High-fidelity long-read assembly of the feline gut microbiome

Abstract

Long-read reference contig assembly of domestic cat rectum/fecal microbiome. DNA was extracted from fecal samples collected from client-owned domestic cats using Qiagen Allprep PowerFecal DNA/RNA kit. PacBio sequencing library was constructed and sequenced on a Sequel II machine. Microbial contigs were assembled using hifiasm-meta, and contig binning was performed using PacBio pb-metagenomics-tools pipeline.