Agronomy and Soils Departmental Series No. 199 December 1996 Alabama Agricultural Experiment Station Auburn University, Alabama 9 9 6 Dr. James E. Marion, Director ationa I Fusarium Cotton Cotton..... Report 1996 NATIONAL FUSARIUM WILT COTTON REPORT 1 K. M. Glass and W. S. Gazaway 2 Cotton cultivars and elite breeding lines submitted by 21 cooperators were evaluated for fusarium wilt resistance under field conditions at the E. V. Smith Research Center, Plant Breeding Unit, Tallassee, Alabama. These entries were grown on an Independence loamy fine sand highly infested with both the fusarium wilt fungus (Fusarium oxysporum) Schlect. f. vasinfectum [Atk.] (Snyd. & Hans.) and root-knot nematodes (Meloidogyne incognita.). Plots were 40-inch-wide rows, 20 feet in length, separated by 5-foot alleys. Four replications of the test entries and checks, arranged in a block design, were evaluated. Both susceptible (Rowden) and resistant (M-315) cultivars were included as checks. Rowden was planted in row 5 and every tenth row thereafter (15, 25,...,205) and M-315 in row 10 and every tenth row thereafter (20, 30,...,2 10) throughout the test. Plots were planted May 17. Initial plant counts were made on June 14. Wilted plants were counted and removed on July 12, July 26, and August 21. The remaining live plants were counted and recorded on August 21 . Percent wilted plants were then determined and mean wilting for a given entry calculated. Average wilting of the susceptible Rowden was 8, 3, 7, and 7 percent for the four replications (6 percent average). Corresponding wilt percentages for the resistant check, M-315, were 0, 1, 0, and 1 percent (0.5 percent average). Critical evaluation of a given entry should be made relative to the checks closest to the entry within each replication. 'This report is a joint contribution between USDA-ARS, Crop Science Research Laboratory, Mississippi State, Mississippi, and the Alabama Agricultural Experiment Station, Auburn University, Alabama. 2 Research Assistant of Agronomy and Soils and Professor and Extension Plant Pathologist/Nematologist. Evaluation of breeding process or evaluation of entries over years should be made only between the relative value of this entry and that of the closest susceptible check rows for each year. In 1994, a soil analysis for nematodes revealed that southern root-knot (Meloidogyne incognita) and lance (Hoplolaimus galeatus) are two predominant nematode species in the test plots. High populations of both species are found throughout the test area. Other nematode genera present are stubby root (Trichodorus sp.) and stunt (Tylenchorhynchus sp.). Root-knot nematodes, however, appear to be causing the major damage to cotton in the Fusarium Wilt Test as indicated by the high galling indices found on the roots of all cotton lines. Root-knot nematode damage was unusually light on cotton roots of all varieties including the susceptible cultivar, Rowden. Cooler than normal temperatures in the spring and early fall may have reduced root-knot nematode populations as well as the incidence of Fusarium wilt. Root-knot nematode damage to the cotton in other areas of south and central Alabama was also extremely light. Entries submitted by Kathryn Glass are commonly grown cultivars or advanced commercial materials and are listed by name. Entries submitted by other cooperators are listed by their coded numbers. Additional information regarding the genetic background of a specific coded entry should be obtained from the named cooperator. Information contained herein is available to all persons regardless of race, color, sex, or national origin. 1996 FusARium WILT TEST E. V. SMITH RESEARCH CENTER, TALLASSEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 1 Donald M. Panter, Stoneville Pedigreed Seed Co., Inc., P.O. Box 167, Stoneville, MS 38776 001 DMP1I................................................... 0 0 0 0 0 002 DMP2 ................................................... 0 2 0 0 0.5 003 DMP3 ................................................... 2 0 0 0 0.5 004 DMP4 ................................................... 0 1 0 0 0.3 005 ROWDEN..............................*0ooooosoooosoooo 2 1 5 0 2 006 DMP5 ................................................... 0 0 0 0 0 007 DMP6 ................................................... 