1991 Regional Cotton Fusarium Wilt Report February 1992 Agronomy and Soils Departmental Series No. 159 Alabama Agricultural Experiment Station Lowell T. Frobish, Director Auburn University Auburn University, Alabama 1991 REGIONAL COTTON FUSARIUM WILT REPORT' W.C. JOHNSON' Cotton cultivars and elite breeding lines submitted by 21 cooperators were evaluated for fusarium wilt resistance under field conditions at the Plant Breeding Unit, Tallassee, Alabama. These entries were grown on an Independence loamy fine sand highly infested with both the fusarium wilt fungus (Fusarium oxysporum) Schlect. f. vasinfectum [Atk.] (Snyd. & Hans.) and root-knot nematodes (Meloidogyne spp.). Plots were 40-inch-wide bedded rows, 30 feet in length, separated by 6-foot alleys. Four replications of the test entries and checks, arranged in a block design, were evaluated. Both susceptible (Rowden) and resistant (Auburn 56) cultivars were included as checks. Auburn 56 was developed at Auburn and released in 1953. It was used as the resistant check in the Regional Fusarium Wilt Test for many years. However, it was replaced when it was no longer grown commercially. Auburn 56 is again being used as the resistant check because, although obsolete, it is the most consistently resistant cultivar available. Rowden was planted in row 5 and every tenth row thereafter (15, 25,...,225) and Auburn 56 in row 10 and every tenth row thereafter (20, 30,...,220) throughout the test. Plots were planted May 3. Initial plant counts were made on June 21. Wilted plants were also 'This report is a joint contribution between USDA-ARS, Crop Science Research Laboratory, Mississippi State, Mississippi, and the Alabama Agricultural Experiment Station, Auburn University, Alabama. 2 Professor of Agronomy and Soils counted and removed on that date. Later, wilted plants were counted and removed on July 8, July 19, August 6, and August 13. The remaining live plants were also counted and recorded on August 13. Percent wilted plants were then determined and mean wilting for a given entry calculated. Average wilting of the susceptible Rowden was 85, 83, 91, and 88 percent for the four replications (87 percent average). Corresponding wilt percentages for the resistant check, Auburn 56, were 8, 8, 30, and 39 (21 percent average). Critical evaluation of a given entry should be made relative to the checks closest to the entry within each replication. Evaluation of breeding process or evaluation of entries over years should be made only between the relative value of this entry and that of the closest susceptible check rows for each year. Entries submitted by W. C. Johnson are commonly grown cultivars or advanced commercial materials and are listed by name. Entries submitted by other cooperators are listed by their coded numbers. Additional information regarding the genetic background of a specific coded entry should be obtained from the named cooperator. ACKNOWLEDGMENT The author expresses appreciation to A.J. Kappelman, Jr., retired, for advice and technical assistance in conducting the test and preparing this report. Information contained herein is available to all persons without regard to race, color, sex, or national origin. -4- 1991 FUSARIUM WILT TEST PLANT BREEDING UNIT, TALLASSEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 1 Jim Mitchell, Stoneville Pedigreed Seed Co., Inc., Box 167, Stoneville, MS 38776 001 JFM 1............. 55 9 64 91 55 002 JFM 2.............. 57 12 97 85 63 003 JFK 3.............. 84 40 91 91 76 004 JFM 4.............. 23 12 96 33 41 005 ROWDENW............ 