1 t 'N It 9 4 Id CONTENTS Page INTRODUCTION ................................................. ..................... 3 MATERIALS AND METHODS ................................. .................... 3 RESULTS AND DISCUSSION .................................. ..................... 5 SUMMARY ........................................... ..................... 7 LITERATURE CITED .......................................... ..................... 8 FIRST PRINTING, 3.5M, DECEMBER 1985 Information contained herein is available to all persons without regard to race, color, sex, or national origin. Influence of Galecron and Pix on cotton yield C. E. Snipes, R. H. Walker, and G. A. Buchanan INTRODUCTION was first introduced as an ovicide for fruit crops, vegetables, and cotton. In addition, yield enhancement of cotton was reported in the mid-1970's (6) and since then other researchers (2,9) have reported similar findings. Cotton yield increases ranged from 9 to 28 percent in field studies and as high as 39 percent in a greenhouse study. Galecron rate ranged from 1/8 to 1/2 pound per acre applied 6 to 9 times at weekly intervals, generally beginning at the pin-head square stage. However, reports have also been published that failed to show cotton yield enhancement with Galecron (1). Therefore, the primary objective of this study was to determine the usefulness of Galecron as a yield enhancer in a major cotton producing area of Alabama. A secondary objective was to evaluate the effectiveness and potential need for Pix ®. Pix is a foliar-applied chemical which modifies cotton growth and reproduction. It has been shown (3,5,7,8,10) to produce one or more of the following effects in cotton: darker leaf color, reduction of internode length, more open canopy, better boll retention, increased yield, increased water-stress resistance, and earlier maturity. MATERIALS AND METHODS Field experiments were conducted for 3 years (1980-82) on a Lucedale fine sandy loam (Rhodic Paleudult) at Prattville, Alabama. Cotton, Deltapine 61, was planted with conventional equipment in late April each year, table 1. Plots were 12 rows wide and 25 feet long, with a row width of 42 inches. Lime and fertilizer were applied according to recommendations of 'The authors are former Research Associate, Associate Professor, and Professor, respectively, of Agronomy and Soils. GALECRON® TABLE 1. APPLICATION DATES OF GALECRON AND PIX FOR 1980, 1981, AND 1982 Date 1981 4-20 7-15 7-15 1982 Planting ..................................................... 4-30 appicaion............................................... Pix application 7-9 7-9 Galecron application (1st) ..................................... 6-12 6-18 6-11 (2nd) ...................................................... 6-18 6-25 6-18 (3rd) ....................................................... 6-26 7-1 6-24 (4th) ....................................................... 7-2 7-8 7-1 (5th) ........................................................... 7-8 7-14 7-7 (6th) ........................................................... 7-15 7-20 7-13 Insecticide application (1st) ............................................................ 7-28' 7-7 7-17 (last) ........................................................... 8-29 9-3 9-3 Harvest (1st) ............................................................ 10-8 9-29 9-28 (2nd) .......................................................... 11-5 11-11 11-5 'Insecticides for bollworm control only; six applications of Dimilin applied from June 10 to July 14 for boll weevil control. Item 1980 4-25 7-9 the Auburn University Soil Testing Laboratory for optimum cotton production. Weed control was achieved by broadcast application of Prowl® (pendimethalin) and Cotoran® (fluometuron) at recommended rates and with cultivation as needed. Galecron applications were initiated at the pin-head square stage which was approximately 6 to 7 weeks after planting, table 1. The Galecron treatments consisted of two rates (1/8 and 1/4 pound per acre) applied three or six times. Application frequency was on 5- to 7-day intervals, table 1. Pix (0.04 pound per acre) was applied in a single application approximately 10 weeks after planting with and without Galecron, table 1. The Galecron treatments consisted of nine applications in 1980 and six in 1981 and 1982 at a rate of 1/8 pound per acre per application. A no-Galecron/no-Pix control treatment was also included. Treatments were arranged in a randomized complete block design with four replications. During the growing season, the entire experimental area was scouted regularly and treated for insects when necessary. Insecticides used most frequently and according to recommended rates and label specifications were Dimilin® (diflubenzuron) for boll weevil control, and Ambush® (permethrin), Lannate® (methomyl), and