EI 3iu Progress Report Series No. 44 April 1950 AGRICULTURAL EXPERIMENT STATION of The Alabama Polytechnic Institute, Auburn, Ala. . .* * , M. J. Funchess, Director CONTROL of COTTON INSECTS with DUSTS and SPRAYS F. S. ARANT, Entomologist The boll weevil, Anthonomus grandis Boh., was more abundant and more de- structive in Alabama during 1949 than in any other season during the 23 years that control experiments have been conducted. Other cotton insects were of minor impor- tance, except in localized areas or where populations of cotton aphid, Aphis gossypii Glov., developed from use of calcium ar- senate without an aphicide. The previous winter was exceptionally mild, and a large population of over-win- tering weevils emerged in the spring and early summer. Cloudy weather and fre- quent rains produced ideal conditions for multiplication of boll weevil during the cotton-growing season .. Rainf 11 -mnrde con- trol of insects extremely difficult in some sections. Applications of insecticides were washed off and the soil was frequently too wet to repeat applications with ground ma- chinery before additional rainfall occurred. This progress report gives results of con- trol experiments with organic insecticides in 1949 and summaries of some principal results during the 3-year period, 1947-1949. 1949 EXPERIMENTS Experiments in the control of boll weevil and other cotton pests were performed at five localities in the State, namely: Mon- roeville and Atmore in the southwestern section, Headland in the southeastern area, Prattville in the central area, and Tallassee in the east central part. Dusting at Monroeville The 1949 cotton-growing season was more nearly normal at Monroeville. than in any other part of the State. Although a high population of boll weevil adults emerged from hibernation, weather was favorable during late June and early July for fruiting of cotton. As the over-win- tered adults died, the infestation on cotton declined and approximately one-half bale per acre was set before serious damage occurred. However, dusting was necessary at frequent intervals between July 8 and August 17. A total of nine applications of dust was applied at the rate of approx- imately 12 pounds per acre to cotton on small plots. Treatments were replicated five times in randomized blocks. Infesta- tion counts were made at approximately 5-day intervals and yield records were ta- ken at the end of the experiment. Summarized results of experiments at Monroeville Experiment Field are present- ed in Table 1. Bollworm, Heliothis armng- era (Hbn.), cotton aphid, and red spider mites were minor factors in this experi- ment, except where aphids became numer- ous on plots dusted with calcium arsenate containing no aphicide. Satisfactory con- trol of boll weevil was obtained with all insecticides. However, it was found neces- sary to increase the rate of application of organics above 10 pounds per acre in order to effect control. Gains from dusting rang- ed from 414 pounds per acre on the cal- cium arsenate plots to 1,000 pounds per acre on the areas dusted with 20 per cent toxaphene. Toxaphene, BHC-DDT, and calcium arsenate with nicotine appeared about equally effective. It will be noted that four applications of 20 per cent toxaphene applied June 6 to 18 at the time cotton plants were just beginning to square did not increase yield of cotton. Dusting at Headland The season at Wiregrass Substation, Headland, was somewhat similar to that at Monroeville, except that the weevil in- festation was much higher and rainfall AI I)II II I I II II Al VI1 ll rI L - TABLE 1. SUMMARIZED RESULTS of COTTON-DUSTING EXPERIMENTS, FIVE REPLICATIONS, MONROEVILLE EXPERIMENT FIELD, 1949 Av. Infestation Insects Per Sq. In Yield Seed Cotton Per Acre Treatment 1/ Av. Gain Over B. Weevil Bollworm Aphids R. Spiders Yield Check Pct. Pct. No. No. Lb. Lb. Check --------------------------------------- 73.7 2.0 2.44 2.42 1,742 20% toxaphene, early and late 2/ --------------- 46.5 2.2 0.27 0.34 2,640 898 20% toxaphene, late ----- 47.4 1.1 0.33 0.34 2,742 1,000 BHC-DDT, 3-5 -- ------------------ 49.4 1.3 0.32 1.79 2,548 806 Calcium arsenate alternated BHC-DDT ------------- 50.6 0.9 1.30 2.51 2,508 766 Calcium arsenate alternated calcium arsenate + 2% nicotine - 49.0 0.7 0.95 0.66 2,631 889 Calcium arsenate - 52.4 1.0 6.80 1.18 2,156 414 L. S. D., 5 per cent level 217 1/Dusts all inert, applied approximately 12 pounds per acre: July 8, 15 (rain), 18, 23, 28; August 2, 5, 11, 17 (rain). 