0 0 0 0 0 008 DMP7 ................................................... 0 1 4 4 2 009 DMP8 ................................................... 0 0 0 0 0 010 M-315.................................0000090000000000oe9* 0 0 2 0 0.4 2 Freddie M. Miller, Terra International, Inc., P.O. Box 171376, Memphis, TN 38187 011 1 .......................................................... 0 0 2 0 0.4 012 2 .......................................................... 0 0 1 0 0.3 0133 .......................................................... 0 0 0 0 0 014 4........................................... 0 0 0 0 0 015 ROWDEN................4 6 11 7 7 0165 ........................................... 0 0 0 0 0 0176 ........................................... 0 0 0 0 0 0187 ........................................... 0 0 2 4 0 019 8....o..o.............................. .....0 004 1 1 0 2 020 M-315.................................000000000 60604000 00000 0 0 0 0 0 3 Don Keim, Delta and Pine Land Co., 100 Main Street, Scott, MS 38772 021 DPX 1111 ................... o............................ 0 0 0 0 0 022 DPX 2048 .. ............. o............................... 0 0 2 0 0.4 023 DPX 2166........0................................ ....... 0 0 1 0 0.3 024 DPX 2044 ..0 ............................................ 0 0 0 0 0 025 ROWDEN..............................04 0000000000000 3 0 2 2 2 026 DPX 1979 ........................................ 0...0.0... 0 0 0 0 0 027 DPX 6954 .. .0..0...................................0...... 0 2 2 0 0.8 028 DPX 6926................................................ 0 0 0 0 0 029 DPXOO026 ............................................... 0 2 0 1 0.7 -3- 1996 FusARIUM WiLT TEST E. V. SMIrh RESEARCH CENTER, TALLASSEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 4 C. Wayne Smith, Dept. of Soil & Crop Sci., Texas A&M Univ., College Station, TX 77843-2474 031 CWS1I................................................... 0 0 0. 0 0 032 CWS2 ................................................... 0 1 1 5 2 033 CWS3 ................................................... 0 1 1 0 0.7 034 CWS4 ................................................... 0 1 3 0 1 035 ROWDEN................0 2 2 9 3 036 CWSS5.............................................. 2 0 4 0 1 037 CWS6 ................................................... 0 0 3 0 0.8 038 CWS7 ................................................. 3 2 29 0 8 039 CWS8 ................................................... 0 0 6 4 3 040 M-315.................0 2 2 0 1 5 Terry Weesner, Arizona Processing, Inc., P.O. Box 50609, Phoenix, AZ 85076 041 AZ-i ..................................................... 0 0 0 0 0 042 AZ-2 ..................................................... 0 0 0 0 0 043 AZ-3 ..................................................... 0 0 0 0 0 044 AZ-4 ..................................................... 2 1 0 0 0.9 045 ROWDEN...............48 8 23 2 20 046 AZ-S ..................................................... 0 0 2 0 0.4 047 AZ-6 ..................................................... 14 0 0 0 3 048 AZ-7............................................. ........ 3 0 0 0 0.7 049 AZ-8 ..................................................... 11 0 0 0 3 050 M-315.................................00*000000000000000of 0 0 0 0 0 6 0. Lloyd May, USDA-ARS, Route 1, Box 53 1, Florence, SC 29501-9603 051 1........................................................... 0 3 0 0 0.7 0522 .......................................................... 7 1 3 0 3 053 3 .......................................................... 3 0 0 0 0.8 0544 .......................................................... 0 0 4 0 1 055 ROWDEN................5 2 9 0 4 056 5................. ........... 0 2 3 0 1 05987.....................0 A0 r7 A0 12 -4- 1996 FUSARium WILT TEST E. V. SMITH RESEARCH CENTER, TALLASSEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 7 Laval M. Verhalen, Dept. of Agronomy, Oklahoma State University, Stillwater, OK 74078 061 OKLA-1 ................................................ 0 2 0 0 0.4 062 OKLA-2 ................................................. 0 0 0 0 0 063 OKLA-3 ................................................. 0 0 0 0 0 064. OKLA-4.................................................. 0 2 0 1 0.7 065 ROWDEN...............15 0 20 14 12 066 OKLA-5 ................................................. 