A t. .-'~.r * I.. ' AGRONOM~Y AND SOILS DEPARTMENTAL SERIES NO. Soi ALABAMA AGRICULTURAL EXPERIMENT STATION GALE A. BUCHANA,\N, DIRECTOR AUBURN U NIVERSITY. ALABAMA NOVEMBER, 1983 ln/Urination COntained h7erein is availahie to all/persons re,,IardiesS o1 race, color, sexv, or national )riitin. 1983 REGIONAL COTTON FUSARIUM WILT REPORT 1 A. J. Kappelman, Jr. 2 Cultivars and elite breeding lines submitted by 25 cooperators were evaluated for fusarium wilt resistance under field conditions at the Plant Breeding Unit, Tallassee, Alabama. These entries were grown on a Wickham sandy loam soil highly infested with both the fusarium wilt fungus (Fusarium oxvsporum Schlect. f. vasinfectum (Atk.] Snyd. & Hans.) and root-knot nematodes (Meloidogyne spp.) Plots were 40-inch wide bedded rows, 30 feet in length, separated by 6-foot alleys. Four replications of the test entries and checks, arranged in a block design, were evaluated. Both susceptible ('Rowden') and resistant ('McNair 235') cultivars were included as gradient checks. Rowden was planted in row 5 (15, 25,...,255) and McNair 235 in row 10 (20, 30, ... , 250), and then in every tenth row thereafter throughout the test. Plots were planted May 2 but not thinned to three or four plants per foot until June 8. Initial live plant counts were made on June 14. Wilted plants were counted, removed, and recorded on July 14, August 10, and September 7, and remaining live plants counted and recorded on September 8. Percent wilted plants per plot were then determined and mean wilting for a given entry calculated. The incidence of wilt varied greatly across replications. Average wilting of the susceptible Rowden in Rep 1 was 82.9 percent and varied from 1 This is a progress report for information and guidance of coopera- tors, the interpretation of which may be modified with additional experi- mentation. 2 Research Plant Pathologist, USDA-ARS, Adjunct Associate Professor, Department of Agronomy and Soils, Alabama Agricultural Experiment Station. 2 98.6 to 55.3 percent. The mean incidence of wilting in the resistant check in this replication was 17.4 percent but varied from 3.7 to 42.3 percent; thus, there was no overlap in variation between these two check cultivars. As a result, although the degree of wilting of a given entry may seem high, some differentation should be evident. In contrast to the high wilt expression in Rep 1, in