2/Dusted June 6, 11 (rain), 13, 18 in addition to the dates given in footnote 1* much more frequent. Also, temperatures procedure. Four check plots, located on were lower, either side of the experimental area, were The experiment was conducted on a field used to estimate the theoretical yield for basis and insecticides were applied by Ne- each plot in the area. gro labor using mule-drawn, 2-row dusters. Almost daily rainfall made application No personnel of the Entomology Depart- of insecticides very difficult. A total of 11 ment was stationed in the vicinity of this applications of dusts was made at the rate experiment, but trips were made at 5- to 7- of 10 to 15 pounds per acre between July 7 day intervals to make infestation counts and August 16. Seven of 11 treatments and give instructions on application of in- were washed off by rainfall within 24 secticides. hours after application. Treatments were replicated four times Summarized results of the experiment in a linear series to simplify the dusting at Headland are presented in Table 2. Boll- TABLE 2. SUMMARIZED RESULTS of COTTON-DUSTING, FOUR REPLICATIONS, NON-RANDOMIZED, WIREGRASS SUBSTATION, 1949 Boll Weevil Bollworm Yield Seed Cotton Treatment 1/ Infestation Infestation Per AcreGain Over Dusted Check Dusted Check Dusted Check Check Pct. Pct. Pct. Pct. Lb. Lb. Lb. Calcium arsenate 74.1 87.4 2.2 2.4 1,125 597 528 BHC-DDT-inert, 3-5 48.1 88.3 0.0 2.9 1,550 448 1,102 20 toxaphene-inert 51.5 87.8 0.1 2.7 1,457 523 934 i/Dusts applied at rate of 10 to 15 pounds per acre with mule-drawn equipment as follows: July 7 (rain), 14 (rain), 18, 22 (rain), 26 (rain), 29 (rain); August 2 (rain), 5, 8 (rain), 11, 16, -2- worm, cotton aphid, and red spider mites were of little importance in this experi- ment. Where calcium arsenate was used without an aphicide, the aphid population was too low to warrant taking infestation counts. The most effective control under the condition of this experiment resulted from use of BHC-DDT mixture, with 20 per cent toxaphene second, and calcium arsen- ate third. This is the second consecutive year that BHC-DDT and toxaphene have been more effective against boll weevil than calcium arsenate at Headland. Dur- ing both years the temperatures were mild. Insect migration was extremely heavy and frequent rainfall made it difficult to keep insecticides on the plants. These condi- tions were quite different from those en- countered at Monroeville where tempera- tures were higher, and very few applica- tions of dusts were washed off by rain. Tractor Spraying and Dusting at Prattville Field-scale experiments were carried on at the Murfee and Dismukes farms near Prattville to determine effectiveness of tox- aphene sprays and dusts for control of cot- ton insects. Insecticides were applied with tractor equipment 1/ to plots approximate- ly 1 to 3 acres in size, depending upon the length of the rows. All treatments were replicated four times. Each replicate was in a separate field. 