2010 National Cotton Fusarium Wilt Report November 2010 Agronomy And SoilS depArtment SerieS no. 310 AlAbAmA AgriculturAl experiment StAtion WilliAm bAtchelor, director Auburn univerSity Auburn, Al 36849 This reporT is a joinT conTribuTion beTween usDa-ars, crop science research LaboraTory, Mississippi sTaTe universiTy, Mississippi, anD The aLabaMa agricuLTuraL experiMenT sTaTion, auburn universiTy, aLabaMa Information contained herein is available to all persons regardless of race, color, sex, or national origin 2009 NATIONAL COTTON FUSARIUM WILT REPORT 3 National Cotton Fusarium Wilt Report ......................................................................... 4 National Fusarium Wilt Trial Figure 1: Field plot layout ....................................................................................................... 6 Table 1: Least squares estimates of the average percent wilted plants ................................... 7 Table 2: Least squares means of root knot J2 infective stage number ................................ 13 Table 3: Least squares means of root knot nematode egg number......................................... 19 Table 4: Least squares means of total root knot nematode number ...................................... 25 Table of Contents Commercial Cotton Wilt Trial Table 5: Least squares estimates of the average percent wilted plants .................................. 31 Table 6: Least squares estimate of root knot J2 infective stage number .............................. 32 Table 7: Least squares estimate of root knot number ............................................................. 33 Table 8: Least squares estimate of root knot egg number ..................................................... 34 Table 9: Least squares estimates of seed cotton yield ............................................................ 35 2010 NATIONAL COTTON FUSARIUM WILT REPORT Kathryn M. Glass1, William S. Gazaway2, Katheryn Lawrence4, and Edzard van Santen3, Advisor III, Natl Res. Programs and Professor, respectively, Dept. of Agronomy and Soils, Auburn University, AL 36849 2,4 Professor Emeritus and Extension Pathologist/Nematologist/Associate Professor, Dept. of Entomology and Plant Pathology, Auburn University, 36849 1,3 Cotton cultivars and elite breeding lines submitted by 14 cooperators were evaluated for Fusarium wilt resistance under field conditions at the E. V. Smith Research Center, Plant Breeding Unit, Tallassee, Alabama. These entries were grown on an Independence loamy fine sand highly infested with the Fusarium wilt fungus (Fusarium oxysporum) Schlect. f. vasinfectum [Atk.] (Snyd. & Hans.) and southern root-knot nematodes (Meloidogyne incognita.). In 2008, a soil analysis for nematodes revealed that southern root-knot (Meloidogyne incognita) was the predominant nematode species in the test plots. The North Fusarium wilt field plot contains a population of M. incognita that ranges from 155 to 1546 J2 per 150 cc of soil with an mean of 711 J2. The populations in the South Fusarium wilt field are lower with a range from 77 to 1004 J2 per 150 cc of soil and a mean population of 378. Other nematode genera present are stubby root (Trichodorus sp.) and stunt (Tylenchorhynchus sp.). Root-knot nematodes, however, appear to be causing the major damage to cotton in the Fusarium Wilt Test as indicated by the high galling indices found on the roots of all cotton lines. The root-knot nematode population throughout the entire test area, i.e., even the areas with the lowest root-knot nematode populations, is more than sufficient to cause a high incidence of Fusarium wilt. Cotton lines submitted to the Fusarium Wilt Trial were examined to determine their response to both pathogens the root-knot nematode (Meloidogyne incognita race 3) and Fusarium oxysporium f. sp vasinfectum . The field has a long history of root-knot nematode infestation. Plots consisted of 1 row, 20 ft long, with 36 in row spacing and were planted in a randomized complete block design with four replications. All plots were maintained throughout the season using standard herbicide, insecticide, and fertility production practices as recommended by the Alabama Cooperative Extension System. Three plants per plot were removed on July 22. The fresh root weights were recorded and nematodes were extracted from the root system by shaking in 0.6% NaOCl counted under the inverted microscope. Fusarium was aseptically isolated on acidified potato dextrose media from systematic plants removed July. Data was statistically analyzed using Generalized Linear Mixed Models procedures as implemented in SAS® PROC GLIMMIX with a negative binomial distribution function for count variables. Percentage data converted to decimal fractions were treated as a pseudo-binomial, whereas seed cotton yield data in the commercial test were analyzed using a normal distribution function. Dunnett’s P-values were calculated to compare entries to check cultivars. Monthly average maximum temperatures from June to October were 90.1, 86.7, 87.1, 81.2, and 70.1 °F; average minimum temperatures of 66.7, 66.4, 66.9, 64.6 and 50.2 °F. Total rainfall amounts from June to October were 1.1, 5.5, 4.2, 4.6, and 