2004 National Cotton Fusarium Wilt Report September 2004 AGRONOMY AND SOILS DEPARTMENT SERIES NO. 262 ALABAMA AGRICULTURAL EXPERIMENT STATION MICHAEL J. WEISS, DIRECTOR AUBURN UNIVERSITY AUBURN, AL 36849 THIS REPORT IS A JOINT CONTRIBUTION BETWEEN USDA-ARS, CROP SCIENCE RESEARCH LABORATORY, MISSISSIPPI STATE UNIVERSITY, MISSISSIPPI, AND THE ALABAMA AGRICULTURAL EXPERIMENT STATION, AUBURN UNIVERSITY, ALABAMA Information contained herein is available to all persons regardless of race, color, sex, or national origin 2004 NATIONAL COTTON FUSARIUM WILT REPORT 3 2004 NATIONAL COTTON FUSARIUM WILT REPORT Kathryn M. Glass1, William S. Gazaway2, and Edzard van Santen 3 1,3 Agricultural Program Associate and Professor, respectively, Dept. of Agronomy and Soils, Auburn University, AL 36849 2 Professor Emeritus and Extension Pathologist/Nematologist, Dept. of Entomology and Plant Pathology, Auburn University, 36849 Cotton cultivars and elite breeding lines submitted by 30 cooperators were evaluated for Fusarium wilt resistance under field conditions at the E. V. Smith Research Center, Plant Breeding Unit, Tallassee, Alabama. These entries were grown on an Independence loamy fine sand highly infested with the Fusarium wilt fungus (Fusarium oxysporum) Schlect. f. vasinfectum [Atk.] (Snyd. & Hans.) and southern root-knot nematodes (Meloidogyne incognita.). In 1994, a soil analysis for nematodes revealed that southern root-knot (Meloidogyne incognita) and lance (Hoplolaimus galeatus) are the predominant nematode species in the test plots. High populations of both species are found throughout the test area. Other nematode genera present are stubby root (Trichodorus sp.) and stunt (Tylenchorhynchus sp.). Root-knot nematodes, however, appear to be causing the major damage to cotton in the Fusarium Wilt Test as indicated by the high galling indices found on the roots of all cotton lines. Entries were planted in single 20-foot rows on 40-inch centers, separated by 5-foot alleys. Four replications of the test entries and checks were evaluated in a randomized complete block design with a split plot restriction on randomization. The set of eight test cultivars submitted by a cooperator was always evaluated as a group together with two control plots within each replicate. Both susceptible (Rowden) and resistant (M-315) cultivars were included as check subplots in the two center rows of each main plot (Fig. 1). Plots were planted May 20. Initial plant counts were made on June 17. Wilted plants were counted and removed on