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AGRONOMY AND SOILS DEPARTMENTAL SERIES 
NO. 189
SEPTEMBER 1995
ALABAMA AGRICULTURAL EXPERIMENT STATION
LOWELL T. FROBISH, DIRECTOR
AUBURN UNIVERSITY, ALABAMA
a

1995 REGIONAL COTTON
FUSARIUM WILT REPORT'
K. M Glass and W S. Gazaway'
Cotton cultivars and elite breeding lines submitted by 20 cooperators were
evaluated for fusarium wilt resistance under field conditions at the E. V. Smith Research
Center, Plant Breeding Unit, Tallassee, Alabama. These entries were grown on an
Independence loamy fine sand highly infested with both the fusarium wilt fungus
(Fusarium oxysporum) Schlect. f. vasinfectum [Atk.] (Snyd. & Hans.) and root-knot
nematodes (Meloidogyne incognita.).
Plots were 40-inch-wide rows, 20 feet in length, separated by 5-foot alleys. Four
replications of the test entries and checks, arranged in a block design, were evaluated.
Both susceptible (Rowden) and resistant (M-315) cultivars were included as checks.
Auburn 56 was used as the resistant check in the Regional Fusarium Wilt Test for many
years. However, M-315 is now being used as the resistant check, because it is the most
consistently resistant cultivar available. Rowden was planted in row 5 and every tenth
row thereafter (15, 25,...,265) and M-315 in row 10 and every tenth row thereafter (20,
30,...,270) throughout the test. Plots were planted May 22. Initial plant counts were
made on June 16. Wilted plants were counted and removed on July 6, July 27, and
August 15. The remaining live plants were counted and recorded on August 22.
1
This report is a joint contribution between USDA-ARS, Crop Science Research Laboratory,
Mississippi State, Mississippi, and the Alabama Agricultural Experiment Station, Auburn University,
Alabama.
2
Research Assistant of Agronomy and Soils and Professor and Extension Plant
Pathologist/Nematologist.
-1-
Percent wilted plants were then determined and mean wilting for a given entry
calculated.
Average wilting of the susceptible Rowden was 89, 83, 93, and 93 percent for
the four replications (90 percent average). Corresponding wilt percentages for the
resistant check, M-31 5, were 4, 6, 8, and 9 percent (7 percent average). Critical
evaluation of a given entry should be made relative to the checks closest
to the entry within each replication. Evaluation of breeding process or evaluation
of entries over years should be made only between the relative value of this entry and
that of the closest susceptible check rows for each year.
A soil analysis for nematodes revealed that southern root-knot (Meloidogyne
incognita) and lance (Hoplolaimus galeatus) are two predominant nematode species in
the test plots. High populations of both species are found throughout the test area.
Other nematode genera present are stubby root (Trichodorus sp.) and stunt
(Tylenchorhynchus sp.). Root-knot nematodes, however, appear to be causing the
major damage to cotton in the Fusarium Wilt Test as indicated by the high galling
indices found on the roots of all cotton lines.
Entries submitted by Kathryn Glass are commonly grown cultivars or advanced
commercial materials and are listed by name. Entries submitted by other cooperators
are listed by their coded numbers. Additional information regarding the genetic
background of a specific coded entry should be obtained from the named cooperator.
Information contained herein is available to all persons regardless
of race, color, sex, or national origin.
