he 1993 Regional Cotton Fusarium Wilt Report Department of Agronomy a -.. .oils Departmental Series No. 173 Alabama Agricultural Experiment Station Auburn University Lowell T. Frobish, Director Auburn Unih,rsity November 1993 is -J~b _11 -~ I Ib _,C~g~gq~glsble1llM1IM 1993 REGIONAL COTTON FUSARIUM WILT REPORT 1 K. M. Glass and W. S. Gazaway 2 Cotton cultivars and elite breeding lines submitted by 24 cooperators were evaluated for fusarium wilt resistance under field conditions at the E. V. Smith Research Center, Shorter, Alabama. These entries were grown on an Independence loamy fine sand highly infested with both the fusarium wilt fungus (Fusarium oxysporum) Schlect. f. vasinfectum [Atk.] (Snyd. & Hans.) and root-knot nematodes (Meloidogyne incoqneta.). Plots were 40-inch-wide rows, 30 feet in length, separated by 6-foot alleys. Four replications of the test entries and checks, arranged in a block design, were evaluated. Both susceptible (Rowden) and resistant (Auburn 56) cultivars were included as checks. Auburn 56, developed at Auburn and released in 1953, was used as the resistant check in the Regional Fusarium Wilt Test for many years. However, it was replaced when it was no longer grown commercially. Auburn 56 is again being used as the resistant check, because it is the most consistently resistant cultivar available. Rowden was planted in row 5 and every tenth row thereafter (15, 25,...,275) and Auburn 56 in row 10 and every tenth row thereafter (20, 30,...,270) throughout the test. Plots were planted May 7. Initial plant counts were made IThis report is a joint contribution between USDA-ARS, Crop Science Research Laboratory, Mississippi State, Mississippi, and the Alabama Agricultural Experiment Station, Auburn University, Alabama. 2 Research Assistant of Agronomy and Soils and Professor and Extension Plant Pathologist/Nematologist. on June 2. Wilted plants were counted and removed on June 25, July 9, July 27, and August 24. The remaining live plants were also counted and recorded on August 24. Percent wilted plants were then determined and mean wilting for a given entry calculated. Average wilting of the susceptible Rowden was 93, 85, 94, and 76 percent for the four replications (87 percent average). Corresponding wilt percentages for the resistant check, Auburn 56, were 52, 37, 55, and 37 (45 percent average). Critical evaluation of a given entry should be made relative to the checks closest to the entry within each replication. Evaluation of breeding process or evaluation of entries over years should be made only between the relative value of this entry and that of the closest susceptible check rows for each year. Entries submitted by Kathryn Glass are commonly grown cultivars or advanced commercial materials and are listed by name. Entries submitted by other cooperators are listed by their coded numbers. Additional information regarding the genetic background of a specific coded entry should be obtained from the named cooperator. Information contained herein is available to all persons without regard to race, color, sex, or national origin. [4] 1993 Fusarium Wilt Test E. V. Smith Research Center, Shorter, Alabama Test entry Percent wilt by replication designation 1 2 3 4 Mean 1 C. Wayne Smith, Dept. of Soil & Crop Sci., Texas A&M Univ., College Station, TX 77843-2474 001 WS-1. .................. 98 98 100 100 99 002 WS-2.................... 22 58 71 40 48 003 WS-3 ..................... - 98 95 96 96 004 WS-4................... - 77 95 96 89 005 ROWDEN................. 96 96 94 95 006 WS-5 ................... 80 44 88 75 71 007 WS-6.....................99 96 99 97 98 008 WS-7 ................... 