20o5 Soybean Lesearch Report "~ .4 ~.r 7 '. I ~ I a .~ 4a, Ia a a P ' . r y'I 1 '- - a ~ . 2 d~ ~ ~ ~ * a a -- . . m)fl- ~. .9 .,a<~. .4'" a ~a a~ 1 .. ,. ',' -4. N -. ~'-.- 1' *. .- ' ~~J3 ~1 .~; ~ JI 4 I a. Research Report No. 29 April 2006 Alabama Agricultural Experiment Station Richard Guthrie, Director Auburn University Auburn, Alabama Printed in cooperation with the Alabama Cooperative Extension System (Alabama A&M University and Auburn University) ACKNOWLEDGMENTS This publication is a joint contribution of Auburn University, the Alabama Agricultural Experiment Station, and the USDA Ag- ricultural Research Service. Research contained herein was partially funded from check off dollars through the Alabama Soybean Commission and private industry grants. CONFIDENTIAL REPORT Publication, display, or distribution of data contained herein should not be made without prior written approval. Mention of a trademark or product does not constitute a guarantee of the product by Auburn University and does not imply its approval to the exclusion of other products. Information contained herein is available to all persons regardless of race, color, sex, or national origin. Issued in furtherance of Cooperative Extension work in agriculture and home economics, Acts of May 8 and June 30, 1914, and other related acts in cooperation with the U.S. Department ofAgriculture. The Alabama Cooperative Extension System (Alabama A&M University and Auburn University) offers educational programs, materials, and equal opportunity employment to all people without regard to race, color, national origin, religion, sex. age, veteran status, or disability. CONTENTS page Editors and Contributors................. ................................................ 4.... VARIETY TRIuALs Evaluating Maturity Group III and IV Soybean Varieties at Different Planting Dates, 2005 .................................... 5 Group III and IV Roundup Ready? Soybean Varieties, DeKalb County, 2005 ... ........................... 6 High pH Group IV Roundup Ready? Soybean Variety Trial, Hale County, 2005 .................................................. 7 High pH Group V Roundup Ready? Soybean Variety Trial, Hale County, 2005 ..................................................... 7 Black Belt Group V Conventional Soybean Varieties, Pickens County, 2005 ............................................................ 8 Coffee County Irrigated Group VI Roundup Ready? Soybean Variety Demonstration, 2005 .................................. 8 FUNGICIDE EVALUATIONS Evaluation of Fungicides for Foliar Disease Control and Yield of Soybean in Alabama, 2005........ .......... 9 Evaluation of Absolute, Folicur, and Stratego on Soybean Foliar Disease and Yield in Alabama, 2005............... 10 Evaluation of Punch, Charisma, Folicur, and Manzate on Soybean Foliar Disease and Yield in Alabama, 2005 ........ 11 Evaluation of Ballard for Control of Foliar Diseases of Soybean, 2005 .................................................................. 12 SoYBEAN BREEDING Breeding Improved Soybean Cultivars for Alabama........................................................................................... 13 ALABAMA AGRICULTURAL EXPERIMENT STATION EDITORS D. P. Delaney Extension Specialist IV Agronomy and Soils Auburn University CONTRIBUTORS K. S. Lawrence Associate Professor Entomology and Plant Pathology Auburn University T. Dawkins Superintendent Sand Mountain Research and Extension Center Crossville, Alabama D. R Delaney Extension Specialist IV Agronomy and Soils Auburn University D. Derrick Regional Extension Agent Cherokee County Alabama Cooperative Extension System R. M. Durbin Superintendent Field Crops Unit, E.V. Smith Research Center Shorter, Alabama K. Glass Agricultural Program Associate Agronomy and Soils, Auburn University W. Griffith Regional Extension Agent Fayette County Alabama Cooperative Extension System A. Gutierrez-Estrada Post-doctoral Associate (former) Entomology and Plant Pathology Auburn University K. S. Lawrence Associate Professor Entomology and Plant Pathology Auburn University S. P. Nightengale Superintendent, Plant Breeding Unit E.V. Smith Research Center Tallassee, Alabama R. L. Petcher Regional Extension Agent Washington County Alabama Cooperative Extension System E. J. Sikora Professor and Extension Plant Pathologist Entomology and Plant Pathology Auburn University D. B. Weaver Professor Agronomy and Soils, Auburn University S. Wiggins County Agent Coordinator Pickens County Alabama Cooperative