6.5 in. The total rainfall for the growing season was 21.9 in. 2010 NATIONAL COTTON FUSARIUM WILT REPORT 5 The 2010 season, environmentally, was conducive for the root-knot nematode and Fusarium wilt pathogens. The numbers of root-knot nematodes increase in all the cotton samples submitted. The standard susceptible cotton, Rowden, averaged 1150 root-knot J2 and eggs per gram of root while the M-315 resistant cotton supported 460 root-knot J2 and eggs per gram of root. Lonren 1 which was released as a reniform nematode resistant genotype, supported root-knot nematode numbers in between the susceptible Rowden and resistant M-315. Nematode juveniles and eggs extracted from the root systems for all the submission ranged from a high of 12,483 in PHY-MM1 to a low 242 in DJ-5. The reproductive potential observed varied widely from highly susceptible (PHYMM1, PHY-FB2, PHY-FB7, FB-5, CW-1, FB-2, PHY-MM2) to low susceptibilities (DF-5, DJ-2, PHY-MM3, CW-5) depending on the cotton submission. Re-isolation of the Fusarium wilt fungus Fusarium oxysporum f. sp. vasinfectum was conducted to confirm the presence of the disease pathogen. The fungal pathogen was not found in the resistant M -315 cotton but was readily isolated from Rowden and Lonren 1. Over all the cotton submissions planted in 2010, 76.25% were colonized by F. oxysporum f. sp. vasinfectum. Entries were planted in single 20-foot rows on 36-inch centers, separated by 6-foot alleys. Four replications of the test entries and checks were evaluated in a randomized complete block design with a split plot restriction on randomization. The set of eight test cultivars submitted by a cooperator was always evaluated as a group together with two control plots within each replicate. Both susceptible (Rowden) and resistant (M-315) cultivars were included as check subplots in the two center rows of each main plot (Fig. 1). A industry-sponsored cotton test was planted adjacent to the National Fusarium Wilt Trial (NFWT) to compare 10 commercially available cotton cultivars to Rowden and M-315 for wilt response, rootknot nematode reaction, and yield. Trial maintenance was similiar to the NFWT. Results are presented in Tables 5-9, starting on page 31 of this report. Lack of mositure delayed planting on May 14. Initial plant counts were made on June 9. Wilted plants were counted and removed on June 23, July 7, July 21, August 4, and August 19. The remaining live plants were counted and recorded on August 26. Total percent wilted plants were then determined and mean wilting for a given entry calculated. The average % wilted plants for the susceptible check Rowden was 21%, with a range from 11 to 42% on an individual plot basis (Fig. 1). Wilt development was low but quite uniform in all blocks with rep averages ranging from 5 to 10%. The resistant check M-315 had an average of 2% wilted plants, with a range of 0 to 4%. Critical evaluations of breeding lines should be made relative to the Rowden check listed at the bottom of each group. 6 ALABAMA AGRICULTURAL EXPERIMENT STATION Fig. 1. Field plot layout and % wilt for control plot of Rowden (susceptible) and M-315 (resistant). Distances (ft) from the SE corner of the trial are given in the left hand column and the bottom row. EW 390 46 2 12 1 0 27 4 21 Rowden M-315 21 364 2 Rowden M-315 17 0 M-315 Rowden 1 35 M-315 Rowden 3 3 Rowden M-315 0 24 Rowden M-315 0 16 M-315 Rowden 0 33 M-315 Rowden 18 0 338 M-315 Rowden 4 22 M-315 Rowden 0 10 M-315 Rowden 15 5 Rowden M-315 26 0 312 M-315 Rowden 2 26 M-315 Rowden 3 24 M-315 Rowden 2 16 Rowden M-315 26 2 286 M-315 Rowden 0 11 M-315 Rowden 17 0 M-315 Rowden 11 2 Rowden M-315 0 5 260 M-315 Rowden 6 0 Rowden M-315 1 6 Rowden M-315 2 11 M-315 Rowden 0 0 234 Rowden M-315 0 8 rows 0 8 rows M-315 Rowden 0 12 8 rows M-315 Rowden 26 4 8 rows M-315 Rowden 17 4 8 rows 208 Rowden M-315 0 0 M-315 Rowden 9 19 Rowden M-315 3 0 Rowden M-315 38 11 182 M-315 Rowden 0 8 M-315 Rowden 0 26 Rowden M-315 0 35 Rowden M-315 3 3 156 M-315 Rowden 14 6 M-315 Rowden 0 22 M-315 Rowden 40 0 M-315 Rowden 0 31 130 Rowden M-315 14 0 M-315 Rowden 20 0 Rowden M-315 27 0 M-315 Rowden 17 5 104 Rowden M-315 0 2 Rowden M-315 25 0 Rowden M-315 2 17 Rowden M-315 3 25 78 M-315 Rowden 20 0 Rowden M-315 0 37 M-315 Rowden 41 2 M-315 Rowden 17 0 52 Rowden M-315 1 15 M-315 Rowden 44 6 Rowden M-315 56 0 Rowden M-315 0 25 26 M-315 Rowden 9 0 Rowden M-315 22 1 Rowden M-315 0 65 M-315 Rowden 2 50 0 SN Rowden M-315 24 Rowden M-315 54 M-315 Rowden 84 M-315 Rowden 114 2010 NATIONAL COTTON FUSARIUM WILT REPORT 7 Table 1. Percent wilted plants for entries and check in each replicate, least squares estimate of the average, P-value based on Dunnett’s versus the resistant check M-315, and initial average number of plants per plot. Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. no. Avg.P-value of plants 46 58 54 62 54 45 59 49 54 64 47 48 36 30 40 45 36 47 53 66 69 63 77 55 66 43 54 39 46 54 O. Lloyd May, Delta and Pine Land Co., 381 William Gibbs Rd, Tifton, GA 31794 101 LM-1 24 13 11 0 11 0.003 102 LM-2 0 8 1 6 3 0.276 103 LM-3 13 6 6 0 6 0.049 104 LM-4 5 11 3 4 5 0.077 105 LM-5 15 6 9 5 8 0.014 106 LM-6 15 2 5 3 6 0.056 107 LM-7 2 4 0 0 1 0.718 108 LM-8 9 4 3 3 4 0.149 Rowden 44 3 17 16 22 < 0.001 M-315 6 3 0 0 2 Dawn Fraser, Monsanto Company, 741 Coker Farm Road, Hartsville, SC 29550 201 DF-1 2 7 0 1 2 0.695 202 DF-2 3 4 2 2 3 0.666 203 DF-3 1 1 1 2 1 0.842 204 DF-4 8 1 5 7 4 0.374 205 DF-5 7 1 1 4 4 0.403 206 DF-6 2 10 1 1 3 0.578 207 DF-7 1 5 1 1 2 0.758 208 DF-8 2 4 2 15 6 0.181 Rowden 25 19 24 3 19 < 0.001 M-315 0 9 3 3 4 Darren Jones, Monsanto Company, 4600 Old Leyland Road, Leland, MS 38756 301 DJ-1 9 1 2 0 3 0.028 302 DJ-2 6 3 0 0 2 0.050 303 DJ-3 0 1 0 1 1 0.295 304 DJ-4 3 0 0 0 1 0.294 305 DJ-5 14 4 2 3 5 0.007 306 DJ-6 6 3 2 4 3 0.026 307 DJ-7 14 5 0 2 5 0.007 308 DJ-8 4 1 8 3 3 0.022 Rowden 20 20 6 17 15 < 0.001 M-315 0 0 1 0 0 † The number listed in the average column is the estimate of the average wilt percentage based on a generalized linear mixed model with the binomial distribution for fixed effects. This estimate will generally be close, but may or may not be identical to the arithmetic average obtained by averaging the numbers in the columns representing the 4 reps. 8 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 1. continued Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. no. Avg.P-value of plants 57 65 51 63 64 60 59 63 39 48 68 70 62 68 47 59 51 52 44 53 Dave Albers, Monsanto Company, 800 N Lindbergh Blvd., St. Louis, MO 63167 401 DA-1 2 3 4 0 2 0.556 402 DA-2 3 2 1 5 2 0.494 403 DA-3 9 10 1 2 5 0.156 404 DA-4 4 5 2 7 4 0.210 405 DA-5 9 3 1 4 4 0.242 406 DA-6 16 2 2 5 6 0.085 407 DA-7 10 1 4 0 3 0.291 408 DA-8 4 2 2 4 3 0.413 Rowden 65 38 5 46 42 < 0.001 M-315 0 11 1 2 2 Richard Sheetz, Monsanto Company, 1596 IH 27-87, Hale Center, TX 79041 501 RS-1 5 11 0 1 4 0.158 502 RS-2 21 21 1 8 13 0.006 503 RS-3 3 9 4 15 8 0.031 504 RS-4 1 8 4 1 4 0.196 505 RS-5 1 10 7 0 4 0.146 506 RS-6 0 0 4 0 1 0.629 507 RS-7 6 16 1 0 6 0.076 508 RS-8 2 0 3 3 2 0.433 Rowden 17 31 26 24 24 < 0.001 M-315 2 1 4 0 2 Daryl Bowman, North Carolina State University, 3709 Hillsborough Street, Raleigh, NC 27607 601 NC1 82 2 1 7 21 < 0.001 46 602 NC2 2 1 1 2 1 0.448 57 603 NC3 13 1 1 0 2 0.171 51 604 NC4 17 2 2 2 3 0.068 55 Kathryn Glass, Auburn University, 201 Funchess Hall, Auburn, AL 36849-5415 605 PHY 440W 5 1 1 0 1 0.469 David Weaver, Auburn University, 201 Funchess Hall, Auburn, AL 36849-5415 606 AU 6001 17 3 1 1 3 0.071 607 AU 3111 22 1 1 1 3 0.053 608 AU 3202 14 4 1 10 4 0.039 Rowden 50 8 16 18 17 < 0.001 M-315 2 1 2 1 1 46 43 42 38 43 42 2010 NATIONAL COTTON FUSARIUM WILT REPORT 9 Table 1. continued Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. no. Avg.P-value of plants Mustafa McPherson, PhytoGen Seed Co., LLC, 118 Kennedy Flat Road, Leland, MS 38756 701 PHY- MM01 11 8 4 3 6 0.097 9 702 PHY- MM02 3 2 4 2 2 0.253 28 703 PHY- MM03 ‡ ‡ ‡ ‡ 704 PHY- MM04 18 2 2 0 5 0.054 57 705 PHY- MM05 1 4 1 3 2 0.305 28 706 PHY- MM06 16 1 2 1 5 0.094 23 707 PHY- MM07 ‡ ‡ ‡ ‡ 708 PHY- MM08 34 3 6 3 9 0.013 57 Rowden 25 26 26 35 29 < 0.001 43 M-315 1 0 2 1 1 55 Fred Bourland, University of Arkansas, P.O. Box 48, Keiser, AR 72351 801 FB-1 14 0 13 7 802 FB-2 14 1 2 4 803 FB-3 3 0 4 0 804 FB-4 3 1 0 3 805 FB-5 7 2 3 4 806 FB-6 5 5 2 2 807 FB-7 12 8 7 2 808 FB-8 11 8 7 8 Rowden 15 22 26 27 M-315 1 0 2 0 8 0.002 6 0.010 2 0.220 2 0.242 4 0.033 4 0.050 7 0.004 8 0.002 23 < 0.001 1 55 56 58 45 61 49 54 50 54 61 48 65 55 49 60 63 67 73 48 56 Brent Styles, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 27893 901 CA-1 10 1 0 7 5 0.089 902 CA-2 0 0 0 0 0 0.788 903 CA-3 3 1 1 2 1 0.472 904 CA-4 3 4 1 2 2 0.280 905 CA-5 6 5 0 9 5 0.085 906 FS-1 0 0 4 18 6 0.054 907 FS-2 13 18 0 3 8 0.020 908 FS-3 1 12 7 12 8 0.024 Rowden 22 40 1 22 23 < 0.001 M-315 1 0 1 4 1 ‡ These entries could not be evauated because < 10 seedlings emerged per plot. 10 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 1. continued Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. no. Avg.P-value of plants 74 57 67 79 76 75 63 81 50 55 67 59 78 66 51 71 66 43 51 65 50 60 59 58 73 68 43 77 49 48 Brent Styles, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 27893 1001 TP-1 5 15 4 3 6 0.028 1002 TP-2 12 5 0 5 5 0.048 1003 TP-3 6 10 0 3 5 0.057 1004 TP-4 29 11 3 5 11 0.003 1005 TP-5 2 7 0 3 3 0.179 1006 TP-6 11 11 3 1 6 0.026 1007 TP-7 4 14 9 6 7 0.017 1008 TP-8 14 8 0 6 6 0.021 Rowden 25 27 11 12 17 < 0.001 M-315 3 0 2 1 1 Brent Styles, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 27893 1101 TS-1 7 6 13 5 7 1102 TS-2 0 0 0 15 2 1103 TS-3 0 4 2 3 2 1104 TS-4 0 3 11 3 4 1105 FS-4 1 2 1 13 4 1106 FS-5 1 1 3 5 2 1107 MS-9 2 1 3 3 2 1108 AU 6202 1 1 3 0 1 Rowden 2 35 6 5 13 M-315 0 0 1 15 4 Brent Styles, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 27893 1201 MS-1 17 6 13 1 9 1202 MS-2 4 6 4 2 3 1203 MS-3 7 2 13 4 6 1204 MS-4 4 4 0 0 2 1205 MS-5 32 8 7 0 11 1206 MS-6 3 0 2 1 1 1207 MS-7 4 7 9 0 4 1208 MS-8 4 0 0 3 2 Rowden 17 3 17 10 11 M-315 1 0 4 0 1 0.189 0.739 0.727 0.476 0.544 0.700 0.724 0.811 0.047 0.014 0.110 0.036 0.253 0.007 0.336 0.087 0.237 0.008 2010 NATIONAL COTTON FUSARIUM WILT REPORT 11 Table 1. continued Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. no. Avg.P-value of plants 2 0.179 3 0.099 4 0.036 4 0.041 8 0.005 6 0.011 3 0.077 4 0.034 29 < 0.001 1 5 1 2 1 4 7 1 5 13 1 0.043 0.413 