4$ 'V 'V Al~tbtn~ ~m 'Itu~a. L~~ ~me;7. iXn lntcrnn Dire urn Un veffi'o J-xn, on FRUIT PPR Performance of 'Apache' Blackberry in South Alabama ................................................................. 3 A Promising New Surfactant for Thinning Peach Blossoms, 2001 ....................................................... 4 Results of Peach Blossom Treated with Tergitol.TM7N-6, 2002 .......................................................... 4 Refined Applications Rates for Tergitol TMN-6 for Blossom Removal of Peach....................................... 5 Extending the Time of Application for Chemically Thinning Peach....................................................... 6 Evaluation of Flint and Elite for Brown Rot Control on Peaches ......................................................... 7 Evaluation of Sulfur-Based Fungicides for Scab Control on Peaches.................................................... 8 Evaluation of Captan/Sulfur Tank Mixes for Peach Scab and Brown Rot Control on Peaches ........................ 9 Evaluation of Ziram 76 DF for Red Spot Control on Peaches, 2002.................................................... 10 Screening of New Insecticides for Control of Plum Curculio in Peaches .............................................. 10 Frequency of Warm Winters Increases Need for Rest-Breaking Compounds......................................... 12 Effects of the Rate of Nitrogen and Times of Pruning on Three Varieties of Peach in Central Alabama, 2001 ............................................................. . . ..... 0...*... ...00..0...13 Effects of the Rate of Nitrogen and Times of Pruning on Three Varieties of Peach in Central Alabama, 2002 .......................................................................................... 14 Efficacy of the Soil Fumigants Telone C-35 and Telone 11 on Growth and Survival of Peach Trees on a Replant Site....................................................................... 0........0. 16 Evaluation of Fungicide Spray Programs for Pecan Scab Control ...................................................... 17 Alternate Bearing in Satsumas ............................................................................................. 18 Satsuma Disease Survey of Alabama.................................................................................... 18 Evaluation of Fungicides for Botrytis Gray Mold Control on Strawberries, 2002 ...................................... 19 VEGETABLE PAPERS Evaluation of Fungicides for Control of Alternaria Leaf Spot of Cabbage.............................................. 21 Effect of Nitrogen Source on Quality and Yield of 'LaRouge' Irish Potato............................................ 21 Effect of Split Application of Desiccant and Vine Rolling on Quality and Yield of 'LaRouge' Irish Potato .............................................................. 22 Evaluation of Synthetic and Biological Fungicides for Control of Powdery Mildew on Pumpkin........... s.e... 23 Evaluation of 1PM Spray Programs for Control of Foliar Diseases of Pumpkin, 2001 ....... ....... 24 Comparison of TerraPy G and TerraPy B to Methyl Bromide as Growth Promoters for Tomato, 2001................................................................................................. 25 Evaluation of TerraPy G at Multiple Rates as a Growth Promotion for Tomato, North Alabama, 2001............................................................................................... 26 Evaluation of TerraPy G at Multiple Rates as a Growth Promotion for Tomato, SadMuti,201................................7 Robert Boozer Area Horticulturist Chilton Area Horticulture Station S.L. Burchett Graduate Research Assistant Department of Horticulture Jason Burkett Superintendent-Horticulture Unit E. V. Smith Research Center Arnold Caylor Superintendent North Alabama Horticulture Station Tony Dawkins Superintendent Sand Mountain Research and Extension Center William Dozier Professor Department of Horticulture Robert Ebel Co-editor Associate Professor Department of Horticulture Wheeler Foshee Extension Program Associate Dept. of Entomology and Plant Pathology Dan Horton Professor and Extension Entomologist University of Georgia Joseph Kemble Co-editor Associate Professor and Extension Horticulturist Department of Horticulture Joseph W. Kloepper Professor Department of Entomology and Plant Pathology Ron McDaniel Superintendent Gulf Coast Research and Extension Center Kathy McLean Associate Professor Dept. of Entomology and Plant Pathology John F. Murphy Associate Professor Dept. of Entomology and Plant Pathology Monte Nesbitt Area Horticulturist Gulf Coast Research and Extension Center Malcomb Pegues Assistant Superintendent Gulf Coast Research and Extension Center Jim A. Pitts Superintendent Chilton Area Horticulture Station M.S. Reddy Professor Alabama A&M University Jeff Sibley Associate Professor Department of Horticulture Clifford Sikora Edward Sikora Associate Professor and Extension Plant Pathologist Department of Horticulture Johnny Staples Raymond Thomas Graduate Research Assistant Department of Horticulture Edgar Vinson Research Assistant Department of Horticulture Bryan Wilkins Graduate Research Assistant Department of Horticulture Floyd M. Woods Associate Professor Department of Horticulture AUTHORS FRUIT PAPERS PERFORMANCE OF 'APACHE' BLACKBERRY IN SOUTH ALABAMA Monte Nesbitt, Ron McDaniel, and Malcomb Pegues Six cultivars of thornless blackberries are being evaluated 'A at the Gulf Coast Research and Extension Center in Fairhope, based or Alabama. Cultivars being studied include 'Apache', 'Arapaho', 'Apache 'Chester', 'Loch Ness', 'Navaho', and 'Triple Crown'. Details of 2001, it c the planting and results from the first harvest were described in have exc the 2000 Fruit and Vegetable Research Report. No data were than sor presented for 'Apache' in the 2000 report because it was planted sive tha in midsummer 1999 and did not bear fruit until 2001. taste is c 'Apache' was released in 1999 by the University ofArkan- and is be sas, as a large-fruiting, thornless cultivar with resistance to or- quicklye ange rust disease. Orange rust has been found on three plants of vantage. 'Navaho' in this trial, and one plant of 'Arapaho', but has not later tha been detected on 'Apache', 'Chester', 'Loch Ness', or 'Triple tinuing i Crown'. No double-blossom disease has been detected to date Th in the entire planting, but minor cane and leaf damage has been a problei caused by Cercospora and Colletotrichum fungi. was 850 YIELD OF SELECTED BLACKBERRY CULTIVARS Cultivar Fruit Berries Berries Pints Harvest (Ibs/plant) (no/Ib) (no/pt) (no/plant) period 2001 Apache 4.1 62 43 6 5/23-7/27 Arapaho 5.8 107 73 9 5/14-6/22 Navaho 6 103 74 8 5/21-7/11 2002 Apache 10 67 46 14 6/7-7/31 Arapaho 5.3 133 94 8 5/24-6/26 Navaho 3.7 107 80 5 5/30-7/31 1 Taste score 1-5, 5=best. Spache' was the best performing cultivar in 2001 and 2002, n yield and fruit size data (see table). Although yield of ' was slightly lower than 'Arapaho' and 'Navaho' in Slearly out-yielded either cultivar in 2002. Fruit of 'Apache' oellent eye appeal and size. While the size is still smaller ie thorny cultivars like 'Kiowa', it is much more impres- n the other thornless cultivars in this trial. Flavor and omparable to 'Navaho', 'Arapaho', and 'Triple Crown', tter than 'Chester' and 'Loch Ness'. 'Apache' fruit soften after harvest, and shelf-life is limited, which is one disad- 'Apache' has a harvest period similar to 'Navaho' and n 'Arapaho', beginning in late May or early June, con- into late July, and peaking in late June. iere was some concern that insufficient chilling might be m on the Gulf Coast. Chill hour accumulation at Fairhope in 2000-01 and 700 in 2001-02, but the high production of 'Apache' in 2002 would sug- gest that its chill hour require- ment is not higher than 700 Taste Maximum hours. While continued evaluation is planned, it ap- pears that 'Apache', S 3.1 11.2 'Arapaho', and perhaps 'Na- S 3.4 11.5 vaho', have some merit for the L 3 12.2 Gulf Coast. We see no poten- tial in 'Chester', 'Loch Ness', or 'Triple Crown' as cultivars 3.4 12.9 for South Alabama. 3.5 14 L 3.1 14 A PROMISING NEW SURFACTANT FOR THINNING PEACH BLOSSOMs, 2001 Bryan Wilkins, Robert Ebel, Jim A. Pitts, and Robert Boozer Peach trees are prolific fruit producers and set more fruit than the tree can support. Trees must be thinned to have fruit that are of acceptable size for market. Thinning is usually done by hand, which is very costly and time consuming. In the late 80s and early 90s researchers at Auburn Univer- sity worked with several different chemicals to try and find an effective bloom thinner. Surfactant WK proved to be very prom- ising. Researchers were able to determine that the active ingredi- ent was Tergitol TMN-6 or Tergitol TMN-10. In 2000, Tergitol TMN-6 and Tergitol TMN-10 were tested at two rates, 2% and 4%, and at two stages of bloom develop- ment, full bloom and petal fall. Both chemicals thinned effec- tively and had no adverse ef- fects on fruit quality. How- ever, the 4% rate at petal fall defoliated trees and was omit- ted in future tests. In 2001, only Tergitol TMN-6 was tested at varying rates. The study was ar- ranged as a randomized com- plete block design with seven treatments and five replica- tions in single tree plots with a buffer tree between each treatment tree and a buffer row between each treatment row. Trees were sprayed at 90% full bloom and at petal fall with concentrations of either 0%, 1%, 2%, or 3%. Bloom counts were taken before spraying and fruit counts were taken before hand thinning. All normal com- mercial practices were followed in regards to harvest. Time of application did not alter thinning but thinning early did increase fruit size at hand thinning (see table). Thin- ning with the chemical did not adversely affect fruit quality. The extent of thinning correlated with the rate of chemical applied. Fruit quality was not adversely affected by the chemical. Tergitol TMN-6 effectively thinned peach blossoms without adverse ef- fects on the tree or fruit. FLOWER REMOVAL AND FRUIT GROWTH OF PEACHES TREATED WITH TERGITOL TMN-6, 2001 Fruit -Fruit hand thinned- Total fruit Fruit set weight number harvested Yield weight (%) (g/fruit) (no/tree) (no/tree) (Ibs/tree) (g) Concentration (%) 0 46.0 a' 16.2 c 1416 a 713 a 186 127 d 1 37.0 b 18.8 b 686 b 689 ab 213 144 c 2 23.0 c 20.8 b 456 bc 490 bc 180 167 b 3 9.0 d 24.3 a 138 c 347 c 149 192 a Time of application Control 46 a 16 b 1416 a 713 a 84 127 b Full bloom 22 b 22 a 348 b 424 b 71 171 a Petal fall 24 b 21a 506 b 594 ab 93 165 a SMeans separation within columns by Duncan's Multiple Range Test p = 0.05; columns without letters are not significantly different. RESULTS OF PEACH BLOSSOM TREATED WITH TERGITOL TMN-6, 2002 Bryan Wilkins, Robert Ebel, Jim A. Pitts, and Robert Boozer This is a continuation of a study that was initiated in 2000 at the Chilton Research and Extension Center in Clanton, Ala- bama, to test the efficacy of two surfactants as peach blossom thinners. In 2002, the study was repeated using the same rates and application times as in 2001 [ 0% (control), 1%, 2%, and 3% con- centrations at full bloom and petal fall]. The experimental design consisted of five blocks with single trees treated within each block. There were buffer trees between treated trees within each row and buffer rows between treated rows to reduce contamina- tion by spray drift. Five limbs that were 12 to 18 inches long were tagged on each data tree and the number of flowers were counted and classified according to stage of development. The treatments were applied using an airblast sprayer. All normal commercial practices were followed after treatment. Data collected included pretreatment flower counts, pre-hand thinning fruit counts on data limbs to determine fruit set, and number of fruit hand thinned per tree. There were no significant differences in fruit weight at hand thinning and the number of fruit hand thinned per tree (see table). Amount of thinning did increase slightly with increasing chemical rate. There were no significant differences in time of application. There were no adverse effects on harvest or fruit quality at harvest by the chemical. These results do not reflect previous work with this chemical nor are there any apparent reasons for these differences from previous studies. ALABAMAAGRICULTURAL ExPERIMENT STATION4 2001-2002 FRUIT AND VEGETABLE RESEARCH REPORT 5 FLOWER REMOVAL AND FRUIT GROWTH OF 'FIREPRINCE' PEACH TREES TREATED WITH TERGITOL TMN-6, 2002 Fruit -Fruit hand thinned- Total fruit Fruit set weight number harvested Yield weight Concentration (%) (g/fruit) (no/tree) (no/tree) (lbs/tree) (g) 0 60.0 a' 12.8 356 548 163 143 1 61.0 a 12.9 368 698 187 135 2 54.0 a 14.2 337 590 161 145 3 40.0 b 14.0 328 689 255 167 Time of application Control 59 13 712 548 74 143 Full bloom 53 13 779 713 94 145 Petal fall 51 14 597 605 89 154 Means separation within columns by Duncan's Multiple Range Test p = 0.05; columns without letters are not significantly different. EFINED APLICATION RATES FOR TERGITOL TMN-6 FOR BLOSSOM REMOVAL OF PEACH Bryan Wilkins, Robert Ebel, Jim A. Pitts, and Robert Boozer In previous experiments, Tergitol TMN-6 was effective in thinning peach blossoms; however, the rates that have been tested-1%, 2%, 3%, and 4% by volume-have either not re- moved enough of the flowers, (1%), or have removed too many flowers, (2%, 3%, and 4%). The objective of this experiment was to determine the most effective rate of Tergitol TMN-6 on the thinning of peach. This study was initiated in the spring of 2002 at the Chilton Research and Extension Center in Clanton, Alabama. Three trees of 'Sunland' peach were sprayed at petal fall with rates of 0% (control), 1%, 1.5%, and 2% by volume Tergitol TMN-6 with an airblast sprayer. The center tree of the three was used as the data tree and an unsprayed buffer tree was between each plot with a buffer row between treated rows. Four limbs 1 to 2 feet in length were selected on each treatment tree and the total flower count and the stage of physiological development were determined prior to treatment. Before hand thinning, the number of fruit on each tagged limb was counted to determine fruit set. A drop cloth was placed under one half of the tree at time of hand thinning and the fruit collected to determine the number of fruit hand thinned per tree. Fruit were harvested according to normal commercial practices. There were no significant differences in fruit set or fruit weight at hand thinning; however, there was a trend towards decreased fruit number that required hand thinning with increased rate of Tergitol (see table). There were no adverse effects on harvest or fruit quality. FLOWER REMOVAL AND FRUIT GROWTH OF 'SUNLAND' PEACH TREES TREATED WITH TERGITOL TMN-6, 2002 Fruit -Fruit hand thinned- Total fruit Fruit set weight number harvested Yield weight (%) (g/fruit) (no/tree) (no/tree) (lbs/tree) (g) Concentration (%) 0.0 491 13.0 504 282 79 129 c 1.0 52 12.6 472 249 79 146 a 1.5 46 13.5 442 290 82 132 b 2.0 41 13.8 346 204 63 142 ab 1 Means separation within columns by Duncan's Multiple Range Test p = 0.05; columns without letters are not significantly different. 