November 1986 Agronomy and Soils Departmental Series No. 112 Alabama Agricultural Experiment Station Auburn University Lowell T. Frobish, Director Auburn University, Alabama T ,&sa4 12 c p cp)r t O o -5 1986 REGIONAL COTTON FUSARIUM WILT REPORT 1 W. C. Johnson Cotton cultivars and elite breeding lines submitted by 20 cooperators were evaluated for fusarium wilt resistance under field conditions at the Plant Breeding Unit, Tallassee, Alabama. These entries were grown on a Wickham sandy loam highly infested with both the fusarium wilt fungus (Fusarium oxysporum Schlect. f. vasinfectum [Atk.] Snyd. & Hans.) and root-knot nematodes (Meloidogyne spp.). Plots were 40-inch-wide bedded rows, 30 feet in length, separated by 6-foot alleys. Four replications of the test entries and checks, arranged in a block design, were evaluated. Both susceptible (Rowden) and resistant (McNair 235) cultivars were included as checks. Rowden was planted in row 5 and every tenth row thereafter (15, 25,..., 215) and McNair 235 in row 10 and every tenth row thereafter (20, 30,..., 220) throughout the test. Plots were planted May 5 and thinned to three or four plants per foot on June 20. Initial live plant counts were also made on that date. Wilted plants were counted, removed, and recorded on July 18, August 8, August 20, and September 12. The remaining live plants were also counted and recorded on September 12. 1This report is a joint contribution between USDA-ARS, Crop Science Research Laboratory, Mississippi State, Mississippi, and the Alabama Agricultural Experiment Station, Auburn University, Alabama. Professor of Agronomy and Soils. -3- Percent wilted plants per plot were then determined and mean wilting for a given entry calculated. The incidence of wilt was high within the experimental area and was not excessively variable, appearing to be more or less randomly distributed. Average wilting of the susceptible Rowden was 79.2, 81.3, 80.5, and 82.6 percent for each of the four replications. The mean incidence of wilting in the resistant check, McNair 235, was 21.3, 26.9, 16.8, and 20.5 percent in the corresponding replications. The long term average for McNair 235 is 12 percent wilting. Critical evaluation of a given entry should be made relative to the checks closest to the entry within each replication. Evaluation of breeding progress or evaluation of entries over years should be made only between the relative value of this entry and that of the closest susceptible check rows for each year. Entries submitted by W.C. Johnson are commonly grown cultivars or advanced commercial materials. Thus, these entries are listed by name. Entries submitted by other cooperators are listed by their coded numbers. Additional information regarding the genetic background of a specific coded entry should be obtained from the named cooperator. ACKNOWLEDGMENT The author express appreciation to A.J. Kappelman, Jr., retired, for advice and technical assistance in conducting the test and preparing this report. -4- 1986 Regional Cotton Fusarium Wilt Test, Plant Breeding Unit, Tallassee, Alabama Test entry Percent wilt by replication designation 1 P . M. Thaxton, Dept. of Plant Path., Texas A&M, College Station, TX 77843-2132 PMT-1............ * 48 29 41 76 48.5 PMT-2 .76 70 18 57 55.2 PMT-3 ......... 36 11 56 42 36.2 PMT-4 -4.........63 84 90 96. 83.2 Rowden ............ 85 90 96 87 89.5 PMT-5 .. ....... 16 14 10 21 15.2 PMT6.......38 1 12 24 18.8 PMT-7 .' . . . 54 14 47 44 39.8 PMT-8 . .......... 33 26 45 26 32.5 McNair235 .. ... .. 8 6 55 42 27.8 Lynn McDonald, Dir. o~f Research, Stoneville Pedigreed Seed Co., P.O. Box 167, Stoneville, MS 387761 08456897 0 2 . . . . . . .86 74 41 56' 64.2. 3.........60 38 33 71 50.5 465 64 81 84 73.5 Rowden . . . 85 88 97 78 87.0 5C44 12 33 58 36.8 634 39 50 34 39.2 7........ 32 81 59 82 63.5 8...... 52 63 48 65 57.0 McNair 235 .... ... 55 55 24 20 38.5 -5- 1986 Regional Cotton' Fusarium Wilt Test., Plant Breeding Unit, Tall assee, Alabama Test entry Percent wilt by replication Mean designation 1 2 3 David Bush, Rogers Delinted Cotton Seed Co., P.O. Drawer 1340, Waco, TX 76703 DLB 1 96 41 77 61 68.8 DLB 2 86 12 68 71 59.2 DLB 389 51 96 83 79.8 DLB 4 57 41 /1 35 51.0 Rowden . ....... 65 92 100 74 82.8 DB5.. .. ... .. 35 56 79 58 57.0 DLB . ........... 39 30 85 32 46.5 DLB 7 .. .... . 19 7 21 12 14.8 DLB 8 ..... . .33 81 84 54 63.0 McNair 235. ...... .16 16 30 19 20.2 Gene Douglas, Hollandale Agricultural Ser., P.O. Box 397, Hollandale, MS 38748 1611 20 19 8 1200 1602 . .... ...... 12 22 10 9 13.2 163>53 58 56 7 43.5 1644 2 17. 13 9.0 Rowden.. .... . 72 99 92 84 86.8 1659 9 22 33 18.2 10 ..42 71 25 50 47.0 1607. ......... 38 17 56 43 38.5 1608..... . 64 50 60 33 51.8 McNair23 14 18 54 2 22.0 -6- 1986 Regional Cotton Fusarium Wilt Test, Plant Breeding Unit, Tallassee, Alabama Test entry Percent wilt by replication designation 1 2 3 4 R.R. Bridge, Delta Branch Exp. Sta., P.O. Box 197, Stoneville, MS 38776 RR1.53 36 10 24 30.8 RRB-2 .......... 33 1 18 11 15.8 RRB-3 .......... 86 5 14 14 29.8 RRB-4... 62 66 34 75 59.2 Rowden ............ 66 96 72 82 79.0 RRB-5 ..... 3 2 23 21 12.2 RR. 23 11 25 30 22.2 RR. 73 57 56 37 55.8 RRB-8 . .. .48 76 33 65 55.5 McNair 235 . .23 40 3 14 20.0 John Green, 101 Sycamore St., Leland, MS 38756 NK-1............. 59 48 11 41 39.8 NK-2......... .. 17 14 15 36 20.5 NK-3 ... 29 66 47 77 54.8 NK411 24 9 5 12. 2 Rowden . w d e n . . 89 .100 92 83 91.0 NK5............. 21 11 19 10 15.2 N. 18 24 13 8 15.8 N -7.44 12 18 7 20.2 NKB10 0 11 10 7.98 McNair2 ..... 52 15 10 34 27.8 Kamal M. El-Zik, Dept. of Plant Path., Texas A&M, College Station, TX 77843-2132 KME-1....... . . . .24 69 3 36 3300 KME-2 ....... .... 39 39 25 56 39.8 KE332 39 6 53 32.5 KME-4.........35 20 4 7 16.*5 Rowdepn .. ......... 