Extension System R. P. Yates Regional Extension Agent Marengo County Alabama Cooperative Extension System VARIETY TRIALS EVALUATING MATURITY GROUP III AND IV SOYBEAN VARIETIES AT DIFFERENT PLANTING DATES, 2005 D. P. Delaney, K. S. Lawrence, E. J. Sikora, S. P. Nightengale, T. Soybeans are traditionally planted in late April through June in Alabama, with Maturity Group (MG) V to VIII cultivars. This combination often places the critical blooming and pod fill stage during moisture deficit periods in late summer. Research in Mis- sissippi has shown that when early maturing varieties from MG III and IV are planted early, they mature before soil moisture deficits become critical and outyield later soybeans. Approxi- mately 60 percent of soybeans in Mississippi were planted with early varieties in 2004, but little work has been done in Alabama with this system and adapted varieties. The purpose of this study was to evaluate use of early ma- turing soybean varieties (Maturity Groups III and IV) under Ala- bama conditions, with the goal of avoiding late summer heat and moisture stress. Tests were conducted at the Plant Breeding Unit (PBU) of the E. V. Smith Research Center near Tallassee, Alabama, and at the Sand Mountain Research and Education Center (SMREC) near Crossville, Alabama, in 2005. Four MG III and four MG IV cultivars ranging from 3.3 to 4.9 were planted on each of two planting dates. Four replications in a split-plot design were used with planting dates as main plots. All varieties were planted with conventional tillage in seven 7-inch rows on April 11 and again on April 29 at PBU and on April 11 and April 25 at SMREC, after being delayed about one week due to wet conditions. TABLE 1. PLANTING DATES FOR GROUP III AND IV SOYBEANS, PBU 2005 Planting date Early April Early April Early April Early April Early April Early April Early April Early April Cultivar DG 3392 NRR DG 3390 NRR Pioneer 93M90 RR DP 3861 RR Pioneer 94B13 RR Pioneer 94M41 RR DP 4724 RR DP 4933 RR Yield bulac 53 54 65 62 60 70 68 72 Total height in 24 20 23 26 24 27 24 33 Lowest pod height in 2 1 3 2 2 4 4 5 Dawkins, and K. Glass Plots were maintained weed-free with recommended herbi- cides, and fungicides were applied twice during pod-fill. Bloom and maturity dates, plant height at initial bloom and maturity, and height to the lowest pod were also recorded during the sea- son. Each treatment was harvested at maturity, from September 2 to September 20 at PBU, and September 13 to September 20 at Sand Mountain. Yields were adjusted to 13 percent moisture and 60 bushels per acre (Tables 1 and 2) and samples taken for seed quality analysis. Yields were generally very good, due to plentiful rainfall in spring and early summer in 2005. At PBU, yields improved from the first to the second planting for most cultivars. For the late planting date, average yields of MG IV varieties were sig- nificantly greater that MG IIIs (76 vs 60 bushels per acre), but they were not different for the early planting. Total height also increased from the first to the second planting but was more pro- nounced for later maturing varieties. There was no significant difference between planting dates for height to the lowest pod, which can affect harvesting ability, in contrast to results from 2004. At SMREC, heavy rains and cool weather after the second planting resulted in a thin stand for that date. Yields were greater for the first planting date, and total height was slightly greater TABLE 2. PLANTING DATES FOR GROUP III AND IV SOYBEANS, SMREC 2005 Planting date Early April Early April Early April Early April Early April Early April Early April Early April Cultivar DG 3392 NRR DG 3390 NRR Pioneer 93M90 RR DP 3861 RR Pioneer 94B13 RR Pioneer 94M41 RR DP 4724 RR DP 4933 RR Yield bu/ac 73 74 87 83 74 86 83 84 Total height in 35 31 34 32 34 38 36 43 Lowest pod height in 3 2 2 4 2 5 4 6 Late April DG 3392 NRR Late April DG 3390 NRR Late April Pioneer 93M90 RR Late April DP 3861 RR Late April Pioneer 94B13 RR Late April Pioneer 94M41 RR Late April DP 4724 RR Late April DP 4933 RR LSD (P=.10) Planting Date Means Early April Late April LSD IP= 101 65 56 61 59 72 75 79 80 13 63 68 4 28 24 26 27 27 30 30 40 3 25 29 1 2 2 2 2 3 4 4 5 1 3 3 NS Late April DG 3392 NRR Late April DG 3390 NRR Late April Pioneer 93M90 RR Late April DP 3861 RR Late April Pioneer 94B1 3 RR Late April Pioneer 94M41 RR Late April DP 4724 RR Late April DP 4933 RR LSD (P=.10) Planting Date Means Early April Late April LSD IP=.10) 63 59 53 60 57 65 70 53 