2005 National Cotton Fusarium Wilt Report December 2005 AGRONOMY AND SOILS DEPARTMENT SERIES NO. 271 ALABAMA AGRICULTURAL EXPERIMENT STATION RICHARD GUTHRIE, DIRECTOR AUBURN UNIVERSITY AUBURN, AL 36849 THIS REPORT IS A JOINT CONTRIBUTION BETWEEN USDA-ARS, CROP SCIENCE RESEARCH LABORATORY, MISSISSIPPI STATE UNIVERSITY, MISSISSIPPI, AND THE ALABAMA AGRICULTURAL EXPERIMENT STATION, AUBURN UNIVERSITY, ALABAMA Information contained herein is available to all persons regardless of race, color, sex, or national origin 2005 NATIONAL COTTON FUSARIUM WILT REPORT 3 2005 NATIONAL COTTON FUSARIUM WILT REPORT Kathryn M. Glass1, William S. Gazaway2, and Edzard van Santen 3 1,3 Agricultural Program Associate and Professor, respectively, Dept. of Agronomy and Soils, Auburn University, AL 36849 2 Professor Emeritus and Extension Pathologist/Nematologist, Dept. of Entomology and Plant Pathology, Auburn University, 36849 Cotton cultivars and elite breeding lines submitted by 28 cooperators were evaluated for Fusarium wilt resistance under field conditions at the E. V Smith Research Center, Plant Breeding Unit, . Tallassee, Alabama. These entries were grown on an Independence loamy fine sand highly infested with the Fusarium wilt fungus (Fusarium oxysporum) Schlect. f. vasinfectum [Atk.] (Snyd. & Hans.) and southern root-knot nematodes (Meloidogyne incognita.). In 1994, a soil analysis for nematodes revealed that southern root-knot (Meloidogyne incognita) and lance (Hoplolaimus galeatus) are the predominant nematode species in the test plots. High populations of both species are found throughout the test area. Other nematode genera present are stubby root (Trichodorus sp.) and stunt (Tylenchorhynchus sp.). Root-knot nematodes, however, appear to be causing the major damage to cotton in the Fusarium Wilt Test as indicated by the high galling indices found on the roots of all cotton lines. Entries were planted in single 20-foot rows on 40-inch centers, separated by 5-foot alleys. Four replications of the test entries and checks were evaluated in a randomized complete block design with a split plot restriction on randomization. The set of eight test cultivars submitted by a cooperator was always evaluated as a group together with two control plots within each replicate. Both susceptible (Rowden) and resistant (M-315) cultivars were included as check subplots in the two center rows of each main plot (Fig. 1). Plots were planted June 3. Extreme dry conditions delayed planting and once planted, excessive moisture was a problem. Initial plant counts were made on June 24. Wilted plants were counted and removed on July 21, August 