'Vt- ;K>t ~ 4 .A ,t-W t .n _I V . - -".X1 '4 ff4 ti. \\ S i YY , *q ~ ' 7 YkC'. t_ A .' E .. '+ 7 ft .! . y y_ We L V R .L..y , 1711 Pb 44- ".n .s DIRECTOR'S COMMENTS nematodes, and pathogens has been a major problem confronting agricultural and forestry production for decades. Earle control of crop pests was limited to cultural practices such as crop rotations. With the de velopment of the science of plant breeding, many new ctltivars were developed that were resistant to pests. These new varieties, coupled with crop rotation practices, sustained agri cultural production for a short time. In the 1940's, the discovery of organic com- LOWELL T. FROBISH pounds such as DDT, 2,4-D, and BHC (benzene hexachloride) heralded a nes era in the management of pests. Yields of many crops increased significantly through the 1960's as pesticide use grew rapid ly. Concerns mounted as the potential for detrimental environmental effects and human health risks was recognized, and safer and less persistent pesticides were developed. Because of agriculturists' concern about the heavy use of synthetic chemicals, research was initiated to control pests through a variety of methods - called a systems approach. Integrated pest management (IMP) is one such approach. It is an ecologically based pest control strategy that relies on natural mortality factors (natural enemies, weather, crop management, etc.). 1PM involves all aspects of crop production, such as cultivation, fertilization, postharvest management, tillage practices, selection of pesticides, and tim- ing of applications. Use of more specific, less biologically active pesticides is a major principle guiding 1PM development. Genetic resistance is still the most important defense mechanism against pests. The use of recombinant DNA techniques, gene transfer, and cell culture, coupled with traditional plant breeding, will result in the develop- ient of cultixars that are both resistant to pests and tolerant if synthetic agricultural chemicals. The application of biological agents to control pests is a ne technology of much promise. Biocontrol may mean utilizing natural microorganisms to control weeds, weeds to control insects, or insects to control weeds and iicroorganisms. Microorganisms may be used to control other microorganisms, a plant's own defense system may be enhanced by inocula- tion with an axirulent pathogen, and iaturally occurring cheiicals or wseed diseases may be used to control diseases. All of these offer an alternative to the use of synthetic chemicals, but they will not eliminate the total need for pesticide use. SPRN(, 1990 VO1. 37. NO. I A quarterly report of research published by the Alabama Agricultural Experiment Station, Auburn Universits IOWELL T. FROBISH. .. Director )AVID H. TI I.. .\ssociate Director DAILE L HUIFFMAN .Interim Associate Director C HARtLES W. BRJCE.. Assistant Director R.E. STEVENSON...............Editor ROY ROBERSON ........ Associate Editor KA\TIE SIT H..........Assistant Editor TERESA RODRIGUEZ...... Art Designer Editorial (ommittee: ILowell T. Frobish; L t. Lamn k, A."ociate Procssor of loilir and Child Dci elopm0nt: N.WV. Rochester, As sociate Professor o1 Agrictltural 1 ngi- ,tering; S. P. Seuiiidt, Associale Professor of Animal and I)airi Sciences; I). I. Bransbs, A ssociate P'rolessor of A eroniooi v and Soils; N.R. Holler, A ssociate Irofessor of /ool- oK' and WIilllife Science, .l. 1). et, Pro- fesesor of Bolan ' and Microbiologj; 1'.A. Duff x , A s.istsant Profec'sor 01 1i'AriclturaI Eotnomis an(d Rural Sociolog ;, R.I Iox ell, Prcs iccsc r of Fiscerices and A/lied 4qwacultrcs; I.P. Mack .A ssociate Pro essor of Entomologj'; and R. F. Stex enson. MAY WE INTRODUCE Dr. Glenn Wehtje, Associate Professor of Agronomy and Soils. A native of Washington State, Wehtje earned a B.S degree in agricultuii from Washingto. State University, an M.S. in plant pathology froni North )akota State Unixersity, and a Ph.D1) in agtonomx and wxeed science fron the U.niver sity of Nebraska. Wehtie came to Auburn in 1981 as an Assistant Professor and was promoted to Associate Professor in 1986. Since Comine to Auburnii, Wehtje has worked extensixely xx ith peanitt wxeed control. His research teamti xas instrumental in developing timing and rates of paraquat for use on peanuts. Para quat has sibsequently become the leading replacement for dinoseb-containing her- bicides that xwere banned in 1987. In addition to his herbicide screenine work, Wehtije also works etensively in the area of herbicide movemeni in the soil and herbicide interaction xith other cropping practices. His report on xork xxith different peanut seed sites is featured on page -. ON THE COVER. Live oak trees in large con- tainers grew faster when water was kept at a constant level in saucers. See story on page 11. EDITOR'S NOTE. Mention of trade names does not indicate endorsement bx the Alabama Agricuntural Experiment Station or Auburn Uiversits of one branid over aniot her. Any use of pesticide rates in excess of labeled amounts in research re- ported does iot constitute iecommenda- tion of such tate. Such use is simplx part of the scientiic investigation necessary to evaluate various materials. No chetical should be used at rates aboe those per mitted by tle label. Information con- tained herein is axailable to all persons xxithout regard to race, color. sex, or na- tional origii. K.S. RAO and D.A. ROLAND. SR. UNDERSTANDING CALCIUM SOLUBILIZATION IN HENS MAY BENEFIT EGG PRODUCERS -. \I ACIN Hits uita for egg shell C ot matiron, but it mnust be dis- sols ed in the digestixe Csystem (calcium solubilization) ot liens betfore it is ax aitable tot absorp- tion. 13x u nderst andi ng hoxw calciumiii solubil ization occurs and hoss it is at- fected by exter nal factors, egg pi oducers can miake more e t teetit e decisions regardcitri the pr oper fotrmt arid quanti- tot calcium su pplement needed. \tfter incestiott, calcium supplemniits limestone o) oxyster shell) react wiith the digtestive acids secreted in the proxen- Ircutus (glandular stomach) of hens and presenti in the gizzard (mnuscular st omach), to release cateiumn in a tormn ax ailable tot absorptiton ( bioax ailable calcium). Inlitial expeimerits at the Alabama Agictirral E xperimnent Sta- tion (.AAES) showsed that 701o of the 3.*75 g (grains) ot the darts calcium con- sumed wxas sotubilized in the dicest ix sxxtern of helix. A\dditional \AAES e'xperiments in- dicated that, on a percentage basis, calcium solubilization and uitilizatiron (calcium consumed mninus calcium cx- cireted) deer eased ax calciutm consump- tion bx hens tincr eased, see table. HIoxs cxer, the abxol ut e qciant ityr of calciutm sotubi lizedt and uttilizedt by hens netreased x ith the amount 01' calcium cons trued. I he particle size of the calciurn sup- plerment also intluenced calcium solubilization . As shown r by data in thle table, liens sol ubilized a greater per cen- age o I calciut t rom tatk gerisus small- particle limnexione at eacti ot the dietaryx lx cls tested. L arge par ticles of the calcium supplement xxerc held in the giz- .'ard tot a latiget period oit t ime, cans- ing a ciraduial sot cbi lizat ion that alloxwed continuous intestinal absorption. I heretfore, during the night xwhen calciutim needs f or egg shell calcification ateci gratest , latrcc particles held in the cizzar d courld still suipplx calcium to the cdigextix e sx sterm. A prolonged suippix of sotlubiized calciurm to the intest ine of liens ted lat ge-particle limestone allosxed hese birds to ti lize more calcium than hens fed small-patrticle limestone, see able. Sma~l Ipatrticles 01' calciumn stipple- men t, on thle other hand, are an immer1diate soutrce of calcium to actixve- xI fectiric laxting liens. Although smtall- particle calciurn supplement is htighly soluble, its r esidernce tirme in the gizzard is shior tci arid thtiti its oxverall soluibilits' is decteasedt. [tens recluire Cappiroximnately 2 g of calciutm daily to totm inr egg shell; based on the AAL S