10 81 60 5 36 30 29 31 32 34 33 40 3 35 33 1 4 4 1 . :1 1-I M-%F SW A a -0 a %F I -W 5 9 Is WAIF ftM VA9F MW v vv o ALABAMA AGRICULTURAL EXPERIMENT STATION for the first planting date, but there was no difference between IV cultivars at either planting date. Generally, excellent growing planting dates for height of the lowest pod. There was no sig- conditions allowed even very early blooming and maturity treat- nificant difference between average yields of MG III and MG ments to grow well and produce excellent yields in 2005. GROUP III AND IV ROUNDUP READY? SOYBEAN VARIETIES, DEKALB COUNTY, 2005 D. P. Delaney and D. Derrick Twenty-four Maturity Group (MG) III and IV soybean cul- tivars, all Roundup Ready?, were planted on May 10, 2005 with cooperator Allen Duke in DeKalb County, Alabama. All plots were six 30-inch rows 15 feet wide and approximately 300 to 500 feet long. MG IV varieties were planted with no-till into a heavy bigflower vetch cover crop. Due to limited space, MG III varieties and one early IV were planted in an adjacent area of the field where the vetch had been tilled. Rainfall and growing conditions were very good with Head- line SBR fungicide applied at approximately the R3 growth stage. Plots were harvested on September 28, using the produc- er's combine and a weigh wagon.Yields were adjusted to 13 per- cent moisture and 60 pounds per bushel (see table). GROUP III AND IV ROUNDUP READY@ SOYBEAN VARIETIES, DEKALB COUNTY, 2005 Company Variety Lodged' Yield bu/ac @ 13 pct Monsanto Asgrow 4703 1 63.9 Dyna-Gro DG 3443 2 63.3 Delta King DK 4366 1 62.6 Progeny Progeny 4401 1 62.5 Pioneer Pioneer 94M90 NA 61.9 Monsanto Asgrow 4801 NA 61.5 AP/Garst Garst 4512 1 60.4 Delta King DK 4763 2 60.3 Monsanto Asgrow 4404 1 59.3 UniSouth USG 7434 1 58.8 Progeny Progeny 4804 3 58.2 Monsanto Asgrow 4403 1 57.8 Delta King DK 4866 1 56.6 AP/Garst Garst 4888 NA 54.2 Progeny Progeny 4949 NA 53.9 Monsanto Asgrow 4903 NA 53.5 Dyna-Gro DG 3463 1 53.4 AP/Garst Garst 484 1 53.3 Croplan RC 4891 2 53.0 Delta King DK 4967 NA 52.1 Croplan RC 4842 1 51.6 Deltapine DP 4646 4 50.7 Deltapine DP 4724 2 49.7 UniSouth USG 7482 2 48.9 Tilled soil AP/Garst Garst 3824 4 57.4 Deltapine DP 4331 1 54.8 UniSouth USG 7393 1 41.5 1 Lodging ratings: 1 = all plants erect, 3 = all plants leaning 45 percent, 5 = all plants down. 2005 SOYBEAN RESEARCH REPORT 7 HIGH PH GROUP IV ROUNDUP READY* SOYBEAN VARIETY TRIAL, HALE COUNTY, 2005 D. P. Delaney, R. P. Yates, and E. J. Sikora One of the most critical decisions a soybean producer makes each year is which variety to plant. Yield and other traits such as disease resistance can vary substantially between varieties in response to their environment. On-farm field trials are important to verify university research and to show how different varieties perform under typical producer management practices. Eleven Maturity Group IV soybean cultivars, all Roundup Ready@, were planted on May 11, 2005 in Hale County north of Gallion, Alabama, with Chris Elliott, cooperator. Planting was delayed by wet soil in the spring. The Black Belt soil had an ini- tial pH of 7.7 to 7.8 making it prone to iron chlorosis problems. All varieties were planted in 20-inch rows, in strips 20 to 25 feet wide and approximately 1100 feet long, following conventional tillage. All seed was fungicide treated and inoculated before planting. Plots were rated for iron chlorosis on May 27, June 18, and July 14 (see table). Plots were harvested with the producer's combine and a weigh wagon on October 4, with yields adjusted to 13 percent moisture and 60 pounds per bushel. HIGH PH GROUP IV RR? SOYBEAN VARIETIES, HALE COUNTY 2005 I-ron cholorosis Yield Variety Date of rating bulac @ 13 pct May 27 June 18 July 14 Oct 4 Progeny 4804 RR 2 2 1 60.0 Deltapine DP 4546 RR 1 2 1 56.5 Deltapine DP 4724 RR 1 1 1 55.6 DeltaKing DK 4763 RR 6 4 6 55.5 Progeny 4949 RR 2 4 4 54.6 Pioneer 94M90 5 3 2 53.7 Uni-South USG 7482nRR 3 2 1 52.4 DeltaKing DK 4866 RR 4 3 4 51.5 Dyna-Gro 3463NRR 3 3 4 51.0 Croplan RC 4842 RR 0 0 1 50.1 Dyna-Gro 3443NRR 2 1 1 47.2 ' Iron cholorosis rating based on a 0 to 10 scale: 10 = dead, 0 = deep green. HIGH PH GROUP V ROUNDUP READY? SOYBEAN VARIETY TRIAL, HALE COUNTY, 2005 D. P. Delaney, R. P. Yates, and E. J. Sikora One of the most critical decisions a soybean producer makes each year is which variety to plant.Yield and other traits such as disease resistance can vary substantially between varieties in re- sponse to their environment. On-farm field trials are important to