CIRCULAR 53 Dusting Cotton With Calcium Arsenate for Boll Weevil Control (SECOND PROGRESS REPORT) By J. M. ROBINSON, Entomologist And F. S. ARANT, Assistant Entomologist AGRICULTURAL EXPERIMENT STATION OF THE ALABAMA POLYTECHNIC INSTITUTE M. J. FUNCHESS, Director AUBURN MAY, 1929 Table of Contents Page IN TR O D U CTIO N ------------------------------------ EXPERIMENTAL METHOD ------------------ 3 NORFOLK SANDY LOAM PLOTS, 1926-1928-4 Infestation and Dusting ------------ ----- - 4 Yields-6 CECIL CLAY PLOTS, 1926-1928 ---- ----- - 8 Infestation and Dusting-8 Y ie ld s - - - - - - - - - - - - - - - -- - - -- - - - - - - - - - - - - - - - - - -- - 1 0 HOUSTON CLAY PLOTS, 1926-1928 ------------------- 10 Infestation and Dusting --------------------------- 10 Y ie ld s ------------- -- - ------------------------ 1 2 WHEN TO DUST AND WHEN NOT TO DUST COTTON AS SHOWN BY THE INFESTATION IN 1926 A N D 192 7 - - - - - - - - - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - 12 FIVE-YEAR AVERAGE YIELD OF SEED COTTON PER ACRE ON THREE SOIL TYPES --------------------- 13 SU M M A R Y ----------------------------------------- 14 Dusting Cotton With Calcium Arsenate for Boll Weevil Control (Second Progress Report) By J. M. Robinson, Entomologist and F. S. Arant, Assistant Entomologist COTTON dusting tests with calcium arsenate for the control of the boll weevil were made in 1924 and 1925 on three dis- tinct types of soil,-namely, Norfolk sandy loam at Auburn, Cecil clay in Lee County, and Houston clay in Montgomery Coun- ty. Detailed data of these tests were published in Circular 51. The tests were continued in 1926, 1927, and 1928 on the plots previously used, with one exeception. This exception occurred in 1926 on the Houston clay soil, when the tests were conducted on plots in a different part of the same field. This report includes the detailed cotton dusting data on the three soil types for the years 1926, 1927, and 1928. It also in- cludes the average infestation and the seed cotton yield for the five-year period from 1924 to 1928, inclusive. EXPERIMENTAL METHOD THE METHOD used in determining the infestation consisted of first definitely locating three to six stations in each of.the dusted and undusted plots. Each station was marked by a stake five feet long. The infestation was determined by ex- amining one hundred squares at each station. All the squares on a plant were counted and the number of punctured squares in one hundred was used as the per cent of infestation at a given station. When the average infestation of the plot reached 10 per cent, three applicaitons of calcium arsenate were made at inter- vals of four to six days. Further infestation counts were made at intervals of four to seven days following the third application of dust. When the in- festation approached 20 per cent, the cotton was again dusted to protect the newly-formed squares and the young bolls. This method of determining the time to dust the cotton was continued throughout the growing season or until the weevil migration caused complete infestation (75 per cent or above). The applications of calcium arsenate were made at different times of the day, varying from early morning to late evening, but at a time when the air was relatively calm. The condition of foliage varied from wet to dry. Applications of dust were re- 4 peated when there was a heavy rainfall within 24 hours follow- ing the regular dustings. The amount of calcium arsenate used in dusting an acre of cotton varied from six to seven pounds per application, with an average of 6.7 pounds. The number of applications of dust each season varied from three to eleven, depending upon the infesta- tion. All applications of calcium arsenate dust were made with a one-mule, two-row, power dust gun. The power gun was set to make a thin cloud of dust which settled over the entire plant, affording a thorough and even distribution of the dust particles. NORFOLK SANDY LOAM PLOTS, 1926-1928 THESE PLOTS were located at Auburn