2?- t. I. -~ - U, gt*- 1 Alabama Agricultural Experiment Station 1995 annual r, i Alar _.A r z- 491& CONTENTS Introduction Food and Sport Animals Plant Production Integrated Pest Management Food Safety, Quality and Nutrition Environmental Quality Economic, Social and Health Issues 1995 Director's Research Awards Financial Report ALABAMA AGRICULTURAL EXPERIMENT STATION Administrative Officers William V. Muse, President Paul E Parks, Provost and Vice President for Academic Affairs C. Michael Moriarty, Associate Provost and Vice President for Research Lowell T. Frobish, Director David H. Teem, Associate Director Russell B. Muntifering, Associate Director Patrick D. Green, Assistant Director James R. Roberson, Assistant Director The 1995 Annual Report was produced by the Office of Research Information: James R. Roberson, Assistant Director/Editor Catherine L. Smith, Associate Editor Robert A. Hearn, Associate Editor Teresa E. Rodriguez, Art Designer For more information about the contents of this publication, call (334) 844-4877. The information contained herein is available to all, regardless of race, color, sex or national origin. aGRICULTURAL research: An INVESTMENT in the x LIRE iike rowing upstream: not ;c advance is to drop back," (Jescribes the research progru or the Alabama Agiicultura! Experiment Station (AAES). G iesearch must addess not on today' pobeu~ Production of food and fiber is no longer an island unto itself, but is influ- enced or affected by many factors. G?o\vernmnental programs, woraid trade poli- cies, envirorrnrenta concerns and con- sumer perceptions, to name a few, greatly impact production efficienc. A particular chemical may control a specific weed, insect, or plant or animal disease but its impact on the environment must be ascer- tained. Hence, our research must verify that existing production practices are still viable, economical and environmentally safe. Integration of the various manage- ment practices into a total production system is needed. Development ot a total production sys- tem requires use of existing practices as wX ell as the investigation of new ideas. The research described in the 1995 AAES \nnual Report represents a small portion of our total research program, but it serves to convey the scope of our efforts to move Alabama into the future. From basic to applied science, AAES researchers took huge steps forward last year in efforts to improve crop and live- stock management, develop new agricultur- al products, protect the environment, sate guard against foodborne disease and con- front economic and social issues vital in ensuring the well-being of Alabama's citi- zens. For example, Auburn's active biologi- cal control research program discovered beneficial microorganisms that control many common agricultural pests, reducing the need for chemical pesticides. Genetic engineers developed tobacco plants that produce a material which can be used to make biodegradable plastic. Animal scien- tists devised a technique for using ultrasound to produce leaner pigs. Aquaculturists bred a hybrid catfish that could earn millions in new revenues for southeastern tish farmers. Poultry scientists formulated disintectants that coumld better control Salninella bacte- ria attached to chicken carcasses. Entomologists developed a pest control strategy that suppresses cockroaches taster, better and longer than conventional treat- ments, but uses much less pesticide. Consumer affairs researchers put Alabama's textile and apparel manutacturing plants on the Information Superhighwa, giving this industry a much-needed competitive boost. These are but a fewv examples of the diverse accomplishments Iade by the AAES in 1995, but they exemplift the goals all our scientists strive to achieve. Investing in research is like a savings account. If one wvaits to save until the money is needed, it may be too late. Research conducted today wXill ensure that the production and utilization of food and tiber will remain an important contributor to the economy of Alabama. Lowell T. Frobish AAES Director AAES ANNUAL REPORT I foo( and SPORT A labama is blessed with diverse anin. agriculture industries, including bee poultry, fish, swine and dairy produc tion. Also, the state's abundant nat.. resources give us economically imp tant game animals, such as white-r' deer and largemouth bass.T ty of animals requires a dive research program to ensure management and continued growth- crelated industries. AAES scientists addressing wide-ranging issues imp tant to these segments of th economy: animal health, new improved animal products a' effic While most southeastern livestock producers hav e not yet heard of the para- site Neospora caninum, it could be a hidden cause of abortions and calf deaths in the region. AAES animal health identified the parasite in Alabama cowXs and dceveloped a vaccine to treat the disease. There are no statistics on the parasite's economic dam- age in the South, but it is known to have an impact of more than $35 million in California. Researchers tested laboratory strains of the parasite for tse as vaccines. Researchers altered the parasites so that they no longer cause the disease, but rather build up treated animals' immunity. Preliminary evidence indicates that the vaccine protects against the organism. AU scientists are working with a pharmaceuti- cal company to test the vaccines and experiment with adjuvant chemicals to boost the effectiveness of the treatments. Infective cysts of the parasite are spread in wvild animal wastes, but the precise epi- demiology is still under investigation. In other animal health research, sci- entists began testing in January 1996 on SVt Mn AAES p b l c d pl es ' arc o A -+ f ' - F humans ha livesto Crptidu life-threatening complications in human AIDS patients. It is likely that the new treatments will soon be used in clinical AIDS studies. AAES ANNUAL REPORT-2 AWbl bbtV .d91 V 4 Ludies ranging from deveioping new treatments for parasitic infections to increasing the value of cuii cows to overcoming the effects of toxic fescue. AAES animal scientists used real- time ultrasound in a selective breeding pro- gram to develop pigs that produce more lean pork with 34 percent less feed. Their technique can be used easily by swine breeders in the field. Ultrasound was used to identify low- backfat pigs for use in selective breeding. Using ultrasound data in combination with growth efficiency and growth rate measure- ments, researchers developed an index that predicts feed efficiency. This index allows breeders to use ultrasound and growth rate data to select more efficient animals, thus removing the need for costly and difficult feed conversion tests. Researchers have completed three years of research on a purebred Duroc line using this technique. This selected line has 18 percent less backfat, 13 percent larger loin eye area, 31 percent greater daily lean growth and 34 percent better lean feed efficiency. A s with much lean pork, the meat is less firm, has higher water loss and is less flavorful, but these problems are not as severe as they are in most other lean lines. Also, unlike many other lean lines, these Durocs do not have the stress gene, which is related to poor meat quality and sudden animal death. Animal scientists now plan to use ultrasound in an effort to identify g id AAES ANNUAL REPORT 3 ~;riE i 1 ~--f o ; ;.iY tzi Animal health researchers are using pygmy goats to test a new vaccine against a parasite that could be causing abortions and calf deaths in Alabama cattle herds. muscle quality in live pigs. Researchers also will seek genetic markers for higher-quality muscle. The goal is to use this information in selective breeding to eliminate problems with firmness, flavor and water loss in lean pigs. An AAES-developed hybrid catfish has the potential to increase the Southeast's $2 billion catfish industry by at least 20 percent. After more than 20 years of research, the first large-scale attempt to produce these hybrids will occur in spring 19906. A cross between female channel cat- fih and male blue catfish, the hybrids grow at least 20 percent faster than channel cat- fish. They are more resistant to oxygen depletion and to columnaris, enteric sep- ticemia and other diseases. And they are easier to remove from ponds by seining or angling. Overall survival is about 30 per- cent better in the hybrid fish. These bene- fit> are greater in the higher stocking densi- ties and more stressful growing environ- ments common to commercial catfish pro- duction. In addition, the hybrids yield about 1-4 percent more edible flesh. Blue catfish and channel catfish do not mate naturally, so a major hurdle was dc\eloping artificial fertilization techniques to hatch enough hybrid fingerlings for com- mercial production. Another barrier is achieving cost effectiveness with a highly labor-intensive breeding system. Breeding methods developed at Auburn proved not only to be competitive, but to provide increased profits for the catfish industry. Researchers found that hybrid finger- lings can be produced at the same price as channel catfish. However, fingerling pro- ducers can almost double the price for hybrids, and farmers can still make a 10 percent better profit. Poor-quality or old brood cows retired from a herd are byproducts of cow-calf pro- duction; 10-15 percent of Alabama's 920,000 brood cows are culled and sold each year. These cows, which often have poor muscle quality, are now shipped away immediately after culling for use as raw material