Entries submitted by Kathryn Glass are commonly grown cultivars or advanced commercial materials and are listed by name. Entries submitted by other cooperators are listed by their coded numbers. Additional information regarding the genetic background of a specific coded entry should be obtained from the named cooperator. Glass is an Agricultural Program Associate, in the Department of Agronomy and Soils, and Gazaway is Professor and Extension Plant Pathologist/Nematologist, in the Department of Entomology and Plant Pathology, both at Auburn University. 1 4 ALABAMA AGRICULTURAL EXPERIMENT STATION 1999 FUSARIUM WILT TEST, E. V. S MITH RESEARCH CENTER, TALLASSEE , A LABAMA Test entry designation 1 001 002 003 004 005 006 007 008 009 010 2 011 012 013 014 015 016 017 018 019 020 3 021 022 023 024 025 026 027 028 029 030 4 031 032 033 034 035 036 037 038 039 040 ——————Percent wilt by replication—————— 1 2 3 4 Mean Curtis Williams, Paymaster Cottonseed, 2476 Hwy. 130 East, Stuttgart, AR 72160 CW-1 ................................................. 1 CW-2 ................................................. 0 CW-3 ............................................... 10 CW-4 ................................................. 0 ROWDEN ....................................... 15 CW-5 ................................................. 1 CW-6 ................................................. 0 CW-7 ................................................. 0 CW-8 ................................................. 3 M-315 ................................................ 0 0 0 11 10 24 0 4 2 2 7 0 0 6 1 12 5 9 2 2 8 0 6 2 3 21 0 0 0 0 7 0 2 7 4 18 2 3 1 2 6 Roy Creech, USDA-ARS, Mississipi State, MS 39762 1 ......................................................... 0 2 ......................................................... 2 3 ......................................................... 1 4 ......................................................... 3 ROWDEN ....................................... 49 5 ......................................................... 0 6 ......................................................... 1 7 ......................................................... 0 8 ......................................................... 0 M-315 ................................................ 0 4 3 2 0 39 6 2 3 7 0 8 1 1 10 7 0 2 0 1 0 0 0 4 3 6 2 0 9 3 0 3 2 2 4 25 2 1 3 3 0 Charles Cook, United Agri Products, P.O. Box 1149, Santa Rosa, TX 78593 CGC-1 ................................................ 2 CGC-2 ................................................ 8 CGC-3 ................................................ 9 CGC-4 ................................................ 1 ROWDEN ....................................... 31 CGC-5 ................................................ 4 CGC-6 .............................................. 11 CGC-7 ................................................ 3 CGC-8 ................................................ 0 M-315 ................................................ 0 0 30 25 28 37 18 0 4 0 0 2 5 13 0 16 2 0 0 4 0 13 14 14 15 33 0 2 0 3 0 4 15 15 11 29 6 3 3 1 0 Laval M. Verhalen, Oklahoma State University, 368 Agricultural Hall, Stillwater, OK 74078-6028 OKLA-1 ............................................. 1 OKLA-2 ............................................. 9 OKLA-3 ............................................. 1 OKLA-4 ............................................. 0 ROWDEN ....................................... 44 OKLA-5 ............................................. 1 OKLA-6 ............................................. 1 OKLA-7 ............................................. 0 OKLA-8 ............................................. 2 M-315 ................................................ 0 4 0 0 7 16 3 7 0 3 0 0 3 3 1 2 0 0 0 3 2 6 9 2 11 11 1 5 3 4 0 3 5 1 5 18 1 3 1 3 1 continued 1999 NATIONAL FUSARIUM WILT COTTON REPORT 5 1999 FUSARIUM WILT TEST, E. V. SMITH RESEARCH CENTER, TALLASSEE , A LABAMA Test entry designation 5 041 042 043 044 045 046 047 048 049 050 6 051 052 053 054 055 056 057 058 059 060 7 061 062 063 064 065 066 067 068 069 070 8 071 072 073 074 075 076 077 078 079 080 ——————Percent wilt by replication—————— 1 2 3 4 Mean Terry Weesner, Sure Grow Research, P.O. Box 589, Maricopa, AZ 85239 AZ-1 ................................................... 