As.
.o-5-

°YM

t

3

'

Bulletin b f "1VRI May 1987 Alabama Agricultural Experiment Station Lowell T. Frobish, Director Auburn University Auburn University, Alabama

CONTENTS Page
MATERIALS AND METHODS RESULTS AND DISCUSSION ............................... ................................. .................... ....................

4
6

Farm er's Field, Elberta ................................. ................... 6 Plant Breeding Unit, Tallassee ......................................... 12 E. V. Smith Research Center, Shorter ............................. 16
SUM MARY .......................................................... REFERENCES ..................................................... ..................... 17 ......................

20

FIRST PRINTING 4M, MAY 1987

ON THE COVER: Response of different soybean cultivars on nematodeinfested field in Baldwin County, Alabama. Top to bottom: Braxon, Foster, Ransom, and Kirby.

Information contained herein is available to all without regard to race, color, sex, or national origin.

PERFORMANCE OF SOYBEAN CULTIVARS in Fields Infested with Plant-Parasitic Nematodes in Alabama'
R. RODRIGUEZ-KABANA, D.B. WEAVER, and E.L. CARDEN 2

T HE SOYBEAN [Glycine max (L.) Merr.] is subject to attack
by several species of nematodes (11). Economically important species in Alabama are the soybean cyst nematode (SCN) (Heteroderaglycines Ichinohe) and root-knot nematode (RKN), either the southern root-knot nematode [Meloidogyne incognita (Kofoid and White) Chitwood] or the peanut root-knot nematode [Meloidogyne arenaria(Neal) Chitwood]. Of lesser economic importance are the lance nematode [Hoplolaimusgaleatus (Cobb) Thorne], lesion nematode (Pratylenchus spp.), stubbyroot nematode [Paratrichodorus christie (Allen and Sidiqi)], and spiral nematode [Helicotylenchus dihystera (Steiner) Andrassy]. Recent surveys in Alabama indicate that 29 percent of the soybean fields are infested with SCN and 23 percent with RKN; 10 percent are infested with both species. Approximately 15 percent of Alabama's soybeans are lost annually to nematode feeding (7), about half to SCN and half to RKN and other nematodes. Previous studies have shown that yields of all soybean cultivars respond to effective nematicide treatments in soils heavily infested with RKN (3,6,8,12), but usually only susceptible cultivars respond to nematicide treatments in soils infested with SCN alone (1). The fumigant nematicides DBCP (1,2-dibromo-3-dichloropropane) and EDB (ethylene dibromide) are inexpensive, effective chemicals for nematode control on soybeans (6,12),
'This research was supported in part by a grant from Alabama Soybean Producers. 2Professor of Plant Pathology, Assistant Professor of Agronomy and Soils, and Superintendent of Gulf Coast Substation, respectively.

4

ALABAMA AGRICULTURAL EXPERIMENT STATION

but recent action by the Environmental Protection Agency has prohibited their use. Although effective nematicides are available for nematode control in soybeans (13), they are often not economical to use, especially when soybean prices fall below $6 per bushel. Soybean producers must therefore rely on cropping sequences (rotation with nonhost crops) and genetic resistance as their primary means of nematode control. Recent studies have shown the advantages of crop rotation (4,5) for controlling M. incognita when resistant soybean cultivars are used in the rotation. In addition, there has been a large increase in the number of soybean cultivars available during the past 15 years, particularly proprietary cultivars (2). Even though cultivar performance tests are conducted yearly in Alabama, they are not conducted in areas of heavy nematode infestation. Field experiments were conducted at three locations in Alabama with the following objectives: (1) determine the amount of recoverable soybean yield lost to nematodes in situations where more than one damaging species of nematodes are present, (2) determine relative resistance of soybean cultivars to mixed nematode populations, and (3) determine the effect of cultivar on final (harvest) nematode populations.
MATERIALS AND METHODS

A total of 19 field experiments was conducted during 198285 at three nematode-infested locations in Alabama. Seven cultivars were evaluated in each experiment with no nematicide (control) and 2 gallons per acre EDB applied at planting. The 14 treatments in each experiment were arranged in a 2 x 7 factorial structure in a randomized complete block design with eight replications. Plots were two rows 20 feet long endtrimmed at harvest to 16 feet, with spacings between rows of 30 to 40 inches depending on location. Planting dates ranged from May 15 to June 22. EDB was applied by two injectors 14 inches deep and 6 inches to each side of the row immediately before planting. Soil samples for nematode analysis were collected when plants were at or nearing maturity. Samples consisted of a composite of 16 to 20 soil cores (each 1.0 inch in diameter) from each plot from the root zone to a depth of 8 to 10 inches. A subsample of approximately 6 cubic inches (100 cubic centimeters) of soil was used to determine total nematode

PERFORMANCE OF SOYBEAN CULTIVARS
TABLE 1. MATURITY GROUP AND NEMATODE REACTIONS OF CULTIVARS AND GENETIC LINES EVALUATED WITH AND WITHOUT A NEMATICIDE IN FIELDS WITH VARIOUS NEMATODE POPULATIONS

5

Cultivar

Maturity M.i.'
............

Nematode reaction M.a. SCN (race 3) SCN (race 4)
S R R

Asgrow A5474

V

S2

Bedford ..................... .V Deltapine 105.............. V Epps ......................... V Forrest ...................... V Hartz 5370O ................ V Pioneer 9561l.............. V Pioneer 9571l.............. V VI Asgrow A6520O ............ Centennial .................. VI Davis ........................ GK67....................... VI Hartz 6383................. VI Jeff .......................... VI Leflore ...................... VI Northrup-King S69-96. VI

VI

Young....................... Asgrow A7372 ............ Braxton ..................... Coker 627 .................. Coker317 .................. Deltapine 417.............. Deltapine 497.............. GK 49....................... Gordon ..................... Hartz 7126 ................. Ring Around RA 702 .. Ransom ..................... Wright ...................... Cobb ........................ Coker 368 .................. Foster ....................... Johnston .................... Kirby ........................ Experimental genotypes Au82-2386 ................. F77-7142.................... F81-2815....................
F82-1739....................