in ground meat or other low-grade products. However, AAES animal scientists are developing techniques to enhance the value of cull cows. One goal is to use low- cost extra management to add muscle mass without adding fat to the cows. Management strategies include feed- ing the culled cows for 60-100 days while using growth-promoting agents to enhance the animals' muscle quality. Scientists demonstrated that treating cull cows with the genetically engineered growth hormone bovine somatotropin (bST) can increase muscling and feed con- version by about 40 percent. The synthetic AAES ANNUAL REPORT--4 testosterone trenbolone acetate (TBA) increased muscling by 20 percent and feed conversion by 40 percent. Researchers are now investigating the potential for combi- nation effects with TBA and bST. Basic information uncovered by AAES animal scientists could help produc- ers counter the harmful effects of cattle feeding on endophyte-infected fescue. Endophytes, fungi that grow between plant cell walls, decrease cattle pregnancy rates and calf growth rates. Animal scientists found that cows grazing infected fescue actually have a nor- mal 21-day estrous cycle and do become pregnant. However, when the animals are examined 42 days into pregnancy, several will no longer be pregnant. Blood samples indicated that this is not a reproductive hormone problem. To explore this mystery, researchers bred cows not fed fescue, flushed out healthy embryos and implanted them in heifers that were grazing on infected fescue. Whether the animals were bred or implant- ed, the rate of pregnancy was still reduced at 42 days. Scientists are now testing the theory that the toxic fungus somehow interrupts the biochemical process that signals to a cow's body that it is pregnant. A fetus must biochemically communicate to the uterus that it is there, and the uterus must recog- nize this signal so the cow's body can respond appropriately. If these events do not occur, the ovaries begin to produce new eggs and the fetus dies. In a spring 1996 grazing trial, researchers will use mol- ecular techniques to examine embryo and uterus tissue samples. The goal is to mea- sure whether the proper biochemical events actually take place. AAES aquaculture researchers in October 1995 harvested the first crop ot pond-raised shrimp in a project to develop culture technology for this commercially important shellfish in Alabama. Goals included evaluating the growth and culture of the native Gulf white shrimp in South Alabama and assessing the economic, ,f its production as a bait or food fish. Preliminary results indicated that it i, highly profitable to grow these native shrimp as bait. Gulf white shrimp produced 4,000 pounds of bait per acre. High stock- ing density proved to be most profitable in bait production. Researchers will stock at even higher rates in May 1996 and focus on studies of aeration, management effi- ciency and water quality. In the proposed long-term project, decades of Auburn cat- fish research will be adapted to the produc- tion of shrimp in brackish ponds. Since annual shrimp harvests are sub- ject to drastic seasonal variability, many producer, in Mobile and Baldwin counties A new AAES-produced hybrid catfish could increase the South's cat- fish industry by 20 percent. AAES ANNUAL REPORT - 5 =p~ P--i Ilrc"'I/ ~4a, a are interested in pond production of the shellfish. However, many basic questions must be ansvered betore wide-scale produc- tion is advisable. Bait production appears to he iich more protitable thin toOd tsh production. Bait shrimp which are about one-third as big as mlediul food shrimp at market size sell for almost three times as much as tood shiimp. The native Culft white shrimp is superior for bait production. This research is condliCted at the Alabama Department of Marine Resources facility in Culf Shores. In a companion study, the Cult Coast Research Laboratory in (c ean Springs, Miss., is working on shr imp hatchery technology. p ," Poultry scientists are focusing on nuil- tiple aspects of production, transportation and processing that affect final poultry prodtict quality. From the feet to the breast, these studies have yielded information that increases profits tor the poultry industry and provides higher quality products tor conSiincrs. In one study, iesearchers found that AAES ANNUAL REPORT- 6 long transportation distances, especially during high temperatures, can significantly reduce the yield of sellable prodlict. Chickens tend to shrink about .25 percent per hour inder opti mum transportation conditions; double that in hot weather. However, scientists determined that pro- viding elccttroly tes and Vitamin C in water betore slaughter can reduce this shrinkage. Responding to an export demand for chicken feet, researchers are examining environmental and dietary conditions that reduce the qtuality of a chicken part once sold to rendering plants for about 2 cents per pound. In Asian markets, chicken paws can be sold for about 50 cents per pound. I ligh ammonia concentration in chicken house litter can cause dermatitis in the feet, creating sores that make them unsal- able. Researchers found that increasing the concentration of dietary zinc enhances healing. Poultry scientists also are evaluat- ing sand as an alternative bedding material to reduce chicken foot lesions. Other studies focus on skin quality in whole birds. In one project, researchers tested a feed additive to control the proto- zoan disease coccidiosis. They found that -;-a- - - it ..A AAES aquaculture researchers, in cooperation with t develop culture technology for this important shellfi! : i~l Fem.Cp 41 Lt ~l~4ir '~%* -I -- ~fl~- -4 1*~' F, w p ~ N< abama Department of Marine Resources, harvested the first crap of pond-raised shrimp in a project to 77 r "A Y a tlw aJltit ix C hafltpci S. t11aI ~Igt'ntilh()- lkm, al problcll that CrdUCS Sk4.inl tpilit x il l srenth. Testscn ih.mt srrait tihati -~ the atlJlttix e Shoiild not he uised ill ill dlfring thC first three wveeks of pro li- dioll or- IIbd Ctt oI tIlol' inl Stibs'.eien N' eeL. an,(lers n~lilli ll [i~ hilt Jenlle ailjiitic Alihalia rescr\v oii', but .AA P f isheries it re( ilrh has Shownl thatl this is nut the in itinlalt fait t r inl I he rirot tx\' e it f ~ e'.art her'. fundl liii icenCi ionl i inic of a like'. v atc cin the s.pirigt iii] cirN suiimnCr is. more importa.nt. Deiteniion tinfl, the rit e at w~hich wilier is. fhisliet fitraalake, lange'. from tw~o to 41 l9 ays ON Il Alabma ricr.~i0ii. Too rafipi wltc P lrn'ienlt Paus'es. algae iti [mitroscopic ;1ittiills to oct flooded (ill of a1 re'.tmxoil, tilt itely iedlutiiig theltitotii of prcy foir biass intl other '.pe it'. ,t itl1I d' del ltnc that a i et en- AAES ANNUAL REPORT-7 AAES fisheries researcher s found that the rate at which water flows through Alabama's reservoirs can have a huge impact on bass and crappie populations. to enough algae and plankton develop- to catc mieat to suipport a t ng food base. more 1 At Lake Guntersville, tot example, water volume turns over every 12 days on A asverage. Under rainy conditions, when the scored lake flushes faster, bass reproduction is the die reduced. During dry periods water retention Deer n is 20-25 days, and there is hettei reproduc- tein ye tion. In Weiss Lake, resource managers tial, hr keep the water level as high as possible only 7. between April and June, providing a greater young bass poipultion. Plants were scienti tound to help increase bass populations in 22 per last-flushing lakes. only I Plants c apture nutrients and provide a seen ir food souircLe for young bass. lnfrunately, anial 'rovthis low inl young fis 1 ) I tihat rely on dense vegetation, and many do not reach a large enough size to survive the win- ier. Researchers found that plaint coverage of more than 15 percent of a lake's surface caus- es a reduction in adult fish growth. Anglers are moie likely h a greater number of small bass in eavily planted lakes. AAES wildlife research has under- the importance of crude protein in is of white-tailed deer in Alabama. eed at least 17 percent dietary pro- ar-round to maximize growth po)ten- It native vegetation often provides -11 percent. Jsing AU's captive deer herd, wildlife sts fed one group of fawns a diet with cent crude protein and another group 1 percent. Dramatic differences were a all measured characteristics of the s. For example, yearling males fed the higher-protein Jiet were 14 percent heavier and had antlers 52 percent heavier. To help land managers economical ly provide year-round, high-protein forages for w ild deer, researchers also determined the Ilk )st cost-effective mixture of 39 cool-sea- a rn and nine warm-season grains, legumes and grasses. Researchers found wheat, oats and rye to he the most cost effective and most pro- dictive ciool-season torages from September-March, costing less than $100 per ton of forage. If they are planted on good soils that support reseeding and perennial growth, Crimson clover and rye- grass are cost effective from winter through early spring, costing less than $200 per ton of forage. Combining these grasses and small grains supplies abundant, high-uality forage during a stressful period for deer, and it also attracts game to forage plots during hunting season. Red clover and ladino clover, wvhich cost less than $50 per ton of feed, are very cost effective from April to September. Red clover - and ladino, if it is planted on high- uality sites contin- ues to produce well in the summer, when native vegetation is scarce and low quality. Soybeans, velvetbean and peas also are cost-effective warm-season forages, costing less than $60 per ton of forage. AAES ANNUAL REPORT -8 4.. Auburn's Turfgrass Research Unit plays a major role in AAES plant piroduction research with studies designed to improve care and golf course management in the South. plain. systemns I eseal tfli A Es as varied as the plant AAEspecies that play such vital roles in the state's economy. Innovative techniques to improve the value of row crops and to develop hardier grasses foi golf greens, disease-resistant ornament~li plants for landscape use and productive new forages for livestock production arec but a few of the goals Aubur n iresear cheirs are stiingi to atairn. CoUt s- Sounthern "Olt courses coU I enjory .oils idcrfflL SnIv'ings [i itiurgeiflet expenses becatise of AAES t trtgra', reset1rch. Agronoitst tiec ex .thitt ing Berinutidgrals (tilt ixti- M"In c~ot 'pe' that dexveloped nit- nirly tin }greens throutghot the region titJ 11i;1 be bet- ter n1dnpted to hot, h uid summniers in cold, rainy winters. PRODUCTION ReIV ,CIfhet' ll I'i plineJ (Iiiu experiieitd .l ines .itt eight sceect e Tilgreen ,inJ law~n I ildxx~lil x, ii iit onuICe tell lr-t111 Viu hioi','iiies, inctlding seiet t gnlf C out se gireen,. The iii Igi sse ire being ev;diuiCit on ;1f .i 1.S. CGit.