48 80 98 66 73 009 WS-8 ................... 95 74 97 75 85 010 AUBURN 56.......... 37 74 79 28 55 2 James L. Starr, Dept. of Plant Pathology and Microbiology, Texas A & M University, College Station, TX 77843-2474 011 JS-1................... 47 66 41 47 50 012 JS-2 ...................31 65 32 23 38 013 JS-3 ................... 23 43 55 22 36 014 JS-4 ................... 27 55 32 24 35 015 ROWDEN.................88 93 92 94 92 016 JS-5 ....................9 58 51 36 38 017 JS-6............... .... 21 53 71 30 44 018 JS-7................... 12 43 73 50 44 019 JS-8...................26 37 51 19 33 020 AUBURN 56.............. 38 28 41 29 34 3 J. Jefferson Gwyn, Chembred, Inc., 10201 So. 5 1 st Street, Phoenix, AZ 85044 021 CB 232 ................. 36 48 87 83 63 022 CB 333................. 45 65 75 70 64 023 CB 407..................56 63 80 57 64 024 CB 1135 ................ 60 77 69 2 52 025 ROWDEN..................94 93 97 62 87 026 CB 1233.................40 67 27 32 42 027 Acala CB 1210.......... 66 80 82 5 58 028 Acala CB 7............. 39 51 72 30 48 029 Acala CB 305........... 53 38 55 10 39 030 AUBURN 56.............. 55 45 73 28 50 [5] 1993 Fusarium Wilt Test E. V. Smith Research Center, Shorter, Alabama Test entry Percent wilt by replication designation 1 2 3 4 Mean 4 Bobby Phipps, Agrigenetics, 13974 W. Van Buren St. Goodyear, AZ 85338 031 AGC 2006.................86 80 92 14 68 032 AGC 2008............... 60 40 63 18 45 033 AGC 2009............... 88 44 89 24 61 034 AGC 3042................73 25 60 12 43 035 ROWDEN................. 91 96 97 80 91 036 AGC 3043............... 52 55 94 22 56 037 AGC 3049............... 75 88 54 62 70 038 AGC 3015............... 41 66 83 20 52 039 AGC 2005............... 28 56 35 13 33 040 AUBURN 56.............. 15 26 50 16 27 5 Fred Bourland, 115 Plant Science Bldg., Univ. of Arkansas, Fayetteville, AR 72701 041 FB-1 ................... 43 87 68 32 58 042 FB-2 ................... 58 93 87 58 74 043 FB-3 ...................49 73 97 45 66 044 FB-4................. 88 67 90 78 81 045 ROWDEN................. 97 91 97 95 95 046 FB-5 ................... 47 74 81 84 72 047 FB-6 ................... 17 51 92 84 61 048 FB-7.16 43 69 35 41 049 FB-8....................18 75 87 49 57 050 AUBURN 56.............. 32 66 55 63 54 6 John Green, Seed Source, Inc., 106 4th Street, Leland, MS 38756 051 JMG-l a00..................21 43 78 37 45 052 JMG-2...................20 68 62 30 45 053 JMG-3..................22 48 67 53 47 054 ALG-4....................40 78 75 78 68 055 ROWDEN..................93 90 98 65 86 056 JMG-5 .................. 72 58 90 75 74 057 JMG-6...................59 69 79 74 70 058 JMG-7....................98 51 84 62 74 059 JMG-8...................86 73 86 68 78 060 AUBURN 56.............. 92 54 64 51 65 [6] 1993 Fusarium Wilt Test E. V. Smith Research Center, Shorter, Alabama Test entry Percent wilt by replication designation 1 2 3 4 Mean 7 Peggy Thaxton, Dept. of Soil & Crop Sci., Texas A&M Univ., College Station, TX 77843-2474 061 PMT-1.. .................. - 38 55 68 54 062. PMT-2 .................. 99 86 94 95 94 063 PMT-3 ................... 42 75 78 84 70 064 PMT-4................. 78 80 95 84 84 065 ROWDEN.................... 93 93 96 93 94 066 PMT-5.................. 78 96 96 95 91 067 PMT-60..................- 87 92 92 90 068 PMT-70.................. - 97 92 95 95 069 PMT-8 .................. 98 81 93 79 88 070 AUBURN 56.............. 75 41 81 47 61 8 Laval M. Verhalan, Dept. of Agronomy, Oklahoma State Univ., Stillwater, OK 74078-0507 071 OKLA-1.................. 100 79 93 92 91 072 OKLA-2 ................. 68 72 99 80 80 073 OKLA-3................. 