on the Agricultural Experiment Station farm. The soil had been in cultivation for many years. There were two series of seven one-tenth acre plots. Three of the plots of each series were not fertilized, -namely, 1, 4, and 7. The other four plots, 2, 3, 5, and 6 were treated respectively with 500, 1000, 1500, and 2000 pounds of fertilizer. One series of plots was not dusted, and the other series was dusted with calcium arsenate after the infestation reached 10 per cent. The dusted series of plots was alternated with the undusted series from year to year. The two series of plots were separated by a thirty-foot alley way, which was dust- ed but not fertilized. Infestation and Dusting The infestation and dusting records for the Norfolk sandy loam plots in 1926, 1927, and 1928 are recorded in Table 1. The infestation in 1926 did not reach 10 per cent until Au- gust 27 (Table 1). Three applications of calcium arsenate dust were then made at intervals of five to six days. The infestation on the dusted plots varied from 12 to 20 per cent from August 27, to September 14, while the infestation on the undusted plots gradually advanced from 15 to 57 per cent, during the same period. The low infestation on the dusted plots contrasted with the relatively high infestation on the undusted plots indicated a decided control of boll weevil. The early high infestation in 1927 continued throughout the growing season and was in striking contrast to the late infesta- tion in 1926 (Table 1). The continued high infestation in 1927 was apparently due to the twenty-eight days of June that were either cloudy or partly cloudy. The cloudy weather protected the pupae of the first generation so that a high percentage of them emerged as adults. On June 13 the infestation on the dust- ed plots was 11 per cent. Three dustings were made at inter- vals of three to five days, which resulted in the infestation drop- ping to 1 per cent July 1. In the meantime the infestation on the undusted plots had advanced to 28 per cent. The infestation was 15 per cent on the dusted plots July 15, while the infestation Table 1.-Infestation and Dusting Dates on Norfolk Sandy Loam Plots, Auburn, 1926, 1927, and 1928. 1926 Average Percent Infestation Date July 6 July 13 July 21 July 28 Aug. 5 Aug. 12 Aug. 23 Aug. 27 Aug. 31 Sept. 3 Sept. 14 Un- dusted 1.0 0.0 0.0 0.0 0.0 0.0 4.0 15.3 27.8 59.1 57.7 Dusted 0.0 0.0 0.5 0.0 0.0 0.0 4.0 13.0 12.3 17.7 20.3 Dusting Dates Aug. 28 Sept. 3 Sept. 8 *Rained within 24 hours. 1927 Average Percent Infestation Un- Date dusted Dusted June 9 7.1 0.0 June 13 4.0 11.7 June 20 5.4 8.8 June 28 15.0 4.2 July 1 28.7 1.2 July 5 25.2 2.2 July 8 35.2 2.2 July 12 47.2 5.1 July 15 36.2 15.4 July 19 39.2 14.5 July 22 55.1 11.7 July 26 74.1 8.7 July 29 83.1 14.5 Aug. 2 89.5 19.1 Aug. 6 88.0 83.8 Aug. 9 95.5 80.5 Aug. 12 92.2 87.7 Aug. 16 93.1 92.0 Average Infestation 56.2 25.7 Dusting Dates June 16 June 21* June 22* June 23 June 27 July 15 July 19 July 27 July 30 Aug. 3 Aug. 8 1928 Average Percent Infestation Un- Date dusted Dusted July 11 .60 1.00 July 17 3.70 2.20 July 20 2.20 .40 July 24 .50 .50 July 27 .70 .50 July 31 .25 4.00 Aug. 3 .75 3.25 Aug. 7 1.25 3.00 Aug. 10 2.80 10.50 Aug. 14 3.30 6.70 Aug. 18 1.71 3.10 Aug. 20 4.00 1.70 Aug. 27 22.60 2.40 Aug. 31 64.30 4.40 Sept. 7 70.00 20.60 Average Infestation 11.90 4.28 Dusting Dates Aug. 11 Aug. 1 6 * Aug. 18 Average Infestation 14.9 6.1 - _ I I --JI -V on the undusted plots was 36 per cent. Applications of dust were made July 15 and 19, which resulted in the infestation dropping to 8 per cent by July 26. Meanwhile the infestation on the un- dusted plots had advanced to 74 per cent. Applications of dust were made July 27 and 30, and August 3. This series of dust- ings kept the infestation below 20 per cent until August 2, and protected the newly-formed squares and young bolls. The in- festation on the undusted plots gradually advanced to 89 per cent, during the same period. An enormous number of adult weevils spread into