eT A 0 A A El 'urn Agrnom and: Sol eateta eisN.20 eebr19Alabam AgiutrlEprmntSainJmsE aroDrco uun nvriylbm 4;~: """ 1997 NATIONAL FUSARIUM WILT COTTON REPORT 1 Kathryn M. Glass and William S. Gazaway 2 Cotton cultivars and elite breeding lines submitted by 27 cooperators were evaluated for Fusarium wilt resistance under field conditions at the Plant Breeding Unit, E.V. Smith Research Center, Tallassee, Alabama. Entries were grown on an Independence loamy fine sand highly infested with both the Fusarium wilt fungus (Fusarium oxysporum) Schlect. f. vasinfectum [Atk.] (Snyd. & Hans.) and root-knot nematodes (Meloidogyne incognita). Plots were 40-inch-wide rows, 20 feet in length, separated by 5-foot alleys. Four replicates of entries and checks, arranged in a block design, were evaluated. Both susceptible (Rowden) and resistant (M-315) cultivars were included as checks. Rowden was planted in row 5 and every tenth row thereafter (15, 25,...,265) and M-315 in row 10 and every tenth row thereafter (20, 30,...,270) throughout the test. Plots were planted May 14. Initial plant counts were made on June 19. Wilted plants were counted and removed on July 1, July 18, August 6, August 28 and September 16. The remaining live plants were counted and recorded on September 16. Percent wilted plants were then determined and mean wilting for a given entry calculated. Average wilting of the susceptible Rowden was 80, 90, 85, and 79 percent for the four replicates (84 percent average). Corresponding wilt percentages for the resistant check, M-315, were 22, 25, 26, and 22 percent (24 percent average). Critical evaluation of a given entry should be made relative to the checks closest to the entry within each replicate. Evaluation of breeding process or evaluation of entries over years should be made only between the relative value of this entry and that of the closest susceptible check rows for each year. A soil analysis for nematodes made in 1994, revealed that southern root-knot (Meloidogyne incognita) and lance (Hoplolaimus galeatus) are two predominant nematode species in the test plots. Other nematode genera present were stubby root (Trichodorus sp.) and stunt (Tylenchorhynchus sp.). Root-knot nematodes, however, appear to be causing the major damage to cotton in the Fusarium Wilt Test as indicated by the high galling indices found on the roots of all cotton lines. Root-knot nematode damage was heavy on cotton roots of all varieties including the susceptible cultivar, Rowden, this past growing season. Most cotton varieties and cotton lines had a very high root-knot galling indices this year. Entries submitted by Kathryn Glass are commonly grown cultivars or advanced commercial materials and are listed by name. Entries submitted by other cooperators are listed by their coded numbers. Additional information regarding the genetic background of a specific coded entry should be obtained from the named cooperator. 1 This report is a joint contribution between USDA-ARS, Crop Science Research Laboratory, Mississippi State, Mississippi, and the Alabama Agricultural Experiment Station, Auburn University, Alabama. 2 Glass is a Research Assistant of Auburn University Department of Agronomy and Soils and Gazaway is a Professor of Auburn University Department of Plant Pathology. Information contained herein is available to all persons regardless of race, gender, or national origin. 1997 FusARIm WILT TEST E. V. SMrH RESEARCH CENTER, TALLASSEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 1 W.P. Sappenfield, 115 Mango Cove, Leesburg, FL 34748 001 WPS 1..............9 48 11 17 21 002 WPS 2..............................6 55 57 22 35 003 W PS 3 ............................................................... 8 57 54 28 37 004 WPS 4...............35 32 20 29 29 005 ROWDEN.............................71 88 77 86 81 006 W PS 5 ............................................................... 35 45 75 49 51 007 W PS 6 ............................................................... 31 57 57 35 45 008 W PS 7 ................................................................ 17 19 52 20 27 009 WPS8.............................. 2 10 60 39 27 010 M-315.0 15 48 24 22 2 John Green, Seed Source Inc., P.O. Box 28, Stoneville, MS 38776 011 SS1............................... 40 17 96 44 49 012 SS 2 .................................................................. 18 27 24 11 20 013 SS 3 .................................................................. 49 41 44 18 38 014 SS4...........13 38 32 13 24 015 ROWDEN0........................90 93 54 60 74 016 SS 5 .................................................................. 