1/Department of Agricultural Engineering coop- erated in this work. The cotton was planted following a heavy crop of winter legumes in two of the fields. Cotton in these two fields was very rank, reaching a height of 6 to 7 feet on undusted areas. The other two fields had grown no legumes and the plants were con- siderably smaller. Frequent rainfall together with the heavy, sticky nature of the soil made the application of insecticides with ground ma- chinery difficult. A total of 16 applica- tions was made between July 1 and August 30. Seven of these applications were washed off within 24 hours by heavy rain- fall. The insect population was extremely heavy, and it was necessary to shorten the interval between applications of insecti- cides in order to reduce infestation. Sprays were applied at the rate of 6 gallons per acre, while dusts -were applied at approx- imately 12 pounds per acre. Results of the tractor dusting and spray- ing at Murfee and Dismukes farms are pre- sented in Table 3. The toxaphene emul- sion spray and the toxaphene-DDT emul- sion spray appeared to be slightly more effective than the dust in controlling boll weevil. However, the differences in yield from the three treatments were not signifi- cant. The average gains resulting from application of insecticides ranged from 1,043 pounds per acre of seed cotton on the areas dusted with toxaphene to 1,230 pounds on the plots receiving toxaphene- DDT spray. The tractors used for applying sprays TABLE 3. SUMMARIZED RESULTS of TRACTOR SPRAYING and DUSTING of COTTON at MURFEE and DISMUKES FARMS, 1949 Amt. Insects Per Yield Seed Cotton Treatment 1/ Tech- Average Infestation Square Inch Per Acre 2/ nical Boll Boll- Red Av. Gain Over Per Acre Weevil worm Aphids Spiders Yield Check Lb. Pct. Pct. No. No, Lb. Lb. Check......----------------------------. 71.6 77 1.0 0.2 620 Toxaphene dust ------------------ 2.5 3/ 42.7 0.9 0.4 0.1 1,663 1,043 Toxaphene spray -- ---------------- 2 40.7 1.3 0.2 0.1 1,744 1,124 Toxaphene-DDT 2 Tox. spray.------------------------- 1 DDT 35.8 0.2 0.1 0,0 1,850 1,230 L. S. D., 5 per cent level 308 1/Insecticides applied: July 1, 6, 11 (rain), 20 (rain), 25, (rain), 13, 17 (rain), 22, 26, 30 (rain). 2/Based on undamaged rows. 28; August 2 (rain), 3, 6, 10 (rain), 12 3/Approximate rate; varied from 1.6 in first applications to 3 in last. and dusts were equipped with guards de- veloped at the Stoneville Laboratory of the U.S.D.A. Bureau of Plant Industry, Soils, and Agricultural Engineering. T h e s e guards were manufactured, installed, and adjusted by the U.S.D.A. Farm Tillage Machinery Laboratory and the Experiment Station's Department of Agricultural En- gineering. However, some difficulty was encountered in the use of these guards, particularly in crossing terraces with the equipment. Considerable damage occurred to the cotton as a result of the frequent applica- tions of insecticides. Data showing results of this damage are presented in Table 4. It will be noted that the sprayer caused much more severe damage than the duster. The sprayer used was new equipment, which rested low on the back of the trac- tor. Damage was severe in cotton having very rank growth, particularly in fields East I and East II. The large number of applications was another factor in the amount of damage that occurred. How- ever, these data emphasize the need for more suitable machinery for applying in- secticides in Alabama. In many sections, well fertilized land produces a rank growth of cotton. Hilly land and terraces add to the difficulty of effective application of insecticides with ground machinery. Dusting and Spraying at Tallassee Failure to get a stand necessitated aban- doning the field of cotton originally plant- ed for insect-control experiments. A sec- ond planting on a smaller area was mad.e the last week in May. This date is con- sidered by farmers as too late to plant cotton in Alabama and expect a crop. However, the cotton grew rapidly and was not seriously infested with the boll weevil until migration began in early August. In- festation after that time was extremely heavy and the interval of application of insecticides had to be shortened to 3 days ;a s0 -insects. A total of in order to control the insects. total of 12 applications was made between August 9 and September 9. One spray and four dust mixtures were used in the experiments. The spray was a