July 1, July 15, July 29, August 10, and August 26. The remaining live plants were counted and recorded on August 26. Total percent wilted plants were then determined and mean wilting for a given entry calculated. The average % wilted plants for the susceptible check Rowden was 65%, with a range from 0 to 100% on an individual plot basis (Fig. 1). Given the large variability of wilt development in Rep 4 ( minimum 0%, average 29%, maximum 93%) resulting in a range of 93% compared to 60-70% for the other replicates, it might be advisable for individual cooperators ignore data from Rep 4. The resistant check M-315 had, on the average, 3 % wilted plants with a range from 0 to 21% on an individual plot basis. There were, on the average, 3x more wilted plants in Rowden plots than in M315 plots if all replicates are considered and 25x for replicates 1-3. Critical evaluations of breeding lines should be made relative to the two checks listed at the bottom of each group. It was a very wet spring. Sulfur defiency was noted in early season and corrected through foliar application of Magnesium sulfate at 2 lbs/acre. 4 ALABAMA AGRICULTURAL EXPERIMENT STATION Fig. 1. Field plot layout and % wilt for control plot of Rowden (suceptible) and M-315 (resistant). Distances (ft) from the NE corner of the trial are given in the left hand column and the bottom row. NS 500 475 450 425 400 375 350 325 300 275 93 0 Rowden M-315 74 2 Rowden M-315 82 0 Rowden M-315 0 M-315 6 M-315 56 Rowden 82 Rowden 0 M-315 14 Rowden 0 M-315 26 Rowden 0 M-315 34 Rowden 0 M-315 83 Rowden 0 M-315 1 M-315 37 Rowden 0 M-315 14 Rowden 1 M-315 46 Rowden 18 M-315 81 Rowden 0 M-315 94 Rowden 66 Rowden 5 M-315 0 M-315 0 M-315 0 M-315 1 M-315 0 M-315 80 Rowden 1 M-315 64 Rowden 4 M-315 38 Rowden 2 Rowden 5 Rowden 13 Rowden 11 Rowden 89 Rowden 0 M-315 73 Rowden 0 M-315 82 Rowden 0 M-315 8 Rowden 0 M-315 0 Rowden 2 M-315 2 M-315 0 M-315 81 Rowden 73 Rowden 96 Rowden 25 Rowden 0 M-315 2 Rowden 0 M-315 3 Rowden 21 Rowden 89 Rowden 1 M-315 3 M-315 2 M-315 0 M-315 1 M-315 19 Rowden 37 Rowden 5 M-315 1 M-315 1 M-315 4 M-315 37 Rowden 2 Rowden 2 Rowden 0 M-315 0 M-315 41 Rowden 58 Rowden 75 Rowden 58 Rowden 3 M-315 100 6 Rowden M-315 90 1 Rowden M-315 100 4 Rowden M-315 4 M-315 1 M-315 98 Rowden 92 Rowden 8 rows 8 rows 8 rows 8 rows 8 rows 250 225 200 175 150 125 100 75 50 25 0 M-315 89 Rowden 3 M-315 63 Rowden 6 M-315 76 Rowden 0 M-315 63 Rowden 62 Rowden 3 M-315 3 M-315 67 Rowden 72 Rowden 3 M-315 71 Rowden 0 M-315 63 Rowden 3 M-315 0 M-315 68 Rowden 85 