71 38 66 57 58 017 SS 6 .................................................................. 33 39 59 35 42 018 SS 7 .................................................................. 15 42 47 76 45 019 SS 8 .................................................................. 7 9 31 26 18 020 M-315............................. 15 17 13 25 17 3 Shelby H. Baker, Univ. of Georgia, Coastal Plain Station, P.O. Box 748, Tifton, GA 31793 021 G A 1 ................................................................. 53 33 50 22 39 022 G A 2 ................................................................. 57 28 51 41 44 023 G A 3 ................................................................. 41 19 20 36 29 024 G A 4 ................................................................. 11 22 41 46 30 025 ROWDEN.............................92 87 73 58 77 026 GA5 ........................................................ 71 34 49 49 51 027 GA6.................................39 47 51 51 47 028 GA7 ................................................. 46 20 65 32 41 029 GA8 ................................................. 33 33 46 29 36 030 M-315................................................ 31 42 32 18 31 1997 FUSARIUM WILT TEST E. V. SMIrh RESEARCH CENTER, TALLAssEE, ALABAMA Test entry Percent wilt by revlication designation 1 2 3 4 Mean 4 Bob Bridge, Sure Grow Inc., P.O. Box 312, Leland, MS 38756 031 SG 1................................................. 59 78 51 35 56 032 SG 2.........................*0-0#00*0 60*0 0069600096 069 43 79 68 65 033 SG3 ............................................ 32 35 55 25 37 034 SG4 ................................................ 32 25 42 56 39 035 ROWDEN ......................... 0060000000100 49 74 94 79 036 SG 5........ ...................... **oo 000 *a000026 32 51 34 36 037 SG 6.......... ........... 17 22 52 42 33 038 SG 7.......... ............ 13 28 56 37 33 039 SG 8.................................................. 14 39 43 36 33 040 M-315.................17 29 29 15 22 5 Don Keim, Delta and Pine Land Co., 100 Main Street, Scott, MS 38772 041 5863................................. 17 28 43 75 41 042 28429................................................. 4 28 34 23 22 043 6314.................................................. 32 38 58 30 39 044 6065 ...............go.s*...............6037 36 62 33 42 045 ROWDEN...............78 72 94 84 82 046 6570 .......... ............ 48 80 88 59 69 047 15947 ........................................ o.........35 31 42 34 35 048 5093 .......... .............. 0..0....................... 45 63 54 35 49 049 5096.................................................. 25 39 46 20 33 050 M-315.................15 27 45 37 31 6 Cindy Green, Delta and Pine Land Co., P.O. Box 1529, Hartsville, SC 29550 051 1 ..o ................................................... 71 30 92 54 62 052 2 ....... o.o......o..o.o0...o......................... ...... 18 22 60 33 33 053 3.......o......o.................. o... o............. ...... 79 75 71 47 68 054 4.........o............................................ 67 43 56 20 46 055 ROWDEN..................... 100 100 90 94 96 056 5 ..................................... i 0. .6* *....*31 68 52 45 49 057 6 ..o........... . ...................................... 12 48 38 45 36 0-58 7....................................------------42 631 60n 43 52 1997 PuSARIUJM WIoLT TEST E. V. SMITH RESEARCH CENTER, TALLAssEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 7 Richard Sheetz, Paymaster Cottonseed, P.O. Box 8, Aiken, TX 79221 061 1.................................................... 25 9 4 60 24 062 2 ............. ............... 17 25 47 19 27 063 3 ..................................................... 0 37 30 2 17 0644 ..................................................... 8 25 4 7 11 065 ROWDEN...............33 100 89 79 76 066 5 ...................................................... 16 30 24 16 22 067 6 ................ ........... 9 23 21 15 17 068 7 ............ ............. 11 36 39 25 28 069 8 ................ .......... 7 14 22 27 17 070 M-315.................0 33 43 35 28 8 Steve Calhoun, Mississippi State Univ., P.O. Box 197, Stoneville, MS 38776 071 1............. ................ 14 69 67 62 53 072 2....................................................... 9 48 35 33 31 073 3 ...................................................... 12 47 43 12 28 074 4 ...................................................... 19 51 40 10 30 075 ROWDEN...............37 91 94 64 72 076 5 ...................................................... 13 31 30 47 30 077 6 ..................................... 0 0 2 40 75 40 39 078 7 .............................................. 