Terra-Vig 606.............

VI VII VII VII VII VII VII VII VII VII VII VII VII VIII VIII VIII VIII VIII VII VII VII

VI

R S R R R R R S R S R R R R S
R

MR S MR

MR S

S MR S S S S S S S S
R

R S R R R R R R R S S R R R S
S

R S R S S S R R S S S S R R S
S

S R R R S R S S R S R S R R R R S R R R R
R

S S R S S S

MR S MR S
S S R S S S S R R R S
R

S S S R R S S S R R R S S S R R S R S R R
R

S S S S S S S S S S S S S S S S S S S S S
R

S R R Ga8O-1413.................. VII S S S N81-1756................... VII R R R F80-3602.................... VII 1Mi. = Meloidogyne incognita, M.a. = Meloidogyne arenaria, SCN nematode. 2R resistant, MR = moderately resistant, S = susceptible. R

VII

=

S S S soybean cyst

6

ALABAMA AGRICULTURAL EXPERIMENT STATION

numbers by the "salad bowl" incubation technique (9). Briefly, soil subsamples were wrapped in facial tissue paper (ScottiesŪ) and submerged in water for a 72-hour incubation period. The water was then passed through a 400-mesh sieve and nematodes were counted. The three locations were selected to represent certain nematode species and/or mixtures of species likely to be a problem in Alabama. Most of the experiments were conducted in a farmer's field in Baldwin County, near Elberta, on a Ruston loamy fine sand primarily infested with a mixture of RKN (M. incognita) and SCN (race 3). Experiments were also conducted at the Plant Breeding Unit, near Tallassee, on a Cahaba fine sandy loam, primarily infested with the "cotton complex" of nematodes: root knot (M. incognita), lance, stubby root nematodes, and lesion (P. brachyurus) nematodes. These species of nematodes tend to be present in fields that have a recent history of cotton production. The third location was at the E.V. Smith Research Center, near Shorter, on a Norfolk loamy sand primarily infested with SCN (race 3). Cultivars and experimental lines were selected based on known genetic resistance to particular nematode species, table 1, or were included as checks because of no known nematode resistance (i.e. Ransom). These lines represent a wide range of available commercial and elite experimental germplasm. With the exception of the application of EDB, recommended production practices were followed at each location for fertility and weed control. Insects were controlled as needed. No attempts were made to control foliar diseases; however, these diseases were not considered a yield-limiting factor in any of the experiments. All data were analyzed following standard procedures for analysis of variance (ANOVA) and differences between means were separated using Fisher's least significant difference. Unless otherwise indicated, differences reported in the text were significant at the 5 percent probability level. RESULTS AND DISCUSSION Farmer's Field, Elberta RKN larvae (M. incognita) occurred in large numbers in nonfumigated plots at Elberta in 1982, causing large yield losses, table 2. Smaller numbers of SCN (race 3) and stubby root nematodes were present. There was no interaction between nematicide treatment and cultivars for yield, indicating

PERFORMANCE OF SOYBEAN CULTIVARS
TABLE 2. SEED YIELDS AND NEMATODE NUMBERS AT THE R6 REPRODUCTIVE STAGE FOR SOYBEANS TREATED WITH 0 AND 2 GALLONS PER ACRE EDB AT ELBERTA, 1982

7

Cultivar

Seed yield/acre Control Fumigated

Bu. Bu. 5.3 27.5 A7372 ...... Coker 317 1.1 33.6 5.7 28.1 Agratech 67 30.1 RA 702 .... 7.9 33.8 Braxton .... 8.4 37.3 Foster ....... 12.2 36.5 Ransom .... 4.8 32.4 Mean ........ 6.6 5.6 LSD (0.05)

Nematodes/100 cc soil Stubby-root Root-knot larvae Soybean cyst larvae Control Fumigated Control Fumigated Control Fumigated No. No. No. No. No. No. 1 2 19 41 15 58 6 2 2 172 20 2 4 1 3 49 0 132 1 6 10 5 89 30 2 2 85 7 5 51 3 2 1 9 2 189 1 4 0 9 49 44 13 2 3 25 9 111 NS 3 58

that cultivars, regardless of their genetic resistance, responded similarly whether or not they were treated with nematicide. Plots that did not receive nematicide averaged 80 percent less yield than those treated with nematicide, and even the most resistant cultivar, Foster, suffered a yield loss of 67 percent. In 1983, with better rainfall, yields were higher in both fumigated and nonfumigated plots, table 3. Foster had significantly higher yields in nonfumigated plots than .other cultivars. Conditions favorable for high yields also favored large nematode populations. RKN again occurred in large numbers, with SCN and stubby-root nematodes present. RKN numbers were highest on Foster, the most resistant cultivar, but this was because of time of sampling. Sampling was done after nematode populations had started to decline due to plant
TABLE 3. SEED YIELDS AND NEMATODE NUMBERS AT THE R6 REPRODUCTIVE STAGE FOR SOYBEANS TREATED WITH 0 AND 2 GALLONS PER ACRE EDB AT ELBERTA, 1983

Cultivar

Seed yield/acre Control Fumigated

Bu. Bu. 16.5 38.2 A7372 ..... 18.7 54.7 Coker 317 38.2 Agratech 67 14.8 51.0 20.3 RA 702 .... 42.7 Braxton .... 22.4 56.6 Foster ....... 30.0 45.3 Ransom .... 16.1 46.7 Mean ........ 19.8 7.8 LSD (0.05)

