2000 National Cotton Fusarium Wilt Report NOVEMBER 2000 AGRONOMY AND SOILS DEPARTMENT SERIES NO. 231 ALABAMA AGRICULTURAL EXPERIMENT STATION LUTHER WATERS, JR., DIRECTOR AUBURN UNIVERSITY AUBURN, AL 36849 THIS REPORT IS A JOINT CONTRIBUTION BETWEEN USDA-ARS, CROP SCIENCE RESEARCH LABORATORY, MISSISSIPPI STATE UNIVERSITY, MISSISSIPPI, AND THE ALABAMA AGRICULTURAL EXPERIMENT STATION, AUBURN UNIVERSITY, ALABAMA Information contained herein is available to all persons regardless of race, color, sex, or national origin 2000 NATIONAL COTTON FUSARIUM WILT REPORT 3 2000 NATIONAL COTTON FUSARIUM WILT REPORT Kathryn M. Glass1, William S. Gazaway2, and Edzard van Santen 3 1,3 Agricultural Program Associate and Associate Professor, respectively, Dept. of Agronomy and Soils, Auburn University, AL 36849 2 Professor Emeritus and Extension Pathologist/Nematologist, Dept. of Entomology and Plant Pathology, Auburn University, 36849 Cotton cultivars and elite breeding lines submitted by 25 cooperators were evaluated for Fusarium wilt resistance under field conditions at the E. V. Smith Research Center, Plant Breeding Unit, Tallassee, Alabama. These entries were grown on an Independence loamy fine sand highly infested with the Fusarium wilt fungus (Fusarium oxysporum) Schlect. f. vasinfectum [Atk.] (Snyd. & Hans.) and southern root-knot nematodes (Meloidogyne incognita.). In 1994, a soil analysis for nematodes revealed that southern root-knot (Meloidogyne incognita) and lance (Hoplolaimus galeatus) are the predominant nematode species in the test plots. High populations of both species are found throughout the test area. Other nematode genera present are stubby root (Trichodorus sp.) and stunt (Tylenchorhynchus sp.). Root-knot nematodes, however, appear to be causing the major damage to cotton in the Fusarium Wilt Test as indicated by the high galling indices found on the roots of all cotton lines. Entries were planted in single 20-foot rows on 40-inch centers, separated by 5-foot alleys. Four replications of the test entries and checks were evaluated in a randomized complete block design with a split plot restriction on randomization. The set of eight test cultivars submitted by a cooperator was always evaluated as a group together with two control plots within each replicate. Both susceptible (Rowden) and resistant (M-315) cultivars were included as check subplots in the two center rows of each main plot (Fig. 1). Initial plant counts were made on June 21. Wilted plants were counted and removed on June 30 and July 26. The remaining live plants were counted and recorded on August 16. Total percent wilted plants were then determined and mean wilting for a given entry calculated. The