r-r'JLiY' 9FAiCLI:A ELA~k r41.' GY ''LL f1 r i A' ~ . ~k '4, DIRECTORS COMMENTS N THIS ISSUE Tillage Practice Helps Avert Soil Compaction Problems_3 The announcement recently by Presi- System Visually AssessesMPine Seedlig Qality 4 dent Muse of the establishment of a Public "Opinons Offered on Private Forests'}__ _ _ 5 Food Technology Institute at Auburn , , y, w 4provides many challenges and opportunities Immune StatuA R KIJ" t~f'erer .s'St-udi.d 6 Immue Satuof KU uffeersStuied_________________ 6 for those of us in the Experiment Station. 1PM Strategy Effectively Controls Smokybrbwn Cockroaches 7 The Food Technology Institute (FF1) is Granular an association of scientists from the Colleges GranlarInsctiideAC n ga 'm BeefiialInsctsii eants aJof Agriculture, Engineering, Sciences and Strawberry Variety 'T'ests Identify Best Berries9 M a a V M c a Solid Waste Becoming aNeResource___ 10 the School of Human Sciences. The FI Dobl idwllCu~aisReuc ne~ Ue nBrierHoss'_ povides opportunities for research to im- Double Sidea sr l a douses~1IEweC~C'OUe 11 p prove food safety, develop value added Disease Transmission ia Ebband Flow Systems Analyzed 12 Nitrogen Rates and Plant pacing Affect Collard Production 13 ties, improve marketing strategies for food Advetisng ncrase th Sae o Liv Cafis ______________ 14 producers, and provide technical support Advertising Increae tleS e of iveiCa fie r 14 for developing new food-related industries Rotating Soybean Cultivars MainainsYigelds ______ 15 Which Chiggers Attack Humans in Alabama? 16 In announcing establishment of the Ff1, Dr. Muse noted that it has more potential to ON THE COVER. Business is booming at Auburn University's fee-fishing pond and positively impact on the State's economy live-fish market, especially after an advertising campaign that has increased than any program currently underway at sales up to 50%, see story on page 14. Auburn University. By developing new food industries that better utilize cornmodi- ties produced in Alabama, the FTI can create U P C 0 M I N G E V E N T S many new jobs and generate millions of additional dollars of new income annually. June 23 Horticulture Field Day, E.V. Smith Research, Shorter As the primary research component of July 1 Fruit and Vegetable Field Day, Chilton Area Horticulture Substation, the FTI, we in the Experiment Station are Clanton excited about the opportunities this new or- July 13-16 American Peanut Research and Education Society Meeting, Huntsville ganization provides. It provides an ideal July 22-24 Alabama Farmers Federation Commodity Conference, Birmingham foufrreachndxtsinom - July 29 Cotton Field Day, Tennessee Valley Substation, Belle Minafoufrreachndxtsi -om - August 4 Agronomy Field Day, E.V. Smith Research Center, Shorter nents of a land grant university like Au- August 12 Field Crops Field Day, Prattville Experiment Field burn-to work together. The ultimate ben- August 17 All Commodity Field Day, Sand Mountain Substation, Crossville eficiary of the FTI will be all the people of August 19 Horticulture Field Day, Piedmont Substation, Camp Hill Alabama. S um m er 1 9 93 V o lu me 4 0 N u mb er 2 A quarterly report of research published by the Alabama Agricultural Experiment Station, Auburn University. LOWELL T. FROBISH ..... Director DAVID H. TEEM ............. Associate Director RUSSELL B. MUINTIFERING ... Associate Director PAT GREEN .................. Assistant Director ROY ROBERSON ............ Assistant Director KATIE SMITH ............... Associate Editor ROBYN HEARN .............. Associate Editor TERESA RODRIGUEZ ..... Art Designer Editorial Committee: Lowell T. Frobish; D.G. Himelrick ,Associate Professor of Horticulture; K.L. Bowen, Assistant Professor of Plant Pathology; E.T. Moran, Professor of Poultry Science; L.A. Shanley, AssistantProfessor of ConsumerAffairs; C.W. Wood, Assistant Professor of Agronomy and Soils; H.W. Kinnucan, Associate Professor of Agricultural Eco- nomics and Rural Sociology; R.A. Tufts, Associate Professor of Forestry; W.J. Moar, Assistant Professor of Entomology; J.L. Sartin, Associate Professor of Animal Health Research; and Roy Roberson. EDITOR'S NOTE. Mention of trade names does not indicate endorsement by the Alabama Agricultural Experiment Station or Auburn University of one brand over another. Any use of pesticide rates in excess of labeled amounts in research reported does not constitute recommendation of such rate. Such use is simply part of the scientific investigation necessary to evaluate various materials. No chemi- cal should be used at rates above those permitted by the label. Information contained herein is available to all persons without regard to race, color, sex, or national origin. R.L. RAPER, D.W. REEVES, E.C. BURT, AND H.A. TORBERT A CONSERVATION TILLAGE PRACTIC] THAT RESISTS COMPACTION[ / U ncontrolled field traffic can cause hardpan formation which restricts root growth, thus reducing plant productivity. But cooperative AAES- U.S. Department of Agri- culture-Agricultural Research Service (USDA-ARS) research indicates that using an in-row subsoiler in a conservation tillage practice can help avert soil compaction im- mediately beneath the row. An experiment was conducted at the E.V. Smith Research Center, Shorter, to determine the effects of tillage and traffic treatments on physical condition of soil. For the study, intensive soil sampling was conducted at the conclusion of a five year wheat-cotton double cropping system. A special research vehicle, the USDA- ARS Wide Frame Tractive Vehicle (WFTV), was used as a platform to conduct all opera- tions. This vehicle allows for a 20-foot cropping zone (eight 30-inch rows) that can be kept free of traffic by farm equipment. A tractor was driven on plots designated for traffic to simulate equipment traffic from normal farming operations on both wheat and cotton. Several different tillage treatments for cotton were used to determine the interac- tion of traffic with tillage systems. These treatments included: (1) CT-SS = conventional tillage with no subsoiling (disk, field cultivate, and plant); (2) CT+CD = conventional tillage with ini- tial complete hardpan disruption (disk, field cultivate, and plant); (3) CT+SS = conventional tillage with in- row subsoiling (disk, field cultivate, in-row subsoil, and plant), and; (4) NT+SS = no-tillage with in-row subsoiling (in-row subsoil and plant). Each tillage treatment had both traffic and no-traffic treatments. Soil penetrometer readings were used to determine the depth to the hardpan. This measurement is important because it indi- cates the depth of effective crop rooting. The soil condition resulting from years of continuous tillage and traction research provided vital information 4 about the damaging effects of traffic on soil that was conventionally farmed. In the conventional farming system without any deep . tillage (CT-SS), traffic ~ caused the depth to the hard- 20 pan to decrease by almost 22% and thecottonlintyield to decrease more than 14% 28 (see table). Untraf In the tillage treatment middl that was completely dis- Cone index co rupted with a V-frame traffic is appli subsoiler at the beginning of the five-year study (CT+CD) traffic caused the depth to the hardpan to decrease by 35% and cotton lint yield to decrease by 14%. The benefits of complete disruption disappeared and this was the poorest yielding tillage treat- ment when traffic was not controlled. When an in-row subsoiler was used with conventional tillage (CT+SS), traffic did not affect the depth to the hardpan, but de- creased cotton lint yield by almost 9%. With the NT+SS treatment, traffic actu- ally had a positive effect providing a cotton yield increase of 2%. These plots were the most productive and had maximum yields of any in this experiment. Traffic nega- tively affected the depth to the hardpan slightly, but this was less than 5%. One reason that traffic was not detrimental in DEPTH TO HARDPAN AND 1991 CorroN LINT YIELD AS AFFECTED BY TRAFFIC AND TILLAGE TREATMENTS Depth to Cotton Treatments hardpan lint yield In. Lb./a. No Traffic CT-SS .............................. 9.1 955 CT+CD..............11.0 912 CT+SS ............................. 14.6 872 NT+SS............................. 16.9 957 Traffic CT-SS ........................ .... 7.1 815 CT+CD ........................... 7.1 786 CT+SS .............. 