Nematodes/100 cc soil Stubby-root Soybean cyst larvae Root-knot larvae Control Fumigated Control Fumigated Control Fumigated No. No. No. No. No. No. 3 15 26 26 11 312 6 7 5 4 383 60 4 11 22 16 36 338 26 8 1 10 93 393 9 7 17 44 69 213 7 19 3 2 454 37 5 7 9 24 153 364 11 5 9 22 26 110 7 17 134

8

ALABAMA AGRICULTURAL EXPERIMENT STATION
TABLE 4. SEED YIELDS AND NEMATODE NUMBERS AT THE R6 REPRODUCTIVE STAGE FOR SOYBEANS TREATED WITH 0 AND 2 GALLONS PER ACRE EDB AT

ELBERTA, 1983

Cultivar

Seed yield/acre Control Fumigated

Bu. Bu. Braxton .... 21.4 45.9 Cobb ....... 8.5 35.7 Coker 317 19.0 49.8 Foster ....... 26.1 51.1 Johnston ... 8.5 40.1 Kirby ........ 35.4 55.6 Ransom .... 14.5 41.5 Mean ........ 19.1 45.7 LSD (0.05) 7.1

Nematodes/100 cc soil Soybean cyst larvae Stubby-root Root-knot larvae Control Fumigated Control Fumigated Control Fumigated No. No. No. No. No. No. 86 34 12 22 2 6 58 45 6 26 1 9 220 77 1 1 1 9 27 1 4 1 5 276 0 5 143 33 30 33 216 12 1 12 1 13 110 34 1 14 3 9 158 37 7 16 1 8 55 18 6

death in the more susceptible cultivars, resulting in reduced populations on the susceptible ones. SCN populations tended to be higher in fumigated than in nonfumigated plots in both 1982 and 1983. This is probably due to the greater susceptibility of RKN to EDB, allowing better root growth and allowing SCN to compete more successfully for feeding sites in the fumigated plots. A second test was conducted on a different set of cultivars in 1983 with similar results, table 4. Seed yields and RKN numbers were high. Kirby, a nematoderesistant cultivar, had significantly higher seed yield than other cultivars in the nonfumigated plots. The interaction between cultivars and nematicide treatments for yield was not significant for either of the 1983 experiments. Yield reduction by nematode feeding was 58 percent (fumigated vs. nonfumiTABLE 5. SEED YIELDS AND NEMATODE NUMBERS AT THE R6 REPRODUCTIVE STAGE FOR SOYBEANS TREATED WITH 0 AND 2 GALLONS PER ACRE EDB AT

ELBERTA, 1984

Cultivar

Seed yield/acre Control Fumigated

Bu. Bu. Braxton .... 2.0 27.3 1.3 23.8 Cobb ........ 40.6 Coker 317 14.3 44.4 Foster ....... 25.6 30.3 Johnston ... 3.8 25.1 41.3 Kirby .... 29.1 Ransom .... 5.0 Mean ........ 11.0 33.8 5.8 LSD (0.05)

Nematodes/100 cc soil Soybean cyst larvae Stubby-root Root-knot larvae Control Fumigated Control Fumigated Control Fumigated No. No. No. No. No. No. 12 214 127 277 249 4 287 7 15 363 146 187 20 21 540 258 113 107 360 236 103 145 7 7 20 276 86 201 257 9 158 122 15 18 434 143 331 186 205 225 11 16 10 16 360 169 178 199 241 83 10

PERFORMANCE OF SOYBEAN CULTIVARS

9

gated) averaged for all cultivars across both experiments, but was only 36 percent for Kirby. Favorable rainfall resulted in high yields in fumigated plots again in 1984, tables 5, 6, and 7. For the first time, SCN larval numbers became a significant factor, with populations almost as high as those for RKN. As a result, cultivars with good genetic resistance to both RKN and SCN in some cases produced yields in nonfumigated plots that were equal to those of susceptible cultivars in fumigated plots. Nematode feeding resulted in an average 65 percent yield loss. Leflore, with a high level of resistance, had a yield loss of 44 percent. Kirby was included in all 1984 experiments as a nematode-resistant check based on 1983 results, and had losses of 39, 51, and 47 percent. Ransom, included as a nematode-susceptible check
TABLE 6. SEED YIELDS AND NEMATODE NUMBERS AT THE R6 REPRODUCTIVE STAGE FOR SOYBEANS TREATED WITH 0 AND 2 GALLONS PER ACRE EDB AT

ELBERTA, 1984 Cultivar Seed yield/acre Control Fumigated Nematodes/100 cc soil Root-knot larvae Soybean cyst larvae Stubby-root Control Fumigated Control Fumigated Control Fumigated

Bu. Coker 368 ..... 14.5 Forrest .......... 10.9 Gordon ......... 13.9 Kirby ............. 19.4 Leflore . 25.4 Ransom ......... 6.7 Terra-Vig 606 9.7 Mean ............. 14.4
LSD (0.05) .... 6.1

Bu. 43.6 35.1 36.9 39.3 45.4 29.7 34.5 37.8

No. 247 255 157 196 563 215 243 268
137

No. 104 113 132 128 182 103 193 136

No. 196 149 131 170 58 202 200 150
86

No. 186 141 213 135 184 360 334 222

No. 11 10 8 4 9 8 8 8
11

No. 9 12 10 11 18 8 8 11

TABLE 7. SEED YIELDS AND NEMATODE NUMBERS AT THE R6 REPRODUCTIVE STAGE FOR SOYBEANS TREATED WITH 0 AND 2 GALLONS PER ACRE EDB AT ELBERTA, 1984

