2001 National Cotton Fusarium Wilt Report NOVEMBER 2001 AGRONOMY AND SOILS DEPARTMENT SERIES NO. 238 ALABAMA AGRICULTURAL EXPERIMENT STATION ROBIN HUETTEL, EXECUTIVE ASSOCIATE DIRECTOR AUBURN UNIVERSITY AUBURN, AL 36849 THIS REPORT IS A JOINT CONTRIBUTION BETWEEN USDA-ARS, CROP SCIENCE RESEARCH LABORATORY, MISSISSIPPI STATE UNIVERSITY, MISSISSIPPI, AND THE ALABAMA AGRICULTURAL EXPERIMENT STATION, AUBURN UNIVERSITY, ALABAMA Information contained herein is available to all persons regardless of race, color, sex, or national origin 2001 NATIONAL COTTON FUSARIUM WILT REPORT 3 2001 NATIONAL COTTON FUSARIUM WILT REPORT Kathryn M. Glass1, William S. Gazaway2, and Edzard van Santen 3 1,3 Agricultural Program Associate and Associate Professor, respectively, Dept. of Agronomy and Soils, Auburn University, AL 36849 2 Professor Emeritus and Extension Pathologist/Nematologist, Dept. of Entomology and Plant Pathology, Auburn University, 36849 Cotton cultivars and elite breeding lines submitted by 24 cooperators were evaluated for Fusarium wilt resistance under field conditions at the E. V. Smith Research Center, Plant Breeding Unit, Tallassee, Alabama. These entries were grown on an Independence loamy fine sand highly infested with the Fusarium wilt fungus (Fusarium oxysporum) Schlect. f. vasinfectum [Atk.] (Snyd. & Hans.) and southern root-knot nematodes (Meloidogyne incognita.). In 1994, a soil analysis for nematodes revealed that southern root-knot (Meloidogyne incognita) and lance (Hoplolaimus galeatus) are the predominant nematode species in the test plots. High populations of both species are found throughout the test area. Other nematode genera present are stubby root (Trichodorus sp.) and stunt (Tylenchorhynchus sp.). Root-knot nematodes, however, appear to be causing the major damage to cotton in the Fusarium Wilt Test as indicated by the high galling indices found on the roots of all cotton lines. Entries were planted in single 20-foot rows on 40-inch centers, separated by 5-foot alleys. Four replications of the test entries and checks were evaluated in a randomized complete block design with a split plot restriction on randomization. The set of eight test cultivars submitted by a cooperator was always evaluated as a group together with two control plots within each replicate. Both susceptible (Rowden) and resistant (M-315) cultivars were included as check subplots in the two center rows of each main plot (Fig. 1). Initial plant counts were made on June 26. Wilted plants were counted and removed on July 13, July 31, August 17, and September 5. The remaining