\',tt A ollonttc t utting green Aind on fitixe 'Hii1i\ loinm soil. In 1996, ilgiino- mists wxiii suiject the test t oturses ton xii 10th mlh ting heights mid t opdress treylnils, wxhile sinnniit ing the stress ot tralttict i lexel si mi ir to i"At, course pla1y. Plinintiy re'.eii it showsed 111hit grrxx in of graiss icover xx is sioxvet 1on the USG A green, inicit i rg t1,1 unit i nK seiected Ironm locii putt rug greeits in;iy he AAES ANNUAL REPORT -9 moie adapted to natixe soil. Also, spring Seedhead prouductnin, xxh ith sexerely iiiipait s tui- o lial it y, xxas un ore of a pr ibleim onl the USG A gireen. Hoxxever, the grasses xxere usually darker on the 1. SC A green, ilkd sp ring green tp ratings wxere higher o that green. A Titdxxarl vairiant from at Movlc I go lf t ourse wxas gireener than mttoI other grasses, but it xxas the only gras, i produce tall secdhcads. A variant tromi Lakexxood produced morue spring seedl id, than mos t if the othei glasses, while the Mobile vaiaint did not produce spring seed heads. AAES micro biologists haive genetit .1- lengineeired t bhatco plants t hit prodluce poblyester, a protein-bised po lymer that . an be used to mtake biodegradable plastic. This bireaikthrouigh wa~s the tiirst time Scientists halvxe dcmn striii d the expression of a syn- het it gene in plints. Piroductiton of petrioleuii-bVsed plas- ics requiries iM-aidotis chemicails, and dis- po sal it these piodutc ts presents mta] u solid waste problems Proitemn-based poldymers ire enx iininut iil sate, cans be made fromt renewa'ble tesottts and break dowxn natt mally dittc disposal. In iddit ion to use in plastic patckagmng, the polyimeris ian be used in imedic ii supplies, controlled-release agro- chietmical productts and other iiidistriaml ippl icat ions. I lo wexer, tor pr otein-based polym ets to comipete xxith petr ileuim-based Genetically engineeired tobacco could provide an environmentally safe source of plastic. plastics, they must be produiced abundant ly and inexpensively. One ot the most pro- duictixe and economuical ways to pmroduce the poly mer is to hatrvest it fiom plants. Scientists first experimented xvith iso- lit ing a natural polymer-related gene from bacteria that prodit e small amounts ot poly~ester, but this gene prov'ed too complex tomanipulate Researchers at Auburn and the University ot Alabama at Birmingham then created a sy nt hetic gene xxith similar properties but with a simpler genetic struturae. AU microbiologists used a gene gun to insert this gene into tlbacco chhloplists. In this process, tungsten partic les xvere coated wvith Ithe synthetic DNA and accelerated into plant tissue by a gunpowder I targe. These tiSSUe samples were cull- t Uiied and groxwn into mature tobacco I plants, wxhich were shown to express the sy nthetic gene Researchers are nowx try ing to determine whether these parent plants transter the gene A o Subseq~uent gnrtos AAES h irticulturists have a bodprogram of investigating the best ornamsentalI plants foit Alabama. UResearchers continued to evaluate red mtaples, shade trees, crape my~rtles and tlower ing dogwvoods, and in 1996, a rhododendion study~ will begin. In a comparison ot red maple cultivars at the Piedmont Substation, Autumnt Elamne, Autumn Blaze, Fairviewx Flame and October Glory xxere found to be the best tot the Southeast, based on groxwth and tall color. In a test of crapemyrtle eliltivars, toliage of several selections was tree of poxx- dery mildew: Acoma, C addo, (Cherokee, Choctaw, Comanche, N athez, Osage and Sarah's Favorite. However, several mildew- tree oi resistant ctiltixvars Acoma, AAES ANNUAL REPORT 10 C omanche, Near East and Yuma exhib- ted extensiv e Ccrcoslvna leaf spot andI somte Piremature leaf shed. (irbin'.s Adk ins, C arolina Beauty, Wonderful Whbite and Raspberry Sundae were suisteptible to both diseases. OnlyiI light h rliaii spo)ttinig was seen on Fantasy, Tusc arora and Tuskegee, all of which also had excellent mildew resistance. In flowering dogwoods trials, little or no pow~dery mildew damage was seen in the (Jiant D~ogw~ood; the lKorean dogwoods Mlilky WXay, M/ilky WXay Select and Satomi; or the Corus kousa x Florridai hybrids Stellar Pink and Aurora. Among the tradi- tional flowering dogwoods, only Chlerokee Brave was tree ot mildew. Heaviest mildew damage was seen the common dogwoods Autumn Gold, Pink Beauty, Pink Flame, and Wonderberry, and First Lady. Most selections, with the exception of the ctiltivar Rainbow, showed few signs of anthracnose. Managing Native Forages Alahaima's Black Belt needs alterna- tive warm-season forage grasses; c immon ly used species such as Bermuda and Bahia are not well suited tothis region. However, native perennial grasses are naturally adapt- ed to the state's growing conditions and could meet the needs of Alabama farmers. AAES agronomists are develoiping strate- gies to manage these grasses for torage pro- duction. Researchers planted several nativ'e bunch grasses big bluestem, Indian grass, switc hgi ass, little bluiestem and Eastern gamagrass -in Black Belt pastures in spring I1995. Goals inclhide determining fatoi rs critic al to succ essfuil estibl isl lin t of the grasses: temperature, moisture, feril-I ity, date and method of planting and other factor s. Agronomists also hope to deter- mine how muc h graz-ing the grasses can tol- crate, how often and how shoirt they can be c ut for hay and how~ i he intflient e sedi- ment and nutrient loss from Pastures. In a~ddit ion, rhe pr oet iwill seek ro idountify adv antages and disadvantages ot integiriting native forage-livestock produittion into major Alabama cropping systems. Unlike the sod-itype grasses c ommonly used as for-ages, soil comfpac tionW is inot a pirobleim for many native grasses. Compaction, caused by vehicle and cat ilo t a f c d e l t s oi o x g n a d c n pove n t P la n ts fro m t re ai ch in g w a te r a n d n u t i - ents. Switc hgrass and Fastern gamagrass, for example, cin penetrate compac ted sil by rooting through the restrictive layer in order to reac h deeper moisture and nut ri- ents. Even in diought, the deeply rooted switc hgrass can thrive. Nat i\r claIsses ( i also enhami. e wildlife habitat. 0~ focus of AAES horticulture studies. AAES ANNUAL REPORT I I INTEGRATED management j F Alba, A4 giricultut al Ptroduction on the level required to meet soci- ety's needs is not possible ts d without the use of advanced technology and tactics. Unfortunately, as the demands on agriculture increase, the ?iaditional arsenal against crop f liseases, weeds and insects is ecoming more and more lim- ed. Environmental concerns lave put tight constraints on ising the chemical pesticides h fat made modern agriculture ossible. Researchers in the \AES are developing or evalu- Ling an array of environmen- Illy safe new weapons that ould ensure continued Several AAES integrated pest management projects benefit King Cotton, one of Alabama's top cash crop. gr owth in agriculture. AAES ANNUAL REPORT 12 Genetically Engineered Cotton Studies Genetically engineered crops are an increasingly important weapon in the arse- nal against agricuturai Ipests. H lowever, these weapons must undergo caretul testing before they can he tsed on a large scale. AAES researchers are testing three new genetically engineered cotton varieties to mnaximize the economic benefit of these crops and determine how they can best be integrated into management strategies. Two of the new prodicts ire geneti- cally engineered L)eltapine cotton varieties fromn Monsanto - Pnllgard and Roundup- ReAdy (otton. Boilgard contains Bacillus thuringienisis (Bt) genes that ena"ble the plant to produce proteins toxie to the insecticide-resistant budwormns that have devastated much of Alabama's cotton. Roundup-Ready is tolerant to the broad- spectrum herbicide Roundup. In studies at the Prattville Experiment Field, researchers found that Bollgard is 99 percent effective against the budworm. Bo llgard seed should be avai table in 1 996, and Alabama farmers lire expected to plant more than 60 percent of their cotton fields in the genetically engineered crop. It is ditfic ult to apply herbicides to normal young cotton plants withoult dam- aging