verify university research and to show how different varieties perform under typical producer management practices. Eleven Maturity Group V soybean cultivars, all Roundup Ready@, were planted on June 18, 2005 in Hale County near Demopolis, Alabama, on Ken Diller's farm. Planting was de- layed by excessively wet soil in the spring. The Black Belt soil had an initial pH of 7.8 to 7.9, making it prone to iron chlorosis problems. All varieties were planted in eight 30-inch rows, in strips 20 feet wide and approximately 450 feet long, with two replications of each variety. Continued rains slowed early top and root growth of soybeans, while late summer dry weather limited potential. Reliable iron chlorosis ratings could not be made due to the interaction of Septoria brown spot, soil condi- tions, and chlorosis. Both replications of each variety were har- vested together with the producer's combine and a weigh wagon on October 12, with yields adjusted to 13 percent moisture and 60 pounds per bushel (see table). HIGH PH GROUP V RR? SOYBEANS, HALE COUNTY 2005 Company Variety' Yield bu/ac @( 13 pct Deltapine DP 5414 33.6 Croplan RC 5555 32.4 Croplan RC 5892 30.5 Pioneer 95M80 29.1 Deltapine DP 5644 21.0 Croplan RC 4992 16.4 Monsanto AG 5903 15.9 Deltapine DP 5806 13.7 1 All varieties were Roundup Ready. 8 ALABAMA AGRICULTURAL EXPERIMENT STATION BLACK BELT GROUP V CONVENTIONAL SOYBEAN VARIETIES, PICKENS COUNTY, 2005 D. P. Delaney, E. J. Sikora, W. Griffith, and S. Wiggins One of the most critical decisions a soybean producer makes each year is which variety to plant. A problem in Alabama unique to Black Belt soils is iron chlorosis on high pH soils. Variety se- lection is the only practical way to control this problem on these soils. On-farm field trials are important to verify university re- search and to show how different varieties perform under typical producer management practices. Ten conventional (non-GMO) Maturity Group V and late IV soybeans were planted at the Dee River Ranch near Alice- ville, Alabama, on a Black Belt soil. Each variety was planted on May 9 in 12 30-inch rows in field-length blocks of approximate- ly 1 acre each. Growing conditions were generally excellent, as reflected in the yields. Iron chlorosis was not noted in 2005 due to plentiful soil moisture. Plots were harvested in early October, using the producer's combine and yield monitor (see table). CONVENTIONAL GROUP V SOYBEAN VARIETY TRIAL, PICKENS COUNTY 2005 Company Variety Yield bu/ac Public Holladay 91.0 DeltaKing DK 5995 80.6 Public Hutcheson 74.1 Public Anand 66.8 Deltapine DP 5110S 66.2 Progeny 4910 65.0 Deltapine DP 5989 60.0 DeltaKing DP 5989 57.3 AgVenture AVX 53C 53.0 DeltaKing DK 5870 52.0 COFFEE COUNTY IRRIGATED GROUP VI ROUNDUP RADY@ SOYBEAN VARIETY DEMONSTRATION, 2005 D. P. Delaney, E. J. Sikora, and R L. Petcher One of the most critical decisions a soybean producer makes each year is which variety to plant. On-farm field trials are important to verify university research and to show how dif- IRRIGATED GROUP VI ROUNDUP READY@ SOYBEAN VARIETIES, COFFEE COUNTY 2005 Yield Harvest Green Company Variety 1 bulac moisture leaves/ @ 13 pct pct stalks 2 Deltapine DP 6880 50.4 15.9 2 Monsanto AG 6202 46.5 13.8 2 Monsanto H 6255 45.9 13.8 2 Pioneer 96M20 45.8 16.1 3 Deltapine DP 6215 42.3 17.9 4 Delta King B64-51 41.9 14.7 2 Monsanto AG 6702 41.0 14.5 2 Croplan RC 6767 35.3 14.8 2 Croplan RC 6655 33.1 14.8 2 1 All varieties were Roundup Ready. 2 Green leaf/stalk rating 1 = very dry, 3 = some green leaves, 5 = lush/full of moisture. ferent varieties perform under typical management practices in producers' fields. Nine Maturity Group VI soybean cultivars, all Roundup Ready?, were planted with strip-tillage and in-row subsoiling near Ino, Alabama, on the Carnley farm. Seed was planted at ap- proximately 47 pounds per acre with a twin-row (9-inch) planter set on 36-inch main centers on June 9, 2005. Plots were 18 to 36 feet wide, approximately 1400 feet long, and were irrigated and sprayed with fungicides as needed. Plots were harvested with the producer's combine and a weigh wagon on November 4 when pods and seeds were mature. Yields were adjusted to 13 percent moisture and 60 pounds per bushel (see table). As with many soybean fields in Alabama that were sprayed with a strobiluron or pre-mix (strobiluron + triazole) fungicide, leaves and stems remained green well after seed maturity. This caused difficult and slow harvest conditions as well as increased moisture and foreign material in harvested soybeans. Some vari- ety differences were noted (see table). 