Azodrin® (monocrotophos) primarily as a larvicide and/or ovicide. EPN plus methyl parathion was used for boll weevil and bollworm control. Starting and ending dates for these applications are listed in table 1. These insecticides were applied in an effort to eliminate insects as a variable (4,6). [4] Cotton was machine-harvested twice yearly with a one-row cotton picker, table 1. Eight rows were harvested from each 12-row plot. Data for each harvest were summed to determine total seed cotton yield. All data were subjected to an analysis of variance and treatment differences were separated at the 0.05 level of probability using Duncan's multiple range test. RESULTS AND DISCUSSION First-harvest results showed some seed-cotton yield response tor treatments, table 2. The responses were not, however, consistent among years. Six applications of Galecron at 1/4 pound per acre per application in 1980 produced a significant yield increase (21 percent) when compared to the control. This same treatment in 1981 and 1982 showed a trend for higher first-harvest yield (7 to 10 percent), but the response was not statistically significant. Treatments that received three applications of Galecron at 1/4 pound per acre or those receiving three or six applications at 1/8 pound per acre produced first-harvest yield that was no different from the control treatment for all years. A single application of Pix (no Galecron) significantly reduced (23 percent) first-harvest yield in 1981, but yield was equivalent to the control treatment for the remaining years, table 2. This yield reduction did not occur when Pix was TABLE 2. INFLUENCE OF GALECRON AND PIX ON 1ST AND 2ND HARVEST OF SEED COTTON, 1980-82 reatment Seed cotton yield/acre l 1980 1981 1982 1st 2nd 1st 2nd 1st 2nd harvest harvest harvest harvest harvest harvest Lb. Lb. Lb. Lb. Lb. Lb. 1,500 b 107 a 1,750 b 902 a 2,044 c 2,160 bc 589 a 598 a Galecron .... 8 lb./acre .... 3 app., 'A lb./acre .... 3 app., 8 lb./acre 6 app., 1,571 b 1,732 a 1,286 c 107 a - 1,767 b 1,776 b 1,044 a 973 a 2,205 abc 527 a 2,383 a 2,124 c 553 a 661 a 6 app., A lb./acre .... Pix, I app.,0.041lb./acre Galecron , 6 app., 2 /8 107 a 125 a 2,017 a 1,410 c 902 a 919 a lb./ acre + Pix, 1 app., 0.04 lb./acre ............ 1,500 b 116 a 1,741 b 821 a 2,339 ab 643 a Control; no Galecron/no Pix ............................ 1,428bc 116 a 1,830ab 902ab 2,222 abc 678 a IMeans within a column followed by the same letter are not significantly different at 2the .05 probability level according to DRMT. Nine applications of Galecron at '/8 pound per acre per application were made in 1980. [5] applied to cotton that received six applications of Galecron at 1/8 pound per acre. The Galecron plus Pix treatment produced first-harvest yield equivalent to the control treatment for the remaining 2 years. No differences in secondharvest yield were detected among any Galecron and/or Pix treatments for any year, table 2. Total seed-cotton yield (first harvest plus second harvest) showed results similar to first-harvest yield data, table 3. Galecron applied six times at 1/4 pound per acre per application produced the highest total yield. Percent increase over the control treatment was 19, 7, and 1 percent for 1980, 1981, and 1982, respectively. Only the 1980 response was significant. Pix applied alone produced a total seed-cotton yield lower in 1980 (9 percent) and 1981 (15 percent) than the control treatment. Yield in 1983 was equivalent to the control, table 3. Rainfall decreased sharply in 1980 and 1981 after the Pix application, which indicates that dry weather and Pix have at least an additive effect in checking growth of cotton. When Pix was applied to cotton that received six applications of Galecron at 1/8 pound per acre per application, reduction in total seed-cotton yield was prevented in 1980 and partially prevented in 1981. The reason for this is not clear. In 1981 and 1982, each plot was monitored to determine if Galecron had an effect on bollworm egg populations. In 1981, egg populations were high in early July and decreased rapidly with time, table 4, but there were no differences in egg populations (P = 0.05) among treatments. In 1982, mulTABLE 3. TOTAL SEED COTTON YIELD AS INFLUENCED BY VARIOUS RATES AND APPLICATIONS OF GALECRON AND PIX, 1980-82 Treatment Galecron 3 app., '/slb./acre .................................... 6 app., '/8 lb./acre .................................... 3 app., 'A lb./acre .................................... lb./acre .................................... 6 app., Pix, 1 app., 0.04 lb./acre ............................ Galecron2, 6 app.,'/8 lb./acre + Pix, 1 app., 0.04 lb./acre ................................ Control; no Galecron, no Pix ...................... Seed cotton yield/acre' 1980 1981 1982 Lb. 1,607 1,607 t1,839 1,410 b b a c Lb. 2,651 2,812 2,749 2,919 2,330 ab ab ab a c Lb. 2,633 2,731 2,758 2,937 2,785 b ab ab ab ab 1,616 b 1,544 bc 2,562 bc 2,731 ab 2,981 a 2,901 ab 'Means within a column followed by the same letter are not significantly different at 2the .05 probability level according to DMRT. pound per acre per application were made in Nine applications of Galecron at '/s 1980. [61 TABLE 4. INFLUENCE OF GALECRON AND PIX ON THE PRESENCE OF BOLLWORM EGGS IN COTTON, 1981 Treatment Galecron Eggs/100 terminals, by sampling date July August 4 9 16 23 30 6 13 23 27 No. No. No.N No. o. No. No. No. 3 app., /8 lb./acre ...................... 36 39 30 6 app., V8 lb./acre ........................ 35 31 28 3 app.,1/ lb./acre ... ............... 44 40 32 6 app., 1/4 lb./acre ...................... 48 32 32 Pix. 1 app., 0.04 lb./acre .................. 34 35 15 Galecron, 6 app., /s lb./acre + Pix, I app., 0.04 lb./acre .......... 44 50 32 Control; no Galecron, no Pix ............ 26 46 19 15 9 9 18 18 9 11 9 6 5 5 4 6 11 4 5 4 6 5 4 5 2 2 2 2 2 2 2 6 5 6 6 5 4 6 3 3 2 2 2 1 3 tiple applications of EPN + methyl parathion were applied to control boll weevil infestations, and as a result, bollworm populations were low. These data show that bollworm control was eliminated as a variable. In this study, Galecron increased cotton yield in 1 of 3 years. It is believed this increase was due to effects other than insect control. However, until conditions are identified that will make it more consistent, the full potential for Galecron as a yield enhancer will not be realized. Pix (no Galecron) reduced cotton growth but also reduced cotton yield. Pix plus dry weather was probably responsible for detrimental yield effects. When Pix was applied to cotton that received Galecron, most detrimental yield effects were eliminated. Perhaps Galecron could be used as a tool to safeguard cotton yield where producers normally use Pix and are faced with variable weather patterns. SUMMARY Field studies were conducted for 3 years to determine the effects of Galecron (chlordimeform) and Pix (mepiquat chloride) on seed-cotton yield. Treatments included three and six applications of Galecron at rates of 1/8 and 1/4 pound per acre per application. The initial treatment was applied at the pin-head square stage, and subsequent applications were at 5to 7-day intervals. A single application of Pix (0.04 pound per acre) was applied during early bloom with and without six applications of Galecron (1/8 pound per acre per application). Cotton (Deltapine 61) was harvested mechanically twice each year. Six applications of Galecron at 1/4 pound per acre per [7] application increased yield of the first harvest in 2 of 3 years when compared to the no-Galecron control, but the other Galecron treatments (reduced rate or number of applications) did not affect yield. No differences in second-harvest yield were found among any treatments for any year. Total seed- cotton yield was increased by Galecron (1/4 pound per acre, six applications) in 1 of 3 years, but the other Galecron treatments (reduced rate or number) did not affect total yield. Pix alone reduced first-harvest yield in 1 year and total seed-cotton yield in 2 years, and failed to increase seed-cotton yield in any year. Yield with the Pix + Galecron treatment did not differ from the no-Galecron check. However, the Pix + Galecron treatment increased total yield over that of Pix alone in 1 of 3 years. LITERATURE CITED (1) (2) (3) D.L. AND V.S. HOUSE. 1978. Effects of Chlordimeform on Insects Associated with Cotton. Southwest. Entomol. 3:284-291. BULL, CAMPBELL, W.R., C.J. COUNSELMAN, H.W. RAY, AND L.J. TERRY. 1979. Evaluation of Chlordimeform (Galecron®) for Heliothis virescens Control on Cotton. Beltwide Cotton Prod. Res. Conf. pp. 122-125. COTHREN, J.T. 1979. "Pix" - A Cotton Growth Regulant. Ark. Farm Res. 28:5. (4) DURANT, J.A. 1977. Methomyl on Cotton: Evaluation of Use Patterns for Phytotoxicity and Efficacy Against the Bollworm and Tobacco Budworm. J. Econ. Entomol. 70:641-643. (5) HEILMAN, M.D. M 1981. Interactions of Nitrogen with Pix T on the Growth and Yield of Cotton. Beltwide Cotton Prod. Res. Conf. p. 47. (6) LINCOLN, C. AND G. DEAN. 1976. Yield and Blooming of Cotton as Affected by Insecticides. Ark. Farm Res. 25:5. (7) MAPLES, R. 1981. Effects of Pix and Varying Rates of Nitrogen on Cotton, 1980. Ark. Farm Res. 30:5. (8) NAMKEN, L.N. AND H.W. GAUSMAN. 1978. Practical Aspects of Chemical Regulation of Cotton Plant Growth and Fruiting. Beltwide Cotton Prod. and Mech. Conf. pp. 23-25. (9) PHILLIPS, J.R., G.A. HERZOG, AND W.F. NICHOLSON. 1977. Effect of Chlordimeform on Fruiting Characteristics and Yield of Cotton. Ark. Farm Res. 26:4. (10) WILLARD, J.I. AND R.H. KUPELINA. 1981. Effects of 1,1-DimethylPiperidinium Chloride (BAS 083 00 W) on Cotton Yield and Development. Beltwide Cotton Prod. Res. Conf. p. 69.