Reps 2, 3, and 4 the mean wilting of Rowden was only 40.2 percent but varied from a low of 1.4 percent to a high of 97.1 percent wilted plants per row. In these three replications, mean wilting of McNair 235 was 8.0 percent but varied from 0 to 28.1 percent. Therefore, critical evaluation of a given entry should be made relative to the checks closest to the entry within each replication. Evaluation of breeding progress or evaluation of entries over years should be made only between the relative value of this entry and that of the closest suscep- tible check rows for each year. Entries submitted by W. C. Johnson are commonly-grown cultivars or advanced commercial materials, while entries submitted by A. J. Kappelman are recently-released breeding lines. Thus, these entries are listed by name. Entries submitted by other cooperators are listed by their coded numbers. Additional information regarding the genetic background of a spe- cific coded entry should be obtained from the named cooperator. 1983 Regional Cotton Fusarium Wilt Test, Plant Breeding Unit, Tallassee, Alabama Test entry Percent wilt by replication designation 1 2 3 4 Mean Mason Hawkins, Ranger Seed Co., Box 1288, Tahoka, TX 79373 RSC-1 88.4 12.4 0 14.1 28.7 RSC-2 66.2 0 29.1 6.9 25.6 RSC-3 74.3 4.7 7.8 19.4 26.6 RSC-4 67.5 11.4 8.3 5.4 23.2 Rowden 89.0 19.4 73.9 18.2 50.1 RSC-5 29.5 5.2 19.0 15.3 17.2 RSC-6 55.6 10.0 54.5 11.8 33.0 RSC-7 44.3 9.6 22.2 19.0 23.8 RSC-8 58.6 2.3 25.3 6.0 23.0 McNair 235 10.0 0 0 4.0 3.5 Keith R. Jones, Delta & Pine Land Co., PO Box 157, Scott, MS 38772 DPL-1 18.7 14.7 10.0 26.9 17.6 DPL-2 67.2 5.1 24.1 7.1 25.9 DPL-3 31.5 11.0 26.9 12.5 20.5 DPL-4 17.5 15.2 64.7 10.2 27.0 Rowden 81.2 25.8 76.6 13.4 49.2 DPL-5 73.6 10.4 43.8 22.2 37.5 DPL-6 13.8 4.6 5.8 5.6 7.4 DPL-7 21.2 0 33.3 27.1 20.4 DPL-8 15.4 6.6 7.5 14.3 11.0 McNair 235 13.4 12.5 3.7 7.6 9.3 Jerry D. Carroll, Terra Seed Co., Rt. 1, Box 110, Lubbock, TX 79401 JDC-1 27.7 6.7 10.7 15.1 15.0 JDC-2 50.0 3.5 46.9 7.1 26.9 JDC-3 33.7 21.6 17.8 24.5 24.4 JDC-4 39.3 11.7 32.8 30.3 28.5 Rowden 57.3 14.6 42.0 54.1 42.0 JDC-5 6.5 8.0 29.7 67.3 27.9 JDC-6 38.6 6.2 25.9 42.1 28.2 JDC-7 56.0 0 1.4 23.9 20.3 JDC-8 46.5 6.6 1.5 30.0 21.2 McNair 235 14.5 28.0 0 5.1 11.9 John M. Green, Northrup King Co., PO Drawer 272, Leland, MS 38756 JMG-1 65.2 8.5 7.0 36.4 JMG-2 73.6 1.9 2.9 7.5 JMG-3 93.8 27.7 1.7 22.6 JMG-4 34.1 9.7 20.9 24.1 