0.274 0.370 0.066 0.028 0.390 0.053 0.005 54 43 61 60 57 58 48 59 48 59 57 65 70 52 74 46 61 42 51 55 69 72 62 55 61 59 67 56 44 49 Charlie Cook, All-Tex Seed, 356 Hosek Road, Victoria, TX 77905 1301 CC-01 3 2 4 2 1302 CC-02 10 1 2 1 1303 CC-03 16 3 0 1 1304 CC-04 11 5 2 1 1305 CC-05 14 8 8 9 1306 CC-06 17 7 2 3 1307 CC-07 6 2 7 1 1308 CC-08 16 0 2 3 Rowden 56 14 12 33 M-315 0 6 0 0 Cody Poage, All-Tex Seed, P.O. Box 1057, Levelland, TX 79336 1401 CP-01 4 14 1 2 1402 CP-02 0 4 0 0 1403 CP-03 0 3 0 3 1404 CP-04 0 1 2 2 1405 CP-05 13 2 0 0 1406 CP-06 12 9 5 1 1407 CP-07 0 4 0 0 1408 CP-08 1 9 1 8 Rowden 9 14 11 21 M-315 0 0 1 2 Tim Dabbert, Monsanto Company, 38768 W. Farrell Road, Maricopa, AZ 85138 1501 TD-1 5 6 0 0 2 0.354 1502 TD-2 6 3 0 1 2 0.349 1503 TD-3 6 4 2 0 2 0.345 1504 TD-4 10 0 4 29 10 0.008 1505 TD-5 7 2 2 4 3 0.221 1506 TD-6 11 0 1 1 3 0.268 1507 TD-7 4 1 1 3 2 0.420 1508 AU 6252 9 3 0 0 3 0.302 Rowden 41 1 1 21 16 0.001 M-315 2 1 1 4 2 12 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 1. continued Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. no. Avg.P-value of plants 53 38 55 45 58 62 34 36 44 50 Joe Johnson, PhytoGen Seed Co., LLC, 118 Kennedy Flat Road, Leland, MS 38756 1601 PHY-JJ01 2 6 5 0 3 0.260 1602 PHY-JJ03 9 26 3 1 10 0.006 1603 PHY-JJ05 6 4 2 3 4 0.146 1604 PHY-JJ06 2 5 1 12 5 0.078 1605 PHY-JJ07 4 4 0 4 3 0.221 1606 PHY-JJ08 4 8 1 7 5 0.067 David Weaver, Auburn University, 201 Funchess Hall, Auburn, AL 36849-5415 1607 AU 1221 8 13 1 3 6 0.042 1608 AU 2372 4 3 3 1 3 0.272 Rowden 37 17 11 26 24 < 0.001 M-315 0 5 2 1 2 2010 NATIONAL COTTON FUSARIUM WILT REPORT 13 Table 2. Least squares means of root knot J2 infective stage number (counts per g of root fresh weight), lower and upper 95% confidence intervals, and P-value based on Dunnett’s test versus the resistant check M-315 and susceptible Rowden. Dunnett’s P-value Entry Cultivar/Line Mean Lower Limit Upper Limit M-315 Rowden 0.715 0.264 0.264 0.001 0.264 0.715 0.264 0.136 0.977 † 0.136 † 0.715 † 0.478 0.715 0.470 0.977 0.030 0.030 † 0.001 0.003 0.343 0.030 † 0.023 0.264 0.264 † 0.002 0.066 0.715 0.264 † 0.977 O. Lloyd May, Delta and Pine Land Co., 381 William Gibbs Rd, Tifton, GA 31794 101 LM-1 0 0 4 0.343 102 LM-2 1 0 6 0.030 103 LM-3 1 0 6 0.030 104 LM-4 8 1 21 0.001 105 LM-5 1 0 6 0.030 106 LM-6 0 0 4 0.343 107 LM-7 1 0 6 0.030 108 LM-8 2 0 7 0.009 Rowden 1 0 2 0.023 M-315 0 0 1 Dawn Fraser, Monsanto Company, 741 Coker Farm Road, Hartsville, SC 29550 201 DF-1 0 † † 202 DF-2 2 0 7 203 DF-3 0 † † 204 DF-4 0 0 4 205 DF-5 0 † † 206 DF-6 1 0 9 207 DF-7 0 0 4 208 DF-8 1 0 5 Rowden 1 0 2 M-315 0 0 1 Darren Jones, Monsanto Company, 4600 Old Leyland Road, Leland, MS 38756 301 DJ-1 1 0 6 302 DJ-2 1 0 6 303 DJ-3 0 † † 304 DJ-4 5 1 14 305 DJ-5 2 0 8 306 DJ-6 0 0 4 307 DJ-7 1 0 6 308 DJ-8 0 † † Rowden 1 0 2 M-315 0 0 1 ‡ † 0.009 † 0.343 † 0.171 0.343 0.105 0.023 No rootknot nematode detected 14 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 2. continued Dunnett’s P-value Entry Cultivar/Line Mean Lower Limit Upper Limit M-315 Rowden † 0.032 0.470 † † 0.715 0.715 0.715 0.977 0.470 † † † 0.136 0.715 0.264 0.136 0.977 Dave Albers, Monsanto Company, 800 N Lindbergh Blvd., St. Louis, MO 63167 401 DA-1 0 † † † 402 DA-2 3 0 9 0.001 403 DA-3 1 0 5 0.105 404 DA-4 0 † † † 405 DA-5 0 † † † 406 DA-6 0 0 4 0.343 407 DA-7 0 0 4 0.343 408 DA-8 0 0 4 0.343 Rowden 1 0 2 0.023 M-315 0 0 1 Richard Sheetz, Monsanto Company, 1596 IH 27-87, Hale Center, TX 79041 501 RS-1 1 0 5 502 RS-2 0 † † 503 RS-3 0 † † 504 RS-4 0 † † 505 RS-5 2 0 7 506 RS-6 0 0 4 507 RS-7 1 0 6 508 RS-8 2 0 7 Rowden 1 0 2 M-315 0 0 1 0.105 † † † 0.009 0.343 0.030 0.009 0.023 Daryl Bowman, North Carolina State University, 3709 Hillsborough Street, Raleigh, NC 27607 601 NC1 2 0 7 0.009 0.136 602 NC2 3 0 9 0.001 0.032 603 NC3 1 0 6 0.030 0.264 604 NC4 1 0 5 0.105 0.470 Kathryn Glass, Auburn University, 201 Funchess Hall, Auburn, AL 36849-5415 605 PHY 440W 1 0 6 0.030 David Weaver, Auburn University, 201 Funchess Hall, Auburn, AL 36849-5415 606 AU 6001 2 0 8 607 AU 3111 2 0 7 608 AU 3202 1 0 6 Rowden 1 0 2 M-315 0 0 1 ‡ 0.264 0.066 0.136 0.264 0.977 0.003 0.009 0.030 0.023 No rootknot nematode detected 2010 NATIONAL COTTON FUSARIUM WILT REPORT 15 Table 2. continued Dunnett’s P-value Entry Cultivar/Line Mean Lower Limit Upper Limit M-315 Rowden † 0.715 0.715 0.715 0.715 † 0.977 0.207 0.001 0.015 0.470 0.066 0.264 0.001 0.001 0.977 0.001 0.105 0.001 0.001 † 0.343 0.030 0.009 0.023 0.001 0.470 0.032 0.032 † 0.715 0.264 0.136 0.977 Mustafa McPherson, PhytoGen Seed Co., LLC, 118 Kennedy Flat Road, Leland, MS 38756 701 PHY- MM01 0 † † † 702 PHY- MM02 0 0 4 0.343 703 PHY- MM03 704 PHY- MM04 0 0 4 0.343 705 PHY- MM05 0 0 4 0.343 706 PHY- MM06 0 0 4 0.343 707 PHY- MM07 708 PHY- MM08 0 † † † Rowden 1 0 2 0.023 M-315 0 0 1 Fred Bourland, University of Arkansas, P.O. Box 48, Keiser, AR 72351 801 FB-1 2 0 12 802 FB-2 7 1 19 803 FB-3 3 0 10 804 FB-4 1 0 5 805 FB-5 2 0 8 806 FB-6 1 0 6 807 FB-7 9 1 22 808 FB-8 5 1 15 Rowden 1 0 2 M-315 0 0 1 Brent Styles, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 901 CA-1 9 1 902 CA-2 1 0 903 CA-3 3 0 904 CA-4 3 0 905 CA-5 0 † 906 FS-1 0 0 907 FS-2 1 0 908 FS-3 2 0 Rowden 1 