average % wilted plants for the susceptible check Rowden was 43%, with a range from 3 to 95% on an individual plot basis (Fig. 1). The resistant check M-315 had, on the average, 8% wilted plants with a range from 0 to 25% on an individual plot basis. There were, on the average, 5x more wilted plants in Rowden plots than in M-315 plots. Critical evaluations of breeding lines should be made relative to the two checks listed at the bottom of each group. ALABAMA AGRICULTURAL EXPERIMENT STATION Fig. 1. Field layout, plot distances and % wilt for control plots of Rowden (susceptible) and M-315 (resistant). The numbers in the column marked N-S and in the row marked E-W are the distances from the Northeast corner of the trial to a particluar tier or row. N-S Rowden M-315 Rowden M-315 Rowden M-315 Rowden M-315 Rowden M-315 475 B 67 79 78 69 63 37 51 54 74 65 60 91 95 90 72 53 74 82 B B 4 plots 81 71 20 9 6 6 8 7 17 3 4 4 plots 4 plots 15 0 25 0 5 36 38 41 44 50 44 12 20 17 30 11 28 31 53 11 55 0 0 4 1 21 13 1 1 0 11 7 1 11 6 4 plots 4 plots 12 40 0 2 29 19 10 9 1 1 27 0 6 7 5 18 450 B 425 B 400 B 375 B 350 B 325 B 300 B 275 B B B B B B B B B B B B B B B B B B B 9 6 33 19 11 14 26 30 33 53 41 20 44 42 36 48 50 27 35 50 24 29 87 12 9 0 8 25 9 9 15 11 17 17 18 24 11 20 20 15 7 4 4 4 plots 4 plots 23 12 35 8 55 37 50 63 16 63 67 74 70 59 71 75 58 54 74 90 120 0 12 6 7 11 5 9 15 4 5 8 18 8 9 9 7 9 0 8 7 21 14 B B 15 3 B B 47 2 B B 15 4 B B 23 3 B B 14 1 B B 20 1 B B 17 4 B B 3 0 B B N 250 B 225 B 200 B 175 B 150 B 125 B 100 B 75 B 50 B 25 B 0 B E-W 0 23 10 B B 24 9 B B 43 2 B B 23 1 B B 69 7 B B 80 12 B B 61 8 B B 32 17 B B 7 6 B B 35 6 B B 4 plots 4 plots 24 153 9 4 plots B B 193 4 2000 NATIONAL COTTON FUSARIUM WILT REPORT 5 2000 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, Alabama Percent wilt per replicate Entry Cultivar/line Rep 1 Rep 2 Rep 3 Rep 4 Avg. Roy C. Creech, USDA-ARS, Mississippi State, MS 39762 101 1 25 20 27 7 20 102 2 36 7 39 24 27 103 3 23 1 17 1 11 104 4 2 1 61 6 18 105 5 11 10 26 12 15 106 6 33 4 30 9 19 107 7 37 12 6 3 15 108 8 25 18 38 2 21 Rowden 74 80 20 21 49 M-315 8 12 17 14 13 Jim Mitchell, Paymaster Cottonseed, 2476 Hwy. 130 South, Stuttgart, AR 72160 201 JM-1 6 42 45 84 44 202 JM-2 52 14 13 84 41 203 JM-3 69 54 38 48 52 204 JM-4 20 16 11 23 17 205 JM-5 33 20 62 100 54 206 JM-6 55 32 57 11 39 207 JM-7 29 55 19 31 34 208 JM-8 16 30 34 77 39 Rowden 7 20 40 79 36 M-315 6 0 0 6 3 Laval M. Verhalen, Oklahoma State University, 368 Agricultural Hall, Stillwater, OK 74078 301 OKLA-1 7 15 46 15 21 302 OKLA-2 14 29 33 18 23 303 OKLA-3 7 37 27 6 19 304 OKLA-4 4 12 30 11 14 305 OKLA-5 3 8 16 4 8 306 OKLA-6 36 23 5 7 18 307 OKLA-7 20 17 