stitch, calcium in the diet needed to meet t his r eqtitremernt could be toxwerect by feedirng only~ large- instead ot small-patrticle limestonme. Hoss cxc, in tie commrntciat taxer industry, using an excluisix cly large-particle calcium stipple- mnent miax inrttease calcium separation in he teed, wxastage of calcium stupplemtent bx hens, arid expcense. The exper imnt also supports the pcopular pr act ice of addinrg a port ion of tie calciumi supplemet iii taxer leeds iii flie totm iii1' pullet- or hen-sized partitelex (timiest one or oxxt ci shlf ), arid t he test init he f orm in f puts erizect limecstocnie. Partial iniccorpotratio torici one-third to tine-hatf Icf a tar ge-particle calcium xc) crce in thc dict should inetrease calciumn hioavailabilitv during adverse situnat ionx, such as elex atecd en iron- merita atmiiper atutres arid inradequcate feceder space. It should bc rioted thtat largc-particle calcium i stipplemttiis cannot be u sect iii scomie situtions. Somei t'eettirta xxysterms dco tot operate e cfticient l it larce partitelex ate used. Pellet feceding is practicedt in sonie birceder operations L arge-particle Iimenei' a( o u m d .1 ,AISiH~ . . .41) 2.91 4.00) 4.8t ).30 c(a wuthicJ., 1.43 2.6 t 1 3.5 2.63 C.a uuiuiuceJ pct . .... 96 8S tit 80 87 Ca iutih/ed, g .. . . . . . . . .6 t.9) 2.4)) 2.S4 2.0ii ( a utiied, pct . . ..i. . . . 7 2 64 S6 68 Small-particle hmreslmWi Ca con uI Wile gt . . t... 145 2.90 4.3)2 Sa.4 t .52 ( a xIltIhiti/ed . .. t. 1.3) 2 23 2.76 2.9' 2.33 C a sotihicire. pct. 1 81 6) 54 72 ( a tii/e , .. .. ... . ... 1. 2 1.6)) 2.07 2. '2 1.75 ( a uitiicd, .5 . ... 48 41t S ii 5..e-pa(hic III.imcItc 2 to 4 nun (panicle, pa~xd thI outI US.A. '.,xt daII et ig .5111 #le , bu we'. I)IIx ta I ined he moee 1110). smallar)taiic lt c~,iVInx O(I to 0.8 nun1 (pas cxl through sixx #20, but crx. IciIIIx hx mcxe n30). to) pre~II inte ro pagat~in of bacteri ia, and large parti- cles in the feed tend to reduce pellet- binding capabilities. To ensure stlticient ealcitum sciltiiliza- tioni ini these situ~a- ticons, higlier lexvels of dlietars' calcium in the formn of small- particle limestone cart be cised. Rao is G(auatne Re -'aiC1 6 \ssta andx Roland is Prtcixl smV o Pot l)I v' SICflcc. "l /u/)U/h I gi-icuituiral EI x)e/'im)ent Station G.R. WEHTJE, T.V. HICKS, and J.P. BOSTICK PEANUT YIELDS COMPARABLE FROM LARGE VERSUS SMA.l. SEED 4 ..- j# P EANUT GROWERS can save up to $30 per acre by using smaller, "high count" seed. Whether these smaller seed tolerate herbicide damage and other production rigors, and ultimately pro- duce comparable peanut yields to larger, more expensive seed, is not clear. To determine effect of seed size, three sizes of seed were planted in Alabama Agricultural Experiment Station tests at the Wiregrass Substation, Headland. Seed sizes included: small (1,515 seed per pound); medium (1,185 seed per pound); and large (825 seed per pound). The number of seed planted per acre was constant for the three sizes. Three weeks after emergence, plots within each of the ,O'l ! iX::u - seed sizes were treated with Gramoxone at 0.125 or 0.250 lb. ac- f' tive ingredient per acre. Some plots received two applica- tions of the lower rate of Gramoxone. Upon germination, the smaller seed pro- duced smaller plants, and the medium and large seed produced progressively larger plants. Herbicide ap- plication always tem- porarily stopped growth, regardless of seed size. Measure- ment of canopy width revealed that these in- itial differences re- mained throughout much of the growing season. The rate of growth appeared to be - similar, with the larger seed maintaining larger plants throughout most of the growing season. Top left shows ear Though Gramoxone peanuts from large had the expected effect peanuts had beer of temporarily stop- ping growth, it was ex- pected that the larger plants would recover , . more quickly. This did not appear to be the case. The smaller plants, from small seed, appeared to recover just as rapidly from any of the her- .:: ', bicide treatments. The direct relation- ship between increas- S, ing seed size and yield was evident, but not great. Small seed, averaged across the various herbicide treatments, yielded 3,180 lb. per acre. Medium seed yield- ed 3,280 lb. per acre and large seed yielded 3,250 lb. per acre. It must be pointed out that these data are from 1 year (1989), and this was a nearly perfect climatic growing season. Total rainfall at Headland for May, June, and July was 24 in. Timely rains nearer harvest time provided ideal full season growing conditions. It is expected that stresses from drought and other climatic factors will cause a bigger yield ly season peanuts from small seed, bottom left shows e seed, and above shows medium seed; none of these n treated with postemergence herbicides. differential among the seed sizes, but this is not known. Based on the 1-year test, it appears that the extra cost of the large seed simply represents a means to enter the growing season with relatively larger plants. The value of these larger plants, under different growing conditions, remains to be determined in future phases of the research. Wehtje is Associate Professor and Hicks is Research Associate of Agronomy and Soils; Bostick is Executive Secretary, Alabama Crop Im- provement Association. Alabama Agricultural Experiment Station - a J.D. NORTON, G.E. BOYHAN, J.E. BROWN, and M.H. HOLLINGSWORTH NEWLY LABELED HERBICIDE PROMISING FOR WATERMELON AND CANTALOUPE WEED CONTROL NCONTROLLED weeds can seriously limit melon produc- tion. Weeds are a problem during two critical periods of the growing season: (1) during seed germination when weeds grow rapidly and provide damaging competition to the crop, and (2) during the last 2-3 weeks of the production cycle, the time when the majority of sugars are pro- duced in melons and when weeds shade the melon plants to disrupt sugar forma- tion. The vining nature of the crop makes weed control difficult during most of the growing season. Effective and safe herbicides for use in watermelon and cantaloupe produc- tion have been sought for several years in research by the Alabama Agricultural Experiment Station. Unfortunately, only a few herbicides are cleared for use on watermelons and cantaloupes. Cur- bit has recently been labeled for this use, joining already labeled herbicides Prefar,? Alanap,? and Poast.? In previous years' tests at the North Alabama Horticulture Substation, Cullman, AmibenO at 2 lb. a.i. (active ingredient) per acre and a combination of 1 lb. Sonolan' and 2 lb. Amiben" gave best results. However, neither is labeled for use on watermelons and can- Coo I If GRASS ,ND BRDADI F WEEDS [ WAllRAILLONS A\D C ANFAIOPES, NORTH AIABI1A HOR H lR RE SLRSI AFION 1989 Grass and broadleaf control Treatment,,In wantaloupe In! atermeon, lb, a.i.acre at 33 days At 33 At 82 _ days days Pct. Pct. Pct. Prefar & Alanap, 3.0 and 1.0... 63 75 53 Curbit, 0.5.. 63 90 73 Curbit. 1.0.. .. 73 90 83 Curbit, 1.5..... 88 90 73 Command, 0.5 . 40 58 25 Command, 1.0. 60 73 28 Command, 1.5 . 75 78 33 W5eeds present in the experimental area included: grasses-craberass, goosegrass. carpeterass. and panicum: broadleaf weeds -pitgeed, lambsquarter, ragweed, purslane, and morningelors Viate melon trial termtnated before second rating could be completed. taloupes. Furthermore, Amiben severely damaged plants under certain weather conditions. Poast, the only post emergence herbicide labeled for melons, gave fair control of grasses but did not control broadleaf weeds. In continuing tests in 1989 at the same location, several different herbicide treatments were evaluated on both watermelons and cantaloupes. 