2005 SOYBEAN RESEARCH REPORT 9 FUNGICIDE EVALUATIONS EVALUATION OF FUNGICIDES FOR FOLIAR DISEASE CONTROL AND YIELD OF SOYBEAN IN ALABAMA, 2005 K. S. Lawrence, D. P. Delaney, E. J. Sikora, and R. M. Durbin A soybean fungicide trial was planted on May 23 at the Plant Breeding Unit of the E. V. Smith Research Center near Tallassee, Alabama. The soil type was a fine sandy loam. Plots consisted of four rows, 25 feet long, with a between-row spacing of 30 inches. Plots were arranged in a randomized complete- block design with four replications. A 10-foot alley separated blocks. The fungicide treatments were applied as a foliar spray at the R1 plant growth stage followed with a second application 21 days later or at the R3 and R5 plant growth stages. Soybean foliar diseases were evaluated by rating incidence and severity of each disease in the plot at the R6 to R7 growth stage. All plots were maintained throughout the season with standard herbicide, insecticide, and fertility production practices as recommended by the Alabama Cooperative Extension System. Plots were harvested on October 1. Data were statistically analyzed using PROC GLM, and means were compared with Fisher's protected least significant difference test (P < 0.05). Weather c ions were favorable for high incidence of foliar disease on soybean as this area endured rains from the tropical storms Cindy as well as the hurricanes Dennis and Ka- trina. Cercospora leaf blight incidence and severity were rela- tively high in 2005; however, no differences between fungicide treatments and the control were observed. Target spot is not fre- quently observed in Alabama although incidence ranged from 31 to 50 percent during this wet summer. Fungicide applications did not reduce incidence or severity of target spot. Maturity and yield were affected by fungicide applications. Plants defoliated earlier in the control (P < 0.05) as compared to the Topsin M plus Quadris fungicide treatment. Yields were also increased over the untreated control by (P < 0.05) the two applications of Topsin M plus Quadris. EVALUATION OF FUNGICIDES FOR FOLIAR DISEASE CONTROL AND YIELD OF SOYBEAN IN ALABAMA, 2005 -Cercospora blight- -Target spot- Yield Treatment Rate Timing incidence severity 2 incidence severity Maturity 3 bulac fl oz/ac Sept 20 Sept 20 Sept 20 Sept 20 Sept 20 Oct 1 Control 50.0 a 4 2.3 a 50.0 a 1.8 a 3.5 a 45.7 b Penncozeb 2 R1 + 21 days 38.7 a 2.0 a 42.5 a 1.5 a 2.8 ab 49.7 ab Topsin M + Folicur 15 + 4 R3 + R5 46.2 a 2.0 a 33.7 a 1.5 a 2.8 ab 48.1 b Topsin M + Quadris 15 + 6.2 R3 + R5 42.5 a 1.9 a 31.2 a 1.6 a 2.3 b 53.2 a LSD (P < 0.05) ns ns ns ns 1.1 4.3 Disease incidence was based on the percentage of plants affected per row. 2 Disease severity was based on percentage of leaf surface area affected of ten leaflets and was rated on a percentage scale: 1= 0-10%, 2 = 11-25%, 3 = 26-50%, 4 = 51-75%, 5 = 75-100%. Maturity was based on the percentage of necrosis and defoliation: 1= 0-10%, 2 = 11-25%, 3 = 26-50%, 4 = 51-75%, 5 = 75 -100%. 4 Means within columns followed by different letters are significantly different according to Fisher's LSD (P < 0.05). ALABAMA AGRICULTURAL EXPERIMENT STATION EVALUATION OF ABSOLUTE, FOLICUR, AND STRATEGO ON SOYBEAN FOLIAR DISEASE AND YIELD IN ALABAMA, 2005 K. S. Lawrence, D. P. Delaney, E. J. Sikora, and S. P. Nightengale A soybean fungicide trial was planted on May 23 at the Plant Breeding Unit of the E. V. Smith Research Center near Tallassee, Alabama. The soil type was a Cahaba loamy sand. Plots con- sisted of four rows, 25 feet long, with a between-row spacing of 30 inches. Plots were arranged in a randomized complete-block design with four replications. A 10-foot alley separated blocks. The fungicide treatments were applied as a foliar spray at the R3 or R5 plant growth stages. Soybean foliar diseases were evalu- ated by rating incidence and severity of each disease in the plot at the R6 to R7 growth stage. All plots were maintained through- out the season with standard herbicide, insecticide, and fertility production practices as recommended by the Alabama Coopera- tive Extension System. Plots were harvested on October 1. Data were statistically analyzed using PROC GLM, and means were compared with Fisher's protected least significant difference test (P < 0.05). Weather conditions were favorable for high incidence of fo- liar disease on soybean as this area endured rains from the tropi- cal storms Cindy