14.6 794 NT+SS..................... 16.1 978 ntours for the NT + SS tillage treatment when ed to row middles. the NT+SS treatment was that the soil structure was able to support the applied traffic loads and withstand the damaging effects of traffic. This is illustrated in the contour plot that shows a profile of the crop row and traffic path for tillage treatment NT+SS subjected to traffic (see figure). Even though traffic did compact the soil beneath the row middle, the in-row subsoiler provided adequate rooting depth beneath the row. This experiment indicated that in con- ventional tillage systems that did not in- clude an in-row subsoiler, traffic negatively affected both the depth to the hardpan and crop yields. However, when the conserva- tion tillage practice of in-row subsoiling was used, the resulting soil structure al- lowed the soil to withstand the detrimental effects of traffic. Though farmers don't have WFTVs, they can control the negative effects of traffic and maintain surface resi- due cover by combining conservation till- age with an in-row subsoiler. Raper is Adjunct Assistant Professor of Ag- ricultural Engineering and Agricultural Engi- neer with the USDA-ARS-National Soil Dy- namics Laboratory (NSDL), Reeves is Adjunct Associate Professor of Agronomy and Soils and Research Agronomist with USDA-ARS-NSDL, Burt is Adjunct Professor of Agricultural Engi- neering and Research Leader with USDA-ARS- NSDL, and Torbert is Soil Scientist, USDA- ARS, Temple, Tex. Alabama Agricultural Experiment Station 3 J.H. WILHOIT, L.J. KUTZ, D.E. FLY, AND D.B. SOUTH J It A _-v OW COST ore than 1.5 billion pine seed- ling.s ar e planted in the South e/ Lvxry year. The success of for- est regeneration activities is strongly influenced by seed- ling quality. Better quality seedlings tend to have higher survival and groowth rates. AAES research may soon provide a fast. economical way to assess seedling quality. Quality is usually assessed by exaluating nmorphological properties. sueh as root col- lar diameter and shoot height. However. typical Iorest nurseries bundle and ship up to sexeral hundred thousand seedlings per day, making quality assessment of' e'ery seedling impractical. Nurseries therefore se sampling techniques to monitor seed- ling quality. but the measurement of large numbers ol seedlings is still required. Re- seairch on seedling quality improxement also involves the measuirement of large numbers of seedlings. Measuiing seedlings by hand is slo and subject to ineasurement and recoirdine er- rors. As ailahle electronic measurement methods are either destructixe or nearly as slowx as manual measurements because ex tensive operator input is reqLired for every seedling measured. AAS investigators haxe developed a low-cost machine vision systemi to quickly measure and record pine seedling plroperties. The PC-hased system. xxhich uses Charge-Coupled-Device (CCD) video cam- eras and digital image processing tech- niques. ielies on backlighting and manual seelling placement in a specified location to simplify image processing requirements. An i mportantl feature of the system is the use of multiple cameras to maximize reso- lutions li different seedling parts. The systemi measuires root collar diameter, shoot height. and shoot and root projected areas. Seedling measurements are acquiired and automatically recorded with a single flip of a switch. Tests were condicted with both two- carne ra and three-camera eon Iifieurations of the system. The two-camera conf icuration had one camera focused on the iroot coll ar area. while the othercamera had a field 1)1 iexx encompass- in- the entire seedline. The three-camera con fi f ration had one camera fo cused on the root collai area, one oi the shoot area. and one oin the iroot area. This configuration ixie" higher image resolution, for the shoot and root pio- jected area measurements Tests exvaluated how well machine vision measure- ments of root collar diam eter and height compared PC-based mach to man ual measurements and hoxw welI machine xision protected area measurements compareul to dry xeights and to projected area measurements ob- tained dlestructively with an infrared line- scan dex ice. For the manual comparison tests, each seedling xas measured by two persons so that the x ariability inherent in manual inea- surements couild be assessed. Root collar diameter and height results showed good agreement between machine vision and manual measurements. Variability fhr ma- chine xision measurements compared to manual measurements was no creater than variability for manual measurements com- pared to each other. Projected area measurements, which can- not be measured by hand, are of interest because of their potential for predicting xeight. surface area, and xolume. Machine xision shoot and root projected area mea- surements xere moderately conrelated to dry weicht measurements. The line scan de- vice, or area meter, was used as an indepen- dent standard to asscss the accuracy of the machine xi sion projected area measurements. Machine x ision and area meter projected area measurements were highly correlated. The two-camera system had a much shorter cxcle time than the three-camera ilne vision system for measuring pine seedlings. sxystei (about six seconds per seedling xer- sus IX seconds per seedling). but both of these cycle times can be reduced suibstan tially by upgrading the micropiocessor used in this study. A fast cycle time is desirable for makinc measurements on a large num- her of seedlings, but manual seedling place- ient xill require at least a few seconds for each seedling, so there is a limit to how rapid the cycle time can be. The higher resolution of the three-can era configuiration did not imprxove the accu- racy (f projected area measurements as expected although it is difficult to make a direct comparison because separate tests were conducted with each confiur ation. Foi seedling measurements in at nursery pro- duction setting. the two-camera configura- tion may be better because of its simplicity and speed. For research purposes. there may be additional measurement requirements. especially related to roots, that can be met xith a three-camera configuration. This study demonstrated the feasibility of rapidly measuring pine seedling morpho- logical pioperties using low-cost machine vision technology. Wilhoit and Kutz are Assistant Pirofessors and Fly is former Research Graduate Assistant of Agricultural Engineering: South is Associate Pro- lessor of Forestry. Aluabani Agricultural E pc r-itnce o Station i ~. Y i. ~ LILI. L11*ib~j~ ~3LXSLi,,~ 12 r,.~-, rr,, TTrCI:~, (5,\ c:rr24 J.C. BLISS PUBLIC OPINIONS PRIVATE FORESTS erccie cld eirionmental etfects are the Iitmnus test for public approx al of forestry practices. according to an AAES study funded paltl h the Alabama Unix ersities/T enncs see Valley Authority Research Consortiurn. A statewide telephone surey of 1.)07 randchmly, se I eeted Iou sehold s indicated th at Alabamians those who own forests and those wh o do not care about enx ironimein- al coiiservatioin. P'erhaps the nost hotly debated issue in forest management is the tradeoff between envi ronimental proitection andi private prop- crtyx riehts. The study indicated that most Alabamians want a halance of these values. but one that faxvors the envx iron ment. [Iully 841r agreed with the statement. "Prixvate property rights are important but only if they don't hurt the enironment." A majority supported government regulations to proteet natural beauty. streams and wet- lands, and threatened and endangered spe- cies. Most of the forest owners interv iewed su ppo irted regulations to poteet wetlands and endangered species. However. strong majorities also supported compensation for Iorest owners xhen regulations cause them to lose money. [he survey further indicated that much of the public accepts cutting trees as a neees- sary forest manage ment praetiee. Sexenty- two percent agreed that "Trees are like any othei crop. and they should he eut and re- planted to provide consumer products.'' Morover. 