'The standard treatment of Prefar + Alanap was compared with Curbit, and with another promising, but nonlabeled product, Commandic" Treatments were as follows: 1. Prefar - Alanap, 3 lb. and 1 lb., respectively, per acre. 2. Curbit, 0.5, 1.0, and 1.5 lb. per acre. 3. Command, 0.5, 1.0, and 1.5 lb. per acre. The herbicide treatments were spiay- applied immediately after AU-Producer watermelons and Chilton cantaloupes were planted. Watermelon test plots were single rows, 7.3 ft. wide and 60 ft. long, with hills spaced 6 ft. in the row. Cantaloupe plots were two rows, 3.7 ft. apart and 30 ft. long, with hills spaced 4 ft. apart. Plots that received no herbicidal treat- ment had a heavy infestation of both grasses and broadleaf weeds. Control from the herbicidal treatments was com- pared with the untreated plots and tared according to the number of \%,,:cd, In relation to the untreated plots. Curbit provided excellent cote;, '' i both annual grasses and broadlcal weeds. As noted in the table, control itn cantaloupes was 90% at 33 days after planting for all three Curbit rates tried. At 82 days, the 0.5- and 1.5-lb. rates provided 737o control and the 1.0-lb. rate gave 83% control. Control in watermelon plots was 63 and 73.o' respectively, for the 0.5- and 1.0-lb. Curbit rates and 88% for the 1.5-lb. rate, when rated at 33 days. The other materials generally gave poorer results, as shown by results in the table. Curbit offers seveial advantages over the other materials tested. It does not have the volatility problenis of Alarap, nor the need for niechanical ircorpora- tion and the residue problein of Prefar. Alanap must be watered in after applica- tion or the product evaporates. Prefar used alone niust be incoiporated into the soil, thus adding to the cost ot applica- tion. Another problem is that certain crops cannot be planted the next ciop year where Prefar has been used. Command gave good control of both annual grasses aid broadleat weeds. However, it caused phyrotoxicity to the melon plants, especially at the higher rates. This damage was characterized by an interveinal chlorosis, which did not kill but weakeiied the plants. In coitrast, Curbit caused ro obvious plant damage. Ratings of treatments in this test in- dicated that Curbit at rates of 1.0 and 1.5 lb. a.i. gave best weed control with no phytotoxicity problems. Norton is Protessor, Boyhan is Research Associate, anid Brown is Assistant Professor of Horticulturc, flollinigsworth is Superintendent of theN \o ih : atit I ituun r Suthtai It Alabana Agricultural Experiment Station T.P. MACK and R.H. SMITH CAREFUL MANAGEMENT METHODS NECESSARY TO CONTROL RESISTANT SOYBEAN LOOPERS THE SOYBEAN looper is the number one insect pest of soy- beans in Alabama. Its larvae reduce yield by eating leaves that are necessary to produce seed. Control of this pest is complicated by its habits. The soybean looper usually does not overwinter in Alabama, so adult moths must migrate into soybean fields during the growing season. Populations develop so rapidly (30-fold or greater increase in less than a week) that growers cannot use management practices that build up an abundance of natural enemies to provide control. The explosive population increase of the pest simply overwhelms the ability of natural enemies to provide adequate control. A further complication is resistance to insecticides that was reported in 1988 in Georgia, Louisiana, Mississippi, and Alabama. No currently registered insecticide consistently controls these resistant pests. However, use of Dipel? (a microbial control agent that causes a bacterial disease of loopers) in combina- tion with an insecticide showed promise in 1989 Alabama Agricultural Experi- ment Station research. Several registered and unregistered in- secticides were evaluated for soybean looper control during 1989 at Tallassee. Braxton soybeans that were planted July 1, using conventional tillage on 36-in. rows, were used for the comparisons. Plants had full-sized pods with small seeds in them when treated September 13. Treatments are listed in the table. Treatments were applied in water using a boom sprayer equipped with three nozzles per row operating at 40 p.s.i. and delivering 8 gal. per acre. In- sect populations were sampled from each plot 2 hours before treatment and at 2, 5, and 7 days after treatment. Most treatments reduced the number of soybean loopers 2 days after treat- ment, but Larvin? and Dimilin? were ineffective. The most effective treat- ments were Danitol? plus Orthene,? Danitol,? Kryocide,? Dipel plus Larvin, and Dipel plus Ambush? (rates listed in table). Populations of soybean looper in all plots appeared to decline greatly during the experiment, prob- ably because of pupation of larger individuals. Five treatments reduced the number of larvae at 5 days after application: Dipel, Kryocide, Danitol plus Orthene, Dipel plus Larvin, and Dipel plus Am- bush. None of the treatments gave results different from the untreated con- trol plots at 7 days after application. A large-plot demonstration trial in- cluded eight treatments of 5-acre field strips. The following treatments were applied September 12, using two nozzles per row operating at 40 p.s.i. and delivering 5 gal. per acre: Ambush + the synergist Ovasyn? (0.15 + 0.25 lb. active), Ambush + Dipel (0.15 lb. + 1.5 pt. formulated), Larvin (0.8 lb.), Lannate? + Dipel (0.45 lb. + 1.5 pt. formulated), Scout? + Dipel + the synergist PBO? (0.019 lb. + 1.5 pt. for- mulated + 1.0 lb.), and Orthene + Dibrom? + Dimilin (0.75 lb. + 1.0 lb. + 8.0 oz.). The Ambush + Ovasyn treatment resulted in 26 larvae per 3 row-ft. 3 days after treatment, the same as the un- treated plot. This compared with 8 larvae per 3 row-ft. for the Orthene + Dibrom + Dimilin treatment and 2 or fewer for the other treatments. Rapid control of the soybean looper may or may not be important, depend- ing on the age of the larvae when they are sprayed. About half of all leaf con- sumption by this insect occurs in the last 2 days before the larvae pupate, so it is important to prevent larvae from reaching this age. If most loopers in a field are 3/4 in. or larger, then most damage may have already occurred and a spray may not be profitable. Combinations of an insecticide such as Ambush, Lannate, or Larvin with Dipel were quite effective in both the small-plot and large-plot tests. Dipel ap- pears to be an excellent additive for im- proving control of looper larvae. The addition of a synergist such as Ovasyn or PBO did not improve control. The choice of an insecticide is especially critical because insecticidal resistance will likely continue. Use of an ineffective insecticide could make matters worse, by killing beneficial in- sects and accelerating development of insecticidal resistance. Mack is Associate Professor and Smith is Pro- fessor of Entomology. Alabama Agricultural Experiment Station M.L. ESLICK and G.R. McDANIEL SPERMATOZOA COUNTs AFFECT BROILER/BREEDER EGG FERTILITY AND HATCHABILITY B y ADAPTING a system of feeding male and female broilers separately, poultry- men have increased hatcha- bility by 5-6%ro. Despite this improve- meit, 15%o of all eggs set do not hatch. Recent Alabama Agricultural Experi- ment Station research indicates this is due to reduced spermatozoa counts, which cause decreased fertility, total hatch, and tertile hatch and increased total mortality and early embryonic mortality. As broiler breeders age, fertility declines due to both the decline in sperm cell output by the male and the decline in the ability of the female to retain viable sperm cells. With this decline in fert ilit',, embryonic mortality increases. However, the cause and effect relation- ship in the decline in fertility and in- crease in embryonic mortality has alavs been confounded with other variables, such as age, health, and phssiological state of breeders. Iherefore, a study was conducted to determine it levels of fertility are related to embryonic mortality. Ninety-six broiler breeder hens, 33 weeks of age and of approximately equal weight and production rate (71 0 /o), were assigned to wire cages at the den- sity of one hen per cage. A 16% protein, 2,867 kcal/kg breeder diet was hand- fed, and water was available at all times. Hens in the four treatments were ar- tificially inseminated (Al) with 25, 50, 100, and 200 million spermatozoa cells Ii a ixii t \iaii m n Variable I otal hahi I rrile haich.. Total miortality tark dead Mid dead .iic dcad Iatrls dead I5-21 dajs. 25 50 10 I'ct Pc. Pc 46 60 6 16 So 5 72 84 81 27 15 1 27 12 8. 