Cultivar

Seed yield/acre Control Fumigated

Nematodes/100 cc soil Soybean cyst larvae Stubby-root Root-knot larvae Control Fumigated Control Fumigated Control Fumigated

Bu. Bu. Braxton .... 10.3 26.6 F77-7142 .. 16.9 41.1 Foster ....... 20.6 41.1 Kirby ........ 19.4 36.9 N81-1756 4.2 24.2 26.0 Ransom .... 4.8 S69-96 ...... 3.0 30.3 11.3 32.3 Mean ........ LSD (0.05) 6.8

No. 169 207 472 267 385 272 252 289 132

No. 62 81 151 87 79 177 144 112

No. 218 149 137 153 163 173 125 160 103

No. 290 203 83 142 301 229 235 212

No. 9 10 5 5 3 8 6 7 9

No. 10 10 12 11 11 13 10 11

10

ALABAMA AGRICULTURAL EXPERIMENT STATION
TABLE 8. SEED YIELDS AND NEMATODE NUMBERS AT THE R6 REPRODUCTIVE

STAGE FOR SOYBEANS TREATED WITH 0 AND 2 GALLONS PER ACRE EDB AT ELBERTA, 1985

CultivarControl Fumigated Bu. Coker 368 ..... 10.2 Forrest ....... 7.5 Gordon ......... 8.8 Kirby ............. 14.3 Leflore .... 17.7 Ransom ......... 7.5 Terra-Vig 606 4.8 Mean ............. 10.1
LSD (0.05) .... 4.7

Seed yield/acre

Nematodes/100 cc soil

Bu. 25.1 14.3 19.7 21.8 29.9 15.6 15.0 20.2

Root-knot larvae Soybean cyst larvae Stubby-root Control Fumigated Control Fumigated Control Fumigated No. No. No. No. No. No. 112 10 58 108 22 8 25 28 27 30 13 12 49 22 48 51 14 12 53 18 45 72 14 12 220 29 14 18 16 4 75 20 58 98 10 11 43 19 59 80 15 11 82 21 44 65 15 10
51 40 11

based on 1982 and 1983 results, had losses of 83, 77, and 82 percent. As in the two previous years, larval populations of SCN tended to increase in response to fumigation while those of RKN were reduced. Cultivar x fumigation treatment interactions were absent in 1985, except for the experiment summarized in table 6, which had a significant cultivar x fumigation treatment interaction for SCN numbers. Some cultivars showed no response to fumigation, while others showed larger SCN numbers in fumigated than control plots. The shift toward dominance of SCN continued in 1985, tables 8, 9, 10, 11, 12, and 13, especially with cultivars that had no SCN resistance. Cultivars with SCN resistance tended to have more RKN than SCN larvae in nonfumigated plots, and more SCN than RKN in fumigated plots. SCN were
TABLE 9. SEED YIELDS AND NEMATODE NUMBERS AT THE R6 REPRODUCTIVE STAGE FOR SOYBEANS TREATED WITH 0 AND 2 GALLONS PER ACRE EDB AT ELBERTA, 1985

Cultivar

Control Fumigated

Seed yield/acre

Nematodes/100 cc soil
Root-knot larvae Soybean cyst larvae Stubby-root

Bu. A5474 .......... 10.2 Bedford ......... 12.9 Deltapine 105 4.1 Epps .............. 5.4 Hartz 5370... 10.2 Kirby ............ 10.2 Ransom ......... 3.4 Mean ............. 8.1
LSD (0.05) .... 3.4

Bu. 23.1 19.7 15.0 19.7 22.4 22.4 19.0 20.2

Control Fumigated Control Fumigated Control Fumigated No. No. No. No. No. No. 80 35 13 23 2 5 88 14 23 9 6 3 24 13 17 32 2 3 53 25 12 22 4 1 45 18 9 12 1 5 35 12 47 50 2 3 69 27 32 78 2 4 56 21 22 28 3 3
35 26 N.S.

PERFORMANCE

OF

SOYBEAN

CULTIVARS

11

TABLE 10. SEED YIELDS AND NEMATODE NUMBERS AT THE R6 REPRODUCTIVE STAGE FOR SOYBEANS TREATED WITH 0 AND 2 GALLONS PER ACRE EDB AT

ELBERTA, 1985 Seed yield/acre Control Fumigated Nematodes/100 cc soil Stubby-root Soybean cyst larvae Root-knot larvae Control Fumigated Control Fumigated Control Fumigated

Cultivar

Bu. Bu. 4.8 A7372 ...... 0.7 4.8 .0 Agratech 67 29.2 Coker 317 8.8 Braxton .... .7 4.1 19.0 Foster ....... 4.8 4.1 22.4 RA 702 .... 17.0 Ransom .... 2.0 3.0 14.5 Mean ........ 3.2 LSD (0.05)

No. 59 62 212 44 89 90 92 93 62

No. 12 5 71 16 10 25 25 23

No. 47 47 82 46 49 54 77 57 61

No. 164 134 139 72 98 101 164 125

No. 8 4 13 9 13 21 15 12 15

No. 20 29 23 23 18 9 18 20

TABLE 11. SEED YIELDS AND NEMATODE NUMBERS AT THE R6 REPRODUCTIVE STAGE FOR SOYBEANS TREATED WITH 0 AND 2 GALLONS PER ACRE EDB AT ELBERTA, 1985 Cultivar Seed yield/acre Control Fumigated Nematodes/100 cc soil Stubby-root Soybean cyst larvae Root-knot larvae Control Fumigated Control Fumigated Control Fumigated

Bu. Bu. 0.7 2.7 Braxton .... 3.4 .7 Cobb ........ 29.2 10.2 Coker 317 22.4 Foster ....... 8.2 11.6 1.4 Johnston ... 23.8 Kirby ........ 12.2 15.6 4.1 Ransom .. 5.4 15.5 Mean ........ 3.2 LSD (0.05)

No. 13 28 198 146 37 101 76 86 53

No. 4 14 26 5 21 21 17 15

No. 67 49 27 36 69 44 59 50 51

No. 51 187 43 42 112 68 102 86

No. 2 3 2 3 2 1 3 2 N.S.