as well as the hurricanes Dennis and Katrina. Cercospera leaf blight incidence and severity were moderately high; however, no differences in disease suppression between fungicide treatments and the control were observed. Target spot incidence was also high for this region, and fungicides did not reduce incidence or severity. Rust developed late in the season with the greatest incidence observed in the control plots. Only the Folicur + Stratego applied at the low rate at the R3 stage showed any rust development, which was less than 10 percent of the plot affected. Rust was not observed on any of the other fungicide treatments. Maturity was affected by fungicide ap- plications. Plants defoliated earlier in the control (P < 0.05) as compared to the Folicur + Stratego fungicide treatment applied at the high rate. Yields were not increased by (P < 0.05) the fun- gicide applications. EVALUATION OF ABSOLUTE, FOLICUR, AND STRATEGO ON SOYBEAN FOLIAR DISEASE AND YIELD IN ALABAMA, 2005 -Cercospora blight--- -Target spot- Rust Yield Treatment Rate Timing incidence' severity 2 incidence severity 2 Maturity 3 incidence 2 bulac Untreated 60.0 2.25 57.5 2.50 3.88 a 4 23.7 62.1 Folicur 3.6F SC + 4+ 10 fl ozlac R3 Stratego EC Folicur 3.6F SC + 4 + 7fl ozlac R3 Stratego Folicur 3.6F SC + 4 + 7fl ozlac R5 Stratego Absolute SC 5 fl ozlac R3 Absolute SC 7 fl ozlac R3 Folicur 3.6F 4 fl ozlac R3 58.7 67.5 65.0 58.7 63.7 63.7 1.75 2.38 2.00 2.13 2.25 1.13 56.3 2.00 58.8 1.13 51.3 3.18 b 0 63.1 3.38 ab 2.5 62.7 1.88 3.63 ab 0 64.6 52.5 2.13 3.75 ab 0 66.4 58.8 2.38 3.75 ab 0 62.7 55.0 2.25 3.38 ab 0 66.9 LSD (P < 0.05) 12.4 0.7 13.7 0.7 0.7 14.6 11.0 SDisease incidence was based on the percentage of plants affected per row: 0= 0%, 10 = 1-10%, 20 = 11-20%, 30 = 21- 30%, 40 = 31- 40%, 50 = 41- 50%, 60 = 51- 60%, 70 = 61 - 70%, 80 = 71- 80%, 90= 81- 90%, 100 = 91-100%. 2 Disease severity was based on percentage of leaf surface area affected of ten leaflets and was rated on a percentage scale: 1= 0-10%, 2 = 11-25%, 3 = 26-50%, 4 = 51-75%, 5 = 75-100%. 3 Maturity was based on the percentage of necrosis and defoliation: 1= 0-10%, 2 = 11-25%, 3 = 26-50%, 4 = 51-75%, 5 = 75 -100%. 4 Means within columns followed by different letters are significantly different according to Fisher's LSD (P < 0.05). 10 11 2005 SOYBEAN RESEARCH REPORT EVALUATION OF PUNCH, CHARISMA, FOLICUR, AND MANZATE ON SOYBEAN FOLIAR DISEASE AND YIELD IN ALABAMA, 2005 K. S. Lawrence, D. P. Delaney, E. J. Sikora, and S. P. Nightengale A soybean fungicide trial was planted on May 23 at the Plant Breeding Unit of the E. V. Smith Research Center near Tallassee, Alabama. The soil type was a Cahaba loamy sand. Plots consisted of four rows, 25 feet long, with a between-row spacing of 30 inches. Plots were arranged in a randomized com- plete-block design with four replications. A 10-foot alley sepa- rated blocks. The fungicide treatments were applied as a foliar spray at the R1 plant growth stage followed with a second ap- plication 21 days later. Soybean foliar diseases were evaluated by rating incidence and severity of each disease in the plot at the R6 to R7 growth stage. All plots were maintained through- out the season with standard herbicide, insecticide, and fertility production practices as recommended by the Alabama Coopera- tive Extension System. Plots were harvested on October 1. Data were statistically analyzed using PROC GLM, and means were compared with Fisher's protected least significant difference test (P < 0.05). Weather conditions were favorable for high incidence of foliar disease on soybean as this area endured rains from the tropical storms Cindy as well as the hurricanes Dennis and Ka- trina. Cercospera leaf blight incidence was relatively high with incidence ranging from a low (P < 0.05) of 46 percent in Cha- risma EC at 8 ounces per acre to a high of 62 percent in Punch EC at 5 ounces per acre. However, the severity of the Cercospera blight was low and the fungicide applications did not affect se- verity. Target spot incidence was also high for this region and the Manzate 75 DF did reduce (P < 0.05) incidence as compared to the control. Targot spot severity was not reduced by any fun- gicide treatment. Maturity and yield were affected by fungicide applications. Plants defoliated earlier in the Manzate 75 DF (P < 0.05) as compared to the Punch EC fungicide treatments. Yields were increased (P < 0.05) by the Folicur fungicide treatment as compared to the control, Charisma EC, Manzate, and Punch at 5 ounces