0 AZ-2 ................................................... 0 AZ-3 ................................................... 0 AZ-4 ................................................... 0 ROWDEN ....................................... 20 AZ-5 ................................................... 6 AZ-6 ................................................... 0 AZ-7 ................................................... 0 AZ-8 ................................................... 2 M-315 ................................................ 0 0 0 5 0 35 4 4 4 10 2 2 2 4 3 34 0 2 0 5 0 0 3 0 0 12 0 0 3 3 0 0 1 2 1 25 2 1 2 5 1 Peggy Thaxton, Dept. of Soil & Crop Sci., Texas A&M Univ., College Station, TX 77843-2474 PMT-1 ................................................ 0 PMT-2 ................................................ 3 PMT-3 ................................................ 3 PMT-4 ................................................ 0 ROWDEN ....................................... 21 PMT-5 ................................................ 3 PMT-6 ................................................ 1 PMT-7 .............................................. 39 PMT-8 .............................................. 23 M 315 ................................................ 0 41 17 13 6 25 5 10 0 6 0 44 11 11 0 34 6 1 6 0 5 10 2 3 2 23 13 4 0 2 0 24 8 8 2 26 7 4 11 8 1 Jim Mitchell, Paymaster Cottonseed, 2476 Hwy. 130 East , Stuttgart, AR 72160 JM-1 ................................................. 6 JM-2 ................................................. 24 JM-3 ................................................... 7 JM-4 ................................................. 17 ROWDEN ....................................... 30 JM-5 ................................................... 2 JM-6 ................................................... 0 JM-7 ................................................... 1 JM-8 ................................................... 3 M-315 ................................................ 2 0 1 0 14 17 0 3 4 0 0 2 2 9 6 20 0 15 4 5 0 0 0 0 0 12 1 2 7 2 0 2 7 4 9 20 1 5 4 3 1 Don Keim, Delta and Pine Land Co., 100 Main Street, Scott, MS 38772 1 ......................................................... 0 2 ......................................................... 0 3 ......................................................... 0 4 ......................................................... 0 ROWDEN ....................................... 33 5 ......................................................... 4 6 ......................................................... 3 7 ......................................................... 3 8 ......................................................... 0 M-315 ................................................ 0 2 2 1 4 66 19 4 1 0 0 3 2 0 11 35 11 2 15 6 0 6 2 0 4 24 15 3 6 0 0 3 1 0 5 40 12 3 6 1 0 continued 6 ALABAMA AGRICULTURAL EXPERIMENT STATION 1999 F USARIUM WILT TEST, E. V. SMITH RESEARCH CENTER, TALLASSEE , ALABAMA Test entry designation 9 081 082 083 084 085 086 087 088 089 090 10 091 092 093 094 095 096 097 098 099 100 11 101 102 103 104 105 106 107 108 109 110 12 111 112 113 114 115 116 117 118 119 120 ——————Percent wilt by replication—————— 1 2 3 4 Mean Randall McPherson, Phytogen Seed Co., P.O. Box 27, Leland, MS 38756 PX-1 ................................................... 1 PX-2 ................................................... 2 PX-3 ................................................... 2 PX-4 ................................................... 1 ROWDEN ....................................... 44 PX-5 ................................................... 2 PX-6 ................................................... 0 PX-7 ................................................... 3 PX-8 ................................................... 0 M-315 ................................................ 0 1 0 0 2 33 0 1 0 2 0 3 0 0 5 6 0 4 7 3 3 0 5 1 8 22 3 3 5 0 0 2 2 1 4 26 1 2 4 1 1 John Green, Seed Source Inc., P.O. Box 28, Stoneville, MS 38776 SS-1 ................................................... 0 SS-2 ................................................... 4 SS-3 ................................................... 7 SS-4 ................................................... 2 ROWDEN ....................................... 