the dusted plots practically equalizing the infestation on the dusted and undusted plots by August 6. The eleventh and last dusting was made August 8, for the purpose of protecting the half-grown bolls. The per cent of infestation, however, was not materially changed due to the reduced rate of squaring and to the migration of weevils from the undusted plots. The infestationi was complete (above 75 per cent) during the re- mainder of the season. The infestation count on the dusted plots from June 20 to August 2 indicated that the infestation had been sufficiently reduced (kept below 20 per cent) to protect a large number of squares and young bolls. The infestation in 1928 did not reach 10 per cent until Au- gust 10 (Table 1). Three applications of dust were made at intervals of two to five days. These dustings kept the infesta- tion below 20 per cent until September 7. The infestation on the undusted plots advanced from 2.8 to 70 per cent over the same twenty-eight-day period. Yields The contrast in seed cotton yields for 1926 (Table 2) on the dusted and undusted plots, show the effect of controlling the boll weevil when the infestation goes above 10 per cent. The gain from dusting varied from 190 pounds of seed cotton, where there was a 500-pound treatment of fertilizer, to a gain of 480 pounds of seed cotton per acre, where there was a 2000-pound treat- ment of fertilizer. The average gain for dusting on all fertilized plots was 359 pounds of seed cotton per acre. The greatest contrast in favor of dusting, however, was ob- tained in the 1927 season (Fig. 2). This contrast was due, main- ly, to a high infestation in early June which continued through- out the season, and the control obtained from dusting. There was a conspicuous contrast between the dusted and undusted plots. Where no fertilizer was used the gain was 224 pounds of seed cotton on the dusted plots. The gain from dusting on the plots treated with different rates of fertilizer varied from 562 pounds of seed cotton, where the fertilizer treatment was 500 pounds, to a gain of 931 pounds of seed cotton per acre, where the fertilizer treatment was 2000 pounds. The average gain per acre from dusting on all fertilized plots for 1927 was 773 pounds of seed cotton (Table 2). The contrast for dusting in 1928 varied from 142 pounds of Table 2.-Five-Year Average Infestation and Seed Cotton Yield Per Acre on Norfolk Sandy Loam Plots, Auburn, Alabam 1924-1928 Fertilizer Average Percent Infestation Seed Cotton Yield in Pounds Per Acre Per Acre. Treatment Ave. Ave. Ave. Lbs. 1924 i 1925 1926 1927 1928 { Inf. 1924 1925 1926 1927 1928 Yield Gai 0 Undusted 0 7 13 { 40 4 9 1071285 1481 741 681136.0 Dusted 01 1 61251 2 7 l1l21118291 311342 500 Undusted 35 1 7 17 { 60 11 26 45615091{484128212561397.51 Dusted 20 1 8 271 2 1 12 678 535 6741844 4511636.41239 1000 Undusted 38 I 9 I 13 59 1 11 I 26 7581 6951 8881 4731 5701 667.41{Dse 518 2 2 1 5 7 1 4 2 2 1 6 0 80 3 1500 Undusted 371 21I 13 { 57I 13I 28 1146I 7701 1212I 6061 710I 888.8I Dusted 23 I4 I5I 26 I5 13 1352 81311622114681 10501 1261.01 3724 {2000 Undusted 37 1 20 I 13 I 58 I 15 I 30 1304I 899I 1374I 565 I 1165I 1061.6 {se 875 2 5 1 0187 1 5 4 6 1 0 3 94 2 seed cotton per acre, where the amount of fertilizer was 2000 pounds, to a gain of 494 pounds of seed cotton per acre, where the rate of fertilizer was 1000 pounds (Table 2). The average gain per acre for dusting on all fertilized plots was 292 pounds of seed cotton. CECIL CLAY PLOTS, 1926-1928 T HE CECIL clay plots were located on the E. H. James farm, five miles west of Loachapoka, in Lee County. In 1926 the soil was treated with 450 pounds of home-mixed fertilizer per acre, in 1927 with 400 pounds, and in 1928 with 600 pounds. Infestation and Dusting The infestation in 1926 approached 10 per cent July 26 (Table 3). An application of dust was made July 27. There was a heavy rainfall within twenty-four hours. In fact there was a heavy daily rainfall for eight consecutive days from July 27 