89 95 100 76 90 006 JFM 5.............. 11 3 71 50 34 007 JFM 6.............. 62 44 96 38 60 008 JFM 7................89 5 87 15 49 009 JFM 8.............. 23 38 43 77 45 010 AUBURN 56.......... 5 5 18 6 8 2 C. Wayne Smith, Dept. of Soil & Crop Sciences, Texas A & M Univ., College Station, TX 77843-2474 011 86T2-2............. 3 5 14 36 14 012 86T2-12............ 61 18 79 85 61 013 86H3-16............ 51 18 87 86 60 014 86G3-300.......... 11 10 77 64 40 015 ROWDEN ............. 86 84 91 100 90 016 86SD-46............ 99 100 100 99 100 017 86SD-30............ 35 57 76 78 62 018 86G3-1............. 22 14 87 80 51 019 90C-19............. 1 21 46 17 21 020 AUBURN 56.......... 10 13 7 33 16 3 Robert R. Bridge, Delta Branch Expt. Sta., Mississippi State Univ., P.O. Box 197, Stoneville, MS 38776 021 RRB 1.............. 4 34 13 34 21 022 RRB 2.............. 8 99 62 85 64 023 RRB 3.............. 5 25 62 88 45 024 RRB 4.............. 13 50 38 54 39 025 ROWDENW........... 91 77 94 99 90 026 RRB 5.............. 5 34 38 75 38 027 RRB 6............. 2 37 22 82 36 028 RRB 7.............. 1 5 9 86 25 029 RRB 8.............. 2 78 4 90 44 030 AUBURN 56.......... 2 27 8 76 28 -5- 1991 FUSARIUM WILT TEST PLANT BREEDING UNIT, TALLASSEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 4 Keith R. Jones, Delta & Pine Land Co., P.O. Box 157, Scott, MS 38772 031 DPL 1.............. 10 94 75 100 70 032 DPL 2.............. 12 62 11 100 46 033 DPL 3.............. 32 100 78 87 74 034 DPL 4.............. 2 38 55 98 48 035 ROWDEN............. 29 84 100 87 75 036 DPL 5.............. 0 12 45 100 39 037 DPL 6.............. 0 20 27 44 23 038 DPL 7.............. 13 100 68 85 66 039 DPL 8.............. 43 78 38 76 59 040 AUBURN 56.......... 7 17 8 49 20 5 Richard F. Holland, AgriPro, P.O. Box 427, Axtell, TX 76624 041 1.................. 80 81 37 95 73 042 2.................. 5 84 28 86 51 043 3.................. 16 52 7 98 43 044 4.................. 11 69 25 79 46 045 ROWDEN ............. 41 88 17 99 61 046 5................. 6 50 17 100 43 047 6.................. 18 23 11 100 38 048 7.................. 9 13 4 93 30 049 8.................. 12 37 17 100 42 050 AUBURN 56.......... 6 14 12 97 32 6 Richard Sheetz, Cargill Hybrid Seeds, Box 2, Aiken, TX 79221 051 1.................. 6 39 21 85 38 052 2.................. 8 23 55 92 44 053 3.................. 67 64 85 84 75 054 4.................. 13 12 65 94 46 055 ROWDEN............. 98 65 98 84 86 056 5.................. 31 18 75 89 53 057 6.................. 1 15 10 23 12 058 7......:.-........... 5 7 17 20 12 059 8.................. 3 2 29 23 14 060 AUBURN 56.......... 3 3 43 37 22 -6- 1991 FUSARIUM WILT TEST PLANT BREEDING UNIT, TALLASSEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 7 A. L. Germany, Stoneville Pedigreed Seed Co., Inc., Box 167, Stoneville, MS 38776 061 1.................. 41 21 83 85 58 062 2.................. 63 11 99 100 68 063 3.................. 42 25 95 100 66 064 4.................. 33 20 100 61 54 065 ROWDEN ............. 80 77 98 93 87 066 5.................. 16 20 44 59 35 067 6.................. 0 14 36 71 30 068 7.................. 7 31 50 15 26 069 8.................. 6 4 81 15 26 070 AUBURN 56.......... 1 2 39 23 16 8 John Green, Seed Source, Inc., 106 4th Street, Leland, MS 38756 071 SS1................ 0 13 20 2 9 072 SS2................ 16 11 54 1 20 073 SS3................ 8 17 88 7 30 074 SS4................ 57 59 67 14 49 075 ROWDEN............ 87 77 89 75 82 076 SS5................ 11 14 73 25 31 077 SS6 ................ 3 7 79 37 32 078 SS7................ 6 2 38 9 14 079 SS8................ 