55( felt that the amount of tree cuttincg in their area is 'about right." This apparenit acceptance is conditional. hoxx ex er. upon the lack of adxverse enx iron- mental effects. A central concern for the environment appears to drive opinions about timber harrvesting and other forest iaiage ment practices. (learcIutt ing remains at contested prac- tice. with slightly over half the pub!ic ap- proying its use on private lands. Similarly, half approxved of the use of prescribed I ire to cont rol comipeting vegetation on prixate land. In contrast, chemical weed control. although loh, used in aearieultuie, has h ct to xin xidespread public acceptance for forest man agemcnt. Only 33r agreed ' xxith the practice. 4 G Those who oppose clear cutting. prescribed burnin. and chemical xeed control base theii Fully opposition on the per- 84/ ception that the prac a tices destroy ildlife. agreed deplete dwindling re- With the sources, or hax e othei negative enxironiien tal consequences. "Private Unf amiliaritx wih proper forests and the cm iron- mental effects of man- rights agement actixities are likel!' caused at least i r soliC of this disap i proval. For example. but although Alabamai for only est acreage incieased dramatically oxer the if they past 5) years. 77(/( be- don't liexe forest land de- creased ox er that time. hur Asked how often the landowners spray pine environmi forests with chemicals. 61( said thex did not know. and 26(/ think spraying is done at least yearly. In tact, the most intensixely managed piiie plantations in the South are sprayed at most three times dui ng their entire Ifi etiime of about 35 years. Although most respondents were not xelI informed about forestry, they were fairly educated: 85( completed high school. and half had some college training. Respon dents were from all walks cf life, including- professionals, managers entrepreneurs and others. Opinions xw ere based not onlx on at limited knowlledge of forestry bct also oi personal observations, expertise in other areas. and news coxerage of enxironmental issues. Forestry professionals may find in these results a xaluable lesson: forestry actixities perceix ed to protect or enhance the ennviron- ment are likely to enjoy public approval. Both the piblic and forest oxners are likely to disapprove of practices that do not match this perception. The profession faces dlal challenges: to continue to develop envi- ronmentally frienlly managemcnt meth- ods and to educate a skeptical public. Bliss ks Assistantt Profoessor- of For esnrv. Albaniam At,'ric ltiura/ Exerei-nent Station S.S. GROPPER AND H. C. CHAUNG IMMUNE STATUS OF CHILDREN WITH PHENYLKETONURIA Infants born in hospitals throughout Alabama and the U.S. have their heels pricked about 72 hours after birth to obtain a blood sample that is tested for the presence of an inher- ited disorder called phenylketonuria or PKU. PKU is a lifetime condition in which the ability of the body to breakdown pheny- lalanine, an amino acid found in protein, is impaired. The incidence of PKU in the United States is about one in 12,000. If not treated, PKU results in severe men- tal retardation, but if diagnosed and treated with diet therapy, there are no harmful ef- fects. Infants diagnosed with PKU are put on a diet that restricts phenylalanine intake. Infants and children with PKU must avoid high-protein foods such as milk, eggs, cheese, yogurt, meat, fish, and poultry, and must limit their intakes of other foods con- taining protein such as breads, grains, pas- tas, and vegetables. Thus, simple food com- binations such as cereal with milk, or a sandwich with peanut butter, cheese, or lunch meats cannot be consumed. Specially designed formulas referred to as medical foods provide infants and chil- dren with the majority of their daily needs for protein, vitamins, and minerals. Diets of children with PKU are very restrictive in the choices of foods allowed, and often as the children get older compliance with the diet diminishes, resulting in higher plasma phenylalanine concentrations. Adherence to the diet, however, can also lead to im- paired mineral status and alterations in plasma amino acid concentrations. Deficiencies of amino acids such as phe- nylalanine and tyrosine, minerals such as iron, selenium, and zinc, and other nutrients diminish immune system function. For ex- ample, nutrient deficiencies can result in decreased production or function of: (1) an- tibodies that help destroy foreign substances in the body; (2) complement, a group of proteins that work with antibodies to de- stroy foreign substances in the body; (3) interleukins that influence blood cell func- tion and ultimately antibody production; and (4) white blood cells that can directly kill or assist in the destruction of foreign substances. Children with PKU may be at risk for impaired immune system function because of the PKU condition itself or be- cause of the diet therapy used to treat PKU. Investigators at the AAES have begun to assess selected aspects of immune system functioning in children with PKU on diet therapy. Twenty-two children with PKU were re- 14 12 10 _J 8 6 4 2 0 Concentration of three major antibodies (IgG, IgA, IgM) in children with and without PKU. cruited from four centers that treat PKU in the United States. Blood samples were ob- tained from each child to examine cellular and humoral immune system function. Re- searchers measured white blood cell num- bers and concentrations to examine cellular immunity, as well as concentrations of anti- bodies, complement, and other proteins in- volved in humoral immune function. Immunological data of PKU children who were maintaining normal plasma phe- nylalanine concentrations were compared with those children with PKU who were not adhering to diet and thus had relatively high plasma phenylalanine concentrations. Data from children with PKU were compared with published data from age-matched chil- dren without PKU. While no differences in immune status were observed between chil- dren maintaining normal versus high plasma phenylalanine concentrations, as a group children with PKU had lower con- centrations of certain antibodies than chil- dren without PKU (see figure). No significant differences in plasma concentrations of other antibodies, comple- ment, and white blood cells were found be- tween children with and without PKU nor within children with PKU with differing plasma phenylalanine concentrations. No correlations were found between plasma phenylalanine concentrations and immuno- logical parameters. Results of this research suggest that mod- erate differences in plasma phenylalanine concentrations do not appear to affect se- lected immune system parameters. This does not mean that it is safe to exceed the recommended maintenance of plasma phe- nylalanine concentrations, since plasma phenylalanine concentrations in excess of normal ranges can lead to declines in arith- metic, language, and perceptual skills; shortened attention span; impaired short- term memory; and visual motor perception and motor coordination problems. Further studies are needed to investigate causes for lower than normal concentrations of anti- bodies in children with PKU, as well as causes for impaired nutrient status of chil- dren with PKU on diet therapy. Gropper is Assistant Professor and Chaung is former Graduate Research Assistant of Nutrition and Food Science (now Associate Professor of Veterinary Medicine, National Ping-Tung Institute of Agriculture, Taiwan). Alabama Agricultural Experiment Station IgG IgA IgM L.M. SMITH, A.G. APPEL, T.P. MACK, G.J. KEEVER, AND E.P. BENSON INTEGRATED PEST MANAGEMENT EFFECTIVELY CONTROLS SMOKYBROWN COCKROACHES common insect around homes in Alabama. Control of this insect takes many forms, including the use of insecticidal sprays by homeowners and professionals. Indoor in- secticidal sprays used by homeowners do not effectively reduce abundance of cock- roaches, because cockroaches residing out- doors will reinvade when the chemicals dissipate. AAES research explored options for out- door control of the smokybrown cock- roach. These studies focused on improving management strategies, based on the insect's behavior, that provide effective control yet reduce insecticide use. Two outdoor trials were conducted to evaluate the efficacy of two different man- agement strategies in 18 homes in Auburn during the summer of 1992. Three experi- mental treatments were used: a no-manage- ment control, a perimeter spray, and an intergrated pest management (IPM) ap- proach. The perimeter treatment consisted of spraying insecticide three feet up from the base of the house and 10 feet out into the yard with a power sprayer. The