5 21 21 33 20 079 8....................................................... 2 33 62 57 39 080 'M-315.................50 28 35 17 33 9 0. Lloyd May, USDA-ARS, 2200 Pocket Road, Florence, SC 29506-9706 08 1 1 ............ ..................... 58 40 29 45 43 082 2 ...................................................... 73 59 57 34 56 083 3...................................... ... 000aa.... 06. 45 37 33 27 36 084 4.........................909861 0*0060.....000.00O....56 34 72 58 55 085 ROWDEN...............74 87 87 77 81 086 5 ...................................................... 75 48 67 67 64 087 6 ................ ..000940 0 at*$*......ee fo..........46 50 57 54 52 088 7 ..................... 31 69 100 73 68 1997 FUSARIUM WILT TEST E. V. SMrrTH RESEARCH CENTER, TALLASSEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 10 Peggy Thaxton, Dept. of Soil & Crop Sci., Texas A&M Univ., College Station, TX 77843-2474 091 PMT-1 ....................... ......................... 15 50 24 67 39 092 PM T-2 ........ ..................................................... 47 41 61 77 57 093 PMT-3 ............................................................... 69 67 93 57 71 094 PMT-4 ....................................................... 23 23 41 35 31 095 ROWDEN .................................. 91 93 84 87 89 096 PMT-5 .......................................................... 16 31 74 41 40 097 PMT-6 ......................................................... 34 67 33 56 48 098 PMT-7 .................................................... 34 57 84 57 58 099 PMT-8 ..................................................... .... 19 16 13 50 24 100 M-315 . ................................ 13 38 33 0 21 11 Jim Mitchell, Paymaster Cottonseed, 2476 Hwy. 130 East, Stuttgart, AR 72160 101 1 ........................... ....................................... 50 38 56 44 47 102 2 ........................................................................ 46 24 84 63 54 103 3 ........................................ 40 83 91 89 76 104 4 ........................................................................... 13 18 28 50 27 105 ROWDEN ............. ............................ 40 93 64 93 72 106 5 .................................................................. 11 26 58 39 34 107 6 ..................................... 25 51 44 26 36 108 7 ........................................................................ 31 72 39 36 45 109 8 ....................................................................... 3 15 21 16 14 110 M-315 ........................................................... 14 26 20 14 19 12 Michael Swindle, Paymaster Cottonseed, 2476 Hwy. 130 East, Stuttgart, AR 72160 111 1 ....................................................................... 19 19 28 3 17 112 2 ....................................................................... 32 21 47 28 32 113 3 ....................................................................... 25 30 38 35 32 114 4 ....................................................................... 55 42 65 27 47 115 ROWDEN .................................................... 65 69 92 95 80 116 5 ........................................................................ 57 58 77 45 59 117 6 ....................................................................... 56 91 88 33 67 118 7 ..................................................................... 52 92 62 41 62 119 8 ..................................................................... 96 88 95 93 93 120 M-315 ............................................................ 33 14 35 28 28 1997 FuSARIUJM WILT TEST E. V. SMITH RESEARCH CENTER, TALLAsSEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mea 13 Laval M. Verhalen, Dept. Of Agronomy, Oklahoma State University, Stiliwater, OK 74078 121 OKLA-1............................ a****e*000000$9666sos 22 34 14 41 28 122 OKLA-2 ...................... 60 45 57 37 50 123 OKLA-3 ............ ....000060"00 ...............*Gee 13 40 71 18 35 124 OKL-A-4.............................................. 17 33 53 6 27 125 ROWDEN...............94 94 96 '81 92 126 OKLA-5 ... "eo *6*69.....................to**.......00000 29 42 34 28 33 127 OKLA-6..........................9000000*es6998of00600954 33 59 26 43 128 OKLA-7 ........ ...... 00as.....a aa0 .*........e 24 16 29 27 24 129 OKLA-8................6 11 24 12 13 130 M-315.................15 5 15 17 13 14 Fred Bourland, University of Arkansas, 115 Plant Science Building, Fayetteville, AR 7270 1 131 ARKi............................. 67 96 80 41 71 132 ARK 2................................................ 45 45 50 32 43 133 ARK3............................. 