35 SS-5 ................................................... 0 SS-6 ................................................... 0 SS-7 ................................................... 4 SS-8 ................................................... 3 M-315 ................................................ 0 0 3 0 7 20 0 10 2 0 3 0 0 2 6 8 12 2 0 4 0 0 7 0 16 19 5 2 6 0 0 0 3 2 8 21 4 4 3 2 1 Ed Lubber, Phytogen Seed Co., P.O. Box 27, Leland, MS 38756 PX-9 ................................................... 2 PX-10 ................................................. 2 PX-11 ................................................. 0 PX-12 ................................................. 2 ROWDEN ....................................... 10 PX-13 ................................................. 2 PX-14 ................................................. 0 PX-15 ................................................. 2 PX-16 ................................................. 2 M-315 ................................................ 0 4 7 3 17 48 5 2 4 7 0 40 4 11 3 26 8 5 5 2 0 0 0 32 3 20 6 2 0 0 0 11 3 11 6 26 5 2 3 3 0 Cindy Green, Delta and Pine Land Co., P.O. Box 1529, Hartsville, SC 29551 1 ......................................................... 0 2 ......................................................... 1 3 ......................................................... 3 4 ......................................................... 2 ROWDEN ....................................... 19 5 ....................................................... 22 6 ....................................................... 12 7 ....................................................... 37 8 ....................................................... 34 M-315 ................................................ 2 3 0 5 0 39 14 1 1 0 1 4 0 2 36 40 9 0 13 17 3 4 21 4 4 8 12 0 4 0 0 3 6 3 10 27 14 3 14 13 2 continued 1999 NATIONAL FUSARIUM WILT COTTON REPORT 7 1999 FUSARIUM WILT T EST, E. V. S MITH RESEARCH CENTER, T ALLASSEE , ALABAMA Test entry designation 13 121 122 123 124 125 126 127 128 129 130 14 131 132 133 134 135 136 137 138 139 140 15 141 142 143 144 145 146 147 148 149 150 16 151 152 153 154 155 156 157 158 159 160 ——————Percent wilt by replication—————— 1 2 3 4 Mean C. Wayne Smith, Dept. of Soil & Crop Sci., Texas A & M Univ., College Station, TX 77843-2474 1 ....................................................... 88 2 ......................................................... 8 3 ....................................................... 33 4 ....................................................... 42 ROWDEN ....................................... 44 5 ......................................................... 2 6 ....................................................... 16 7 ......................................................... 7 8 ......................................................... 0 M-315 ................................................ 3 0 0 14 6 43 1 4 0 1 0 4 0 27 8 19 5 2 5 7 4 0 33 1 13 5 2 5 12 4 0 23 3 19 17 28 3 7 6 3 2 O. Lloyd May, USDA-ARS, 2200 Pocket Road, Florence, SC 29506-9706 PD 94056 ........................................... 7 PD 94063 ........................................... 4 PD 95034 ........................................... 0 PD 95079 ........................................... 7 ROWDEN ....................................... 33 PD 96001 ........................................... 5 PD 96005 ........................................... 1 PD 96024 ........................................... 1 PD 96031 ........................................... 2 M-315 ................................................ 0 2 6 5 0 16 4 0 6 9 0 3 6 0 2 12 9 4 4 8 3 5 2 4 3 40 9 8 2 5 3 4 5 2 3 25 6 3 3 6 2 Jack E. Jones, JaJo Genetics, 246 Maxine Drive, Baton Rouge, LA 70808-6831 JJ-1 ..................................................... 1 JJ-2 ..................................................... 3 JJ-3 ..................................................... 9 JJ-4 ..................................................... 0 ROWDEN ....................................... 26 JJ-5 ..................................................... 4 JJ-6 ..................................................... 0 JJ-7 ................................................... 13 JJ-8 ..................................................... 