No. 7 3 4 3 4 2 4 4

TABLE 12. SEED YIELDS AND NEMATODE NUMBERS AT THE R6 REPRODUCTIVE STAGE FOR SOYBEANS TREATED WITH 0 AND 2 GALLONS PER ACRE EDB AT ELBERTA, 1985 Seed yield/acre Control Fumigated Nematodes/100 cc soil Stubby-root Soybean cyst larvae Root-knot larvae Control Fumigated Control Fumigated Control Fumigated

Cultivar

Bu. 3.4 Centennial..... 4.1 Coker 627 ..... Hartz 6383 ... 8.8 6.1 Jeff ............... Kirby ......... 8.2 10.2 Pioneer 9571 3.4 Ransom ......... Mean ............ 6.3 3.8 LSD (0.05) ....

Bu. 21.1 21.1 18.4 21.1 18.4 15.6 19.7 19.3

No. 41 69 35 68 44 66 48 53 28

No. 7 9 9 9 9 8 18 10

No. 57 32 43 10 61 3 47 36 26

No. 42 35 43 11 81 11 119 47

No. 11 3 0 4 8 3 5 5 N.S.

No. 3 8 7 4 6 7 4 6

12

ALABAMA AGRICULTURAL EXPERIMENT STATION

TABLE 13. SEED YIELDS AND NEMATODE NUMBERS AT THE R6 REPRODUCTIVE STAGE FOR SOYBEANS TREATED WITH 0 AND 2 GALLONS PER ACRE EDB AT

ELBERTA,

1985

Cultivar

Seed yield/acre Control Fumigated

Bu. Bu. Au82-2386 4.1 15.0 F80-3602 .. 11.6 23.8 F81-2815 .. 6.8 21.8 F82-1739 .. 15.6 27.9 Ga80-1413 9.5 24.5 Kirby ........ 12.2 23.1 Ransom .. 7.5 17.7 Mean ........ 9.6 22.0 LSD (0..05) 4.7

Nematodes/100 cc soil Root-knot larvae Soybean cyst larvae Stubby-root Control Fumigated Control Fumigated Control Fumigated No. No. No. No. No. No. 34 3 42 119 2 8 52 15 54 105 7 10 99 9 46 79 0 2 107 13 20 28 2 9 106 21 23 51 4 5 31 6 81 85 0 3 55 8 53 87 1 2 69 11 47 79 2 6 42 38 6

apparently able to increase due to reduced competition from RKN in fumigated plots. There was also evidence of a shift in SCN population from race 3 to race 4. Cultivars with resistance to both SCN races, such as Leflore, Jeff, and Pioneer 9571, tended to have significantly lower numbers of SCN larvae than cultivars with resistance to SCN race 3 alone, tables 8 and 12. This greater SCN resistance did not result in significantly greater yield in nonfumigated plots, although Leflore tended to yield more. Interactions between cultivars and fumigation were much more prevalent in 1985 than in previous years. Significant interactions were found for RKN numbers, tables 8 and 11, SCN numbers, tables 11 and 12, and yield, tables 9, 10, 11, and 12. This was probably due to the increase in SCN numbers; no other variable was so markedly different from the previous years. While these interaction effects were significant, magnitude of the main effects (cultivars and fumigation treatments) mean squares was in most cases much larger than the magnitude of mean squares for the interactions, indicating that the main effects were relatively more important. Plant Breeding Unit, Tallassee In 1983 at Tallassee, lance, stubby-root, and spiral nematodes were present in the largest numbers, and lesion nematodes were also present, table 14. Fumigation did not decrease stubby-root nematodes, but significantly reduced lance and spiral nematodes. Even though yields were good in the control plots (31.5 bushels per acre), this was only 76 percent of the yield that was possible when nematodes were controlled

-U TABLE 14. SEED YIELDS AND NEMATODE NUMBERS AT THE R6 REPRODUCTIVE STAGE FOR SOYBEANS GALLONS PER ACRE EDB AT PLANT BREEDING UNIT, 1983 TREATED WITH 0 AND 2

0 Spiral Fumigated No. 5 2 5 7 8
2

Seed yield/acreNematodes/100ccsoil Cultivar Seedtld/aedLance Control Fumigated No. Bu. Bu. No. 12 Braxton ......... 34.2 44.4 22 Centennial.... 33.4 42.7 31 5 31.6 42.9 29 14 Cobb ............. 31.9 42.1 22 14 Davis........... 9 Forrest .......... 26.2 37.3 27 5 Kirby..........30.8 40.5 22 Ransom ......... 32.3 41.9 15 8 Mean ........... 31.5 41.7 24 10
LSD (0.05) ....
TABLE 15.