28 4 17 308 OKLA-8 23 18 51 1 23 Rowden 24 23 41 23 28 M-315 9 1 11 3 6 Don Keim, Delta and Pine Land Co., 100 Main Street, Scott, MS 38772 401 DeltaPearl 80 31 1 45 39 402 99X02 2 4 29 3 9 403 99X16 100 24 30 10 41 404 99X35 69 38 9 29 36 405 99X38 96 37 14 16 41 406 99X49 73 2 6 8 22 407 99X36 67 22 8 18 29 408 99X40 21 6 12 27 17 Rowden 71 12 17 23 31 M-315 4 1 4 0 2 continued 6 ALABAMA AGRICULTURAL EXPERIMENT STATION 2000 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, Alabama Percent wilt per replicate Entry Cultivar/line Rep 1 Rep 2 Rep 3 Rep 4 Avg. Charles G. Cook, Novartis, P.O. Box 1149, Santa Rosa, TX 78593 501 CGC-1 56 27 100 24 52 502 CGC-2 35 91 30 10 41 503 CGC-3 31 76 95 40 61 504 CGC-4 . 44 . 10 27 505 CGC-5 25 . 100 0 42 506 CGC-6 24 89 97 60 68 507 CGC-7 . . . . . 508 CGC-8 23 30 76 54 46 Rowden 54 95 74 26 62 M-315 0 9 0 25 8 Randall McPherson, PhytoGen Cottonseed, P.O. Box 27, Leland, MS 38756 601 PHX-1 32 45 13 8 25 602 PHX-2 72 71 21 3 42 603 PHX-3 52 35 14 13 28 604 PHX-4 85 31 15 9 35 605 PHX-5 62 21 8 3 24 606 PHX-6 100 27 43 25 49 607 PHX-7 19 26 26 18 22 608 PHX-8 100 63 1 5 42 Rowden 74 48 16 12 37 M-315 7 11 4 12 9 Gary L. Rea, Delta and Pine Land Co., 1301 East 50th St., Lubbock, TX 79401 701 00H01 4 36 16 20 19 702 00H02 25 12 2 3 10 703 00H03 14 37 17 25 23 704 00H04 52 26 9 38 31 705 00H05 34 21 10 40 26 706 00H08 31 44 25 1 25 707 00H38 8 14 38 32 23 708 00H39 15 48 16 19 25 Rowden 58 36 41 7 35 M-315 9 24 1 5 10 Peggy Thaxton, Texas A&M University, Soil & Crop Sciences, College Station, TX 77843 801 PMT-1 80 49 8 28 41 802 PMT-2 9 6 19 83 29 803 PMT-3 81 48 33 97 65 804 PMT-4 86 30 31 91 59 805 PMT-5 100 6 13 42 40 806 PMT-6 22 16 10 19 17 807 PMT-7 7 17 17 29 17 808 PMT-8 61 33 2 89 47 Rowden 81 43 23 67 53 M-315 3 2 10 9 6 continued 2000 NATIONAL COTTON FUSARIUM WILT REPORT 7 2000 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, Alabama Percent wilt per replicate Entry Cultivar/line Rep 1 Rep 2 Rep 3 Rep 4 Avg. John Green, Seed Source, Inc., P.O. Box 28, Stoneville, MS 38776 901 FW-1 100 42 18 75 59 902 FW-2 62 18 12 73 41 903 FW-3 29 28 32 48 34 904 FW-4 90 26 40 32 47 905 FW-5 97 14 27 3 35 906 FW-6 86 43 71 31 58 907 FW-7 22 13 35 91 40 908 FW-8 100 7 19 32 40 Rowden 53 44 53 78 57 M-315 8 1 15 1 6 Richard Sheetz, Delta and Pine Land Co., RR 2, Box 60, Hale Center, TX 79041 1001 1 29 5 19 0 13 1002 2 12 18 47 16 23 1003 3 25 10 51 32 29 1004 4 11 10 72 5 24 1005 5 15 21 19 11 16 1006 6 73 7 5 1 21 1007 7 20 54 9 6 22 1008 8 4 0 37 13 13 Rowden 24 44 37 47 38 M-315 4 21 12 2 10 Terry Weesner, Delta and Pine Land Co., P.O. Box 589, Maricopa, AZ 85239 1101 TW-1 3 26 25 26 20 1102 TW-2 22 58 26 20 32 1103 TW-3 