0 0 0 2 0.5 067 OKLA-6 ................................................. 0 2 0 0 0.4 068 OKLA-7 ................................................. 0 0 0 0 0 069 OKLA-8 ..................... :.......................... 0 0 0 0 0 070 M-315.................0 0 0 2 0.4 8 A.L. Germany, Stoneville Pedigreed Seed Co. Inc., Box 167, Stoneville, MS 38776, 071 ALG-1 ................................................... 0 1 3 0 1 072 ALG-2 ................................................... 0 0 1 0 0.3 073 ALG-3 ................................................... 2 0 3 0 1 074 ALGA4................................................... 0 0 0 0 0 075 ROWDEN................0 2 0 0 0.6 076 ALG-5 ................................................... 0 0 0 0 0 077 ALG-6....................................... 0 0 0 3 0.6 078 ALG-7 ................................................... 0 0 10 0 2 079 ALG-8 ................................................... 0 0 0 0 0 080 M-315.................0 2 0 11 3 9 Peggy Thaxton, Dept. of Soil & Crop Sci., Texas A&M Univ., College Station, TX 77843-2474 081 MfAR-i1................................................... 2 1 0 0 0.7 082 MAR-2................................................... 0 0 0 0 0 083 MAR-3 ................................................... 0 0 0 0 0 084 MAR-4................................................... 4 0 14 7 6 085 ROWDEN................0 11 21 22 14 086 MLAR-S .................................................. 4 1 20 0 6 087 MAR-6................................................... 0 0 8 5 3 088 MAkR-7 ................................................... 1 1 1 0 1 -5- 1996 FusAmiuM WILT TEST E. V. SMITH RESEARCH CENTER, TALLASSEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 10 Richard Sheetz, Paymaster Cottonseed, P.O. Box 8, Aiken, TX 79221 0911 .......................................................... 0 0 0 0 0 0922 .......................................................... 1 3 0 0 1 0933 .......................................................... 0 0 0 0 0 0944 .......................................................... 0 0 0 0 0 095 ROWDEN...............21 5 0 12 9 0965 .......................................................... 0 0 0 0 0 0976 .......................................................... 0 0 0 0 0 0987 .......................................................... 0 0 0 0 0 099 8 .......................................................... 0 1 0 0 0.3 100 M-315.................................*0 0990*0000060000*0&ese 0 0 1 0 0.2 11 Doug Wessel, Delta and Pine Land Co., 1305 N VIP Blvd., Casa Grande, AZ 85222 101 1 .......................................................... 0 0 2 0 0.6 1022 .......................................................... 0 0 3 3 1 1033 .......................................................... 0 0 0 0 0 1044 .......................................................... 0 0 0 0 0 105 ROWDEN...............13 2 3 2 5 106 5 .......................................................... 0 3 0 0 0.8 107 6........................................... 0 0 1 0 0.3 1087 .......................................................... 0 0 1 6 2 1098 .......................................................... 0 0 0 0 0 110 M-315.................0 0 0 0 0 12 W. P. Sappenfield, 115 Mango Cove, Leesburg, FL 34748 111 WS-1 ................................................... 0 0 1 2 0.7 112 WPTS-2 ................................................... 0 0 1 0 0.3 113 WPS-3 ................................................... 0 0 0 0 0 114 WPTS-4 ................................................... 2 0 0 0 0.3 115 ROWDEN................4 0 15 16 9 116 WPTS-5 ................................................... 0 0 0 8 2 117 WPTS-6 ................................................... 0 0 0 0 0 118 WPTS-7 ................................................... 0 0 0 0 0 119 WPTS-8 ................................................... 0 0 0 0 0 120 M-315.................................000*066009096 00 gese0000 0 0 0 0 0 -6- 1996 FUSARIUM WILT TEST E. V. SMITH RESEARCH CENTER, TALLASSEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 13 John Green, Seed Source Inc., P.O. Box 28, Stoneville, MS 38776 121 SS-1 ........................................................................ 3 2 0 0 1 122 SS-2 ........................................................................ 