Rowden 0 M-315 1 M-315 78 Rowden 1 M-315 40 Rowden 0 M-315 1 M-315 2 M-315 1 M-315 1 M-315 64 Rowden 43 Rowden 0 M-315 95 Rowden 2 M-315 85 Rowden 73 Rowden 40 Rowden 70 Rowden 67 Rowden 4 M-315 3 M-315 89 Rowden 4 M-315 0 M-315 1 M-315 0 M-315 4 M-315 79 Rowden 0 M-315 38 Rowden 61 Rowden 0 M-315 80 Rowden 76 Rowden 77 Rowden 97 Rowden 99 Rowden 11 M-315 54 Rowden 3 M-315 79 Rowden 58 Rowden 72 Rowden 79 Rowden 84 Rowden 0 M-315 88 Rowden 0 M-315 1 M-315 55 Rowden 2 M-315 3 M-315 2 M-315 6 M-315 4 M-315 92 Rowden 11 M-315 88 Rowden 86 Rowden 0 M-315 79 17 Rowden M-315 100 21 Rowden M-315 4 M-315 100 Rowden N 100 5 Rowden M-315 7 M-315 98 Rowden 100 12 Rowden M-315 4 M-315 4 M-315 100 Rowden 91 Rowden 0 EW ==> 66 3 Rowden M-315 27 60 93 127 160 8 rows 0 M-315 89 Rowden 85 Rowden 1 M-315 57 Rowden 2 M-315 7 M-315 86 Rowden 70 Rowden 8 M-315 2004 NATIONAL COTTON FUSARIUM WILT REPORT 5 2004 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Entry Cultivar/Line 101 FB-1 102 FB-2 103 FB-3 104 FB-4 105 FB-5 106 FB-6 107 FB-7 108 FB-8 Rowden M-315 201 DKL-103 202 DKL-203 203 DKL-303 204 DKL-403 205 DKL-503 206 DKL-603 207 DKL-703 208 DKL-804 Rowden M-315 301 PHY-FB1 302 PHY-FB2 303 PHY-FB3 304 PHY-FB4 305 PHY-FB5 306 PHY-FB6 307 PHY-FB7 308 PHY-FB8 Rowden M-315 Rep1 64 81 36 56 22 13 62 66 100 12 31 64 14 25 45 78 20 40 73 1 43 40 18 4 20 10 17 9 91 4 Percent wilted plants Rep2 Rep3 Rep4 95 82 2 5 29 5 35 18 84 4 6 25 16 21 21 3 10 0 63 3 1 2 16 8 12 0 10 4 76 0 76 60 16 36 59 7 44 75 92 1 51 48 19 78 5 83 67 9 82 4 18 33 6 14 5 10 0 18 80 0 35 5 1 3 30 0 3 1 46 0 2 2 3 1 0 10 0 0 37 0 0 0 0 1 0 0 1 4 3 2 Avg. 67 57 14 25 35 6 36 40 80 4 23 35 13 31 18 44 24 12 64 2 16 19 10 7 9 5 7 8 62 1 P-value <.0001 <.0001 0.146 0.010 0.001 0.510 0.000 <.0001 <.0001 0.656 0.019 0.001 0.178 0.002 0.061 <.0001 0.013 0.200 <.0001 0.842 0.101 0.052 0.288 0.477 0.320 0.606 0.442 0.372 <.0001 0.882 continued THE P-VALUE INDICATES THE PROBABILITY THAT THE AVERAGE WILT PERCENTAGE DIFFERS FROM ZERO. Fred Bourland,University of Arkansas, P.O. Box 48, Keiser, AR 72351 Don Keim,Delta and Pine Land Co., P.O. Box 157, Scott, MS 38772 Frank Bordelon,PhytoGen Seed Company, P.O. Box 27, Leland, MS 38756 6 ALABAMA AGRICULTURAL EXPERIMENT STATION 2004 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Entry Cultivar/Line 401 PHY-RM1 402 PHY-RM2 403 PHY-RM3 404 PHY-RM4 405 PHY-RM5 406 PHY-RM6 407 PHY-RM7 408 PHY-RM8 Rowden M-315 501 GLR-1 502 GLR-2 503 GLR-3 504 GLR-4 505 GLR-5 506 GLR-6 507 GLR-7 508 GLR-8 Rowden M-315 601 JNK-1 602 