spray was a wettable-powder formulation of chlorpy- rifos at the rate of five ounces of active Reduction in cockroach abundance, pct. -60 ' TRIAL ONE -40 -20 0 20 40 T;0 00 60 80 100 10 0 10 20 30 40 ingredient per 100 gallons of water. This is one approach used by professional pest con- trol operators in Alabama. The IPM treatment combined two tac- tics: applying an insecticidal spray to crev- ices around the outside of homes and re- moving litter and debris from within a foot of the house to remove cockroach habitats. Crushed gravel was used near the homes to replace the debris. After application of treatments, cockroaches were sampled weekly at each house to assess changes in their abundance. Cockroach abundance was reduced ap- proximately 40% in the first trial with no difference noted between the IPM and pe- rimeter treatments (see figure). However the IPM treatment was safer and less expensive because it reduced chemical usage by 75%. In the second trial, the rate of chlorpyri- fos in the perimeter treatment was increased to the mid-range of recommended rates, eight ounces per 100 gallons of water. Three additional tactics were included in the IPM treatment. The first additional tactic was an application of a pelletized ant-and-cock- roach bait containing 0.5% chlorpyrifos, which was applied within three feet of homes with pine straw, fallen leaves, or ivy, and next to other cockroach habitats, such as garden borders, large rocks, or railroad 50 -10 0 10 Days after application Reductions in cockroach numbers for each trial. ties. The second tactic added was the appli- cation of a gel bait containing 2% hy- dramethylnon, which was applied in shel- tered crevices, such as in porch corners, under ledges, in crawl space gratings, and under garage doors. The third tactic was the release of insecticidal smoke in out- buildings and crawl spaces. The smoke contained 12.5% permethrin. More than 80% reduction in cockroach abundance was achieved in the second trial, nearly twice as much as in the first trial. In addition, the IPM treatment reduced abun- dance longer than the perimeter treatment (see figure). The IPM treatment maintained cockroach populations below those in the perimeter treatment for the duration of the trial, using only one-fourth of the insecticide of the perimeter treatment. This second IPM approach proved ef- fective because it took advantage of several aspects of cockroach behavior. Insecticidal sprays and the insecticidal smoke kill cock- roaches where they hide during the day. Pelletized and gel baits attract hungry cock- roaches to feed on insecticide in their nor- mal feeding areas, close to where they hide. Finally, removal of litter reduces hiding places next to the base of homes and reduces cockroach activity near doors and other en- trances. By targeting management tactics at cockroaches where they hide and forage, use of in- secticide can be decreased and cockroach control can .atment eter be maintained for a longer period than by employing a standard perimeter spray. Thus, the IPM treatment ap- pears tobe an effective, safe, andeconomicalwayto man- age outdoor cockroaches. Smith is Post Doctoral Fel- 30 40 low, Appel and Mack are Asso- ciate Professors, and Benson is Assistant Professor of Ento- mology; Keever is Associate Professor of Horticulture. Alabama Agricultural Experiment Station 7 T. P. MACK j1AICN ULAR INSCTICIDES HARMLI. ARTHROPODS IN PEANUTS L csser cornstalk horers can reduce peanut yield and quality. espe- cially in hot, dry weather, when potpulation outbreaks are likelx to occurf. Recent AAES research in- dicates that insecticides applied too early may lose etectiveness before outbreaks oc- cur, and may have a double negative impact by killing oll beneficial insects that prey on lesser cornstalk borers. In AAES research, grarnular insecticides were especially damaging to beneficial arthropods such as striped carw igs anid fire ants that livx on the soil sulrace. wheie the granules are applied. It is important to maintain an abundance of beneficial arthropods in peanut fields because they pro- x ide cheap and effective control of many insect pests, including lesser cornstalk bor- ers. Destroying beneficials also increases the abundance of some insect pests in (ther crops. Field experiments were done at the Wiregrass Substation. Headland. Sun-run- ner peanut seeds were conventionally planted in a I)othan sandy loam soil, usingi a randomized comiplete block design in both years. with four replicates of fix e treatments and a nontreated control. Plot size was eight rows wxide by 5) teet long. Herbicides and fungicides were applied according to Alabamna ('ooperative Extension Service recommendat ions. Insecticides used were Lorsban I J 1 at two pounds of active ingredient (a.i.) per acre. MocapR 15G at three pounds of a.i. per acre. [\IifonaCR I OG at two pounds ot a.i. pet acre. Fortress? 1OG at one-hall pound of a.i. per acre. CounterB 15G at two pounds of a.i. per acre, and a nontreated control. Treatments were applied at peanut flowering with a small-plot granular appli- cator. Pittall traps were used to sample for arthropouds that liv e on the soil surface. Pitfall traps were used be- cause granular insecticides remain on the soil suiface. so their greatest effects should be on arthropods that live and craw I on the surface of the soil. Each trap was conposed of two plastic drinking cups that were buried to their tops in the soil, filled one-third full with antifreeze, and covered xx ith a 12-inch aluminum pie plate held above the ground with nails. The pie plate was hung over the traps to deflect water. Traps vere monitored weekly for at least sexen weeks after applica- tion. Contents ot traps xere remoxed re- turned to the laboratory, aid identified. Applications of granular insecticides had very transitory effects on insect pests such as the corn earxxorm and fall arin xorm. The applications had no effect on beneficial arthropods such as bigeyed bugs and spiders that inhabit the foliage (Table I). Predators such as striped earwigs and red imported fire ants were the most abundant beneficial insects. Abundance of these predators declined in sonie treated plots tor Pesticide programs designed to kill harmful insects, such as this adult male lesser cornstalk borer, can also kill beneficial insects. as long as one nionth. which would increase the probability of economic darage from insect pests such as the lesser cornstalk borer (Table 2). A potentially serious result of applying a granular itsecticide to peanuts is the re- duced abundance of fire ants and striped earwigs. These two predators were the most abundant arthropods sampled. so the nega- tix e effects of oiranular iisecticides could increase the probability ot economic damage from lesser cornstalk borers and other insect pests. These results underscore the need for timely applicatfons ot insecticides based on scouting. Mack is Associate Professor ot Entonology. TABLE 1. ABUNDANCE OF SELEC1Ett PREDATORS UY TREATMENT COMBINED OVER Ai L S \ti't i DATES. I 990 Insecticide Imported Bigeyed Spider Spider Ground fire ants' hugs (calopy)' (soil-dwelling)' beetles' Nontreated 2.......... 20.8 0.8 0.2 1.7 3.9 Iorsban .............. 4.2 .6 .2 1.2 4.3 Mocap .......... 7.3 1.3 .1 1.3 4.4 DU tinate ............ 9.6 .0 .3 1.7 4.5 Fortess ................ 8.8 1.1 .3 1.7 52 Counter .......... 2 .6 .4 .0 4.5 Number per 0 rap per week from pitfall traps. 'Numher per six row-feet per week, Irom beat sheets. TABLE 2. ABUNDAsNCE OF STRIPED EARNN IGS I OR 35 DAYS At til TRFL SItEN I oN Jit 5, 1990 Days atter treatnment Insecticide 5 12 19 28 35 Nontreated ........... 14.9 8.6 23.) 112.5 100.5 ILorsban ............... 3.9 4.1 13.1 89.4 10)26 Miocap ............... 29 5.3 2(.3 90) 135.1 D) fo ate .............. 5.4 6.6 25.5 97.9 133.1 IFortiess .............. 1.5 69 36.8 1(119 132.1 Counter ................ .6 2.1 10.8 89.1) 130.9 A lubmni rAu'icultiru/ Lvjwtimcllewl Station I i . I2r D.G. HIMELRICK, W.A. DOZIER, JR. A.W. CAYLOR. J.A. PITTS. M.H. HOLLINGSWORTH, AND W.B. WEBSTER MAI TED RZow STRAWBERRY VARIETY PERFORMANCE IN CENTRAL AND NORTH ALABAMA eletring thre best perlti iiru S .ri ctx Sis crucial to the success (If a cor- mercial strawberry operation. Al- th ughi miany xvarietires are axvailable, only a fewx will povide high yields arid superior fri t quality. WhleI the econoImics ot Successful pro- duetiori make high yields a primary conisid- crationi x hen selecting x aricties. additional fruit quality characteristics rmust be conisid- erecd in the final decision. Other citerra in- elude cornsisteriex of annrural cropping. x igor. runner-riak inrg ability. disease resistance. and othle r pl ant penforrmance tactors: as wAelI aS her- ixse, firmiiness sugear content. tiax or. and othe icrhiuit quLial ity characteri stics. Varieties arec categorized as early-, mid- or late-season in ripening. Ditffer ences iii lie fii st hiarx est date usuiallIy do no( t xvar-y great ly amronrg xvariet ies. The gr-eate st dillfer- ence iii ripeing is the tirie (If peak hiarx est. wh lich may x ary sexven to 10) days betweecci the earliest andI latest ripening x arieties. Ini somiie xvears the dif fererice ini the ipeinrg seasonis may be short and peak harx est sea- soils oIt the xvarieties ovxerlap. Straxx brrx xvariety trials we rc condiulctedl at the (Chrltori A-rea Horticultuire Substation (CAHI S) ini 1horsbx. Nor th Alabama Hor- tICUItUirC Suhsationl (NAHS) ini Cullmian. and~ ITenniessee Valley Substation (TVS) iii Belle Miria. In the f irst e-xperirient, 21 xvarietics were plantedl at the NAHS and TVS. lIn a seconrd. 16 x arieties 'a erec planted at the CAHS arid TVS. Ini a third test. 23 xvarieties xwere planted at the CAHS. The x arieties cx aluated xxere Earlibelle. Sunrrise. Far liglow. Titan. Preludue. Surecrop. Pocahontas, Sco(tt, Cardinal. Ark6086 . Allstar. Atlas. Douglas. Parajo. Vista. Rosanine. Sentinel. Ar k6686. A-rkinig. Delite. Tennessee Beautx. Redchiet. Ti(Iea. Albri tto n, A pollo. C hand ler. A iko. Guard- iam. I lonce, C Lester. Sequoia. LateeLdx \m Mllate. I ar-e seasonal and location fluctuationis \\ e I seen in miiany important performuance loir acteristieS, Such as yield and berry sirc. ]'be seveni ox erallI best v arijetiCs we re larligloxk. Sunrise. Titan. Allstar. Cardinal. Scott. and Dcllite (see table). Scx eral top-yielding x arieties wxerc not in the top sC\ en duc to additronal undesir- able traits. F-or example. Tennessee B~eaurty has a relatixvely soft, small berry and ave r- aiee dessert arid processing quality . Apollo prodirces Sterile pollen and variable x relds. as we ll as being droug~ht Susceptible. Alter- natrxely, somrie ax er age-xieldriie xarictics. such as [-arligloxx are listed because of other char acteristrcs, such as earliness and exceptiorially good flavor and processing quality for freezinrg. Moire detailed inflormation conrcerinrg the 3 xvarireties tested in this Study is pre- sented ini \AA S Bulletin 619," Pertotrunce (it Sti- ax brrx Cultix ars in Central arid North Alabama Growxn on the Matted Roxx Sy stem.. Professor - and (,i\ or is Research Specilist of HoutICUliure: Ptti. Hoinesxiirth, and W\ehster are Superintendents of the Chitton Area Hoirti- culture. North .\lahana Horticulture, and Tennes- see Valley substations. irespects ely. OP' tI iiRMiI STRAW\ iii RRSv V~ Sll hiSs i Ri< i OS F SI \i \ Yi SRS 550U TuREE LOt ArIONS Vai lit t-arli'tii .............. t au i . . . .. . . . .. . . .. . . . Sunse ............ ... A llt at ............... Carn n . ............. S co t . .. . . .. . . .. . . .. . . . D elit .. . . . .. . . . . .. . . . . Torat Harvst Ai verage tPerceini heirs s ietd seaisin fruit soiluile liiniiiess' w~eight solids' Lb./a 7,170 8.761 8&439 8.806 8.3(02 9.582 10.040 F-.rN Early E-ail s Mid MIid Mid Lt ae Gr,,ot 6.9 9.8 9(0 1012 10.2 92 10.0 Degrec exterior colo~r 4.5 4.2 4.2 3.6 4.5 4.4 3.9 Degree itrio( clorl 4.2 3.6 3.7 2.5 4.1 4.1 2.7 Ltisteid iii order of ripening. 'One oun tie eqa it29 grams. 'The hither rthe peicent of SOluble solids. the sw~eeter ihe berry. I irnniess dutne: I = ver s~oft. 10) = ery firm. tDeuiee of color: I =green. 5 = dark red. Al/u/bm,, Agricirutua F_.r1eijnew .Statioum T.A. MCCASKEY, S.D. ZHOU, S.N. BRITT, AND R.C. STRICKLAND CAN SOLID WASTE BECOME A RESOURCE INSTEAD OF A LIABILITY? The U.S. discards approximately 200 million tons of municipal solid waste (MSW) each year, creating many disposal problems. Auburn University and Tennessee Valley Authority (TVA) researchers have demon- strated a promising method of recycling MSW into a valuable raw material that can be used to produce biodegradable plastic. Landfills and incinerators are now the primary means of MSW disposal, but they may cause water and air pollution. And while MSW production is increasing, the capacity to handle it is decreasing. M00Many landfills and incinerators lodegradable have been closed, and sites for new ki!istic may disposal facilities eplae are often difficult to locate. Recy- .... cling is one of the he 1 5. . . most promising . ways to manage ilon. . .MSW and reduce f.S its environmental Simpact. DMVO lORMI !i Newsprin t, Iastik wood, cardboard, and yard waste - s d in which comprise he U.S.. 56% of all MSW - are primarily very year. made of plant fi- bers called cellu- lose, a network of thousands of simple sugar units. These wastes can be broken down into individual sugar units, chiefly glucose and mannose, using sulfuric acid. The sugars can be fer- mented by microorganisms to produce a variety of fuels and chemicals. Microbial fermentation has been used for many years to produce ethanol from the glucose found in corn. Recently, a 100% biodegradable plastic was manufactured from lactic acid and ap- TABLE 1. E proved for use by the Food and Drug Administration. This Bacteria evaluate( product may replace some of the 15 million tons of conven- tional plastic used in the U.S. L. arabinos every year. This development L. arabinos has stimulated interest in pro- L. arabinos ducing large quantities of in- L. pentos expensive lactic acid. MSW L. pentosus could provide a low-cost L. plantaru L. xylosus 4 source of sugars for lactic acid production, in addition to re- cMSW ducing the volume of waste in 2 One p landfills. milliliters. AU and TVA researchers TABLE 2. O are developing the technology to use microorganisms to con- vert MSW to lactic acid. The Fetiment waste used in these experi- ments was newsprint that had Days been treated with 2% sulfuric 0..... acid at 250?F causing the pa- 1................. per to break down (hydrolyze) 2....... 3 ................. into its component sugars, 4....... yielding 4.1% sugar. 5................. Since the hydrolyzate may 'Total su contain compounds harmful was added. to lactic acid-producing bacte- One pou ria, it is treated with calcium hydroxide and filtered. Nitrogen supple- ments, which the bacteria require for growth, also are added. An evaluation of eight types of bacteria showed that one in particular had the poten- tial to produce high levels of lactic acid from MSW (Table 1). Culture L. pentosus 227 produced 0.187 pound of lactic acid from one gallon ofhydrolyzate (2.24 grams per 100 milliliters). To further improve the ability of the cul- ture to produce lactic acid, the sugar con- centration of the MSW hydrolyzate was increased to 8.2% and the hydrolyzate was buffered by adding 5% calcium carbonate. Since lactic acid is produced from sugar, the increased sugar should improve lactic acid production. The higher buffer capacity should help alleviate inhibition of lactic VALUATION OF LACTOBACILLUS CULTURES FOR PRODUCTION OF LACTIC ACID FROM MSW 1 SSugar used Lactic acid Sugar to d produced lactic acid conversion g/100 mL 2 g/100 mL 2 Pct. us 787 ........ 3.04 1.86 61 us 788 ......... 3.01 1.82 60 us 813 ......... 3.33 1.70 51 us 531........ 3.18 1.67 52 227 ............ 3.63 2.24 62 473............. 3.84 1.93 50 m 422 ......... 2.95 1.96 66 449 ............ 1.83 0.82 45 hydrolyzate contained 4.1% total sugars and no added rbonate. ound equals 454 grams; one gallon equals 3,785 PTIMIZATION OF LACTIC ACID PRODUCTION CONDITIONS FOR CULTURE L. PENTOSUS 2271 ation Sugar Lactic acid Sugar to conversion g/100 mL 2 g/100 mL 2 Pct. ................... 0 0 0 ................... 2.00 1.54 77 ................... 6.23 5.32 85 .................... 7.49 6.50 87 ................... 7.54 6.05 80 ................... 