live plants were counted and recorded on September 5. Total percent wilted plants were then determined and mean wilting for a given entry calculated. The average % wilted plants for the susceptible check Rowden was 66%, with a range from 4 to 100% on an individual plot basis (Fig. 1). The resistant check M-315 had, on the average, 6% wilted plants with a range from 0 to 39% on an individual plot basis. There were, on the average, 11x more wilted plants in Rowden plots than in M-315 plots. Critical evaluations of breeding lines should be made relative to the two checks listed at the bottom of each group. 4 ALABAMA AGRICULTURAL EXPERIMENT STATION Fig. 1. Field plot layout and % wilt for control plot of Rowden (suceptible) and M-315 (resistant). Distances (ft) from the SE corner of the trial are given in the left hand column and the bottom row. E-W 525 500 475 450 425 400 375 350 325 300 4 M-315 79 Rowden 5 M-315 86 Rowden 6 M-315 75 Rowden 1 M-315 77 Rowden 1 M-315 81 Rowden 7 M-315 75 Rowden 4 225 200 175 150 125 100 75 50 25 0 SE corner M-315 75 Rowden 7 M-315 89 Rowden 0 M-315 96 Rowden 18 M-315 69 Rowden 10 M-315 96 Rowden 20 43 Rowden 4 M-315 87 Rowden 0 M-315 76 Rowden 6 M-315 90 Rowden 0 M-315 97 Rowden 2 M-315 74 Rowden 2 M-315 69 Rowden 17 M-315 74 Rowden 3 M-315 62 Rowden 3 M-315 82 Rowden 4 M-315 79 Rowden 1 M-315 33 Rowden 0 M-315 36 Rowden 2 M-315 67 Rowden 0 M-315 79 Rowden 5 M-315 82 Rowden 6 M-315 82 Rowden 3 M-315 51 Rowden 39 M-315 35 Rowden 8 M-315 95 Rowden 4 M-315 89 Rowden 10 M-315 91 Rowden 12 M-315 53 4 M-315 100 Rowden 4 M-315 90 Rowden 9 M-315 95 Rowden 4 M-315 79 Rowden 2 M-315 94 Rowden 1 M-315 14 Rowden 7 M-315 46 Rowden 5 M-315 87 Rowden 9 M-315 85 Rowden 12 M-315 94 Rowden 24 M-315 91 Rowden 0 M-315 78 Rowden 1 M-315 48 Rowden 0 M-315 36 Rowden 3 M-315 26 Rowden 4 M-315 66 Rowden 0 M-315 19 Rowden 4 M-315 64 Rowden 19 M-315 45 Rowden 4 M-315 75 Rowden 3 M-315 49 Rowden 7 M-315 72 Rowden 86 65 Rowden 6 M-315 75 Rowden 1 M-315 51 Rowden 0 M-315 88 Rowden 3 M-315 70 Rowden 0 M-315 80 Rowden 0 M-315 36 Rowden 8 M-315 55 Rowden 3 M-315 61 Rowden 16 M-315 29 Rowden 13 M-315 66 Rowden 5 M-315 64 Rowden 3 M-315 86 Rowden 1 M-315 80 Rowden 6 M-315 68 Rowden 13 M-315 49 Rowden 6 M-315 4 Rowden 6 M-315 20 Rowden 0 M-315 48 Rowden 21 M-315 89 Rowden 14 M-315 94 Rowden 18 M-315 38 Rowden 17 M-315 119 0 M-315 68 Rowden 2 M-315 47 Rowden 3 M-315 75 Rowden 5 M-315 97 Rowden 0 M-315 38 Rowden M-315 41 Rowden 10 M-315 7 Rowden 4 M-315 70 Rowden 5 M-315 24 Rowden 8 M-315 77 Rowden 8 M-315 61 Rowden 11 6 rows 8 rows 8 rows 8 rows 275 250 N 6 rows 2001 