JNK-2 603 JNK-3 604 JNK-4 605 JNK-5 606 JNK-6 607 JNK-7 608 JNK-8 Rowden M-315 Rep1 8 0 3 9 10 2 13 2 63 3 37 7 6 31 53 60 57 8 98 7 11 17 6 18 12 8 5 13 99 4 Percent wilted plants Rep2 Rep3 Rep4 7 9 3 3 15 26 17 3 85 0 27 0 0 16 35 6 57 0 43 1 18 8 4 44 22 19 0 11 63 0 4 16 0 0 1 2 2 3 81 0 0 3 3 7 24 19 41 0 89 0 0 19 20 6 6 5 0 6 75 1 0 1 4 11 4 4 3 1 74 2 0 0 2 2 3 0 16 2 2 0 1 0 0 1 4 0 0 0 19 0 Avg. 5 7 2 6 8 9 9 2 76 1 16 3 3 14 29 21 43 2 58 2 8 11 8 17 11 8 1 8 64 1 P-value 0.625 0.491 0.809 0.527 0.427 0.364 0.345 0.817 <.0001 0.897 0.096 0.778 0.790 0.144 0.004 0.028 <.0001 0.804 <.0001 0.832 0.418 0.252 0.427 0.071 0.252 0.396 0.895 0.427 <.0001 0.893 continued Mustafa McPherson,PhytoGen Seed Company, P.O. Box 27, Leland, MS 38756 Gary L. Rea,Delta and Pine Land Co., 247 US HWY 380 W, Haskell, TX 79521 Johnie Jenkins,USDA-ARS, Mississippi State, MS 39762 2004 NATIONAL COTTON FUSARIUM WILT REPORT 7 2004 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. 14 12 11 7 10 5 7 13 56 1 P-value 0.154 0.210 0.246 0.056 0.276 0.629 0.471 0.174 <.0001 0.950 0.000 <.0001 <.0001 0.019 0.001 <.0001 0.007 0.002 <.0001 0.716 Richard Sheetz,Delta and Pine Land Co., RR 2, Box 60, Hale Center, TX 79041 701 RHS-1 23 18 11 3 702 RHS-2 703 RHS-3 704 RHS-4 705 RHS-6 706 RHS-7 707 RHS-8 708 RHS-8 Rowden M-315 28 0 52 9 12 16 13 40 2 17 21 5 21 5 8 28 78 0 0 22 6 10 0 3 9 83 0 2 1 3 2 2 0 1 25 0 Curtis Williams,Delta and Pine Land Co., 381 William Gibbs Rd, Tifton, GA 31794 801 CW-1 77 27 36 6 36 802 CW-2 46 64 87 40 59 803 CW-3 804 CW-4 805 CW-5 806 CW-6 807 CW-7 808 CW-8 Rowden M-315 75 58 89 100 66 60 100 5 26 5 14 57 24 26 72 2 48 27 33 95 8 29 57 2 14 0 2 17 8 7 41 5 41 23 35 67 27 30 67 3 Steve Calhoun,Stoneville Pedigreed Seed Co., Rt. 2, Box 233, Hwy 82 East, Idalou, TX 79239 901 SC-1 10 10 5 0 6 0.512 902 SC-2 6 1 5 0 3 0.738 903 SC-3 8 25 0 7 10 0.293 904 SC-4 36 35 4 17 23 0.019 Jody Butler,Stoneville Pedigreed Seed Co., 2409 Commerce Lane, Albany, GA 31707 905 JB-1 9 2 38 4 13 906 JB-2 29 6 31 3 17 907 JB-3 27 29 14 0 18 Daryl Bowman,North Carolina State University, Raleigh, NC 908 NC 96-11 91 84 Rowden 79 85 M-315 11 1 83 94 0 83 13 0 85 68 3 0.162 0.071 0.067 <.0001 <.0001 0.755 continued 8 ALABAMA AGRICULTURAL EXPERIMENT STATION 2004 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Entry Cultivar/Line Rep1 4 0 5 6 27 2 3 24 55 0 0 38 13 18 2 4 7 0 68 3 14 11 0 18 2 30 3 10 67 1 Percent wilted plants Rep2 Rep3 Rep4 11 9 24 28 21 17 10 15 77 1 16 25 50 30 14 25 14 43 89 0 0 22 10 12 6 59 