18 34 15 6 18 1104 TW-4 19 7 26 15 17 1105 TW-5 13 27 4 14 14 1106 TW-6 8 32 16 25 20 1107 TW-7 38 11 23 27 25 1108 TW-8 20 14 4 5 11 Rowden 90 67 63 11 58 M-315 7 8 5 0 5 O. Lloyd May, University of Georgia, P.O. Box 748, Tifton, GA 31793-0748 1201 PD97006 22 17 16 8 16 1202 PD97019 33 68 38 16 39 1203 PD97021 41 100 10 6 39 1204 PD97047 16 76 43 2 34 1205 PD97072 8 64 17 24 28 1206 PD97100 22 38 45 18 31 1207 PD97101 43 53 22 19 34 1208 GA95200 26 25 6 7 16 Rowden 35 60 37 15 37 M-315 15 0 5 4 6 continued 8 ALABAMA AGRICULTURAL EXPERIMENT STATION 2000 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, Alabama Percent wilt per replicate Entry Cultivar/line Rep 1 Rep 2 Rep 3 Rep 4 Avg. Jack E. Jones, Jajo Genetics, 246 Maxine Dr., Baton Rouge, LA 70808-6831 1301 Jajo 1 21 96 0 23 35 1302 Jajo 2 13 12 27 16 17 1303 Jajo 3 68 75 7 32 46 1304 Jajo 4 78 79 4 32 48 1305 Jajo 5 31 92 29 1 38 1306 Jajo 6 49 38 4 40 32 1307 Jajo 7 12 88 2 68 42 1308 Jajo 8 7 30 11 35 21 Rowden 75 91 20 55 60 M-315 7 15 1 11 8 Cindy Green, Delta and Pine Land Co., P.O. Box 1529, Hartsville, SC 29551 1401 1 36 20 36 26 30 1402 2 28 40 44 41 39 1403 3 35 29 27 82 43 1404 4 31 40 27 35 33 1405 5 48 10 35 48 35 1406 6 49 34 9 33 31 1407 7 8 14 41 45 27 1408 8 67 52 31 27 44 Rowden 11 59 38 33 35 M-315 1 9 4 12 7 Douglas Wessel, Delta and Pine Land Co., 1305 N. VIP Blvd., Casa Grande, AR 85222 1501 DW-1 33 9 41 49 33 1502 DW-2 79 44 47 100 67 1503 DW-3 30 62 34 96 56 1504 DW-4 55 38 7 98 50 1505 DW-5 36 51 70 60 54 1506 DW-6 38 66 30 19 38 1507 DW-7 64 91 91 49 74 1508 DW-8 0 12 21 29 16 Rowden 29 71 33 69 51 M-315 4 9 9 10 8 Michael Swindle, AgrEvo Cotton Seed Inter, 4301-A, Hwy 82 East, Greenville, MS 38703 1601 FM 819 53 19 9 67 37 1602 FM 832 28 28 17 94 42 1603 FM 958 51 22 5 71 37 1604 FM 966 25 11 19 21 19 1605 FM 989 13 31 28 4 19 1606 ACSI EXPIF1000 6 12 14 62 24 1607 ACSI EXP0781 31 0 25 9 16 1608 ACSI EXP0223 0 12 3 93 27 Rowden 32 24 14 63 33 M-315 17 9 1 2 7 continued 2000 NATIONAL COTTON FUSARIUM WILT REPORT 9 2000 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, Alabama Percent wilt per replicate Entry Cultivar/line Rep 1 Rep 2 Rep 3 Rep 4 Avg. Lloyd McCall, Stoneville Pedigreed Seed, 2409 Commerce Lane, Albany, GA 31707 1701 LM1 62 45 24 14 36 1702 LM2 100 97 32 25 63 1703 LM3 14 50 47 7 30 1704 LM4 94 35 8 30 42 1705 LM5 6 48 16 29 25 1706 LM6 82 69 23 53 57 1707 LM7 63 15 68 20 42 1708 LM8 8 17 25 9 15 Rowden 82 44 55 14 48 M-315 17 17 0 8 11 Mark Barfield, Stoneville Pedigreed Seed, 2409 Commerce Lane, Albany, GA 31707 1801 MB1 23 38 100 1 40 1802 MB2 22 44 61 1 32 1803 MB3 49 75 100 4 57 1804 MB4 59 77 33 0 42 1805 MB5 24 18 92 17 38 1806 MB6 18 29 51 7 26 