Lesion Control Fumigated No. No. 7 2 6 1 11 4 4 6 8 6 9 3 7 1 8 3
6

Stubby-root Control Fumigated No. No. 45 27 25 28 41 48 21 20 18 22
35 30

Control No. 31 20 22 19 15
26

z
0 0

rn

m

z
C c-

33 32 17

42 31

17 21 11

4 5

4.2

13

SEED YIELDS AND NEMATODE NUMBERS AT THE R6 REPRODUCTIVE STAGE FOR SOYBEANS GALLONS PER ACRE EDB AT PLANT BREEDING UNIT, 1984

TREATED WITH 0 AND 2

Cultivar

Seed yield/acre Control Fumigated Bu. 50.3 47.6 46.2 46.2 38.1 41.5 46.2 45.2 4.3

Lance Control Fumigated No. 22 29 22 17 24 13 9 19 12 No. 3 4 3 6 2 4 0 3

Nematodes/100 cc soil Lesion Stubby-root Control Fumigated Control Fumigated No. 11 18 6 6 10 11 14 11 7 No. 5 3 1 2 0 3 1 2 No. 21 19 27 21 16 22 14 20 18 No. 43 26 51 32 24 22 19 31

Spiral Control Fumigated No. 17 17 24 28 4 16 13 17 12
CA)

Bu. Braxton........ 42.2 Centennial.. 38.8 Cobb........... 40.8 Davis........... 42.2 Forrest......... 29.2 Kirby........... 36.0 Ransom........ 42.8 Mean........... 38.9 LSD (0.05) ....

No. 2 1 3 5 1 2 0 2

14

ALABAMA

AGRICULTURAL

EXPERIMENT

STATION

(41.7 bushels per acre in fumigated plots). Because population development of stubby-root nematodes was not controlled by fumigation, lance and/or spiral nematodes appeared to be the yield-limiting species in this environment. Consistent yield response of the cultivars to fumigation indicates little difference among cultivars for genetic resistance to lance or spiral nematodes. Results were similar in 1984, table 15. Yields were generally higher and fumigation resulted in only a 14 percent yield increase, but nematode populations were similar to 1983 for all species. In 1985, spiral nematode numbers were insignificant, while lance and stubby-root nematodes continued to be the dominant species, table 16. Feeding by these species resulted in an average yield loss of 13 percent.
TABLE 16. SEED YIELDS AND NEMATODE NUMBERS AT THE R6 REPRODUCTIVE STAGE FOR SOYBEANS TREATED WITH 0 AND 2 GALLONS PER ACRE EDB AT PLANT BREEDING UNIT, 1985 Seed yield/acre Control Fumigated Nematodes/100 cc soil Stubby-root Lance Lesion Control Fumigated Control Fumigated Control Fumigated

Cultivar

Bu. Bu. 41.5 Braxton .... 37.4 36.7 43.5 Centennial 39.4 46.9 Cobb ......... 40.1 Davis ......... 38.1 Forrest ...... 37.4 44.9 Kirby ........ 35.4 38.8 44.2 Ransom .... 36.0 42.8 Mean ........ 37.2 3.8 LSD (0.05)

No. 18 26 15 18 23 11 12 18 8

No. 3 6 4 8 2 2 2 4

No. 7 1 5 13 3 5 5 6 4

No. 0 1 1 1 1 3 0 2 1

No. 10 20 14 24 16 15 11 16 9

No. 9 16 19 22 10 15 15 15

Two additional tests were conducted in a different field site at the Plant Breeding Unit in 1985. RKN (M. incognita) predominated at this site, along with significant numbers of lesion, stubby-root, and spiral nematodes, tables 17 and 18. Even though RKN numbers were relatively high, nematode feeding caused only about a 6 percent yield loss. With the exception of Leflore, cultivars were consistent in response to fumigation.

TABLE 17. SEED YIELDS AND NEMATODE NUMBERS AT THE R6 REPRODUCTIVE STAGE FOR SOYBEANS GALLONS PER ACRE EDB AT PLANT BREEDING UNIT, 1985

TREATED WITH 0 AND 2

m
I1

0 Spiral Fumigated No. 7 3 5 4 4 0 2 4 6

Cultivar

Seed yield/acre Control Fumigated Bu. 49.6 45.6 46.9 41.5 55.1 41.5 42.8 46.1 6.3 Bu. 51.7 49.0 49.0 45.6 55.1 45.6 46.2 48.9

Braxton ......... Gordon ......... Hartz 6383 ... Jeff........... Leflore .......... Wright .......... Young.......... Mean ........... LSD (0.05) ....

Root-knot larvae Control Fumigated No. No. 1 53 63 8 63 5 30 6 31 5 149 17 66 11 65 8 23

Nematodes/l100 cc soil Lesion Stubby-root Control Fumigated Control Fumigated No. No. No. No. 12 4 23 13 8 12 4 11 18 1 11 5 25 1 14 4 6 9 4 0 9 6 9 3 19 6 6 5 14 4 11 5 8 6

z
0

Control No. 3 9 10 9 4 4 3 6

0)

m

m

z
C
c-

TABLE 18. SEED YIELDS AND NEMATODE NUMBERS AT THE R6 REPRODUCTIVE STAGE FOR SOYBEANS TREATED WITH 0 AND 2 GALLONS PER ACRE EDB AT PLANT BREEDING UNIT, 1985

Cultivar

Seed yield/acre Control Fumigated Bu. 39.4 46.2 48.3 38.8 44.2 48.6 32.0 42.5 4.4

Bu. Asgrow A7372 40.1 Braxton........ 44.2 Coker 627 .. 41.5 Deltapine 417 35.4 Deltapine 497 40.8 Hartz 7126.. 45.6 RA 702........ 32.0 Mean........... 39.9 LSD (0.05) ....