to August 3 making it impossible to apply further dust until August 5. Three applications of dust were then made at four- day intervals. The infestation on the dusted plots remained be- low 10 per cent until August 26, when it advanced to 23 per cent. On the undusted plots, the infestation gradually advanced from 13 to 50 per cent, during the same period. The infestation on the dusted plots in 1927 was 40 per cent June 14 (Table 3). The first dust was applied June 14, but was washed off within an hour. Five dustings were made from June 14 to 23 inclusive, all of which were affected by rain with- in twenty-four hours. The infestation on the dusted plots, how- ever, decreased from 40 per cent June 14, to 11 per cent July 5, while on the undusted plots the infestation advanced from 29 to 46 per cent. The dustings on June 25, July 6, 11, and 28, and August 2, and 5, were not affected by rains. The infestation remained below 20 per cent on the dusted plots from June 20 to August 5, except July 29, when the infestation was 42 per cent. The infestation on the undusted plots gradually advanced to 87 per cent during the same period. The infestation advanced to 80 per cent on the dusted plot and 96 per cent on the undusted plot by August 10. All applications of dust were made during the day. The condition of the foliage at the time of dusting varied from wet to dry. There was no appreciable difference in the control under either condition. The infestation in 1928 was above 10 per cent June 27 (Table 3). The cotton was dusted the same day and at intervals of four to six days until three applications of dust were made. The infestation advanced to 14 per cent July 30, and two applica- tions of dust, July 31 and August 4, kept the infestation below 20 per cent until August 25. Table 3.--Infestation and Dusting Dates on Cecil Clay Plots, Lee County, 1926, 1927, and 1928. 1926 1927 1928 Average Percent Infestation Average Percent Infestation Average Percent Infestation Dusting Dusting _Dusting Un- Dates Un- DatesUn-Dates Date dusted Dusted Date dusted Dusted Date dusted Dusted June 15 0.0 0.0 June 14 29.0 40.0 June 14* June 22 8.0 9.3 June 24 .2 .5 June 20 31.0 7.0 June 17* June 27 14.0 20.0 June 27 June 28 1.6 1.0 June 25 44.5 8.0 June 18* July 3 38.0 17.0 July 3 July 3 .8 .3 June 29 32.5 5.5 June 21* July 7 7.0 4.0 July 7 July 10 2.0 1.5 July 5 46.5 11.5 June 23* July 14 4.5 2.0 July 9 July 16 .8 .6 July 9 49.5 16.5 June 25 July 18 8.0 1.3 July 21 5.6 4.7 July 15 52.0 13.0 July 6 July 21 13.5 1.0 July 26 9.6 8.7 July 21 54.0 10.0 July 11 July 27 11.0 10.6 July 31 13.3 14.5 July 27 July 29 52.0 42.0 July 28 July 30 10.0 14.6 July 31 Aug. 6 13.2 6.1 Aug. 5 Aug. 2 74.0 19.0 Aug. 2 Aug. 3 9.5 16.0 Aug. 4 Aug. 10 8.2 4.3 Aug. 9 Aug. 5 87.0 25.0 Aug. 5 Aug. 7 12.0 8.3 Aug. 14 12.4 2.6 Aug. 13 Aug. 10 96.0 80.0 Aug. 11 13.0 1.0 Aug. 19 26.5 4.0 Aug. 17 Aug. 16 16.0 3.6 Aug. 26 50.4 23.3 Aug. 20 22.0 13.0 Aug. 25 38.0 28.0 Aug. 28 44.0 20.0 Average Average Average Infestation 11.27 5.7 Infestation 53.8 23.1 Infestation 16.8 9.9 *Rained within 24 hours. 10 Yields A definite correlation between seed cotton yield and infesta- tion occurred in 1926, 1927, and 1928 (Table 4). The infesta- tion was low in 1926, and the increase in yield of 150 pounds of seed cotton per acre on the dusted plot was correspondingly low; while the infestation in 1927 was high, and the increase in yield of 597 pounds of seed cotton per acre was correspondingly high. The correlation was not quite so pronounced in 1928. The average infestation was low when contrasted with the 490 pounds per acre increase in seed cotton yield on the dusted plot. A careful study of Table 3, however, reveals the fact that the infestation was high several times during the period squares were being set. The large increase in yield resulted, even though the average infestation for the year was comparatively low (Table 4). Table 4.