3, and August 25. The remaining live plants were counted and recorded on September 15, delayed due to excessive rainfall. Total percent wilted plants were then determined and mean wilting for a given entry calculated. The average % wilted plants for the susceptible check Rowden was 56%, with a range from 19 to 88% on an individual plot basis (Fig. 1). Wilt development was quite uniform in all blocks with rep avearges ranging from 49 to 64%. The resistant check M-315 had erratic emergence due to problems caused by chemical delinting and thus was not included in this report. Critical evaluations of breeding lines should be made relative to the Rowden check listed at the bottom of each group. 4 ALABAMA AGRICULTURAL EXPERIMENT STATION Fig. 1. Field plot layout and % wilt for control plot of Rowden (suceptible) and M-315 (resistant). Distances (ft) from the SE corner of the trial are given in the left hand column and the bottom row. EW 450 425 400 375 350 325 300 275 250 37 M-315 Rowden 56 Rowden M-315 38 Rowden M-315 49 M-315 Rowden 67 M-315 Rowden 55 M-315 Rowden 62 Rowden M-315 73 Rowden M-315 72 Rowden M-315 75 M-315 Rowden 42 M-315 Rowden 65 Rowden M-315 54 M-315 Rowden 41 Rowden M-315 71 M-315 Rowden 71 Rowden M-315 48 M-315 Rowden 66 M-315 Rowden 73 Rowden M-315 68 Rowden M-315 M-315 M-315 M-315 80 Rowden 58 Rowden 47 Rowden 27 M-315 Rowden 81 Rowden M-315 69 M-315 Rowden 85 M-315 Rowden 72 Rowden M-315 83 Rowden M-315 68 M-315 Rowden 53 Rowden M-315 48 M-315 Rowden 27 M-315 Rowden 51 M-315 Rowden 32 M-315 Rowden 65 Rowden M-315 49 M-315 Rowden 54 M-315 Rowden 40 M-315 Rowden 69 Rowden M-315 33 M-315 Rowden 56 M-315 Rowden 61 M-315 Rowden 65 Rowden M-315 86 M-315 Rowden 70 M-315 Rowden 46 Rowden M-315 79 M-315 Rowden 85 Rowden M-315 75 Rowden M-315 69 Rowden M-315 64 Rowden M-315 8 rows 8 rows 8 rows 8 rows 8 rows 225 200 175 150 125 100 75 50 25 27 M-315 Rowden 28 M-315 Rowden 21 M-315 Rowden 29 Rowden M-315 58 Rowden M-315 51 Rowden M-315 59 Rowden M-315 48 M-315 Rowden 63 M-315 Rowden 40 Rowden M-315 54 Rowden M-315 53 M-315 Rowden 65 Rowden M-315 36 Rowden M-315 63 Rowden M-315 61 Rowden M-315 55 Rowden M-315 81 M-315 Rowden 66 Rowden M-315 60 76 Rowden M-315 57 Rowden M-315 80 Rowden M-315 52 Rowden M-315 M-315 46 Rowden 56 Rowden M-315 77 M-315 Rowden 64 M-315 Rowden 43 Rowden M-315 67 M-315 Rowden 72 Rowden M-315 80 M-315 Rowden 50 72 M-315 Rowden 64 Rowden M-315 29 Rowden M-315 127 70 Rowden M-315 42 M-315 Rowden 33 M-315 Rowden 24 M-315 Rowden 19 M-315 Rowden 25 M-315 Rowden 26 M-315 Rowden 35 Rowden M-315 45 M-315 Rowden 46 M-315 Rowden 160 8 rows 40 Rowden M-315 80 M-315 Rowden 71 M-315 Rowden 64 M-315 Rowden 88 M-315 Rowden 24 Rowden M-315 59 M-315 Rowden 44 Rowden M-315 53 M-315 Rowden 34 Rowden M-315 193 73 Rowden M-315 M-315 46 Rowden 68 Rowden M-315 64 Rowden M-315 M-315 93 60 Rowden 60 0 M-315 Rowden NS==> 27 N The resistant check M-315 had erratic emergence due to problems related to chemical delinting. Results for this entry are thus omitted from the report. 