per acre. EVALUATION OF PUNCH, CHARISMA, FOLICUR, AND MANZATE ON SOYBEAN FOLIAR DISEASE AND YIELD IN ALABAMA, 2005 -Cercospora blight- -Target spot- Rust Yield Treatment Rate Timing incidence severity 2 incidence severity 2 Maturity 3 incidence buac Sept 20 Sept 20 Sept 20 Sept 20 Sept 20 Sept 20 Oct 1 Untreated control 56.33 ab 4 2.0 60.0 a 2.1 3.2 ab 2.5 56.9 bc Punch EC 400GL 4 fl ozlac R1 + 21 D 57.5 ab 2.1 60.0 a 2.5 2.8 b 5.0 64.7 ab Punch EC 400GL 5 fl oz/ac R1 + 21 D 62.5 a 2.3 58.7 ab 2.4 2.7 b 2.5 54.6 c Charisma EC 207GL 8 fl oz/ac R1 + 21 D 46.3 b 1.4 50.0 ab 2.0 3.6 ab 0 57.5 bc Charisma EC 207GL 10 fl oz/ac R1 + 21 D 57.5 ab 1.8 52.5 ab 2.0 3.5 ab 0 62.0 bc Manzate 75 DF 2 Ib/ac R1 + 21 D 53.8 ab 1.5 42.5 b 2.0 4.0 a 0 62.0 bc Folicur 3.6F 4 fl oz/ac R1 + 21 D 60 ab 2.1 48.7 ab 2.4 3.2 ab 0 70.5 a LSD (P 5 0.05) 15.7 0.9 17.1 0.7 1.01 5.2 8.3 SDisease incidence was based on the percentage of plants affected per row: 0= 0%, 10 = 1-10%, 20 = 11-20%, 30 = 21- 30%, 40 = 31- 40%, 50 = 41- 50%, 60 = 51- 60%, 70 = 61 - 70%, 80 = 71- 80%, 90= 81- 90%, 100 = 91-100%. 2 Disease severity was based on percentage of leaf surface area affected of ten leaflets and was rated on a percentage scale: 1= 0-10%, 2 = 11-25%, 3 = 26-50%, 4 = 51-75%, 5 = 75-100%. 3 Maturity was based on the percentage of necrosis and defoliation: 1= 0-10%, 2 = 11-25%, 3 = 26-50%, 4 = 51-75%, 5 = 75 -100%. 4 Means within columns followed by different letters are significantly different according to Fisher's LSD (P < 0.05). ALABAMA AGRICULTURAL EXPERIMENT STATION EVALUATION OF BALLARD FOR CONTROL OF FOLIAR DISEASES OF SOYBEAN, 2005 E. J. Sikora, D. P. Delaney, K. S. Lawrence, A. Gutierrez-Estrada, and R. M. Durbin A soybean fungicide trial was planted with Pioneer 96M20 on May 24 at the Plant Breeding Unit of the E. V. Smith Re- search Center near Tallassee, Alabama. Plots consisted of four rows, 20 feet long, with a between-row spacing of 30 inches and a seeding rate of 11.7 seeds per foot. Plots were arranged in a randomized complete-block design with four replications. A 10- foot alley separated blocks. The fungicide treatments were ap- plied as a foliar spray on August 1 (R2 growth stage), August 15 (R3-R4), and September 1 (R5). Percent target spot was deter- mined on September 15. Percent leaf defoliation and leaf green- ing (0-3 scale with 3 representing a high level of green foliage being retained by the plant) was determined on October 5.,Inci- dence of Asian soybean rust (ASR) was determined on October 15 by examining five plants per plot. All plots were maintained throughout the season with standard herbicide, insecticide, and fertility production practices as recommended by the Alabama Cooperative Extension System. Plots were harvested on No- vember 10. Weather conditions were somewhat favorable for foliar dis- ease as this area endured rains from tropical storm Cindy as well as the hurricanes Dennis and Katrina. Ballad at 0.5 quart, Ballad at 1 quart + Quadris at 8 ounces, and Quadris at 8 ounces all had less target spot than the control. Quadris at 8 ounces had less ASR incidence compared to all other treatments. Quadris at 8 ounces and Ballad at 0.5 quart had less incidence of ASR compared to the control. There were no significant differences among the other treatments. All treatments with the exception of Ballad at 2 quarts had less leaf defoliation than the control. Bal- lad at 0.5 quart, Ballad at 1 quart + Quadris at 4 ounces, Ballad at 1 quart + Quadris at 8 ounces, and Quadris at 8 ounces had less defoliation and higher levels of leaf greening among treatments. Ballad at 1 quart + Quadris at 8 ounces had higher yields than all other treatments. Quadris at 8 ounces had higher yields than the control and Ballad at 1 or 2 quarts. There were no significant differences in yield among the other treatments. EVALUATION OF BALLARD FOR CONTROL OF FOLIAR DISEASES OF SOYBEAN, 2005 Treatment' Rate Target spot Defoliation Leaf ASR Incidence 3 Yield per acre pct pct qreeninq 2 pct bulac Control 13.5 ab 4 96.5 a 1.0 c 100 a 58.6 c Ballad SC Ballad SC Ballad SC Ballad SC + Quadris SC Ballad SC + Quadris SC Ballad SC + Quadris SC Quadris SC 0.5 qt 6.25 cd 1 qt 2 qt 0.5 qt + 4 oz 1 qt+4 oz 1 qt+ 8oz 8 oz 70.0 d 11.5 abcd 89.25 b 14.25 a 10.5 abc 3.5 c 7.0 bcd 3.25 c 93.75 ab 82.25 c 69.25 d 68.75 d 64.5 d 12 2.75 a 1.25 c 1.0 c 1.75 b 3.0 a 3.0a 3.0 a 60 bc 100 a 100 a 100 a 75 ab 75 ab 45 c 64.3 bc 58.8 c 58.5 c 63.6 bc 63.8 bc 70.3 a 64.8 b LSD P = 0.05 7 6.9 0.4 29.3 5.32 1Treatments were applied on August 1 (R2 growth stage), August 15 (R3-R4), and September 1 (R5). 