Rowden 96.0 34.9 78.2 79.2 JMG-5 85.1 13.0 70.6 20.7 JMG-6 51.8 14.8 66.2 20.5 JMG-7 9.5 9.9 15.2 2.9 JMG-8 20.0 2.8 43.5 4.2 McNair 235 18.2 18.8 21.5 12.2 29.2 21.4 36.4 22.2 72.0 47.4 38.3 9.4 17.6 17.7 1983 Regional Cotton Fusarium Wilt Test Plant Breeding Unit, Tallassee, Alabama Test entry Percent wilt by reolication designation 2 3 4 Mean L. L. Barton, Rogers Delinted Cottonseed Co., P.O.Drawer 1340, Waco, TX 76703 LLB-1 16.4 26.6 36.7 34.9 28.6 LLB-2 6.5 15.2 56.7 6.2 21.2 LLB-3 32.9 8.4 26.4 11.1 19.7 LLB-4 53.1 13.9 20.7 14.3 25.5 Rowden 55.3 16.2 97.1 67.1 58.9 LLB-5 40.9 4.1 63.2 54.8 40.8 LLB-6 40.5 6.4 32.1 19.6 24.6 LLB-7 92.0 11.4 64.8 26.2 48.6 LLB-8 95.4 10.9 38.3 68.4 53.2 McNair 235 19.4 22.8 6.2 15.6 16.0 L. S. Bird, Dept. of Plant Science, Texas A&M Univ., College Sta. TX 77843 LSB-1 54.1 5.8 3.6 10.7 18.6 LSB-2 53.8 12.1 51.2 29.4 36.6 LSB-3 66.7 13.0 44.9 6.1 32.7 LSB-4 32.5 8.2 25.0 20.0 21.4 Rowden 84.9 9.9 90.9 43.7 57.4 LSB-5 36.5 20.8 32.4 6.3 24.0 LSB-6 50.0 13.2 38.8 9.3 27.8 LSB-7 50.7 18.4 25.0 7.5 25.4 LSB-8 37.5 14.0 10.5 6.1 17.0 McNair 235 16.1 12.1 4.0 7.0 9.8 Jerry L. Baker, Pioneer Hi-Bred International, Inc., Rt. 3, Vernon, TX 76384 PR-I 23.7 27.3 15.4 6.2 18.2 PR-2 13.0 1.6 41.7 9.1 16.4 PR-3 8.9 0 2.5 6.0 4.4 PR-4 42.4 14.5 5.6 13.8 19.1 Rowden 76.8 18.6 73.5 14.1 45.8 PR-5 60.0 18.7 13.7 33.8 31.6 PR-6 21.8 4.0 10.8 15.2 13.0 PR-7 25.6 8.5 1.0 3.8 9.7 PR-8 28.8 15.2 16.4 19.7 20.0 McNair 235 15.9 6.8 12.1 1.9 9.2 Kamal E1-Zik, Dept. of Plant Sciences, Texas A & M Univ., College Sta., TX 77843 KME-1 22.2 2.6 22.4 15.8 15.8 KME-2 31.0 19.1 7.5 18.6 19.0 KME-3 24.0 0 " 4.0 8.1 9.0 KME-4 13.6 2.3 0 40.3 14.0 Rowden 64.4 38.3 48.3 37.3 47.0 KME-5 11.1 12.8 0 36.4 15.0 KME-6 38.7 19.1 23.4 46.3 31.9 1ME-7 86.4 17.4 6.8 42.9 38.4 KME-8 68.8 20.0 12.1 21.4 30.6 McNair 235 38.5 0 17.0 7.8 15.8 1983 Regional Cotton Fusarium Wilt Test Plant Breeding Unait, Tallassee, Alabama Test entry Percent wilt by relication desination 2 3 Robert Re Bridge, MSU Delta Branch Exp. Sta., P0 Box 197, Stoneville, MS 38776 RRBa-1 53.6 2.9 19.3 6.8 20.6 RRB"02 26.9- 11.4 30.0O 12.8 20.3 RRB-3 24.0 15.4 40.0 27.6 26.8 RRB-4 2144 1.5 25.4 23.7 180 Rowden 83.1 25.6 54.8 35.6 49.8 RRB-~5 13.1 11.1 36,4 6-a1 16.7 RRB-6 14.0 6.6 0 23.1 10.9 RRB-7 22.2 10.4 7.9 18.2 14.7 RRB-8 8.3 8.6 13.0 8.2 9.5 MICNai*-r 2 3 5 8.5 17.4 6.9 10.9 Laval*M. Verhalen, Dept. of Agronomy, Oklahoma State Univ. OKLA-1 OKLA-2 OKLA-3 OKTLA-4 Rowden OKLA-5 OKLA-6 OKLA-7 OKLA-8 McNair 235% 22.1l 53.6 43.9 30.4 64.0 24ol 48.0 8.8 43 *8 24.7 2.6 19.1 18.7 6.3. 