BHC-DDT emulsifiable concentrate con- taining 10 per cent gamma BHC and 16.7 per cent DDT. The concentrate was dilut- ed with water and applied with a hand- sprayer at the rate of approximately 0.36 pounds gamma and 0.6 pounds DDT per acre in 30 gallons of spray. The emulsion tended to settle out in the spray tank unless agitated continuously. Dusts were applied with hand-dusters at the rate of 10 to 15 pounds per acre per application,. Bollworm was a minor factor in this ex- periment and practically no red spiders TABLE 4. EFFECT of TRACTOR DAMAGE on YIELD of SPRAYED and DUSTED COTTON, MURFEE and DISMUKES FARMS, 1949 Yield Of Seed Cotton Per Acre Treatment Gain Dismukes East I East II Church Average Over Check Lb. Lb. Lb. Lb. Lb. Lb. Check.----------------- .......-------------------- 640 508 340 993 620 --- Toxaphene (tractor dust (rows 920 1,556 1,332 1,008 1,204 584 (no tractor (damage -- 1,226 1,836 2,136 1,456 1,663 1,043 Toxaphene (tractor spray (rows 737 1,088 584 844 813 193 (no tractor (damage 1,136 2,228 1,996 1,618 1,744 1,124 Toxaphene- (tractor DDT spray (rows ------ - 831 1,212 1,160 750 988 368 (no tractor (damage 1,490 2,088 2,308 1,515 1,850 1,230 L. S. D., 5 per cent level 308 -o were found. Near the end of the experi- ment, the aphid population on the calcium- arsenate plots was high and the plants lost most of the leaves early enough to reduce the yield of cotton. Summarized results of the dusting and spraying experiment at the Station's Plant Breeding Unit, Tallassee, are presented in Table 5. These results are based on records made from the four inner rows from each plot. All insecticidal treatments reduced boll weevil infestation and increased the yield of seed cotton above that on the un- dusted checks. Twenty per cent toxa- phene dust and BHC-DDT 3-5 dusts were the most effective treatments. Calcium arsenate dust was the least effective treat- ment in terms of seed cotton yield. An aphid population of over 16 insects per square inch of leaf surface developed on plants dusted with calcium arsenate (Table 5), as compared with 3 insects on the un- dusted checks. The leaves dropped from the aphid-infested plants and prevented many of the bolls from maturing properly, thereby reducing yield on the calcium-ar- senate plots. A new material, aldrin. showed promise in the control of the boll weevil in this experiment. The infestation was lower than on the calcium-arsenate plot and no build-up in apids occurred. A gain of 1,066 pounds of seed cotton per acre resulted from the use of 2 per cent aldrin and 5 per cent DDT in an inert car- rier. It is believed from thts experiment that 2 per cent concentration is not quite sufficient for best results from use of al- drin. While the BHC-DDT spray showed promise, it was not as effective as the dust. The high gallonage used in applying the material may have been a factor in the re- sults obtained with the spray. Airplane Dusting and Spraying Experiments were conducted on the con- trol of cotton insects with sprays and dusts applied by airplane at the Murfee and Dis- mukes farms near Prattville and on the At- more State Prison Farm. Prattville. In the experiment near Prattville, 20 per cent toxaphene dust, tox- aphene emulsion spray, and toxaphene oil solution were applied with aerial equip- ment. The insecticide was applied at the rate of 2 pounds of the technical material per acre. A bi-plane was used in applying the spray, with nozzles adjusted to delivei 2 gallons of spray per acre. Dusts were also applied with a bi-plane at the rate ol 10 pounds per acre. Four applications of the insecticides were applied on a field basis between July 1 and 20, and infestation counts of insects were taken at intervals of approximately 5 days. The owner and operator of the plane left the project and went into conm- mercial spraying in Mississippi about July 24. The project was discontinued and nc picking records were made. Average boll weevil infestations