90 86 30 97 75.8 -7- 1986 Regional Cotton Fusarium Wilt Test, Plant Breeding Unit, Tallassee, Alabama Test entry Percent wilt by replication designation 1 2 3 4 Shelby M. Baker, Costal Plain Exp..Station, P.O. Box 748, Tifton, GA 31793--0748 GAT-1 . . . . 55 15 8 20 24.5 GAT-2...........69 26 0 33 32.0 GAT3. .. 54 32 2 32 30.0 GAT-4 ......... 40 31 3 23 24.2 Rowden .w d e n . . . 89 45 49 78 65.2 GAT-5 .. ... .... 32 54 33 29 37.0 GAT-6........ . 42 28 13 50 33.2 GAT-7... .. .. 36 31 8 32 26.8 GAT-8 . ..... 14 14 13 31 18.0 McNair 235......34 14 6 22 190 Jerry Rice, Cargill Seed Division, Box 1630, Plainview, TX 79072 1. .. .. ...... .... 20 23 3 45 22.8 2 . . . 0 * . 3 25 1 11 1000 3 ... . ..... .. 60 20 6 28. 28.5 4 . . . . . .. .13 11 6 42 17.2 Rowden ... ... 80 84 86 76 81.5 5 ........... 23 9 0 10 10.5 7 ........ .... . 18 100 0 23 35. 8 .. . . . . . . . . . . 23. 79 6 45 38.2 McNair 235. .... 24 83 5 35 36.8 J.B . Weaver Jr., Dept. of Agronomy, Un iv., of Georgia, Athens, GA 30602 1 . . . . . . . . . . . . . . . 35 27 3 48 28 .2 2 .> . . . . . . 53 23 13 13 .25.5 3 .. ... .... . . 24 8 6 60 24.5 4 .. ... .... .. .. . 42 65 17 72 49.0 Rowden ...... 72 100 77 82 82.8 5 . . .. . . . .. .. 0 13 1 5 4.8 6 . . . . . 1* . . 0 .0. 0 43 2 12 14.2 7 -- ---- -13 36 6 12 16.8 1986 Regional Cotton Fusarium Wilt Test, Plant Breeding Unit, Tallassee, Alabama Test entry Percent wilt by replication designation 1 2 3 4 Carl A. Moosberg, Gro Agri Seed Co., P.O. Box 1656, Lubbock, TX 79408 GA-1 33, 63 1 10 26.8 GA-2..... 47 58 58 54 54.2 GA-3 3 .27 14 0 22 15.8 GA4 7 27 4 22 15.0 Rowden ... ..... 55 86 71 97 77.2 GA-5 ........ .8 10 1 48 16.8 GA -6 . 0 .0 . . . . - - - 0 GA-7 ..... ... ...- - - 50 - GA-8......... . 11 0 7 0 4.5 McNair 235....21 85 3 1 27.5 Keith R. Jones, Delta & Pine Land Co., P.O. Box 157, Scott, MS 38772 1...... 6 61 0 5 180 2 ....... . 0 50 2 8 15.0 3 . . . . . 27 29 14 25 23.8 4......... 14 29 4 13 15.0 Rowden ........... 78 100 79 26 70.8 5 . . . . 5 42 2 98 36.8 6 .51 18 10. .53 33.0 7 .... .... . . . 72 59 21 37 47.2 8 . .. ...... 86 59 28 26 49.8 McNair 235 : ... 15 35 5 26 20.2 Gary L. Rea, Terra Seed Co., P.O. Box 10121, Lubbock, TX 79408 TC1..26 36 6 64 33.0 TSC-2. .............58 37 8 63 41.5 TC3.. .... 36 2 21 28 21.8 TSC-4 ..... 73 22 87 65 61.8 Rowden............100 81 87 97 91.2 TCS28 4 12 46 22.5 TC6.3 2 35 27 16.8 TS 73 2 4 3*2 10. 2 TC83 6 6 37 13.0 McIir 358 &5 178. -9- 1986 Regional Cotton Fusarium Wilt. Test, Plant Breeding Unit, Tal lassee, Alabama Test entry Percent wilt by replication designation 1 Fred Bourland, Dept. of Agronomy, P.O. Box 5248, Miss. State, MS 39762 FMB-1 . . . . 36 34 5 62 34.2 FMB-2 19 5 5 30 14.8 FMB-3 . . . 25 20 13 15 18.2 FMB-4 . .. 32 6 2 20 15.0 Rowden.......... 100 67 75 100 85.5 FMB-5....... 27 14 22 23 21.5 FMB-6...... .... 23 14 16 9 15.5 FMB-7 ..... .. . 21 37 10 75 35.8 FMB-8 . . 32 13 13 26 21.0 McNair 235 ......... 16 33 1 12 15.5 Laval M. Verhalen, Dept. of Agronomy, Okiahama State Univ., Stiliwater, OK 74078 OKLA-1........ 8 46 18 8 20.0 OKLA-2......... 