the ciop. Researchers found that multiple applications of Roundup can be harmlessly applied over the to p of youn,_, Roundup-Ready cotton to control a w ii spectruim of bioadleaf- weeds. Undet lu ux- rait'fll condi- tions, which hindered acti- \'ation of pre- emergence herbicides, Roundup pro- vided efferl weed col , Hoe\'ev resealrch showed tii applic ations ImPst be test iiu.led to ~- l the season. Roundup-Ready (Cotton was registered for use in 1995. Auburn researchers have also studited L>; 4e1A'IL N SpeciaI colpu1tel-assslsSc Camera equipment (left) was used to study how genetically engineered, bioluminescent pathogens grow inside cab- bage plants. The photo above shows a diseased cabbage leaf, while the photo below pinpoints disease-causing bacteria in the same leaf, even in areas where there are no outward symptoms. AAES ANNUAL REPORT 13 the use of BXN Cotton, a Stoneville vari- ety from Calgene that is immune to the herbicide Buctril. Researchers fLind that BIuctril can be safely sprayed over young BXN cottonl to proxvide effective control of broadleaf xveeds. Farmers cannot use soil-incorporated herbicides in conserv ation tillage systems, in which the ground is left untilled to reduce erosion. Researchers showed that the ability to spray Roundup or Buctril over the respective genetically engineered cot- ton xi eties is a mijoi aidiintige in con- servation tillage. The soil-applied herbi- cides commonly used now are applied betrie plants emerge. With the bioengi- neered cottIon, iarmers can wait and see when and w here weeds develop, thus avoiding unnecessary pesticide use. Als, Roundup and Biuctril have no residual activity in the soil, miaking them environ- mentally sate. Glowing Microbe Aids Disease Study AAES plant pathologists have used genet ically engineered, glow-in-the-dark bactci a and space-age camera e(Iuipment to reveal how disease-resistant plants con- front pathogens. Understanding the mech- anisins of disease resistance is necessary before these defenses can be bioengineered iito susceptible plants. Scientists transtfi med pathogenic bac teiai to express a gene isolated fron glowing microbes, which are associated with marine fish. The luminescent pathogens xere Used to infect normal 1> hb bage plants. Using a Charge Couplcd Device - a high-tech camera used bx astonomers - researchers produced re~il- time pictures of the gloxing pith of the disease as it progressed throughout infectted leaxves. This non-destructive procedure alloxved scientists to spot diseased tissue, even when symptons could not be detect ed by other means. Researchers charted the disease from very early to very late sta''e> , infection and took small leaf samples I analyze the biochemical warfare bein_ waged at each stage. Using these tools, AIiLirn reselili~ Ir showed that iesistant plants produce a Co n- stant antibacterial environment and li prepaired before pathogens invade. Resistant plants benefit from stuady, I xx' level production of seceral defensive bio- chemicals. This production shifts into hib) gear xxhen pathogens enter, illmox\n rciel rant plants to react Cllickly In response to pathogen x allon, resistant plants produce certain peri oxla es, which are enzymes involvet in toughen- ing cell walls to defeni against pathogens. Non-resistant plants may also produce these enzymes but too late to stop the spread of disease. Researchers also foLnd that resistant plants steadily produce another enzyme known as CHL2, xhith is thought to degrade pathogen tell xvalls. AAES ANNUAL REPORT 14 AAES entomologists found that the tarnished plant bug, which is an increasing problem in ~cotton production, is not affected by traditional pest management strategies. However, their ~ iresearch indicates alternative approaches to control the pest. CnSrsonsiblc 101- ths (kIct\ ivc~ eh- tics to Manage Cotton Pests Insectcde s I'CflVLL on Itrl the 1I I i i thetnihdJA~l bug. Belt tlu\w Ih~it t he need ft() bll. wee\Lvil tiigclnt i" it.. c, tarn i~hc J plnt buigs 1M\L \C VSnru mci gret t hlc int AflkL im x vvaN' toi Ater tihe tst's htt it tocut ("III L\\ it eis and arc cxt rcilll'I\' tIUlt.c. W\hen t he XvCc, a c arc ,Ir dirbed Miatrt inigrt ing. (1itc littling that might p reterred ht. t plant ftonr t hc~c inscts I lowc\xvcr, t hcy xxiii Cross t t n ficelds in huge numbecrs, cating ,Is thc g. AAES ANNUAL REPORT I S JnfhII ttlteIy, this iliiss I vIX')eIIt often Occr w l \hen flowei buds are dev'elouing. nl 1st Ither insects, fenmile hugscan holid onto their eggs dlnd deposit them at will. Femal~es wVil layi a few' eggs o n miny 1 plit bet tc i nding the ideal host o n wxh ich h dump the mnijo rity of the eggs. The idea I lhost is 1n(t Cotton, hblt signitic ant ritmber. iii eggs die laid O11 cotton is bugs mfigiate. Since the tarnished plint bugs ire jusi passng through onf theii \\ ay to moie pie- ler red plints, insecticides and biologic al COWlitol treatmients often ire not eflet i'ee. Rescai c hers plii to seek dltela ni e meth- ods for mninglg the pests. ()ne option incliudes thle use of trap plint s to alvoid is a pireferred host foIr tarnished platnt hugs; ilnce they eniei ani iltalti hfeld, thIey genei- aldo nol[ht leiX e. lniit ing altilti arounidta t otton field oi in strips thirotighioi thle field imighi itrip the hugs. Rm tria Cointrol of Wheat Disease liike-ill rot rot of wheat has the potlential to dev'dstate Alihiimi's wheat Sro[p. I. Jirt tiiitely, there are nol resistant wh'leat vai etiles aind no fungicide I tleilnts proihiil COilpte control. /AAES plant lReseatt hers suirveNyed whedt fitelds in AAES ANNUAL REPORT 16 AAES plant pathologists have discovered beneficial bacteria that could control a devastating wheat root disease. maijor wNheat produtctio n areas of the state to ilate root-ass1ciated kic rer in to sree n as potenltial biololgical Iontrol agents for suppression of root rot A total of 1 89 Psendornonos ad Bac ilis species w~ere screened in greenhourse studies. Preliminry stuties showed thlit two (It these bacteria were at least eqilil to BiN tin in root rot co~ntol. Biytin is a tingic ide t hit slippress- es rake-al 1 in the early season but dissipates ifter about six to eight weeks. Biocontol agents colonize the roots and contintie to suppress disease throiughotit the season. In addition to suppressing the disease, one strain' showedc signs oIf increased plant growth, while a second had significantly higher populations on wheat roots. Researchers are now screening the two microbes on wheat planted in November at the E.V. Smith Plant Breeding Unit. Sesame: Cash Crop and Nematode Killer Nematodes can devastate cotton, soy- beans andi peanuts, but most oIf the effec- tive pesticides to control these microscopic worms hav'e been or soon will be banned. In a broad program to develop alternative strategies fot managing solilhorne diseases, AAES platit pithologists hive ident ifie. many plants that can be tised in roririon with cash crops tto Cotrotitl neimatotdes. Hoxwever, thetre ire no mairkets tot mottst of these plants. Research in 1995 showed that scsame is a natural nemattidie that has the poten- tial t bec onie a inijor cash ci op. Sesame is soild~ fot 25 ceflts per potind in the U.S., xwhich mleans that the break-even point for prioducing this croip in Alabama is 400 poutnds pet acrit.. Researchers fotund that exven sesame tteld~s levele. by Hurricane Opal produitetd thit amount tit seetd. In the best sittiations, such as xxere seen in Belle Mina, sesame protduction illowxetd a pntljec- edl $100 per acre profit. These yield~s xxere 30-50 percent below average since the. c top xwas harv'estedl with inadequtate equipment. Sesame reqluires intensc management an. spec ializedl harxvesting equriipment. Reseatrchers are nowx trying to determine the best ways to grow this crop in Alabama. Six sesame cialtivats stippressedl corn mon nemaitodes. Twto cutlt ivar-s cut ptiptli- titans O root-knot nematid~es to zero at the Plant Breeding Unit near Taliassee; tine cultivair yiel.etd more than 500 potinds per acre. At the Tennessee Valley Substation, one ctiltivar ciut poptilatitons Of spiral nema- toides to, ftr per 6.,1 cuthic inches tOf stil, wh ile yielding 851 Pouinds per acre. Another cutlt ixat retdlucedt these nematodes to 30 per 6.1 tcubic inches an. N ieltded 1,135 ptounds per acre. In this test, ct.totn ha. 85 spiral nematodes and] yieltded Il,1 78 ptiund~s he acr e. Microbe Contr ols Disease aIt,i Vt r AAES scientists hive ditcumitent et. fOt the first rime that a beneficial tot-assoi- ite. tnic i oaIiganitsml nor tOnly can mike at plant moire resistant rto a tdisease pit ho gen, but also can redutc e feetding by the insect that transmits the pathogen. Researchlers id.ent ified~ bacteria that are better than weekly pestic ide applic at itons at ctontrt iiling bacterial xwilt tdisease ant. the cuctimber beetle, wvhich spreids the d~isease. Normally, Such bioilogical control agents ate very specific, teit her affetting the d~is- eise ot the vet.tot, but not both. B~acteriil wilt is pitartilirly tdestruc- xie to cucumlbers and~ mtiskmelonS, btit it alsto affec ts squas~ih, zuctct.hint, ptimpk ins, xwatermelons an. cantaloutpes. Insec tic itdes targetctd againist the Cc c mitber beetle Com-ti prise the primatry ctontri Amiethodt~ for bacte- ria 1 xwilt. Hoxwexvet, insetitt.ides art. not very effective becatise beertles ire highly imtobi le an. ntot many beetles are needed to trans- mit the disease. Duing Observations tf f i ell experi- ments to confirm the c'ttecttvenesS of [bacte- reseirc.hers ftouind t hit the tic Krobes wetre also prtotec.ting against hactettial wilt. Stibse. 