2004 NATIONAL COTTON FUSARIUM WILT REPORT 5 2005 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. P-value Gary L. Rea, Delta and Pine Land Co., 247 US HWY 380 W, Haskell, TX 79521 101 GLR-1 9 3 6 4 5 0.295 102 GLR-2 1 1 1 1 1 0.816 103 GLR-3 30 3 7 0 10 0.064 104 GLR-4 1 6 11 5 6 0.267 105 GLR-5 8 41 8 3 15 0.007 106 GLR-6 11 7 3 10 8 0.136 107 GLR-7 0 0 1 0 0 0.958 108 GLR-8 2 14 6 2 6 0.235 109 Rowden 46 25 75 67 53 <0.001 110 M-315 Not included because of emergence problems Johnie Jenkins, USDA-ARS, Mississippi State, MS 39762 201 JNJ-1 3 3 1 1 2 0.617 202 JNJ-2 7 4 6 5 5 0.197 203 JNJ-3 7 4 3 2 4 0.327 204 JNJ-4 12 10 11 1 9 0.042 205 JNJ-5 6 6 3 4 5 0.257 206 JNJ-6 0 0 3 0 1 0.878 207 JNJ-7 4 19 7 4 8 0.051 208 JNJ-8 7 15 23 1 12 0.009 209 Rowden 73 21 40 58 48 <0.001 Not included because of emergence problems 210 M-315 Jack McCarty, USDA, Mississippi State, MS 39762 301 JCM-1 0 4 9 1 4 0.144 302 JCM-2 1 2 6 1 3 0.246 303 JCM-3 8 8 3 0 5 0.064 304 JCM-4 0 18 3 5 6 0.010 305 JCM-5 1 6 1 3 3 0.244 306 JCM-6 9 2 3 0 3 0.164 307 JCM-7 14 3 9 0 7 0.009 308 JCM-8 10 13 3 6 8 0.002 309 Rowden 60 67 69 56 63 <0.001 Not included because of emergence problems 310 M-315 continued THE P-VALUE INDICATES THE PROBABILITY THAT THE AVERAGE WILT PERCENTAGE DIFFERS FROM ZERO. 6 ALABAMA AGRICULTURAL EXPERIMENT STATION 2005 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. P-value Russ Hayes, USDA, Mississippi State, MS 39762 401 RH-1 14 11 9 0 8 0.044 402 RH-2 4 4 1 6 4 0.371 403 RH-3 2 3 3 2 2 0.544 404 RH-4 25 26 23 24 24 <0.001 405 RH-5 4 19 4 18 11 0.009 406 RH-6 21 11 3 1 9 0.030 407 RH-7 6 10 12 15 11 0.013 408 RH-8 4 12 2 10 7 0.094 409 Rowden 45 43 76 80 61 <0.001 Not included because of emergence problems 410 M-315 Laval Verhalen, Oklahoma State University, 368 Agricultural Hall, Stillwater, OK 74078 501 OKLA-1 18 11 4 1 9 0.280 502 OKLA-2 5 2 6 26 10 0.230 503 OKLA-3 3 11 2 4 5 0.546 504 OKLA-4 15 6 16 17 14 0.093 505 OKLA-5 12 10 2 11 9 0.281 506 OKLA-6 20 22 24 27 23 0.006 507 OKLA-7 2 28 75 6 28 0.001 508 OKLA-8 25 2 26 9 16 0.055 509 Rowden 60 19 79 75 58 <0.001 510 M-315 Not included because of emergence problems Don Keim, Delta and Pine Land Co., P.O. Box 157, Scott, MS 38772 601 DLK-1 3 4 0 0 2 0.831 602 DLK-2 