6 ALABAMA AGRICULTURAL EXPERIMENT STATION ErENDING THE TIME OF APPLICATION FOR CHEMIALLY THINNING PEACH Bryan Wilkins, Robert Ebel, Jim A. Pitts, and Robert Boozer Peach trees set more fruit than the tree is able to support. Therefore, to have fruit that is of acceptable market size, up to 95% of the fruit must be removed from the tree. This is a very costly and time consuming process that is done primarily by hand. Currently no acceptable chemical thinners are available. The chemicals that are available are not consistent from year to year and the results from their applications cannot be seen for several weeks. These chemicals must also be applied within a very narrow application window to be effective. In a previous study conducted at the Chilton Research and Extension Center in Clanton, Alabama, Tergitol TMN-6 was shown to be an effec- tive blossom thinner of peach that could be applied at full bloom and petal fall and still provide adequate thinning. In the spring of 2002, a study was initiated with the objec- tive of determining the effect of Tergitol TMN-6 after petal fall on the blossom thinning of peach. Tergitol TMN-6 was applied to 'Harvester' peach at four dif- ferent stages of blossom de- velopment (full bloom, petal fall, shuck split, and shuck off) at rates of 0% (control), 1%, 2%, and 3% by volume with an airblast sprayer. The experi- mental plot was a randomized complete block design with single treatment trees with a buffer tree between treatment trees and a buffer row between treatment rows to minimize drift. Four limbs 1 to 2 feet in length were selected on each treatment tree and the total flower count and the stage of physiological development were determined prior to treat- ment. Before hand thinning, the number of fruit on each tagged limb were counted to determine fruit set. A drop cloth was placed under one half of the tree at hand thinning and the fruit collected to determine the number of fruit hand thinned per tree. Fruit were harvested according to normal commercial practices. Time of application had no affect on the amount of thin- ning, but thinning early did increase fruit size at hand thinning (see table). The amount of thinning increased with the increasing chemical concentration. Some damage occurred to fruit when the chemical was applied after petal fall, with the two highest rates having the most damaged fruit. Also the foliage was severely burned, and at the two highest rates the trees at shuck split were heavily defoliated. Due to concerns about phytotoxicity, the shuck off treatment was not applied. The highest rates had the fewest number of fruit and the lowest yield. There were no ad- verse effects on fruit quality. FLOWER REMOVAL AND FRUIT GROWTH OF 'HARVESTER' PEACH TREES TREATED WITH TERGITOL TMN-6, 2002 Fruit -Fruit hand thinned- Scarred Total fruit Fruit set weight number fruit harvested Yield weight (%) (g/fruit) (no/tree) (%) (no/tree) (lbs/tree) (g) Concentration (%) 0 39 a' 11.6 3548 a 0 c 705 124 90 1 41 a 14.6 2251 b 2 bc 523 131 120 2 36 ab 15.5 1620 bc 12 ab 425 111 126 3 29 b 15.8 1472 c 16 a 411 108 124 Time of application Control 39 11.6 b 3548 0 b 705 124 90 Full bloom 32 16.3 a 1787 0 b 453 120 127 Petal fall 39 15.3 a 1977 1 b 471 121 125 Shuck split 35 14.3 a 1579 28 a 434 109 119 1 Means separation within columns by Duncan's Multiple Range Test p = 0.05; columns without letters are not significantly different. 2001-2002 FRUIT AND VEGETABLE RESEARCH REPORT 7 EVALUATION OF FLINT AND ELITE FOR BROWN RoT CONTROL ON PEACH Edward Sikora, Jim A. Pitts, Robert Boozer, and Clifford Sikora This test was conducted to evaluate fungicides targeted for use in the preharvest period for control of brown rot. The fungicides Elite and Flint were evaluated at various rates indi- vidually, and as tank-mix partners. Results showed no differ- ences in brown rot control among the treatments though all were more effective than the unsprayed control treatment. These fun- gicides did not appear to have a beneficial effect in controlling Rhizopus rot, another postharvest disease of peaches. The experiment was conducted on a block of 'Harvester' peach trees at the Chilton Research and Extension Center in Clanton, Alabama. The experiment consisted of eight treatments, replicated five times, in a randomized complete block design. Treatments consisted of preharvest spray programs that com- pared Elite, Flint, or tank mixes of the two products in various combinations. All trees were maintained during the bloom and cover period following commercial production practices typical for the area. All trees were sprayed with Captan 50 WP at 5 pounds per acre on March 13, and with Captan 50 WP 3 pounds per acre plus sulfur 80% at 5.5 pounds per acre on March 22, April 2, April 6, April 20, May 4, and May 14. Preharvest treatments were ap- plied on June 4, June 7, June 11, and June 13. The station super- intendent decided on the closely spaced application schedule, which he felt was necessary as he suspected the fungicides did not have a sufficient time to dry on the fruit before heavy rains prevailed shortly after the fungicides were applied. TABLE 1. PERCENT BROWN ROT AND RHIzoPus ROT No symptoms of brown rot or any signs of phytotoxicity were observed on trees one day prior to the first harvest date. A total of 40 fruit per plot was sub-sampled at harvest (June 14) and stored in trays at approximately 75 0 F for eight days. Percent fruit with brown rot and Rhizopus rot was determined four and eight days after the first harvest date. A second harvest was conducted on June 18, five days after the last fungicide application. A total of 20 fruit per plot were sub-sampled on the second harvest date and stored in trays at approximately 75 0 F for four days. Percent of fruit with brown rot and Rhizopus rot was determined four days after the second harvest (June 22), which was nine days after the final fungicide application. Yield in pounds of marketable fruit was determined. All the fungicide treatments had significantly less brown rot than the unsprayed control at both four and eight days postharvest (with the last fungicide application one day prior to harvest) (Table 1). There were no significant differences in Rhizo- pus rot among the treatments at either rating period. All the fungicide treatments had significantly less brown rot than the unsprayed control four days postharvest (with the last fungicide application five days prior to harvest) (Table 2). There were no significant differences in Rhizopus rot among the treatments. The Flint 50 WG treatment at 3 ounces per acre had the highest yield, significantly higher than the two lower rates of Elite treatments. In general, yields were highest in the Flint- FOUR AND EIGHT DAYS only treatments followed by the POSTHARVEST' -Four days postharvest- -Eight days postharvest- Treatment Rate/acre Brown rot Rhizopus Brown rot Rhizopus (%) (%) (%) (%) Unsprayed control - 5.0 a 20.5 a 8.5 a ELITE 45 WG 5 oz 0.0 b 0.5 a 0.0 b 9.5 a w/t Induce 0.06% ELITE 45 WG 6 oz 0.0 b 0.5 a 0.0 b 10.0 a w/t Induce 0.06% ELITE 45 WG 8 oz 0.0 b 1.0 a 0.0 b 9.5 a w/t Induce 0.06% FLINT 50 WG 2 oz 0.0 b 0.6 a 0.0 b 8.7 a FLINT 50 WG 3 oz 1.5 b 1.5 a 1.5 b 9.5 a ELITE 45 WG 2.9 oz 0.0 b 0.0 a 0.0 b 3.0 a + FLINT 50 WG 2.6 oz ELITE 45 WG 3.5 oz 0.5 b 1.5 a 0.0 b 11.0 a + FLINT 50 WG 3.1 oz 1The last fungicide application was one day prior to harvest, 2001. 2 Numbers followed by the same letter are not significantly different. Flint/Elite tank-mix treatments and the Elite-only treatments. 8 ALABAMA AGRICULTURAL EXPERIMENT STATION TABLE 2. PERCENT BROWN ROT AND RHIZOPUS ROT FOUR DAYS POSTHARVEST -Four days postharvest- Treatment Rate/acre Brown rot Rhizopus Yield (%) (%) (lbs/plot) Unsprayed control - 22.0 a 2 8.0 a 141.4 ab ELITE 45 WG 5 oz 0.0 b 3.6 ab 129.5 b w/t Induce 0.06% ELITE 45 WG 6 oz 0.0 b 5.0 ab 119.2 b w/t Induce 0.06% ELITE 45 WG 8 oz 0.0 b 0.0 b 140.1 ab w/t Induce 0.06% FLINT 50 WG 2 oz 0.0 b 2.2 ab 168.7 ab FLINT 50 WG 3 oz 0.0 b 5.7 ab 205.1 a ELITE 45 WG 2.9 oz 0.0 b 1.3 ab 162.0 ab + FLINT 50 WG 2.6 oz ELITE 45 WG 3.5 oz 0.0 b 2.5 ab 181.5 ab + FLINT 50 WG 3.1 oz 1 The last fungicide application was five days prior to harvest, 2001. 2 Numbers followed the same letter are not significantly different. EVALUATION OF SULFUR-BASED FUNGICIDES FOR SCAB CONTROL oN PEACHES Edward Sikora and Jim A. Pitts Peach scab is a common fungal disease of peaches in Ala- bama resulting in spotted and scarred fruit and reduced fruit quality. This test was conducted to evaluate various cover spray programs using Microthiol Disperss, a sulfur-based fungicide. These programs were compared to the Alabama peach industry control on peaches. Results from this study indicate that Microthiol Disperss at rates from 6 to 10 pounds per acre and in a tank-mix or rotational program with Captan 50 WP controlled scab as well as Wettable sulfur at 9 pounds per acre. The Microthiol Disperss/Captan tank-mix treatment and the Microthiol Disperss/Captan rotation treatments also performed as well as Captan 50 WP 5 pounds per acre in controlling scab. This experiment was conducted at the Chilton Research and Extension Center in Clanton, Alabama, in 2002. The test con- sisted of seven treatments replicated five times in a randomized complete block design. All treatments including the control re- ceived two Bravo Weatherstik applications at petal fall and shuck split and preharvest applications of Orbit at standard rates. There EFFECT OF FUNGICIDE SPRAY PROGRAMS ON SCAB INCIDENCE, CLANTON, 2002 Tratment Treatment rate per acre' Unsprayed control Microthiol Disperss 10 Ib Microthiol Disperss 6 lb Microthiol Disperss 3 lb + Caotan 50 WP 3.0 Ib Fruit with scab Marketable fruit (%) (%) 37.5 3.5 5.0 0.5 81.5 98.5 98.5 100.0 Captan 50 WP 5.0 lb 0.5 100.0 rotated with Microthiol Disperss 2 Wettable sulfur 90 WP 9 lb 4.5 100.0 Captan 50 WP 5 Ib 0.5 99.5 1 Each treatment received eight cover sprays. 2 Captan was used in cover sprays 1, 3, 5, and 7 and Microthiol Disperss was used in sprays 2, 4, 6, and 8. 2001-2002 FRUIT AND VEGETABLE RESEARCH REPORT were a total of eight cover sprays applied for each treatment. Forty fruit were collected from each two-tree replication at har- vest and evaluated for incidence and marketability (disease se- verity) of scab. Fruit were stored at 75 0 F for seven days then rated for the postharvest fungal diseases brown rot and Rhizo- pus rot. All fungicide treatments performed significantly better than the unsprayed control (see table). Scab incidence ranged from 9 0.5 to 5.0% among the fungicide treatments. Treatments that used Captan 50 WP had slightly lower levels of scab incidence than programs that used Microthiol Disperss or Wettable sulfur alone full season. There were no significant differences among the fungicide programs in fruit marketability, and all produced sig- nificantly more marketable fruit than the unsprayed control. There were no differences in brown rot or Rhizopus rot control among the treatments, including the unsprayed control (data not shown). EVALUATION OF CAPTAN/SULFUR TANK XES FOR PEACH SCAB AND BROWN SPOT CONTROL ON PEACHES Edward Sikora, Jim A. Pitts, Robert Boozer, and Clifford Sikora Peach producers in Alabama commonly use sulfur as part of their disease management program. To improve its effective- ness, and to keep costs relatively low, some growers tank-mix sulfur with the fungicide Captan for spraying during the cover period. How effective this program is in controlling peach dis- eases and the relative ratio of sulfur to Captan needed for control are still not clear. This reports outlines the results of the third year of a three-year study comparing two sulfur/Captan tank-mix programs with the standard, full season cover spray programs of sulfur or Captan alone. The experiment was conducted at the Chilton Research and Extension Center near Clanton, Alabama, on the cultivar 'Alred Alberta'. Treatments consisted of cover spray programs of (1) unsprayed control, (2) Captan 50 WP at 5 pounds per acre, (3) Sulfur 80% at 9 pounds per acre, (4) Captan 50 WP 3 pounds per acre plus Sulfur 80% at 5.5 pounds per acre, and (5) Captan 50 WP 2 pounds per acre plus Sulfur 80% at 3.5 pounds per acre. All the fungicide programs performed significantly better than the unsprayed control in terms of scab incidence and mar- ketability of fruit (see table). The sulfur-only program had a sig- nificantly higher level of scab incidence compared to the Captan and the Captan/sulfur tank-mix programs. The sulfur-only pro- gram also produced significantly less marketable fruit than the Captan program and the higher rate program of the Captan/sulfur tank mix. Brown rot was a significant problem based on the high level of disease incidence on the unsprayed control. There was no significant difference in brown rot incidence among the fungi- cide programs; however disease incidence was highest in the sulfur-only program and progressively less with higher rates of Captan. There were no significant differences in Rhizopus rot among the treatments, including the unsprayed control. Results from this trial are similar to what was observed in 1999. Spray programs consisting of Captan alone at 5 pounds per acre or Captan 3 pounds per acre plus Sulfur at 5.5 pounds per EVALUATION OF CAPTAN/SULFUR TANK MIXES FOR PEACH SCAB AND BROWN ROT CONTROL ON PEACHES, 200.1 Fungicide cover Fruit Marketable Brown Rhizopus spray program' with scab fruit rot rot Unsprayed control 92.7 a 2 16.2 c 65.6 a 18.4 a Captan 50 WP 5 Ib/ac 5.7 c 96.8 a 8.8 b 23.9 a Sulfur 80% 9 Ib/ac 31.3 b 84.1 b 19.2 b 28.6 a Captan 50 WP 3 lb/ac + 5.0 c 98.0 a 11.6 b 264 a Sulfur 80% 5.5 lb/ac Captan 50 WP 2 Ib/ac + 17.5 c 91.2 ab 16.5 b 25.7 a Sulfur 80% 3.5 lb/ac Bravo Ultrex was applied atshuck split and petal-fall and two Orbit sprays were applied at seven and one day before harvest for all treatments except the control. A total of 40 fruit were picked from the center two trees of each treatment/replication. Percent of fruit with scab and percent marketable fruit were determined at harvest. Incidence of brown rot and Rhizopus rot was deter- mined seven days after harvest. 