0 M-315 0 0 ‡ 0.032 0.001 0.000 0.105 0.003 0.030 0.001 0.001 0.023 27893 23 5 9 9 † 4 6 7 2 1 No rootknot nematode detected 16 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 2. continued Dunnett’s P-value Entry Cultivar/Line Mean Lower Limit Upper Limit 27893 7 4 † 7 4 † † † 2 1 27893 8 4 11 8 20 11 6 † 2 1 27893 14 6 5 4 4 9 5 † 2 1 M-315 0.009 0.343 † 0.009 0.343 † † † 0.023 Rowden 0.136 0.715 † 0.136 0.715 † † † 0.977 0.003 0.343 0.000 0.003 0.001 0.000 0.030 † 0.023 0.066 0.715 0.007 0.066 0.001 0.007 0.264 † 0.977 0.001 0.030 0.105 0.343 0.343 0.001 0.105 † 0.023 0.002 0.264 0.470 0.715 0.715 0.032 0.470 † 0.977 Brent Styles, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 1001 TP-1 2 0 1002 TP-2 0 0 1003 TP-3 0 † 1004 TP-4 2 0 1005 TP-5 0 0 1006 TP-6 0 † 1007 TP-7 0 † 1008 TP-8 0 † Rowden 1 0 M-315 0 0 Brent Styles, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 1101 TS-1 2 0 1102 TS-2 0 0 1103 TS-3 4 0 1104 TS-4 2 0 1105 FS-4 8 1 1106 FS-5 4 0 1107 MS-9 1 0 1108 AU 6202 0 † Rowden 1 0 M-315 0 0 Brent Styles, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 1201 MS-1 5 1 1202 MS-2 1 0 1203 MS-3 1 0 1204 MS-4 0 0 1205 MS-5 0 0 1206 MS-6 3 0 1207 MS-7 1 0 1208 MS-8 0 † Rowden 1 0 M-315 0 0 ‡ No rootknot nematode detected 2010 NATIONAL COTTON FUSARIUM WILT REPORT 17 Table 2. continued Dunnett’s P-value Entry Cultivar/Line Mean Lower Limit 77905 0 † 0 0 0 0 0 0 0 0 Upper Limit 8 † 4 6 4 6 4 6 2 1 7 6 5 7 21 9 6 † 2 1 M-315 0.003 † 0.343 0.030 0.343 0.030 0.343 0.030 0.023 Rowden 0.066 † 0.715 0.264 0.715 0.264 0.715 0.264 0.977 0.009 0.030 0.105 0.009 0.001 0.001 0.030 † 0.023 0.136 0.264 0.470 0.136 0.001 0.032 0.264 † 0.977 † † 0.470 0.264 0.066 0.470 0.715 † 0.977 Charlie Cook, All-Tex Seed, 356 Hosek Road, Victoria, TX 1301 CC-01 2 1302 CC-02 0 1303 CC-03 0 1304 CC-04 1 1305 CC-05 0 1306 CC-06 1 1307 CC-07 0 1308 CC-08 1 Rowden 1 M-315 0 Cody Poage, All-Tex Seed, P.O. Box 1057, Levelland, TX 79336 1401 CP-01 2 0 1402 CP-02 1 0 1403 CP-03 1 0 1404 CP-04 2 0 1405 CP-05 8 1 1406 CP-06 3 0 1407 CP-07 1 0 1408 CP-08 0 † Rowden 1 0 M-315 0 0 Tim Dabbert, Monsanto Company, 38768 W. Farrell Road, Maricopa, AZ 85138 1501 TD-1 0 † † † 1502 TD-2 0 † † † 1503 TD-3 1 0 5 0.105 1504 TD-4 1 0 6 0.030 1505 TD-5 2 0 8 0.003 1506 TD-6 1 0 5 0.105 1507 TD-7 0 0 4 0.343 1508 AU 6252 0 † † † Rowden 1 0 2 0.023 M-315 0 0 1 ‡ No rootknot nematode detected 18 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 2. continued Dunnett’s P-value Entry Cultivar/Line Mean Lower Limit Upper Limit M-315 Rowden † 0.470 0.136 0.004 0.264 † 0.032 † 0.977 Joe Johnson, PhytoGen Seed Co., LLC, 118 Kennedy Flat Road, Leland, MS 38756 1601 PHY-JJ01 0 † † † 1602 PHY-JJ03 1 0 5 0.105 1603 PHY-JJ05 2 0 7 0.009 1604 PHY-JJ06 4 0 13 0.001 1605 PHY-JJ07 1 0 6 0.030 1606 PHY-JJ08 0 † † † David Weaver, Auburn University, 201 Funchess Hall, Auburn, AL 36849-5415 1607 AU 1221 3 0 9 0.001 1608 AU 2372 0 † † † Rowden 1 0 2 0.023 M-315 0 0 1 ‡ No rootknot nematode detected 2010 NATIONAL COTTON FUSARIUM WILT REPORT 19 Table 3. Least squares means of root knot nematode egg number (count per g root fresh weight), lower and upper 95% confidence intervals, and P-value based on Dunnett’s versus the resistant check M-315 and susceptible Rowden. Dunnett’s P-value Entry Cultivar/Line Mean Lower Limit Upper Limit M-315 Rowden 0.915 0.963 0.815 0.664 0.998 0.733 0.675 0.956 1.000 0.352 0.467 0.998 0.177 0.408 0.863 0.915 0.687 1.000 0.001 0.023 0.067 0.002 0.243 0.026 0.003 0.043 0.001 0.740 0.979 0.992 0.871 0.998 0.981 0.889 0.988 1.000 O. Lloyd May, Delta and Pine Land Co., 381 William Gibbs Rd, Tifton, GA 31794 101 LM-1 10 1 31 0.004 102 LM-2 7 1 24 0.012 103 LM-3 13 1 42 0.001 104 LM-4 18 2 56 0.000 105 LM-5 3 -0 10 0.191 106 LM-6 14 1 70 0.009 107 LM-7 17 2 55 0.000 108 LM-8 8 1 25 0.010 Rowden 23 3 35 0.001 M-315 2 -0 3 Dawn Fraser, Monsanto Company, 741 Coker Farm Road, Hartsville, SC 29550 201 DF-1 31 2 155 202 DF-2 24 2 75 203 DF-3 3 -0 10 204 DF-4 39 3 123 205 DF-5 27 2 138 206 DF-6 9 1 48 207 DF-7 10 1 31 208 DF-8 17 2 54 Rowden 23 3 35 M-315 2 -0 3 Darren Jones, Monsanto Company, 4600 Old Leyland Road, Leland, MS 38756 301 DJ-1 15 2 49 302 DJ-2 6 1 20 303 DJ-3 4 0 15 304 DJ-4 11 1 37 305 DJ-5 3 -0 9 306 DJ-6 6 1 19 307 DJ-7 11 1 35 308 DJ-8 5 0 17 Rowden 23 3 35 M-315 2 -0 3 0.001 0.000 0.201 0.001 0.001 0.026 0.004 0.000 0.001 20 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 3. continued Dunnett’s P-value Entry Cultivar/Line Mean Lower Limit Upper Limit M-315 Rowden 0.985 0.244 0.918 0.996 0.983 0.345 0.862 0.999 1.000 0.991 0.984 0.992 0.731 0.591 0.984 0.917 0.413 1.000 Dave Albers, Monsanto Company, 800 N Lindbergh Blvd., St. Louis, MO 63167 401 DA-1 5 0 18 0.034 402 DA-2 34 2 107 0.001 403 DA-3 7 0 37 0.051 404 DA-4 0 -4 7 0.828 405 DA-5 6 1 19 0.030 406 DA-6 29 2 90 0.001 407 DA-7 12 1 38 0.002 408 DA-8 2 -1 7 0.415 Rowden 23 3 35 0.001 M-315 2 -0 3 Richard Sheetz, Monsanto Company, 1596 IH 27-87, Hale Center, TX 501 RS-1 5 0 502 RS-2 5 0 503 RS-3 4 0 504 RS-4 16 2 505 RS-5 20 2 506 RS-6 6 0 507 RS-7 10 1 508 RS-8 26 2 Rowden 23 3 M-315 2 -0 79041 16 18 15 50 63 19 31 81 35 3 0.056 0.032 0.068 0.001 0.000 0.031 0.004 0.001 0.001 Daryl