0 0 0 0 0 123 SS-3 ........................................................................ 2 0 0 0 0.4 124 SS-4 ........................................................................ 0 0 0 0 0 125 R O W D EN ......................................................... 0. 0 6 0 0 1 126 SS-5 ........................................................................ 0 10 0 0 2 127 SS-6 ........................................................................ 0 6 0 0 1 128 SS-7 ........................................................................ 0 0 0 7 2 129 SS-8 ...................................................................... 0 0 1 0 0.2 130 M -315 ................................................................ 2 10 2 0 3 14 Shelby H. Baker, Univ. of Georgia, Coastal Plain Station, P.O. Box 748, Tifton, GA 31793 131 G A 1 ........................................................................ 0 1 0 2 0.7 132 G A 2 ....................................................................... 0 0 5 1 2 133 G A 3 ....................................................................... 0 0 0 0 0 134 G A 4 ....................................................................... 2 0 0 0 0.5 135 R O W D EN .......................... ................................ 0. 0 1 0 3 1 136 G A 5 ..................................................................... 0 0 0 0 0 137 G A 6 ....................................................................... 0 0 3 0 0.7 138 G A 7 ....................................................................... 0 0 0 0 0 139 G A 8 ....................................................................... 4 2 0 11 4 140 M -315 ................................ ................................... 1 2 2 0 1 15 Cindy Green, Delta and Pine Land Co., P.O. Box 1529, Hartsville, SC 29550 141 1 .............................................................................. 8 0 8 0 4 142 2 .............................................................................. 3 0 0 0 0.7 143 3 .............................................................................. 0 0 0 2 0.5 144 4 .............................................................................. 0 0 0 0 0 145 R O W D EN .......................... ................................. 3 2 8 18 8 146 5 .............................................................................. 0 0 0 0 0 147 6 .............................................................................. 0 2 0 0 0.5 148 7 ......................................................................... 0 8 0 2 149 8 ............................................................................ 0 0 6 0 2 150 M -315 ...................................................................... 0 3 0 0 0.6 -7- 1996 FusARium WILT TEST E. V. SMITH RESEARCH CENTER, TALLASsEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 16 Bill Fagala, Terra International Inc., P.O. Box 171376, Memphis, TN 38187 1511 .......................................................... 0 0 0 0 0 1522 .......................................................... 5 3 2 3 3 1533 .......................................................... 0 0 0 0 0 1544 .......................................................... 0 0 1 2 0.7 155 ROWDEN................9 5 9 5 7 1565 .......................................................... 0 2 0 0 0.4 1576 .......................................................... 0 0 0 0 0 1587 .......................................................... 0 0 0 0 0 1598 .......................................................... 0 0 0 0 0 160 M-3 15.................0 0 0 0 0 17 Curtis Williams, Paymaster Technology Corp., Route 2, Box 57, Stuttgart, AR 72160 161 1 .......................................................... 0 0 0 1 0.3 1622 .......................................................... 0 0 0 0 0 163 3 .......................................................... 0 1 0 0 0.3 1644 .......................................................... 0 1 0 1 0.6 165 ROWDEN..............................ooo00000000*ooooo 0 0 0 0 0 1665 .......................................................... 0 0 0 0 0 167 6........................................................... 0 0 0 0 0 1687 .......................................................... 