41 46 15 13 29 134 ARK4 ................................................ 48 37 59 16 40 135 ROWDEN...............87 90 85 66 82 136 ARK 5.............................. 38 77 56 33 51 137 ARK 6................................................ 21 58 47 42 42 138 ARK 7................................................ 33 48 86 45 53 139 ARK 8............................................... 7 13 57 51 32 140 M-315...............12 23 7 32 18 15 Curtis Williams, Paymaster Cottonseed, 2476 Hwy. 130 South, Stuttgart, AR 72160 141 CW1I................................................ 24 55 40 43 41 142 CW 2................................................ 30 79 32 43 46 143 CW 3........ a000 .0........000 a 0..........27 44 32 45 37 144 CW 4................................................ 39 37 26 86 47 145 ROWDEN..............91 90 95 90 91 146 CW5....................3'7 77 A51 A49 6 1997 FUSARIUM WILT TEST E. V. SMITH RESEARCH CENTER, TALIIASSEE, ALABAMA Test entry Percent wilt by replication designation1 2 3 4 Mean 16 Jack E. Jones, JaJo Genetics, 246 Maxine Drive, Baton Rouge, LA 70808-683 1 151 JJ 1.................................................. 21 75 41 40 44 152 JJ 2.................................................... 38 51 56 58 51 153 JJ3 ................................................... 33 31 63 36 41 154 JJ4 ................................................... 56 23 61 31 42 155 ROWDEN...............90 95 95 91 93 156 JJS......................32 40 37 30 35 157 JJ6 ................................................... 35 50 27 59 43 158 JJ7 ................................................... 13 16 18 23 18 159 JJ8 ................................................... 49 28 41 32 38 160 M-315.................20 25 0 17 15 17 Freddie M. Miller,, Terra International, Inc., P.O. Box 171376, Memphis, TN 38187 161 1.............................. 14 50 162 2..................................................... 44 43 163 3..................................................... 25 44 164 4 ..................................................... 60 73 165 RO DN..............87 92 166 5..........................................*0.*00 fe 50 40 167 6 ..............................................*6900800 61 51 168 7 ...................................................... 41 56 169 8 ...................................................... 27 26 170 M-315.................23 15 80 78 56 85 33 51 62 30 40 53 35 55 91 53 81 79 25 48 47 44 51 32 16 36 17 47 29 34 44 29 18 Bill Fagala, Terra International Inc., 612 Centre Grove Circle, Jonesboro, AR 72401 171 1..................................................... 29 25 172 2..................................................... 53 31 173 3..................................600*60**** 06G*asa,.* 00 14 28 174 4 ............... ..................................... 65 75 175 ROWDEN...............100 96 176 5 ...................................................... 18 51 177 6 ...................................................... 41 49 178 7 ...................................................... 64 45 179 8 ...................................................... 94 79 180 M-315.................50 15 24 28 26 41 45 42 56 26 31 90 55 71 87 64 87 20 26 29 17 24 33 39 52 50 42 43 65 11 0 19 1997 FUSARIUM WILT TEST E. V. SMITH RESEARCH CENTER, TALLASSEE. ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 19 Doug Wessel, Delta and Pine Land Co., 1305 N VIP Blvd., Casa Grande, AZ 85222 181 1 ........................................................................ 26 16 18 19 20 182 2 ........................................................................ 50 20 25 14 27 183 3 ........................................................................ 62 23 57 36 45 184 4 ........................................................................ 88 76 88 89 85 185 ROWDEN .......................................................... 88 96 90 90 91 186 5 ............................................... ............. 69 69 36 15 47 187 6 .................................................................. 72 66 38 34 53 20 Daryl Bowman, Crop Science Dept., North Carolina State University, Raleigh, NC 27695 188 NC 72 ...................... ................................ 56 60 54 36 52 21 Donald M. Panter, Stoneville Pedigreed Seed Co., 2409 Commerce Street, Albany, GA 31707 189 D P 1 ................. ........................................... 30 12 16 43 25 190 M-315 ....... ............... .......... 30 13 37 21 25 191 DP 2 ......... ........... ....................................... 27 61 47 52 47 192 DP 3 ............................................................... ............ ... 