8 10 29 17 16.0 OKLA-3 .. ... ........ 9 0 0 4 3.2 o . .. .4 35 11 14 16.0 Rowden 55 94 91 88 82.0 OKLA-5.........45 67 46 63 55.2 33 53 23 44 38.2 OKA7 .1 1 5 3 2.5 OKLA-8..... 63 73 69 66 67.8 McNair 235 2 20 14 '21 14.2 Mason, Hawkins, Ranger Seed Co.., Box 1288, Tahoka, TX 79373 RSC-1 . ....... 9 14 2 53 19.5 RSC-2........ 12 33 16 66 31.8 RSC-3........ 59 82 14 36 .47.8 RSC-4 ........... 29 58 20 37 36.0 Rowden ....... . . . 78 99 89 96 90.5 RSC-5 .... 1 15 27 21 35 24.5 RSC-6. 16 36 0 21 18.2 RSC-7.......... 54 13 5 56 32.0 RS- ..... 431 46 42 67 49.5. - 10- 1986 Regional Cotton Fusarium Wilt Test, Plant Breeding Unit, Tallassee, Alabama Test entry Percent wilt by replication M designation 1 2 3 4 Jack E. Jones, Dept. of Agronomy, LSU, Baton Rouge, LA 70803-2110 JJ-1 * ... 6 20 14 13 13.2 JJ-2 0 23 9 5 9.2 JJ-3.. 0 8 1 8 4.2 JJ-4 4..0 5 16 5 6.5 Rowden..... .. . 90 29 70 100 72.2 JJ-S . . 5 3 4 6 4.5 JJ -6 . . ... . ... 0 0- 8 50 14.5 JJ -7 .... .......... 31 5 54 44 33.5 JJ-8 . . . 8 0 33 16 14.2 McNair 235...........11 20 29 21 20.2 Henry W. Webb, Coker's Pedigreed Seed Go., Hartsville, SC 29505 1 . . . .. 42 8 47 52 37.2 2 ............. 14 16 48 37 28.8 3 . . . . . . . . . .. . . 18 0 25 77 30.0 4 . ......... 42 0 59 71 43.0 Rowden...........92 31 100 89 78.0 5..... . . . . . . 21 5 16 72 28.5 6.......... 16 18 31 45 27.5 7 . .... ... 12 5 10 42 17.2 8 ... ... . . . 6 36 26 56 31.0 McNair 235. ........ 18 1 11 35 16.2 Joel Mahill, Gro Agri. Seed Co., Lubbock, TX 79408 1.... . . 59 27 43 37 41.5 2 6..31 4 49 31 28.8 3....... . . . 36 5 55 21 29.2 4.19 6 62 39 31.5 Rowden . . ... . 71 94 95 47 76.8 5.. .. . 16 12 .76 76 45.0 6.... 30 12 24 13 19.8 7.......... . 50 10 22 27 27.2 8 . .. . .. .. . .. . 9 11 14 20 13.5 MNa ir 25.... 18 10 23 17 17.0 -r 11- 1986 Regional Cotton Fusarium Wilt Test, Plant Breeding Unit, Tallassee, Alabama Test entry Percent wilt by replication- Mean designation 1 3 W.C. Johnson, Dept. of Agronomy and Soils, Auburn University, AL 36849 Coker 80-118.......... 10 11 23 12 14.0 Deltapine 90 ......... 44 20 26 22 280 Stoneville e82 26 51 98 81 64.0 Delott344 8 4 23 15 12.5 Rowden 51 100 80 100 82.8 Tifcot 56. .. .... 50 66 57 40 53.2 DES 422........... 15 27 10 9 15.2 Coker 315.......... 30 72 38 44 460 McNaira22 26 39 19 30 28.5 McNair 235.19 16 45 1 20.P2 Coker 208 46 16 57 59 44.5 DES 11.. .. . . 21 39 19 19 24.5 UArk2402........ 35 28 74 38 43.8 Delcot 39. ...... 25 14 56 32 31.8 Rowden 91 82 91 56 80.0 Deltapine6l.58 55 46 70 57.2 Coker 13 e 32 8 68 49 39.2 GaT 81-225 26 23 18 52 29.8 Stoneville 112 19 24 35 23 25.2 McNair 235 18 20 17 23 19.5 Stoneville 506 ........ 3 53 18 23 24.2 Deltapine 50 14 34 52 67 41.8 Del tapinee2027 .30 54 29 35.0 PD1........... 24 36 53 87 50.0 Rowden. .. 88 46 51 100 71.*2 Delcot 311...........11 1 16 17 11.2 Stoneville l3 57 27 52 80 54.0 Deltapine 4 ...... 49 7 38 24 29.*5 Coker 81-613...... 28 38 19 28 28.2 McNair 235...........13 12. 5 34 16.0 -12-