27.s5 9.,2 1.*6 9.4 29,o 9 4.6 24.4- 0 16.s7 46.2 42.2 8.6 50-00 59.5 6090 3*8 CW. Manning, Stoneville Pedigreed Seed Co., P0 Box 167, SPScO- I SPSCO-2 SPSCO-3 SPSCO-4 Rowden SPSCO-5 38 *5 68.9 39 *5 52 *9 8101 17.1 14 *9 4.5 14.8 34.0 469*4 4.0.*5 17.5 17.2 20.9 13.*0 74.7 7.8 f Stillwater, OK 74078 15.8 18.8 9.8 8.6 24.5 9.8 18.9 2.8 6.5 16.2 22.9 22.3 22 *9 39.6 12.9 29 *6 20.1 35 *0 9.7 Stoneville, MS 38776 13.0 19.4 19.1 21.3 1.9 2190 27.5 23.6 30.3 54.a7 16.8 Richard Percy, Dept. Plant Sci., Texas A & M Univ., College Station, TX 77843 RP-2 RP-3 McNair 235 RPOM4 66.7 27.8 599*4 21.8 25.0 S6e.7 10.1 21. 1 6.0 8.9 0 9.*1 800 11.*8 23.5 7.1 5.3 5.2 3. 1 20o0 32.s6 13. 1 23.o4 10.7 19.4 W. C. Johnson, Dept. Agronomy and Soils, Auburn University, AL 36849 Deltapine NSL 30.4 26.8 6.4 5.2 17.2 Coker 310 54.7 13.5 27.5 14.3 27.5 Delcot 311 16.7 14.1 10.4 2.5 10.9 Rowden 91.8 59.4 66.80 16.9 58.5 - -- -- -- 1983 Regional Cotton Fusarium Wilt Test Plant Breeding Unit, Tallassee, Alabama Test entry Percent wilt by replication designation 1 2 3 4 Mean W. C. Johnson, Dept. Agronomy and Soils, Auburn University, AL (cont.) DES 422 24.3 10.3 15.3 10.4 15.1 Coker 315 18.9 29.5 44.4 16.9 27.4 McNair 220 14.8 26.3 15.9 16.7 18.4 Stoneville 825 11.9 35.2 60.8 14.5 30.6 McNair 235 19.8 0 16.7 7.8 11.1 Stoneville 213 61.9 15.4 4.6 9.7 22.9 Deltapine 41 10.0 8.9 4.9 13.0 9.2 Stoneville 506 23.6 5.6 8.6 14.0 13.0 Coker 3131 3.7 16.9 40.2 12.3 18.3 Rowden 97.0 50.0 27.4 50.0 56.1 Deltapine 150 11.9 30.6 6.7 12.1 15.3 Coker 208 42.4 36.6 2.8 16.6 24.6 Deltapine 90 25.7 6.2 1.9 3.9 9.4 Coker 304 69.7 16.3 16.1 10.7 28.2 McNair 235 8.6 12.8 4.7 2.1 7.0 Stoneville 1181 73.5 29.0 19.6 2.7 31.2 Deltapine 61 35.7 12.5 3.1 4.8 14.0 Deltapine 733 82.4 59.3 11.1 23.9 44.2 Deltapine 102 29.3 45.8 30.4 38.6 36.0 Rowden 86.1 75.0 27.6 21.1 52.4 Aubrey Germany, Bobshaw Pedigreed Seed Co., PO Box 167, Stoneville, MS 38776 BPSCO-1 16.4 22.8 22.9 5.7 17.0 BPSCO-2 13.3 8.0 4.6 4.2 7.5 BPSCO-3 25.8 17.6 9.7 29.3 20.6 BPSCO-4 17.6 24.7 7.0 11.4 15.2 McNair 235 16.7 12.0 7.4 9.5 11.4 BPSCO-5 58.6 25.8 18.2 27.0 32.4 A. G. Douglas, Hollandale Agric. Serv., PO Box 397, Hollandale, MS 38748 HAS-1301 30.2 14.1 6.8 2.9 13.5 