Bowman, North Carolina State University, 3709 Hillsborough Street, Raleigh, NC 27607 601 NC1 8 0 45 0.034 0.883 602 NC2 10 1 54 0.021 0.837 603 NC3 18 1 93 0.005 0.620 604 NC4 6 0 33 0.075 0.938 Kathryn Glass, Auburn University, 201 Funchess Hall, Auburn, AL 36849-5415 605 PHY 440W 30 2 156 0.001 David Weaver, Auburn University, 201 Funchess Hall, Auburn, AL 36849-5415 606 AU 6001 32 2 166 607 AU 3111 15 1 78 608 AU 3202 26 2 135 Rowden 23 3 35 M-315 2 -0 3 0.001 0.007 0.002 0.001 0.365 0.336 0.700 0.436 1.000 2010 NATIONAL COTTON FUSARIUM WILT REPORT 21 Table 3. continued Dunnett’s P-value Entry Cultivar/Line Mean Lower Limit Upper Limit M-315 Rowden 0.936 0.998 0.734 1.000 0.999 0.999 1.000 0.877 0.672 0.971 0.856 0.669 0.944 0.251 0.902 1.000 0.001 0.257 0.001 0.000 0.006 0.005 0.001 0.001 0.001 0.135 0.999 0.404 0.569 0.933 0.928 0.743 0.774 1.000 Mustafa McPherson, PhytoGen Seed Co., LLC, 118 Kennedy Flat Road, Leland, MS 38756 701 PHY- MM01 6 0 33 0.069 702 PHY- MM02 3 -0 10 0.197 703 PHY- MM03 704 PHY- MM04 16 2 50 0.001 705 PHY- MM05 1 -1 5 0.656 706 PHY- MM06 2 -1 8 0.328 707 PHY- MM07 708 PHY- MM08 2 -1 7 0.426 Rowden 23 3 35 0.001 M-315 2 -0 3 Fred Bourland, University of Arkansas, P.O. Box 48, Keiser, AR 72351 801 FB-1 11 1 36 802 FB-2 17 2 55 803 FB-3 7 1 22 804 FB-4 12 1 38 805 FB-5 18 2 56 806 FB-6 8 1 27 807 FB-7 34 2 106 808 FB-8 10 1 33 Rowden 23 3 35 M-315 2 -0 3 Brent Styles, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 27893 901 CA-1 44 3 137 902 CA-2 2 -0 9 903 CA-3 26 2 82 904 CA-4 20 2 65 905 CA-5 9 1 29 906 FS-1 9 1 30 907 FS-2 15 2 49 908 FS-3 14 2 46 Rowden 23 3 35 M-315 2 -0 3 0.002 0.000 0.016 0.002 0.000 0.007 0.001 0.003 0.001 22 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 3. continued Dunnett’s P-value Entry Cultivar/Line Mean Lower Limit Upper Limit 27893 56 9 9 18 25 6 14 7 35 3 M-315 0.000 0.281 0.279 0.033 0.010 0.531 0.085 0.488 0.001 Rowden 0.666 0.999 0.999 0.985 0.958 1.000 0.994 1.000 1.000 0.002 0.000 0.047 0.000 0.000 0.001 0.003 0.018 0.001 0.862 0.614 0.989 0.654 0.526 0.227 0.902 0.974 1.000 0.001 0.000 0.002 0.167 0.032 0.001 0.031 0.001 0.001 0.086 0.601 0.865 0.997 0.984 0.718 0.984 0.837 1.000 Brent Styles, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 1001 TP-1 18 2 1002 TP-2 2 -0 1003 TP-3 2 -0 1004 TP-4 5 0 1005 TP-5 8 1 1006 TP-6 1 -1 1007 TP-7 4 0 1008 TP-8 2 -1 Rowden 23 3 M-315 2 -0 Brent Styles, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 27893 1101 TS-1 12 1 38 1102 TS-2 19 2 61 1103 TS-3 5 0 16 1104 TS-4 18 2 57 1105 FS-4 22 2 69 1106 FS-5 35 2 111 1107 MS-9 10 1 33 1108 AU 6202 6 1 21 Rowden 23 3 35 M-315 2 -0 3 Brent Styles, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 27893 1201 MS-1 52 3 161 1202 MS-2 20 2 62 1203 MS-3 12 1 37 1204 MS-4 3 -0 11 1205 MS-5 5 0 18 1206 MS-6 16 2 51 1207 MS-7 6 0 19 1208 MS-8 12 1 40 Rowden 23 3 35 M-315 2 -0 3 2010 NATIONAL COTTON FUSARIUM WILT REPORT 23 Table 3. continued Dunnett’s P-value Entry Cultivar/Line Mean Lower Limit 77905 1 -0 1 1 2 2 -1 2 3 -0 Upper Limit 41 12 31 36 53 101 5 55 35 3 90 40 20 38 90 36 13 6 35 3 M-315 0.001 0.126 0.004 0.002 0.000 0.001 0.650 0.000 0.001 Rowden 0.831 0.996 0.919 0.879 0.697 0.279 1.000 0.673 1.000 0.001 0.002 0.023 0.002 0.001 0.002 0.095 0.512 0.001 0.349 0.843 0.979 0.858 0.349 0.876 0.995 1.000 1.000 0.975 0.989 0.998 0.901 0.754 0.883 0.859 0.994 1.000 Charlie Cook, All-Tex Seed, 356 Hosek Road, Victoria, TX 1301 CC-01 13 1302 CC-02 3 1303 CC-03 10 1304 CC-04 11 1305 CC-05 17 1306 CC-06 32 1307 CC-07 1 1308 CC-08 17 Rowden 23 M-315 2 Cody Poage, All-Tex Seed, P.O. Box 1057, Levelland, TX 79336 1401 CP-01 29 2 1402 CP-02 12 1 1403 CP-03 6 1 1404 CP-04 12 1 1405 CP-05 29 2 1406 CP-06 11 1 1407 CP-07 4 0 1408 CP-08 2 -1 Rowden 23 3 M-315 2 -0 Tim Dabbert, Monsanto Company, 38768 W. Farrell Road, Maricopa, AZ 85138 1501 TD-1 6 1 21 0.019 1502 TD-2 5 0 16 0.048 1503 TD-3 3 -0 11 0.182 1504 TD-4 10 1 33 0.003 1505 TD-5 15 2 48 0.001 1506 TD-6 11 1 35 0.003 1507 TD-7 12 1 38 0.002 1508 AU 6252 4 0 14 0.087 Rowden 23 3 35 0.001 M-315 2 -0 3 24 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 3. continued Dunnett’s P-value Entry Cultivar/Line Mean Lower Limit Upper Limit M-315 Rowden 0.963 0.570 0.708 0.449 0.902 0.866 0.866 0.712 1.000 Joe Johnson, PhytoGen Seed Co., LLC, 118 Kennedy Flat Road, Leland, MS 38756 1601 PHY-JJ01 4 -0 26 0.131 1602 PHY-JJ03 20 1 103 0.003 1603 PHY-JJ05 14 1 77 0.008 1604 PHY-JJ06 25 2 131 0.002 1605 PHY-JJ07 7 0 41 0.043 1606 PHY-JJ08 9 0 48 0.028 David Weaver, Auburn University, 201 Funchess Hall, Auburn, AL 36849-5415 1607 AU 1221 9 0 48 0.028 1608 AU 2372 12 0 128 0.049 Rowden 23 3 35 0.001 M-315 2 -0 3 2010 NATIONAL COTTON FUSARIUM WILT REPORT 25 Table 4. Least squares means of total root knot nematode number (count per plant), lower and upper 95% confidence intervals, and P-value based on Dunnett’s versus the resistant check M-315 and susceptible Rowden. Dunnett’s P-value Entry Cultivar/Line Mean Lower Limit Upper Limit M-315 Rowden 0.888 0.743 0.554 0.345 0.914 0.824 0.817 0.575 1.000 0.482 0.926 1.000 0.668 0.658 0.959 0.989 0.913 O. Lloyd May, Delta and Pine Land Co., 381 William Gibbs Rd, Tifton, GA 31794 101 LM-1 43 