DEPARTMENT OF AGRONOMY AND SOILS AGRICULTURAL EXPERIMENT STATION AUBURN UNIVERSITY DEPARTMENTAL SERIES NO. 52 R. DENNIS ROUSE,DIRECTOR AUBURN,ALABAMA IjEGIONaL COTTON FISAQ~IUM WILT TEST jESULTS 1979 November 1979 1979 REGIONAL COTTON FUSARIUM WILT REPORT' A. J. Kappelman, Jr. 2 Cultivars and elite breeding lines submitted by 20 cooperators were evaluated for fusarium wilt resistance under field conditions at Tallassee, Alabama. These materials were evaluated on a Wickham sandy loam soil which was highly infested with both the fusarium wilt fungus [Fusarium oxysporum Schlect. f. vasinfectum (Atk.) Snyd, & Hans.] and root-knot nematodes (Meloidogyne spp.). Both the susceptible ('Rowden') and resistant ('Stoneville 603 ') cultivars were included as checks. Rowden was planted in row 5 (15, 25, .. ,, 145) and Stoneville 603 in row 10 (20, 30, ..., -140) and then in every tenth row thereafter throughout the test. All plots were bedded, 30 ft in length, spaced 40 in apart, and separated by 6-ft alleys. Four replications of the test entries were arranged in a systematically randomized complete block design. Entries were planted May 3 and thinned to one plant every 3 or 4 inches on June 5. Initial live plant counts were taken June 12. Wilted plants were counted and removed five times during the growing season. Final counts of live plants were made on September 6, Percent wilted plants per plot were determined as the difference between initial and final live plant counts. Mean wilting percentages for a given entry were then calculated. Environmental conditions during the growing season were somewhat unusual. Minimum air temperature was lower than 60 F until May 27 while iThis is a progress report for information and guidance of cooperators, the interpretation of which may be modified with additional experimentation. 2 Research Plant Pathologist, USDA-SEA-AR, Adjunct Assistant Professor, Department of Agronomy and Soils. maximumair temperatures of 90 F or above occurred only on 15 days during the entire test period. In addition, rainfall was somewhat limiting. Regardless of this, a great deal of-wilting occurred although considerable variability was evident, Average wilting over the entire test for Rowden was 75% but wilt incidence .ranged from 21,1 to 100%. The mean incidence of wilting in Stoneville 603 was only 18% but ranged from 0 to 48.1%. Thus-, while extremely resistant or susceptible entries could be easily distinguished, the variation across replications makes an entry with intermediate resistance difficult to classify. Critical evaluation of an check cultivars. Entries submitted by Wiley C, Johnson are commonly grown cultivars or highly advanced commercial materials; therefore, they are listed by name. Entries submitted by other ooperators are listed by their coded number, Additiona information regarding the genetic background of a specific coded entry should be obtained from the given cooperator. Regional Cotton Fusarium Wilt Test Results, 1979 ?.lant Breeding Unit, Tallassee, Alabama Test entry Percet witby re designation 1 2 3 4 Mean Larry L. arton, Rogers Delinted Cottonseed Co., Waco, Texas LLB-l 30.6 16.09 17.8 46.4 27.9 ILB- 2 38.6 23.1 34.9 15.4 28.0 LLB.