-2-
1995 FUSARIUM WILT TEST
E. V. SMITH RESEARCH CENTER, TALLASSEE, ALABAMA
Test entry Percent wilt by replication
designation 1 2 3 4 Mean
1 Richard Sheetz, Paymaster Cottonseed, P.O. Box 8, Aiken, TX 79221
001 1 ........................................................................... 43 51 41 71 51
002 2 ........................................................................... 59 36 19 69 46
003 3 ........................................................................... 87 49 53 86 69
004 4 ........................................................................... 8 5 29 37 20
005 ROW DEN ........................................................... 85 74 95 64 79
006 5 ........................................................................... 2 4 10 14 8
007 6 ........................................................................... 4 6 0 29 10
008 7 ........................................................................... 2 3 16 22 11
009 8 ........................................................................... 9 5 16 10 10
010 M -315 .................................................................. 0 0 3 2 1
2 Laval M. Verhalen, Dept. of Agronomy, Oklahoma State University, Stillwater, OK 74078
011 OKLA-1...............................16 9 10 0 9
012 O KL A -2 ............................................................... 0 10 3 8 5
013 OKLA-3 .......................................................... 36 8 32 27 25
014 O K LA -4 ............................................................... 23 14 35 
52 31
015 ROW DEN ........................................................... 97 85 95 98 94
016 OKLA-5..........3 7 14 21 11
017 OKLA -6 ............................................................... 2 13 12 75 25
018 OK LA -7 ............................................................... 10 4 13 59 21
019 OKLA-8. ............................... 0 0 0 10 2
020 M -315 .................................................................. 0 8 12 3 6
3 Fred Bourland, 115 Plant Science Bldg., Univ. of Arkansas, Fayetteville, AR 72701
021 ARK -1 ................................................................. 26 17 23 15 20
022 AR K -2 ................................................................. 8 11 14 23 
14
023 ARK -3 ................................................................. 34 19 65 12 32
024 ARK -4 ................................................................. 46 46 74 16 46
025 ROW DEN ........................................................... 96 83 96 
92 92
026 ARK -5 ................................................................. 40 78 26 80 56
027 ARK-6.................................42 24 3 21 22
028 ARK-7................................................. 96 100 95 85 94
029 ARK-8................................................. 61 92 31 50 59
030 M-315.................................................. 5 6 2 24 9
-3-
1995 FUSARIUM WILT TEST
E. V. SMITH RESEARCH CENTER, TALLASSEE, ALABAMA
Test entry Percent wilt by replication
designation 1 2 3 4 
Mean
4 0. Lloyd May, CPRU, P.O. Box 3039, Florence, SC 29502-3039
031 1 ........................................................ 53- 95 60 73 70
032 2........................................................ 78 94 38 33 61
033 3........................................................ 56 95 62 31 61
034 4........................................................ 43 51 22 26 35
035 ROWDEN ............................................ 51 100 88 94 83
036 5........................................................ 10 63 19 19 27
037 6 ...... ................. 25 65 98 27 54
038 7........................................................ 19 23 70 13 31
039 8........................................................ 15 58 74 58 51
040 M-315 .................................................. 4 5 13 4 6
5 C. Wayne Smith, Dept. of Soil & Crop Sci., Texas A&M Univ., College Station, TX 77843-2474
041 CWS-1 ................................................. 4 12 6 18 10
042 CWS-2................................................. 11 14 6 65 24
043 CWS-3................................... 14 2 27 82 31
044 CWS-4................................................. 19 5 9 59 23
045 ROWDEN ............................................ 91 82 92 93 89
046 CWS-5 ................................................ 100 91 94 100 96
047 CWS-6 ................................................. 6 6 6 23 10
048 CWS-7................................................. 94 71 96 91 88
049 CWS-8................................................. 51 50 33 34 42