during TABLE 5. SUMMARIZED RESULTS of COTTON DUSTING and SPRAYING, FOUR REPLICATIONS, PLANT BREEDING UNIT, TALLASSEE, 1949 Yield Of Seed Treatment 1/ Average Infestation Aphids Per Cotton Per Acre Square Inch Average Gain Over Boll Weevil Bollworm Yield Check Pct. Pct. No. Lb. Lb. Check ------- -- - 88.6 5.87 3.12 643 . ------ 20 toxaphene dust -49.6 1.37 0.15 1,886 1,243 BHC-DDT spray 67.9 1.50 0.18 1,482 839 BHC-DDT, 3-5 dust ------------------- 52.2 1.37 0.09 1,864 1,221 Calcium arsenate ------------------ 65.4 2.37 16.16 1,120 477 2 / aldrin + 5(/, DDT dust -- 62.1 1.50 0.78 1,709 1,066 L. S.S. D., 5 per cent level 282 I/Insecticides applied: August 9 (rain), 13, 16, 19 (rain), 20, 23, 25, 29, 31 (rain); September 2, 7 (rain), 9. 5- the pefiod of spraying are presented in Table 6. All dusts and sprays reduced the infestation of boll weevil, and sprays ap. peared to be equal to the dusts in weevil control. Atmore. Seven applications of insec- ticides were applied with aerial equipmeni on the Atmore Prison Farm between July 29 and August 22. Boll weevil adults were migrating in large numbers throughoul most of this period. Rates of application of insecticides and insect infestations are given in Table 7. Infestation in the area sprayed with toxaphene was slightly lower than where 20 per cent toxaphene dust and 1.5 per cent dieldrin were used. All of the insecticides reduced the boll weevil infesta- tion well below that on the checks. Yield records were not obtained in this experi- ment at Atmore. THREE-YEAR AVERAGE GAINS from DUSTING New organic insecticides have been used experimentally in Alabama over a period of 3 years. In one series of experiments, tests were made to determine effectiveness of insecticides applied during the time the crop was being set and matured. In an- other series, studies were made to deter- mine the relative effectiveness of early and late applications of dusts. Summarized data on both series are herein presented. Dusting While Crop Was Being Set and Matured Four insecticidal treatments have been found to be effective in control of boll wee- vil. Results of all experiments with four leading insecticides, Table 8, show that 20 TABLE 6. AVERAGE BOLL WEEVIL INFESTATIONS in FIELDS DUSTED and SPRAYED with AIRPLANE EQUIPMENT, MURFEE and DISMUKES FARMS, 1949 Amount Of Technical Infestation Treatment 1/ Per Acre 50 A Field Silo Cut East Road Lb. Pct. Pct. Pct. Check --- ------------------------- 0 -- 55.3 57.3 Toxaphene dust ------- ------- ---- 2 47.6 17.2. Toxaphene-oil emulsion 2 21.0 35.8 Toxaphene-oil solution ---- 2 30.6 14.7 39.9 I/Sprays applied at rate of 2 gallons and dusts at 10 pounds per acre per application: July 1, 6, 11 (rain), 20 (rain). TABLE 7. AVERAGE BOLL WEEVIL and BOLLWORM INFESTATIONS in FIELDS DUSTED and SPRAYED with AIRPLANE EQUIPMENT, ATMORE PRISON FARM, 1949 Treatment 1/ Amount Of Technical Infestation Per Acre Boll Weevil Bollworm Lb. Pct. Pct. West check ----------------------------- - 0.0 89.3 1.0 Toxaphene spray ----------------------- 2.0 41.5 0.5 Center check -------------------------- 0.0 76.3 3.0 20% toxaphene dust -----------.. _. 2.0 52.7 0.6 1.5% dieldrin 2/ ----------------- 0.15 47.7 4.3 East check --------------- ---- 0.0 81.7 1.3 I/Insecticides except dieldrin applied: July 29 (rain); August 2, 6 (rain), 11, 15, 19 (rain), 22. 2/Applied: August 6 (rain), 11, 15, 19 (rain), 22. This area had been dusted with toxaphene July 29 and August 2. per cent toxaphene dust and BHC 3-5 mix- ture increased the yield of cotton approx- imately one-half bale per acre in a large number of experiments. Gains from alter- nate applications of calcium arsenate and calcium arsenate containing 2 per cent ni- cotine were considerably less in a smaller number of experiments. Severe damage from bollworm reduced the yield on the calcium-arsenate plots during one season. During another season, very light boll- weevil infestation occurred and the gains were small in