2 292 0.068 102 LM-2 75 2 508 0.024 103 LM-3 123 3 828 0.009 104 LM-4 203 3 1360 0.003 105 LM-5 36 2 249 0.091 106 LM-6 57 2 389 0.040 107 LM-7 59 2 399 0.038 108 LM-8 117 3 788 0.010 Rowden 140 4 261 0.001 M-315 10 2 19 Dawn Fraser, Monsanto Company, 741 Coker Farm Road, Hartsville, SC 29550 201 DF-1 144 4 509 202 DF-2 67 3 164 203 DF-3 4 1 12 204 DF-4 113 4 277 205 DF-5 109 3 386 206 DF-6 43 2 153 207 DF-7 39 3 97 208 DF-8 70 3 173 Rowden 140 4 261 M-315 Darren Jones, Monsanto Company, 4600 Old Leyland Road, Leland, MS 38756 301 DJ-1 103 3 695 302 DJ-2 31 2 216 303 DJ-3 31 2 216 304 DJ-4 126 3 848 305 DJ-5 33 2 229 306 DJ-6 38 2 262 307 DJ-7 53 2 359 308 DJ-8 11 0 80 Rowden 140 4 261 M-315 10 2 19 0.001 0.001 0.922 0.000 0.002 0.019 0.008 0.001 0.001 0.013 0.116 0.116 0.008 0.105 0.083 0.046 0.452 0.001 0.627 0.933 0.933 0.543 0.926 0.906 0.844 0.990 1.000 26 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 4. continued Dunnett’s P-value Entry Cultivar/Line Mean Lower Limit Upper Limit M-315 Rowden 0.914 0.358 0.944 0.984 0.962 0.411 0.879 0.990 1.000 0.933 0.954 0.966 0.935 0.520 0.957 0.890 0.799 1.000 Dave Albers, Monsanto Company, 800 N Lindbergh Blvd., St. Louis, MO 63167 401 DA-1 36 2 249 0.091 402 DA-2 197 3 1317 0.003 403 DA-3 28 1 196 0.137 404 DA-4 5 -1 94 0.683 405 DA-5 23 1 159 0.189 406 DA-6 173 3 1157 0.004 407 DA-7 45 2 306 0.063 408 DA-8 11 0 80 0.452 Rowden 140 4 261 0.001 M-315 10 2 19 Richard Sheetz, Monsanto Company, 1596 IH 27-87, Hale Center, TX 79041 501 RS-1 31 2 216 502 RS-2 25 1 176 503 RS-3 21 1 149 504 RS-4 31 2 213 505 RS-5 133 3 894 506 RS-6 24 1 169 507 RS-7 42 2 289 508 RS-8 63 2 425 Rowden 140 4 261 M-315 10 2 19 0.116 0.162 0.208 0.119 0.008 0.172 0.070 0.033 0.001 Daryl Bowman, North Carolina State University, 3709 Hillsborough Street, Raleigh, NC 27607 601 NC1 48 2 329 0.055 0.864 602 NC2 72 2 489 0.026 0.757 603 NC3 36 2 246 0.093 0.916 604 NC4 13 1 96 0.370 0.985 Kathryn Glass, Auburn University, 201 Funchess Hall, Auburn, AL 36849-5415 605 PHY 440W 107 3 718 0.012 David Weaver, Auburn University, 201 Funchess Hall, Auburn, AL 36849-5415 606 AU 6001 89 3 602 607 AU 3111 83 3 558 608 AU 3202 122 3 818 Rowden 140 4 261 M-315 10 2 19 0.017 0.020 0.009 0.001 0.613 0.683 0.711 0.559 1.000 2010 NATIONAL COTTON FUSARIUM WILT REPORT 27 Table 4. continued Dunnett’s P-value Entry Cultivar/Line Mean Lower Limit Upper Limit M-315 Rowden 0.797 0.975 0.813 0.995 0.983 0.992 1.000 0.577 0.130 0.922 0.668 0.123 0.786 0.021 0.619 1.000 0.000 0.172 0.003 0.005 0.009 0.137 0.003 0.014 0.001 0.069 0.957 0.333 0.442 0.557 0.944 0.339 0.650 1.000 Mustafa McPherson, PhytoGen Seed Co., LLC, 118 Kennedy Flat Road, Leland, MS 38756 701 PHY- MM01 47 1 705 0.121 702 PHY- MM02 18 1 126 0.264 703 PHY- MM03 704 PHY- MM04 60 2 405 0.037 705 PHY- MM05 7 -0 53 0.632 706 PHY- MM06 14 1 103 0.342 707 PHY- MM07 708 PHY- MM08 9 0 70 0.512 Rowden 140 4 261 0.001 M-315 10 2 19 Fred Bourland, University of Arkansas, P.O. Box 48, Keiser, AR 72351 801 FB-1 127 4 311 802 FB-2 245 5 597 803 FB-3 68 3 167 804 FB-4 113 4 277 805 FB-5 249 5 608 806 FB-6 95 4 232 807 FB-7 416 5 1014 808 FB-8 121 4 296 Rowden 140 4 261 M-315 10 2 19 Brent Styles, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 27893 901 CA-1 599 4 3994 902 CA-2 24 1 169 903 CA-3 210 3 1403 904 CA-4 160 3 1074 905 CA-5 122 3 821 906 FS-1 28 1 196 907 FS-2 206 3 1380 908 FS-3 97 3 655 Rowden 140 4 261 M-315 10 2 19 0.000 0.001 0.001 0.000 0.001 0.000 0.001 0.000 0.001 28 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 4. continued Dunnett’s P-value Entry Cultivar/Line Mean Lower Limit Upper Limit M-315 0.011 0.452 0.274 0.062 0.162 0.471 0.659 0.400 0.001 Rowden 0.596 0.990 0.977 0.877 0.954 0.991 0.996 0.987 1.000 0.005 0.019 0.040 0.004 0.003 0.002 0.017 0.070 0.001 0.447 0.702 0.824 0.380 0.356 0.255 0.687 0.890 1.000 0.001 0.016 0.009 0.195 0.305 0.011 0.129 0.026 0.001 0.160 0.679 0.562 0.963 0.980 0.608 0.941 0.757 1.000 Brent Styles, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 27893 1001 TP-1 111 3 748 1002 TP-2 11 0 80 1003 TP-3 17 1 123 1004 TP-4 45 2 309 1005 TP-5 25 1 176 1006 TP-6 10 0 76 1007 TP-7 6 -0 50 1008 TP-8 12 1 90 Rowden 140 4 261 M-315 10 2 19 Brent Styles, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 27893 1101 TS-1 158 3 1061 1102 TS-2 85 3 572 1103 TS-3 57 2 389 1104 TS-4 186 3 1247 1105 FS-4 198 3 1323 1106 FS-5 260 4 1736 1107 MS-9 88 3 595 1108 AU 6202 42 2 289 Rowden 140 4 261 M-315 10 2 19 Brent Styles, Bayer Crop Science, 4205 Williamson Road, Wilson, NC 27893 1201 MS-1 360 4 2404 1202 MS-2 90 3 608 1203 MS-3 121 3 811 1204 MS-4 22 1 156 1205 MS-5 16 1 113 1206 MS-6 108 3 728 1207 MS-7 29 1 203 1208 MS-8 72 2 489 Rowden 140 4 261 M-315 10 2 19 2010 NATIONAL COTTON FUSARIUM WILT REPORT 29 Table 4. continued Dunnett’s P-value Entry Cultivar/Line Mean Lower Limit Upper Limit 399 269 395 179 395 1021 30 1160 261 19 3734 788 389 781 2690 1390 266 123 261 19 M-315 0.038 0.079 0.039 0.158 0.039 0.006 0.832 0.004 0.001 Rowden 0.817 0.902 0.820 0.953 0.820 0.464 0.998 0.409 1.000 0.000 0.010 0.040 0.010 0.001 0.003 0.081 0.274 0.001 0.077 0.575 0.824 0.578 0.135 0.336 0.904 0.977 1.000 0.962 0.989 0.954 0.853 0.844 0.968 