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Two-photon imaging of Zn2+ dynamics in mossy fiber boutons of adult hippocampal slices

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dc.contributorChristian R Goldsmith, crg0005@auburn.eduen_US
dc.creatorKhan, Mustafa
dc.creatorGoldsmith, Christian R.
dc.creatorHuang, Zhen
dc.creatorGeorgiou, John
dc.creatorLuyben, Thomas T.
dc.creatorRoder, John C.
dc.creatorLippard, Stephen J.
dc.creatorOkamoto, Kenichi
dc.description.abstractMossy fiber termini in the hippocampus accumulate Zn2+, which is released with glutamate from synaptic vesicles upon neural excitation. Understanding the spatiotemporal regulation of mobile Zn2+ at the synaptic level is challenging owing to the difficulty of visualizing Zn2+ at individual synapses. Here we describe the use of zinc-responsive fluorescent probes together with two-photon microscopy to image Zn2+ dynamics mediated by NMDA receptor-dependent long-term potentiation induction at single mossy fiber termini of dentate gyrus neurons in adult mouse hippocampal slices. The membrane-impermeant fluorescent Zn2+ probe, 6-CO2H-ZAP4, was loaded into presynaptic vesicles in hippocampal mossy fiber termini upon KCl-induced depolarization, which triggers subsequent endocytosis and vesicular restoration. Local tetanic stimulation decreased the Zn2+ signal observed at individual presynaptic sites, indicating release of the Zn2+ from vesicles in synaptic potentiation. This synapse-level two-photon Zn2+ imaging method enables monitoring of presynaptic Zn2+ dynamics for improving the understanding of physiological roles of mobile Zn2+ in regular and aberrant neurologic function.en_US
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen_US
dc.subjectzinc ion metalloneurochemistryen_US
dc.titleTwo-photon imaging of Zn2+ dynamics in mossy fiber boutons of adult hippocampal slicesen_US
dc.type.genreJournal Article, Academic Journalen_US

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