45 40 96 39 55 080 AUBURN 56.......... 13 5 27 10 14 9 Kamal M. E1-Zik, Dept. of Soil & Crop Sci., Texas A & M Univ., College Station, TX 77843-2474 081 KME-1.............. 15 5 49 37 26 082 KME-2............. 10 2 11 8 8 083 KME-33............. 97 57 98 92 86 084 KME-4 ............ 39 17 77 41 44 085 ROWDEN............ 88 62 97 99 86 086 KME-5.............. 17 11 28 19 19 087 KME-6.............. 34 18 84 62 50 088 KME-7.............. 43 4 55 41 36 089 KME-8.............. 32 17 96 99 61 090 AUBURN 56.......... 12 2 20 11 11 -7- 1991 FUSARIUM WILT TEST PLANT BREEDING UNIT, TALLASSEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 10 Peggy Thaxton, Dept. of Soil & Crop Sci., Texas A & M Univ., College Station, TX 77843-2474 091 PMT-1.............. 12 52 69 47 45 092 PMT-2.............. 16 12 62 88 44 093 PMT-3.............. 36 23 94 66 55 094 PMT-4.............. 23 34 91 69 54 095 ROWDEN............. 96 59 96 85 84 096 PMT-5.............. 16 9 100 72 49 097 PMT-6.............. 18 16 84 97 54 098 PMT-7.............. 31 27 75 93 56 099 PMT-8.............. 80 64 99 93 84 100 AUBURN 56.......... 2 8 25 62 24 11 Warner D. Fisher, Chembred, Inc., Rt. 3, Box 750, Maricopa, AZ 85239 101 9................. 67 97 67 78 77 102 10..0............. 92 34 79 100 76 103 11................. 56 89 88 76 77 104 12................. 96 57 18 75 62 105 ROWDEN............. 100 100 95 71 92 106 13................. 44 69 27 96 59 107 14................ 20 14 44 65 36 108 15............... 38 17 22 84 40 109 16........... ..... 037 45 51 95 57 110 AUBURN 566...... 3 14 31 94 36 12 Verlin Boeder, Chembred, Inc., Rt. 3, Box 750, Maricopa, AZ 85239 111 1.................. 12 31 4 85 33 112 2.................. 89 64 13 99 66 113 3.................. 96 39 28 99 66 114 4.................. 13 31 32 97 43 115 ROWDEN...............96 100 94 100 98, 116 5.................. 60 75 69 94 74 117 6.................. 51 72 50 100 68 118 7.................. 20 24 81 95 55 119 8................ 45 34 63 100 60 120 AUBURN 56.... 17 7 12 50 22 -8- 1991 FUSARIUM WILT TEST PLANT BREEDING UNIT, TALLASSEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 13 Shelby H. Baker, Univ. of Ga. Coastal Plain Station, Tifton, GA 31793 121 Ga-1............... 21 9 94 2 32 122 Ga-2............... 79 20 86 95 70 123 Ga-3............... 14 7 89 99 52 124 Ga-4............... 35 20 99 88 60 125 ROWDEN............. 96 93 100 99 97 126 Ga-5............... 24 12 88 90 54 127 Ga-6............... 13 10 85 23 33 128 Ga-77............. 11 46 55 11 31 129 Ga-8............... 19 13 30 84 36 130 AUBURN 56........... 5 5 15 36 15 14 Bobby Phipps, Agrigenetics Co., 13974 W. Van Buren, Goodyear, AZ 85338 131 Ex. 1...... ........ 3 8 3 14 7 132 Ex. 2.............. 2 1 16 12 8 133 Ex. 3.............. 15 7 58 7 22 134 Ex. 4.............. 18 11 31 73 33 135 ROWDENW........... 98 93 99 95 96 136 Ex. 5.............. 1 9 14 15 10 137 Ex. 6.............. 6 4 62 19 23 138 Ex. 7.............. 3 3 15 5 6 139 Ex. 8.............. 5 0 10 8 6 140 AUBURN 56.......... 0 12 36 6 14 15 Laval M. Verhalen, Dept. of Agronomy, Oklahoma State Univ., Stillwater, OK 74079-0507 141 OKLA-1............. 1 1 48 14 16 142 OKLA-2............. 6 6 13 8 8 143 OKLA-3............. 22 27 47 7 26 144 OKLA-4............. 17 49 48 13 32 145 ROWDEN............. 63 99 97 61 80 146 OKLA-5............. 4 18 2 0 6 147 OKLA-6............. 18 17 13 6 14 148 OKLA-7............. 5 6 14 1 6 149 OKLA-8............. 1 13 70 36 30 150 AUBURN 56.......... 6 14 18 6 11 -9- 1991 FUSARIUM WILT TEST PLANT BREEDING UNIT, TALLASSEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 16 D. Steven Calhoun, Dept. of Agronomy, Louisiana State Univ., Baton Rouge, LA 70803-2110 151 1.................. 