35 49 100 4 62 13 13 6 34 12 25 13 96 2 58 80 10 26 6 4 24 50 98 4 0 3 8 2 0 2 5 27 58 1 68 31 4 0 53 5 0 36 56 0 0 2 0 0 0 0 6 0 0 0 3 3 0 0 2 7 10 8 11 1 Avg. 36 13 11 10 34 9 10 22 71 1 19 36 18 18 6 8 13 23 64 2 4 10 4 8 3 24 13 23 59 2 P-value 0.000 0.159 0.234 0.290 0.001 0.344 0.305 0.023 <.0001 0.935 0.052 0.000 0.058 0.057 0.555 0.381 0.184 0.017 <.0001 0.860 0.651 0.294 0.649 0.415 0.783 0.012 0.162 0.016 <.0001 0.841 continued Jack E. Jones,Jajo Genetics, 246 Maxine Dr., Baton Rouge, LA 70808-6831 1001 Jajo-1 1002 Jajo-2 1003 Jajo-3 1004 Jajo-4 1005 Jajo-5 1006 Jajo-6 1007 Jajo-7 1008 Jajo-8 Rowden M-315 1101 DF-1 1102 DF-2 1103 DF-3 1104 DF-4 1105 DF-5 1106 DF-6 1107 DF-7 1108 DF-8 Rowden M-315 1201 DW-1 1202 DW-2 1203 DW-3 1204 DW-4 1205 DW-5 1206 DW-6 1207 DW-7 1208 DW-8 Rowden M-315 Dawn Fraser,Delta and Pine Land Co., P.O. Box 1529, Hartsville, SC 29550 Doug Wessel,Delta and Pine Land Co., 38768 W. Farrell Rd, Maricopa, AZ 85239 2004 NATIONAL COTTON FUSARIUM WILT REPORT 9 2004 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. P-value 0.095 0.373 0.317 0.443 0.331 0.003 0.002 0.227 <.0001 0.576 0.791 0.242 0.007 0.000 0.071 0.024 0.108 0.073 <.0001 0.774 continued Chris Tinius,Stoneville Pedigreed Seed Co., 7622 Moore Road, Memphis, TN 38120 1301 CT1 24 32 7 0 16 1302 CT2 18 14 1 1 8 1303 CT3 26 9 0 3 9 1304 CT4 14 9 4 2 7 1305 CT5 2 33 2 0 9 1306 CT6 48 46 22 1 29 Ira Stein,Stoneville Pedigreed Seed Co., 2409 Commerce Lane, Albany, GA 31707 1307 IS-1 51 42 20 10 31 1308 IS-2 17 21 3 5 11 Rowden 67 95 34 26 55 M-315 0 1 18 1 5 Mark Barfield,Stoneville Pedigreed Seed Co., 2409 Commerce Lane, Albany, GA 31707 1401 MB1 0 10 0 0 3 1402 MB2 11 20 14 0 11 1403 MB3 45 34 26 0 26 1404 MB4 45 40 55 9 37 1405 MB5 43 7 17 3 17 1406 MB6 28 11 48 0 22 Ira Stein,Stoneville Pedigreed Seed Co., 2409 Commerce Lane, Albany, GA 31707 1407 IS-3 51 8 0 2 15 1408 IS-4 57 4 8 0 17 Rowden 100 76 86 14 69 M-315 4 0 7 0 3 10 ALABAMA AGRICULTURAL EXPERIMENT STATION 2004 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. P-value 0.002 0.463 0.428 0.559 0.409 0.000 0.453 <.0001 <.0001 0.538 0.105 0.030 0.025 0.027 0.027 0.479 0.031 0.423 <.0001 0.877 0.825 0.860 0.850 0.900 0.919 0.898 1.000 0.018 <.0001 0.907 continued Al Balducchi,Stoneville Pedigreed Seed Co., 7622 Moore Rd, Memphis, TN 38120 1501 AB1 29 70 19 3 30 1502 AB2 1503 AB3 1504 AB4 1505 AB5 1506 AB6 1507 IS-5 1508 IS-6 Rowden M-315 1601 RW1 1602 RW2 1603 RW3 1604 RW4 1605 RW5 1606 RW6 3 14 8 0 59 2 65 70 1 37 17 38 16 27 14 18 13 11 19 59 