44 14 8 9 19 0.024 603 DLK-3 6 0 0 0 2 0.838 604 DLK-4 25 17 13 6 15 0.061 605 DLK-5 28 6 1 29 16 0.049 606 DLK-6 6 2 15 2 6 0.429 607 DLK-7 62 17 6 1 21 0.011 608 DLK-8 30 74 12 5 30 0.001 609 Rowden 80 54 71 61 66 <0.001 Not included because of emergence problems 610 M-315 continued 2004 NATIONAL COTTON FUSARIUM WILT REPORT 7 2005 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. P-value Richard Sheetz, Delta and Pine Land Co., RR 2, Box 60, Hale Center, TX 79041 701 RHS-1 11 5 7 23 12 0.008 702 RHS-2 5 5 6 7 6 0.183 703 RHS-3 2 18 0 3 6 0.145 704 RHS-4 1 2 1 0 1 0.788 705 RHS-6 9 6 4 9 7 0.096 706 RHS-7 1 1 11 1 4 0.388 707 RHS-8 3 9 1 1 4 0.366 708 RHS-8 3 10 0 6 5 0.231 709 Rowden 81 36 64 41 56 <0.001 Not included because of emergence problems 710 M-315 Curtis Williams, Delta and Pine Land Co., 381 William Gibbs Rd, Tifton, GA 31794 801 CW-1 32 12 41 30 29 0.001 802 CW-2 1 29 7 1 10 0.212 803 CW-3 1 31 22 11 16 0.043 804 CW-4 27 1 4 15 12 0.129 805 CW-5 4 8 5 5 5 0.498 806 CW-6 7 3 5 24 10 0.216 807 CW-7 4 0 13 12 7 0.358 808 CW-8 5 13 27 15 15 0.060 809 Rowden 34 24 56 72 47 <0.001 Not included because of emergence problems 810 M-315 Steve Calhoun, Emergent Genetics, 7624 Moore Road, Memphis, TN 38120 901 SC-1 8 4 2 0 902 SC-2 7 8 6 2 903 SC-3 4 0 0 0 904 SC-4 8 6 8 3 905 SC-5 11 13 13 30 906 SC-6 1 7 1 1 4 6 1 6 17 3 0.394 0.193 0.825 0.164 0.000 0.514 Al Balducchi, Emergent Genetics, 7624 Moore Road, Memphis, TN 38120 907 AB-1 2 0 0 0 1 0.902 908 AB-2 1 3 0 0 1 0.813 909 Rowden 63 57 53 49 56 <0.001 Not included because of emergence problems 910 M-315 continued 8 ALABAMA AGRICULTURAL EXPERIMENT STATION 2005 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. P-value Jack E. Jones, Jajo Genetics, 246 Maxine Dr., Baton Rouge, LA 70808-6831 1001 Jajo-1 18 19 6 7 13 0.229 1002 Jajo-2 5 1 8 10 6 0.556 1003 Jajo-3 38 8 20 34 25 0.023 1004 Jajo-4 40 10 1 2 13 0.205 1005 Jajo-5 6 27 16 3 13 0.223 1006 Jajo-6 6 0 8 100 29 0.010 1007 Jajo-7 21 8 9 7 11 0.283 1008 Jajo-8 26 9 20 11 16 0.124 1009 Rowden 72 33 83 85 68 <0.001 Not included because of emergence problems 1010 M-315 Dawn Fraser, Delta and Pine Land Co., P.O. Box 1529, Hartsville, SC 29550 1101 DF-1 7 4 23 1 1102 DF-2 11 8 4 11 1103 DF-3 4 10 3 0 1104 DF-4 10 10 10 4 1105 DF-5 26 11 28 3 1106 DF-6 20 5 11 9 1107 DF-7 1 5 9 9 9 9 4 9 17 11 6 0.140 0.136 0.484 0.140 0.006 0.058 0.285 Al Balducchi, Emergent Genetics, 7624 Moore Road, Memphis, TN 38120 1108 AB-6 1 4 3 4 3 0.557 1109 Rowden 48 42 69 81 60 <0.001 Not included because of emergence problems 1110 M-315 Larry Burdett, Delta and Pine Land Co., 38768 W. Farrell Rd, Maricopa, AZ 