0 0 0 0 0 1698 .......................................................... 0 0 0 0 0 170 M-315.................1 0 0 0 0.3 18 Jim Mitchell, Paymaster Technology Corp., Route 2, Box 57, Stuttgart, AR 72160 1711 .......................................................... 0 3 0 3 1 1722 ........................................................ 1 0 0 1 0.7 1733 .......................................................... 1 0 0 9 2 1744 .......................................................... 1 5 0 0 2 175 ROWDEN................0 7 0 1 2 176 5......................1 0 0 14 4 1776 .......................................................... 0 0 0 1 0.3 178 7 .......................................................... 1 0 0 0 0.3 179 8 .......................................................... 0 0 0 3 0.6 180 M-315.................................0*00000000600000 00 000 0 1 0 0 0.3 -8- 1996 FusAium WILT TEST E. V. SMITH RESEARCH CENTER, TALLASSEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 19 Jack B. Jones, 246 Maxine Drive, Baton Rouge, LA 70808-6-831 181 JJ-1 ....................................................... 0 0 0 0 0 182 JJ-2 ....................................................... 0 0 1 0 0.3 183 JJ-3 ....................................................... 0 0 0 0 0 184 JJ-4 ....................................................... 1 0 0 0 0.3 185 ROWDEN.............................00000000tofee 00000028 0 10 5 11 186 JJ-5 ....................................................... 3 0 1 0 1 187 JJ-6 ....................................................... 0 0 0 1 0.3 20 Daryl Bowman, Crop Science Dept., North Carolina State University, Raleigh, NC 27695 188 NC1 ..................................................... 0 0 0 1 0.3 189 NC 112 .................................................. 0 1 4 0 1 190 M-315.................0 2 0 3 1 191 NC265 .................................................. 0 0 0 0 0 192 NC258................................................... 1 0 0 0 0.3 193 NC 188 .................................................. 13 0 0 0 3 21 Kathryn M. Glass, Dept. of Agronomy and Soils, Auburn University, Auburn University, AL 36849-5412 194 HartzHl1277 ............................................ 0 0 0 0 0 195 ROWDEN................4 0 4 10 4 196 Terra3O2 ................................................ 0 0 0 0 0 197 Terra 366 ................................................ 0 0 2 3 1 198 Suregrowl125............................................ 0 0 0 1 0.3 199 Suregrow 404............................................ 0 0 0 0 0 200 M-315.................0 2 2 0 1 201 HartzHl122O ............................................ 0 0 0 0 0 202 HartzEH 1244 ............................................ 3 0 0 0 0.7 203 Stoneville 474 ........................................... 0 9 2 0 3 204 Stoneville X 47.......................................... 0 2 0 0 0.4 205 ROWDEN................4 1 12 10 7 206 Deltapine NuCotn 33 ................................... 0 0 1 0 0.3 207 Deltapine NuCotn 35 ................................... 0 0 0 4 1 208 HlyPerformer HS 23 .............................. 0 8 0 0 2 209 Hy Performer HS46 .................................... 0 0 0 6 1 Alabama's Agricultural Experiment Station System AUBURN UNIVERSITY ) Main Agricultural Experiment Station, Auburn. LWESTODNE MADISON JAW N 'i' E. V. Smith Research Center, Shorter. M, ARSHAL DE KALB 1. Tennessee Valley Substation, N2 Belle Mina. BLOU ETOWAH 2. Sand Mountain Substation, Crossville. CALHOUN 3. North Alabama Horticulture Substation, JEFFERSON SAINTCUR Cullman. 4. Upper Coastal Plain Substation, TLE EBURNE Winfield. ELY CY RANDO5. Forestry Unit, Fayette County. 6. Chilton Area Horticulture Substation, Clanton. C " I'7. Forestry Unit, Coosa County. 6 8 8. Piedmont Substation, RR7 Camp Hill. A E)RE A9 9. Foresty Unit, Autauga County. ^ 9 10. Prattville Experiment Field, 10 MACON RUSSELL Prattville. ~ONN"DES MTGOMER 11. Black Belt Substation, Marion Junction. DALS A R&- R 12. The Turnipseed-lkenberry Place, , P13. Lower Coastal Plain Substation, oF HENRY Camden. CRNS COFEE DALE 14. Forestry Unit, Barbour County. 16 15. Monroeville Experiment Field, CVINGT~O Monroeville. HOUT 16. Wiregrass Substation, 1 7 GENEVA Headland. 17. Brewton Experiment Field, Brewton. 18. Ornamental Horticulture Substation, Spring Hill. 19. Gulf Coast Substation, Fairhope.