22 42 100 21 9 60 26 65 39 6 7 4 3 13 26 4 67 90 1 16 4 20 4 19 7 0 0 0 0 4 0 5 8 0 0 3 2 0 0 0 7 7 6 8 37 7 45 67 6 15 21 22 21 21 7 Ira Stein,Stoneville Pedigreed Seed Co., 2409 Commerce Lane, Albany, GA 31707 Randy Wood,Stoneville Pedigreed Seed Co., P.O. Box 569, Maricopa, AZ 85239 Joe Ware,Stoneville Pedigreed Seed Co., 2409 Commerce Lane, Albany, GA 31707 1607 JW-1 25 41 8 10 21 1608 JW-2 6 9 15 0 8 Rowden 88 40 58 5 48 M-315 0 2 4 0 1 Laval Verhalen,Oklahoma State University, 368 Agricultural Hall, Stillwater, OK 74078 1701 OKLA-1 8 0 0 0 2 1702 OKLA-2 0 0 0 7 2 1703 OKLA-3 0 7 0 0 2 1704 OKLA-4 0 5 0 0 1 1705 OKLA-5 0 4 0 0 1 1706 OKLA-6 0 5 0 0 1 1707 OKLA-7 0 0 0 0 0 1708 OKLA-8 13 35 39 6 23 Rowden 86 58 89 14 62 M-315 1 3 0 0 1 2004 NATIONAL COTTON FUSARIUM WILT REPORT 11 2004 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Entry Cultivar/Line Rep1 9 15 33 6 17 34 7 7 64 4 0 8 7 9 65 2 14 16 38 3 Percent wilted plants Rep2 Rep3 Rep4 18 7 63 11 18 45 3 43 71 6 6 2 6 21 68 73 37 25 79 6 18 1 79 22 7 18 31 23 73 3 0 5 22 34 84 22 29 16 37 3 0 0 20 0 1 1 0 15 37 0 1 17 44 9 72 11 19 62 82 6 Avg. 11 6 49 10 11 25 10 22 61 3 2 8 20 18 72 27 25 30 59 4 P-value 0.230 0.546 <.0001 0.293 0.253 0.012 0.269 0.022 <.0001 0.732 0.859 0.414 0.038 0.058 <.0001 0.006 0.011 0.002 <.0001 0.647 continued Mike Robinson,Stoneville Pedigreed Seed Co., 4852 Old Leland Rd, Leland, MS 38776 1801 MR1 1802 MR2 1803 MR3 1804 MR4 1805 MR5 1806 MR6 1807 JW-3 1808 JW-4 Rowden M-315 1901 CC1 1902 CC2 1903 CC3 1904 CC4 1905 CC5 1906 CC6 1907 CC7 1908 CC8 Rowden M-315 Joe Ware, Bayer Crop Science, 1602 Paradise Dr., Sellers, SC 25992 Charlie Cook,Syngenta Seeds, Inc., 356 Hosek Rd., Victoria, TX 77905-5636 12 ALABAMA AGRICULTURAL EXPERIMENT STATION 2004 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Entry Cultivar/Line Rep1 Percent wilted plants Rep2 Rep3 Rep4 Avg. 83 8 17 59 60 28 P-value <.0001 0.382 0.073 <.0001 <.0001 0.005 0.003 Bon Prince,Syngenta Seeds, Inc., 356 Hosek Rd., Victoria, TX 77905-5636 2001 BP1 100 96 96 43 2002 BP2 19 10 1 4 2003 BP3 12 47 7 2 2004 BP4 60 42 100 35 2005 BP5 30 97 88 27 2006 BP6 5 95 8 4 Joe Ware,Stoneville Pedigreed Seed Co., 2410 Commerce Lane, Albany, GA 31707 2007 JW-5 20 63 32 4 30 Kathryn Glass,Agronomy & Soils Dept., 201 Funchess Hall, Auburn University, AL 36849 2008 DPL 424 BGII/RR 0 0 0 0 0 1.000 Rowden 97 79 100 21 74 <.0001 M-315 0 17 6 2 6 0.515 Jack McCarty,USDA, Mississippi State, MS 39762 2101 JCM-1 3 2102 JCM-2 24 2103 JCM-3 4 2104 JCM-4 21 2105 JCM-5 32 