all experiments. For these reasons, the average gains from calcium arsenate and calcium arsenate-nicotine are somewhat smaller than might be expected. Where calcium arsenate was used without an aphicide, the average gain from dusting was only 205 pounds of seed cotton per acre. It is realized that the data in Table 8 are taken from a large number of experiments conducted under different conditions. In any exact evaluation of the results, insects present and conditions under which the ex- periments were conducted must be taken into account. However, the averages given in the table are indicative of yields and gains that may be expected from control of cotton insects on good land in Alabama. Tabulated results of experiments, where the four commonly used insecticides oc- curred together in the same field, are pre- sented in Table 9. With one exception gains from dusting are lower than in Table 8, which includes more tests. However, the yields in Table 9 should represent a fair measure of relative efficiency of treat- ments against major cotton pests under Alabama conditions during the 3-year per- iod, 1947-1949. TABLE 8. THREE-YEAR AVERAGE YIELD of SEED COTTON on ALL DUSTED AREAS and CORRESPONDING CHECKS, 1947-1949 Yield Of Seed Cotton Per Acre Treatment Replications Gain From Dusted Undusted Dusting 1/ No. Lb. Lb. Lb. Calcium arsenate ----------------- 36 1,749 1,536 213 Calcium arsenate alternate with calcium arsenate-2% nicotine 25 2,135 1,821 314 BHC-DDT, 3-5 dust 39 2,085 1,425 660 20 ( toxaphene dust-------------- 73 2,103 1,349 754 1/These gains may not be strictly comparable, since all treatments did not always occur together in the same experiment. TABLE 9. THREE-YEAR AVERAGE YIELD of SEED COTTON in ALL EXPERIMENTS WHERE FOUR INSECTICIDES OCCURRED TOGETHER in the SAME TEST, 1947-1949 Treatment Replications Yield Of Seed Cotton Per Acre Yield Gain Over Check No. Lb. Lb. Check --- 21 1,946.211 Calcium arsenate -- 21 2,048 102 Calcium arsenate alternate with calcium arsenate-2 (' nicotine ------ 21 2,292 346 BHC-DDT, 3-5 dust 21 2,385 439 20( toxaphene dust------------------ 21 2,459 513 -7- Timing Applications of Dusts For many years entomologists and farm- ers alike have been interested in the possi- bility of applying insecticides early to kill over-wintered boll weevils. In theory, the elimination of these over-wintered adults should reduce damage later in the year. In practice, profitable control of boll weevil has not resulted from use of calcium arsen- ate in the form of pre-square mopping and pre-square dusting. Introduction of new organics again fo- cused attention upon early application of insecticides. Early application on county- wide basis has been recommended in some sections of Texas. Experiments have been conducted in Alabama during the past 3 years in order to determine the effective- ness of early applications with new insec- ticides. In general, the procedure has been to apply three to four applications of insec- ticides at 5-day intervals, beginning just before the first squares are large enough for boll weevil egg punctures. This pro- cedure should permit a maximum number TABLE 10. EFFECT of TIME of APPLICATION of 20 PER CENT TOXAPHENE DUST on INSECT CONTROL and COTTON YIELD, FOUR to FIVE REPLICATIONS, 1947-1949. Average Insects Per Yield Of Seed Year Treatment Infestation Square Inch Cotton Per A. Boll Boll- Red Weevil worm Aphids Spider Yield Gain Pct. Pct. No. No. Lb. Lb. Undusted check .............---------------- 48.5 12.8 7.1 .... 3/ 762 3 dustings, June 17- 1947 1/ July 2 2/ ...........------------------- 45.8 18.6 7.3 ---- 629 -133 5 dustings, July 11- August 21 2/ --------------- 20.0 4.5 2.2 1,165 403 Undusted check ........---------------- 45.8 ..... 3/ 2.7 ---- 3/ 1,616 3 dustings, June 2-12 ------ 38.8 1.7 - 1,609 -7 3 dustings, June 2-12, 1948 plus 7 dustings, July 12- August 14 ......... ..------------- 26.7 -- 0.7 2,018 402 7 dustings, July 12- August 14 --- .. ...