050 M-315.................................................. 3 3 2 12 5
6 Peggy Thaxton, Dept. of Soil & Crop Sci., Texas A&M Univ., College Station, TX 77843-2474
051 MAR-i................................................. 60 33 25 47 41
052 MlAR-2................................................. 35 48 72 37 48
053 MlAR-3................................................. 24 55 70 65 54
054 MAR-4................................................. 65 73 48 79 66
055 ROWDEN ............................................ 87 77 98 84 86
056 MAR-S........................... 64 52 80 56 63
05'7 MXAD4AR-............... .046c9 58 26 35
-4-
1995 FUSARIUM WILT TEST
E. V. SMITH RESEARCH CENTER, TALLASSEE, ALABAMA
Test entry Percent wilt by replication
designation 1 2 3 4 Mean
7 Michael Swindle, Jacob Hartz Seed Co., Inc., P.O. Box 946, Stuttgart, AR 72160'
061 1 ..................................... 83 15 6 50 39
062 2........................................................ 90 4 11 62 42
063 3........................................................ 65 51 43 94 63
064 4........................................................ 53 25 100 85 66
065 ROWDEN............................................ 100 67 99 98 91
066 5........................................................ 95 6 100 88 72
067 6........................................................ 24 11 16 86 34
068 7 ........................................................ 8 9 63 32 28
069 8........................................................ 49 27 79 13 42
070 M-315.................................................. 0 10 2 4 4
8 A.L. Germany, Stoneville Pedigreed Seed Co. Inc., Box 167, Stoneville, MS 38776
071 AILG-1 ........................................ 28 96 27 63 53
072 ALG-2.................................................. 3 36 1 5 11
073 ALG-3 ................................................. 79 67 100 67 78
074 ALG-4 ................................................. 81 91 100 84 89
075 ROWDEN ............................................ 90 97 98 98 96
076 ALG-5 ................................................. 83 91 80 71 8
077 ALG-6 ................................................. 39 4 24 33 25
078 ALG-7 ................................................. 26 21 43 100 47
079 ALG-8...................... e*6069 ..go... .... 6 4 3 23 9
080 M-315.................................................. 2 6 15 21 11
9 Donald M. Panter, Stoneville Pedigreed Seed Co., Inc., P.O. Box 167, Stoneville, MVS 38776
081 DMP-1................................................. 13 8 39 22 20
082 DMP-2................................................. 19 54 39 64 44
083 DMP-3................................................. 17 39 90 46 48
084 DMP-4................................................. 58 31 93 14 49
085 ROWDEN ............................................ 91 90 97 91. 92
086 DMP-5................................................. 39 97 33 67 59
08R7 DIMP-6.......................6690 9I2'3
-5-
1995 FUSARIUM WILT TEST
E. V. SMITH RESEARCH CENTER, TALLASSEE, ALABAMA
Test entry Percent wilt by replication
designation 1 2 3 4 Mean
10 Dr. Joel F. Mahill, Germain's Cotton Research, P.O. Box 80247, Bakersfield, CA 93380'
091 GC95-1............................................... O 68 100
092 GC95-2................................................ 62 100
093 GC95-3 ................................................ 84 93
094 GC95-4................................................ 47 70
095 ROWDEN ............................................ 96 100
096 GC95-5 ................................................ 31 65
097 GC95-6 ................................................ 52 51
098 GC95-7 ................................................ 13 9
099 GC95-8 ................................................ 6 18
100 M-315.................................................. 2 0
89
14
38
35
93
47
100
68
10
5
94 88
56 58
21 59
38 47
94 96
58 50
30 58
12 25
38 18
6 3
11 R.R. Bridge, Suregrow Research, P.O. Box 312, Leland, MS 38756
101 SG-1 ........................................... 6 13
102 SG-2 ........................................... 4 33
103 SG-3 ................................... ...'..001 .46 96
104 SG-4 - ...... ....... oo................................. 33 6
105 ROWDEN......o..o.................0.................. 97 96
106 SG-5......o................................. ... ...... .. 9 12
107 SG-6 .................................. ........ o.........33 60
108 SG-7 .............. o.......o....o....................... 72 67
109 SG-8 .......... ................ o......o............o....... 7 39