-3 48.1 15.9 15.3 73.4 38'.2 LLB -4 56 .9 .29.2 27.8 44.4 39.6 Rowden 6.0 24.1 51.5 70.2 51.7 LLB5 50,9 42.2 48.4 53.1 48.7 Keith R Jones, Delta ' & Pin adC~,S cott,- Mississippi DPL-1 DPL-2 DPL-3 ST 603 DPL-5W 26.6 22.5 31.4 59.5. 29.6 23.:1 25,7 30,0 1.0. 6.2- 15.9 44.2- 25.7 3.9 5.0 8.9 17.9 37,.' 36.*0 14.2 20.0 5.8 43.7 35.9 28.5 17.7 14.3 201.1 26.8 Robert R. Bridge, Miss. State University, Stoneville, Mississippi Bridge-l, 7.8 41.9 .1 .4 12.5 15.9 Bridge-2 23.2 15.8 19.1 18.5 19.1 Rowden 53.3 79.3 21.1 100.0 63.4 Bridge3 21.0 23.3 31.0 7.6 20.7 Bridge_4. 6.2 14.3 16.1 10.9 11.9 Bri d a-5I :::::.: -- : !!:I ::- :3_ 2919..-:: - : 16.7 .3 .7 50. C. W. Manning, Stoneville Pedigreed Seed Co., Stoneville, Mississippi Stoneville-l 16.2 2.7 19.4 0 9.6 ST-603. 25.0 37.3 20.7. 5.1 22..0 ,..Stoneville -2 12.4 29.7 11.2 10.5 15.9 Stoneville-3 49.4 33.8 21.9 16.1 30.3 Stoneville-4 40.0 19.7 22.2 4.4 21.6 Stoneville-5 49.4 18.5 10.7 8.3 21.7 Jerry L. Baer, Pioneer 'Hi-Bred: Internat' 1 Inc. , Vernon, Texas Rowden 70.2 84.7 87.9 84.1 81.7 PR-i 69.9 14.5 38.0 21,.2 35.9 PR-2 44.8 32.9 47.6 24.5 37.4 PR-3 26.7 14.3 29.4 10.2 20.1 PR-4 13.1 4.8 11.1 16.1 11.3 ST-603- . 27.6 2.4 6.3 1.2 9.4 PR-S 53.4 21.4 18.5 11.2 26.1 A. L. Germany, Bobshaw Pedigree Seed Co., Stoneville, Mississippi Bobshaw-l 44.7 17.6 5.5 13.6. 20.3 Bobshaw-2 50.7 18.1 14.9 27.3 27.7 Bobshaw-3 31.2 22.4 2.5 3.8 15.0 Rowden 93.3 81.1 25.5 90.7 72.7 Bobshaw-4 .19.5 24.6 4.8 12.7 15.4 Bobshaw--5 49.2 18.0 16.4 7.0 22.7 Test entry Percent wilt byreplication designation 1 2 J. B. Weaver, Jr., Univ. of Georgia, Athens, Georgia JBW-l 28.2 36.1 7.3 9.5 20.3 JBW-2 6.5 18.8 10.4 10.3 11.5 ST-603 10.8 13.4 3.7 0 7.0 JBW-3 47.4 60.3 4.9 23.8 34.1 JBW-4 72.9 37.5 4.6 43.7 39.7 JBW-5 35.6 34.1 11.5 5.3 21.6 Jerry D. Carroll, Delta & Pine Land Co., Lubbock, Texas JDC'-1 97.6. 41.6 29.3 32.0 50.1 Rowden 84,-3 45.1 67. 82.6 69.8 .JDC- 2 30.6 22.2 28.9 42.9 31.1 JDC-3 41.3 82.3 11.9 13.4 37.2 JDC-4 68.8 29.0 34.1 18.4 37.6 J-5 39.3 19,7 41.2 41.0 35.3 Henry Webb, Coker Pedigreed Seed Co., Hartsville, South Carolina ST-603 14,9 26.5 218 2..6 16.5 Coker-l 63.2 19.8 34.8 20.6 34.6 Coker-2 18 20.6 5442 191 28.0 Coker-3 36. I 24.7 20.3 15.9 24.3 Coker-4 24.0 2.0 52.6 b 76 26.1 Rowden 100.0 '89.3 100.0 Q 100.097.3 W. P.::Sappenfied, Univ . of -Mo.Delta Center, Portageville, Missouri Mtn- 5.7 32.4 16.494 M03 6. 21.4 45531. Roger G. Ward, Delta & Pine Land Co., San Joaquin Valley, California -RGW- 2. 43.3 29.3 1 6.7 1.5.9 26.3 ST4-603 21, 2 22.9 16.7 2.8 15.9 1BGW-2 1, 7 19 ,0 9.8 .10.6 10.3: RGW-3 38.6 32.1 6.6 6.1 20.9 RGW-~4 25.3 i 59.0 3.5 10.8. RG5 83.6 55.4 61.3 4.4 51.