NATIONAL COTTON FUSARIUM WILT REPORT 5 2001 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Percent wilt per replicate Entry Cultivar/Line Rep1 Rep2 Rep3 Rep4 C. Wayne Smith, Texas A&M University, 2474 TAMUS, College Station, TX 77843-2474 101 1 3 3 8 0 102 2 88 51 57 42 103 3 45 80 38 83 104 4 45 79 61 15 105 5 16 7 2 16 106 6 26 57 47 68 107 7 3 11 55 48 108 8 0 7 16 49 Rowden 81 100 49 24 M-315 2 0 13 14 Luther Bird, G & P Seed Co., 729 Shady Lane, Bryan, TX 77802 201 1 13 25 5 8 202 2 6 13 50 18 203 3 25 4 8 22 204 4 20 14 16 22 205 5 0 17 24 25 206 6 8 25 10 28 207 7 12 5 9 29 208 8 22 16 8 5 Rowden 87 94 36 61 M-315 5 6 4 17 Fred Bourland, University of Arkansas, P.O. Box 48, Keiser, AR 72351 301 ARK-1 0 14 3 0 302 ARK-2 0 9 11 13 303 ARK-3 1 14 26 9 304 ARK-4 1 22 3 12 305 ARK-5 6 53 3 15 306 ARK-6 27 38 79 71 307 ARK-7 55 69 84 25 308 ARK-8 10 18 8 12 Rowden 74 67 36 4 M-315 7 9 0 11 Don Keim, Delta and Pine Land Co., 100 Main Street, Scott, MS 38772 401 1 15 7 6 92 402 2 13 4 0 9 403 3 46 26 17 27 404 4 16 44 12 2 405 5 54 24 32 32 406 6 8 29 8 9 407 7 42 17 28 27 408 8 12 19 21 13 Rowden 75 82 66 70 M-315 6 2 0 21 Avg. 3 60 62 50 10 49 29 18 64 7 13 22 14 18 17 18 13 13 69 8 4 8 12 10 19 54 58 12 45 7 30 7 29 18 36 14 29 16 73 7 continued 6 ALABAMA AGRICULTURAL EXPERIMENT STATION 2001 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Percent wilt per replicate Entry Cultivar/Line Rep1 Rep2 Rep3 Rep4 Avg. Frank Bordelon, PhytoGen Cottonseed, P.O. Box 27, Leland, MS 38756 501 PHY-FB1 61 53 17 70 50 502 PHY-FB2 36 7 17 38 25 503 PHY-FB3 9 10 16 10 11 504 PHY-FB4 73 17 46 9 37 505 PHY-FB5 1 6 0 10 4 506 PHY-FB6 20 40 53 43 39 507 PHY-FB7 1 7 6 13 7 508 PHY-FB8 11 3 7 11 8 Rowden 69 87 75 38 67 M-315 4 8 1 6 5 Randall McPherson, PhytoGen Cottonseed, P.O. Box 27, Leland, MS 38756 601 PHY-RM1 0 5 11 6 6 602 PHY-RM2 2 10 9 6 7 603 PHY-RM3 7 22 13 5 12 604 PHY-RM4 16 15 21 9 15 605 PHY-RM5 9 0 9 4 5 606 PHY-RM6 5 9 9 13 9 607 PHY-RM7 8 0 13 17 10 608 PHY-RM8 25 13 8 11 14 Rowden 82 51 66 41 60 M-315 18 7 7 6 9 Gary L. Rea, Delta and Pine Land Co., 1303 N. Avenue I, Haskell, TX 79521 701 GLR 1 16 50 18 15 25 702 GLR 2 9 6 5 10 8 703 GLR 3 36 13 17 15 20 704 GLR 4 6 1 7 2 4 705 GLR 5 7 9 8 9 8 706 GLR 6 3 3 15 12 8 707 GLR 7 56 31 31 28 36 708 GLR 8 26 17 45 13 25 Rowden 74 89 75 68 77 M-315 7 12 16 3 9 Peggy Thaxton, Texas A&M University, Soil & Crop Sciences, College Station, TX 77843-2474 801 PMT-1 14 6 9 6 9 802 PMT-2 56 18 10 16 25 803 PMT-3 29 45 18 16 27 804 PMT-4 6 7 11 2 6 805 PMT-5 17 13 3 19 13 806 PMT-6 5 30 17 26 19 807 PMT-7 3 19 14 