85239 1201 LB-1 2 6 0 6 4 0.500 1202 LB-2 34 3 4 3 11 0.048 1203 LB-3 1 2 3 0 2 0.779 1204 LB-4 1 6 7 5 5 0.364 1205 LB-5 5 4 2 7 4 0.416 1206 LB-6 9 21 6 0 9 0.104 1207 LB-7 10 17 5 9 10 0.061 1208 LB-8 17 8 4 11 10 0.075 1209 Rowden 26 46 85 27 46 <0.001 Not included because of emergence problems 1210 M-315 continued 2004 NATIONAL COTTON FUSARIUM WILT REPORT 9 2005 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. P-value Chris Tinius, Emergent Genetics, 7622 Moore Road, Memphis, TN 38120 1301 CT-1 4 16 2 0 6 0.249 1302 CT-2 16 32 21 5 19 0.001 1303 CT-3 1 3 3 0 2 0.697 1304 CT-4 7 5 6 3 5 0.266 1305 CT-5 11 15 11 5 11 0.033 1306 CT-6 1 4 0 0 1 0.808 1307 AB-3 6 14 4 0 6 0.209 1308 AB-4 0 2 4 0 1 0.783 1309 Rowden 53 27 40 56 44 <0.001 Not included because of emergence problems 1310 M-315 Mark Barfield, Emergent Genetics, 7622 Moore Road, Memphis, TN 38120 1401 MB1 9 9 18 5 1402 MB2 37 19 47 11 1403 MB3 12 9 7 10 1404 MB4 29 3 13 3 1405 MB5 3 3 2 0 1406 MB6 2 16 0 6 10 28 10 12 2 6 0.057 <0.001 0.073 0.033 0.703 0.252 Robert Cossar, Emergent Genetics, 7622 Moore Road, Memphis, TN 38120 1407 RC-1 3 5 3 1 3 0.555 1408 RC-2 21 37 22 7 22 0.000 1409 Rowden 29 71 70 47 54 <0.001 Not included because of emergence problems 1410 M-315 Jody Butler, Emergent Genetics, 7622 Moore Road, Memphis, TN 38120 1501 JB-1 19 22 1 4 1502 JB-2 4 7 0 3 1503 JB-3 20 4 27 1 1504 JB-4 8 7 13 13 1505 JB-5 11 14 10 5 1506 JB-6 0 12 4 5 11 4 13 10 10 5 0.053 0.535 0.033 0.091 0.098 0.370 Robert Cossar, Emergent Genetics, 7622 Moore Road, Memphis, TN 38120 1507 RC-3 1 4 0 12 4 0.452 1508 RC-4 6 0 16 12 8 0.153 1509 Rowden 51 40 71 32 48 <0.001 Not included because of emergence problems 1510 M-315 continued 10 ALABAMA AGRICULTURAL EXPERIMENT STATION 2005 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 21 15 18 9 1 1 Avg. 14 16 15 5 5 3 P-value 0.005 0.002 0.003 0.264 0.289 0.561 Randy Wood, Emergent Genetics, 7622 Moore Road, Memphis, TN 38120 1601 RW-1 7 8 21 1602 RW-2 11 17 21 1603 RW-3 18 19 4 1604 RW-4 1 7 4 1605 RW-5 0 14 4 1606 RW-6 3 0 7 Robert Cossar, Emergent Genetics, 7622 Moore Road, Memphis, TN 38120 1607 RC-5 3 1 5 0 2 0.614 1608 RC-6 5 4 6 3 5 0.324 1609 Rowden 46 29 86 69 57 <0.001 Not included because of emergence problems 1610 M-315 Peggy Thaxton, Texas A&M University , College Station, TX 77843-2474 1701 CIL-1 15 10 9 25 15 0.103 1702 CIL-2 21 29 69 14 33 0.001 1703 CIL-3 38 36 22 29 32 0.001 1704 CIL-4 16 16 3 8 10 0.247 1705 CIL-5 67 31 71 62 58 <0.001 1706 CIL-6 51 21 16 65 38 0.000 1707 CIL-7 41 3 22 12 19 0.036 1708 CIL-8 17 33 60 1 28 0.004 1709 Rowden 59 28 72 68 57 <0.001 Not included because