-2 Rowden 99.0 100.0 93.0 76.0 92.0 Gene Douglas, Hollandale Agr. Service, Hollandale, Mississippi HAS-1801 21.4 18.4 12.5 19.7 18.0 Test entry Percent wilt by replication designation 1 2 3 4 Mean Delbert C. Hess, ACCO Seed, Plainview, Texas ACCO-l 51.5 14.1 30.4 59.3 38.8 Rowden 88.4 84.5 89.0 100.0 90.5 ACCO-2 31.6 33.8 40.2 .76.4 45.5 ACCO-3 61.2 35.7 22.8 82.4 50.5 ACCO-4 31.2 70.2 18.2 24.7 36.1 ACCO-5 64.8 30.6 42.9 52.9 47.8 ST-603 28.0 11.5 12.2 1.9 13.4 Jack E. Jones, Louisiana State University, Baton Rouge, Louisiana JLSU-1 24.4 i32.1 20.2 9.5 21.5 JLSU-2 17.3 12.1 16.4 30.6 19.1 JLSU-3 20.3 8.4 19.0 11.3 14.7 JLSU-4 59.4 28. 0 21.6 41.3 37.6 Rowden. 86:. 95.1 94.2 94.4 92.4 JLSU-5 30.6 13.9 18.7 11.1 18.6 JLJSU-6 27.0 11.4 32.1 3.6 18.5 Laval M. Verhalen, Okia. State Univ., Stillwater, Oklahoma Okla-1 27.8 9.9 10.3 16.7 16.2 Okl.a-2 30.7T 13..1 12.7 39.7 24.1 ST--603 25.7 9.6 27.2 35.1 24.4 Okla-3 612 44.4 38.4 23.3 41.8 Okla-4 1. 16.5 13.0 15.6 14.5 Ok1a 5_ 31.2 .14..3 11.4 15.3 18.1 T. W.: Cuip, USDA, SEA-, AR', Florence, South Carolina Culp-I 27.9 20.5 54.2 17-.3 30.0 RBowden 67.1 63 .8$ 98.'8 53.4 70.8 Culp.-2 17.1 :15.6 36.6 20.4 22.4 Culp-3 2.3.9 34.2 7.2 20.7 21.5 Culp-4 1 6.2 39 .1 6 9.0 2.9 31.8 Culp.-5 85.9 64.2 91.8 18.9 65.2 Wiley..C . Johnson, Auburn University, Auburn,. Alabama ST-603 Coker 310 Dixie King III Stoneville X213 DES-24': Rowden : - lMcNair 235 Brycot 4 McNair 220, Hancock ST--603 Rex 731 Deltapine 55 Coker. 3114 DES-56 Rowden Delcot 277 Vail: 7 Auburn 56 12 *.2 42.0. 17.6 87.. 7 40'.2 92.*6 12. 86.3 7.4 40.7 48.1 35.8 33.*3 35.*2 24.1 36.3 439 23.7 28.6 14.5 36.5 34.-2 51.3 44.7 92.3 13.1 35.6 19,1.I 30 * 8 2.5 20.3 23.2 17.6 29.5 87.7 51.1 60.6. 17.1 27.4 30.9 12.1. 81.8 25.*3 77.4 14- .8 73.4. 14.9 54.5 7.7 14.1 5.5 16. 2 17.*7 78.6 56.5 20.4 25.0 43.0 30.1 29.5 37.0 9.9 80.8 26.9 . 16.3 90.1 17.*1 2.6 9.9 8.3 32.2 89.8 29.4 75.9 40.7 24.3 34.,9 23.3 64.5 30.0 85.*8 16.9 59.8 14.4 54.0 18.8 18.2 18.0 19.3 25.9 73.1 45.2 45.1 27.9 Test entry Percent wilt by replication designation 1 2 3 4 Mean Wiley C. Johnson, Auburn University, continued Coker 420 19.0 18.5 18.0 11.0 16.6 ST-603 16.7 15.4 7.2 4.1 10.9 Coker 3114 19.5 16,9 11.9 1.2 12.4 Deltapine 41 40.5 9.5 16.5 37.4 26.0 Coker 315 59.0 12.5 15.4 16.7 25.9 Deltapine 26 74.4 15.1 13.5 5.5 27.1 Rowden 100.0 47.9 98.8 63.4 77.5 Deltapine 70 64.2 42.5 17.2 9.1 33.3 Coker 304 53.6 12x7 22.4 1.4 22.5 Deltapine 61.29.2 31.2 41.5 5,5 26.9 Stoneville 825 76.1 14.6 100.0 14.1 51.2 ST-603 39,7 7.6 .45.7 17.4 27.6 RR-50 30.0 4.7 40.6 10.1 21.3 James L. Helm, McNair Seed Co., Laurinburg, North Carolina McNair-1 5.1 37.5 6.1 24.1 McNair-2 47.8 5.7 15.9 3.4 18.2 McNair-3 42.9 19.5 19.1 10.6 23.0 Rowden 55.6: 24.0Q 97.6 2097 49.5 McNair 3150 27.9 12.7 23.0 13.4 .19.3 McNair 3151 23.8 .14.7 23.9 14.0 19.1 A. J. Kappelman, Jr. USDA., SEA, AR, Auburn, Alabama AK-1 1.3 11.5: 17.1 8.6 9.6 AK-'2 10.5 24.3 7.9.2 1,2.8 ST-603 25.0: 24.1: 19,5 18.8 21.9 AK-3 30.7 0 11.4 20.8 15.7 AK-4 9.7 6. 6 19.2 11.1 11.7 AK -S 3.3 1 4.9 22.9 1.4 10.6 AK -6 21.31 14.5 21.1 32.12.3 Rowdn 46.7 31.9 44.8 81.1 .11