2Numbers followed by the same letter are not significantly different from one another. acre had fewer fruit with scab lesions and higher levels of marketable fruit compared to the sulfur-only program. In the three-year study, the tank- mix programs have usually performed as well as the Cap- tan-only program, though the lower rate of the Captan/sul- fur tank-mix program is less effective in high scab pres- sure situations. The Captan/ sulfur tank-mix programs and the sulfur-only program may also suffer from heavier losses from brown rot in high brown rot-pressure years, though this needs to be investigated further. ALABAMA AGRICULTURAL EXPERIMENT STATION E VALUATION OF ZiRm 76 DF FoR RED Sor CONTROL ON PEACHEs, 2002 Edward Sikora, Jim A. Pitts, Robert Boozer, and Wheeler Foshee Red spot is a common problem in Chilton County on light- skinned peaches. The cause of the disease is still unclear. One report suggests red spot is caused by an Alternaria-type fungus, but this has not been confirmed in Alabama. Ziram 76 DF has been observed to suppress red spot in South Carolina. This trial was conducted to determine if Ziram would reduce red spot se- verity in Alabama. Results indicate that the 4- and 5-pound rates of Ziram reduced red spot severity on peach fruit. The experiment was conducted at the Chilton Research and Extension Center in Clanton, Alabama, in 2002. The test con- sisted of four treatments replicated four times in a randomized complete block design. All treatments including the control re- ceived two Bravo Weatherstik applications at petal fall and shuck split and one preharvest application of Orbit at standard rates. There were a total of 14 Ziram 76 DF applications applied weekly starting on April 18 and ending on July 19. Twenty fruit were collected from each replication at harvest and the number of red spots per fruit was determined. All Ziram 76 DF treatments performed better than the unsprayed control in reducing severity of red spot on peach fruit (see table). None of the treatments provided 100% control. There were no significant difference between the 4- and 5-pound rates of Ziram. The success of Ziram 76 DF in reducing red spot does suggest that the disease is caused by a fungal pathogen. The recommendation in South Carolina calls for three to four applications of Ziram 76 DF at a rate of 4 pounds per acre beginning seven to 10 days after shuck split and reapplication every 14 days with a total of three to four applications, followed by standard cover spray program. In this trial, Ziram was applied weekly with a total of 14 applications, which may not be eco- nomical. In 2003, the experiment will be repeated and the recom- mendations suggested in South Carolina followed. EFFECT OF ZIRAM 76 DF ON RED SPOT ON PEACHES, 2002 Treatment Percent of fruit with red spots (ranging from 0 to 15<) rate/acre' 0 1-5 6-15 15< Unsprayed control 46.2 16.2 12.5 25.0 Ziram 76 DF 3 lb 78.7 3.7 11.2 6.2 Ziram 76 DF 4 Ib 92.5 1.2 3.7 2.5 Ziram 76 DF 5 Ib 91.2 1.2 3.7 3.7 SEach treatment received 14 Ziram 76 DF applications. SCREENING OF NEW INSECTICIDES FOR CONTROL OF PLUM CURCULIO IN PEACHES Wheeler Foshee, Robert Boozer, Dan Horton, and Johnny Staples The plum curculio (Conotrachelus nenuphur) is the most destructive pest of peaches grown in the Southeast. Control of plum cucurlio in the past was very effective with organophos- phates, particularly the use of encapsulated methyl parathion until it was canceled by EPA in 1999. In 2001, use of azinphos- methyl (Guthion), another organophosphate, was restricted in the number of applications and this insecticide will be canceled by the end of 2005. The use of new chemistries as possible alternatives to or- ganophosphates for plum cucurlio control needs evaluating. With many new insecticides available, a faster screening method is needed to select candidates for field testing. The objective of our study was to develop a screening method and to test some newer insecticides for the control of plum curculio in peaches. During the spring of 2002, adult plum cucurlio were ob- tained from traps within orchards in Chilton County, Alabama. Insecticides evaluated (Table 1) were applied at an equivalent rate of 50 gallons per acre using a Burkhard hand-applicator at a rate of 0.5 FI to each plum cucurlio adult. All spray mixtures re- ceived a 0.5 v/v addition of Destiny? surfactant, a modified seed- oil. Mortality and insecticide intoxication data were taken at 24, 48, 72, and 96 hours postapplication. Preliminary results indicate that the organophosphate malathion and the pyrethroid bifenthrin (Capture) performed bet- ter, in most cases, than all other treatments. At 24 hours postapplication, the only 100% mortality/intoxicated state was observed in bifenthrin (2x rate), malathion, and malathion (2x rate) treatments. These were also the only treatments at the 48- 10 2001-2002 FRUIT AND VEGETABLE RESEARCH REPORT 11 hour post-application point that reached 100% mortality/intoxi- cation. Several other treatments did improve at the 48-hour post- application evaluation (Table 2). At 72 hours postapplication, bifenthrin (2x rate), malathion, and malathion (2x rate) were still at 100% mortality/intoxication. The treatments similar to these were fipronil, fipronil (2x rate), bifenthrin, and thiamethoxam (2x rate) (Table 2). The final assessment was at 96 hours and natural mortality may have become a factor. The following treatments gave 100% mortality/intoxication: bifenthrin (2x rate), malathion, malathion (2x rate), and fipronil (2x rate). These had higher mortality/intoxi- cation states than thiacloprid (2x rate) (33%) and the untreated 2x check (17%) (Table 2). The high level of plum curculio mortalityby malathion and bifenthrin demonstrated that the older chemistries, at this point, still have superior efficacy. It is worth noting that among other materials tested, only fipronil at the 2x rate gave 100% mortality/ intoxication after 96 hours. Fipronil is known to be slow-acting, so these results are noteworthy. At the 72-hour postapplication point, the only new chemistries to give 83% control were the lx and 2x rates offipronil. Thiamethoxam and thiacloprid failed, giv- ing only 33% control. Results are preliminary and further testing of these new chemistries to validate the results is needed before field tested can be suggested. TABLE 1. INSECTICIDES EVALUATED, FORMULATION, RATES, AND AMOUNTS MIXED FOR A 20ML MIX Rate Amount of product Treatment Formulation (ib ai/ac) per 20 ml mix 2x rates Thiamethoxam(Actara?) 25wg 0.078 14.95 mg 29.9 mg Thiacloprid (Calypso?) 4EC 0.2568 25.68 ml 51.3 ml Fipronil (Termidor@) 0.8 SC 0.25 125 ml 250 ml Bifenthrin (Capture@) 2EC 0.10 20 ml 40 ml Malathion 50% EC 0.5 400 ml 800 ml TABLE 2. RESULTS OF EFFICACY OF TESTED INSECTICIDES FOR CONTROL OF PLUM CURCULIO Percent intoxicated and mortality Treatment 24 hours 48 hours 72 hours 96 hours Thiamethoxam (Actara@) 33 cd 33 bc 33 bc 50 abc Thiamethoxam - 2x (Actara@) 50 bc 50 abc 67 ab 67 abc Bifenthrin (Capture@) 50 bc 67 ab 83 a 83 ab Bifenthrin - 2x (Capture@) 100 a 100 a 100 a 100 a Thiacloprid (Calypso?) 17 cd 33 bc 33 bc 50 abc Thiacloprid - 2x (Calypso?) 33 cd 33 bc 33 bc 33 bc Malathion 100 a 100 a 100 a 100 a Malathion - 2x 100 a 100 a 100 a 100 a Fipronil (Termidor?) 50 bc 67 ab 83 a 83 ab Fipronil - 2x (Termidor?) 83 ab 83 ab 83 a 100 a Untreated 50 bc 50 abc 17 c 50 abc Untreated - 2x 0 d 0 c 0 c 17 c Waller-Duncan K-ration T test. Means within columns followed by the same letter are not signifi- cant, P<0.05. 2001-2002 FRUIT AND VEGETABLERESEARCHREPORT 11 12 ALABAMA AGRICULTURAL EXPERIMENT STATION FREQUNCY OF WARM WINTERS INC REASES NEED FOR REST-BREAKING COMPOUNDS Robert Boozer and Jim A. Pitts One of the major concerns for peach producers in Central Alabama is the occurrence of early spring freeze damage to peaches. In addition, recent winter weather patterns have been warmer than normal resulting in insufficient chill hours. Chilling is necessary for normal rest breaking of fruit and leaf buds. One chill hour is one hour at or below 45 0 F and total hours are re- corded from October 1 through February 15. During five of the past eight years, Central Alabama has not received sufficient chill hours to completely satisfy the bud rest of 850 chill hour and higher peach varieties. Some of these warmer winters have af- fected leaf buds much more than fruit buds. Natural chilling is more desirable, but rest- breaking chemicals could be beneficial where chill hours might need to be supplemented. Producers would like to be able to decide as late as pos- sible before committing to applying rest-breaking chemicals. One of the major reasons is the high cost of the only currently labeled product, Dormex (hydrogen cyanamide). Other reasons are con- cerns for breaking rest too early and trying to utilize as much natural chilling as possible. Earlier work with Dormex indicated that effective applications were made when 72% of natural chill- ing for a variety had occurred. If chill hour accumulation picked up during February, would waiting until late February or early March before making an application work? This study was con- ducted to evaluate early and late applications of rest-breaking compounds. Dormex and Dropp (thidiazuron) are two compounds that have been reported and tested for the ability to stimulate buds into their growth phase. The time of application and rates with these compounds and the effect on fruit number, size, and shape are important questions that are still being investigated. Of the two compounds, only Dormex has a label at this time. In 2002 a study was conducted at the Chilton Research and Extension Center, Clanton, Alabama, in a block of'Cresthaven' peaches, 950 chill hour variety. A total of six treatments at two different application dates were evaluated on fruit bud develop- ment, fruit size, shape, and total yield. Treatments consisted of Dormex at 1.0%, 2.0%, and 4.0% plus nonionic surfactant at 0.25% applied on January 28 and March 7 (early pink bud stage). Dropp was applied at 200 parts per million and at 300 parts per million plus 2% Dormant Oil on the same dates. As a standard, one treatment consisted of an untreated control. Applications were made with an air-blast sprayer using 130 gallons spray volume per acre. On January 28 the total number of chill hours occurring since October 1,2001 was 706. Slightly over 74% of the chilling requirement had been satisfied for 'Cresthaven' at the time of the first application. Buds were in very early swell stage and con- tained light green pollen grains as discerned at 10x magnification when dissected. On March 7, when buds were at the early pink stage, the second application of each treatment was made. Chill hours totaled 923 by February 15 and 1129 by March 7, 2002. Fruit bud losses within the 'Cresthaven' block appeared to be related to weather as well as application ofDormex and Dropp. The highest rate of Dormex thinned buds, but bud survival was not statistically lower than the untreated control. Dropp seemed to provide some level of freeze protection. Fruit bud develop- ment was advanced by the use of Dormex at the first application date, but not the later application. The amount of advancement increased over time and was most significant at shuck-split. It seems the use of Dormex reduces the number of heat units needed to reach a certain fruit bud developmental stage (Figure 1). Dropp did not significantly advance fruit buds on either application date. Visible differences in leaf bud advancement were noted for Dropp with even more leaf bud advancement noticed for Dormex, after the first application. Fruit were harvested four times from July 15 through July 26. Total number of fruit and size of fruit were not significantly different among treatments. Adverse weather during the early growth phase of fruit was thought to have contributed to poor sizing. Size problems were also a problem in lower chill hour peach varieties near this location. Quality ratings on fruit shape were improved with Dormex and Dropp (Figure 2). Individual ratings for appearance [round (2), oval (4), tip (6), point (8), and suture (10)] were combined to provide an overall rating. The higher the number the less desir- able the characteristics. Fruit quality resulting from the first ap- plication was improved by 2% and 4% Dormex, and 200 parts per million Dropp over the untreated control. There were no statisti- Figure 1. Fruit Bud Development as Affected by Dormex and Dropp, 28 January Application 70- 11 M 1%Dr mx 0 2%Drmx I 4%Drmx 1I 200Dropp DAT = days after treatment ALABAMAAGRICULTURAL ExPERIMENT STATION12 2001-2002 FRUIT AND VEGETABLE RESEARCH REPORT cal differences in fruit quality due to the second application treat- ment. Dormex exceeds Dropp as a compound for breaking rest in fruit buds of peach. The only potential benefit from Dropp that might justify further work would be the potential increase in fruit bud hardiness during late winter and early spring freezes and potential benefit to fruit shape. Time of application was shown to be important for the use of Dormex. Applications made as late as early pink are not effective in advancing fruit buds. Dormex seems to do a good job in compensating chill hours and potentially reducing heat unit requirements, but will not compensate for poor growing conditions during early fruit development. Visible impact on leaf growth was consistent with other studies using Dormex. The physiological condition of the tree should be im- proved by a more normal transition out of the rest stage into growth stage and would likely benefit future productivity. 