110 M-315..o.... oo....o..o....................... o............ 18 2
93
6
55
12
88
37
86
100
41
2
19 33
62 26
100 74
31 21
88 92
24 20
38 54
49 72
13 25
20 10
12 Shelby H. Baker, Univ. of Georgia, Coastal Plain Station, P.O. Box 748, Tifton, GA 31793
111 GA-i............................ 16 18 86 4 31
112 GA-2 ........o...o..o....o.............. ....... .... 34 19 89 10 38
113 GA-3 ........... .. ....................... 0............ 43 30 89 71 58
114 GA-4 ............ o........o.............................. 65 21 63 55 51
115 ROWDEN ..... o........o......................... ..... 100 41 100 91 83
116 GA-5 ....00.....0........0..00.................. 0.......... 100 35 100 71 76
117 GA-6-o............. o.................................... 39 33 98 82 63
118 GA-7........ o................... 00. ....... .... 06000... 88 21 60 89 64
119 GA-8 .................... ....0................0..........0.97 13 65 100 69
120 M-315 ...o......o..... ................ 0.................. 3 8 7 23 10
-6-
1995 FUSARIUM WILT TEST
E. V. SMITH RESEARCH CENTER, TALLASSEE, ALABAMA
Test entry Percent wilt by replication
designation 1 2 3 4 Mean
13 Bill Fagala, Terra International Inc., P.O. Box 171376, Memphis, TN 38187
121 1........................................................ 99 23 19 55 49
122 2 ........................................................ 90 .6 4 84 46
123 3 ....................................................... 100 19 8 0 32
124 4 ....................................................... 100 72 90 93 89
125 ROWDEN............................................ 100 93 98 100 98
126 5........................................................ 72 9 67 21 42
127 6........................................................ 28 19 23 39 27
128 7........................................................ 14 10 24 22 17
129 8........................................................ 15 6 64 11 24
130 M-315.................................................. 4 4 3 0 3
14 Freddie M. Miller, Terra International, Inc., P.O. Box 171376, Memphis, TN 38187
131 1........................................................ 19 17 25 47 27
132 2........................................................ 15 74 74 40 51
133 3.......................................... 31 19 92 15 39
134 4........................................................ 38 19 26 18 25
135 ROWDEN ............................................ 87 93 92 99 93
136 5........................................................ 43 48 12 25 32
137 6........................................................ 85 28 37 77 56
138 7........................................................ 20 81 16 75 48
139 8........................................................ 13 50 17 53 33
140 M-315 ................................................... 0 5 14 3 5
15 Cindy Green, Delta and Pine Land Co., P.O. Box 1529, Hartsville, SC 29550
141 1........................................................ 33 24 80 33 43
142 2........................................................ 40 30 43 64 44
143 3........................................................ 21 11 63 14 27
144 4 ..................................................... 00 33 25 76 10 36
145 ROWDEN ............................................ 90 98 100 97 96
146 5 ...................................................... 86 94 84 94 90
14A 7 6..................16 694n 51 57 A48
-7-
1995 FUSARIUM WILT TEST
E. V. SMITH RESEARCH CENTER, TALLASSEE, ALABAMA
Test entry Percent wilt by replication
designation 1 2 3 4 Mean
16 John Green, Seed Source Inc., P.O. Box 28, Stoneville, MS 38776
151 SS9501................................................ 63 99 55 78 74
152 SS9502................................................ 23 56 28 84 48
153 SS9503................................................ 32 69 80 87 67
154 SS9504 ................................................ 6 33 65 34 35
155 ROWDEN ............................................ 96 97 89 100 97
156 SS950S ................................................. 8 32 36 61 34
157 SS9506............................................... 18 12 79 28 34
158 SS9507................................................ 72 17 97 45 58
159 SS9508............................................... 14 37 14 84 37
160 M-315.................................................. 0 0 2 5 2
17 W. P. Sappenfield, 115 Mango Cove, Leesburg, FL 34748
162 AZ-2 .................................................. 8 19 0 24 13
163 AZ-3 .................................... 000a 12 84 15 86 49
164 AZ-4 .................................. .... 68 12 38 75 48