7.54 6.04 80 gar concentration was 8.2%, and 5% calcium carbonate and equals 454 grams; one gallon equals 3,785 milliliters. acid production caused by the higher acidity associated with increased yields of acid. Under these conditions, culture 227 pro- duced 0.542 pound of lactic acid per gallon of MSW hydrolyzate (6.5 grams per 100 milliliters), almost three times more than previously demonstrated (Table 2). Industry is exploring the production of lactic acid from corn for the manufacture of biodegradable plastic. However, the use of solid waste has significant environmental advantages. Studies are in progress to further improve the process of converting MSW to lactic acid. McCaskey is Professor, Zhou is Visiting Scholar, and Britt is Research Associate of Ani- mal and Dairy Sciences; Strickland is Project Manager, Biotechnical Research Department, Tennessee Valley Authority. Alabama Agricultural Experiment Station 10 J.L.KOON, C.A.FLOOD,JR., R.D.TRUMBULL, AND R.N.BREWER ENERGY SAVINGS WITH USE OF DOUBLE SIDEWALLCURTAINS ON BROILER HOUSES Heat energy is used by broiler producers during the first three weeks of a growout to provide an optimum environment for bird survival. Depending upon time of year, brooding energy used during this time will range from 75% to 100% of the total energy used for the entire growout period. Heat supplied during the last four weeks serves primarily to improve feed conversion and efficiency. Since LP gas, which is the primary source of heat utilized in broiler houses, is expensive relative to other energy sources, conservation is a major concern of broiler producers. From an energy use consideration, the ideal poultry house would be a totally en- closed, insulated house for the brooding period with open curtains during the re- mainder of the growout. For curtain sidewall houses that are cur- rently being built in Alabama, the portion of the house energy losses that can be attributed to curtain area on houses using single cur- tains and half-house partial-area brooding is approximately 66% of the total for the house. The use of double curtains reduces this por- tion of the total house energy loss due to the curtain area to 48%. This has the potential to reduce the overall heat loss from the house during the first three weeks by some 35%. A study of this concept was conducted by the AAES during four 48-day cold weather trials at the AU Poultry Research Unit to show the overall effect on energy use when using double curtains as compared to single curtains on poultry houses. While every effort was made to operate the sys- tems in a manner similar to that used in industry, the size and type of house used in GAS USAGE FOR THE FOUR TRIALS, GAL. LP Trial Single Double Pct. reduction curtain curtain Trial 1 .......... 466 357 23 Trial 2 .......... 208 187 10 Trial3 . 238 180 25 Trial 4....204 156 24 the demonstration prohibited the utilization of partial house brooding. Because partial house brooding was not used, total energy consumption was higher than would be ex- pected in a typical commercial house. Comparisons of the relative amounts of energy used and relative energy savings can be made, however. Two houses were equipped with single curtains, and two houses were equipped with double layers of curtain material. The curtain material for the house containing double curtains was installed as shown in the figure. Air was al- lowed to enter the house by passing up- ward through the air space between the two curtains. This method of installation allows recovery of a portion of the heat loss through the curtain material and prevents conden- sation of moisture be- tween layers of curtain material, which has been a problem when double curtains with closed bottom edges have been used. One curtain was installed outside the building, and the second curtain was installed inside. The building wall was constructed using 3.5 X 3.5-inch posts, thus a 3.5-inch air space ex- isted between the cur- tains. Theoutercurtain Cross sectional v was held away from construction on the wall at the lower edge by a series of 0.5- inch tubes. During early brooding periods when ventilation requirements are lowest, air flow was through the lower opening and up between the two curtain layers. A 1.5-inch iev slot opening was established at the top of the inside curtain. Both curtains could be lowered, and ventilation air passed through a 1.5-inch opening at the top of each curtain when ventilation requirements increased. The inner curtain opening could be closed completely when needed. Both curtains can be lowered completely to provide a four-foot opening for warm weather opera- tion. The table summarizes the gas usage for the two types of curtain material for the four trials. Overall gas usage for the four trials was reduced from 1,118 gallons for single cur- tain houses to 881 gal- lons for double cur- tain houses. Use of double curtains gave an average gas usage reduction of 21%. This research in- dicates maximum benefit of double cur- tains will be obtained by installation in the wall sections of the primary brooding area and should not be required for the entire house. It also indi- cates reduction in gas usage, resulting from the use of double cur- -tains, will depend on house construction and time of year the birds are grown. The most effective use of double curtains is dur- w of double curtain ing the first three Itry house.weeks of production, and in most cases their use will be justified only in the partial house brooding area. Koon and Flood are Associate Professors and Trumbull is former Research Associate of Agri- cultural Engineering; Brewer is Head of Poultry Science. Alabama Agricultural Experiment Station 11 S.S. STRONG, C.F. DENEKE, B.K. BEHE, AND K.L. BOWEN I RAN SMISSION OF PHYTOPHTHORA ROOT RoT IN AN EBB AND FLOW SUBIRRIGCTION SYSTEM ncireasln concern a bot xxater qf al- ity has prompted the floriculture in- dustry to increase scrutiny of f ertiliz a- lion and irrigation practices to help re- duce the potential for I groundater coitaimi- nalion from green- , house runoft. Ebb and a now suirrigation is one mtethod ot control- liia ruinoillf, hut recent AAES research indi- eates plant spacing is critical in reducing the spread of disease Peppermint Cooler vinca gro withi this system. with Phytophthora parasitica. Ebb and flow sub- irrigation is a closed pioduction system These s SiVI MI N I Of PHIOPHI iIRA P0 \%AI-F( A To NON \I it rr VINCA SP.-Vl Li ATTwo Divi \N\(iSii O \im ANDi ho rNCH Spacing hetmeen pots hi. (iooip 1. 7/16 ............. Group 2. 1 5/8 ............ C wing on an ebb and flow bench; closest row inoculated pores would be discharged along with the runoff in traditional irrigation systems, but with Linulatiie intettiol of nonireated plants are contained and recircu- Week 2 Week 4 Seek 6 lated. The movement of the root rot pathogen Phi to- Pct. Pct. P. ptlihortra parasitico from dis- 6 23 60 eased to healthy plants in the 0 10 30 subirrigation system was stud- ied in an AAES project. that collects runoff and returns it to a tank foi recirculation. As the surface of a leak- proof floor or bench is slowly flooded, irri- gation water flows around and beneath pot- ted plants. Water is transported tp through the growing medium by capillary action. Altcr the sater is turned off, the irunoff is used for the next irrigation. This labor- saxing system conserves water and fertil- izer decreases croundxsater contamilation pitential, and reduces incidence of foliar tlisease. A possible dirasback is the spread of root ltscase in the iecycled liquid. Some bacteria and fungi produce spores that moxe freely in water and can cause large crop losses. Five-wxeek-old seedlings of Peppermint Cooler vinca were transplanted into three- inch pots. Six rows of plants were placed on 4 X 6f4oot ebb and flow benches. The first row placed along the drain of each bench was inoculated with the pathogen to serve as an inoculum source for the five nontreated rows. Separate benches were used to space pots at two distances - 7/16 and 1 5/8 inches. Plants from nontreated rows were barsested at txo-week interxals and root samples wxere tested for exidence of Phytophthora. Solution from irrigation tanks also wx as tested. Plants were groxxwi on the benches for six weeks. By week six, root samples from 601/ of nontreated plants spaced at 7/16 inch (Group I ) displayed infection by Phyto- phthora. while 30'4 of those spaced at 1 5/8 inches (Group 2) ex- hibited pathogen growth. Diseasekilled all of the inoculated plants. and Phyto- phthora spores were detected in one of four water tanks used. The pathogen moveci from the in- oculated row to the fifth row of nonitreated