of emergence problems 1710 M-315 Mike Robinson, Emergent Genetics, 7622 Moore Road, Memphis, TN 38120 1801 MR-1 18 43 4 4 1802 MR-2 6 10 3 4 1803 MR-3 12 10 14 3 1804 MR-4 11 0 3 0 1805 MR-5 8 4 6 12 1806 MR-6 4 0 1 0 17 6 10 3 8 1 0.001 0.252 0.049 0.466 0.115 0.798 Randell Lee, Emergent Genetics, 7622 Moore Road, Memphis, TN 38120 1807 RL-1 2 5 1 0 2 0.667 1808 RL-2 9 22 7 1 10 0.044 1809 Rowden 44 77 68 37 57 <0.001 Not included because of emergence problems 1810 M-315 continued 2004 NATIONAL COTTON FUSARIUM WILT REPORT 11 2005 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. P-value Charlie Cook, Syngenta Seeds, Inc., 356 Hosek Rd., Victoria, TX 77905-5636 1901 CC1 2 1 4 6 3 0.371 1902 CC2 3 11 5 4 6 0.132 1903 CC3 6 9 6 5 6 0.091 1904 CC4 11 35 5 13 16 <0.001 1905 CC5 6 9 2 6 6 0.136 1906 CC6 20 8 8 22 15 0.000 1907 CC7 16 16 7 5 11 0.006 1908 CC8 8 21 10 3 11 0.007 1909 Rowden 35 58 48 65 51 <0.001 Not included because of emergence problems 1910 M-315 Bon Prince, Syngenta Seeds, Inc., 356 Hosek Rd., Victoria, TX 77905-5636 2001 BP1 12 19 15 13 15 0.047 2002 BP2 62 7 4 8 20 0.008 2003 BP3 41 30 13 18 25 0.001 2004 BP4 18 35 9 5 17 0.026 2005 BP5 30 9 6 7 13 0.074 2006 BP6 8 4 2 10 6 0.405 2007 BP7 37 12 13 23 21 0.005 2008 BP8 28 25 3 2 15 0.045 2009 Rowden 72 65 48 38 56 <0.001 2010 M-315 Not included because of emergence problems Fred Bourland, University of Arkansas, P.O. Box 48, Keiser, AR 72351 2101 FB-1 5 10 7 13 9 0.098 2102 FB-2 13 6 8 9 9 0.101 2103 FB-3 6 5 4 10 6 0.243 2104 FB-4 1 2 58 2 16 0.005 2105 FB-5 2 12 1 3 5 0.387 2106 FB-6 0 1 6 0 2 0.719 2107 FB-7 4 2 2 4 3 0.556 2108 FB-8 3 22 14 4 11 0.041 2109 Rowden 66 53 55 54 57 <0.001 Not included because of emergence problems 2110 M-315 continued 12 ALABAMA AGRICULTURAL EXPERIMENT STATION 2005 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. P-value O. Lloyd May, Delta and Pine Land Co., 381 William Gibbs Rd, Tifton, GA 31794 2201 WL-1 39 11 22 30 26 0.007 2202 WL-2 9 56 5 12 20 0.027 2203 WL-3 52 16 73 40 45 <0.001 2204 WL-4 42 1 6 1 13 0.160 2205 WL-5 1 3 2 3 2 0.788 2206 WL-6 25 12 4 15 14 0.123 2207 WL-7 58 12 10 5 21 0.022 2208 WL-8 39 52 30 14 34 0.001 2209 Rowden 64 88 62 65 70 <0.001 Not included because of emergence problems 2210 M-315 Joe Ware, Emergent Genetics, 7622 Moore Road, Memphis, TN 38120 2301 JW-1 42 33 49 2302 JW-2 3 3 2 2303 JW-3 13 8 3 2304 JW-4 22 34 39 2305 JW-5 8 6 9 2306 JW-6 8 12 8 Randell Lee, Emergent Genetics, 7622 Moore Road, Memphis, TN 38120 2307 RL-3 5 16 14 28 1 9 30 6 5 6 38 2 8 31 7 8 10 <0.001 0.660 0.108 <0.001 0.189 0.122 0.056 Kathryn Glass, Agronomy & Soils Dept., 201 Funchess