1807 MB7 33 18 77 8 34 1808 MB8 12 27 3 8 12 Rowden 27 74 65 15 45 M-315 20 18 25 3 16 Mike Robinson, Stoneville Pedigreed Seed, 2409 Commerce Lane, Albany, GA 31707 1901 MR1 34 90 11 36 43 1902 MR2 35 84 8 45 43 1903 MR3 54 36 18 29 34 1904 MR4 19 4 0 34 15 1905 MR5 22 46 8 40 29 1906 MR6 44 77 21 11 38 1907 MR7 27 100 14 40 45 1908 MR8 23 75 4 0 25 Rowden 50 72 3 27 38 M-315 7 6 0 0 3 Steve Calhoun, Stoneville Pedigreed Seed, 2409 Commerce Lane, Albany, GA 31707 2001 SC1 76 22 24 52 43 2002 SC2 47 11 89 24 43 2003 SC3 40 26 36 22 31 2004 SC4 50 20 33 25 32 2005 SC5 29 8 58 37 33 2006 SC6 33 26 97 64 55 2007 SC7 15 16 83 40 39 2008 SC8 32 26 94 25 44 Rowden 17 50 51 29 37 M-315 11 13 11 19 13 continued 10 ALABAMA AGRICULTURAL EXPERIMENT STATION 2000 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, Alabama Percent wilt per replicate Entry Cultivar/line Rep 1 Rep 2 Rep 3 Rep 4 Avg. Randy Wood, Stoneville Pedigreed Seed, 2409 Commerce Lane, Albany, GA 31707 2101 RW1 29 30 17 19 24 2102 RW2 40 5 3 4 13 2103 RW3 13 54 40 35 36 2104 RW4 35 18 9 21 21 2105 RW5 45 38 0 11 24 2106 RW6 11 40 23 43 29 2107 RW7 42 22 34 57 39 2108 RW8 13 12 14 15 13 Rowden 30 69 50 9 39 M-315 7 7 9 1 6 Jody Butler, Stoneville Pedigreed Seed, 2409 Commerce Lane, Albany, GA 31707 2201 JB1 28 34 89 39 47 2202 JB2 7 32 95 32 41 2203 JB3 30 69 36 36 43 2204 JB4 21 27 90 39 44 2205 JB5 32 38 35 8 28 2206 JB6 33 31 82 32 44 2207 Rowden 25 65 92 50 58 2208 M-315 4 0 0 8 3 Rowden 35 42 54 19 38 M-315 6 18 5 9 9 Ted Wallace, Mississippi State University, Starkville, MS 39762 2301 1 44 23 12 36 29 2302 2 60 55 19 21 39 2303 3 44 25 17 26 28 2304 4 15 27 8 22 18 2305 5 31 22 30 3 22 2306 6 44 46 25 8 31 2307 Rowden 65 79 45 20 52 2308 M-315 0 21 23 6 12 Rowden 28 70 63 8 42 M-315 11 8 15 7 10 Curtis Williams, Paymaster Cottonseed, 2476 Hwy. 130 South, Stuttgart, AR 72160 2401 CW1 14 38 3 14 17 2402 CW2 4 11 62 25 25 2403 CW3 16 8 27 15 17 2404 CW4 4 44 18 8 19 2405 CW5 31 26 63 7 32 2406 CW6 38 16 13 5 18 2407 CW7 22 35 36 13 26 2408 CW8 32 28 34 23 29 Rowden 61 50 36 35 46 M-315 8 20 0 6 9 continued 2000 NATIONAL COTTON FUSARIUM WILT REPORT 11 2000 Fusarium Wilt Test, Plant Breeding Unit, EVSRC, Tallassee, Alabama Percent wilt per replicate Entry Cultivar/line Rep 1 Rep 2 Rep 3 Rep 4 Kathryn M. Glass, Dept. of Agronomy & Soils, Auburn University, AL 36849-5412 2501 Stoneville 474 79 49 41 32 2502 AP 7126 36 87 36 10 2503 Sure-Grow 747 42 37 15 73 2504 Sure-Grow 821 28 65 14 13 2505 Deltapine DP 655B/RR 24 16 39 23 2506 Deltapine DP 675 20 18 14 24 2507 Deltapine NuCotn 33B 14 78 32 6 2508 Paymaster PM1218BG/RR 37 83 55 16 Rowden 31 90 30 18 M-315 6 6 9 6 Avg. 50 42 42 30 26 19 33 48 42 7