plants on all benches by week six a total distance ot 17 inches in Group I and 23 inches in Group 2. However. only 1317c of infected plants in Group 2 exhibited sexere root injury, com- paied to 36% of those infected at the closer spacing. Severe injury means at least 25% of the root system is harmed. The pre-packaged Canadian sphagnum peat and perlite medium used in this study xas steam pasteurized to allows lo moire accurate detection of Phytophthoia by eliminating existing pathogens. However pastetiri/ation of Canadian peat is not rec- ommended in commercial production be- cause beneficial, disease-suppiessingl mi- croorganisms also are destroyed. Sanitation of production areas and disin- fection of equipment reduce chances of1 a disease outbreak. but other measures may become necessary once a pathogen is estab- lished in an ebb and flow system. Chemical pesticides are commonly used in consven- tional production systems. however ithere are currentls no fungicides or pesticides registered for reciiculation. Stinn is Giaduate Research Assistant. )ckcec and Behe are A\ssistant Pirofessors of Horticu turie: Boms en is Assisiant Professor of Pant Patholog . A labamai A 0/rh mltiura/ E.v1 rime n .Slution J.M. DANGLER AND C.W. WOOD iN I1 (RlON hAt LS AN D V it H1N-K0iV PA(AN(NS 41- _T8- ~ a in recent year s, seed companies ha introduced new hybrid collaids tl are higher yielding than traditiot open-pollinated varieties. One hbr Blue Max. exhibited the greatest v ii stability in trials conducted in the Sothea Because ol the increased cost ol hybh seed and the conv enience ot obtaining din ity transplants. collard establishment I shifted rom direct seeding to the use transplants. -loseser. the effects ofx with row spacing and nitriogen (N) fertilizer re on collards prodnced from transplants F not been addressed. An AAES study focused on these factors in the production of Blue Max and Vates. a less expensive, open-pol- linated vaieicty. grown i a sandy loam soil. Field experiments were con ducted in the spring and tall of 1990() on a Norfolk/Oraneburg sandy loam soil at the E.V. Smith Research ('ente. Shorter. Treat- ients included N rates of 0. 50. I. 100.150. and 200 potnds per acre . supplied as anmmoniui nitrate. One-row plots were used with r4 plant spacingsof 6. J. and 12 inches. Rows were f our leet apart. Potassiim and phosphorous were broadcast anI incorporate at iates determiined by the Auburn Univeis Soil Testing Iaboratory. Half ol the N " broadcast at planting: the remainder "x band-appliet 3) tlays after planting. Wee nematotes, antI othe pests were controll accordine, to the Vegetable Pest Conti Guide ANR-2. Plants were harvestetd or each season ait the 21-leaf stage. Fall yields were 4.6 tons per acre. wh spring yielts were 2.8 tons per acre. shown in the Ifigure. Collards usually gr( better in the Iall when temperattres are co However. season, N rate, and ctiltil interacted in their effects on yieltl. In spring. Blie Max yield increased prop tionatel as N rates increased, butt 6 I- Q -64 33 a2 u. F I I I I I I I I 0 I 0 00 0 0 0 0 O O .9 D D I 150 200 0 N Rate, Ib./A Y itN highest Vates yield wxas obtaietl at 100 7as potnds of N per acre. Highest fall yields of 'as both xarieties were obtained with 100 As. potnds of N per acre. Howexer. Blte Max ed yielded more at all rates of N application. rol The superiority of Blte Max xx as espe- ice cially exident in the control plots. where N was not applied. The hybrid yielded ap- ile proximately I0(01 more than Vates in these as plots. However. it shoild be noted that Blue rw Max pirotluces bluish-green leaves that ol. some consumers may find objectionable. ai Yields of both collard xarieties decreased he from 4.0 to 3.6 tons per acre as the xx ithin- ii roxx spacing increased trom six to nine he inches. Yields tlid not decrease further at 50 100 150 200 AES study confirmed the high yield potential of Max, a hybrid collard variety. '-inch spacings. Ailts of' these experiments confirm iperior yield potential of' Blue Max. a hi id. compare to an openpol litiatet cul- mi-. Blie Max responded to the addition wure than 100 pounds of N per acre. I n the fall, the yield difference between ,_i tics when no tertilizer was applied is Jdlitional evidence of the hybrid's poten- al. Blue Max may have the capacity to )rodtce high yields when planted as a dotble crop without additional fertilizer. The similar redtctions in Nielti of both varieties xith increased xithin-row spacing suggests the crop's response to plant spac- ing is similar for hybrid and open-pollinated collards. In addition to confirming the yield po- tential of Blue Max. the results of this experiment establish a benchmark for N application at 100 pounds per acre on Ala- bama sandy loam soils. It also snggests that high yielts of standard and hybrid collards may be obtained at within-row spacings of nine inches and a tour-foot row spacing. Dangler is Assistant Protessor ot Horticul- ture and Wood is Assistant Professor of A'ionoms and Soils. A Iabainu A i- ic ultirrl E xperiloc w Stationt I L. L. LOVSHIN AND D. R. BEAM ADVERTISING INCREASES THE SALE OF LIVE CATFISH small area of water often have problems marketing their fish to a processing plant because the small quantities harvested do not warrant purchase by the processing plant. Small- scale catfish producers have to find alterna- tive markets for their fish. Two of the most attractive markets for small-scale catfish farmers are fee-fishing and live-fish sales to area residents. The Department of Fisheries and Allied Aquacultures has operated a live-fish mar- ket and fee-fishing pond located five miles north of Auburn for 13 years. The market is open on Saturday from 8 a.m. until noon about 47 weeks a year. The fee-fishing pond is open during the same time period about 32 weeks a year. Channel catfish raised in AAES research ponds are harvested in the fall and early winter and stored in holding ponds until they are sold. Enough fish must be stored to satisfy consumer demand until the follow- ing year's fish are large enough to harvest in September. Often 40,000 to 50,000 pounds of fish, 8,000 to 10,000 pounds per acre, are in holding ponds during the winter. Holding ponds that contain over 5,000 pounds of fish per acre by the end of March often have disease outbreaks that can result in high fish mortality. To reduce fish mor- tality, the weight of fish in the holding ponds is reduced to levels that permit the maintenance of a healthy aquatic environ- ment. Live channel catfish are THE INF sold for $1.20 per pound while catfish are sold to the process- Year ing plant for $0.60 to $0.70 per pound. Thus, profits are higher if excess fish in holding 1985 ......... ponds can be sold through the 1987......... live-fish market or to fee fish- 1988......... ers rather than to a processing 1989.. plant. To increase catfish sales, 1991 .... a three- to four-week advertis- 1992..... ing campaign is conducted be- Avg .... 3,: 3,1 tween January 14 and March 31. Ads are printed in the Auburn University student newspaper and two area . 2,500 newspapers at a cost of about $150 per week. The ad cam- o paign promotes a reduction in 2 the price of live catfish from $1.20 to $1.00per pound. Ads are printed on eitherThursday or Friday depending on news- 1,500 paper distribution. An average of 47,060 pounds of catfish per year and 998 pounds of catfish per 1,000 week have been sold from 1985 through 1992, as shown in the table. Advertisements in 500 local newspapers increased live catfish sales in 1990, 1991, and 1992 (see figure). The weight An average of 1,468, 2,388, during, an and 2,047 pounds per week of and 92. live catfish were sold during the periods of newspaper advertisements in 1990, 1991, and 1992, respectively. The weight of catfish sold during promotions increased 37, 50, and 47% over the weekly averages for 1990, 1991, and 1992, respectively (see table). Live catfish sold three weeks before and three weeks after the ad campaign at $1.20 per pound generated average weekly incomes of $927 and $1,216, respectively. Average weekly income received from live catfish sold for $1.00 per pound during the 1 2 3 4 5 6 7 8 9 10 Weeks of live channel catfish catfish sold before, d after advertisment campaigns in 1990, 91, ad campaigns was $1,968. Even with the $150 per week advertisement costs de- ducted from the $1,968 earned weekly dur- ing ad campaigns, average weekly income increased $891 and $602 compared with income earned before or after the promotion, respectively. Additionally, advertising appears to have a residual effect on catfish sales after the promotion is ended, as shown in the figure. An average of $289 more catfish were sold during the three weeks after the ad campaign compared to the three rHE AAES weeks before the ad campaign. Percent Newspaper advertisements increase to promote a reduction in the price of live catfish are a promising method to raise cat- - fish sales, reduce inventory in - catfish holding ponds, and in- - crease income. Lovshin is Professor and Beam is Senior Research Associate of Fisheries and Allied Aquacultures. Alabama Agricultural Experiment Station LUENCE OF ADVERTISEMENTS ON LIVE-CATFIS Sale weeks Yearly Average Av per year sales weekly sa sales Lb. Lb. ... 