13 Figure 2. Fruit Quality Rating 28 January Application 18 S17.5 17- 16. 5- S 16 a s - 15- 14.5 ab abc bcd d Overall Fruit Shape O UTC E 1%Drmx E 2%Drmx O 4%Drmx El 200Dropp 0 300Dropp Higher value, more shape problems, max rating-22, min rating=8, LSD P=0.05 EFFECTS OF THE RATE OF NITROGEN AND TIMES OF PRUNING ON THREE VARIETIES OF PEACH IN CENTRAL ALABAMA, 2001 Bryan Wilkins, Robert Ebel, Robert Boozer, and Jim A. Pitts This is a continuation of a study initiated in 2000 at the Chilton Research and Extension Center in Clanton, Alabama. The objective of the study was to determine an optimal nitrogen rate and pruning regime for peach. The study is being conducted on three varieties of peach with different ripening dates: 'Surecrop' (early), 'Contender' (mid), and 'Encore' (late). During the grow- ing season, trees were pruned once (winter), twice (winter and shortly before harvest), or three times (right before hand thin- ning, two to three weeks before harvest, and after harvest). Ni- trogen was applied at 30, 60, and 90 pounds per acre per year. The experiment was a blocked (five blocks), split-plot design with nitrogen application as the main plot and pruning treatment as the split plot. Data collected included weights of all prunings, trunk cross sectional area, yield, fruit color, firmness, soluble solids, and average fruit weight. No fruit were produced in 2001. There were no significant differences for 'Surecrop' (see table) in trunk cross sectional area for the nitrogen treatment or pruning method. Pruning weights by pruning method were different, with the trees that were pruned twice having the least weight. Also photosynthesis and stomatal conductance by method of pruning were different, with the trees that were pruned three times a year having the highest rates. There were no differences in trunk cross sectional area for 'Contender' with respect to amount of N applied and pruning method. Pruning weights with respect to N rate and pruning method were significantly different. Also photosynthesis and stomatal conductance by method ofpruning were different, with the trees that were pruned three times a year having the highest rates. There were no differences in trunk cross sectional area for 'Encore' with respect to N rate or pruning method. Pruning weights by pruning method were different with the trees that were pruned two times having the highest weights. Also photosynthesis and stomatal conductance by method of pruning were significantly different with the trees that were pruned three times a year hav- ing the highest rates. 14 ALABAMA AGRICULTURAL EXPERIMENT STATION INFLUENCE OF NITROGEN RATE AND PRUNING METHOD ON PEACH (PRUNUS PERSICA (L.) BATSCH) PERFORMANCE IN 2001 Trunk cross- Pruning Photo- Stomatal sectional area weight synthesis conductance (cm 2 /tree) (kg/tree) (Pmol C0 2 / 2 /s 1 ) (mol H20 m-2S ) 'Surecrop' Nitrogen rate (lbs/acre) 30 155 1 9.1 15.7 0.190 60 161 10.7 15.6 0.195 90 139 8.8 15.7 0.195 Pruning (no. times/year) 1 169 12.7 a 14.1 c 0.159 c 2 143 6.2 b 15.5 b 0.182 b 3 143 10.8 a 17.4 a 0.239 a 'Contender' Nitrogen rate (Ibs/ac) 30 151 10.6 a 14.9 0.198 60 139 10.1 a 14.5 0.185 90 143 8.3 b 14.7 0.184 Pruning (no. times/year) 1 142 8.7 b 13.6 c 0.154 c 2 147 12.4 a 14.5 b 0.183 b 3 144 8.2 b 16.1 a 0.230 a 'Encore' Nitrogen rate (lbs/ac) 30 150 14.8 11.3 0.149 60 154 15.5 11.6 0.154 90 141 14.4 11.1 0.145 Pruning (no. times/year) 1 148 9.2 b 10.7 b 0.128 c 2 154 21.9 a 11.0 b 0.150 b 3 143 12.1 b 12.2 a 0.170 a Mean separation within columns by Duncan's Multiple Range Test p = 0.05, columns without letters were not significantly different. EFFECTS OF THE RATE OF NITROGEN AND TIMES OF PRUNING ON TRME VARIETIES OF PEACH IN CENTRAL ALABAMA, 2002 Bryan Wilkins, Robert Ebel, Robert Boozer, and Jim A. Pitts This is a continuation of a study initiated in 2000 at the Chilton Research and Extension Center in Clanton, Alabama. The objective of the study was to determine an optimal nitrogen rate and pruning regime for peach. The study is being conducted on three varieties of peach with different ripening dates: 'Surecrop' (early), 'Contender' (mid), and 'Encore' (late). During the grow- ing season,. trees were pruned once (winter), twice (winter and shortly before harvest), or three times (right before hand thin- ning, two to three weeks before harvest, and after harvest). Ni- trogen was applied at 30, 60, and 90 pounds per acre per year. The experiment was a blocked (five blocks), split-plot design with nitrogen application as the main plot and pruning treatment as the split plot. Data collected included weights of all prunings, trunk cross sectional area, yield, fruit color, firmness, soluble solids, and average fruit weight. This report summarizes the results of the 2002 study. Prun- ing weights for 'Surecrop' were significantly different with the trees that were pruned three times per year having the smallest 14 ALABAMA AGRICULTURAL ExPERIMENT STATION 2001-2002 FRUIT AND VEGETABLE RESEARCH REPORT weights (see table). There were significant differences in photo- synthesis and stomatal conductance by pruning method with the trees that were pruned one time having the lowest rates. Method of pruning made a significant difference on the number of fruit harvested and the total yield per tree. There were no significant differences in fruit weight, red blush, or soluble sol- ids. Trees that had the lowest N rate and were pruned three times had firmer fruit. For 'Contender', pruning weight by pruning method was significantly different with trees being pruned one time having the lowest weights. There were significant differences in photo- synthesis and stomatal conductance by pruning method with the trees that were pruned one time having the lowest rates. Total number of fruit harvested, total yield, soluble solids, or red blush were not affected by N or pruning treatments. There was a sig- nificant difference in fruit weight and firmness by pruning method with trees that were pruned one time having the lowest weights and being the least firm. Pruning weights for 'Encore' were significantly affected by pruning method with trees that were pruned one time having the highest weights. There were significant differences in photo- synthesis and stomatal conductance by pruning method with the trees that were pruned one time having the lowest rates. There were significant differences in total number of fruit per tree with trees being pruned one time having the most fruit. There were no significant differences in total yield, fruit weight, soluble solids, or red blush. Fruit firmness was affected by pruning with fruit from trees that were pruned three times being the most firm. At this time except for a few incidences, N rate has not had an effect on any of the parameters for the three varieties in this study. INFLUENCE OF NITROGEN RATE AND PRUNING METHOD ON PEACH (PRUNUS PERSICA (.) BATSCH) PERFORMANCE IN 2002 Pruning Photo- Stomatal Total Avg. fruit Fruit weight synthesis conductance fruit/tree Yield weight firmness (kg/tree) (gmol C0 2 /m 2 /s) (mol H 2 0 m- 2 s-1) (no) (kg/tree) (g/fruit) (Ibs) 'Surecrop' Nitrogen rate (lbs/acre) 30 12.4 13.0 0.236 349 39.7 164 7.7 a 60 14.2 13.4 0.242 380 42.0 130 6.5 ab 90 11.2 13.2 0.237 404 42.7 113 5.7 b Pruning (no. times/year) 1 18.3 a 12.1 b 0.202 b 396 a 44.6 a 121 6.7 ab 2 15.9 a 13.8 a 0.254 a 474 a 49.1 a 123 5.0 b 3 8.5 b 13.8 a 0.257 a 263 b 30.7 b 165 8.1 a 'Contender' Nitrogen rate (lbs/acre) 30 14.4 12.1 0.260 369 53.8 155 7.2 60 13.6 11.8 0.234 374 48.5 147 7.3 90 14.0 11.6 0.228 401 49.5 145 7.3 Pruning (no. times/year) 1 22.4 a 10.8 b 0.187 b 427 50.9 139 b 11.9 b 2 15.8 b 12.1 a 0.267 a 367 53.4 153 ab 12.5 a 3 10.0 c 12.6 a 0.269 a 350 47.6 156 a 12.4 a 'Encore' Nitrogen rate (Ibs/acre) 30 8.4 12.1 0.171 435 62.6 139 7.5 60 10.1 12.0 0.177 369 52.0 145 8.8 90 9.7 11.7 0.169 366 53.6 143 9.1 Pruning (no. times/year) 1 17.8 a 10.4 b 0.124 b 482 a 62.3 129 14.3 b 2 8.3 b 12.3 a 0.189 a 357 b 53.4 145 14.0 b 3 7.2 b 13.1 a 0.203 a 330 b 52.4 154 14.6 a ' Numbers within columns followed by the same letter are not significantly different (P=0.05, DMRT). Numbers in columns without letters were not significantly different. 15 ALABAMA AGRICULTURAL EXPERIMENT STATION EFFICACY OF THE SOIL FUMIGANTS TELONE C-35 AND TELONE II ON GROWTH AND SURVIVAL OF PEACH TREES ON A REPLANT SITE Bryan Wilkins, Robert Ebel, Jim A. Pitts, Robert Boozer, and Edward Sikora Because of limited land availability, growers are often forced to plant peach trees on land that had been planted in peach trees for many years. Pathogens build up in soil planted in peach trees, causing replanted trees to perform poorly. Preplant fumigants have been developed to kill soil pathogens. The most popular preplant soil fumigant is methyl bromide because of its high ef- fectiveness; however, methyl bromide is scheduled to be re- moved from the market in the next few years. Telone II (dichloropropene) has been available for many years, but early reports showed methyl bromide and chloroprycrin as being more effective in promoting growth and survival of trees on replant sites. Telone C-35 contains dichloropropene and chlorpycrin, which should be more effective than Telone II in reducing soil pathogens, and may be a suitable replacement for methyl bro- mide. The objective of this study was to compare growth, survival, yield, and disease de- velopment of peach trees planted in soil treated with Telone II and Telone C-35. The experiment was con- ducted on a site that had been planted in peach trees since 1985. In the summer of 1999, the trees were removed. Preplant soil fumigation treatments in- cluded Telone II, Telone'C-35, and an untreated control that was applied in an 8-foot band or broadcasted across the en- tire root zone. Telone II was applied at a rate of 30 gallons per acre and Telone C-35 at a rate of 42 gallons per acre. The experiment was set up as a ran- domized complete block design with five blocks containing four adjacent trees with data collected from the center two trees. The trees in the block were 'Sureprince' on Guardian rootstock planted on a 12- by 20-foot spacing and trained to a four-scaffold Y. The soil was sampled for ring nematode imme- diately before fumigation and before planting. There were no significant differences in the nematode popu- lations throughout the block with the exceptions of spiral and stubby root nematodes; however, there was no consistent trend with respect to treatment (Table 1). Populations of all nematodes were low throughout the experiment. There were no statistical differences in truck cross sectional area or tree vigor for any of the treatments in 2001 (Table 2). There was no statistical differ- ences in number of fruit harvested, yield, and fruit quality. These data demonstrate that these soil fumigants do not have a posi- tive effect on growth and yield of peach when nematode popula- tions are low. TABLE 1. EFFECTS OF Two TELONE FORMULATIONS AT DIFFERENT APPLICATION RATES AND METHODS ON THE POPULATION DENSITIES OF NEMATODES IN 'SUREPRINCE' PEACH 1 Telone C35 Telone C35 Telone II Telone II Nematode Control banded broadcast banded broadcast (42 gal/ac) (42 gal/ac) (30 gal/ac) (30 gal/ac) Spiral 2.4 ab 2 2.5 ab 0.9 b 3.8 a 1.1 b Lesion 2.8 1.3 0.7 1.4 0.8 Ring 5.5 4.9 5.6 2.3 6.2 Stunt 3.1 0.4 2.2 0.4 2.2 Rootknot 0.9 1.8 1.1 1.0 2.2 Dagger 1.0 0.3 0.1 0.5 0.3 Tylenchus 5.8 5.2 2.2 1.9 5.0 Cyst 0 0.1 0 0 0 Reniform 0 0 1.0 1.0 0.1 Stubby Root 0 b 0 b 0 b 0 b 0.2 a 1 Means derived from data taken in 1999, 2000, 2001, and 2002. 2 Mean separation within columns by Duncan's Multiple Range Test p = 0.05, columns without letters were not significantly different. TABLE 2. EFFECTS OF TWO TELONE FORMULATIONS AT DIFFERENT APPLICATION RATES AND METHODS ON THE GROWTH OF 'SUREPRINCE' PEACH Trunk cross- Total fruit Telone Rate Application sectional area harvested Yield (gal/ac) (cm 2 /yr) (no) (lbs) C35 42 Banded 18.12 174 38 C35 42 Broadcast 16.5 . 162 351 II 30 Banded 16.4 196 41 II 30 Broadcast 17.8 138 31 Control 14.1 144 33 Means derived from data taken in 2000, 2001, and 2002. 2Mean separation within columns by Duncan's Multiple Range Test p = 0.05, columns without letters were not significantly different. 16 2001ALUATION OF FNGICIDE SPRAYS FOR PECAN SCAB CONTROL Edward J. Sikora and Jason Burkett Pecan scab, a fungal disease of pecan, is the most limiting disease to pecan production in the Southeast. To control scab, growers must maintain a calendar-based spray program from bud- break through mid August. In 2001, several new fungicides (Stratego) and some experimental compounds (Folicur and Emi- nent) were evaluated for scab control. Results showed that all the fungicide treatments were significantly better than the unsprayed control in controlling leaf and nut scab. The experiment was conducted on a block of 'Desirable' pecan trees at the E. V. Smith Research Center in Shorter, Ala- bama. All of the fungicide treatments (spray programs) were ini- tiated within 10 days after bud-break and followed a 14-day sched- ule throughout the season. Leaf scab was assessed on June 3 and nut scab was rated on September 10. The weather conditions were favorable for leaf scab devel- opment. All the fungicide programs were significantly better than EVALUATION OF FUNGICIDE SPRAY PROGRAMS FOR PECAN SCAB CONTROL, 2001 Application Leaf scab Nut scab Treatment Rate/acre timing I (%) (%) Unsprayed control - - 10.8 a 2 87.5 a Super Tin 80 WP 7.5 oz Sprays 1-8 7.1 b 43.0 bcd Folicur 3.6 F + 6 oz Sprays 1-3 Induce 0.06% then Super Tin 80 WP 7.5 oz Sprays 4-8 6.6 b 49.1 bc Stratego 2 EC 8 oz Sprays 1-3 then SuperTin 80 WP 7.5 oz Sprays 4-8 2.1 c 37.2 de Dodine 65 WP 32 oz Sprays 1-3 then Stratego 2 EC 8 oz Sprays 4-7 then SuperTin 80 WP 7.5 oz Spray 8 3.3 c 42.2 cd Dodine 65 WP 32 oz Sprays 1-3 then Stratego 2 EC 10 oz Sprays 4-7 then SuperTin 80 WP 7.5 oz Spray 8 3.3 c 38.1 de Eminent 125 SL 8 oz + Dodine 65 WP 16 oz Sprays 1-8 3.5 c 50.3 b Eminent 125 SL 1 pint Sprays 1-3 then Agri Tin 80 WP 7.5 oz Sprays 4-8 2.8 c 49.8 bc Super Tin 80WP 3.75 oz + Dodine 65 WP 16 oz Sprays 1-8 7.2 b 33.1 e Enable 75 W 2 oz + Dodine 65 WP 16 oz Sprays 1-8 1.5 c 50.0 b Orbit 4 oz + Dodine 65 WP 16 oz Sprays 1-8 2.3 c 30.6 e LSD (P=0.05) 2.9 7.8 Total of eight sprays. 