-Five-Year Average Infestation and Seed Cotton Yield Per Acre on Cecil Clay Plots, Lee County, 1924-1928. Average Infestation Seed Cotton Per Acre Year Undusted Dusted Undusted Dusted Gain 1924 12 6 755 670 -85 1925 37 14 480 740 1 260 1926 11 5 1200 1350 I 150 1927 53 23 535 1132 I 597 1928 17 10 765 1255 490 Five Year Average 26 11 747 1029 L 282 HOUSTON CLAY PLOTS, 1926-1928 THE BOLL WEEVIL control on Houston clay was continued on the Joe Phillips farm, three miles east of Pike Road, in Montgomery County. The soil, which had been under cultiva- tion for twenty years, was not treated with fertilizer in 1926, 1927, or 1928. Different parts of the same field were used for the tests during the three-year period. Infestation and Dusting The infestation in 1926 was 11 per cent June 25 (Table 5). Three applications of dust were made at intervals of three to five days. The infestation dropped from 11 to 4 per cent, while the infestation on the undusted plots dropped from 8 to 4 per cent and then advanced to 14 per cent for the same period. A five-day period of rainfall, following July 26, prevented the further application of calcium arsenate at a time when it was needed to protect the newly-formed squares and young bolls. Three applications of dust, beginning August 11, reduced the in- Table 5.-Infestation and Dusting Dates on Houston Clay Plots, Montgomery County, 1926, 1927, and 1928. 1926 Average Percent Infestation Date June 25 June 29 July 2 July 9 July 15 July 19 July 23 Aug. 3 Aug. 9 Aug. 13 Aug. 18 Average Infestation Un- dusted 8.8 4.8 9.0 14.0 20.0' 24.0 29.0 31.3 42.2 50.5 43.3 25.17 Dusted 11.0 4.6 4.7 4.0 12.6 16.0 12.6 10.0 30.0 42.8 29.6 16.17 *Rained within 24 hours. Dusting Dates June 25 June 30 July 3 July 16 July 20 July 2 6 Aug. 11* Aug. 13* Aug. 17 1927 Average Percent Infestation Date June 4 June 10 June 15 June 22 June 27 July 2 July 8 July 16 July 22 July 26 July 30 Aug. 4 Average Infestation Un- dusted 8.0 4.0 7.0 6.0 12.0 34.5 28.5 28.5 68.5 76.0 72.0 88.0 36.08 Dusted 19.0 11.0 .25 2.0 14.0 44.8 11.25 8.0 61.5 70.0 57.5 72.5 30.73 Dusting Dates June 6: June 11 June 30 July 4 July 11 July 26 1928 Average Percent Infestation Date June 19 June 21 June 26 June 30 July 4 July 12 July 19 July 26 July 31 Aug. 4 Aug. 8 Aug. 17 Aug. 21 Aug. 25 Sept. 1 Average Infestation Un- dusted 4.0 5.3 2.6 5.3 1.3 6.6 23.0 30.0 31.8 26.3 50.0 49.0 55.3 24.0 Dusted 16.0 18.3 6.0 2.6 0.0 0.6 3.0 7.3 15.0 15.6 6.0 26.0 40.0 48.0 46.0 16.7 Dusting Dates June 21 June 30 July 4 July 31 Aug. 4 Aug. 24 Aug. '27 Aug. 29 i 12 festation from 42 to 29 per cent by August 18. All applications of dust were made in the day time, while the foliage was dry. The infestation in 1927 was 19 per cent on the dusted plot June 4 (Table 5). Two applications of dust were made at five- day intervals beginning June 5, which was nineteen days earlier than the first dusting in 1926. The third application of dust was not made June 16, on account of the infestation dropping to .2 per cent. A large number of adult weevils, however, had emerg- ed by June 27, and the infestation had advanced to 14 per cent. An application of dust was made June 30. The infestation had advanced to 44 per cent on the dusted plots July 2, which was a gain of 30 per cent infestation in five days. The applications of dust June 30, July 4, and July 11, reduced the infestation to 8 per cent on the dusted plot, while the infestation on the undust- ed plot advanced from 12 to 28 per cent. The infestation July 26 was practically the same on all plots due to weevil migration. All applications of calcium arsenate were made in the day time, with the foliage dry. The infestation in 1928 was 16 per cent on the dusted plot June 19 (Table 5). Three applications of dust at intervals of four to nine days kept the infestation below 15 per cent until July 31. Two dustings, July 31 and August 4, and three dustings August 24, 27, and 29, protected the cotton. Weather conditions pre- vented the application of dust August 17 and 21 at which times the infestation was above 20 per cent. Yields The increase