22 14 808 PMT-8 17 63 2 11 23 Rowden 69 95 80 7 63 M-315 4 9 0 0 3 continued 2001 NATIONAL COTTON FUSARIUM WILT REPORT 7 2001 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Percent wilt per replicate Entry Cultivar/Line Rep1 Rep2 Rep3 John Green, Seed Source, Inc., P.O. Box 28, Stoneville, MS 38776 901 SSI-1 3 19 13 902 SSI-2 25 4 24 903 SSI-3 0 18 0 904 SSI-4 14 40 17 905 SSI-5 44 7 27 906 SSI-6 74 2 50 907 SSI-7 46 0 56 908 SSI-8 7 15 0 Rowden 76 35 45 M-315 6 5 3 Richard Sheetz, Delta and Pine Land Co., RR 2, Box 60, Hale Center, TX 79041 1001 RS-1 11 0 7 1002 RS-2 4 7 24 1003 RS-3 6 19 8 1004 RS-4 1 4 7 1005 RS-5 3 4 6 1006 RS-6 1 7 14 1007 RS-7 54 17 20 1008 RS-8 1 5 11 Rowden 75 91 48 M-315 2 12 1 Ted Wallace, Mississippi State University, P.O. Box 9555, Starkville, MS 39762 1101 TPW1 13 12 0 1102 TPW2 9 3 21 1103 TPW3 26 11 10 1104 TPW4 8 11 0 1105 TPW5 14 21 0 1106 TPW6 31 5 0 1107 TPW7 14 14 6 1108 TPW8 24 9 24 Rowden 97 36 64 M-315 1 4 8 O. Lloyd May, University of Georgia, P.O. Box 748, Tifton, GA 31793-0748 1201 GA96-54 4 0 8 1202 GA96-199 9 16 43 1203 GA96-211 2 11 32 1204 GA97-5 24 24 16 1205 GA97-8 38 0 7 1206 GA97-14 14 12 27 1207 GA97-23 0 0 26 1208 GA98084 16 10 14 Rowden 62 33 51 M-315 0 4 0 Rep4 3 91 35 13 30 6 0 10 20 10 6 14 7 6 3 6 14 1 47 1 0 14 13 0 20 33 27 30 38 8 5 38 6 4 26 30 13 13 97 13 Avg. 10 36 13 21 27 33 25 8 44 6 6 12 10 5 4 7 26 4 66 4 6 12 15 5 14 17 15 22 59 5 4 27 13 17 18 21 10 13 61 4 continued 8 ALABAMA AGRICULTURAL EXPERIMENT STATION 2001 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Percent wilt per replicate Entry Cultivar/Line Rep1 Rep2 Rep3 Rep4 Avg. Jack E. Jones, Jajo Genetics, 246 Maxine Dr., Baton Rouge, LA 70808-6831 1301 Jajo 1 22 15 63 12 28 1302 Jajo 2 6 15 8 0 7 1303 Jajo 3 19 16 20 4 15 1304 Jajo 4 1 25 28 22 19 1305 Jajo 5 17 13 20 9 15 1306 Jajo 6 4 21 11 6 11 1307 Jajo 7 44 27 35 5 28 1308 Jajo 8 4 8 59 13 21 Rowden 79 79 61 64 71 M-315 4 5 4 0 3 Dawn Fraser, Delta and Pine Land Co., P.O. Box 1529, Hartsville, SC 29551 1401 1 16 29 55 11 28 1402 2 42 50 16 35 36 1403 3 17 18 23 13 17 1404 4 54 2 3 40 25 1405 5 8 6 20 18 13 1406 6 8 17 10 9 11 1407 7 76 67 85 72 75 1408 8 14 59 54 22 37 Rowden 86 91 88 75 85 M-315 0 24 3 6 8 Douglas Wessel, Delta and Pine Land Co., 38768 W. Farrell Rd., Maricopa, AZ 85239 1501 DW-1 96 100 88 100 96 1502 DW-2 3 10 47 8 17 1503 DW-3 29 12 8 6 14 1504 DW-4 74 97 88 91 87 1505 DW-5 2 5 35 15 14 1506 DW-6 9 32 22 6 17 1507 DW-7 4 10 3 3 5 Daryl Bowman, NC State University,Crop Science Dept, Box 8604, Raleigh, NC 27695-8604 1508 NC98-34 11 18 63 41 33 Rowden 