2 Numbers followed by the same letter are not significantly different. the unsprayed control in con- trolling leaf scab (see table). The Super Tin full-season program, the Folicur/Super Tin block-program and the Super Tin plus Dodine tank- mix program had significantly higher levels of leaf scab than the other seven fungicide programs. All the fungicide pro- grams controlled nut scab significantly better than the unsprayed control. The Su- per Tin plus Dodine tank mix and Orbit plus Dodone tank- mix programs were signifi- cantly better than the other treatments with the exception of the Stratego/Super Tin block program and the Dodine/Stratego (10 ounce)/ Super Tin program. 2001-2002 FRUIT AND VEGETABLERESEARCHREPORT 17 y v i w 18 ALABAMA AGRICULTURAL EXPERIMENT STATION ALTERNATE BEARING IN SATSUMAS Monte Nesbitt, Robert Ebel, William Dozier, Ron McDaniel. and Malcomb Pegues Satsuma mandarin trees are commonly known to experi- ence alternate bearing. Alternating years of heavy and light pro- duction is a serious obstacle to developing a thriving satsuma industry in Alabama because marketing problems arise when the crop fluctuates from year to year. In the 2000 crop season, 11- year-old 'Owari' trees at the Gulf Coast Research and Extension Center (GCREC) in Fairhope, Alabama, averaged 473 pounds of fruit, with some individual trees yielding as much as 792 pounds. In the following year, average tree yield in the same grove was only 157 pounds per tree. Harvest date is a factor that can contribute to alternate bearing in some crops. A satsuma tree with 792 pounds of fruit may actually have some 3,900 individual fruit on it (five fruit per pound average), which can take a great deal of time to hand harvest, especially in a commercial planting with 100 or more trees per acre. Ifremoval of the fruit is extended over a 30- to 60- day period, the fruit that remain on the tree continue to act as sinks for carbohydrates, depressing the trees' ability to build up carbohydrates essential to produce a normal crop the following year. In 1999 and 2000, a study was conducted at the GCREC to determine the effects of late fruit harvest on alternate bearing. Six trees were completely harvested on each of three dates: Novem- ber 1, December 1, or January 1, and yield was measured the following season. Yield was significantly reduced in 2000 only when harvest was delayed until January 1. The lower yield for EFFECT OF ARVESTDATE ON YIELD OF SATSUMA, 2000 AND 2001 Harvest date Pounds/tree 2000 Pounds/tree 2001 November 1 576 271 December 1 559 122 January 1 419 232 January-harvested trees, although significant, is not dramatic, and most growers harvest most of their fruit before January 1. Thus normal harvesting, which usually begins in early Novem- ber and extends into mid-December, may not be a major contrib- uting factor to alternate bearing, at least on a moderately large crop. The average yield of trees in this study in 1999 was 372 pounds, which may not have been enough to cause severe alter- nate bearing. The 2000 crop was larger and alternate bearing did occur in 2001 (see table). Although it appeared that yields in 2001 were reduced by the December harvest date, the differences were not statistically significant, due to a high degree of variation in yield in the test trees. The variation was caused by freeze damage to the foliage in the winter of 2000-01. Trees in the study that lost less than 40% of their foliage produced 300 pounds per tree in 2001, and those that lost more produced only 100 pounds per tree. Winter injury to foliage combined with heavy production and delayed harvest are major causes for alternate bearing in satsumas. SATSUMA DISEASE SuiRVEY OF ALABAMA Kathy McLean, Edward Sikora, Robert Ebel, S. L. Burchett, and Monte Nesbitt Satsuma mandarins are a type of citrus that are character- istically loose-skinned, brightly colored, and have a very flavor- ful taste. Citrus trees were introduced into Florida by Spanish explorers in 1565. Small satsuma orchards developed along the Gulf of Mexico by the late 1800s. However, severe winters late in the century severely diminished the numbers of trees surviving. Satsuma acreage did increase again with milder weather and by the early 1920s, approximately 18,000 acres of satsumas were growing in the coastal regions of Alabama, Florida, and Louisi- ana. In Alabama, satsumas were considered to produce more consistent crops, ripen within the growing season, and require less cold protection than other citrus. However, satsuma trees are not tolerant to extended freezing temperatures and multiple severe winters in the 1930s and 1940s depreciated the satsuma acreage. Alabama currently has a new and emerging commercial satsuma industry in the Mobile Bay area. But the type.and extent of disease incidence in the growing region is currently not well characterized. A survey was conducted in November of 2000 and 2001 to determine the disease incidence and pathogens present in the satsuma acreage in Alabama. Seven orchards representing the majority of the commer- cial acreage were surveyed for symptomatic fruit and foliage in 2000 and 2001. Samples of fruit and foliage withdisease symp- toms were collected from each orchard, labeled, and immediately placed in plastic bags in a cooler of ice. Samples were trans- ported back to the laboratoryand placed in a cold room at 40C. Within 24 hours samples were photographed and disease symp- toms described. Tissue sections were excised from the leaves and fruits of each diseased sample collected. Tissue sections were surface-sterilized and aseptically plated on acidified potato dextrose agar. Plates were incubated at 24 0 C for 10 days, during and after which colonies of fungi growing from the tissue were identified, or subcultures were prepared for later identification. ALABAMAAGRICULTURAL ExPERIMENT STATION18 2001-2002 FRUIT AND VEGETABLE RESEARCH REPORT 19 Seven diseases were identified. Alternaria leaf spot and brown spot of satsuma caused by Alternaria alternata Fr. (Kiessler) pv. citri was found in 85 and 75% of the orchards surveyed in 2000 and 2001, respectively (see table). Mycelia ofA. alternata pv. citri is dark olive in color and grows rapidly at 240C. Conidia are dark, obclavate to elliptical with both cross and longitudinal septa borne in apical chains. On leaves the lesions appeared as circular or irregular blighted areas that were often surrounded by a yellow halo. On the mature fruit the symptoms varied from small dark specks to large black lesions sometimes surrounded by a yellow halo. Fruit blemishes will significantly reduce the market price and can be an economical factor in pro- duction. Anthracnose, caused by Colletotrichum gloeosporiodes (Penz.) Penz. & Sacc., was found in 85 and 50% of the orchards surveyed in 2000 and 2001, respectively. Mycelia of C. gloeosporiodes is gray to brown in color with spore masses that appear salmon in color. Acervuli are produced which are disk- shaped with dark setae among the conidiophores. Conidia are abundant, hyaline, one-celled, oblong to fusiform. Symptoms on leaves include necrotic areas which may coalesce. Anthracnose on mature fruit are usually considered secondary but appear as brown to black spots or tear-stain blemishes. Blue and green molds of fruit caused by Penicillium spp. were found in 71 and 87% of the orchards in 2000 and 2001, respectively. Mycelia of Penicillium spp. appeared blue to blue gray or olive green in the center surrounded by white to light- colored areas. Conidia are one-celled, globose in shape and borne on phialids in dry basipetal chains. Symptoms on mature fruit include soft discolored areas 5 to 10 centimeters in diameter, to the entire fruit surface being encompassed by mycelium and spores. PERCENT OF SATSUMA ORCHARDS AND SAMPLES INFESTED IN THE LABAMA SURVEY, 2000 AND 2001 Disease Orchards infested Samples infested 2000 2001 2001 Alternaria leaf spot 85 75 43 Anthracnose 85 50 22 Penicillium fruit rot 71 87 27 Melanose 85 25 8 Twig die back 71 37 4 Distorted fruit 29 75 15 Mean 73 58 20 Melanose of mature fruits, caused by Phomopsis citri H. Fawc. (Sacc.) Traverso & Spessa., was found in 85 and 25% of the orchards in 2000 and 2001, respectively. Phomopsis citri pro- duces white to cream, ropy mycelia with dark pycnidia in scat- tered stroma. Conidia are ovoid to fusoid (alpha) or filiform and curved (beta). Symptoms on mature fruit appear as small, brown discrete specks or streaks of tear stains across the fruit surface. Twig tip dieback and distorted fruit were observed in 71 and 37% and 29 and 75% of the orchards in 2000 and 2001, re- spectively; however, no causal agent for these disorders was determined. Overall, 73 and 58% of the orchards were infested with at least one fungal pathogen in 2000 and 2001, respectively. How- ever, the incidence and severity of fruit and foliar diseases of satsuma in each orchard were relatively light. The 2000 and 2001 seasons were considered dry years for Alabama, which may have resulted in low disease pressure. EVALUATION OF FUNGICIDES FOR BOTRYTIS GRAY MOLD CONTROL ON STRAWBERRIES, 2002 Edward Sikora and Jim A. Pitts Botrytis gray mold is a fungal disease that causes a fruit rot of strawberries in Alabama. The loss of the fungicides Benlate and Ronilan in recent years has reduedu the number of fungi- cides available to growers to combat this disease. In 2001-2002, a number of fungicides in various spray programs were evaluated to determine their efficacy against this disease. Results indicate that all the fungicide programs evaluated controlled gray mold equally well. There also was no apparent difference among the fungicides in controlling anthracnose, a fungal disease that was a common problem in strawberry production fields in 2002. The test was conducted at the Chilton Research and Ex- tension Center in Clanton, Alabama. The strawberry variety 'Chan- dler' was transplanted to the field in October of2001. Plants were grown on raised beds with black plastic mulch and drip irrigation. Treatments were initiated in the spring of 2002 at 10% bloom. There were a total of eight fungicide applications made on the following days: March 20, March 28, April 3, April 10, April 17, April 26, May 1, and May 8. Total marketable fruit weight and percent fruit with symptoms of Botrytis gray mold and/or anthra- cnose were determined. All the fungicide programs had higher marketable fruit weights than the unsprayed control (see table). The program that alternated Rovral with Topsin M plus Captan produced ap- proximately 8% less marketable fruit than the Topsin M plus Captan full season program. There were no apparent differences among the fungicide programs in their ability to control Botrytis gray mold or anthracnose. 2001-2002 FRUIT AND VEGETABLERESEARCHREPORT 19 20 ALABAMA AGRICULTURAL EXPERIMENT STATION EVALUATION OF FUNGICIDES FOR CONTROL OF BOTRYTIS GRAY MOLD AND ANTHRACNOSE OF STRAWBERRIE, 2002 Fungicide treatment/ Marketable Botrytis gray Fruit Rate/acre fruit (g) mold (%) anthracnose (%) Unsprayed control 4317 11.0 2.3 Topsin M 1 Ib + Captan 50WP 4 Ib 6360 > 1.0 5.4 Topsin M 1 Ib + Captan 50WP 4 lb ALTERNATED WITH Elevate 50WG 1.5 Ib +Captan 50WP 4 Ib 6164 >1.0 5.5 Topsin M 1 lb + Captan 50WP 4 Ib ALTERNATED WITH Switch 62.5 WG 14 oz 6336 >1.0 4.1 Topsin M 1 lb + Captan 50WP 4 Ib ALTERNATED WITH Rovral 50WP 2 lb 5325 2.9 4.1 Topsin M 1 lb + Captan 50WP 4 Ib ALTERNATED WITH Cabrio EG 14 oz 5854 >1.0 5.5 ALABAMA AGRICULTURAL ExPERIMENT STATION20 2001-2002 FRUIT AND VEGETABLE RESEARCH REPORT VE GE TABLE PAP"IERS EVALUATION OF FUNGICDES FOR CONTROL OF ALERNARIA LEAF SPOT OF CABBAGE Edward Sikora and Arnold Caylor More than 800 acres of cabbage are planted annually in Alabama with an estimated value of $2.5 million. The main dis- ease problem of cabbage is Alternaria leaf spot. Production of marketable cabbage can be severely affected if this disease goes uncontrolled. Alternaria causes a leaf spot that can result in sig- nificant defoliation in the field. In storage, the disease can pre- dispose infected cabbage heads to soft rot bacteria. Fungicides with the active ingredient chlorothalonil or maneb are labeled for control ofAlternaria leaf spot on cabbage but are only moderately effective against the disease. For this reason, alternative products were evaluated in this study through the IR-4 program. The trial was conducted in 2001 at the North Alabama Hor- ticulture Research Center in Cullman, Alabama. Cabbage was transplanted in mid September. Each treatment consisted of a one-row plot 15 feet long. The experiment consisted of four treat- ments, replicated five times, in a randomized complete block de- sign. Disease ratings were taken in late November and early De- cember. Cabbage was harvested and total number of marketable heads, total marketable weight, and average marketable head weight were determined. Alternaria leaf spot was observed in trace amounts on one replication of the untreated control. There were no significant EVALUATION OF UNGICIDES FOR CONTROL OF FOLIAR DISEASE OF CABBAGE, 2001 Treatments Marketable Marketable Average rate/acre 1 heads weight head weight (no/plot) (Ib/plot) (Ib) Untreated 9.75 a 2 63.57 a 6.55 a Bravo Weather Stik 10.50 a 67.15 a 6.40 a 1.50 pt BAS 500 9.75 a 59.92 a 6.15 a 0.75 lb BAS 500 10.50 a 67.02 a 6.35 a 1.00 lb 1 Bravo Weather Stik and the BAS 500 treatments were applied three times at seven-day intervals beginning when Alternaria leaf spot first appeared in late November. 