1 tient gt eniht use and f ield tests Iden- tifted the broad-spec trumi effec t of these bac ieriai aginst hbacterial wilt and cutcutm- her beetles. When appl med is a seed twrar- ment, the bac.teria pipuilate ia plant's rootts xxlitle Plant. Tlie bacteiah boos~tt a plant's iiitine Systemi, muc h like at vaccinaitiotn in aimanls. In iaddit ion, tihe mlicrobes reducit.e the plants that gr eatly Stimutlat es belett feeding. Absenc.e Of th is cot mpoiuitd ireduices feeding indl t rinSmissiin tif hit.teril ilii bet ween plants. Bittchemi.ailly, the Ptrecutrsttrs fttr tctcltitlitat ii attc tiht samer is thie precuritsors for plant defense ne.hinistms. It is ptosstble thit inst ead~ of pitiduc.itig the feeding st im- Ulant , the bacteria t.hinge the plant's ivit- bolt. pathway to Ptttduite dteensive ttttl- Two~ batterial st rains wxere sho wn tot he tmore effet.tive thin instecticties. plat. trieit ed lwth the motst effeti ve staitn wer tnfeSted with iO Peit.ent fex'eit. lit.um-1 ber beetles, exhibited a ftour-ftiltd Jet.relse in bat.terial xiii sinpttinlS and Prttduted~ 35 percent tne Cu lt.umbets. AAES ANNUAL REPORT 1 7 Nev<< 'Niapiw A~'ainst Aflatoxin AAES scienist ~s have identified bac- teria thi-it greatly reduce peanut infestation Xwith Aspecrgillus flatus fungi, Xwhich produltce the c artcinoigen iflitoxifl. Th is t inding, the itlatoxin problt'm in A lihami. Irrigatlioni is thle oinly ettht iX waiy to confsistentlyN reduice ifatix in but is not iliX ys A-fo rdiaimi. AAES irtstarch linkedi lNsCr t irnst ilkV borer teedling to the sprtadi SPOD-X LC, a uew biological iiseciiucid uidei evilUation by AAES researchers, was shown to be effective at controlling beet armywormis. oft A. flat us, but inset ticidies to control lesserl do no [t comupletely cointro i titoi [. (ither studies showeVd that elevated soil cal- citiii reduices atlitlixil. Comtbining insect cointirol and soil tcitility wXith a bacterial occ li Cice of thiis contaminant. Researchers collec ted and stcreened mort than I150 batcterial strains tor activity against A. flat us. Five of the most promnis- ing strinis Xer t~Iest Cd in I1995 at the USDA National Peanut Laboratory. (Xnri otl Itti nviiol metilnt phlots wXeCie used to hipli. atC the extiremel heat intl diriiught that pri imote growith of lesser tcorinstalk borers and A. flatus. TwXo of the bac teia reduced Ataltox in contaminition by morei than 90 percent. On av'rgC, the bac~teria redutced atlatox in tuontcntratioins to approximately 20 parts per billion (pph), which is within the icc CptabC limit. UtinrateJ peanuits hatd 200 ppb it aftatox in. Treated plants also pirodutced a greater yield. 'w Beet Armyworm Insecticide Thinks to research by AAES ento- molIotgist s, t toii tiriers wvill soion leXve a in\w inin-c hemita we X apon against the beeti ii iy'orXXii , Xwhich dievistatedi the c otton cOP in I1995. POID-X LCi, a hio ltigical insetctic ide in the final stages of field test- ing by Auburn restartchers, t iuid become the best way to controli beet armNXworms. l\ )l )X I ( XX, oriiiginaill irketedl fot use on ornament ii plants. HowXXever, AU scijentists recognixted the protiuc t's po tential anti instigatedi stutdies of its use on cotton in 1993. Beet nrms XXorms are major cotton pests uiidei thei rig 1 hit condit 1 ins, intl there is currentlIy nothing on the market to pro- vide adiequtate conrol ot these insects tdur- ing heavN infestations. SI)L) X LC con- rains a litlirallN icc urring viirus that k ills armNXX'(ris Xwithout harming other insects or humans. Once eaten bNy larvae, vital infection iiteralNy tdissoilves the insects from the inside ott The product proividies excel- lent atntmXwi rm co ntrol, even against insects iesistint to chemical insecticitdes. SPOD) LC's pattern of action is ide- ally suited to the development and impact ot thle ii ilN X 11t. In cotton, it is usualNy not the tirst generation ot beet armywXorms that causes majoi damage. A new genera- tion of atmyXX'trms is protducedi each three to toui Xwteks thioughouit the season, ani there aie greater numbers in each genera- tnin. 1.l t imately', a huge popuilathin of wXoms late in the season eats the cotton toliage, blo oms andi bols. SPOD X LC is appliei early in the season Xwhen insect numbers atc low, thus prev ent ing the over- XXhelming poipulation growth. When insects tie they become resitdual applica- tots, with each dlead Xworm spreading more virus to liter generations. AAES ANNUAL REPORT --- 18 FOOD safe t QUALITY & nutrition recent years have seen several major outbreaks ffoodborne sease, creating )ore interest ipfn ever bef'i icontrolling Jimonellh' Id other ithogen% >ntinue to pose threat to UJ S, ,)n sum er iforts to i prove the .ality and rnuti- ve value )f co ,od supply entists in the AES made ajor strides in 995 toward 'complishin" ro~se gcmk Nutrition and food science research to improve infant formulas is one example of the AAES food technology program. Irrprovinp Infaxtnt Fo1ii AASnutritiois~its ad fluid scien- tists~ halve etine ia step clse It io maiking infant fortnilis thit proide all the beneftir' of breast inilk. Researcehers test ed ia promis- ing st urte fton- in essentijal omega-3 tatty it.id thatit frniula lit.k,. )t her soirc.es of this fit ty aid lve ncs"itix e cite. ts on grothii, but ia tatty aid I prtodiced by mic.robes appears to prov ide the nuxeedd benefits xwiithout any drixxhki. cantr amount, oi t he inegi 3 liii t il doosdl-ixaenoit. ait.d (DI IA), but irdi- tional formutla Ihas nne to tis cssteuiil lipid. PI Mk is Monnd in the ret inai iind hbrtin and is believedo to be invoilved in visuail at.uimty and rnt l desC~t pnuent . In prcnmi nurc ihis, eyesight is affec ted by the lit.k 01 1P1 IA. Di1 IA may alsti li\~ i tile in imellI gent. . Adnlt s synt hesiz P I IA ftin intither fatty ait. ltilld lirtileici ai.l I It xx eer, bibies must get PI1 I A ftrtou breast milk tr itItrnuili. ish til is ia good stiurce ti I A hat prcx'ious resent. B shtowed that it inimmhit thle stus tia hion it iiitml relii ed AAES ANNUAL REPORT 19 .r W Controlling the spread of foodborne disease pathogens such as E. col poultry products is a major goal of the AAES. Using piglets as models, researchers found that for mula supplemented with miicrobially produced DHA did not dec reise growth of the animals. Pigs receiv- ing the suppleincnted formula had increased concentr ations of PHA in the brain, retinas and other tissue. Researchers ate now designing experimental techniques to test the functional consequences of the lack of DI IA, with the goal of dlfining the exact role this fatty acid plays in physiolog- ical processes. .Iling Foodborne Disease Ii ee-flo at ing Salmoella bacteria are sill\ killed in poultry processing, but pathogens attached to a chicken's skin are difticult to reach by traditional methods. However, AAES poultry s"cietists have developed tech- niques to remove most of tie firmly attached Slmonell. The topography of chicken skin pro- tects bacrea from the acids used to lean ioultry. Adequate kills of skin-attached bacte- ria require 2-6 per- cent acid concentra- or- Salmonella in tions, which are expensive and discol- or the skin. However, acid solutions of .5-1 percent killed up to 99 percent of Salimonella when combined with the emul- sifier Span-20. Only 20-30 percent were killed \when reduced-acid washes were used alone. Emulsifiers, which hold together oil and water, likely force bacteria into contact with the acid bath. Citric and lactic acids worked best in these treatments, and effec- tiveness was enhanced when the treat- ments were applied in scald baths. Researchers will use the Span-20 treat- ments in a processing plant pilot program in 1996. In other foodborne disease research, researchers found both inherent and envi- ronmental factors that affect the heat resis- tance of E. coli 0157:H7. Some E. coli iso- lates are naturally harder to kill by cooking. Scientists also found that E. coli heat resis- tance is improved when fat, salt and cer- tain spices are added to chicken, turkey, beef and pork products. However, additives used in fat reduction formulations decreased heat resistance. E. coli was most heat resistant in turkey franks, tirkey ham and turkey sausage. However, USDA heat- ing guidelines still seem to be adequate to control E. co/i. Federal law requires that raw and cooked meats be labeled accurately as to the species they contain. Prevention of substitution and adulteration of meats with undeclared species is important for eco- nomic, religious and health reasons. AAES food scientists have developed an inexpen- sive, accurate and rapid test for meat inspection. Tests now used by meat inspectors are expensive, variable in quality, have a poor detection limit, require overnight incuba- tion and cannot detect species in cooked meat. The new Auburn test overcomes these problems. AAES ANNUAL REPORT 20 This new technique uses imnoclonal antilies (MAhs), which reatct with cer- tain proteins in cooked poultry meat. Food scientists dev\eloped six MAhs that can be ised in a series of tests to quickly identity the specific species of pouliry meat used to adulterate products. The tests tan he used to inspect raw products it they are cooked briefly. In addition to merely detecting the presence ot aduilter at ion, these tests can quantify the extent of containination. They also can be used to measure the maximum internal temperature that the meat reached when it was