77 79 72 80 77 M-315 0 4 5 3 3 Michael Swindle, Aventis Cotton Seed Inter.,117 Kennedy Flat Road, Leland, MS 38756 1601 ACSI-1 2 6 0 9 4 1602 ACSI-2 12 0 9 55 19 1603 ACSI-3 16 6 14 50 21 1604 ACSI-4 9 21 9 20 15 1605 ACSI-5 6 22 0 12 10 1606 ACSI-6 53 16 19 22 27 1607 ACSI-7 16 4 11 18 12 1608 ACSI-8 1 0 2 8 3 Rowden 96 14 19 77 51 M-315 1 3 0 18 6 continued 2001 NATIONAL COTTON FUSARIUM WILT REPORT 9 2001 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Percent wilt per replicate Entry Cultivar/Line Rep1 Rep2 Rep3 Rep4 Lloyd McCall, Stoneville Pedigreed Seed Co., Leland, MS 1701 LM1 2 0 14 14 1702 LM2 0 33 17 19 1703 LM3 63 16 36 43 1704 LM4 46 6 15 16 1705 LM5 14 3 7 76 1706 LM6 16 10 22 19 1707 LM7 2 21 11 16 1708 LM8 0 10 0 19 Rowden 96 46 65 68 M-315 3 39 0 5 Mark Barfield, Stoneville Pedigreed Seed Co., 2409 Commerce Lane, Albany, GA 31707 1801 MB1 4 88 44 58 1802 MB2 89 24 31 22 1803 MB3 33 58 3 25 1804 MB4 36 20 41 55 1805 MB5 23 36 55 9 1806 MB6 14 23 27 32 1807 MB7 34 10 15 27 1808 MB8 3 8 11 45 Rowden 79 95 29 94 M-315 10 0 3 8 Mike Robinson, Stoneville Pedigreed Seed Co., Leland, MS 1901 MR1 35 55 66 35 1902 MR2 8 7 7 9 1903 MR3 7 32 7 11 1904 MR4 9 0 9 3 1905 MR5 27 27 8 3 Paul Fox, Stoneville Pedigreed Seed Co., Memphis, TN 1906 PF1 39 6 81 57 1907 PF2 24 6 13 34 1908 PF3 42 0 78 36 Rowden 89 82 78 86 M-315 3 1 6 2 Steve Calhoun, Stoneville Pedigreed Seed Co., Idalou, TX 2001 SC1 20 0 13 0 2002 SC2 11 4 16 13 2003 SC3 2 0 2 12 2004 SC4 19 16 5 14 2005 SC5 9 3 13 10 2006 SC6 25 19 6 45 2007 SC7 6 18 13 27 2008 SC8 45 34 50 52 Rowden 90 85 70 49 M-315 1 4 4 0 Avg. 7 17 39 21 25 17 12 7 69 12 49 41 30 38 31 24 21 17 74 5 48 8 14 5 16 46 19 39 84 3 8 11 4 13 8 24 16 45 73 2 continued 10 ALABAMA AGRICULTURAL EXPERIMENT STATION 2001 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, AL Percent wilt per replicate Entry Cultivar/Line Rep1 Rep2 Rep3 Rep4 Randy Wood, Stoneville Pedigreed Seed Co., Maricopa, AZ 2101 RW1 6 10 13 16 2102 RW2 19 70 87 63 2103 RW3 25 87 34 38 2104 RW4 1 15 13 18 2105 RW5 11 21 23 0 2106 RW6 7 11 7 7 2107 RW7 30 39 49 63 2108 RW8 13 57 71 20 Rowden 43 90 26 48 M-315 4 2 3 4 Kathryn M. Glass, Dept. of Agronomy & Soils, Auburn University, AL 36849-5412 2201 Fiber Max FM 966 44 40 8 29 2202 PhytoGen PSC 355 22 17 15 9 2203 Stoneville ST 4892BR 31 44 24 34 2204 Deltapine DP 565 9 9 0 7 2205 Deltapine DP 422 B/RR 6 5 17 15 2206 Deltapine DP 655B/RR 0 9 23 0 2207 Sure Grow 215 BG/RR 5 6 28 5 2208 Paymaster PM 1199RR 7 5 8 41 Rowden 75 94 55 89 M-315 17 10 19 5 Avg. 11 60 46 12 14 8 45 40 52 3 30 16 33 6 11 8 11 15 78 13