2 Means with the same letter are not significantly different. differences among the treatments in terms of total number of marketable heads produced, total marketable head weight, or av- erage marketable head weight (see table). No symptoms of phy- totoxicity among the fungicide treatments were observed. E FFECT OF NITROGEN SOURCE ON QUALITY AND YIELD OF 'LAROUGE' IRISH POTATO Joseph Kemble, Edgar Vinson, and Tony Dawkins Growers have often asked the question "How does nitro- gen source affect various vegetable crops?" In the case of toma- toes, research has shown that tomatoes perform better (improved quality and fewer fruit defects) when they are fertilized solely with nitrate-nitrogen (potassium nitrate, calcium nitrate) as op- posed to ammonium-nitrogen (ammonium nitrate). This experi- ment was initiated to determine if nitrogen source would affect the quality and yield of Irish potato. After one season, no differ- ences were found among the treatments; however, this experi- ment will be repeated. An experiment was initiated at the Sand Mountain Research and Extension Center in Crossville, Alabama, to determine the effect of various percentages of preplant and sidedress applica- tions of ammonium nitrate and/or calcium nitrate on Irish potato. There werewere four treatments: (1) 100% calcium nitrate (preplant)/ 100% calcium nitrate (sidedress); (2) 100% calcium nitrate (pre- plant)/100% ammonium nitrate (sidedress); (3) 100% ammonium nitrate (preplant)/100% ammonium nitrate (sidedress); and (4) 100% ammonium nitrate (preplant)/100% calcium nitrate (sidedress). Soil pH and fertility (except for nitrogen) were adjusted based on soil test results from the AU Soil Testing Lab. Preplant nitrogen treatments were applied to meet 50% of the crop's nitro- gen need. Seed pieces of 'LaRouge' were cut, treated with fungi- cide, and set on March 15, 2002. The remaining nitrogen was applied in two sidedress treatments, the first sidedress one month 21 after planting, and the second about one month after the first able yield, tuber sizes, or culls among the four treatments (see sidedress. table). In fact, no differences were found among the treatments Vines were rolled prior to applying Diquat. Tubers were for skin set or skin color (data not shown). Some lack of differ- dug on July 3, 2002 and then graded and weighed. Tubers were ence could be do to the extended hot, dry weather during most of separated based on size as size A potatoes (diameter greater than the late spring through midsummer before the tubers were har- or equal to 1 7/8 inches), size B potatoes (diameter 1 1/2 to 1 1/7 vested. This experiment will be repeated next season. inches), or culls (diameter of less than 1 1/7 inches). Total _ marketable yield equaled the EFFECT OF NITROGEN SOURCE ON QUALITY AND YIELD OF 'LAROUGE IRISH sum of the size A and B pota- POTATO AT SAND MOUNTAIN RESEARCH AND EXTENSION CENTER, toes for a particular treatment. CROSSVILLE, AL, 2002 In addition to grading and weighing, skin set and skin Total color were evaluated, marketable No differences were Treatment yield Size A 1 Size B Cull 3 found in terms of total market- (lb/ac) (lb/ac) (lb/ac) (lb/ac) (3) Am nit/Am nit 17,739 a 4 15,397 a 2,341 a 478 a (4) Am nit/Ca nit 17,249 a 14,944 a 2,305 a 472 a (1) Ca nit/Ca nit 17,067 a 15,046 a 2,021 a 823 a (2) Ca nit/Am nit 15,936 a 13,818 a 2,118 a 424 a Size A potatoes have a >1 7/8 inch diameter. 2 Size B potatoes have a 1 1/2 - 1 1/7 inch diameter. SCull potatoes are those with a diameter of less than a 1 1/7 inch. 4 Values followed by the same letter are not significantly different according to Range Test (p<0.05). Duncan's Multiple EFFECT OF SPLIT APPLICATION OF DESICCANT AND VINE ROLLING ON QUALITY AND YIELD OF 'LAROUGE' IRISH POTATO Joseph Kemble, Edgar Vinson, and Tony Dawkins Growers in Alabama commonly use a vine desiccant, such as Diquat, on Irish potatoes in order to kill the actively growing vines to facilitate harvesting. These desiccants also aid in pro- moting skin set - a toughening of the potato skin, which helps extend the post-harvest life of the tuber. In other potato-produc- ing regions of the United States, growers often use split applica- tions of desiccants (with reduced rates) and have found favor- able results and fewer problems with stem-end browning of tu- bers. In addition, vine rolling, in combination with split applica- tions of desiccant, has also been found to aid in skin set. This research was initiated to determine if these procedures could aid Irish potato growers inAlabama. In 2002, research indicated that marketable yields of potatoes sprayed with spit applications of desiccant were greater, although not statistically, than those treated with a single higher rate of desiccant. This work will be continued in 2003. This experiment was initiated at the Sand Mountain Re- search and Extension Center in Crossville, Alabama, to deter- mine the effect of split applications of the desiccant Diquat and vine rolling on the quality and yield of'La Rouge' Irish potatoes. There were four treatments: (1) single application of Diquat (1 quart per acre); (2) split application of Diquat (1 pint per acre, then a second application four days after the first); (3) single application of Diquat (1 quart per acre), vine rolling; and (4) split application of Diquat (1 pint per acre, then a second application four days after the first), vine rolling. Soil pH and fertility (except for nitrogen) were adjusted based on soil test results from the AU Soil Testing Lab. Preplant nitrogen treatments were applied to meet 50% of the crop's nitro- gen needl. Seed pieces of'LaRouge' were cut, treated with fungi- cide, and set on March 15, 2002. The remaining nitrogen was applied in two sidedress treatments, the first sidedress one month after planting, and the second about one month after the first sidedress. Vines in treatments 3 and 4 were rolled on June21. Diquat was applied on June 25 on all treatments at either 1 quart per acre or 1 pint per acre. A second application of Diquat was made on June 29 to treatments 2 and 4 (the 1-pint-per-acre treatments). Tubers were dug on July 3 and then graded and weighed. Tu- bers were separated based on size as size A potatoes (diam- 22 ALABAMA AGRICULTURAL ExPERIMENT STATION eter greater than or equal to 1 7/8 inches), size B potatoes compared to treatments 1 and 3 (single application of Diquat) (diameter of 1 1/2 to 1 1/7 inches), or culls (diameter of less (see table). Treatments 1 and 3 (single application of Diquat) than 1 1/7 inches). Total marketable yield equaled the sum of produced the lowest marketable yield, although it was not statis- the size A and B potatoes for a particular treatment. tically lower (see table). Based on these results, this experiment Treatments 2 and 4 (spilt applications of Diquat) showed a will be repeated in 2003. slight, although not significant, increase in total marketable yields EFFECT OF SPLIT APPLICATION OF DESICCANT AND VINE ROLLING ON QUALITY AND YIELD OF 'LAROUGE' IRISH POTATO AT SAND OUNTAIN RESEARCH AND EXTENSION CENTER, CROSSVILLE, AL, 2002 Total marketable Treatment yield Size A' Size B 2 Cull 3 (lb/ac) (lb/ac) (Ib/ac) (Ib/ac) Treatment 2 19,306 a 4 16,964 a 2,341 ab 581 a Treatment 4 19,027 a 16,256 a 2,771 a 532 a Treatment 3 18,894 a 16,571 a 2,323 ab 696 a Treatment 1 16,202 a 14,617 a 1,585 b 484 a 1 Size A potatoes have a >1 7/8 inch diameter. 2 Size B potatoes have a 1 1/2 - 1 1/7 inch diameter. 3 Cull potatoes are those with a diameter of less than a 1 1/7 inch. 4 Values followed by the same letter are not significantly different according to Duncan's Multiple Range Test (p<0.05). EVALUATION OF SYNTHETIC AND BIOLOGICAL FUNGICIDES FOR CONTROL OF POWDERY MILDEW ON PUMPKIN Edward Sikora and Tony Dawkins Powdery mildew is a common fungal disease ofpumpkin in Alabama. Powdery mildew reduces yield by decreasing the size and number of fruit or the length of time the crop has to mature. In 2002, both commercially available synthetic fungicides (Quadris, Bravo, Ridomil, and Microthiol Disperss) as well as two experimental biological fimungicides (QRD 283 and 286) were evaluated. Results showed that Microthiol Disperss (a sulfur- based product) controlled powdery mildew better than the other products evaluated. This trial was conducted at the Sand Mountain Research Station in Crossville, Alabama. The variety 'Appalachian' was di- rect seeded into the field in late June. The experiment consisted of six treatments, replicated four times, in a randomized complete block design. Each treatment/replication consisted of a one-row plot, 20 to 25 feet long. The fungicide programs were started when the vines began to run. Fungicides were applied every seven days (some treatments alternated between two products). Disease rat- ings were taken approximately 10 days before harvest. Yield data were yet not available for inclusion in this publication. Powdery mildew was the dominant disease observed in the trial (low levels of downy mildew were also noted). The EVALUATION OF FUNGICIDES FOR CONTROL OF POWDERY MILDEW, SAND MOUNTAIN, ALABAMA, 2002 Treatments/rate' % Powdery mildew Unsprayed control Quadris (11 oz/acre) ALTERNATED WEEKLY WITH Bravo Ultrex (2.7 lb./acre) Microthiol Disperss 80 DF (8 lb./acre) QRD 286 AS (1% by volume) QRD 283 WP (4 Ib./acre) QRD 283 WP (4 lb./acre) ALTERNATED WEEKLY WITH Ridomil Gold Bravo WP (3 Ib./acre) 75.5 24.7 17.2 49.2 32.2 27.0 'Spray programs were initiated at vine run and sprayed every 7 days. Microthiol Disperss treatment had the lowest incidence of pow- dery mildew among the treatments (see table). Microthiol Disperss is a sulfur-based product, and sulfur fungicides have proven to be very effective in controlling powdery mildew in previous trials 2001-2002 FRUIT AND VEGETABLERESEARCHREPORT 23 24 ALABAMA AGRICULTURAL EXPERIMENT STATION in Alabama. The Quadris/Bravo treatment and both QRD 283 treatments also performed well in this trial. Microthiol Disperss and other sulfur products do a good job in controlling powdery mildew on pumpkins. Growers should be aware, however, that sulfur is only effective against powdery mildew, not downy mildew. Growers need to scout pumpkin fields weekly to determine if wet weather diseases such as downy mil- dew are developing. If diseases other than powdery mildew ap- pear, the grower must begin spraying broad-spectrum materials such as Bravo and Quadris to reduce damage from these other diseases. EVALUATION OF IPM SPRAY PROGRAMS FOR CONTROL OF FOLIAR DISEASES OF PUMPKIN, 2001 Edward Sikora, Josepb Kemble, and Tony Dawkins Downy mildew and powdery mildew are two common fun- gal diseases of pumpkin in Alabama. Powdery mildew is most common in dry years while downy mildew is considered a wet- weather disease. Both diseases reduce yield by decreasing the size and number of fruit or the length of time the crop has to mature. In 2001, a powdery-mildew-tolerant pumpkin variety was evaluated with two Integrated Pest Management (IPM) strate- gies. The two-IPM strategies were as follows. First, the fungicide spray program was initiated if and when disease was first ob- served in the field by way of a twice-a-week-scouting program; and second, the period between fungicide applications was ex- tended from seven days to 10 to 12 days. Results were greatly affected by the appearance of a new disease to Alabama. The fungal disease Plectosporium blight developed early in our trials and was confused with spray burnm by the researchers. This was the first report of Plectosporium blight in Alabama. This resulted in greater defoliation and lower yields in the two scouting treatments than in the standard seven- day fungicide program. This trial was conducted at the Sand Mountain Research and Extension Center in Crossville, Alabama. The variety 'Mer- lin' was direct seeded into the field on June 20. The experiment consisted of five treatments, replicated five times in a random- ized complete block design. Each treatment/replication consisted of a one-row plot, 30 feet long. The experiment compared four fungicide programs and an unsprayed control. The four fungi- cide programs consisted of Quadris alternated with Bravo Ultrex on a seven-day or a 10- to 12-day schedule with programs initi- ated at vine run or when disease was first observed in the field. Disease ratings were taken on September 12. EFFECTIVENESS OF IPM PROGRAMS ON DISEASE DEVELOPMENT ON A POWDERY-MILDEW TOLERANT PUMPKIN VARIETY, SAND MOUNTAIN, 2001 Fungicide timing/ Downy Plectosporium When initiated ' mildew blight Unsprayed control 48.0 43.0 7 days/vine run 12.0 2.0 10-12 days/vine run 29.0 9.0 7 days/scouting 28.0 37.0 10-12 days/ scouting 32.0 34.0 1 Treatments were sprayed on a seven-day or 10-12 day inter- val. Treatments were initiated at vine run or at first appearance of disease when following a biweekly scouting program. The fungicide program for all treatments, with the exception of the unsprayed control, was Quadris alternated with Bravo Ultrex. Results showed that 'Merlin' grown following the seven- day fungicide schedule initiated at vine-run had the lowest lev- els of downy mildew and Plectosporium blight (see table) and the highest marketable yields (data not shown). No differences in yield were observed among the other three fungicide spray pro- grams. Three to four fewer fungicide applications were required when following the scouting program resulting in lower input costs except for the scouting, possibly offsetting loss in market- able yields. This trial demonstrates that growers/field scouts must be aware of all potential plant diseases and pests that may dam- age a crop. ALABAMAAGRICULTURAL ExPERIMENT STATION24 2OMPARISON OF TERRARY G EPANDTERRAPY TO METHYL BROMIDE As GROWTH PROMOTERS FOR TOMATO, 2001 Edward Sikora and Arnold Caylor TerraPy B and TerraPy G are organic products that have been shown to reduce plant stress and plant disease activity by stimulating soil microbiological activity and promoting nutrient uptake of various horticultural and agronomic crops in Europe and Asia. The objective of this study was to determine if the products would promote growth and boost yields of tomato in Alabama. Results show that four weeks after application, plants treated with TerraPy G had a higher health rating than those treated with TerraPy B. Unfortunately, no significant differences were observed in terms of total marketable fruit weights or in fruit size among the treatments. This experiment was conducted at the North Alabama Hor- ticultural Research Center in Cullman, Alabama. Tomato trans- plants were set in the field on June 6. Plants were grown on raised beds covered with white plastic mulch and drip irrigated. The experiment consisted of four treatments replicated six times in a randomized complete block design. Each treatment replication consisted of a one-row plot, 20 feet long. The methyl bromide treatment was applied at the standard commercial rate approxi- mately one month before transplanting. TerraPy B (20 grams per square meter) and TerraPy G (20 grams per square meter) were applied as a soil drench at transplanting. Plots were sprayed weekly with a tank mix of mancozeb plus copper for foliar disease control. Plots were evaluated for plant health (height, color, and vigor) four and eight weeks after transplanting. Tomatoes were PLANT HEALTH RATINGS AND TOTAL MARKETABLE EIGHT OF TOMATOES, 2001 Treatment Plant health Total marketable ratings weight 4 weeks (Ib) TerraPy G (20 g/m 2 ) 3.4 a 1 108.3 a TerraPy B (20 g/m 2 ) 2.6 b 90.5 a Methyl bromide 3.2 ab 122.3 a Control (water only) 3.0 ab 101.1 a SNumbers followed by the same letter are not significantly dif- ferent. harvested weekly at the breaker stage and graded for size then weighed by size (extra large, large, medium, and small) and total marketable weights were determined. TerraPy G had the highest plant health rating among the treatments (see table), significantly higher than the TerraPy B 20 treatment four weeks after transplanting. There were no differ- ences among treatments eight weeks after transplanting. There were few significant differences in yield among treatments (data not shown). The methyl bromide treatment produced a signifi- cantly higher medium fruit weight than TerraPy B. There were no significant differences in total marketable yield among treatments. 