cooked. Resear chers are now developing MAhs to detect mamnnalian muscle proteins. The tests are suitable to be made into commercial kits that can he used easily in the field. To use the test, cooked meat sam- ples are ground and extracted in a saline solution tor 10 minutes. MAhs are coated onto a small dipstick and dipped into a vial containing the meat solution. Results are immediately obvious as the clear solution turns green if the target meat is detected; the more adulteration, the darker the color. Decontaminating Beef Trim with Intense Flame AAES meat scientists have developed a method for using direct flame to deconta- inate beef trim ised in griumnd I products. Searing the surface of beef trim with a high-intensity flame was found to signifi- cantly reduce bacterial populations without harting product qtiality. l)irect flame rt six seconds ctrd popu Lations if batteria that cause 11121 spoilage. Ground beet subjectetd to th, treatment, packaged and stored in a it 111 display case had a shelf lifte iboit twon I lon gcr than untrcatcd mceat. Rcscart Lir are now examining the use of direct-i]n treatments to contrl E. coli, SalmIIlk Im, and Listeril. Traditional methotds to control I),It ria in beet processing Occ mir at the whohe carcass le\'el. But there are many steps between carcass inspection and meat griJ- ing, leaving plenty t Onpportunities for recontamination. The new Aubrhnde\'c I oped technique can be applied immediltcI\ before grinding. In gtround product pIepa a- tion, beet t rimi is transportedc via at coinveyer belt to a grinder. AAES scientists added at new piece of equipment to this process: at chaumber that projects flame on the topl and bottom of tmeat. This chamber is asscmni d around the conveyer just before it tec~I into the grinde. The systei can be idi id to existing pino~c l i'I;ln \\ iilb mit In'-c Iapitil ()il N. AAES researchers have developed an innovative new technique to detect adulterated meat products. r 'it A' -, A ,JL 4~s AALS ANNUAL REFOR! 21 2 ~'CALITV~ our population gows, indI livestockc, cIo il timber productio be(come more intrir Alabama's woteriy tomus, forests ondi other naiural resources ore increasingly threatened. Staned agi~utr I ud Forestry produci,, us well as the health and we//-being of our i izens, depend upon protecting these resori- Safe disposal of animal wastes, low-imp(; timber harvesting, prevetion, of pesticide runoff and other vital environmental promnor goofs have /in hoeen the focus ofAA[ cesfl chi O SLI Ii bLu usu uA AiIu I uS vast u i Ow I usw cus Is a gut dhat mniy AAES cI Us ICry 11 (n agicultur al engines AAES ANNUAL REPORT 22 Reducing Environmental Impact of Timber Harvesting Abundant fairest land iS aman11 thle stiltheast's tnt st valuable natural resaurCes. Sustained use at this resource withoutt dam- ging the en'eironinent is a goal addiressed in cvenil AAES forestry prajects. Developing I ipraved mnanagemn t practices, new~ tech- nology, and a basic understanding at how~ latest systems function wXill allow sustainable ulse af this region's torests while minimni-ing CMi iianmenrt impacts. (one recently Coimpieted Study Showed II ii timber harvesting causes fewX probleins wr the envirunmental health ot wetlands is long as proper imanagemecnt p act ices ai e sed. There is great patential tor harming forsted flood plains, but there are Simnple, ammnonl 1 known w~ays to avoid this damn- ge. If these best mlanagemecnt prac tices are oIlt wed, wXetlands are nut affected even iI I wo- are clear-c~it. I tuiestry teseirchets are now~ exatnin 1i1 lit XXsilviCUlt ural practices affect lang- etim prod~uctivity ol a farest. In this funda- m iental research, scientists hope ta learn iuore abaut howX the tarest ecalagy func- It ins, su1ch aS haw flooding utter ts nuitriet WIailability. Such into~rinationl is important I)CMS erlis tgging raad installatian tit high- wXy i\ kist It tiin Itit5 a tltUtk ptlain Could affec t flootding patterns, whirch, in turni, Cuild attect nutrient av'ailabilit. I nidovert ent sit ts in nutrient ava ilabi lity ian hiarm thoodplain pr aductivity. (fn the l)Ueechee Riv er, researc hers built con- trolled areas to study the effects af flooding on nutrient ava ilabilIity. They partitally buried sections oft luge pipe in whirch river wa ter* is pooled tor vaiouais periods. With these artitic ial icrocosims, reseairchers are Sttidytillg m1ass, Ca rban, nitrt gen anld phaS- Ithil us dyniimics in dertunlpaSing ttiliii lit- ter expo~sed to different flauding iregimnes d.uing a three-year peiod. eit her foirestr tscientists arc ev'altiat- ing mu 1part isf roaid Conist-Utontif, tree hit- vesting and site preparition ol u n imiei ial timber land in South Ahiiua. In tine phase aft ihe study, ireseaircheis ft ind that sail erusian is lessened by the use at grass roads, which aire produced by ;priNying gi ass seed and tertilirer oin dirt roads ithat leadl to timber harvesting sites. (;riXvel iroids were not as etteetiXC hibt did alter better erasion cotrotil thin diit iaads aovei a 15-imanth periad. Atter c lear cutting the area, reseaichers will evilliate v'ariaulS Site prepa- ration tec hn icIuis to iready the land fot replani ing. Pireliminary resullts i lusrated the effec t iveness at "Strainside niani~ge- menit :unes,, strips at undisturbhed land aloiig st reaim banks, in pireventiing signifi cant amounts at sedimlent train) ieaching the waiter. AAES ANNUAL REPORT 23 Reusable Portable Bridges Reduce Stream Damage (Constrlticon and use of stream cross- ings are major causes of sedimentation and erosion in tiimaber hairvesting operations. Sediments can transpo rt other pollutants into waterways, aid in the destruction of aquatic habitats and fill in reservoirs and channels. Preliminary research has demon- strated that AAES-dev eloped poirtable tim- ber bridge systems have little or no impact on streanms if roads are correctly con- - sitric ted. Researchers compared installation, use and removal of portable bridges to o othet co)mmon stream crossing methods in commercial timber operations. After ineasirin g sedimentation upstream and dwnstream, researchers found that the glued-laminated-timber-beam bridges + did not modify stream channels and introduced almost no sediments to the stream. . Cuilverts are placed in a stream ' ' I1 and soil is piled on top, a process that - ?, V c an introduce large amounts of sedi- 4 ~- ment into the water. Also, culverts can -~ A become clogged and cause even more soil to wash downstream. Vehicles dri- -d i ving across fords churn up the stream " a''bed and can introduice sediments. W- However, the only site disturbance Upst car~ and dowinstr ear water quality tests showed that AAES-plo- required with a portable bridge is level- duced portable bridges have little impact on stream health. ing the stream bank for placement of a mud sill, on which the bridge is placed. This disturbance did not affect the stream banks ot the channel. Unlike permanent bridges, the portable structures are easy to install and can be reused many times. If a portable bridge is installed at least 10 times, the total cost tor each installation can be as little as $2,550, which is competi- tive with the cost of installing permanent culverts on most streams. Researchers also developed a special- ized portable bridge that reduces the envi- ronmental impact of skidder traffic. Skidders, large tractors used to drag trees from a harvest site, are nut allowed to drive through streams. And since they are used in a given area only for a few days, con- struction of a permanent crossing is not feasible. Now, a skidder must travel around streams, greatly increasing the machine's route. As the length of travel increases, so does fuel consumption, forest damage and soil compaction or erosion. The AAES portable bridge consists of glued-laminated timber deck panels equipped with steel pro- tective plates, which allow the skidder to pick tp the panels and install them. AAES ANNUAL REPORT 24 5sS (a.tSfQ B rl ) t * Reducing Broiler Litter Pollution The potential tor wvateir qualty dam- age by improperly managed broiler litter is well documented, but many people are unaware that this poultry industry bypr d- uct can Cause air pollution as well. When spread over pastures, litter releases gasses that contribute to acid rain, global warm- ing or ozone depletion. AAES scientists have long studied the water quality effects of litter use and are now developing a method to determine its air pollution porential. Broiler litter teed, wastes, and other materials that collect on chicken house floors - presents a major disposal problem for Alabama's $7 billion poultry industry. One traditional use of litter is spreading it over pastures as a fertilizer. As the litter lies on the ground, ammonia, methane and nitrous oxide can dissolve into the atmosphere and contribute to vari- ous pollution problems. As a first step toward developing management schemes to reduce these emissions, AAES agronomists are refining a new device to monitor ammonia release in the field. This device consists of a 10-foot rotating mast that turns in the wind. Extended from the mast are rods that hold glass vials containing a chemical that traps ammonia gas. The device can measure the gas flow at different heights. To test the monitor, researchers coat an area with urea, allow the device to coIle ct samples rt three day s, then coin- pare its findings to a traditional reterenc e technique for measuring gas emissions. Other AAES research has demon- strated v. alumable uses to