165 ROWDEN ............................................ 80 94 65 97 84
166 AZ-S .................................................. 20 20 43 28 28
167 AZ-6 .................................................. 17 23 39 11 22
168 AZ-7 .................................................. 11 16 38 13 20
169 AZ-8 .................................................. 37 56 41 54 47
170 M-315 ................................................ 0 0 2 2 1
18 Joseph Vasek, Chembred Inc., P.O. Box 1050, Maricopa, AZ 85239-1050
171 CBX 456.............................................. 1 13 18 14 12
172 CBX457 ............................................. 23 0 39 8 17
173 CBX 458.............................................. 0 11 14 40 16
174 CBX 62O ............................................. 22 32 11 59 31
175 ROWDEN ........................................... 87 72 100 90 87
176 CBX 466 ............................................. 89 22 89 54 63
1pypy "r%177 r P CBX47..................8 1 1 139r% 14A 23
-8-
1995 FUSARIUM WILT TEST
E. V. SMITH RESEARCH CENTER, TALLASSEE, ALABAMA
Test entry Percent wilt 
by replication
designation 1 2 
3 - 4 Mean
19 Doug Wessel, Delta and Pine Land Co., 1305 N VIP Blvd., 
Casa Grande, AZ 85 22
181 DW-1 .................................................. 99 87 100 60 86
182 DW-2 .................................................. 90 9 88 54 60
183 DW-3 .................................................. 15 19 31 35 
25
184 DW-4 .................................................. 88 15 94 23 55
185 ROWDEN............................................ 100 72 100 88 
90
186 DW-5 .................................................. 40 11 100 46 
49
187 DW-6 .................................................. 57 27 32 63 
45
188 DW-7 .................................................. 5 5 16 27 13
189 DW-8 .................................................. 51 1 52 36 35
190 M-315.................................................. 3 4 4 12 6
20 Kathryn M. Glass, Dept. of Agronomy and Soils, Auburn University, Auburn University,
AL 36849-5412
191 Hy Performer HS44 ................................ 15 16 6 65 26
192 Hy Performer HS 46................... 7 44 57 35 36
193 Deltapine DP 5409 ................................... 30 11 100 25 41
194 Deltapine DPX 0227 ................................ 10 33 81 78 51
195 ROWDEN ............................................ 66 76 93 '100 .84
196 Terra302 ............................................... 4 64 31 74 44
197 Terra 366 .............................................. 46 10 46 81 46
198 Suregrow 125 ......................................... 23 48 33 88 48
199 Suregrow 404 ......................................... 12 0 6 83 25
200 M-315.................................................. 2 3 43 22 17
201 HartzHl1277 .......................................... 10 9 28 52 25
202 Hartz H1560.......................................... 3 15 17 35 17
203 Stoneville 474......................................... 46 20 86 71 56
204 Stoneville 495......................................... 20 7 53 82 41
205 ROWDEN............................................. 71 47 80 100 75
206 Chembred CB 1233................................... 19 34 17 78 37
207 Chembred CB 232 ................................... 2 30 22 44 25
208 UAPXOO01............................................ 49 59 84 57 63
209 UAPXOO03............................................ 19 66 61 75 55
210 ) M-3 11919.................5 10n 21 0 4
-9-


Alabama's Agricultural
Experiment Station System
AUBURN UNIVERSITY
Main Agricultural 
Experiment Station,
Auburn.
E. V. Smith Research Center,
Shorter.
LUMESTONE ADSON JAC)'SON
1. Tennessee Valley Substation,
Belle Mina.
iM MAR DEKAB 2. Sand Mountain Substation,
Crossville.
EOWAH ca 3. North Alabama Horticulture
BLOUT 
Substation, Cullman.
4. Upper Coastal 
Plain Substation,
/SAINT Winfield.
SCEB 5. Forestry Unit, Fayette County.
CLAY 6., Chilton Area Horticulture 
Substation,
PH Clanton.
7. Forestry Unit, Coosa County.
8. Piedmont Substation,
6 7 8 Camp Hill.
PERRY R9. Foresty Unit, Autauga County.
9 10. Prattville Experiment Field,
.10 McAcc nSSEU 
Prattville.
11. Black Belt Substation,
B" DF Marion Junction.
PB UR 12. The Turnipseed-lkenberry Place,
3LCOx Union Springs.
SHENRY 
13. Lower Coastal Plain Substation,
COFFEE DALE 
Camden.
CONECUH CTO 
16 14. 
Forestry Unit, 
Barbour County.
H 15. Monroeville Experiment Field,
SGENEVA Monroeville.
16. Wiregrass Substation,
Headland.
17. Brewton Experiment Field,
Brewton.
18. Ornamental Horticulture Substation,
Spring 
Hill.
19. Gulf Coast Substation,
Fairhope.