RotkolaveSub-ot Control Fumigated No. No. 16 11 12 9 6 4 8 0 36 5 44 7 11 1 19 5 14

Nematodes/100 cc soil _ pil Control Fumigated Control Fumigated No. No. No. No. 17 11 16 5 43 14 20 15 30 22 20 1 19 9 11 1 37 22 21 6 29 3 15 3 17 15 4 5 27 14 15 5 14 10

Lance Control Fumigated No. No. 21 9 37 5 20 6 18 9 37 16 31 5 19 4 26 8 12
CA'

16

ALABAMA

AGRICULTURAL

EXPERIMENT

STATION

E. V. Smith Research Center, Shorter

Because it was known prior to planting that SCN was the primary nematode species at this location, only cultivars with genetic resistance to SCN were planted, with the exception of Braxton which was included as an SCN-susceptible check. This had the effect of reducing SCN larval numbers in all plots except Braxton, tables 19 and 20. Fumigation with EDB had no effect on SCN numbers in either experiment. Response to fumigation was fairly consistent for resistant cultivars in the first experiment, table 19, generally about 8 bushels per acre. The exception was Leflore, which showed no response to fumigation; however, the cultivar x fumigation interaction was not significant for any of the variables in both experiments. Braxton did not respond to fumigation in one of the experTABLE 19. SEED YIELDS AND NEMATODE NUMBERS AT THE R6 REPRODUCTIVE STAGE FOR SOYBEANS TREATED WITH 0 AND 2 GALLONS PER ACRE EDB AT E. V. SMITH RESEARCH CENTER, 1985

Nematodes/100 cc soil Seed yield/acre Lesion Stubby-root Control Fumigated Soybean cyst larvae Control Fumigated Control Fumigated Control Fumigated No. No. No. No. No. Bu. No. Bu. 1 0 11 11 8 19 43.5 Asgrow A5474 32.0 3 1 13 7 14 10 34.0 Bedford ......... 25.8 1 11 12 9 22 57 14.3 Braxton ......... 14.3 1 3 9 9 11 5 33.3 Epps ........ . 24.5 1 1 17 12 6 2 35.4 Forrest .......... 27.9 5 0 9 15 8 27.9 8 Leflore .......... 25.2 4 1 7 10 2 10 37.4 26.5 Pioneer 9561 4 1 11 17 12 8 32.3 Mean ............ 25.2 Cultivar
LSD (0.05) .... 6.5 21 N.S. 6

TABLE 20. SEED YIELDS AND NEMATODE NUMBERS AT THE R6 REPRODUCTIVE STAGE FOR SOYBEANS TREATED WITH 0 AND 2 GALLONS PER ACRE EDB AT E. V. SMITH RESEARCH CENTER, 1985

Seed yield/acre Control Fumigated Soybean Control Bu. No. Bu. 3 31.3 27.2 Asgrow A652 ....... 31 21.1 14.3 Braxton .. .......... 9 35.4 26.5 Centennial ............ 8 35.4 30.6 Gordon ................ 3 32.0 37.4 Hartz 6383 ........... 14 35.4 27.2 Hartz 7126 ........... 1 29.2 30.6 Jeff ............ ............ 10 32.2 26.9 ........... Mean ... 6.3 LSD (0.05) ............ Cultivar

Nematodes/100 cc soil Stubby-root cyst larvae Fumigated Control Fumigated No. No. No. 3 11 3 6 5 28 8 4 8 5 6 0 10 10 0 4 15 1 3 11 3 6 6 9 N.S. 19

PERFORMANCE

OF

SOYBEAN

CULTIVARS

17

iments, table 19, but showed a large response in the other, table 20. Response of resistant cultivars was consistent, but somewhat less in the second experiment than in the first. Previous research has shown that some SCN-resistant cultivars do not respond to nematicide in SCN-infested soil (1). The fact that Leflore and Jeff, cultivars with resistance to both SCN races 3 and 4, did not respond to nematicide might indicate the presence of SCN race 4, but other SCN race 3 and 4-resistant cultivars (A5474, Bedford, and Epps) showed large yield responses to nematicide. The possibility that this field could be infested with race 5 is unlikely because this was the first year SCN-resistant soybeans were grown. SUMMARY The three test locations represent very different situations with regards to endemic nematode populations. Elberta has probably the worst situation, where both RKN and SCN occur in large numbers. Economic yields probably cannot be made under these circumstances without a nematicide even with the most resistant cultivar. However, the effects of the nematicide and genetic resistance were generally additive, as evidenced by the general lack of a significant cultivar x nematicide treatment interaction for seed yield. Highest yields were generally obtained with cultivars that had the broadest spectrum of nematode resistance, regardless of nematicide treatment. For example, Leflore, which has resistance to both species of RKN as well as to SCN races 3 and 4, was the high-yielding cultivar in both fumigated and nonfumigated plots in 1984 and 1985, tables 6 and 8. Kirby and Foster, other cultivars with resistance to multiple nematode species, also performed well. Braxton and Cobb, however, which have resistance only to RKN, did not yield more than Ransom, which has no nematode resistance, tables 5, 10, and 11. Use of RKN-resistant cultivars did not result in lower RKN numbers at harvest; in fact, the opposite occurred. Leflore, Foster, and Kirby tended to support RKN populations as high or higher than RKN-susceptible cultivars. This can be partially explained by the fact that in nonfumigated plots, nematode feeding often caused RKN-susceptible cultivars to die well before normal harvest dates. Thus when the plots were sampled, nematode populations in these plots had already started