hiroiler littei, but it must be used wisely to avoid overloading" ground and surface waters with nitrates and other nutrients. For example, researchers demonstrated that applying eight tons of litter per acre on conventionally tilled cotn/w\inter rye fields in the Tennessee Valley was enough to cause \\ater quiality problems. I lowxever, t uu toins caiused less contamination than the soil test recom- mendat ion foir commiferc ial tert ilIizer. Tbhis lower rare provided corn yields equal to fields treated with c omiierci.ial fertilizer. On the other hand, leadching studies under seedling loblolly pines in the Appalachian Plateau showed that applying iroiler litter to these young trees is unwise. Research showed that pine seedlings lick root systems extensive enough to c apitire the hulk of nitrogen released firon lit icr. It is posbe however, that litter d ouild be safely used at the mid-rotation thinning, about 16 years into the timber production cycle. Research continued in 1995 on the use of artificial wetlands to treat effluent from livestock production facilities. Disposing of Livestock Wastes Finding environmentally friedly w~ay's to dispose of aniimals wvastes is a iuriilo c hal lenge in m he bee, dii, poult ry and swine industries. Agricultura l engineers continumed studies toI imiprovwe the ette ici- ness of constr-uc ted wet lands in trating liq uid waste bef ire it is applimed ovier Iild. AAES ANNUAL REPORT 25 lRtscarchcri lliti ithii it teinpeil- ures above 50 degrees, l ive plants in the t reatment ponds are best at t aptut ing excess~ nutrient,. I loxxever, after the firs hrost, plants at tual ly add nutrients to h water L. From in id-fletember to inid- February wo oden rods driven into the pond bottom proivide better treatment; rods prid e a gron wth med ia tor- batcteria that break down xx aste products. Fngineei- alson determined whic h plats prodrite the greltest biollnass in ton- struilcted wetlandts. Nutrient harvestingv and rmval is dir etly pironporionl ro rhe bio- mass producted. Al son, the harvested plants ran be used is a feed for livestocmk. Ph m tluw~litoeS u1107dlt ( omumon i eed ) prov\ed to be the preferred specijes, prodUt ing ilmn ist twic e as much hbioniass as the next c lonsest plant. As an aii ernative to uising wxet lands in liquid waste mnliilgement, reseamrcheis pre- viuusly designed at novel process with potmentia iri abatinmg polluionn and provmxid- ing at nexw on-farm energy source Engineems dlevelo ped a "suspended pamrtic.le-lttcbed gr owth" ilaerobiL fermlenter, xvhic h tan tuiin liquiefied aiumil wvastes into usable mlet hine gas xxith in four Llays. Th is devic.e iin be uised xx'iih th hyKdirauilic flushing sys- tems t nonlNll used to transport xxlstes outi inmfnimmal priniduttion building s. Based Onl eight y~ear-s of researc h, engi- lCI-mus dlee olud crsi tIiter ii foi oi'lnit Monitor ing and preventing pottution in Alabama's streams and lakes is one of AAES' top pior0ities. loainig roies, hlydriaulic tIdet limit times, and tenpm itures for beef, dii ry, lay er andI Sxx11 me xIStes. Stuidies also iddressetl pr )LLs, dlesign variables suth as reactor metlia till rio, in ndii porosity and media type. Tank; tan be made inf epoxy plastic or fibre glalss and are patcketd wxith a synthetic fibre simi- lilr to air tonditioner filter media. Wast e floxxS into the bottom of the tank and remeimns for- xarious rumes bosed on the xxaste type Methane us piped out of the tank's top ,ad tan be pumupedI into at still age rank oi usedi diretctly in spate heat ti,, internal combustion englines and othei talrm equipment. Other met hods of methane pirmdui on fro m igrictu~ra walstc tiltke 20 days, whereas the newxs tein pr nit sses xxaste iin thlree or touri d l\s. Pollution in Alabama Lakes AAES fisheries reseirc hers havx ceni pletted xxattr quality studties on for majo Alabamm lakes -West Point, Eufaula, We/iss Indl Neeley Henryin and will tinm- plete a stuidy of Lexxis Smith Lake in 1996. ('nntinted uinder the U.S. Enxirninmentil Protec tion Agency 1 's Clean Lakes Program, these studies indicate problems with toxic contaminiiniiii iin snomt lilts iand high Icv- AAES ANNUAL REPORT 26 ~. '. - vJ \ ic contaminants were fouand in the it tililtochlie River reservoirs Aest Pouint liid Euifatim- and tn thme txx't 'oo5i Rivei reserirus Weiss aind H einriy. 1iloi dine, in insec tic ide that wals onc e I 1512( to conltrol termites, wvas the ifl11st sig- niif icant problem in the Chattilhoochee I eSem xomrS, wxhie industrial PCBS were Ilund in excessive concentrations in the ( Coosa reservois. PIy examininlg tish inl ,(tfnle lakes and sediments in all, researchers determined the distibihmon of toxic o(n!a- ininants. For example, c hlordane contai- I it ton xxas iiited tt I the very uippemo st Ii tion of Lake Eufaula. Public health otti- Is have isstied adv'isories to pro h ibit coIn- inuption of fish from areas oIt these reser- dirs. Int erest ingly, the health adxvisory for WVest Point Lake is based on high con en- I rations of tintam inalnts in catfish a~nd Ii lgeifltltth lhiss, but iAuhtmrn studies folund iI(I Sigiicanit conltinaiitioni in black crap- pie and hybhiid striped hiss. In ai related , tidy of the IHuintsville Spring I ranc h/Indian Creek tributary to the WVheeler Reservoir, researchers totund that I )l)T disc harged from lit mantiamttring a~knt alimolst ? scari ago still pcr Iis. Chlattahoo ichee and (Coo la rivxer reser- irs also have significaunt prt Ileinl wxith iltr ient enric hment, or elevated attn hits n iitrolgen aind ph ii Ihrotis. Nutrint cnri hment causes exc essive girlvih 4f plankt on algae, wxh ich can . itme witer q1uality\ probleims and fish kill,. Sewerc ox ri tlxxw, diS. Ilrge of treited was! ewit er liid rinoff fronm i~i. ulttiral and urbhan iareis aic iflijti CiMiSCS of this ntltrienit l\ crld. West Point Lake, in particutlir, s'uffers fro m the Iimpac t of nutrients' filowing ftrom the At lintit area. Studies Showe d that at Gcorgia bin o n high-phospllt e deterigent, ani l Iimits placed on phosplhorous ini treat- ed xvistexxater have dec.reised pho splhorous Iladin~g ini We(st Poiint iand ILike iifiiila. Hlowever, further ireduw tions coul b11 e need- ed to offset the effects of plinlied inc reases in treated wa stexwater dischl; ges d ant ic- pitcd reduc ed tribtary flows." iaised b\ incrieased wxater cnstinilpt n upstreaml. Sntith I ike has sonic tribuitarics thait aie impacted Iby (Aontaiivitiltrfilii polilt rI cit Ie and tither agicu tltutral l1)crii l. AA[ S researchers are wvork ing to deter- mine tihe efticts of inutrienit enichmitent oni receiving wx ters; 1 996 dat a wxili he c im- pared to restults frtmilii simiilar st udy con- duc ted in I1986 to determine wit er quill it y chiainges. Estinmtes> of nut rient and sedi ment Iladiiig will 11e made iisiig satellite AAES ANNUAL REPORT 27 Tools to Stop Herbicide Runoff AAS cent is foutnd that under th hest Uf LiirL umlstaIces int nirsery proidui- tio n, 25 pec n of~l (th[I herbic ide applied t, plints mnisses target p itS and is xxasihed ixx ix. How\ever, resell cher s lixve dexvikl~~ txwo tecLhniquies to greatly redCJIL th is p itenftial sourL of waxvter contamnination.i Baised nlmi earCit r sI 1tudy that shu xi heriLIci aC tighly N bound in theC uipi Livr it pi ltting mted ic, researIchers dCvc I iap'd a meth od torit Using med ia ti filt Ci tiidcs out of runoff water. In lab InuJels, meldIia \Xs placed int plistic plies, eCl IIiM Uil ch end il wix re mesh. Thirty per- ceCnt it heriid re IC siduC wa s fiti re fUrCtmli water thai fli wed thiriugh the media filtir Longer expo suir t imest removx d uip Ito 70 peClt Using meldia, a product availale iin largC qiuaniie cot iitrseries, is much~l simnpler ilkd c heaipr than the activated Lhrca tli iiiIhat S111k iirtir ies use. ReCsar c hers LiionliItCtd a series of lab- iii iior stieIs an~d arC nowX\ cinduc~tinig theC tirst fie~ld studies wi th the media tilteri. WO(ilh ilhe i'(ial of refining theC systemi ft-l a vairiety iif titctirs, inlulinlg iiedia pariti- C sieC ad t he use it diftereiit miidiai 1111- uthiily totijid in a nurilsery. I IlriLic IIcS i trdi~tionailly~ briadlcast appliCd, mneaning thtat they Sprayed ovxer Ihk hip ot ai groiup Ai tr-get puts. After deterCining that heriL des wh iLh aCtually AAES scieiiusts developed a prototype system foi filtering excess herbicides in drainage water from nursery plant production. h it the INtS dii not leach fromi meldiai, .