0 M-315 ................................................ 0 0 2 6 0 14 3 7 0 0 0 0 7 2 0 25 6 2 13 0 0 0 11 10 3 70 2 2 4 5 0 0 6 7 1 34 4 3 7 1 0 Gary Rea, Paymaster Cottonseed, RR 2, Box 60, Hale Center, TX 79041 1 ......................................................... 0 2 ......................................................... 1 3 ......................................................... 2 4 ......................................................... 1 ROWDEN ....................................... 34 5 ......................................................... 8 6 ......................................................... 2 7 ......................................................... 3 8 ......................................................... 5 M-315 ............................................... 3 0 1 3 9 20 1 0 2 0 0 13 6 1 0 22 3 0 0 0 3 7 6 0 3 22 0 3 0 3 0 5 4 1 3 24 3 1 1 2 1 continued 8 ALABAMA AGRICULTURAL EXPERIMENT STATION 1999 FUSARIUM WILT TEST, E. V. S MITH RESEARCH CENTER, TALLASSEE , A LABAMA Test entry designation 17 161 162 163 164 165 166 167 168 169 170 18 171 172 173 174 175 176 177 178 179 180 19 181 182 183 184 185 186 187 20 188 189 190 191 192 193 194 ——————Percent wilt by replication—————— 1 2 3 4 Mean W. P. Sappenfield, 115 Mango Cove, Leesburg, FL 34748 WPS-1 ................................................ 0 WPS-2 ................................................ 7 WPS-3 ................................................ 0 WPS-4 ................................................ 0 ROWDEN ....................................... 34 WPS-5 ................................................ 4 WPS-6 ................................................ 0 WPS-7 ................................................ 0 WPS-8 ................................................ 0 M-315 ................................................ 0 0 0 0 2 31 0 0 2 6 8 20 51 5 4 31 0 3 0 6 1 8 0 0 0 10 0 5 0 0 0 7 15 1 1 27 1 2 1 3 2 Michael Swindle, Paymaster Cottonseed, 2476 Hwy. 130 East, Stuttgart, AR 72160 MS-1 .................................................. 4 MS-2 .................................................. 2 MS-3 ................................................ 11 MS-4 ................................................ 19 ROWDEN ....................................... 46 MS-5 .................................................. 2 MS-6 .................................................. 7 MS-7 ................................................ 24 MS-8 .................................................. 4 M-315 ................................................ 0 7 14 20 2 44 4 0 6 4 0 20 12 17 7 22 0 16 5 0 0 3 3 2 3 5 0 2 8 0 3 8 8 12 8 29 1 6 11 2 1 Mark Barefield, Stoneville Pedigreed Seed Co., 2409 Commerce Lane, Albany, GA 31707 MB-1 ................................................ 28 MB-2 ................................................ 21 MB-3 ................................................ 10 MB-4 .................................................. 0 ROWDEN ....................................... 28 MB-5 ................................................ 40 MB-6 ................................................. 0 0 11 24 6 64 16 2 2 0 7 1 13 8 3 4 2 2 8 10 19 0 9 8 11 4 29 21 1 Lloyd McCall, Stoneville Pedigreed Seed Co., 2409 Commerce Lane, Albany, GA 31707 LM-1 .................................................. 0 LM-2 .................................................. 4 M-315 ................................................ 3 LM-3 .................................................. 0 LM-4 .................................................. 0 LM-5 .................................................. 6 LM-6 .................................................. 7 27 5 0 6 0 3 11 3 3 0 48 11 4 5 7 5 0 3 6 7 0 9 4 1 14 4 5 6 continued 1999 NATIONAL FUSARIUM WILT COTTON REPORT 9 1999 FUSARIUM WILT T EST, E. V. S MITH RESEARCH CENTER, T ALLASSEE , ALABAMA Test entry designation 21 195 196 197 198 199 200 201 202 22 203 204 205 206 207 208 209 23 210 211 212 213 214 215 216 217 24 218 219 220 221 222 223 224 225 25 226 227 228 229 230 ——————Percent wilt by replication—————— 1 2 3 4 Mean Steve Calhoun, Stoneville Pedigreed Seed Co., 2409 Commerce Lane, Albany, GA 31707 ROWDEN ....................................... 