18

ALABAMA

AGRICULTURAL

EXPERIMENT

STATION

to decline. However, RKN-resistant cultivars continued to grow, produce roots, and supported ever-increasing RKN populations. It had been previously shown (10) that RKN populations developed more slowly on resistant cultivars, but such varieties were able to support as many or more RKN as susceptible cultivars. Thus, use of RKN-resistant cultivars is not a good strategy for reducing RKN numbers. SCN, which occurred in low numbers in 1982 and increased somewhat in 1983, became a major problem in 1984. Fumigation generally increased SCN numbers due to a combination of less susceptibility of SCN to EDB, more root growth in EDB-treated plots, and less competition from RKN. SCNresistant cultivars usually had significantly lower SCN numbers than SCN-susceptible cultivars, especially in fumigated plots. Again, SCN numbers on susceptible cultivars were probably not as high at sampling as they were at their peak because of early plant death in many plots. At the Plant Breeding Unit, where SCN did not occur, yields on nonfumigated plots were good while yield response to fumigation was relatively small. In nonfumigated plots, cultivars tended to yield similarly regardless of nematode resistance, except for Forrest, which probably yielded less because of its earlier (maturity group V) maturity. Thus, choice of cultivar did not influence performance at that location nearly so much as at Elberta. Although use of a nematicide may not be economically feasible in this situation, in almost every case cultivars responded positively to fumigation (average increase = 6 bushels per acre). Nematodes did not occur in high numbers, and plants in all plots appeared to be healthy with no symptoms of nematode damage. Yet, nematode feeding reduced yields an average 5.5 bushels per acre across experiments and years compared to fumigated plots. This demonstrates that soybeans that appear to be healthy can still suffer significant "hidden" yield losses from nematode damage. At the E. V. Smith Research Center, resistant cultivars yielded well and reduced SCN numbers significantly in nonfumigated plots compared to susceptible Braxton, and fumigation resulted in an average yield response of 6.6 bushels per acre for the resistant cultivars. Susceptible Braxton averaged only a 3.4-bushel per acre response to EDB fumigation, indicating that it is not always possible to grow a susceptible

PERFORMANCE

OF

SOYBEAN

CULTIVARS

PERFORMANCE OF SOYBEAN CULTIVARS

19 1

variety and attempt to control soybean cyst nematodes with chemicals. Several conclusions can be drawn from these results: 1. Where large populations of RKN and SCN exist together, highest yields are possible only by growing a cultivar with a broad spectrum of nematode resistance together with using an effective nematicide. In general, planting a susceptible cultivar and using a nematicide is no better than planting a resistant cultivar. 2. Where RKN and other plant-parasitic nematode species exist in low numbers, plants can often suffer yield loss without visible symptoms of nematode feeding. Inclusion of a nematicide or other nematode control measure may increase yields, but economics of nematode control will largely be dictated by crop value. 3. Where SCN is the primary nematode species, use of resistant cultivars is an effective method of nematode control, and small additional yield gains can be realized by applying an effective nematicide. Again, economics of nematicide usage in this situation will be dictated by crop value. These experiments have examined only two aspects of plantparasitic nematode management in soybean: resistant varieties and nematicide use. The nematicide used in these experiments (EDB) has been banned by EPA action. Other alternative nematicides may or may not provide the same degree of control. Inclusion of management practices, particularly rotation with nonhost crops, and for SCN, rotations involving susceptible and resistant varieties to stabilize populations, may enhance productivity.

(1)

(2) (3) (4) (5)

REFERENCES Epps, J.M., L.D. YOUNG, AND E.E. HARTWIG. 1981. Evaluation of Nematicides and Resistant Cultivar for Control of Soybean Cyst Nematode Race 4. Plant Dis. 65:665-666. JOHNSON, W.C., D.L. THURLOW, AND D. WILLIAMS. 1986. Performance of Soybean Varieties in Alabama. 1985. Agronomy and Soils Dept. Ser. No. 108. Ala. Agr. Exp. Sta. KINLOCH, R.A. 1974. Response of Soybean Cultivars to Nematicide Treatments of Soil Infested with Meloidogyne incognita. J. Nematol. 6:7-11. . 1983. Influence of Maize Rotations on the Yield of Soybeans Grown in Meloidogyne incognita Infested Soil. J. Nematol. 15:398-405. AND T.D. HEWLETT. 1984. Economic Analyses of Monocultured and Maize-rotated Soybean Crops Grown on Southern Root-knot Nematode Infested Soil. Soil and Crop Sci. Soc. Fla. Proc. 43:172-174.
MINTON, N.A., M.B. PARKER, O.L. BROOKS, AND C.E. PERRY. 1976.

(6)

(7)
(8) (9)

Evaluation of Nematicides for Control of Nematodes in Soybeans. Ga. Agr. Exp. Sta. Res. Bull. 189. MULROONEY, R.P. 1985. Soybean Disease Loss Estimate for Southern United States in 198g. Plant Dis. 69:92.
RODRIGUEZ-KABANA, R. AND D.L. THURLOW. 1980. Evaluation of

(10)
(11)
(12)

Selected Soybean Cultivars in a Field Infested with Meloidogyne arenaria and Heterodera glycines. Nematropica 10:50-55. AND M.H. POPE. 1981. A Simple Method for the Extraction of Nematodes from Soil. Nematropica 11:175-176. AND D.B. WEAVER. 1984. Soybean Cultivars and Development of Populations of Meloidogyne incognita in Soil. Nematropica 14:46-56. SCHMIDTT, D.P. 1985. Plant-parasitic Nematodes Associated with Soybeans. pp. 541-546. In R.M. Shibles, (ed.). World Soybean Res. Conf. III Proc. Westview Press, Boulder, Colo.
WEAVER, D.B., R. RODRIGUEZ-KABANA, AND D.G. ROBERTSON. 1985.

(13)

Performance of Selected Soybean Cultivars in a Field Infested with Mixtures of Root-knot, Soybean Cyst, and Other Phytonematodes. Agron. J. 77:249-253. ZIRAKPARVAR, M.E. 1985. Chemical Control of Nematodes Attacking Soybeans. pp. 523-527. In R.M. Shibles (ed.). World Soybean Res. Conf. III Proc. Westview Press, Boulder, Colo.