0.2 2.1 4. 2 1.3 A 1-7 das; mid dead = 8-14 das; *4 - . t every 5 days throughout a 4-week period. Semen was collected and pooled immediately prior to Al and spermato- zoa numbers per Al were measured. Eggs were collected once daily and set twice weekly. On the 1 Ith day of incuba- tion the eggs were candled and the clears were removed and broken out to de- termine true fertility and embryonic mortality. At the end of 21 days of in- cubation, eggs that failed to hatch were examined to determine whe- iikH ]IN ther the failure to hatch was due to infertility or mortality. naiozoa cells Spermatozoa count had a 0 200 significant effeet on fertility t P and total hatch. Hens in- 87 6 79 setinated with the lowest 6 90 concentration had the lowest 6 96 fertility. A point of sper- 8 15 natozoa saturation was y 5 reached between 100 and 200 lait dead million, after which fertility decreased. And, of all eggs collected, the lowest spermatozoa counts resulted in the lowest hatch. At sper- matozoa counts of 100 million and 200 million, this comparison also reached a point of saturation at which the incre- ment of increase in total hatch decreas- ed to insignificance. Early embryonic death was affected by varying spermatozoa counts, with lower spermatozoa concentrations resulting in higher early embryonic death. Of the fertile eggs hatched, those with the lowest spermatozoa counts had the lowest hatch of fertility. Spermatozoa counts had a significant linear response to the total mortality of embryos. Total mortality included early, mid, and late embryonic death. As in- dicated by data in the table, lower spermatozoa concentrations resulted in higher total mortality. 1 slick is forner (raduate Student and Mcc. )aniel is Proessor of Poulrs Science. Alabuina Agricultural Experiment Station D.I. BRANSBY and J.T. EASON SHEEP OUTPERFORM CATTLE ON RYEGRASS PASTURE WEANED lambs were more productive than steers when grazing annual rye- grass pastures in spring. This was shown in an 84-day grazing ex- periment at the Sand Mountain Substa- tion near Crossville. The objective of this Alabama Agricultural Experiment Station test was to determine relative productivity and stocking equivalents of lambs and steers under grazing conditions. Marshall ryegrass was seeded at 25 lb. of seed per acre into a prepared seedbed in fall 1988. Potassium and phosphorus were corrected according to soil test. Nitrogen was applied as ammonium nitrate at a rate of 24 lb. per acre at planting and 64 lb. per acre in spring. Forage was allowed to accumulate un- til the beginning of April 1989 when grazing commenced. Pastures were grazed at stocking rates of 15, 20, 25, and 30 lambs per acre and 1.5, 2.0, 2.5, and 3.0 steers per acre. Lambs averag- ed 41 lb. at the start of the experiment and steers averaged 501 lb. Pastures were continuously grazed. Animals were weighed every 28 days and pasture height was measured periodically. Average daily gain (ADG) is usually higher at low stocking rates than at high stocking rates. However, in this study ADG showed little change with in- creased stocking rates, see table. This was probably due to substantial amounts of forage that were allowed to accumulate prior to commencement of grazing. Since the quantity of forage con- sumed by ruminants is related to their weight, meaningful comparison of ADG for lambs and steers requires an ap- propriate adjustment. At the beginning of this experiment, steers were 12.2 times heavier than lambs. Steer- equivalent ADG for lambs can therefore be calculated by multiplying mean lamb ADG (0.24 lb.) by 12.2. The value obtained (2.93 lb.) is 540% higher than the mean ADG for steers (1.90 lb.). This n~;:~F I'~e::?ii~i4~ P P I~ ~-%?~~ ~n. Ins: I. >1 *-& ,. J ,Mf higher efficiency of lambs is related partly to their ability to select higher quality leaves and reject stems because of their small mouths and nibbling- grazing action, compared to steers that depend more on tongue action to gather forage into their mouths. Gain per acre generally increased with stocking rate. Highest gain per acre for the 84-day grazing period was obtained at the highest stocking rate for both lambs and steers, see table. At these stocking rates, lambs provided 49% higher gain per acre than steers, sug- gesting that they are more efficient at harvesting both a high quantity and quality of forage when grazing. The greater efficiency of lambs in maximiz- ing the quantity of forage consumed may be related to less contamination of forage by excreta, and a tendency for sheep not to reject forage located near excreta as much as cattle do. Pasture height was generally greater and decreased more as stocking rate in- creased for steers as compared to lambs. However, at the highest stocking rates (30 lambs per acre and 3 steers per acre), pasture height was similar. This suggests that approximately 10 lambs can be car- ried in the place of one steer at this high level of pasture utilization. Results from the first year of this ex- periment showed that lambs were more productive than steers on both a per animal weight basis and a per acre basis. However, the grazing behavior of sheep and cattle tends to be complementary rather than competitive. Bransby is Associate Professor of Agronomy and Soils; Eason is Superintendent of the Sand \1nountain Sui b h tation. GAIN oF LAMBS AND STIERS GRAZNII RI iRAnSS Performance measure Lambs Steers Per acre stocking rate, no................ 15 20 25 30 1.5 2.0 2.5 3.0 Average daily gain, lb................... 0.24 0.24 0.21 0.27 1.84 1.81 2.15 1.81 Gain per acre, lb. .................... 302 403 441 680 232 304 451 456 Pasture height, in ...................... 7.6 5.7 5.8 6.2 1(0.3 8.2 7.3 6.04 Alabama Agricultural Experiment Station fst C ONIIERCIALI broiler feed is commonly pelleted, a process which can reduce wastage, sometimes increase nutrient availability, and usually improves live performance. Maintaining pellet in- tegrity is essential to realizing most of these benefits. Many feed additives are available to improve pellet stability, each varying in price, composition, and effectiveness. Lignosulfonates, by-products of the paper industry, have been widely used for over 25 years as binder agents. These compounds are available in sodium, calcium, or ammonium salt forms and contain a high percentage of various wood sugars and hemicelluloses. Previous studies done elsewhere have generally shown improvements in live performance from the use of ligno- sulfonates. However, measurements to corroborate effectiveness as a binder, possible alterations to nutrient content, and effects on carcass quality were absent. An experiment to address these inadequacies was conducted at the Alabama Agricultural Experiment Station using calciumlignosulfonate (CaLS) as a binder in feed for broilers. Broilers used for the study were reared with the sexes separated and mixed, then slaughtered at ages which would equalize their aver:c !i c cightl (males at 41 days, LivE PEIOKR, females at 47 days, IN s and combined sexes at 44 days). Feed- Treatment ing was performed Market during the summer Sex age, ( when the advantage of intact pellets and Males 41 the reduced "work" Females 47 Mixed 44 of eating would be Binding agents help maintain high pellet uality of poultry feed but may not improve usually remains in the body cavity after evisceration. Females have more fat remaining than males and the propor- tion increases with age. In all cases, fat increased when CaLS had been used. Enhanced pellet quality has been credited with improved live performance because birds do not have to work as hard or spend as much time at the feed trough to get sufficient amounts of feed. This energy savings can be used for growth. In this study, the increased productive energy arising from pellet stability led to additional body