0.914 0.984 1.000 Charlie Cook, All-Tex Seed, 356 Hosek Road, Victoria, TX 77905 1301 CC-01 59 2 1302 CC-02 39 2 1303 CC-03 58 2 1304 CC-04 26 1 1305 CC-05 58 2 1306 CC-06 152 3 1307 CC-07 4 -1 1308 CC-08 173 3 Rowden 140 4 M-315 10 2 Cody Poage, All-Tex Seed, P.O. Box 1057, Levelland, TX 79336 1401 CP-01 560 4 1402 CP-02 117 3 1403 CP-03 57 2 1404 CP-04 117 3 1405 CP-05 404 4 1406 CP-06 208 3 1407 CP-07 39 2 1408 CP-08 18 1 Rowden 140 4 M-315 10 2 Tim Dabbert, Monsanto Company, 38768 W. Farrell Road, Maricopa, AZ 85138 1501 TD-1 23 1 159 0.189 1502 TD-2 12 0 83 0.434 1503 TD-3 26 1 176 0.162 1504 TD-4 51 2 346 0.050 1505 TD-5 53 2 359 0.046 1506 TD-6 21 1 146 0.215 1507 TD-7 37 2 249 0.091 1508 AU 6252 14 1 100 0.356 Rowden 140 4 261 0.001 M-315 10 2 19 30 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 4. continued Dunnett’s P-value Entry Cultivar/Line Mean Lower Limit Upper Limit M-315 Rowden 0.980 0.759 0.619 0.667 0.873 0.941 0.573 0.866 1.000 Joe Johnson, PhytoGen Seed Co., LLC, 118 Kennedy Flat Road, Leland, MS 38756 1601 PHY-JJ01 16 1 113 0.305 1602 PHY-JJ03 72 2 485 0.026 1603 PHY-JJ05 106 3 708 0.012 1604 PHY-JJ06 94 3 628 0.015 1605 PHY-JJ07 47 2 316 0.059 1606 PHY-JJ08 30 1 203 0.129 David Weaver, Auburn University, 201 Funchess Hall, Auburn, AL 36849-5415 1607 AU 1221 118 3 791 0.010 1608 AU 2372 48 2 326 0.056 Rowden 140 4 261 0.001 M-315 10 2 19 2010 NATIONAL COTTON FUSARIUM WILT REPORT 31 Commercial Cotton Wilt Trial 95 % Confidence Limit Dunnett’s P-value vs. Rowden 0.378 0.013 0.011 0.019 0.001 0.011 0.006 0.065 0.004 0.001 M-315 0.044 0.326 0.172 0.080 0.790 0.335 0.207 0.046 0.188 0.560 0.005 0.013 Table 5. Least squares estimates of the average percent wilted plants for entries and checks, confidence intervals, and P-values based on Dunnett’s versus the susceptible check Rowden and the resistant check Cultivar DP 0949 DP 1028 DP 1050 FM 1740 PHY 367 PHY 375 PHY 485 PHY 565 STV 4288 STV 5458 Rowden M-315 Mean 7.6 2.6 3.7 5.4 1.1 2.5 3.3 6.9 3.4 1.6 16.3 0.4 Lower 3.7 0.9 1.6 2.9 0.3 0.9 1.4 4.1 1.6 0.6 11.4 0.0 Upper 15.1 7.2 8.1 9.7 3.6 7.1 7.4 11.5 7.2 4.6 23.0 3.6 32 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 6. Least squares estimate of root knot J2 infective stage number (counts per g of root fresh weight) for entries and checks, confidence intervals, and P-values based on Dunnett’s test versus the susceptible check Rowden and the resistant check M-315. 95 % Confidence Limit Cultivar DP 0949 DP 1028 DP 1050 FM 1740 PHY 367 PHY 375 PHY 485 PHY 565 STV 4288 STV 5458 Rowden M-315 Mean 1176 1768 2316 1804 3467 1808 1535 3133 2603 2784 1151 2777 Lower 486 1078 1626 1114 2776 1118 845 2442 1912 2094 460 2087 Upper 1866 2458 3006 2494 4157 2498 2226 3823 3293 3474 1841 3467 0.017 Dunnett’s P-value vs. Rowden 1.000 0.793 0.150 0.743 0.000 0.738 0.985 0.003 0.042 0.017 M-315 0.020 0.272 0.951 0.309 0.691 0.313 0.109 0.991 1.000 1.000 0.017 2010 NATIONAL COTTON FUSARIUM WILT REPORT 33 Table 7. Least squares estimate of root knot number (counts per 150 cc) for entries and checks, confidence intervals, and P-values based on Dunnett’s versus the susceptible check Rowden and the resistant check M-315. 95 % Confidence Limit Cultivar DP 0949 DP 1028 DP 1050 FM 1740 PHY 367 PHY 375 PHY 485† PHY 565 STV 4288 STV 5458 Rowden M-315 † Dunnett’s P-value vs. Rowden 0.973 1.000 1.000 0.680 0.791 1.000 0.376 0.112 1.000 1.000 M-315 0.973 1.000 1.000 0.680 0.791 1.000 0.376 0.112 1.000 1.000 1.000 1.000 Mean 174 97 116 290 251 97 695 773 77 77 77 77 Lower 52 19 27 114 92 19 47 437 13 13 13 13 Upper 578 484 504 734 682 484 10386 1366 468 468 468 468 Observations for PHY 485 were highly variable across the test, hence the large upper confidence limit.. 34 ALABAMA AGRICULTURAL EXPERIMENT STATION Table 8. Least squares estimate of root knot egg number (counts per g of root fresh weight) for entries and checks, confidence intervals, and P-values based on Dunnett’s versus the susceptible check Rowden and the resistant check M-315. 95 % Confidence Limit Cultivar DP 0949 DP 1028 DP 1050 FM 1740 PHY 367 PHY 375 PHY 485 PHY 565 STV 4288 STV 5458 Rowden M-315 Mean 775 1092 693 1852 382 1187 1170 1585 893 901 2149 88 Lower 347 488 310 1176 171 531 524 1006 567 403 962 39 Upper 1732 2440 1550 2916 853 2652 2616 2495 1406 2013 4801 197 <.0001 Dunnett’s P-value vs. Rowden 0.318 0.734 0.225 1.000 0.023 0.835 0.819 0.980 0.217 0.484 M-315 0.003 0.001 0.005 0.001 0.067 0.001 0.001 0.001 0.001 0.002 0.001 2010 NATIONAL COTTON FUSARIUM WILT REPORT 35 Table 9. Least squares estimate of seed cotton yield (lbs per acre) for entries and checks, confidence intervals, and P-values based on Dunnett’s versus the susceptible check Rowden and the resistant check M-315. 95 % Confidence Limit Cultivar DP 0949 DP 1028 DP 1050 FM 1740 PHY 367 PHY 375 PHY 485 PHY 565 STV 4288 STV 5458 Rowden M-315 Mean 1176 1768 2316 1804 3467 1808 1535 3133 2603 2784 1151 2777 Lower 486 1078 1626 1114 2776 1118 845 2442 1912 2094 460 2087 Upper 1866 2458 3006 2494 4157 2498 2226 3823 3293 3474 1841 3467 0.017 Dunnett’s P-value vs. Rowden 1.000 0.793 0.150 0.743 0.000 0.738 0.985 0.003 0.042 0.017 M-315 0.020 0.272 0.951 0.309 0.691 0.313 0.109 0.991 1.000 1.000 0.017