HAS-1302 23.7 6.8 14.3 7.1 13.0 HAS-1303 61.7 14.8 1.1 14.7 12.1 Rowden 95.2 9.0 55.0 17.0 44.0 HAS-1304 0 4.0 5.0 7.1 4.0 HAS-1305 0 25.0 25.0 42.1 23.0 HAS-1306 50.0 18.5 48.3 41.7 39.6 HAS-1307 83.3 16.7 11.4 15.7 31.8 McNair 235 5.6 4.1 15.2 8.3 8.3 HAS-1308 0 5.6 0 6.0 2.9 Delbert C. Hess, Cargill Seed Division, PO Box 1630, Plainview, TX 79072 DCH-1 2.9 20.3 1.2 15.0 9.8 DCH-2 5.2 10.6 2.4 20.0 9.6 1983 Regional Cotton Fusarium Wilt Test Plant Breeding Unit, Tallassee, Alabama Test entry Percent wilt by replication designation 1 2 3 4 Mean Delbert C. Hess, Cargill Seed Division, PO Box 1630, Plainview, TX (cont.) DCH-3 15.6 16.5 11.8 2.4 11.6 Rowden 80.6 55o4 24.1 44.3 51.1 DCH-4 226. 16.4 13.2 15.1 16.8 DCH-5 46.9 27.5 7.4 16.9 24.7 DCH-6 36.4 1. 7 16.1 19.4 18.4 DCH-7 20.9 5.1 0 16.0 10.5 McNair 235 42.3 3.9 9.8 3.9 15.0 DCH-8 64.0 14,1 4.7 16.7 24.9 W. P. Sappenfield, Agron. Dept., Univ. of Mo., Delta Ctr., PO Box 160, Portageville, MO 63873 MO-i 34.4 5.8 9.5 12.9 15.6 MO-2 21.8 13.6 0 7.0 10.6 MO-3 18.6 26.8 0 4.2 12.4 Rowden 82.5 91.0 60.8 27.3 65.4 MO-4 24.5 23.1 12.2 2.3 15.5 MO-5 18.6 9.2 6.7 20.5 13.8 MO-6 12.7 . 13.1 2.0 14.6 10.6 MO-7 17.1 9.1 7.3 8.2 10.4 McNair 235 4.8 8.7 7.8 8.4 7.4 MO-8 4.3 4.7 7.2 4.5 5.4 Carl A. Moosberg, Gro Agri. Seed Co., PO Box 1656, Lubbock, TX 79408 GA-I 84.3 2.5 12.8 14.3 28.5 GA-2 18.9 18.3 29.9 23.5 22.6 GA-3 35.2 1.8 22.2 20.9 20.0 Rowden 95.7 86.0 17.1 1.4 50.0 GA-4 34.5 5.2 16.5 20.3 19.1 GA-5 29.9 7.3 2.2 8.1 11.9 GA-6 28.4 24.2 20.7 10.7 21.0 GA-7 83.0 7.0 11.9 28.6 32.6 McNair 235 28.2 6.6 5.4 5.7 11.5 J. B. Weaver, Dept. of Agronomy, Univ. of Georgia, Athens, GA 30602 JBW-1 29.4 24.3 5.4 21.6 JBW-2 19.4 14.3 1.7 11.4 JBW-3 5.2 1.2 12.5 22.4 JBW-4 34.1 6.2 1.7 39.7 Rowden 79.6 39.7 7.6 88.5 JBW-5 6.6 9.8 0 4.3 JBW-6 1.7 10.5 7.4 4.5 JBW-7 39.7 15.0 27.4 2.7 JBW-8 15.5 3.1 25.0 35.1 McNair 235 15.8 4.6 9.6 1.2 20.2 11.7 10.3 20.4 53.8 5.2 6.0 21 o.2 19.7 7.8 ,, ~y rC 1 r? 1983 Regional Cotton Fusarium Wilt Test Plant Breeding Unit, Tallassee, Alabama Test entry Percent wilt by replication designation 1 2 3 4 Mean T. W. Culp, USDA-ARS, PeeDee Expt. Sta., Florence, SC 29501 TWC-1 44.6 36.4 2.5 14.1 24.4 TWC-2 50.9 10.5 3.4 15.0 20.0 TWC-3 90.8 11.0 4.5 12.2 29.6 TWC-4 55.0 25.7 11.1 0 23.0 Rowden 80.7 38.5 10.3 20.0 37.4 TWC-5 75.0 26.6 11.1 4.2 29.2 TWC-6 48.5 11.3 1.4 7.5 17.2 TWC-7 0 22.0 27.7 38.3 22.0 TWC-8 