51 42 61 42 49 074 OKLA-4 ................. 90 72 92 9 66 075 ROWDEN................. 88 92 88 87 89 076 OKLA-5..................11 66 37 22 34 077 OKLA-6................. 31 41 25 38 34 078 OKLA-7................. 28 63 48 59 49 079 OKLA-8.....................76 70 79 65 72 080 AUBURN 56.............. 42 23 25 36 32 9 R. R. Bridge, Suregrow Research, P.O. Box 312, Leland, MS 38756 081 SG-1 ....................56 74 87 57 68 082 SG-2 ....................45 41 89 65 60 083 SG-3 ....................51 42 90 64 62 084 SG-4 ....................64 19 74 78 59 085 ROWDEN................. 96 90 95 84 91 086 SG-5.......................23 19 68 40 37 087 SG-6..................... 72 74 96 58 75 088 SG-7..................... 61 44 79 29 53 089 SG-8..................... 26 32 78 14 37 090 AUBURN 56.................53 27 79 23 45 [7] 1993 Fusarium Wilt Test E. V. Smith Research Center, Shorter, Alabama Test entry Percent wilt by replication designation 1 2 3 4 Mean 10 Larry Burdett, Delta and Pine Land Co., 1305 N. VIP Blvd. Casa Grande, AZ 85222 091 1. ....................... 76 67 96 73 78 092 2.*...................... 84 54 97 77 78 093 3...................... 100 98 98 69 91 094 4....................... 66 21 85 29 50 095 ROWDEN................. 98 96 95 40 82 096 5 ...................... 92 34 67 6 50 097 6 ...................... 94 56 70 33 63 098 7 ...................... 77 54 58 9 50 099 8.........................40 60 90 49 60 100 AUBURN 56.............. 29 33 40 31 33 11 0. Lloyd May, CPRU, P. 0. Box 3039, Florence, SC 29502-3039 101 L.MAY-1................ 81 69 60 65 69 102 L.MAY-2................ 76 64 68 42 62 103 L.MAY-3....0............. 88 90 67 75 80 104 L.MAY-4. ................. 88 70 94 24 69 105 ROWDEN...................94 94 92 70 88 106 L.MAY-5................ 89 74 64 55 71 107 L.MAY-6. ............... 92 84 86 57 80 108 L.MAY-7................ 78 20 78 43 55 109 L.MAY-8...................89 46 88 50 68 110 AUBURN 56.............. 51 22 84 32 47 12 Richard Sheetz, Cargill Hybrid Seed, Box 2, Aiken, TX 79221 111 1..........................53 10 84 59 51 112 2...........................48 17 69 39 43 113 3 .......................62 12 38 21 33 114 4...........................77 28 60 43 52 115 ROWDEN....................93 51 90 78 78 116 5.........................67 49 84 66 66 117 6........................ 34 21 77 30 40 118 7........................ 52 23 94 76 61 119 8........................ 75 25 100 24 56 120 AUBURN 56................ 60 16 77 80 58 [8] 1993 Fusarium Wilt Test E. V. Smith Research Center, Shorter, Alabama Test entry Percent wilt by replication designation 1 2 3 4 Mean 13 Shelby H. Baker, Univ. of Georgia, Coastal Plain Station, Tifton, GA 31793 121 GA-1................... 97 34 95 70 74 122 GA-2................... 100 47 93 78 79 123 GA-3................... 80 25 90 84 70 124 GA-4................... 93 39 89 68 72 125 ROWDEN...................- 51 94 97 80 126 GA-5. ...................64 8 67 32 43 127 GA-6................... 22 6 49 26 26 128 GA-7 ................... 30 16 55 16 29 129 GA-8 .................... 13 2 41 12 17 130 AUBURN 56.............. 31 14 30 41 29 14 Keith R. Jones, Delta & Pine Land Co., P.O. Box 157, Scott, MS 38772 131 DPL-1 .................. 53 13 34 37 34 132 DPL-2 .................. 70 4 16 25 29 133 DPL-3 ..................95 10 75 44 56 134 DPL-4...................81 6 57 61 51 135 ROWDEN................. 90 44 94 92 80 136 DPL-5 ...................80 6 80 37 51 137 DPL-60..................86 14 94 37 58 138 DPL-7 .................. 