48 47,511 990 ... 41 46,907 1,114 ... 48 42,510 886 ... 48 46,656 972 ... 46 42,180 917 ... 50 46,280 926 ... 49 57,948 1,183 ... 48 46,489 969 ... 47 47,060 998 1,468 37 2,388 50 2,047 47 1,968 45 verageweeklwcampaig~r -1Lb. 14 J.H. EDWARDS, D.B. WEAVER, D.L. THURLOW. AND J.T. EASON ROTATING SOYBEAN CULTIVARS MAINTAINS YIELDS ticularly corn or sorghum. has been Ro a ~i oe n t a n o s ro ea cyst Irecognized as a valuable tool in the mianagement of soybean cyst nematode. Soybean cultivars that are rcsistant to soybean cyst nematode also have been Lised as a management tool. Con- tinued monoculture of resistant cultivars. however, led to the development of new races of soybean cyst nematodes that were able to reproduce on and reduce the yield of previously resistant cultivars. AAES re- search suggests that rotating resistant and susceptible varieties may provi dc a way to grow a soybcan monoculture and maintain acceptable yields. In Alabama soybean yield losses in 1992 due to soybean cyst ncmatodes, were esti- mated to be 110,000() bushels, worth $600,000. Reducing the loss by a small percentage should have a beneficial effect on net returns to the soybean producer. An AAES study was conducted at the Sand Mountain Substation. Crossville, to determine the effects of various soybean clopping sequences on soybean yield and soybean cyst nematode numbers. The rota- tion included soybean cultivars that were susceptible and resistant to cyst nematode. Cropping sequences included continuous monoculture with the same soybean cultivar using Essex (a susceptible cultivar). Forrest (resistant to soybean cyst race 3), or Bedtord (resistant to soybean cyst races 3 and 14). These plots were compared to plots in a two-year rotation of Essex-Forrest. Forrest- Essex, Essex-Bedford, Bedtord-Essex Forrest Bedf ord. Bedford Forrest. Essex- corn. Forrest-corn, or Bedford-corn. Soybean yields were lower when culti- vars were not rotated compared to cultivar rotation and rota tion with corn 50 F Continuous 20Y (see ti('ure). Yield of Essex was W Soy-Soy lower when the previous crop was Soy-Corn Bedford rather 40 than [orrest. 15 Corn in the rota- tion had the great- Z est positive ben- efit on soybean Q 30 yield. There was C 0 a oie to two - M -10 c bushel peracre in- Ui crease in yield 0 when resistant 20 cultivars were rotated with sus- ceptible ones. while there weie 5 two to four bushel per acre increases when soybeans were rotated with corn. Soybean cyst 0- 0 0 nematode larvae 1990 1991 1990 1991 and cyst counts Cyst nematodes were highest for Soybeans continious Essex Yields and nematodes counts from various treatments including con- and continuous tinuous (nematode susceptible soybeans), soy-soy (resistant and sus- Forrest soybeans.ceptible rotation) and soybean and corn rotations. Larvae and cyst counts were higher in the tode-infested soil is feasible when resistant Forrest and Bedford cultivars when follow- cultivars are rotated with susceptible ones. ing Essex. Edwards is Adjunct Associate Professor ot Based on these findings, it appears that Agronomy and Soils and Soil Scientist wxith the soybean production practices that include US)A-ARS National Soil Dynamics Lab. Weave corn in the rotation are the best means to is Professor and Thurlo is Associate Professor sustain soybean yield. However, planting Emeritus of Agronomy and Soils. Eason is Super continuous soybeans in soybean cyst nema- intendeni of the Sand Mountaii Suhstaiion. Alabama Agricultural E.xperiment Station G.R. MULLEN VVHICH HIGGERS ATTACK HUMANS IN ALABAMA? ost people haxe encountered chiggers or 'red hugs.' and experienced the intense itch ing their bites can cause. but little inform ation is axvail able regard ing the species ot chiggers n volved. )cirinv the past 15 years, researchers with the AAES coldlected in formation that helped deternmin m the species that attack hnmans and the fate of those chiggers once they haxe attache to ai unsuspecting host. (higereis are tie tiny larx al stagce of mite' ot tle fainilv Tmnbicadli. Whierteas nyiplis and adults t these mites are Iiree Iiin. the larvae are parasitic on manx vertebiate hosts iicludiii mammals. birds. reptiles, and amphibians. Chiggers seldom cause icici discomfort to their normal hosts. However, when they attach to atypical hosts such as humans, their bites often cause skin reactions, usually aiound the ankles, wrists, waist, and othei parts of the body where clothes fit snugly. Identificatiois of chiggers recovered ifro hu mans in Alabama indicate that two species are most frequently involxcl. Nei- ther has a common name. Oie is the eastern forn of, Iutrombhicu/o a/frcddui'csi. the most pesky chigger intesting people in the easterii U.S. The other is Luuotiobi u/ lipoi sk ona, which has not been repoted to bite humans. Although the lattei species was found crawling on people. researcher s are iot sure if it actually attaches aiid feeds. On the other hand, E. (Ilf-reddu'esi was clearly respoisible for most ot the discom- tortiig chigger bites experienced. A third species, E. splemle n. known to attack humans in areas bordering marshes and swamps - was not recovered, which is probably due to liiited collectiins from these particular habitats. . ulf -c(d(uigc si was found maiily in relatixely dry upland areas of piie. iixed hardwoods, and second- groxth sites domi- nated by various plants. such as blackberry and honeysuckle. In contrast. L. liposvsian was ound in low-lying deciduous xoods with damp. shaded ground cover aid an Close-up view of skin reaction to chigger bites w swelling, and vesicle formation. abundance of decaying logs and stumps. Both habitats support populations of lizards. which are preferred hosts ot these chiggers. Both species xere generally active throughout the summer and early tall. E. orifr-ceddui,'ei xas recoxered in huiman cases from early May through mid-September and I. liporskv ana froi early July through mid-October. . alftrcdducs comprised 7514 of the cases examined and 76(r of the total specimens recoxerec and ideitified. To determine what happens to chiggess after attachmenit to a peison. indiviidual mites wx ere monitored. In oie series of observations, the attachmeit sites of 25 E. afr c-ddu'esi were circled xw ith ink wi thin 1 2 limi" of ifl etatio 1 .iIh ile ie i, ALABAMA AGRICULTURAL EXPERIMENT ST/ AUBURN UNIVERSITY AUBURN UNIVERSITY, ALABAMA 36849-5403 Lowell T. Frobish, Director POSTMASTER-Address Correction Requested .naniined daily thereafter. Within Sthe 36irst 3 hours. fib;. nine had died while stiII attached at their original sitesanother nine had detalched and either dropped off or ioved to an- other, undeter- mined location. 01 the remaining seven attached ith associated redness, iites. only two xere still alive by the fourth day. None surxvix ed tol daiy five. Even the few chiggers that did manage to feed did not appear to survive oi this host. Whether the mites survived or not, their bites caused intensely itchy skin lesions, oftei culminating in fluid-filled vesicles at the point of attachmet. These ohserva- tions iidicate that few, if any, of the chig- gers that attach to humans successfuilly complete their feediig and go oi to de- x elop into nsmphs. However. unless the chiggers are killed or riemoved within a fe hours, their bites typically cause a localied. sometilies severe. skin irritatioi exven after the iites have died or detached. \TION NON-PROFIT ORG. POSTAGE & FEES PAID PERMIT NO. 9 AUBURN, ALA.