2001-2002 FRUIT AND VEGETABLERESEARCHREPORT 25 ALABAMA AGRICULTURAL EXPERIMENT STATION EVALUATION OF TERRA Y G AT MULTIPLE RATES AS A GROWTH PROMOTER FOR TOMATO, NORTH ALABAMA, 2001 Edward J. Sikora and Arnold Caylor TerraPy G is an organic product that has been shown to reduce plant stress and plant disease activity by stimulating soil microbiological activity and promoting nutrient uptake of vari- ous horticultural and agronomic crops in Europe and Asia. The objective of this study was to determine the optimum rate of TerraPy G as a growth promoter on tomato. Results indicate that TerraPy G at the high rate (20 grams per square meter) may be phytotoxic to tomato, based on growth ratings at four and eight weeks after application. The low (2 grams per square meter) and high (20 grams per square meter) rates of TerraPy G produced significantly lower marketable fruit weights than the control and the 5 and 10 grams per square meter TerraPy G treatments. This experiment was conducted at the North Alabama Hor- ticultural Research Center in Cullman, Alabama. Tomato trans- plants were set in the field on May 25. Plants were grown on raised beds covered with white plastic mulch and drip irrigated. The experiment consisted of five treatments replicated five times in a randomized complete block design. Each treatment replica- tion consisted of a one-row plot, 20 feet long. The TerraPy G treatments were applied as a soil drench at transplanting. Plots were sprayed weekly with a tank mix of mancozeb plus copper for foliar disease control. Plots were evaluated for plant health (height, color, and vigor) four and eight weeks after transplanting. Tomatoes were harvested weekly at the breaker PLANT HEALTH RTINGS AND TOTAL MARKETABLE WEIGHT OF TOMATOES, CULLMAN 2001 Treatment Plant health Total marketable -ratings- weight 4 weeks 8 weeks (Ib) TerraPy G (2 g/m 2 ) 3.2 a 1 3.0 a 139.6 b TerraPy G (5 g/m 2 ) 3.2 a 3.0 a 159.9 ab TerraPy G (10 g/m 2 ) 3.2 a 2.8 a 164.9 ab TerraPy G (20 g/m 2 ) 2.0 b 2.2 b 137.0 b Control (water only) 3.6 a 3.0 a 178.9 a ' Numbers followed by the same letter are not significantly dif- ferent. stage and graded for size then weighed by size (extra large, large, medium, and small) and total marketable weights were determined. TerraPy G (20 grams per square meter) had the lowest plant health rating at both four and eight weeks after transplanting (see table). The low (2 grams per square meter) and high (20 grams per square meter) rates of TerraPy G produced signifi- cantly lower total marketable fruit weights than the control and the 5 and 10 grams per square meter TerraPy G treatments. Based on the results of this trial and an identical trial at the Sand Moun- tain Research and Extension Center, TerraPy G at any tested rate did not appear to promote plant health or improve yield produc- tion of tomatoes in Alabama. 26 2001-2002 FRUIT AND VEGETABLE RESEARCH REPORT EVALUATION OF TERRAPY G AT MULTIPLE ]RATES AS A GROWTH PROMOTER FOR TOM AT, SAND MOUNTAIN, 2001 Edward J. Sikora and Tony Dawkins TerraPy G is an organic product that has been shown to reduce plant stress and plant disease activity by stimulating soil microbiological activity and promoting nutrient uptake of vari- ous horticultural and agronomic crops in Europe and Asia. The objective of this study was to determine the optimum rate of TerraPy G as a growth promoter on tomato. Results indicate that TerraPy G at any of the tested rates had little effect on growth and yield of tomato. This experiment was conducted at the Sand Mountain Re- search and Extension Center in Crossville, Alabama. Tomato trans- plants were set in the field on May 24. Plants were grown on raised beds covered with white plastic mulch and drip irrigated. The experiment consisted of five treatments replicated five times in a randomized complete block design. Each treatment replica- tion consisted of a one-row plot, 20 feet long. The TerraPy G treatments were applied as a soil drench at transplanting. Plots were sprayed weekly with a tank mix of mancozeb plus copper for foliar disease control. Plots were evaluated for plant health (height, color, and vigor) five weeks after transplant- ing. Tomatoes were harvested weekly at the breaker stage and graded then weighed by size (extra large, large, medium, and small) and total marketable weights were determined. PLANT HEALTH RATINGS AND TOTAL MARKETABLE EIGHT OF TOMATOES, SAND MOUNTAIN 2001 Treatment Plant health Total marketable ratings weight 5 weeks (Ib) TerraPy G (2 g/m 2 ) 3.2 a 1 167.2 a TerraPy G (5 g/m 2 ) 3.2 a 147.2 a TerraPy G (10 g/m 2 ) 2.8 a 165.8 a TerraPy G (20 g/m 2 ) 2.6 ab 160.2 a Control (water only) 2.0 b 165.7 a SNumbers followed by the same letter are not significantly dif- ferent The control treatment had the lowest plant health rating five weeks after transplanting; however, the ratings were not significantly different from the TerraPy G treatments at 10 and 20 grams per square meter (see table). There were no significant differences among treatments in total marketable fruit weight. Based on the results of this trial and an identical trial at the North Alabama Horticultural Research Center in Cullman, TerraPy G at any tested rate did not appear to promote plant health or improve yield production of tomatoes in Alabama. GREENHOUSE TOMATO TRIAL REVEALS FEW DIFFERENCES Joseph Kemble, Edgar Vinson, Floyd M. Woods, and Raymond Thomas Production of greenhouse tomatoes is becoming a popular business among vegetable growers and nursery owners. The quality of greenhouse tomatoes and their off season availability help insure a steady market. Greenhouse or hydroponic tomato production is labor in- tensive and requires steadfast, daily oversight and care. Many variables must be monitored and choices made to insure a pro- ductive crop. One such choice is that of variety selection. For greenhouse production, tomatoes specifically bred for that pur- pose, not for field production, should be used. A greenhouse tomato variety trial was conducted at Miss Emily's Hydroponic Tomatoes in Coker, Alabama, in the spring of 2002. Six-week-old tomato seedlings were planted on February 18, 2002. Tomato seedlings were planted into 2-cubic-foot poly- ethylene bags filled with coconut coir. Three bags containing two plants represented each variety. Tomato varieties were repli- cated four times and were arranged in a randomized complete block. Tomatoes were harvested once weekly fromApril 4 through May 3 for a total of six harvests. Tomatoes were weighed and graded. Grades and corresponding fruit diameters (D) of fresh market tomatoes were adapted from the Tomato Grader's Guide (Circular ANR 643 from the Alabama Cooperative Extension Sys- tem) and graded as extra-large (D greater than 2.9 inches), large (D greater than 2.5 inches), medium (D greater than 2.3 inches), and small (D less than 23 inches). Marketable yield was calcu- lated by combining the extra-large, large, medium, and small grades (Table 1). Culled tomatoes were separated based on physiologi- cal disorders such as concentric cracking, radial cracking, cat- facing, blossom end rot, and russeting. In marketable yield, marketable number, and size distribu- tion categories, there were no significant differences among va- rieties (Table 1). Total cull weights were significantly different among varieties (Table 2). 'Style' had significantly higher total cull weights than '73-36RZ' and 'Blitz' but was similar in cull weight to all other varieties. 'Grace' and 'Blitz' also differed in 27 28 ALABAMA AGRICULTURAL EXPERIMENT STATION total cull weight with 'Grace' having a higher total cull weight than 'Blitz'. Blossom end rot was highest with 'Style' when com- pared to 'Blitz' but when compared to other varieties there were no differences. 'Mariachi' had a higher incidence of radial crack- ing than 'Blitz,' while the remaining varieties were similar to 'Blitz' in terms of cracking. There were few differences among varieties in physiochemical attributes such as pH and soluble solids (obrix). 'Style' was similar to 'Grace', 'Trust', and 'Blitz' but had a signifi- cantly lower pH than 'Mariachi' and '73-36RZ'. 'Mariachi' and '73-36RZ' were similarto 'Grace', 'Trust', and 'Blitz'. Soluble sol- ids content of '7336RZ' were higher than 'Blitz' but similar to all other varieties. When compared to standard varieties 'Blitz', 'Grace', and 'Trust', the other varieties differed little. Of the three standards, 'Blitz' appeared to be the better performer overall when compari- sons were made with newer Varieties. Though few differences existed among varieties in terms of quality (cull fruit and physiochemical attributes), there were no real differences in mar- ketable yield. TABLE 1. MARKETABLE YIELD OF TOMATO FRUIT FROM A GREENHOUSE TOMATO VARIETY TRIAL, COKER, ALABAMA 20021 Variety Marketable Marketable Extra large Large Medium Small Average yield yield yield yield yield yield weight/fruit (Ib/plot) (no/plot) (lb/plot) (lb/plot) (Ib/plot) (lb/plot) (oz) Blitz 33 a 2 68 a 21 a 7.88 a 3.18 a 0.84 a 7.98 a Mariachi 33 a 64 a 20 a 8.87 a 3.67 a 0.82 a 8.35 a Trust 31 a 51 a 20 a 5.81 a 4.35 a 1.00 a 9.53 a 73-36RZ 28 a 58 a 18 a 7.47 a 2.84 a 0.63 a 7.89 a Grace 25 a 54 a 14 a 7.12 a 3.10 a 0.82 a 7.56 a Style 22 a 57 a 11 a 7.49 a 3.02 a 0.71 a 6.41 a Significance 3 ns ns ns ns ns ns ns 1Trial was conducted at Miss Emily's Hydroponic Tomatoes. Yields are based on six-plant plots. 2 Numbers followed by different letters are significantly different based on Duncan's Multiple Range Test. 3 ns indicates not significant at p=0.05. TABLE 2. CULL DISTRIBUTION AND PHYSIOCHEMICAL ATTRIBUTES OF TOMATO FRUIT FROM A GREENHOUSE TOMATO VARIETY TRIAL, COKER, ALABAMA 20021 Variety Catfacing Concentric Blossom Radial pH Soluble Total cull cracking end rot cracking Russeting solids (Ib/plot) (Ib/plot) (lb/plot) (Ib/plot) (Ib/plot) (Ib/plot) (?brix) Style 11.4 a 2 - 0.5 1.0 a 4.4 ab 5.0 4.72 a 3.10 b Grace 9.2 ab - 0.5 0.2 ab 3.1 ab 4.5 4.75 ab 3.35 ab Mariachi 8.4 abc - - 0.1 ab 4.5 a 2.6 4.75 ab 3.30 ab Trust 8.2 abc 0.2 0.2 0.1 ab 4.2 ab 2.8 4.77 ab 3.20 b 73-36RZ 6.4 bc 0.1 1.4 - 2.7 ab 1.5 4.80 b 3.35 ab Blitz 5.0 c 0.3 0.1 0.5 ab 1.2 b 2.3 4.82 b 3.70 a Significance3 * ns ns ** ** ns ** ns Trial was conducted at Miss Emily's Hydroponic Tomatoes. Yields are based on six-plant plots. 2 Numbers followed by different letters are significantly different based on Duncan's Multiple Range Test. 3 ** indicates significance at p=0.05 ns indicates not significant at p=0.05. ALABAMAAGRICULTURAL ExPERIMENT STATION28 EFFECTS OF POULTRY LITTER ON THE YIELD AND QUALITY F STAKED T A VEGETES Edgar Vinson, Joseph Kemble, and Jeff Sibley Vast accumulations of poultry litter have prompted the search for beneficial and environmentally sound incorporation of this by-product into the fertility programs of vegetable grow- ers. To determine the effects of poultry litter on specific veg- etable crops, more research must be done. As a contribution to this multifaceted endeavor, a study was conducted at the E.V. Smith Research Center in Shorter, Ala- bama, in the spring of 1999 and 2000 to determine the effects of poultry litter on yield and quality of staked tomatoes. Quality attributes measured were citric acid content, soluble solids, total acidity, and the types of culls produced such as radial cracking, blossom end rot, and catfacing. Composted poultry litter was applied at rates of 30, 60, 120, 240, and 480 pounds of nitrogen per acre and incorporated into the soil with a roto-tiller prior to mulch bed formation. No methyl bromide was applied. 'Mt. Spring' tomato transplants were planted on these poultry-litter-amended beds. Yields of tomatoes grown in poultry litter were compared to the control treatment, which received 120 pounds of nitrogen per acre from a mixture of cal- cium nitrate and potassium nitrate (7-0-7) and was fumigated with 67% methyl bromide and 33% chloropicrin (see figure). In 1999, tomato fruit yields decreased with increasing amounts of poultry litter. Poultry litter applied at rates of 30 and 60 pounds per acre were sufficient for tomato production. In 2000 tomato yields were significantly higher in the 480 pounds-per- acre rate than in all other treatments. In this case, test plots were overcome by nutsedge so tomatoes grown in the heavily amended soils fared better because of a more abundant supply of nutri- ents for which to compete. The production of cull fruit such as blossom end rot, cracking, and cat-facing was similar in all treat- ments (see table). Total fruit acidity was significantly lower in the 480 pounds-per-acre treatment when compared to the control (see table). Total acidity, soluble solids, and citric acid 1 Marketable yield data for 1999 and 2000 were analyzed separately because of treatment and year interactions. 2 Columns headed by different letters represent means that are significantly different. 