\AAES researchers deveLloiped a more pre- Lise method for applyiing the che~micals. Ciombining the herbicide xvith thLe fertilizer, xwhiLch is applied diirLLtly into the pot, rL'mLdies the problem of herbiLcide loss and does not requiire extra labor. In the most ettectixc Cfturmula, liqluid herbicide is mlixed wxith xxauter and sprayed on fertilizer in an nduistriaii bMlndler. TheL optimni u cated firmulatitin is eight poundls ot Roinstar 5O'uNP hericLide Lcoated onito 25 pounds of Nuirsery~ Special 12-6-6 tertilizer. Herbicide- Lulated tert ilizer proivided weed conltrol sim-i ilar to broadcast applicatioins, while reduc- ing the amount ot herbic ide runoff to prac- tically zero. AAES ANNUAL REPORT 28 ECONOMIC, AND health StSUES state citizens is one of the fot: ing principles of the AAES. Iss, related to economic develoni physical health and emotic being are the focus of mai Experiment Station investi AlabarniaTextiles on tin, In((, With support from the AAES amid National Textile Center, consumer affairs researchers have developed an Internet database that gives textile arid apparel mnanuifactu rers greater marketing opportu- nities throughout the nation. Through the electronic directory, major retailers anid large manufacturers can contact even tile smallest, mohst rural Alabama plant to .It .. raato., AAES consumer affaitrs r eseai chers hiclped put Alabamna's textile industry on the Information Superhighway. a . oirder pi idutts or otler Stihctl- trac ts. Coi'mputer-assisted souirc- ing of apparel produt ion has hus far generated it least $50 il lionr in A labama sales. Tis1 Naiti tinl Sourcintg hlbase me ets th1-e need ti i a single, tentrali~ed source of mint 11.11itn o th le nationts c urrent ly available text ile md ippml iproduct ion capac~ity. It uilta~jin in-depth infuirmaition on eac.hI plants ca pihilitites, including such tat iors is in i mumll proidiuct ion limttit, types oii t asks. Buy ers searc.h the data- kase by producit tcategorly to find it tues that meet thteir specifi- Iniortttition on Alihinii iail fiturI Pteias first t t in- 11iled into a proitt type dat ihise in 1994. Fac t ti es in G eoirgia amd the ( arolinas were subse- (Iuently added to tthe syst em. AAES ANNUAL REPORT 29 rr+-' a-~':' -' .9 AAES resour ce economists are developing plans to better market and preserve Alabama's national forests. Eventually, the database will include all the impact o nation's textile and apparel plants. group of Researchers also developed a simple, inex- urban u. pensive modem package that allows small TNF an( plint managers to access the database and trip. Urb update the information on their respective hiking, l firms. The daiabase is now completely viewing operational and is being introduced to users coL produc t developers in major retail compa- expandir nies, such as Wal-Mart and ) C. Penney's. ties. Lo( Lack of plinnint g for multiple tses and erred ht lack of knowledge about users of Alabama's placed p national foIrests can cause resource degrada- ulation. Lion. AAES economists are developing marketing plans foi these resources and are ,analyzing the recreation areas to identify ways to bet- ter preserve them. Researchers profiled c t ommunity leaders, special i interest grotps and users of Talladega National Forest (TNF) to design more effec- tive marketing strategies. -' Surveys showed that the tser segment with the great- est potential economic i area communities is the smallest visitors. This grotp, designated as ,ers, travels the farthest to reach I spends more time and money per an users are attracted to TNF for Backpacking, camping, fall color and related activities. More urban ild be attracted by improving or ig areas dedicated to these activi- cal and regional users of TNF pre- inting and fishing, which have resstires on the park s wildlife pop- These groups expressed strong pref- erences f)r expanded facility development, which may be costly to provide. However, they were more willing to pay additional user fees to cover such Costs. Researchers identified a need to zone TNF to avoid conflicting uses. For exam- ple, hiking trails now run through areas designated for hunting. A non-hunting cor- ridor in a portion of the forest would ensure safety while reducing pressure on wildlife. TNF's Cheaha Wilderness and Bankhead National Forest's Sipsey Wilderness and Sipsey Fork Wild and Scenic River were found to he suffering from symptoms of site overuse that threat- en to render some sites unacceptable. To prevent resotrce abuse or area closures, researchers formulated multiple-use man- agement plans with suggestions for renova- tions ot new facilities. AAES family and child development researchers are working to identify the iajor stressful events faced by Alabama's rural and urban families and the role of social support networks in maintaining family well-being. Data were collected from 318 parents of college-aged children on life events that had impacted families during the previous year. In general, the sample was whitc, ciu- cared, middle-aged, and abott evenly divid- ed across rural, suburban and urban areas. Restlts suggest that even families many AAES ANNUAL REPORT 30 ~ r3 ' c ~iiin~ r 1L L ~~a i r* s rr- I would view as cushioned from adversity must be able to adapt to and cope with a high frequency of stressful events. During the past year, the average tam- ily reported experiencing four life events that aftected its well-being. The most fre- quently reported stressful events were asso- ciated with financial and employment issues. The most difficult issues for families included death, life cycle transitions and health problems. In coping with problems, these families first turned to social network members. Extended family members wvere mentioned most often, followed by fricnds and nuclear family members. Spiritual resources were also cited frequently as ;i source of solace and support. This study highlights the need for family life education programs to stress sk ill development in providing emotional sup- port to others and in creating and nurtr- ing social support networks. Further, agcn- cies that provide support to families shou ld seek to enhance and work cooperatively with families natural support systems. AAES entomologists have developed a formula that takes into account a wide range of home and landscape characteris- tics to predict the size of cockroach popula- tions and how best to control the insects. This new pest management strategy elimi- nates cockroaches faster, better and loInet.rc, while using a fraction of the pestic ides requiircd inl stanirid dtreatments House age, unscrcened windi 155 S, ws all cracks, loose-fitting doors, wood piles, aim unt if mulch and shruibbery , numbers of outbuildings, hardwx ood trees and pets, and mnany other variables aire factored into the formula. By identifying problematic aieas, researchers were able to control cockroaches with small, strategic applica tions of bait, gel and spray. In addition to l iited inscctic idc List, t he intcgratedl pest iminagement (IP) strategy also consists ot san itat iton and lindsc ape ilnrageirient, such as pruning, removing ivy or using ilternat is e imulc.hes. This strategy is designed tor Smllokbrown cockroach es, whic h ire lirige, Jark-brown insec ts. Entomologists arc devising similar sti It egCs tot other household pests. Trid it it a pcimeter i nsec ti cide treatments applied accoird ing to lIbe directions are etfective for abut 30 days, while the 1PM system listed 60 day-s. Coct.kroac.h abun- dance was tinaftec ted outside the spiay z-one in the pertimctem it at- merit, and cockroaches quic.kly rein- ided from peripherali habitats. 1PM I catrurts sgiiticaritly iece~t d cokroich poipulationrs and~ sup- crssed the insects tor a lonrger pei- / ,I. Plus, 1PMI treat merits ieqiuire 80 pec Ic l~ c"11(1itt h i i". Contr olling cockroaches and other household pests is a major goal of the AAES ur ban entomology program. AAES ANNUAL RFPoRT 31 1995 DIRECTOR' S RESEARCH #1'V IV 10 i u Winners of the 1995 AAES Director' R~esearc h Awar ds were Claude Boy d, a pro- tessor in the Department of Fisheries and Allied AdiiictiltueS; an1d C iteMll ins, an ass)citate professori in the I epairtmnent of Agironomy and Soils. These iwairds ire pre- sented annually to recognize outstanding career accomplishments in the Experiment Staltion. Boyd, a native of Mississippi who joined the AU. faiculty in 1971, has made lie envir onimentAl risk posed by effluents reseairch in the use of Iqimatic plants to fil ter wastewater. Ile also demonstr ated the relationship between teed input and watei dual ity\ variables in ponds, which has helped tish and sh ri mp pmroducers avoid many problems. In other studies, Boyd designed a watei ciiculator for catfish ponds, devel iped techn idtes to measure pond l im~e eqdim mnts, anailyzed the use of fluid fertilizeis in ponds, dev ised a system to more accurately estimate pond water budgets and exaluated pir)duc ts used in commercial and sportfishing ponds. (i'irrent ly, Boyd is evaluating products to imnprovxe pond wxatem duiality and developing relitable management procedur es to reimovIe phosphate fronm pond wvater. M~ullins, a Virginia native wxho caime to Auburn in 1985, has addressed many issues related to soil fertility and chemistry. Foru example, he cx aluated the dry matter production and nutim ent uptake of cotton varieties, generating intornmtion crucial in answxering cotton feirtility duestions. Mumllins also showved that late-season sur- face applic itions of potassium to cot ton is is effective as deep placement, thus saving firiners unnecessary expenses. Anothei of M/ull ins' studies is opening new mar- kets to U.S.-produced fertilizers. Previously, the European Economic Commuinity penalized U.S. fertiliz- rsfor being lowv in wxater-soluble posphorous, but he provxed that 1, U.S. fertilizers are as effective as ' 4 European products. M~ullins also studies alternative crops such as lupin and the use of industrial and Igricutltuiral wxastes as so il amend- 11ents. Left, Senior Research Award winner Dr. Claude Boyd. Right, junior Research Award winner Dr. Greg Mullins. AAES ANN J"I I H 32 u j State $19,611,042 19 Federal I $4,358,858 State-Paid Employee Benefits I $2,040,000 CONTRACTS AND GRANTS Federal $5,002,933 State $1,004,194 Private $4,088,759 AUXILIARY REVENUES Indirect Cost Recoveries $1,099,138 Interest and Royalties $73,568 Diagnostic Services $251,412 Sale of Research Products $3,063,184 r"p TOT A L R E V E N U E S $40,593,088 AAES ANNUAL REPORT 33 0 I t4C 4. *w . f! ~* .4 s eA i wtk f + f Y4 f AbLeA 10 in