fat and adversely affected feed conversion. Feed protein must be sufficient if meat rather than fat is to be synthesized. Unfortunately, the finisher was balanced for a moderate environment, and the high temperatures led to protein being low relative to energy. This poor balance is believed to have been further exaggerated by improved pellet quality. Pellet stability is ordinarily valuable to broiler performance, particularly in hot weather. CaLS substantially im- proves pellet quality; however, this advantage did not result in better performance, most probably because feed protein had not been adjusted to effectively use the associated in- crease in productive energy. greatest. All feeds were based on corn, soybean meal, corn gluten meal, and poultry fat, which are ingredients of high nutritional value but often lead to poor pellet integrity. A crumbed starting feed was offered to all birds during the first 3 weeks. The subsequent finisher feed was pelleted, half with 25 lb. CaLS per ton added and half without the CaLS. Feed was sampled from the pen trough so that all aspects of handling would be encountered. Adding CaLS to the feed led to a 55% improvement of intact pellets, but did not alter nutrient content based on analyses. Amino acids associated with the crude protein (20.6%0) were similar with and without CaLS. Live performance of broilers receiving the CaLS-treated finishing feed did not parallel previous reports. Final body weights were not meaningfully improved by the better pellets for any of the birds in the test, see table. Furthermore, an increase in feed conversion occurred, which was also contrary to expectation. Yields of the carcass after processing and chilling were typically greater with females than with males, largely be- cause of their age difference at slaughter. The presence of CaLS and improved pellet quality with the final feed had no elffeet. \hdominal Ifat \NL ANII AfIxyrm I'r F I 1kO1 III Cll. nEl CARCAS oI- BROil. GIEN GA lHING )TI FomI 3 WiI .KS UN III MARI-I NIG WI 1H 5511 WIIH111 I Final weight Feed/lb. gain Carcass yield Abdominal fat Control +CaLS Control +-CaS Control +CaLS Control +CaLS Lb. Lb. Lb. 4.15 4.15 1.75 4.21 4.22 1.94 4.19 4.24 1.85 Lh. Pet. Pct. Pet. Pet. Acar and Revington 1.80 64.5 63.9 2.45 2.60 Ire Graduate Research 1.98 66.9 66.8 3.19 3.40 \sistants and Moran 1.86 65.1 65.6 2.54 2.57 ind Bilgili are Professors of Poultry Science. Alabama Agricultural Experiment Station J.E. DUNKELBERGER, G.W. GRIFFIN, R.S. MYRICK, and G.J. LYLES VANDALISM AND PROPERTY CRIME PLAGUE URBAN AND RURAL RESIDENTS.......... REPORTED crime is again in- creasing in the United States after a brief period of decline. Some 93 million homes, or one-fourth of all U.S. households, were touched by crime in 1988. Information published by the Alabama Criminal Justice Information Center revealed that this turn-around in crime rates occurred at mid-decade for Alabama. By 1986, all property crimes, including robbery, burglary, larceny, and motor vehicle theft, reflected higher rates than in the early 1980's. In 1984 and 1985, the Alabama Agricultural Experiment Station and Tuskegee University jointly conducted a statewide survey of Alabama households to determine their ex- periences with crime. Approximately 1,600 households provided information. For this report, each of Alabama's 67 counties was classified either rural (48 counties with populations less than the State average of 76.7 people per sq. mi. and containing no cities larger than 50,000), urban (14 counties with pop- ulations above the State average but containing no cities larger than 50,000), or metropolitan (5 counties in the State containing cities with populations larger than 50,000). Five distinct kinds of nonviolent household victimization were reported by respondents: (1) vandalism around the home, (2) theft of property from around but not in the home, (3) burglary or attempted burglary of home or building, (4) theft of property from household members while they were away from home, and (5) theft of a motor vehicle. As noted in the table, almost 30%7 of the sample Alabama households experienced some form of victimization at the hands of nonhousehold members in 1985. Vandalism of the house itself or any property around the house includes all attempted incidents whether or not property was actually damaged or destroyed. Acts of vandalism are more common in metropoli- Te of tan counties than in rural counties, even though more police Theft from arou and more frequent u home police patrolling exist in cities. Rural resi- dents are more likely ebers whi to experience vandal- from home ism the closer they live of a to a city. or committed Households that re- h :d .mbers ported incidents of vandalism rarely had their property losses Violent crime covered by insurance. Much of the cost asso- ciated with vandalism is in the frustration and psychological trauma experienced by individuals who have had their household property damaged or destroyed. About 12% of the sample households were victims of theft from around the home. Households in rural counties were less likely to experience such a crime than residents of either urban or metropolitan counties. Stolen items were not covered by insurance in 75% of the victimized households. Burglary or attempted burglary of the home or other buildings on the im- mediate property site was experienced by about 6% of the sample households. Rural residents were more likely to be burglarized than metropolitan residents, while urban households were most likely to be victimized. The most common theft experience involved the theft of personal property while a household member was travel- ing or living away from home. Metropolitan county residents were most likely to be victims of such crimes, but rural county residents also ex- perienced a high incidence. Property stolen from a person while away from home was covered by insurance only 10% of the time. Theft involving a motor vehicle or self-propelled farm machine receives much media attention but is a low- incident type of crime. The property in- volved in the majority of such incidents was either a car or truck. Urban residents were less likely to be victimized than either rural or metropolitan residents. In half of the incidents, the vehicle was covered by insurance. Data from the survey suggest that many crimes, especially those involv- ing the theft or damage of personal property, are not reported to law en- forcement agencies that collect and report official crime statistics. On the other hand, much of this unreported crime involves relatively minor incidents and modest dollar losses. Nevertheless, frustration and trauma may be serious. In any one year, a sizeable proportion of Alabama households feel the "sting" of vandalism and crime; 17% of Alabama households experienced an at- tempted theft of some type in 1988. Although there is less likelihood of be- ing victimized in rural areas, crime does exist and is increasing in the countryside. Dunkelberger is Professor and Griffin and Myrick are Graduate Research Assistants of Agricultural Economics and Rural Sociology; Lyles is Professor of Sociology, Tuskegee University. Alabama Agricultural Experiment Station 10 K.M. TILT, R.L. SHUMACK, and W.J. FOSTER SAVING NURSERY IRRIGATION RUNOFF OFFERS CONSERVATION AND PRODUCTION ADVANTAGES O VERHEAD IRRIGATION of a container nursery re- qurres about 20,000 to 50,000 gal. of water per acre detriment; per das ot irrigation. Unfortunately, sirulates 7)-8001'1 ot this water falls between or tainers are passes through the containers and can- area ot a not be used by the plants. Further, this ofte occe lost sater moves into underground nurseries. reserves or rurs oft. Not only is that sith live ssater lost, bt it prosides a vehicle for ing in cot carryirng chemical pollutants into surtace level in sat or ground sater. Thus, there are tswo out besseec compelling reasons to look for better ir- drip irrig, rigation riethods. needed, fir Some nurseries hase