Hall, Auburn University, AL 36849 2308 PHY 470 WR 2 11 8 9 7 0.157 2309 Rowden 61 52 54 27 49 <0.001 Not included because of emergence problems 2310 M-315 Jeff Klingenberg, Bayer Crop Science, 1602 Paradise Dr., Sellers, SC 25992 2401 BCSI-JK-1 7 20 3 8 10 0.007 2402 BCSI-JK-2 20 6 5 15 12 0.002 2403 BCSI-JK-3 30 18 4 26 19 <0.001 2404 BCSI-JK-4 1 16 7 17 10 0.005 2405 BCSI-JK-5 22 4 4 11 10 0.005 2406 BCSI-JK-6 13 5 4 1 6 0.103 2407 BCSI-JK-7 11 5 1 3 5 0.143 2408 BCSI-JK-8 5 5 4 4 4 0.191 2409 Rowden 63 64 66 73 67 <0.001 Not included because of emergence problems 2410 M-315 continued 2004 NATIONAL COTTON FUSARIUM WILT REPORT 13 2005 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. P-value Steve Hague, Bayer Crop Science, 117 Kennedy Flat Rd., Leland, MS 38756 2501 BCSI-SH-1 3 1 9 1 4 0.212 2502 BCSI-SH-2 23 14 18 3 14 <0.001 2503 BCSI-SH-3 14 5 19 16 14 <0.001 2504 BCSI-SH-4 7 21 10 18 14 <0.001 2505 BCSI-SH-5 14 4 6 3 7 0.025 2506 BCSI-SH-6 11 21 15 18 17 <0.001 2507 BCSI-SH-7 12 10 14 13 12 0.000 2508 BCSI-SH-8 0 11 4 2 4 0.132 2509 Rowden 55 64 70 65 64 <0.001 Not included because of emergence problems 2510 M-315 Michael Swindle, Bayer Crop Science, 117 Kennedy Flat Rd., Leland, MS 38756 2601 BCSI-MS-1 28 22 26 14 23 0.001 2602 BCSI-MS-2 13 15 7 4 10 0.116 2603 BCSI-MS-3 26 29 20 9 21 0.002 2604 BCSI-MS-4 4 2 9 2 4 0.481 2605 BCSI-MS-5 13 20 26 1 15 0.018 2606 BCSI-MS-6 3 18 5 6 8 0.188 2607 BCSI-MS-7 9 8 11 5 8 0.185 2608 BCSI-MS-8 52 4 11 2 17 0.008 2609 Rowden 68 80 46 33 57 <0.001 Not included because of emergence problems 2610 M-315 Bruce Kirksey, Emergent Genetics, 7622 Moore Road, Memphis, TN 38120 2701 BK-1 3 13 12 4 2702 BK-2 4 1 0 0 2703 BK-3 10 3 4 8 2704 BK-4 6 11 16 1 2705 BK-5 11 4 26 8 2706 BK-6 24 3 8 1 8 1 6 8 12 9 0.053 0.750 0.120 0.041 0.004 0.030 Kathryn Glass, Agronomy & Soils Dept., 201 Funchess Hall, Auburn University, AL 36849 2707 ST 5599BR 18 0 2 6 7 0.102 2708 FM 960BR 1 4 9 1 4 0.343 2709 Rowden 59 24 49 51 46 <0.001 Not included because of emergence problems 2710 M-315 continued 14 ALABAMA AGRICULTURAL EXPERIMENT STATION 2005 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 4 6 5 8 1 3 Avg. 16 19 9 24 1 9 P-value 0.020 0.007 0.178 0.001 0.928 0.177 Brent Styles, Emergent Genetics, 7622 Moore Road, Memphis, TN 38120 2801 BS-1 33 27 2 2802 BS-2 50 18 3 2803 BS-3 8 2 21 2804 BS-4 42 13 34 2805 BS-5 0 0 1 2806 BS-6 4 22 8 Kathryn Glass, Agronomy & Soils Dept., 201 Funchess Hall, Auburn University, AL 36849 2807 DP 424BGII/RR 2 1 4 8 4 0.582 2808 DP 555BG/RR 45 7 8 12 18 0.012 2809 Rowden 64 80 73 42 65 <0.001 Not included because of emergence problems 2810 M-315