--------------- 24.6 0.7 ---- 2,075 459 Undusted check .----------------. 54.7 5.5 0.3 1,760 3 dustings, June 1-15, 1948 plus 7 dustings, July 7- August 12 --------..----------. . 48.9 3.0 2.9 1,892 132 7 dustings, July 7- August 12 ----- .........------------- 46.8 3.3 1.1 1,898 138 Undusted check ---------------- 73.7 2.0 2.4 2.4 1,742 4 dustings, June 6-18, 1949 4/ plus 9 dustings, July 8- August 17 -------------------- 46.5 2.2 0.3 0.3 2,640 898 9 dustings, July 8- August 17 ..----------- - - 47.4 1.1 0.3 0.3 2,742 1,000 1/Population of over-wintered weevils June 14 averaged 502 per acre over the experimental areas. 2/Plus one application washed off. 3/Practically no damage. 4/Population of over-wintered weevils averaged 130 per acre over the experimental area June 6 before dusts were applied. of over-wintered weevils to emerge and then kill the insects with insecticides before they have an opportunity to infest cotton squares. Results of field-scale and small-plot ex- periments on timing of applications are presented in Table 10. No gains have re- sulted from the early applications in Ala- bama. During one season, the plots re- ceiving three early dustings produced less cotton than undusted checks in each of four fields near Prattville. The reduction in yield resulted from bollworm coming into the area after the dusting operation had ceased. Insect predators had been destroyed by the insecticides and boll- worms caused more damage on dusted than on undusted areas. In all other tests, the yields on plots treated early have been slightly but not significantly lower than those on the corresponding plots receiving no early application. It is concluded from these experiments that insecticides applied to young cotton having a few or no squares were ineffec- tive in increasing the yield. There is evi- dence that setting of bolls occurred a little earlier where the early applications were made. SUMMARY Gains in cotton yields resulting from dusting and spraying for control of boll weevil, Anthonomus grandis Boh., and other cotton pests ranged from 766 to 1,243 pounds of seed cotton per acre on well fer- tilized land in 1949, a season of extremely high insect populations and adverse weath- er conditions. Materials giving the most effective control and highest yields were: (1) 3 per cent gamma benzene hexa- chloride-5 per cent DDT dust, (2) 20 per cent toxaphene dust, and (3) sprays pre- pared from emulsifiable concentrates of toxaphene or toxaphene and DDT. Dust containing 2 per cent aldrin and 5 per cent DDT was effective, but plots re- ceiving this mixture yielded slightly less cotton than plots treated with toxaphene or BHC-DDT dust. A spray prepared from an emulsifiable concentrate of BHC and DDT was somewhat less effective than tox- aphene sprays. Dieltrin applied as a 1.5 per cent dust showed promise against boll weevil. Over a 3-year period, 73 replications of 20 per cent toxaphene dust resulted in an average gain of 754 pounds of seed cotton per acre over undusted cotton; 39 replica- tions of BHC-DDT mixture resulted in a gain of 660 pounds of seed cotton per acre. Thirty-six replications of calcium arsenate without an aphicide resulted in a gain of 213 pounds of seed cotton per acre. Three-year average results on timing of applications of insecticides on cotton re- sulted in yields as follows: Cotton receiv- ing three applications before and just after squaring begins without later treatment averaged 70 pounds of seed cotton per acre less than cotton receiving no insecti- cide. Where three early applications were followed by an average of seven dustings during the time the crop was being set and matured, the average gain was 483 pounds of seed cotton per acre over undusted areas. Cotton receiving no early treat- ment, but dusted an average of seven times while the crop was being set and matured, produced 508 more pounds of seed cotton per acre than undusted areas-in the same experiments. -9-