already im- Jaruary plemeruted consersatior measures to measuren address the concerns about overhead 1989. irrigation. One retiod adopted has L ive oa been to channel runoff into ioldine to catchi ponds where the sater is filtered and saucers os recy cled. Another technique is to use run-off, f drip irrigation sshere each container has averaged its osn water source. Drip irrigation was tairt, pr es cuts swatei loss betseet pots, but saucer. I excess ssater still passes through the con- 1.9 in. svhr tairers into the ground sater systen to ard 5.1 ft proside tle potential for pollution. drip irriga Research ot the A\labara Agricultural increase f Eiperimienr t Satron is esaluating an additional conservation measure for use Height, fi swith drip irrigation. In this project at the 10 Ornamertal H orticultore Substation, v Miobile, water roi drip irrigation pass- 8 r v lug thr ougi inairers is being caught in saucers rather than being allosw ed to 6 run off. I-he potential benefits of catching the 4 leachate or swater passing through the container are being esaluated in the 2 protect, along swith some ot her aspects of such a s5stem. 0 One goal was to deteriine shether a S constant swater lesel maintained in a FIG. 2. EffE saucer beneath a container sould be growth of I Alubunin Agricultural L sperilment Station II to grosth. This treatment the situation in which con- in a water-filled depression nursery. IThis type situation irs wit h detrimental effects in Three treatments were used )ak and Shumard oak grow- ntainers: (1) constant w5ater icer, (2) saucer allowed to dry n irrigations, and (3) standard ation in each container as gure 1. The trees were planted 18, 1988, and grosth ents were made October 10, k showed growth adsantages tg and holding water in the er standard drip irrigation and igure 2. Height and caliper 7.2 ft. arid 1.9 in. wshen wsater aired at a constant lesel in the its corpares with 6.1 ft. and en saucers wserc allossed to drv . and 1.4 in. Irorm standaird tion and run-off. The growth 0111 use of saucers to catch Wet = maintain constant water level in saucer Dry = allow saucer to dry berween irrigations Control= water as needed through drip Ingatl - U:i. humard oak Live oak ects of irrigation treatments on height ive oak and Shumard oak. water would boost wholesale salue about $8 per tree. Another phase of the study compared 15-gal. and 30-gal. containers for grow ing the trees. Ten trees in each size con- tainer were produced under each of the three irrigation treatnents already described. There was a clear advantage to grow- ing the trees in 30-gal. containers conpared to 15-gal. containers. Height growth differences favored the larger container by 0.8 ft. for live oak and 1.6 ft. for Shumard oak, figure 3. Caliper size of live oak increased from 1.5 in. in the 15-gal. container to 1.9 in. it the 30-gal. container. Shumard oak show- ed no difference in caliper between the two container sizes. The size dif'erences resulting from the larger containers translate into $5 to $10 per plant value differences. Iill \ Iloia t'ric',oor and 5Strimiwkk is tteard ofthe Ontmeta Horl~~i r tiiiicutr Suibsitaiion. Height, ft. 8 ? 30-gal container S15-gal container 41 2I Shumard oak Live oak FIG. 3. Effects of container size on growth of live oak and Shumard Oak. ) 5... .7 A.~~T FIG. 1. Drip irrigation treatments: (1) irrigated as deemed necessary, no saucer; (2) saucer allowed to dry out between ir- rigations; (3) saucer maintained at constant water level. ab~F ~i~ ZI_ -- .I*"1, iai J.T. FOLEY and G.J. KEEVER BRANCHING OF LESSER PERIWINKLE INCREASED BY GROWTH REGULATORS L ESSER periwinkle is one of the most common vining ground- covers in this region because it adapts well to many different settings, including shaded areas, and is fast-growing. Its growth is characterized by long runners and little lateral shoot development. Lesser periwinkle plants are typically sold by the number of runners on the plant. Many such crops are potted several plants per pot or pruned repeatedly during production to produce well-branched, high quality plants. The first approach requires more plant material than using one plant per pot, while the latter is labor intensive. An Alabama Agricultural Experiment Station study was instituted to determine the effectiveness of several plant growth regulators in inducing lateral budbreak and elongation of lesser periwinkle. Cuttings of lesser periwinkle were in- serted in 36-cell flats with a Pro-Mix BX ? medium and placed in a double- layer polyethylene greenhouse under intermittent mist for 11 weeks. Rooted cuttings were pruned to three nodes each; all side and basal shoots were removed. The following foliar Comparison of branching of untreated (left) and Promalin- treated periwinkle. spray treatments were then applied to the plants: Promalin? [a mixture of the synthetic cytokinin BA (6-benzyladenine) and gibberellins A,A,] at 125, 250, and 500 parts per million (p.p.m.). Atrinal? (dikegulac so- dium) at 1,000, 2,000, and 3,000 p.p.m. BA at 62.5, 125, and 250 p.p.m. Accel? (another syn- thetic cytokinin) at 62.5, 125, and 250 p.p.m. Sprays were applied just prior to runoff in a volume of about 2.0 ml (milliliters) per plant. Buffer X? was added as a surfac- tant at 0.2%o to BA, Promalin, and Accel. Plants were fertilized weekly with 200 p.p.m. nitrogen from 20-10-20. A control group of plants was treated iden- tically to these except no growth regulators were applied. Promalin treatments increased runner number and increased runner length up to 250 p.p.m. Atrinal delayed runner production through week 4, but numbers increased thereafter and run- ner length decreased as Atrinal rate increased. BA did not increase runner number and increased rates of BA tended to increase runner length. Accel did not increase runner number, and increased rates of Accel decreased run- ner length. Based on results of the first experi- ment, a second test was initiated to determine appropriate rates of the most effective branching compounds from the initial test. Since Atrinal and Pro- malin both increased runner number, they were selected for comparison in the second test. Foliar spray treatments consisted of Promalin and Atrinal at 250, 500, and 1,000 p.p.m. PRIMARY RuINNER NUMBER AN) LENGTHS OF LSSER P RIWINKL inal Final Treatment runners runner length No. In. Promalin 250 p.p.m............. 4.2 14 500 p.p.m.......... .... 5.1 12 1,000 p.p.m........... 7.1 12 Atrinal 250 p.p.m............. 3.3 14 500 p.p.m............. 2.3 14 1,000 o.p.m ........... 3.0 14 U ntreated ............... 2.5 15 'Runners produced from initial 3-node cutting. Treatments, excluding pruning, were applied a second time without pruning 6 weeks after the initial application. Data were taken at 2-week intervals and included primary runner number and length, secondary runner number (from primary runners), and basal runner number (from the growth medium). Final data also consisted of a node count of primary runners as well as a measure of the three longest secondary runners. The results of each chemical treat- ment in the second study showed that Promalin increased primary runner number from the three-node cutting, increased secondary and total runner number, and decreased primary runner length while secondary runner length increased as rate of Promalin increased. Atrinal did not promote runner produc- tion or runner elongation, as shown in the table. Treatment with Promalin resulted in light to severe chlorosis, especially at higher rates. Plants eventually developed normal foliar color and the majority of secondary runners survived. These results indicate that Promalin at 500 to 1,000 p.p.m., using two applications about 6 weeks apart, is effective in inducing lateral branching of runners of lesser periwinkle. Foley is Graduate Student and Keever is Associate Professor of Horticulture. Alabama Agricultural Experiment Station J.W. ODOM BORON FERTILIZER APPLICATIONS MAY NOT BE NECESSARY FOR IRRIGATED CORN C OASTAL Plain