in yield from dusting was less in both 1926 and 1927 than in 1928, although the infestation was as great or greater in each of the former years (Table 6). This condition was brought about largely by a failure to make one or two need- ed applications of dust at crucial periods in the development of the cotton in 1926 and 1927. The type of soil was such that the excessive rainfall which occurred during these periods prevented the operation of the dusting machine. The increase in yield from dusting in 1926, 1927, and 1928 was respectively, 170, 160, and 372 pounds of seed cotton per acre. WHEN TO DUST AND WHEN NOT TO DUST COTTON AS SHOWN BY THE INFESTATION IN 1926 AND 1927 HE question of when to dust and when not to dust cotton to control the boll weevil profitably is quite clearly shown by the infestation and yield records of 1926 and 1927, on the rate of fertilizer plots (Tables 1 and 2). The late and low infestation during the 1926 growing season is in striking contrast to the ear- ly and high infestation in 1927, which continued throughout the growing season. The infestation in 1926 did not reach 10 per cent until August 27. Three applications of dust were made which resulted in keep- 13 Table 6.-Five-Year Average Infestation and Seed Cotton Yield Per Acre on Houston Clay Plots, Montgomery County, 1924-1928. Average Infestation Seed Cotton Per Acre Year Undusted Dusted Undusted Dusted Gain 1924 30 12 235 405 170 1925 22 13 670 1090 420 1926 25 16 690 860 170 1927 36 30 657 817 160 I I 1928 24 16 388 760 372 Five Year Average 27 I 17 528 786 258 ing the infestation below 20 per cent until September 14. The infestation in 1927, however, was 11 per cent June 13 (Table 1). Three series of dustings were necessary throughout the season, the first beginning June 16 and extending to June 27. Three applications of dust were made at intervals of four and five days. Rain, June 21 and 22, necessitated the repetition of dustings. The second series began July 15, when the infestation reached 15 per cent. Following the second application of dust, July 19, the infestation was reduced to such an extent that further dust- ings were unnecessary until July 27. The third series was ap- plied July 27, 30, and August 3, respectively. The infestation was complete (above 75 per cent) on all plots by August 6. These data show that the infestation determines the time to begin dusting and whether or not dusting is necessary. These data also show that no reliable date can be set on which dust should be applied. FIVE-YEAR AVERAGE YIELD OF SEED COTTON PER ACRE ON THREE SOIL TYPES THE five-year average gain of seed cotton per acre from dust- ing with calcium arsenate for boll weevil control was quite uniform on the three soil types. The comparable plots had been treated with approximately 500 pounds of fertilizer per acre, ex- cept that on the Houston clay, no fertilizer was used in 1926, 1927, and 1928. The approximate seed cotton yield per acre on the dusted plots of the three soil types was as follows: Norfolk sandy loam, 600 pounds; Houston clay, 800 pounds; and Cecil clay, 1000 pounds (Fig. 1). The range of gain or loss from dusting cotton on each soil type was quite variable throughout the five-year period. On the Norfolk sandy loam plots the range was from 74 pounds of seed cotton in 1926 to 562 pounds gain in 1927 (Table 2), with a five year average gain of 239 pounds of seed cotton per acre (Fig. 1). The greatest range of gain or loss from dusting cotton for the 'JIt iUhdu I, d 565 P,,wid Seed C5 I' , I'ud_ Seed Cotton Cotton Fig. 2.-Cotton on Norfolk Sandy Loam soil treated with 2000 pounds of fertilizer per acre. 1927. control of 1)o11 w'O\ il w as on the (e(t(if clay llots, the range vary- ing from 85 p)ound loss oni the dulsted lI)ots in 1921 to a 597 po0011d gain in 1927 (Tfable 4)with a fiv e-year average g(ain of 282 p~oundls of seedl Cotton pci acre (Fig. 1). The range of gain on the 1Ikuston clay- plots Nv as from 16(0 poundls in 1927 to 420J iofutls 0-