14.3 18.2 7.0 10.8 12.6 McNair 235 4.8 8.4 10.4 9.6 8.3 Loyd Langford, Coker's Pedigreed Seed Co., Rt 1, Box 152, Lubbock, TX 79408 L-1 9.8 28.0 29.2 8.6 18.9 L-2 27.0 18.8 4.5 6.2 14.1 L-3 40.0 38.8 9.3 13.0 25.3 L-4 34.8 21.4 4.7 28.1 22.2 Rowden 98.6 21.5 29.3 12.3 40.4 L-5 3.5 16.3 0 2.3 5.5 L-6 25.7 18.0 33.3 6.9 21.0 L-7 17.3 29.5 11.3 1.5 14.9 L-8 15.3 41.8 28.6 4.0 22.4 McNair 235 14.1 13.3 0 6.8 8.6 R. L. Shepherd, USDA-ARS, Crop Science Res. Unit, Auburn Univ., AL 36849 RLS-1 10.5 21.7 47.6 50.0 32.4 RLS-2 19.2 9.7 0 13.4 10.6 RLS-3 10.9 14.9 7.3 1.1 8.6 RLS-4 11.8 1.2 14.8 10.7 9.6 Rowden 85.5 80.8 38.5 8.1 53.2 RLS-5 2.8 11.3 1.0 3.0 4.5 RLS-6 55.6 12.2 4.6 29.7 25.5 RLS-7 17.7 14.8 8.5 11.1 13.0 RLS-8 18.8 8.4 1.7 16.7 11.4 McNair 235 13.6 3.0 7.0 10.6 8.6 RLS-9 7.1 10.7 5.9 9.7 8.4 RLS-10 16.4 19.6 9.1 6.2 12.8 Henry Webb, Coker's Pedigreed Seed Co., PO Box 340, Hartsville, SC 29550 C-1 10.0 5.4 1.0 23.5 10.0 C-2 59.5 26.3 12.6 52.2 37.6 Rowden 89.8 90.9 4.1 17.7 50.6 C-3 10.8 30.5 1.0 13.3 13.9 C-4 72.0 22.5 15.6 4.5 28.6 C-5 50.6 15.8 24.3 22.7 28.4 1983 Regional Cotton Fusariura Wilt Test Plant Breeding~ Unit, Tailassee, Alabama Test entry Percent wilt by 4cation designation 1 2 3 4 Mean Henry Webb, Coker's Pedigreed Seed Coo, P0 Box.340, Hartsville, SC (cont.) C-6 McNair 235 C'-7 C-8 56.3 3.7 180 33*7 12.5 8.3 11.8 8.*5 11.6 10.*7 2.4 31.a5 20.6 5.0O 12.3 5.4 25.2 6.9 11.1 19.8 Jack E. Jones, Dept. of Agronomy, Louisiana Stat'--e Univ., Baton JJ-1 JJ-2 Rowden JJ-3 JJ-4 JJ..5 JJ-6 McNair 235 JJ-7 J-8 4.9 15.5 8604 46.2 27.o7 31.2 50.00 27*3 48.0 33.8 11.*4 13.4 6208 34.3 35.4- 1s.7 5.7 2.8 15.6 10.6 100 29.3 9.6 0 22.7 1.3 15.5 3.3 4.5 0 28.6 16.4 18.3 9.8 17.7 9.8 17.6 9.9 A. J9 Kappelman, Jr., USDA-ARS, Crop Science Res* Unit, Auburn Auburn OK ? -1 Auburn OK fg-2 Rowden Auburn Auburn Auburn Auburn McNair Auburn Auburn Auburn Auburn Rowden OK f g-3 OK-4 OK-8 OK-' 14 235 73B-1 73B-2 73B-5 73B-12 30.0 37.5 90.8 17.2 1900 5.9 12.4 2907 0 13.0 19.3 48.3 82.4 2.o7 2.* 1 19.06 9.06 30.9 2.9 9.8 13.8 19.4 47.1 0 17.9 1.60 9.8 45. 1 3.6 1399 32*6 8.3 23*1 6.5 1.3 71*1 3.4 0 16.4 8.41 6.11 0 2.8 5.3 4.2 21.4 6.9 15.9 Rouge, LA 70803 5.4 7.5 51.8 26.4 22.8 10.7 24.0 10.3 24.2 14.4 Univ., AL 36849 9.3 12.4 43.0 9.5 14.9 5.7 15.7 13.6 12.0 7.7 22.3 14.2 46 8 ~h~rrrr+ T~TAkk ~hl~nrr ~ rr DA~inmAn~ C~Arrr~ ~~ ~A n I v rrr~rr ~ I rrrr rr -r ru ~ 1U L-~ 11~4