99 11 99 50 64 139 DPL-8 ...................67 8 47 31 38 140 AUBURN 56................54 18 41 34 37 15 Bill Falaga, Terra International Inc., P. 0. Box 171376, Memphis, TN 38187 141 1 .......................51 17 66 30 41 142 2...........................65 17 81 63 56 143 3...........................78 10 41 57 47 144 4 .......................72 28 70 46 54 145 ROWDEN................. 95 59 94 61 77 146 5........................ 43 24 70 67 51 147 6........................ 78 30 78 54 60 148 7........................ 89 16 61 61 56 149 8........................ 84 10 84 52 57 150 AUBURN 56.................58 9 71 44 45 [9] 1993 Fusarium Wilt Test E. V. Smith Research Center, Shorter, Alabama Test entry Percent wilt by replication designation 1 2 3 4 Mean 16 Kamal M. El-Zik, Dept. of Soil & Crop Sci., Texas A&M Univ., College Station, TX 77843-2474 151 KME-1.................. 88 14 93 75 68 152 KME-2................... 87 21 85 44 59 153 KME-3................... 77 24 92 35 57 154 KME-4...................86 34 90 70 70 155 ROWDEN.................... 91 67 95 69 80 156 KME-5 .................. 74 19 81 65 60 157 KME-6...................64 27 77 32 50 158 KME-7................... 95 77 100 63 84 159 KME-8.................. 61 26 57 25 42 160 AUBURN 56............... 63 12 55 34 41 17 Curtis Williams, Jacob Hartz Seed Co., Inc., P.O. Box 946, Stuttgart, AR 72160 161 1......................... 76 20 84 77 64 162 2 0..0.................... 70 15 74 56 54 163 3.......................72 14 77 43 52 164 4. .......................56 10 32 14 28 165 ROWDEN..................91 43 90 87 78 166 5 ...................... 56 8 23 37 31 167 6...........................71 20 67 46 51 168 7 .......................33 21 42 30 31 169 8 .......................81 19 72 58 58 170 AUBURN 56.................56 7 51 36 38 18 Jim Mitchell, Jacob Hartz Seed Co., Inc., P.O. Box 946 Stuttgart, AR 72160 171 JM-1 ....................49 4 82 39 44 172 JM-2........................93 48 91 60 73 173 JM-3.......................71 9 86 53 55 174 JM-4......................88 34 96 74 73 175 ROWDEN................. 96 92 97 71 89 [10] 1993 Fusarium Wilt Test E. V. Smith Research Center, Shorter, Alabama Test entry Percent wilt by replication designation 1 2 3 4 Mean 19 D. Steven Calhoun, Dept. of Agronomy, 104 Madison B. Sturgis Hall, Louisiana State Univ., Baton Rouge, LA 70803-2110 176 LA890725............. 64 22 68 10 41 177 LA861875............ 56 11 33 45 36 178 LA861877................ 72 12 40 26 37 179 LA861879 ............... 49 33 38 47 42 180 AUBURN 56.............. 27 15 53 53 37 20 Don Panter, Stoneville Pedigreed Seed Co. Inc., Box 167, Stoneville, MS 38776 181 DMP-1..................69 26 70 56 55 182 DMP-2....... .......... 85 60 93 78 79 183 DMP-3 .................. 100 82 97 72 88 184 DMP-4................... 100 79 98 71 87 185 ROWDEN........... ....... 99 92 99 86 94 186 DMP-5 .................. 100 52 95 58 76 187 DMP-6 .................. 100 70 100 51 80 188 DMP-7....................... 99 50 93 66 77 189 DMP-8.................. 86 84 22 20 53 190 AUBURN 56.................55 59 57 12 46 21 A. L. Germany, Stoneville Pedigreed Seed Co. Inc., Box 167, Stoneville, MS 38776 191 SPSCO-1 .................98 93 94 81 91 192 SPSCO-2..................78 72 62 6 55 193 SPSCO-3 ................. 61 58 50 28 49 194 SPSCO-4................ 30 53 38 46 42 195 ROWDEN...................91 87 91 86 89 196 SPSCO-5..................97 88 97 95 94 197 SPSCO-6.................100 71 97 60 82 198 SPSCO-7................. 99 89 92 67 86 199 SPSCO-8 .................97 50 85 65 74 200 AUBURN 56.................77 39 56 64 59 [11] 1993 Fusarium Wilt Test E. V. Smith Research Center, Shorter, Alabama Test entry Percent wilt by replication desigcrnation 1 2 3 4 Mean 22 Johnie N. Jenkins, Crop Sci. Research Laboratory, P.O. Box 5367, Mississippi State, MS 39762 201 JNJ-1................... 