64 37 20 23 36 193 DP 4 ...................... ............................................ 58 75 57 66 64 194 DP 5 ........................................................... 71 85 59 44 65 195 ROWDEN ....................................................... 100 86 83 62 83 196 D P 6 ................................................................ 44 85 55 67 63 197 D P 7 ................................................................ 17 51 17 13 25 22 Bill Anderson, Stoneville Pedigreed Seed Co., 2409 Commerce Street, Albany, GA 31707 198 BA 1 ................................................................. 68 70 65 7 52 199 B A 2 ............................................................... 92 97 97 88 94 200 M-315 ............................. 34 28 30 17 27 201 B A 3 ............................................. .............. 37 28 19 25 27 202 B A 4 ................................................................ 41 50 40 51 46 203 BA 5 ............................................................... 17 29 48 68 40 204 BA 6 .............................................................. 33 37 56 67 48 205 ROWDEN ..................................................... 76 98 76 79 82 206 BA 7 .............................................................. 53 79 100 62 73 8 1997 FUSARIUM WILT TEST E. V. SMITH RESEARCH CENTER, TALLASSEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 23 Mark Barefield, Stoneville Pedigreed Seed Co., 2409 Commerce Street, Albany, GA 31707 207 M B 1 .............................................................. 40 23 77 48 47 208 M B 2 .............. . .... ............................................... 21 20 60 13 29 209 M B 3 ..... ........... ...................................... 74 74 93 45 72 210 M-315 .......... ......................... ....... 56 33 34 36 40 211 M B 4 ...... ... ......... ............................................ 80 79 95 94 87 212 MB 5 ..................................... 64 95 84 79 81 213 M B 6 ........................................................... 63 83 56 40 61 214 MB 7 .o ............................. 65 78 87 38 67 215 ROWDEN .................................................. 84 95 96 97 93 24 Roger Ward, Stoneville Pedigreed Seed Co., 2409 Commerce Street, Albany, GA 31707 216 RW 1 ................................................................. 19 20 46 44 32 217 RW 2 ................................................................ 39 43 64 89 59 218 RW 3 ................................................................ 40 10 14 65 32 219 RW 4 .............................................................. 45 56 27 70 50 220 M-315 ......................................................... 6 31 28 16 20 221 RW 5 ................................................................ 65 58 69 70 66 222 RW 6 .............................................................. 65 96 92 54 77 25 Timothy Drew, Cotton Seed International, P.O. Box 117, Wee Waa 2388 Australia 223 IF 1003 ............................... ... .......................... 22 36 40 34 33 224 IF 1006 ...........................................................94 06.... 50 73 69 68 65 225 ROWDEN .... ............................... 61 100 86 71 80 9 1997 FUSARIUM WILT TEST E. V. SMITH RESEARCH CENTER, TALLASSEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 26 Terry Weesner, Arizona Processing, Inc., P.O. Box 50609, Phoenix, AZ 85076 226 AZ 1 ..................................... 37 68 68 65 59 227 AZ 2 ............ ...... ...................................... ..... 44 43 29 55 43 228 AZ 3 ................................. ............. .... 24 59 29 61 43 229 AZ 4 ........................ ............... 46 70 39 61 54 230 M-315 .................... ..................................... 12 8 17 20 14 231 AZ 5 .................... ............................................... 43 38 33 30 36 232 AZ 6 ......................................................... 50 24 40 50 41 233 AZ 7 .............................. 23 17 29 30 25 234 AZ 8 ............................................................... 56 61 74 52 61 235 ROWDEN .................................. 90 98 93 81 91 27 Kathryn M. Glass, Dept. of Agronomy and Soils, Auburn University, Auburn University, AL 36849-5412 236 JAJO 9556 ....................................................... 60 36 19 44 40 237 JAJO 9569 ....................................................... 24 46 48 33 38 238 Stoneville 3M 002 ............................................. 78 42 47 53 55 239 Suregrow 821 ................................................. 22 37 34 43 34 240 M-315 ......................................................... 6 10 38 35 22 10