2106 JCM-6 6 2107 JCM-7 20 2108 JCM-8 21 Rowden 66 M-315 3 9 14 16 13 28 14 13 11 79 2 18 38 24 3 31 23 8 3 73 1 0 0 4 0 0 0 0 0 2 1 7 19 12 9 23 11 10 9 55 2 0.431 0.046 0.205 0.325 0.019 0.260 0.286 0.357 <.0001 0.847 continued 2004 NATIONAL COTTON FUSARIUM WILT REPORT 13 2004 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Entry Cultivar/Line Rep1 56 25 44 27 33 50 19 35 85 3 27 10 36 9 51 24 39 9 62 0 37 54 48 10 5 7 4 3 88 11 Percent wilted plants Rep2 Rep3 Rep4 6 33 33 0 27 7 18 21 72 3 27 5 19 18 23 10 22 6 61 3 26 35 30 21 7 13 7 4 80 4 26 17 28 35 8 0 12 6 70 8 0 21 13 2 37 1 63 14 66 1 56 21 90 12 72 62 56 1 100 4 3 9 1 23 8 18 14 36 82 0 6 1 3 0 0 0 0 4 2 0 30 16 20 3 21 3 7 0 93 0 Avg. 23 21 27 21 19 19 16 24 77 4 15 9 18 7 28 9 31 8 47 1 37 31 47 12 26 21 18 2 90 5 P-value 0.020 0.029 0.006 0.028 0.047 0.051 0.101 0.012 <.0001 0.707 0.114 0.325 0.064 0.446 0.005 0.357 0.002 0.377 <.0001 0.902 0.000 0.002 <.0001 0.221 0.007 0.028 0.055 0.821 <.0001 0.599 continued O. Lloyd May,University of Georgia, Coastal Plain Exp. Station, Tifton, GA 31793 2201 GA-200035 2202 GA-200036 2203 GA-200042 2204 GA-2001078 2205 GA-2002167 2206 GA-2002207 2207 GA-2002212 2208 GA-99090 Rowden M-315 2301 RGC-1 2302 RGC-2 2303 RGC-3 2304 RGC-4 2305 RGC-5 2306 RGC-6 2307 RGC-7 2308 RGC-8 Rowden M-315 2401 BCSI-JK-1 2402 BCSI-JK-2 2403 BCSI-JK-3 2404 BCSI-JK-4 2405 BCSI-JK-5 2406 BCSI-JK-6 2407 BCSI-JK-7 2408 BCSI-JK-8 Rowden M-315 Roy Creech,USDA, Mississippi State, MS 39762 Jeff Klingenberg,Bayer Crop Science, 1602 Paradise Dr., Sellers, SC 25992 14 ALABAMA AGRICULTURAL EXPERIMENT STATION 2004 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Entry Cultivar/Line Rep1 30 16 83 23 5 29 15 2 54 0 31 53 20 8 1 64 12 33 92 0 Percent wilted plants Rep2 Rep3 Rep4 22 8 35 26 10 27 10 2 89 0 55 69 32 9 59 57 33 29 89 0 8 5 32 13 14 56 11 0 81 1 28 16 22 2 37 90 2 48 64 0 0 0 0 0 0 1 0 2 2 0 10 31 0 3 5 7 12 5 38 5 Avg. 15 7 37 15 7 28 9 1 57 0 31 43 19 6 26 54 14 29 71 1 P-value 0.117 0.440 0.000 0.108 0.442 0.004 0.350 0.894 <.0001 0.973 0.002 <.0001 0.053 0.553 0.009 <.0001 0.129 0.004 <.0001 0.885 Jeff Gwyn,Bayer Crop Science, 117 Kennedy Flat Rd., Leland, MS 38756 2501 BCSI-JJG-1 2502 BCSI-JJG-2 2503 BCSI-JJG-3 2504 BCSI-JJG-4 2505 BCSI-JJG-5 2506 BCSI-JJG-6 2507 BCSI-JJG-7 2508 BCSI-JJG-8 Rowden M-315 2601 BCSI-MS-1 2602 BCSI-MS-2 2603 BCSI-MS-3 2604 BCSI-MS-4 2605 BCSI-MS-5 2606 BCSI-MS-6 2607 BCSI-MS-7 2608 BCSI-MS-8 Rowden M-315 Michael Swindle,Bayer Crop Science, 124 Kennedy Flat Rd., Leland, MS 38756