2 Leaf greening was rated on a 0 to 3 scale with 3 representing a high level of green foliage being retained by the plant. Incidence of Asian soybean rust (ASR) was determined by examining five plants per plot. 4 Means followed by the same letter are not significantly different. 2005 SOYBEAN RESEARCH REPORT 13 SOYBEAN BREEDING BREEDING IMPROVED SOYBEAN CULTIVARS FOR ALABAMA D. B. Weaver The testing and development of new soybean (Glycine max L.) cultivars continues to be an ongoing project of the Alabama Agricultural Experiment Station. Since its inception, the project has resulted in the release of three cultivars (Stonewall, Carver, and Kuell) and two germplasm lines. More lines are in various stages of development. During 2005, experimental soybean lines were tested, in- cluding 30 Maturity Group (MG) VI lines and 80 MG VII lines tested at one location (Tallassee) with two repetitions in multi- ple-row plots. A total of 50 lines (20 MG VI, 20 MG VII, and 10 MG VIII) were tested in advanced trials at multiple locations in Alabama. The best-performing of these lines will be advanced to the next level of testing, the USDA Cooperative Uniform Tests. In the USDA tests, five of the best advanced lines were tested in the Preliminary VI test, four in the Preliminary VII test, and two in the Preliminary VIII test. Only one line, a MG VI line that ranked fifth overall in the 2004 Preliminary test, was advanced into the 2005 Uniform test. Several of these lines performed well in the 2005 Preliminary tests and will be advanced to the Uni- form Tests for 2006. The future populations will be developed with the objective of cultivar development. Additional Breeder seed of Kuell were produced in 2005 and should be available to Registered and Certified Seed producers in 2006. In a cooperative project with Clemson University, 38 dif- ferent populations of material were grown with a combination of the long-juvenile trait (lack of photoperiod response, or pho- toperiod insensitive) and Roundup-Ready technology. Soybean cultivars that do not begin reproductive growth in response to daylength (photoperiod insensitive) have the potential to expand the range of planting dates with no detrimental effects on yield. This trait would allow lines to be planted either very early to avoid late-summer drought or planted late, in a double-cropping system following small grains or maize. Experimental lines have produced well at both planting dates and outperformed check cultivars at these extreme planting dates. Another trait carried by this material is resistance to soybean cyst nematode and root-knot nematode. Single-plant selections were made and will continue to be evaluated for the next couple of years with the objective of developing a germplasm or cultivar with these two traits. Several years of yield tests with experimental long- juvenile lines have been conducted, and the long-juvenile trait is now being combined with Roundup Ready technology for com- mercial production. In cooperation with the USDA, 600 soybean accessions in MGs VI, VII, VIII, IX, and X were evaluated for reaction to the Asian soybean rust. These accessions were previously screened from the entire USDA germplasm collection, having shown some rust resistance potential in previous tests in an off-shore containment facility. Disease was monitored closely, but suffi- cient rust did not develop in time for meaningful results to be obtained. Also in cooperation with the USDA Uniform Cooperative Tests, 11 tests are being conducted in three locations (Tallassee, Belle Mina, and Fairhope), and more than 200 public breeding lines of Maturity Groups V, VI, VII, and VIII in both Prelimi- nary and Uniform Tests are being evaluated. This continues to be a major resource of genetic material as well as a great testing network for evaluation of new genotypes from all public breed- ing programs in the Southeast. These tests are conducted with support from the Alabama Agricultural Experiment Station and soybean checkoff funding. 2005SOYBEANRESEARCHREPORT 13 WtitIl \uni n oI I1I\: 4~I Li op. I i\ cUtlock. toic I slix woillill - Il~m F\ ci \ clti/c ii nI Iii iv inn Cviillllllv: 1 I ,13 Research Unit Identification 9 Al :1 .i A&'t C i E V S7ii Ijc1,1 Gcwc Slicr',r Li'I " F ' wcu t I -1'. zvi- q-. 'i ~ 'iv q' i tIiFJ ,-i t nroiiA I '.'iirrj.-,*," C'C - ITJF I ;I X- ft "I . I '1 A it ic '1, J , I- .111 'i iv -v.1 f 0 v-, Cl 1 'J t )r F~ t