3 "Com-" =non-methyl bromide treated control and "com+" = methyl bromide treated control. content play a role in tomato flavor. Increased total acid content has been known to reduce flavor scores in taste tests. According to the 1999 experiment, poultry litter ap- plied at a rate of 30 pounds of nitrogen per acre is enough to produce yields above that of inorganic fertilizer without increasing the occurrence of culled fruit. A similar conclu- sion was reached in the 2000 study where tomatoes grown in poultry litter treatments pro- duced similar yields as toma- toes grown in with inorganic fertilizer. Physiochemical at- tributes of tomatoes grown in soils amended with poultry lit- ter at this rate are comparable to those grown using inor- ganic fertilizers. bt.p p p p 2001-2002 FRUIT AND VEGETABLE -RESEARCHREPORT 29 30 ALABAMA AGRICULTURAL EXPERIMENT STATION EFFECT OF VARYING AMOUNTS OF POULTRY LITTER ON CULLS OF 'MT. SPRING' TOMATOES, 20001 Poultry litter Blossom Cat- Citric Soluble treatment Crack end rot facing acid solids pH (lb/ac) (Ib/ac) (lb/ac) (%) (%) 30 Ibs N acre-' 362.0 a 4 144 a 2,957 a 0.37 bcd 4.5 a 4.1 a 60 Ibs N acre-' 784.0 a 673 a 4,526 a 0.34 cd 4.1 ab 4.1 a 120 Ibs N acre-' 663.0 a 1,251 a 3,259 a 0.40 b 3.9 b 4.1 a 240 Ibs N acre-' 1,026.0 a 1,251 a 6,457 a 0.40 b 4.1 ab 4.0 a 480 Ibs N acre-' 2,294.0 a 673 a 3,439 a 0.48 a 3.9 b 4.1 a Control- 2 302.0 a 144 a 3,379 a 0.39 cd 4.2 ab 4.2 a Control+ 3 1,328.0 a 385 a 4,523 a 0.33 d 4.4 a 4.2 a 1 Study was conducted at E.V. Smith Research Center in Shorter, Alabama. Results are in pounds per acre-'. 2 Non methyl bromide treated control. 3 Methyl bromide treated control. 4 Within columns, means followed by different letters are significantly different. -HIZOBACrERIAL-MEDIATED MATURE PLANT RESISTANCE IN TOMATO TO CUCUMBER MOSAIC VIRUS John F. Murphy, M.S. Reddy, and Joseph W. Kloepper The use of plant growth-promoting rhizobacteria (PGPR) to induce resistance to infection by Cucumber mosaic virus (CMV) in tomato has been investigated. Previous studies have showed that selected PGPR strains induced protection against CMV in tomato plants under greenhouse and field conditions and that PGPR treatments protected tomato plants from the white- fly-transmitted Tomato mottle virus (ToMoV) under field condi- tions consisting of heavy whitefly/ToMoV pressure. Mature plant resistance is an established but poorly un- derstood phenomenon whereby plants vary in their susceptibil- ity to infection by certain pathogens due to their age or stage of development. For example, in bell pepper plants the mechanism of mature plant resistance to CMV involves interference in pro- cesses that allow the virus to move throughout the plant. While previous studies focused on the use of PGPR to induce resis- tance against viruses, recent efforts have shifted to evaluate the enhanced plant growth effects that result from treatments con- sisting of combinations of PGPR formulated in chitosan. The purpose of this study was to determine whether enhanced plant growth may serve to shorten the window of time leading to ex- pression of mature plant resistance. Tomato 'Solar Set' seed was sown in Speedling trays un- der greenhouse conditions. Five PGPR preparations (termed LS series) were used in each experiment. Each LS preparation con- tained industrially formulated endospores of two Bacillus strains and the formulation carrier chitosan. Tomato seeds were sown directly into the LS/soilless growth medium mixture. Treatments consisted of five LS preparations and two non-bacterized con- trol treatments. One control treatment was the same age as plants in the LS treatments, while the second was 10 days older. Plants in the older control treatment were the same size as those in the LS treatments. Plants were mechanically inoculated with CMV when the same age control treatment plants were at the early 5 to 6 leaf stage. Three growth parameters were evaluated at the end of the experiment [30 days postinoculation (dpi)]: plant height (a mea- sure of stem growth from one day prior to CMV inoculation to 30 dpi), plant fresh weight of above ground tissues, and the number of flowers and fruit. The mean plant height was significantly greater for all LS treatments and the older control compared with the control treatment (Table 1). No differences were observed for TABLE 1. RESPONSE OF LS- AND MOCK-TREATED TOMATO PLANTS TO INOCULATION WITH CMV 1 Treatment Height Weight Flower/fruit (cm) (g) (no) LS254 26.4 b 123.5 cd 10.6 b LS255 26.5 b 130.0 d 13.6 cd LS256 26.5 b 119.5 cd 13.4 cd LS257 24.3 b 117.7 cd 13.7 cd LS213 26.9 b 114.5 cd 12.4 bc Control (old) 25.5 b 99.7 b 10.3 b Control 19.6 a 30.7 a 1.8 a 'Tomato plant response to Cucumber mosaic virus was mea- sured at 30 days post inoculation. 2001-2002 FRUIT AND VEGETABLE RESEARCH REPORT plant height among LS treatments and the older control treat- ment. The mean plant fresh weight was significantly greater for all LS treatments and the older control treatment compared with the control treatment. In addition, plant fresh weight for all LS treatments was significantly greater than for the older control treatment. The mean number of flowers and fruits was signifi- cantly greater for all LS treatments and the older control than for the control treatment. LS treatments 255,256, and 257 had signifi- cantly more flowers and fruit than plants in the LS254 and older control treatments. Initial signs of vein clearing and mosaic first occurred in control treatment plants by seven dpi, whereas plants in the other treatments were symptomless at that time. When plants were rated for symptom severity, the control treatment had a significantly higher average disease rating than for each of the other treatments at 14 and 28 dpi, whereas the LS and older con- trol treatments did not differ from one another. More than half of the plants in the LS254, LS255, LS213 and older control treat- ments and half of the plants in LS256 remained symptomless at 28 dpi. CMV accumulation in young, uninoculated leaves was measured at 14 and 28 dpi by ELISA. At 14 dpi, the mean ELISA value for samples collected from each LS and the older control 31 treatment was significantly lower than for the control treatment (Table 2). CMV accumulation in plants treated with LS213 was significantly lower than in plants treated with LS257 but neither treatment differed from the other PGPR treatments or the older control. When plants were tested at 28 dpi, mean ELISA values for treatments LS254, LS255, LS256, LS213, and the older control were significantly lower than for LS257 and the control treat- ment. ELISA data, used to determine percent infection within each treatment, revealed that significantly fewer plants were in- fected with CMV in the PGPR treatments LS255, LS213, and the older control than in the control treatment at 14 dpi (Table 2). At 28 dpi, treatments LS254, LS255, LS213, and the older control had significantly fewer infected plants than in the control treatment. In addition, the percentage of infected plants was significantly lower for treatments LS254, LS213, and the older control than for LS257. Since mature plant resistance has been used as a manage- ment tool to reduce virus infection and associated yield losses under field conditions, the LS-based treatments reported here may offer a form of PGPR-mediated induced mature plant resis- tance that would integrate well with other pest management schemes. TABLE 2. DETECTION OF CMV IN LEAF TISSUES OF PGPR-TREATED AND NON-TREATED TOMATO PLANTS' Treatment ELISA- -Percent infection 3- 14 dpi 28 dpi 14 dpi 28 dpi LS254 0.496 bc 0.328 b 40 abc 30 c LS255 0.362 bc 0.455 b 35 c 55 bc LS256 0.633 bc 0.409 b 60 abc 60 abc LS257 0.697 b 0.715 a 65 abc 85 ab LS213 0.318 cd 0.336 b 25 c 30 c Control (old) 0.423 bc 0.332 b 35 c 35 c Control 1.228 a 0.774 a 90 ab 90 a 'Cucumber mosaic virus was detected by enzyme-linked immunosorbent assay (ELISA) at 14 and 28 days postinoculation. 2 Mean ELISA values for CMV-inoculated tomato plants subjected to the stated treatments. The numbers represent the mean ELISA value for all (20) plants per treatment. Different letters represent a significant difference of the means at P-0.05. 3 Percent infection is based on the number of samples shown to be infected with CMV based on ELISA per 20 samples for each treatment. Different letters represent a significant difference of the means at P-0.05. ALABAMA AGRICULTURAL EXPERIMENT STATION EVALUATION OF CUPROFI MZ DISPERSS FOR THE CONTROL OF CERCOSPORA LEAF SPOT ON TOIMATO, 2002 Edward Sikora and Arnold Caylor Cuprofix MZ Disperss is a new product from Cerexagri. It is a fungicide/bactericide that combines the active ingredients cop- per and mancozeb. Mancozeb is effective in controlling fungal diseases such as early blight, whereas copper acts as both a fungi- cide and a bactericide and is effective against bacterial leaf spot. This trial was conducted to determine the efficacy of Cuprofix MZ Disperss against Cercospora leaf spot on tomato. Cercospora leaf spot is often a problem on fall-grown tomatoes in Alabama. Results indicate that the 4- and 6-pound-per-acre rates of Cuprofix MZ Disperss applied weekly were effective in controlling the disease. This trial was conducted at the North Alabama Horticul- tural Research Center in Cullman, Alabama. Tomatoes were trans- planted in July of 2002. Plants were grown on bare ground and drip irrigated. The experiment consisted of five treatments, repli- cated five times, in a randomized complete block design. Each treatment/replication consisted of a one row plot bordered on each side by an unsprayed guard row. Rows were 25 feet long with an in-row spacing of 18 inches. Treatments were applied weekly. Assessment of disease was conducted on October 12 and October 23. Yield data were not available at the time of pub- lication. EFFECTIVENESS OF TREATMENTS ON CERCOSPORA LEAF SPOT ON TOMATO Treatments Percent Cercospora leaf spot rate/acre October 12 October 23 Untreated control 14.0 69.2 Cuprofix MZ Disperss 4.0 Ib 2.6 12.3 Cuprofix MZ Disperss 6.0 lb 2.0 8.6 Cuprofix MZ Disperss 4.0 lb 10.4 22.2 ALTERNATED weekly with Actigard 1/3 - 3/4 oz + Cuprofix MZ Disperss 2.0 Ib ManKocide 5.0 Ib 16.0 60.6 The 4- and 6-pound rates ofCuprofix MZ Disperss had the lowest Cercospora leaf spot ratings on both rating dates (see table). It appears the 2-pound rate of Cuprofix MZ Disperss in the alternating treatment was too low to control Cercospora leaf spot. ManKocide performed poorly with disease ratings in the same range as the untreated control. IR-4 FOOD-USE RESEARCH ON TURNIP -GREENS Edward Sikora and Arnold Caylor Alabama is the second leading producer of turnip greens in the United States with more than 2,000 acres planted annually with an average yield estimated at 11,000 pounds per acre. The crop is valued at more than $5 million in Alabama. Production of turnip greens can be affected by a variety of plant diseases. Damage can range from minor spotting to complete loss of a crop depending on the pathogen involved. Because leaves are typi- cally the marketable products in green production in Alabama, a minor leaf spot problem can cause a significant loss in yield. Only a few fungicides are labeled for use on turnip greens inAlabama. For this reason, funding from the IR-4 program was used to evaluate non-labeled and new chemistry fungicides for control of foliar diseases of turnip. Results indicated that two rates of Quadris F and BAS 500 (an experimental product from BASF) reduced damage from Alternaria leaf spot compared to an unsprayed control on the variety 'Purple Top'. These experiments were conducted at the North Alabama Horticultural Research Center in Cullman in 2001. The turnip varieties 'Purple Top' and 'Shogun' were transplanted in Sep- EVALUATION OF FUNGICIDES FOR CONTROL OF ALTERNARIA LEAF SPOT ON TURNIP GREENS, 2001 Treatments/ -Percent Alternaria leaf spot- rate/acre Shogun Purple Top Untreated 4.2 a 1 14.2 a BAS 500 (1 Ib/acre) 4.7 a 7.0 b BAS 516 (0.66 Ib/acre) 4.5 a 10.0 a Quadris F (142 mI/acre) 3.7 a 7.2 b Quadris F (237 ml/acre) 3.5 a 6.2 b SNumbers followed by the same letter are significantly differ- ent. tember into separate trials. Each experiment consisted of five treatments replicated four times in a randomized complete block design. Plants were grown in one-row plots that were 15 feet long. Treatments included (1) an unsprayed control, (2) BAS 500 (1 pound per acre), (3) BAS 516 (0.66 pound per acre), (4) Quadris F (142 milliliters per acre), and (5) Quadris F (237 milliliters per 32 2001-2002 FRUIT AND VEGETABLE RESEARCH REPORT acre). Fungicide applications were initiated at the first sign of disease and a total of three applications were made at 14-day intervals. Disease ratings were taken in late November and mar- ketable top and root weights were determined at harvest. Alternaria leaf spot, a common fungal disease of turnips, was the most common disease detected in these trials (see table). Disease pressure was relatively low in the 'Shogun' trial appar- ently due to the variety's higher level of resistance to the dis- ease. There were no significant differences in disease ratings among the fungicides and the unsprayed control in the 'Shogun' trial. Damage from Alternaria leaf spot was significantly higher on the unsprayed control and in the BAS 516 treatment com- pared to the other three fungicide treatments in the 'Purple Top' trial. There were no significant differences in yield (top or root weights) among the treatments in either trial (data not shown). 33 Alabama's Agricultural Experiment Station Auburn University Research Unit identification ) Main Agricultural Experiment Station, Auburn. 'k Alabama A&M University r E. V. Smith Research Center, Shorter. 1. Tennessee Valley Research and Extension Center, Belle Mina. 2. Sand Mountain Research and Extension Center, Crossville. 3. North Alabama Horticulture Research Station, Cullman. 4. Upper Coastal Plain Agricultural Research Station, Winfield. 5. Chilton Research and Extension Center, Clanton. 6. Piedmont Substation, Camp Hill. 7. Prattville Agricultural Research Unit, Prattville. 8. Black Belt Research and Extension Center, Marion Junction. 9. Lower Coastal Plain Substation, Camden. 10. Monroeville Agricultural Research Unit, Monroeville. 11. Wiregrass Research and Extension Center, Headland. 12. Brewton Agricultural Research Unit, Brewton. 13. Ornamental Horticulture Research Center, Spring Hill. 14. Gulf Coast Research and Extension Center, Fairhope.