soils of Alabama are low in extrac- table boron and it is often assumed that irrigated corn needs boron applications for maximum yield. To evaluate this assumption, an Alabama Agricultural Experiment Sta- tion field test was conducted on a Goldsboro soil at the E.V. Smith Research Center near Shorter. Three boron treatments (0, 2, and 4 lb. boron per acre) were applied as four split applications each year to avoid leaching the boron out of the corn's rooting zone. Nitrogen and sulfur fer- tilizers were applied at the same times as the boron fertilizer. All phosphorus, potassium, and zinc fertilizers and lime were incorporated before the test was planted each year. The treatments included: no irrigation (rainfall only); drip irrigation with control tensiometers at a depth of 6 in. (topsoil or AP horizon); and drip irrigation with control ten- siometers at a depth of 13 in. (sub- soil or BA horizon), as shown in the figure. The least water was ap- plied during 1985 when the amount and distribution of rain- fall during the growing season were both favorable. Because irri- gation water was applied under the crop canopy, the actual amounts of water required were low. There was little difference in corn yield between irrigation con- trolled by 6-in. and 13-in. place- ment of tensiometers. However, drip irrigation increased shelled corn yield in all years, see table. Shelled corn yields from the ir- rigated plots decreased the second and third years of the test. These decreases with time may be due to a build-up of disease organisms and parasites in the test area. Crop yields are often increased by crop rotation in Alabama. Boron application did not in- irri crease shelled corn yield either with tior or without drip irrigation. E-L(isc However, boron fertilizer did increase the ear-leaf boron concentration at silking from 14 to 26 p.p.m. This increas- ed boron concentration in No irriga 0 ..... the plants shows that the 2....... boron fertilizer was available 4. .... . to the corn. The extractable Drip irri boron in the topsoil of the 2....... Goldsboro soil (0.22 p.p.m.) 4...... . Drip irril is in the range where some 0....... soil testing laboratories 2....... recommend boron appli- 4....... cation. Since excessive amounts of boron can be toxic to crops and since boron ac- cumulates in this soil, large annual ap- plications of boron could be a problem. The grain yield of corn growing on this Goldsboro soil in central Alabama was increased by drip irrigation, but boron application did not increase yield. Total, in. 70 60 Rainfall 50 _. Irrigation 40 30 20 )F IRRIGATION AND BORON FERTILIZER ON SHELLED CORN YII D IN CENIRAL ALABAMA Yield per acre Boron per acre, lb. 1985 1986 1987 Av. 1985 1986 1987 Av. Bt BH Bi Bu_ ition .............. 1. 65 20 114 100 1.80 16 125 107 .. 184 26 107 106 gation, 6 in. tensiometers ......................... 156 229 156 214 261 202 158 207 . 245 223 168 212 gation, 13 in. tensiometers .............. . 227 231 157 205 ............ .. ...... 236 210 173 206 .. ... ... ........ 170 212 179 187 Application of excessive amounts of fer- tilizers such as boron can be avoided to both reduce cost of crop production and avoid possible contamination of human and livestock drinking water. Odom is Associate Professor of Agronomy and Soils. 198s 1986 181/ 1985 1986 1987 1985 1986 1987 No irrigation Drip irrigation Drip irrigation 13 in. tensiometers 6 in. tensiometers gation treatments during three consecutive crop years varied depending on amount and distribu- of rainfall received during those years. Alabama Agricultural Experiment Station D.A. COLEMAN, F.F. BARTOL, J.G. FLOYD, R.W. PEACOCK, and J.B. MAYFIELD ESTROUS SYNCHRONIZATION AND AI OFFER EFFECTIVE BREEDING PROGRAM C OMBINING estrous syn- chronization with artificial insemination (AI) offers a progressive breeding program that is suited to both full-time and part- time beef producers. AI provides producers easy and affordable access to the best bulls in the country, while estrous synchronization increases effi- ciency of heat detection (with no reduction in first-service conception rate) and allows cows to conceive earlier in the breeding season. Some producers need to concentrate labor associated with estrous syn- chronization and heat detection from Monday through Friday when hired help is available. Other producers who work off-farm during the week might wish to confine most of these activities to the weekend when they are at home. Beef producers often fail to utilize estrous synchronization with AI because it can be awkward to fit into their work schedules; however, several "package" estrous synchronization and AI breeding programs have been developed that allow maximum pregnancy rates from a minimum input of time and labor. An Alabama Agricultural Experiment Station study tested one of these estrous synchronization-AI programs using 76 crossbred beef cattle (Angus-Hereford 1 , .1 ,,- , t . 1 .. I- Hill. This brood cow herd had been utilized previously in a controlled breeding program and, as a result, the herd had a well defined 45- to 60-day October-November fall calving season in 1988. All cows were therefore at least 45 days postpartum by the beginning of the January 1989 breeding season. As part of a routine herd health program, cows had been examined by a veterinarian and were reported to be reproductively normal and cyclic. Half of the herd was assigned to an estrous synchronization program and the other half to a control treatment. The synchronization program was called a "Monday morning" program because it permitted treatments and observations to take place during the normal Monday-Friday work week. This breeding program included three steps: 1. Each cow in the treatment group was injected with a standard dose of 25 mg (milligrams) Lutalyse? on Monday, January 9, and observed for heat 30 to 45 minutes twice daily (dawn and dusk) Tuesday morning through Friday even- ing (8 heat checks over 4 days). 2. The following Monday morning (January 16) any cow in the treated group that had not been observed in heat and bred the previous week re- ceived a second injection of Lutalyse and cows were checked for heat Wednesday morning through Friday evening (6 heat checks over 3 days). chronized 3. Unbred cows received a 38 third injection on Monday (January 23) and any cow in 8 the treatment group that had not been observed in heat and bred between Wednesday 21 morning and Thursday even- ing was bred at an 80-hour livided b timed breeding Thursday palpation evening (January 26; 4 heat X checks over 2 days). Cattle in the synchronized group were observed for estrus a total of 18 times over 9 days and had three opportunities to be inseminated during the 15-day treatment period from January 9 through 26. Control cows were observed for heat twice daily (dawn and dusk) for 3 weeks from the evening of January 9 through January 30 (43 observation periods over 22 days). All cows in the study were ar- tificially inseminated once by one of two AI technicians 12 hours after onset of estrus. Cows in both groups were later turned in with a bull. Cows were palpated for pregnancy 35-60 days after their single AI service. Analysis of these results showed that the synchronized cows required fewer than half the number of days and obser- vation periods required for detecting estrus in the control group, see table. No heat detection or breeding was required during weekends for the synchronized group. Although a slightly greater percentage of control cows was observed in heat during the trial period compared to synchronized cows, cows in both treatment groups had efficient first- service conception rates of approxi- mately 78%. This compares to an estimated average of only 60 to 70% conception rate to a single natural service by a bull. Control cows were detected in estrus throughout the 22-day trial period. By comparison, the majority of synchro- nized cows were observed in estrus, bred, and conceived after the first syn- chronization treatment. On the