38 12 19 20 22 202 JNJ-233..................39 35 22 7 26 203 JNJ-3 .................. 87 77 75 55 73 204 JNJ-4................... 14 25 8 10 14 205 ROWDEN................... 79 91 94 85 87 206 JNJ-5 .................. 31 47 17 20 29 207 JNJ-6.................... 36 28 13 10 22 208 JNJ-7.................. 33 57 22 21 33 209 JNJ-8*................ 46 59 15 42 40 210 AUBURN 56 .............. 56 44 43 8 37 211 JNJ-9................... 64 54 52 10 45 212 JNJ-10.................. 66 53 49 32 50 213 JNJ-11................. 43 41 38 13 34 214 JNJ-12.................. 90 27 76 18 53 215 ROWDEN.................. 94 85 97 57 83 216 JNJ-13.................73 62 71 13 55 217 JNJ-14................. 52 89 90 23 63 218 JNJ-15................. 92 96 89 43 80 219 JNJ-16...................29 69 29 37 41 220 AUBURN 56..............59 82 52 33 56 23 Freddie M. Miller, Terra International Inc., Box 171376, Memphis, TN 38187 221 1 ...................... 82 91 80 22 69 222 2..........................78 80 96 49 76 223 3. .......................84 38 61 23 52 224 4 ......................-99 100 99 89 96 225 ROWDEN................ 91 95 90 93 92 226 5 .......................93 57 65 33 62 227 6...........................53 42 47 65 52 228 7............-............85 90 76 51 75 229 8 .......................63 54 42 33 48 230 AUBURN 56.............. 34 65 45 18 40 [12] 1993 Fusarium Wilt Test E. V. Smith Research Center, Shorter, Alabama Test entry Percent wilt by replication designation 1 2 3 4 Mean 24 Kathryn M. Glass, Dept. of Agronomy and Soils, Auburn University, Auburn University, AL 36849-5412 231 PD3............. ....... 32 72 52 23 45 232 Stoneville 907......... 62 87 63 27 60 233 Deltapine DP 5415...... 93 66 78 43 70 234 Hyperformer HY 39...... 78 69 79 46 68 235 ROWDEN................. 97 89 91 63 85 236 Suregrow 501.......... 86 81 90 47 76 237 Deltapine 51........... 67 44 84 58 63 238 Chembred CB 1233....... 82 39 91 31 61 239 Georgia King........... 77 91 92 33 73 240 AUBURN 56.............. 39 52 45 40 44 241 Stoneville KC 311 ...... 83 75 40 44 61 242 Hyperformer HS 23. .... 77 57 32 18 46 243 Deltapine DP 5690..... 65 82 52 32 58 244 Terra C 40..............54 85 41 38 55 245 ROWDEN................. 89 96 90 72 86 246 GA 88-88............... 71 93 85 68 79 247 Suregrow 404........... 76 86 61 31 63 248 Hollybrook HB 133 ...... 99 92 92 66 87 249 Stoneville X84-828.'.....91 67 91 57 77 250 AUBURN 56.............. 70 49 53 37 52 251 Stoneville 453......... 97 89 93 78 89 252 SS 9202............. ... 99 80 55 35 67 253 Hyperformer HS 46...... 98 98 97 90 96 254 Deltapine DP 5409...... 99 97 78 40 78 255 ROWDEN.......... ....... 97 98 97 79 93 256 DES 119 ............... 63 93 71 58 72 257 Terra 292.............. 70 87 84 44 71 258 Suregrow 1001.......... 70 92 76 68 76 259 GA 88-15-19............ 96 61 56 43 64 260 AUBURN 56.............. 73 25 36 49 46 261 Chembred CB 1135........91 59 79 40 67 262 Stoneville LA 887.......42 42 64 50 49 263 SS 9303................79 78 84 51 73 264 Deltapine 50........... 75 86 81 48 73 265 ROWDEN................. 97 97 98 59 88 266 Hyperformer HS 44...... 69 86 65 35 64 267 Deltapine Acala 90..... 67 90 66 37 65 268 Terra 207.............. 52 69 65 31 54 269 Suregrow 125........... 78 85 95 34 73 270 AUBURN 56.............. 46 60 45 23 43 271 Stoneville X9573........96 93 100 77 91 272 Hollybrook HB 147.......90 89 83 53 79 273 Deltapine 20........... 75 95 37 32 60 274 Stoneville 132......... 44 47 17 42 38 275 Rowden..................95 96 98 72 90 [13]