6 5 43 4 14 152 2.................. 22 14 76 7 30 153 3.................. 4 6 46 2 14 154 4.................. 20 6 64 6 24 155 ROWDEN............. 96 90 99 70 89 17 Fred Bourland, Dept. of Agronomy, University of Arkansas, Fayetteville, AR 72701 156 FB-1............... 0 5 100 16 30 157 FB-2............... 9 0 100 9 30 158 FB-3.............. 2 10 100 42 38 159 FB-4............... 5 9 77 51 36 160 AUBURN 56.......... 9 0 75 33 29 161 FB-5 .............. 17 5 81 72 44 162 FB-6............... 12 3 9 30 14 163 FB-7............... 22 14 50 45 33 164 FB-8 ............... 29 11 85 31 39 165 ROWDEN............. 100 78 98 96 93 18 Steve Stringer, Cotton Branch Station, P. O. Box 789, Marianna, AR 72360 166 CBS-1.............. 7 16 78 54 39 167 CBS-22............ 61 5 56 30 38 168 CBS-3.............. 2 5 39 41 22 169 CBS-4.............. 44 5 77 91 54 170 AUBURN 56.......... 24 2 72 53 38 171 CBS-5.............. 30 11 67 17 31 172 CBS-6.............. 67 7 62 82 54 173 CBS-7.............. 44 4 53 86 47 174 CBS-8.............. 54 8 84 81 57 175 ROWDEN............. 92 73 57 96 80 -10- 1991 FUSARIUM WILT TEST PLANT BREEDING UNIT, TALLASSEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 19 W. C. Johnson, Dept. of Agronomy and Soils, Auburn University, Auburn University, AL 36849 176 COKER 315.......... 100 45 66 65 69 177 STONEVILLE 453..... 32 92 66 97 72 178 DES 119............ 14 41 84 79 55 179 CHEMBRED 219....... 23 33 39 99 48 180 AUBURN 56.......... 15 2 11 90 30 181 STONEVILLE 907..... 47 26 31 100 51 182 HS 46.............. 60 28 41 100 57 183 TERRA C 40......... 17 11 48 91 42 184 PD 3............... 64 43 77 63 62 185 ROWDEN............. 100 69 90 100 90 186 DELTAPINE 50........ 63 17 84 85 62 187 GEORGIA KING....... 67 43 88 58 64 188 SS 35.............. 44 12 88 44 47 189 SUREGROW 1001...... 40 40 90 40 52 190 AUBURN 56........... 10 0 55 56 30 191 STONEVILLE LA 887.. 20 8 73 36 34 192 DELTAPINE 90....... 18 9 89 88 51 193 COKER 320.......... 12 7 88 63 42 194 KC 311............. 46 17 98 73 58 195 ROWDEN............. 75 77 98 99 87 196 DELTAPINE 5415..... 47 17 84 57 51 197 SS 533............. 58 49 99 81 72 198 TERRA 207.......... 21 6 14 18 15 199 HS 23.............. 14 4 57 35 28 200 AUBURN 56.......... 9 9 52 23 23 201 DELTAPINE 5690..... 10 13 76 47 36 202 CHEMBRED 1135...... 14 26 46 38 31 203 COKER 130.......... 9 4 35 41 22 204 DELTAPINE 20....... 8 23 62 21 28 205 ROWDEN............. 68 97 100 82 87 206 DELTAPINE 51....... 22 11 78 76 47 207 CHEMBRED 407....... 40 13 40 94 47 208 TROPICAL 225....... 22 10 71 76 45 209 CB 333............. 34 22 100 82 60 210 AUBURN 56.......... 5 7 61 14 22 211 STONEVILLE 324..... 17 56 100 97 68 212 PAYMASTER HS 26.... 0 1 16 5 6 213 TIFCOT 56.......... 19 40 99 84 60 214 STONEVILLE 825..... 24 97 94 99 78 215 ROWDEN............. 79 94 94 99 92 -11- 1991 FUSARIUM WILT TEST PLANT BREEDING UNIT, TALLASSEE, ALABAMA Test entry Percent wilt by replication designation 1 2 3 4 Mean 20 Freddy Miller, Terra International, P. O. Box 171376, Memphis, TN 38187-1376 216 90-292-91E......... 9 36 10 2 14 217 90-365-91E......... 30 45 40 5 30 21 Dunn Delinting, Rt. 4, Box 431, Seminole, TX 79360 218 D 4000............... 31 23 9 6 17 219 D 1697.............. 62 64 18 4 37 220 AUBURN 56........... 7 8 8 1 6 221 D 18500.............. 35 70 34 58 49 222 D 2461.............. 29 62 59 47 49 223 D 2472.............. 67 32 48 21 42 224 DHS-120............. 85 82 21 7 49 225 ROWDEN............. 100 100 93 70 91 -12-