average, synchronized cows conceived nearly 1 week earlier than control cows. This means synchronized cows will calve a week earlier than control cows and, ultimately, their calves might be ex- pected to weigh more at a set marketing date. Synchronized cows also will be further along in their postpartum period and should be in better condition to con- ceive during the next established breeding period. This could also result in a tighter calving schedule since these cows would be ready to breed earlier during the next year's breeding season. Coleman is Assistant Professor, Bartol is Associate Professor, and Floyd is Associate Pro- fessor of Animal and Dairy Sciences; Peacock is Herd Supervisor and Mayfield is Research Techni- cian of the Piedmont Substation. Alabama Agricultural Experiment Station14 M.G. PATTERSON, W.B. WEBSTER, D.P. MOORE, and L.W. WELLS PREPLANT 2,4-D CONTROLS HORSEWEED IN MINIMUM TILLAGE ORSEWEED (mares tail) is a common annual weed in fields that have not been till- ed and causes problems when these fields are planted to cotton using minimum tillage. Seed are widely distributed by the wind from late fall to early spring. After germination, they form a small rosette of basal leaves at the soil surface, then a leafy stem up to 6 ft. tall grows from the rosette and ter- minates in a flowering panicle. Currently used preplant foliar-applied herbicides can be effective, but the cost is relatively high, and these materials must be applied before weeds reach 12 in. tall to achieve maximum efficacy. Therefore, research was initiated by the Alabama Agricultural Experiment Sta- tion in 1987 to evaluate preplant ap- plications of 2,4-D amine as an alter- native, reduced-cost herbicide in minimum tillage cotton. Four field tests were conducted to evaluate 2,4-D preplant at 0.5 and 1.0 lb. active ingredient per acre, applied February 1, March 1, or April 1, prior to planting cotton during the last 2 w eeks of April. One trial was conducted at the Tennessee Valley Substation, Belle Mina, in 1987, and one trial each was conducted in 1988 at the Tennessee Valley Substation; Prattville Experiment Field, Prattville; and Wiregrass Sub- station, Headland. After minimum tillage cotton planting, each trial was sprayed with a mixture of Cotoran,? Prowl," and Gramoxone? for general weed control. Plots were not cultivated. Horseweed control and potential crop injury caused by 2,4-D carryover were cvaluated at Belle Mina in both years. Crop inj ury was evaluated only at Pratt- ville and Headland since no horseweed was present in these tests. Application of either 0.5 or 1.0 lb. per acre 2,4-D at Belle Mina provided ex- cellent horseweed control and no visible crop injury at the February 1 or March I dates in 1987, table 1. Delaying 2,4-D applications until April 1 in 1987 resulted in phytotoxicity to the cotton crop and reduced horseweed control. Applica- tion of 1.0 lb. per acre in 1988 provided excellent con- trol of horseweed at all dates and no crop injury was observed. April applications at the low rate resulted in less control, primarily because weeds were too large at this date. Crop injury at Prattville and Headland was not observed, regardless of rate or time of application. Seed cotton yields from 2,4-D-treated plots were equal to the control plots at both Prattville and Head- land in 1988, table 2. Seed cotton yields at Belle Mina were higher from 2,4-D- treated plots than the control in 1987 and 1988. This was probably due to the horseweed competition from uncontrolled plants infesting the control plots. These results indicate that horseweed can be controlled effectively without injuring cotti 1.0 lb. per acre 2,4-D amine either February 1 or March 1, w ton is planted in late April. ii', Pion iia.i 2,4-D Vi Hi i H Mitt Crop injury, by Weed contro. b Rate acre application date application date I h Mir Anr [erh \lnr .Xr, 0.5 lb. 0 lb... Control . 0.5 lb. . 1.0 lb. Control P. Pct. Pc. Pc. P. Pc 1987 0 0 5 99 99 84 0 0 23 99 99 95 0) 65 1988 0 0 0 7 83 6 0 0 0 99 98 98 0 -) I \ii 2. Li FL( i ri 2,4-D Piiiii Hi Aridi i, viiNN iN Siiii Cm 1UN Yi IU Seed cotton sield/acre, by Rate/acre application date Feb. Mar. Apr. A \ Lb. Lb. Lb. l.h. 1987 Belle Mina (15 lb. .......... .. 1,656 1,744 1,613 1,671 1.0 lb. ... .... 1.591 1.70 1,337 1.543 (ontrol .. . .... 1 1988 Belle Mina 0.5 Ih. 836 799 966 867 L1 lb. ..lb 611 1,090 1,177 959 Coittrol . .......... 414 Prattville SIh. .......... 2,398 1,976 2,057 2,144 1.0 lb. ........... 1,853 2.369 2,463 2,228 Control ... ......... - - 2,507 Headland 0.5 lb. . .......... 1,444 1,992 1,888 1,775 1.0 lb. . ........... 1,863 1,904 2,05 1,941 Control .......... . .. 1,742 Patterson is Assistant Professor of Agronomyx on using and Soils; Webster is Superintendent of the Ten- applied nessee Valley Substation; Moore is Superintedent of the Prattville Experiment Field; Wells is Associate Superintendent of the Wiregrass Substation. ~il. 1-: B. d Minimum tillage plantings of cotton may require special preplant treatments to control horseweed. AIahumu Agriculturul Experinent Station ~- " '~'" "~ J.L. DUSI ALABAMA BLACK BEARS LIVE IN HARMON WITH MAN T HE BLACK BEAR, the largest carnivore in the Eastern United States, is a notable part of Alabama's wildlife heritage. Bears were once common in the State, but as forests were cut and agriculture developed, bears became isolated in the swamps and forested river bottoms mainly along the Mobile, Tensaw, Tom- bigbee, and Alabama rivers in southwestern Alabama. Though the recent return of agricultural land to forests has enlargened the statewide range of black bears, most Alabamians will never see one, because these animals are so shy. Because of increased highway mor- tality of bears in the State and newspaper reports of sightings, the Alabama Agricultural Experiment Sta- tion, in cooperation with the Alabama Department of Natural Resources, con- ducted a black bear ecology study. Most of the field work was done in a 25-sq.- mi. area located northwest of Saraland in Mobile County. Five bears were trapped during the study and were sedated, measured, and fitted with radio collars, as shown in the photograph. A small tooth was ex- tracted from each to help determine the bears' ages. The radio-tagged bears were tracked and their winter and summer home ranges determined. The habitat where bears were most frequently found was analyzed and their food habits studied by examining their fecal pellets. The largest summer home range (8 sq. mi.) was that of a 2-year-old male. The summer range for four females varied from 0.9 to 7.2 sq. mi. Winter ranges for all bears varied from 0.1 to 0.4 sq. mi. The bears did not hibernate in winter, but stayed in the swamps, moving little, unless disturbed. They were not typical carnivores because they fed on acorns, "T'I -;' - Young black bear that was trapped, sedated, and fitted with a radio transmitter. berries, or fruit during most of the year, and ate many insects, especially beetles. The key to bear abundance in southwestern Alabama appears to be the extensive area of swamps, with thick shrubby undergrowth of "titi" that is impenetrable without using a machete. Titi makes a safe retreat for bears, but unfortunately, in Alabama, it occurs only in swampy areas in southwestern counties. Despite increasing numbers and distribution, black bears in Alabama are ALABAMA AGRICULTURAL EXPERIMENT STATION AUBURN UNIVERSITY AUBURN UNIVERSITY, ALABAMA 36849-5403 Lowell T. Frobish, Director POSTMASTER-Address Correction Requested still mainly confined to the southwestern counties. Bears statewide are protected by State game laws and violators have been prosecuted. Results of the AAES study indicate that human contact with bears is limited to cases in which bears seek food from open garbage, honey from bee hives, or stored corn. In such cases, electric fences have proven effec- tive in keeping bears out. Dusi is Professor of Zoology and Wildlife Science. NON-PROFIT ORG. POSTAGE & FEES PAID PERMIT NO. 9 AUBURN, ALA. M v4