21 SC-1 ................................................. 13 SC-2 ................................................... 0 SC-3 ................................................... 3 SC-4 ................................................... 3 M-315 ................................................ 2 SC-5 ................................................... 4 SC-6 ................................................... 4 8 3 2 11 0 0 6 13 30 3 9 12 7 7 0 0 37 6 22 3 2 1 0 5 24 6 8 7 3 3 2 5 Randy Wood, Stoneville Pedigreed Seed Co., P.O. Box 569, Maricopa, AZ 85239 RW-1 ................................................. 2 RW-2 ................................................. 0 ROWDEN ....................................... 29 RW-3 ............................................... 10 RW-4 ................................................. 1 RW-5 ............................................... 14 RW-6 ................................................. 3 6 2 36 4 0 3 3 3 0 15 5 3 15 2 2 3 45 7 6 2 5 3 1 31 6 3 8 3 Mike Robinson, Stoneville Pedigreed Seed Co., 2409 Commerce Lane, Albany, GA 31707 M-315 ................................................ 0 MR-1 .................................................. 2 MR-2 .................................................. 9 MR-3 .................................................. 4 MR-4 .................................................. 5 ROWDEN ....................................... 41 MR-5 .................................................. 8 MR-6 .................................................. 8 0 2 6 10 15 39 3 57 2 0 6 12 19 35 0 15 2 2 4 6 0 6 2 11 1 1 6 8 10 30 4 23 Jefferson Gwyn, AgrEvo Cotton Seed Inter., 4301-A Highway 82 East, Greenville, MS 38701 ACSI EXP0052 ................................. 7 ACSI EXP0222 ............................... 12 M-315 ................................................ 0 ACSI EXP0223 ................................. 0 ACSI EXP0321 ............................... 10 ACSI EXP0781 ................................. 0 ACSI EXPIF1000 .............................. 2 ROWDEN ....................................... 19 22 3 0 6 4 3 1 26 9 9 0 10 10 13 0 25 1 3 3 0 3 0 0 9 10 7 1 4 7 4 1 20 Forest Robinson, USDA, Texas A & M University, College Station, TX 77843 AFR-1 ................................................ 5 AFR-2 ................................................ 6 AFR-3 ................................................ 4 AFR-4 ................................................ 4 M-315 ................................................ 0 15 4 18 15 3 5 3 9 6 0 8 3 4 1 3 8 4 9 7 1 continued 10 ALABAMA AGRICULTURAL EXPERIMENT STATION 1999 FUSARIUM WILT TEST, E. V. S MITH RESEARCH CENTER, TALLASSEE , A LABAMA Test entry designation 26 231 232 233 234 235 27 236 237 238 239 240 241 28 242 243 244 245 246 247 248 249 250 ——————Percent wilt by replication—————— 1 2 3 4 Mean Luther Bird, 729 Shady Lane, Bryan, TX 77802-4322 GP-2-37-96 ........................................ 0 GP-4-37-96 ...................................... 19 GP-6-19-96 ........................................ 5 GP 9529-1 ........................................ 15 ROWDEN ....................................... 49 0 5 7 0 52 0 0 0 0 22 6 17 0 0 20 2 10 3 4 36 Robert Humphries, Delta and Pine Land Co.,1305 North VIP Blvd., Casa Grande, AZ 85222 DPAZ-1 ............................................. 6 DPAZ-2 ........................................... 15 DPAZ-3 ............................................. 6 DPAZ-4 ........................................... 28 M-315 ................................................ 0 DPAZ-5 ............................................. 6 9 3 4 10 0 0 7 3 18 4 0 0 2 6 6 2 2 0 6 7 8 11 0 2 Kathryn M. Glass, Dept. of Agronomy and Soils, Auburn University, AL 36849-5412 Suregrow 125 BR ............................ 22 Suregrow 747 .................................. 24 Paymaster PM 1560 BG/RR ............. 0 ROWDEN ....................................... 29 Stoneville 474 .................................... 8 Deltapine NuCotn 33B ...................... 3 Deltapine DP 458 B/RR .................... 4 Deltapine Acala 90 ............................ 0 M-315 ................................................ 3 4 8 2 22 0 0 0 0 0 6 3 2 8 17 0 0 0 6 3 5 0 10 4 2 3 2 0 9 10 1 17 7 1 2 1 2 1999 NATIONAL FUSARIUM WILT COTTON REPORT 11